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Selection of transthyretin amyloid inhibitorsIakovleva, Irina January 2016 (has links)
Amyloidosis is a group of clinical disorders caused by the aggregation of specific proteins into abnormal extracellular deposits. Today, 31 different proteins have been linked to amyloid diseases including transthyretin-related amyloidosis (ATTR). ATTR occurs through the aggregation of either wild-type plasma protein transthyretin (TTR) or a mutated form. TTR is a homotetramer that under normal circumstances functions as a carrier of thyroxine and retinol binding protein. The aggregation cascade requires dissociation of the tetramer into monomers, and preventing this dissociation represents a potential mode of intervention. Interestingly, small molecules, referred as kinetic stabilizers, can bind to TTR’s thyroxine-binding site (TBS) and such molecules are currently being used as a therapeutic approach to impair tetramer dissociation. The efficacy of TTR stabilization is directly correlated to the binding affinity of the ligand to TBS. However, the binding of the ligand to TTR in vivo can be affected by other plasma components resulting in poor efficacy. Thus, the selectivity of ligands is an important parameter. We have designed an assay where the ability to stabilize TTR can be directly evaluated in plasma and we have investigated the stabilizing effect of nine potential TTR binders (Paper I). The results, surprisingly, revealed that the binding affinity of molecules has a poor correlation to its selectivity. However, the nature of protein-ligand complex formation can also be described by enthalpic (∆H) and entropic (∆S) energy contributions. ∆H represents the change in chemical bonds and frequently requires a higher order of orientation compared to the ∆S component, which mainly represents the hydrophobic effect via the exclusion of water. We hypothesized that ligands possessing high ΔH in binding to their co-partner would also be more specific in a complex environment such as plasma. By applying a thermodynamic analysis using isothermal titration calorimetry, we found that the selectivity in plasma correlates well with the ∆H contribution and might, therefore, be a better predictor for selectivity. Luteolin was found to be a highly selective stabilizer of TTR and was investigated further (Paper II). The ligand displayed a significant rescuing effect in both cell culture and animal models. However, luteolin undergoes rapid enzymatic degradation in the liver and this impairs its use as a potential therapeutic drug. To attempt to circumvent this issue, we modified the most exposed hydroxyl group thus rendering the molecule inert towards glucuronidation (Paper III). The substitutions resulted in higher stability in the face of hepatic degradation molecules, but they also affected the selectivity in a negative manner. The screening for new TTR stabilizers resulted in the discovery of tetrabromobisphenol A, which displayed a very high selectivity (Paper IV). This study also included a comparison with the drug Vyndaqel™ which currently is in clinically use, and showed how the dosage could be altered to acquire a better level of saturation and possibly also a better clinical effect. Taken together we present new molecules with the ability to stabilize TTR, and these can serve as scaffolds for the design of new drugs. We present a method to measure the efficacy of a TTR-stabilizing drugs in a complex matrix and as well as a way to adjust the dosage of existing drugs. We also show that the selectivity of a drug is affected by the relative proportion of ∆H and ∆S, and this is of interest for drug design in general.
