This thesis is a feminist reading of the work of Henry Handel Richardson (1870-1946), which considers her four major novels: Maurice Guest (1908), The Getting of Wisdom (1910), The Fortunes of Richard Mahony (1930), and The Young Cosima (1939). It proposes that Richardson foregrounds the work of gender ideology in her novels, and that her work is also conscious about its own fictional procedures. This thesis argues that Richardson consciously examines the ideological aspect of narrative modes, such as naturalism, the Bildungsroman, and popular romance. Moreover, it illustrates her attempts to invent narrative strategies which subvert the conventional assumptions about gender inherent in those forms. ???Gender Ideology and Narrative Form??? draws on recent theoretical approaches to narrative, ideology, subjectivity, and dialogism, to argue that Richardson makes the ideological shaping of her stories most visible through manipulations of genre, plot, narrative voice, and point of view. Aspects of ideology examined include the Victorian and late-Victorian equation of masculinity with public rationality, mind, public achievement, and genius: and, on the other hand, the association of femininity with the body, passion, and private or domestic spaces. The thesis also considers some of the values and assumptions about gender implicit in nineteenth-century scientific thinking. Henry Handel Richardson has been viewed as a conservative writer, in both aesthetic and political terms. By contrast, I suggest that she resists the moral and representational codes of the realist or naturalist form, and that her uncompromising oppositional strategy achieves a number of radical results. It exposes and criticises the masculinist bias of certain representational methods; it offers new ways of representing female experience; and it insists that the private sphere must be treated also as a political space in which crucial power relationships are at work. My approach to Henry Handel Richardson???s fiction opens new ways to see her work as the product of a distinctive feminist consciousness.

This thesis demonstrates the close links between Australian literature and German thought and culture in Catherine Martin's An Australian Girl (1890) and Henry Handel Richardson's Maurice Guest (1908), and thereby provides a fuller understanding of the sophisticated literary and intellectual purposes of these two works. In examining the German elements in each novel, and the contexts from which much of that material is drawn, this study seeks to supplement the scholarly explanations provided in the two Academy Editions of these works. While Maurice Guest has received serious scholarly attention, An Australian Girl has been accorded relatively little. Despite generally favourable reviews on publication, both appear to have been undervalued over time. The study begins with a brief historical survey of German migration to Australia and the contribution German migrants made to the intellectual life and culture of the evolving nation. The examination of Catherine Martin's work includes: biographical details, particularly concerning her contact with German culture; an analysis of the form of the novel and a comparison of An Australian Girl with Goethe's Bildungsroman Wilhelm Meister with regard to form, theme and characterisation; an analysis of German philosophical elements in the novel; and Martin's presentation of social conditions in Germany in 1888-90, and their role in the novel as a whole. The examination of Henry Handel Richardson's work encompasses: biographical details; the genesis of Maurice Guest; differences between the reception of the novel in England and Germany; the genre to which the novel belongs and parallels with Künstlerromane; an analysis of Richardson's description of the physical, historical and intellectual milieu of Leipzig, and its role in the novel; and finally her integration of German social customs and the German language into the text. Use has been made of five primary sources which have not been used before in any detail with regard to these aspects of either author: additional material from the Mount Gambier Border Watch; The Hatbox Letters, the family history of the Martin and Clarke families; the German translation of Maurice Guest; German reviews of Maurice Guest; and the correspondence between Richardson and her French translator Paul Solanges. The key argument of this thesis is that the German influence on both form and content, in the case of An Australian Girl, and on style and content, in the case of Maurice Guest, is deep and various, and that these German elements have proved to be an impediment to a full understanding and appreciation of these novels for many Anglo-Saxon readers and reviewers. In the two novels Martin and Richardson provide pointers to Australia's earlier interaction with the wider world and display a level of sophistication which makes these works worthy of greater recognition than they currently enjoy.

>Magister Scientiae - MSc / Glucose oxidase (GOx) and horseradish peroxidase (HRP) are important enzymes for the development of amperometric enzyme linked immunosensors. The selectivity of each enzyme towards its analyte deepens its importance in determining the sensitivity of the resultant immunosensor. In designing immunosensors that have customized transducer surfaces, the incorporation with FAD and iron based enzymes ensures that electron kinetics remains optimal for electrochemical measurement. Various different immobilization strategies are used to produce response signals directly proportional to the concentration of analyte with minimal interferences. The combination of self-assembled monolayers and supramolecular chemistry affords stability and simplicity in immunosensor design. In this work, two electrochemical strategies for the detection of human chorionic gonadotropin(hCG) is presented. This involves the modification of a gold surface with a thiolated β-cyclodextrin epichlorohydrin polymer (βCDPSH) to form a supramolecular inclusion complex with ferrocene (Fc)-functionalised carboxymethyl cellulose polymer (CMC). Cyclic voltammetry indicated that ferrocene is in close proximity to the electrode surface due to the supramolecular complex formed with βCDPSH. Furthermore, strategy (a) for the detection of hCG used α-antihCG labelled (HRP) as reporter conjugate. Strategy (b) maintained the CMC bifunctionalised with Fc and recognition antibody for hCG hormone. However, the system was functionalised with a HRP enzyme and detection is done by using GOx reporter conjugates for in situ production of hydrogen peroxide. The reduction of H2O2 was used for the amperometric detection of hCG by applying a potential of 200 mV. The sensitivity and limit of detection of both strategies were calculated from calibration plots. For strategy (a) the LOD was found to be 3.7283 ng/mL corresponding to 33.56 mIU/mL and a sensitivity of 0.0914 nA ng-1 mL-1. The corresponding values for strategy (b) are 700 pg/mL (6.3 mIU/mL) and 0.94 nA ng-1 mL-1.

This thesis uses affect theory and studies of emotion to analyse literary representations of the everyday in fictional and non-fictional writing about Muslim migrant women in the UK from 1906 to 2012. Postcolonial literary studies tend to value exceptional events over mundane life, which causes possible issues of exoticism and a danger of homogenising distinct experiences. This thesis offers a theorisation of migration that foregrounds everyday experience through an engagement with theories of objects, bodies and space, as well as emotional experiences that are specific to migrant subjectivity. It analyses two groups of texts: early twentieth century travel writing by Atiya Fyzee, Shahbano Begum Maimoona Sultan and Zeyneb Hanoum, and contemporary literary texts by Yeshim Ternar, Farhana Sheikh, Monica Ali, Leila Aboulela, Elif Shafak and Fadia Faqir. The thesis is structured thematically into three sections, each section containing two chapters, one about travel writing and another about contemporary texts. In the first section, in order to examine how the texts negotiate foreignness in daily life, I consider hospitality theory, which describes how social power relations are based on roles of host and guest. In the second section, I argue that melancholia is an emotional experience endemic to migrancy. The texts demonstrate how this emotion is manifest communally as well as individually, which also shows the political potential of emotion. In the third section, I investigate how emotional processes of migration are described spatially in the texts. The findings of this research show that emotional knowledge is a major concern for migrant writers as a way of engaging with and critiquing the social and political climates of each text. This is produced through narrations about feeling in general and specific emotions, such as irritation or anxiety. Emotional experience is illustrated in conjunction with identities that are both fluid and intersectional, where gender and class converge with ethnicity and religion. The texts also show specifically affective styles of writing that concentrate on focalising women's intimate experiences through, for example, diary entries, bildungsroman or psychological realism. While the differing contexts reflect the particularities of each experience, there are sufficient similarities of narrative content and style to suggest that affective experience is a major concern for this body of literature. Overall, this thesis demonstrates the productive uses of affect theory as a critical stance for analysing postcolonial literature.