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The role of non-coding genetic variants on transthyretin gene transcription in transthyretin amyloidosisBoldbaatar, Batbold 03 March 2021 (has links)
The transthyretin-associated amyloidoses are a group of protein-folding disorders caused by deposition of the liver-secreted plasma protein transthyretin (TTR) in various tissues of the body. The sporadic form of the disease is caused by deposition of wild-type TTR whereas the inherited form is caused by deposition of mutated TTR; there are over 100 known amyloidogenic mutations of the TTR gene. The transcriptional regulation of the mouse transthyretin gene has been well studied. Organ-specific modulation of TTR mRNA is achieved by coordinated binding of hepatocyte-specific and ubiquitously expressed transcription factors to regulatory regions in the proximal promoter and upstream enhancer region. The hypothesis of this dissertation is that non-coding genetic sequence variations in the promoter of the transthyretin gene situated upstream of the regulatory regions, alter its transcriptional regulation, contributing to the onset and expression of transthyretin-associated amyloidosis. Previously, we identified a significant association of a non-coding polymorphism of the TTR promoter, rs3764479, with age of onset and survival in patients with ATTRwt amyloid disease. In this dissertation, electrophoretic mobility shift assays (EMSA) were used to investigate transcription factor binding of HepG2 nuclear proteins to short DNA probes with and without rs3764479. These mobility shift studies revealed that HepG2 nuclear extract proteins showed higher affinity to the wild-type TTR sequence than to one containing the rs3764479 SNP. Competition EMSAs suggested SNP-related changes in the binding of transcription factors hepatocyte nuclear factor-1 (HNF1) and hepatocyte nuclear factor-3b may alter TTR gene transcription. To investigate transthyretin gene regulation in V122I ATTR amyloid, the most prevalent TTR gene variant in the United States, the proximal promoter region from patients with V122I ATTR amyloidosis was sequenced and analyzed. In total, 8 SNPs were identified; one (rs955705399) was significantly associated with disease between the two V122I genotype-positive cohorts studied with and without cardiomyopathy. It is postulated that the presence of SNPs could influence gene expression and ultimately disease pathogenesis. In summary, these studies suggest that presence of disease-associated non-coding genetic variations modify transthyretin gene expression by disrupting transcription factor binding and may, in part, explain the clinical heterogeneity seen in patients with transthyretin-associated amyloidoses.
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Anticoagulation treatment in patients with a mechanical heart valveGrzymala-Lubanski, Bartosz January 2016 (has links)
Background Every year about 2,500 patients in Sweden undergo surgery for heart valve disease, primarily in the aortic valve. In contrast to the mitral valve, which can be repaired in 70% of the cases, the aortic valve is normally replaced by a mechanical or biological prosthesis. A mechanical heart valve (MHV) necessitates lifelong anticoagulation treatment with a vitamin K antagonist, most commonly warfarin, due to the high thrombogenicity of the prosthesis. The quality of the warfarin treatment is crucial in these patients. Compared to other countries, treatment quality in Sweden is very high; nonetheless, there is always room for improvement. One of the ways to achieve this improvement is to implement computerized dosing assistance. Treatment recommendations for anticoagulation intensity are based on few and old studies, making these recommendations uncertain. There is therefore a need for studies designed to establish the appropriate level of anticoagulation therapy. Aim The aim of these studies was to investigate the efficacy and safety of anticoagulation treatment among patients with mechanical heart valve prostheses in Sweden; to assess whether computerized dosing can increase the treatment quality; to investigate the influence of the treatment quality, measured by Time in Therapeutic Range (TTR) and INR variability, on the risk of complications and, finally, to establish the optimal intensity of anticoagulation treatment in this group of patients. Methods Data were obtained from AuriculA – a national quality registry established in 2006, which currently includes approximately 50% of all patients treated with oral anticoagulation in Sweden. Study II used only data from AuriculA. 