Online marketing communication provides some of the cutting edge tools that can be used by guest houses to communicate their product and service offerings. The purpose of this study was to determine which online marketing communication tools can be used, and are in fact currently used by guest houses in Pretoria East. The results obtained from this study can assist guest house owner/managers in Pretoria East with decision-making on the communication of off-line and online marketing communication tools to be included in their marketing strategy. Primary data was collected by means of a survey with guest house owners/managers in Pretoria East. Specific corpographic details of guest houses in Pretoria East were identified. Furthermore, the most frequently used off-line and online marketing communication tools were identified, as well as the number of guest houses that have websites and the content of these websites. The findings suggest that guest houses in Pretoria East have to invest more time and money in implementing certain online marketing communication tools, such as social networking and use of Search Engine Optimisation (SEO). By implementing online marketing communication tools like these, guest houses in Pretoria East could enhance customers‟ awareness of a guest house. This study verified the importance of electronic tools such as the Internet and Web as an integral part of an organisation‟s marketing strategy, specifically for small businesses in the service sector, such as guest houses. / Business Management / M. Com. (Business Management)

La synthèse et l'étude de rotaxanes et de porphyrines sont deux domaines particulièrement actifs de la recherche en chimie. Cependant, les composés combinant les propriétés intéressantes de ces deux types de structures sont plus rares. De nouveaux multi-rotaxanes à base de porphyrines, dont la préparation représente un défi synthétique, sont décrits dans cette thèse.Des porphyrines liées à deux ou quatre anneaux coordinants ont été synthétisées. Des rails moléculaires à deux chélates ont été enfilés dans les anneaux grâce à l'effet template du cuivre(I); l'introduction de bouchons a mené à la formation de rotaxanes. Dans le cas du bis-macrocycle porphyrinique, un [4]rotaxane a été obtenu. Des études de complexation hôte/invité avec des ligands azotés rigides ont montré que ce rotaxane est un récepteur moléculaire qui peut s'adapter à la taille du substrat invité en se "gonflant" ou en se "dégonflant".Dans le cas du tétra-macrocycle porphyrinique, la formation d'un [3]rotaxane d'architecture originale a étéobservée.La synthèse d'un nouveau bis-macrocycle plus rigide est en cours. Ce composé sera utilisé pour la construction d'un [4]rotaxane, qui pourrait montrer un caractère de presse moléculaire capable de modifier la conformation d'un substrat invité en le comprimant. / Rotaxanes and porphyrins are two particularly active fields of research in chemistry. However,molecules that combine the interesting properties of these types of structures are not so common. In this thesis we describe new porphyrin-based multi-rotaxanes, whose syntheses constitute interesting challenges.Porphyrins linked to two or four coordinating macrocycles were synthesised. The "gathering-andthreading" effect of copper(I) was used to thread molecular rods through the rings; the subsequent introduction of stoppers led to the formation of rotaxanes. In the case of the porphyrinic bis-macrocycle a [4]rotaxane was obtained. Host/guest complexation studies with rigid nitrogen ligands showed that the rotaxane behaves as a distensible molecular receptor that can adopt an "inflated" or "deflated" conformation and adjust its shape to the size of the guest. In the case of the porphyrinic tetra-macrocycle the formation of a [3]rotaxane of novel architecture was observed.The synthesis of a new, more rigid bis-macrocycle is in progress. This compound will be used for the construction of a [4]rotaxane that could act as a molecular press able to change the conformation of a guest substrate by compression.