769,933 warfarin-dosing suggestions proposed by the dosing algorithm in AuriculA were analysed. Accepted dose suggestions (590,939) were compared with 178,994 manually-changed doses in regard to the resultant INR value, measured as mean error (deviation from target INR) and hit rate (number of INR samples within the target range 2-3). In study III, AuriculA was used to identify patients in Sundsvall and Malmö in the period 2008 – 2011 who were receiving warfarin for a mechanical heart valve prosthesis, as well as to retrieve their INR data. Data on background characteristics and bleedings or thromboembolic complications were manually retrieved from medical records by two investigators. A total of 534 patients with mechanical heart valve prostheses were divided into quartiles based on TTR and were compared regarding the risk of complications. For Studies I and IV, data from AuriculA were merged with the Swedish National Patient Register, SWEDEHEART/ Heart surgery, and the Swedish Cause of Death Register, comprising in total 77,423 patients on warfarin with 217,804 treatment years. Every treatment period registered in AuriculA was given an individual identification number. During the study period a patient could have any number of treatment periods. The number of complications in total and in different patient groups within the study population was investigated. Complications were defined by ICD-10 codes. Major bleeding was defined as an event necessitating hospital treatment and given a discharge diagnosis with one of the ICD-10 codes reflecting bleeding, as listed in the Appendix. Bleeding events were divided into intracranial, gastrointestinal and other bleedings. Thromboembolic complications consist of venous events (deep vein thrombosis, pulmonary embolism, venous stroke) or arterial events (stroke, TIA, acute myocardial infarction, peripheral arterial embolism). Data were analysed using both simple, descriptive statistical methods and various tests such as Mann-Whitney (or two sample Wilcoxon), T-test, Chi 2 test, ANOVA, multivariate analysis with logistic regression and survival analysis with Cox Regression with proportional hazard assumption. Results Treatment quality Mean TTR among all patients in Study I was 76.5% whereas patients with mechanical heart valve prostheses had a TTR of 74.5%. The annual incidence of major bleeding or thromboembolic events among all patients was 2.24% and 2.65%, respectively. The incidence of intracranial bleeding was 0.37% per year in the general population and 0.51% among patients with mechanical heart valve prostheses, who also had a higher bleeding rate in total (3.37% per year). Both the mean and median errors were smaller (0.44 vs. 0.48 and 0.3 vs. 0.4, respectively) and the hit rate was higher (0.72 vs. 0.67) when the dose suggested by the algorithm was accepted, compared to when it was manually changed. TTR In Study III there was no significant difference in the risk of thromboembolism regardless of TTR level. Risk of bleeding in quartiles I and II was more than two times higher than in the quartile with TTR >82.9. In Study IV, lower TTR (≤70%) was associated with a significantly higher rate of complications when compared with TTR >70%. Bleeding risk was higher in the group with lower TTR (HR=2.43, CI 2.02-2.89, p<0.001). After dividing patients into TTR quartiles, the rate of complications in total was significantly higher in quartiles I to III compared with quartile IV, which had the highest TTR. Risk of thromboembolism, major bleeding and death was higher in the first and second quartile compared to the quartile with the highest TTR. INR variability Higher INR variability above mean (≥0.40) was related to a higher rate of complications compared with lower INR variability (<0.40) as shown in Study IV. Bleeding risk was higher in the group with INR variability ≥0.40 (HR = 2.15, CI 1.75-2.61, p<0.001). Comparison of quartile IV, which had the lowest INR variability, with the other three revealed that quartiles I and II, which had the highest INR variability, had significantly worse outcomes for all complications except for thromboembolic events, plus also death in quartile II. TTR and INR variability combined High variability and low TTR combined was associated with a higher risk of bleedings (HR 2.50, CI 1.99-3.15), death (3.34, CI 2.62-4-27) and thrombosis (1.55, CI 1.21-1.99) compared to the best group. Level of anticoagulation Higher warfarin treatment intensity (mean INR 2.8-3.2 vs. 2.2-2.7) was associated with a higher rate of bleedings (HR 1.29, CI 1.06-1.58), death (1.73, CI 1.38-2.16) and complications in total (1.24, CI 1.06-1.41) after adjustment for MHV position, age and comorbidity. Conclusion Warfarin treatment quality is crucial for patients with mechanical heart valve prostheses. Computerized dosing assistance could help maintain high warfarin treatment quality. Well-managed treatment with TTR ≥70% and INR variability below mean <0.40 is associated with a lower risk of serious complications compared with a lower TTR and higher INR variability. No benefit of higher warfarin treatment intensity was found for any valve type or position.