L’orchestration des mouvements directionnels d’architectures supramoléculaires s’avère cruciale pour la préparation de machines moléculaires artificielles. Les oligomères d’amides aromatiques (i.e. foldamères) peuvent adopter des conformations stables capables de se complexer à des tiges moléculaires pour former des (pseudo)-rotaxanes. Un contrôle fin des cinétiques d’association et de dissociation de l’hélice autour de la tige permet à l’oligomère hélicoïdal de glisser le long de celle-ci sans dissociation. Des études RMN et cristallographiques ont montré que des tiges moléculaires possédant plusieurs sites de reconnaissance pour des hélices permettaient l’élaboration d’architectures supramoléculaires hélicoïdales chirales avec une haute-fidélité. Chaque station possédant une longueur et une chiralité définie peut induire la complexation de foldamères de taille et d’hélicité concordante. Le glissement directionnel d’une double hélice le long d’une tige possédant plusieurs stations a également été investigué. Insérer un espaceur encombrant (i.e. plus large que la cavité de l’hélice) sur le chemin du foldamère le force à se déplier et se replier pour atteindre le site le plus favorable thermodynamiquement. Un oligomère asymétrique montrant de hautes affinités et de fortes sélectivités pour des tiges asymétriques a été préparé. L’enfilement de cet oligomère sur des tiges asymétriques a été étudié. Des données cinétiques (RMN) indiquent que l’enfilement de celui-ci s’effectue de façon polarisée en fonction de la nature de la tige. / The directional motion orchestration of supramolecular architectures is crucial for the construction of artificial molecular machines. Aromatic amide oligomers (i.e. foldamers) can adopt stable helical conformations able to wind around dumbbell-like guests to form (pseudo)-rotaxanes. A fine control of the association-dissociation kinetics allows the oligomers to slide along the rods without dissociation. In this thesis, based on the segregation of the kinetics of association-dissociation and sliding, helical oligomer motions were orchestrated to form complex self-assemblies and to perform directional motion. NMR and crystallographic studies showed that multistation rod guests can template the formation of well-defined multi-helical supramolecular polymers with high fidelity. Each station possessing a defined length and chirality can induce the complexation of oligomers presenting matching length and chirality. Directional sliding of a double helical oligomer along linear multistation rod guests was investigated. Placing a bulky spacer on the rod prohibits the sliding process, forcing the oligomer to dissociate and reassociate onto the thermodynamically favored station. An asymmetrical oligomer was prepared showing highly selective binding toward asymmetrical rod guests. The threading of this oligomer onto linear asymmetrical guests was investigated. Kinetic data indicated that the threading orientation of this asymmetrical oligomer was polarized by its passage along guest molecules.

Master thesis aims to define entrepreneurship including lodging services, shows basic information about how to create entrepreneurship plan and afterwards making particular one about a concrete guest house. In the introduction you will find out the basic information about entrepreneurship and also about dividing accommodation services. Those information precede better understanding of the practical part, in which thesis aims to concrete opportunities of entrepreneurship based on a guest house in Karlovy Vary.