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Optimization of the structure of TTR Ligands for Half-life Extension (TLHE)Jiang, Guanming 01 January 2022 (has links)
Many potential therapeutic agents face challenges for their clinical development due to short circulation half-life. As a result, prolonging the half-life of therapeutic drugs in circulation while preserving their hydrophilicity and small size will be a key step toward more effective and safe pharmacological molecules. Our lab developed a new approach for enhancing the safety and efficacy of therapeutic agents. By endowing therapeutic agents with a hydrophilic small molecule (a derivative of the clinical candidate, AG10) which reversibly binds to the serum protein transthyretin (TTR), the half-life of the therapeutic agent should be extended by binding to the TTR in serum. We termed this technology TTR Ligand for half-life extension (TLHEs). The approach involved using TLHE, which binds with TTR by high specificity and affinity. Our group has already shown that this technology extends the half-life of peptides, small molecules, and proteins without seriously affecting their binding activity towards their receptor and efficacy. As we are expanding the applicability of TLHE to extend the half-life of hydrophobic moieties, increasing the polarity of the TLHE linker could be beneficial to maintain overall hydrophilicity. Our main objective here is to see the effect of TTR binding affinity and selectivity of TLHE in serum when we attach a hydrophilic glutamic acid in the TLHE linker.
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Evaluation of novel fluorescent probes for in vivo Transthyretin amyloid using fibrils generated in vitro under varying conditionsDuong, Sun January 2019 (has links)
Transthyretin (TTR) amyloidosis is a disease that appears in three variants. One variant affects the elderly population with heart failure, the other two variants are hereditary and caused by an amino acid substitution in the gene, resulting in polyneuropathy and/or heart issues depending on the amino acid substitution. However, in all three variants, other organs may also be affected with amyloid deposition in the disease course. Amyloid fibrils of TTR (ATTR) contains a mixture of full-length protein and fragments (50-127). Luminescent conjugated oligothiophenes (LCO’s) are novel amyloid binding probes used to stain amyloid fibrils and these amyloid probes have the feature of characterizing the amyloid structure in terms of fluorescence spectra. Apart from LCO’s, a few other amyloid binding probes are used to stain recombinant amyloid transthyretin and native transthyretin for binding studies. The majority of generated TTR aggregates in vitro did not have the characteristic fluorescence spectra when bound to LCO’s and was observed as a clumped gel-like aggregate. The generation of recombinant TTR fibrils in vitro using the mutant TTR-T49M to obtain an aggregation prone fragment (50-127) after being treated with cyanogen bromide had a low yield of in vivo amyloid-like fibrils, but with characteristic LCO spectra. Carpal tunnel ATTR often precedes ATTR deposition in heart tissue. Amyloid transthyretin in carpal tunnel tissues was stained with LCO’s and used as a reference in the comparison against the in vitro generated recombinant amyloid transthyretin fibrils. This project also includes quantification of amyloid transthyretin in a few selected parts of the carpal tunnel tissue using ImageJ. In the long run this method could help in diagnosing TTR amyloidosis.
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Engelsk språkinlärning och läromedel : En kvalitativ läromedelsanalys av Happy – Textbook year 3Björnfot, Therese, Kujala, Pia January 2017 (has links)
Syftet med föreliggande studie är att med utgångspunkt i relevant forskning analysera om läromedel, som används i engelskundervisningen i årskurserna F-3, med tillhörande sångtexter, rim och ramsor stöder elevers språkinlärning i engelska. Metoder som använts i studien var dels en delstudie om vilket läromedel i engelskundervisningen kommunala skolor i Sverige använder i årskurserna F-3. Därutöver gjordes en bild- och textanalys av materialets innehåll samt jämfördes materialets verb med Corpus of Contemporary American English (COCA) (Davies, 2008-) och metoden type/token-ratio (TTR) för att kunna jämföra den inbördes variationen av verb och substantiv i materialet. Resultatet visar att läromedlet stöder språkinlärningen hos elever i de yngre åldrarna. Majoriteten av verben i materialet fanns med bland de 300 mest frekventa verben i COCA (Davies, 2008-) och skillnaden mellan de undersökta ordklasserna var inte lika stora som befarat. Materialet är i linje med och stöds av kursplanen för engelskämnet i Lgr 11 (Skolverket, 2011b), dock med få undantag. Språket i materialet är mestadels skrivet i presens. Slutsatsen som kan dras är att läromedel med tillhörande sånger, rim och ramsor kan stödja elevers språkinlärning i engelska.
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Métabolisme des rétinoïdes et facteurs d’influence dans l’obésité, la résistance à l’insuline et le diabète de type 2 : études chez l’animal et chez l’hommeSima, Aurelia 05 1900 (has links)
No description available.
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Desenvolvimento de métodos para a quantificação direta de Salmonella sp. por PCR-tempo real e por transcriptase reversa-PCR-tempo real / Development of methods for the direct quantification of Salmonella sp. using real time-PCR and reverse transcriptase-PCR-real timeFroder, Hans 25 November 2008 (has links)
Para obter resultados rápidos e confiáveis que permitam o monitoramento da segurança microbiológica de alimentos, seja pela indústria ou pelos órgãos de fiscalização, diversos métodos alternativos têm sido desenvolvidos para a detecção e quantificação de Salmonella. Os propósitos do estudo foram avaliar a viabilidade de emprego do QIAamp® DNA Stool Mini Kit para extração e purificação de DNA de Salmonella; validar ensaios baseados em PCR-tempo real (PCR-RT) para quantificar o DNA de Salmonella empregando ttr ou tuf e desenvolver um ensaio para quantificar Salmonella baseado na transcriptase reversa-PCR-tempo real (RT-PCR-RT). Para avaliação do QIAamp® DNA Stool Mini Kit empregaram-se fezes coletadas diretamente do reto de animais infectados ou não, sendo estas últimas artificialmente contaminadas e submetidas à extração segundo protocolo do fabricante. As amostras de DNA isoladas foram quantificadas empregando um ensaio Salmonella-específico PCR-RT utilizando como alvo o lócus ttr. O mesmo ensaio foi utilizado para células de Salmonella provenientes de meio de cultura. O ensaio PCR-RT baseado no alvo tuf foi validado empregando-se primeiramente cepas de diferentes sorotipos de Salmonella e de outras Enterobacteriaceae. A seguir sua eficiência foi avaliada para alimentos-modelo (ave e suíno) artificialmente contaminadas com elevada (≈ 6 log UFC/mL) e baixa (≈ 2 log UFC/mL) população de Salmonella Typhimurium DT 104. A validação do método quantitativo de Salmonella por RT-PCR-RT foi realizada primeiramente com células em meio de cultura e posteriormente nos mesmos alimentos-modelo utilizados para PCR-RT. Em ambos os métodos, alíquotas dos alimentos-modelo foram mantidas a 20 ºC e a 8 ºC, sendo examinadas em diferentes tempos pós-inoculação. Como controle empregou-se a enumeração de microrganismos mesófilos totais e de Salmonella por técnicas convencionais. A taxa de recuperação de Salmonella em fezes suínas artificialmente inoculadas, após tratamento com QIAamp® DNA Stool Kit, variou entre 25% a 50%, dependendo da quantidade inicial de células. Empregando o DNA extraído e submetendo-o à PCR-RT para o ttr obteve-se limite de detecção de 2,8 log UFC eq/g de fezes; método que foi menos sensível que o convencional. A quantificação de Salmonella por PCR-RT empregando tuf apresentou limite de detecção menor que 1 log UFC eq. Os resultados obtidos com este método, empregando-se células em meio de cultura ou alimentos-modelo, foram, de maneira geral, ligeiramente inferiores aos do método convencional. A eficiência de amplificação para PCR-RT e tuf foi de 94%. O método RT-PCR-RT apresentou limite de detecção semelhante ao obtido com o ttr (2 log UFC eq) e sua eficiência de amplificação foi de 100%. Observou-se que tuf é expresso na fase logarítmica de multiplicação bacteriana, o que o torna um bom indicador da viabilidade de Salmonella. / In order to get fast and trustworthy results that allow monitoring the microbiological food safety either by industries or governmental agencies, diverse alternative methods have been developed for Salmonella detection and quantification. The purposes of this study were to evaluate the viability of the use of QIAamp® DNA Stool Mini Kit for Salmonella DNA extraction and purification; to validate assays based on real time-PCR (PCR-RT) to quantify Salmonella DNA by using ttr or tuf, and to develop an assay to quantify Salmonella based on reverse transcriptase- PCR-real time (RT-PCR-RT). For QIAamp® DNA Stool Mini Kit evaluation feces taken directly from the rectum of infected or health animals were used, with the former being artificially contaminated. Samples were submitted to DNA extraction, according to manufacturers protocol. The isolated DNA were quantified using a Salmonella-specific PCR-RT targeting the ttr locus. The same assay was used for Salmonella cells originated from culture medium. The PCR-RT assay with tuf as target was first validated employing different Salmonella serovars and other Enterobacteriaceae strains. After, its efficiency was evaluated on food-models (chicken and swine) spiked with high (≈ 6 log CFU/mL) and low (≈ 2 log CFU/mL) Salmonella Typhimurium DT 104 populations. The validation of the quantitative RT-PCR-RT method was first conducted with cells grown in culture medium, and then in the same food-model used for PCR-RT. For both methods aliquots of foodmodels were maintained at 20 ºC and 8 ºC being evaluated at different incubation times. Enumeration of total mesophilic microorganisms and Salmonella based on conventional methods were used as controls. The DNA recovery rate in swine feces artificially inoculated, after QIAamp® DNA Stool Mini Kit treatment, was between 25% to 50% depending the initial amount of cells. Using the extracted DNA and submitting it to PCR-RT for ttr a detection level of 2,8 CFU eq/g of feces was obtained. This method showed lower sensitivity than the conventional. Salmonella quantification by PCR-RT employing tuf showed a detection level lower than 1 log CFU eq. The results obtained with this method and cells suspended in culture medium or in food-model systems were, in general slightly lower that those obtained with the conventional method. The efficiency of amplification for PCR-RT tuf was 94%. Detection limit of RT-PCR-RT was similar to that of ttr (2 log CFU eq) and efficiency of amplification was 100%. tuf was expressed in logarithmic phase of bacteria growth curve showing that it is a good viability indicator for Salmonella.
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Desenvolvimento de métodos para a quantificação direta de Salmonella sp. por PCR-tempo real e por transcriptase reversa-PCR-tempo real / Development of methods for the direct quantification of Salmonella sp. using real time-PCR and reverse transcriptase-PCR-real timeHans Froder 25 November 2008 (has links)
Para obter resultados rápidos e confiáveis que permitam o monitoramento da segurança microbiológica de alimentos, seja pela indústria ou pelos órgãos de fiscalização, diversos métodos alternativos têm sido desenvolvidos para a detecção e quantificação de Salmonella. Os propósitos do estudo foram avaliar a viabilidade de emprego do QIAamp® DNA Stool Mini Kit para extração e purificação de DNA de Salmonella; validar ensaios baseados em PCR-tempo real (PCR-RT) para quantificar o DNA de Salmonella empregando ttr ou tuf e desenvolver um ensaio para quantificar Salmonella baseado na transcriptase reversa-PCR-tempo real (RT-PCR-RT). Para avaliação do QIAamp® DNA Stool Mini Kit empregaram-se fezes coletadas diretamente do reto de animais infectados ou não, sendo estas últimas artificialmente contaminadas e submetidas à extração segundo protocolo do fabricante. As amostras de DNA isoladas foram quantificadas empregando um ensaio Salmonella-específico PCR-RT utilizando como alvo o lócus ttr. O mesmo ensaio foi utilizado para células de Salmonella provenientes de meio de cultura. O ensaio PCR-RT baseado no alvo tuf foi validado empregando-se primeiramente cepas de diferentes sorotipos de Salmonella e de outras Enterobacteriaceae. A seguir sua eficiência foi avaliada para alimentos-modelo (ave e suíno) artificialmente contaminadas com elevada (≈ 6 log UFC/mL) e baixa (≈ 2 log UFC/mL) população de Salmonella Typhimurium DT 104. A validação do método quantitativo de Salmonella por RT-PCR-RT foi realizada primeiramente com células em meio de cultura e posteriormente nos mesmos alimentos-modelo utilizados para PCR-RT. Em ambos os métodos, alíquotas dos alimentos-modelo foram mantidas a 20 ºC e a 8 ºC, sendo examinadas em diferentes tempos pós-inoculação. Como controle empregou-se a enumeração de microrganismos mesófilos totais e de Salmonella por técnicas convencionais. A taxa de recuperação de Salmonella em fezes suínas artificialmente inoculadas, após tratamento com QIAamp® DNA Stool Kit, variou entre 25% a 50%, dependendo da quantidade inicial de células. Empregando o DNA extraído e submetendo-o à PCR-RT para o ttr obteve-se limite de detecção de 2,8 log UFC eq/g de fezes; método que foi menos sensível que o convencional. A quantificação de Salmonella por PCR-RT empregando tuf apresentou limite de detecção menor que 1 log UFC eq. Os resultados obtidos com este método, empregando-se células em meio de cultura ou alimentos-modelo, foram, de maneira geral, ligeiramente inferiores aos do método convencional. A eficiência de amplificação para PCR-RT e tuf foi de 94%. O método RT-PCR-RT apresentou limite de detecção semelhante ao obtido com o ttr (2 log UFC eq) e sua eficiência de amplificação foi de 100%. Observou-se que tuf é expresso na fase logarítmica de multiplicação bacteriana, o que o torna um bom indicador da viabilidade de Salmonella. / In order to get fast and trustworthy results that allow monitoring the microbiological food safety either by industries or governmental agencies, diverse alternative methods have been developed for Salmonella detection and quantification. The purposes of this study were to evaluate the viability of the use of QIAamp® DNA Stool Mini Kit for Salmonella DNA extraction and purification; to validate assays based on real time-PCR (PCR-RT) to quantify Salmonella DNA by using ttr or tuf, and to develop an assay to quantify Salmonella based on reverse transcriptase- PCR-real time (RT-PCR-RT). For QIAamp® DNA Stool Mini Kit evaluation feces taken directly from the rectum of infected or health animals were used, with the former being artificially contaminated. Samples were submitted to DNA extraction, according to manufacturers protocol. The isolated DNA were quantified using a Salmonella-specific PCR-RT targeting the ttr locus. The same assay was used for Salmonella cells originated from culture medium. The PCR-RT assay with tuf as target was first validated employing different Salmonella serovars and other Enterobacteriaceae strains. After, its efficiency was evaluated on food-models (chicken and swine) spiked with high (≈ 6 log CFU/mL) and low (≈ 2 log CFU/mL) Salmonella Typhimurium DT 104 populations. The validation of the quantitative RT-PCR-RT method was first conducted with cells grown in culture medium, and then in the same food-model used for PCR-RT. For both methods aliquots of foodmodels were maintained at 20 ºC and 8 ºC being evaluated at different incubation times. Enumeration of total mesophilic microorganisms and Salmonella based on conventional methods were used as controls. The DNA recovery rate in swine feces artificially inoculated, after QIAamp® DNA Stool Mini Kit treatment, was between 25% to 50% depending the initial amount of cells. Using the extracted DNA and submitting it to PCR-RT for ttr a detection level of 2,8 CFU eq/g of feces was obtained. This method showed lower sensitivity than the conventional. Salmonella quantification by PCR-RT employing tuf showed a detection level lower than 1 log CFU eq. The results obtained with this method and cells suspended in culture medium or in food-model systems were, in general slightly lower that those obtained with the conventional method. The efficiency of amplification for PCR-RT tuf was 94%. Detection limit of RT-PCR-RT was similar to that of ttr (2 log CFU eq) and efficiency of amplification was 100%. tuf was expressed in logarithmic phase of bacteria growth curve showing that it is a good viability indicator for Salmonella.
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