Les matériaux poreux silicatés ont été mis à profit pour encapsuler différents types de molécules, clusters ou nano-objets fonctionnels, donnant lieu à des nanocomposites hybrides organiques-inorganiques à propriétés physiques, chimiques ou biologiques remarquables. Élucider l'organisation structurale à l'échelle moléculaire de tels nanocomposites est indispensable pour l'analyse et la compréhension des propriétés macroscopiques qui en découlent. Ainsi, les techniques de diffusion totale associées à la fois à l'analyse Debye et la Fonction de Distribution de Paires (PDF) sont des méthodes de choix pour la caractérisation des propriétés structurales de matériaux hybrides nano-structurés. Le principal objectif de ce travail consiste à l'utilisation des approches basées sur la diffusion totale de rayons X pour l'analyse structurale complète de molécules photoactives confinées dans des matrices silicatées amorphes avec différentes tailles de pores, afin d'étudier l'influence de l'organisation structurale sur les propriétés optiques et d'explorer également les limites de ces approches d'analyse. Nous avons étudié deux systèmes photoactifs. L'analyse structurale du premier complexe confiné, Na2[Fe(CN)5NO].2H2O (SNP), a été entreprise par une approche multi-échelle combinant la RMN du solide et l'analyse PDF. Cette approche a permis l'identification de la nature des espèces incorporées, l'arrangement des cations et des anions ainsi que la distinction des différentes phases existantes : molécules isolées et nanoparticules. Les analyses Debye et PDF sur le deuxième composé étudié, [NdCl2(H2O)6]Cl, montrent que l'organisation structurale du complexe confiné est différente de celle du matériau massif cristallin. De plus, les cations Nd3+ changent de coordination de 8 à 9 durant le processus d'imprégnation et adoptent ainsi un arrangement structural très similaire à celui en solution aqueuse. Cette modification structurale est en accord avec le changement des propriétés luminescentes de ce complexe / Silica xerogels are versatile host materials for the inclusion of molecules, clusters, or nano-objects yielding host-guest compounds with unique physical, chemical or biological properties. The knowledge of the structural organization of the guest within the host is crucial for the understanding of its properties. Total scattering methods, based on Debye function analysis (DFA) and Pair Distribution Function (PDF), have become powerful tools for structural characterization of nanostructured hybrid materials. The aim of this work is to use the X-ray total scattering method to obtain structural information on photoactive molecules embedded into amorphous silica hosts with different pore sizes, to correlate their structure with the optical properties, and to explore the limitations of the chosen method. Two different photoactive complexes have been investigated. In the first example, the combined PDF and NMR study on Na2[Fe(CN)5NO].2H2O (SNP) embedded into silica matrices allows to extract the nature of the inserted species: quasi-free isolated molecules can be distinguished from nanoparticles and in the former case a model for the arrangement of cation-anion can be proposed from the PDF analysis. In the second example, a luminescent Nd3+ complex, the PDF and DFA analysis reveal that the structural organization of the embedded Nd3+ complexes is different from that of the crystalline material. Furthermore, the Nd3+ cations change the coordination from 8 to 9 during the wet-impregnation doping and adopt very similar structural arrangement as in aqueous solution, which is in agreement with the observed change in the luminescence properties

The demand for multifunctional nanocontainers possessing both recognition ability and responsive nature is increasing greatly because of their high potential in various biomedical applications. The engineering of such smart nanovesicles is useful to enhance the efficiency of many therapeutic and diagnostic tools that have the applicability in targeted drug delivery systems as well as designing sensing devices or conducting selective reactions as nanoreactors in the scope of nanobiotechnology. For this purpose, this study demonstrates the formation of multifunctional and stimuli-responsive polymersomes comprising various abilities including pH and light sensitivity as well as many reactive groups with sufficient accessibility to be used as smart and recognitive nanocontainers. The fabrication included several steps starting from the synthesis of azide and adamantane terminated block copolymers, which were then self-assembled to prepare the polymersomes with the corresponding functional groups for the subsequent post-conjugations at the vesicle periphery. The accessible and sufficiently reactive groups were quantitatively proven when UV and IR cleavable NVOC protected amino groups as well as β-cyclodextrin molecules were conjugated to the pre-formed polymersomes through click chemistry and strong host-guest complexations. The gained light responsivity with the aid of successful NVOC attachment enabled further selective photochemical reactions triggered either by UV or NIR light leading to liberated amine groups on the polymersome surface. Therein, these released amino groups were further conjugated with a model fluorescent compound as mimicking the attachment of biorecognition elements to see the direct picture of the applicability. To realize this concept in a more localized and selective way as well as to avoid the possible side effects of UV light, the NIR-light induced photochemical reactions and further dye coupling were performed when polymersomes were immobilized onto solid substrates. This fixation was achieved by adapting the host-guest chemistry into this part and conjugating the adamantane decorated polymersomes onto β-cyclodextrin coated substrates. Several investigations including adhesion behavior, pH sensitivity and mechanical properties of the established multifunctional polymersomes under liquid phase have been performed. It has been found that the polymersome shape is highly dependent on the attractive forces of the substrate and needs to be optimized to avoid the flattening of the vesicles. For these optimization steps, different conditions were investigated including the decrease of cyclodextrin amount and additional surface passivation with PEG molecules on the solid substrates. Besides, the calculated Young’s and bending modulus of the polymersome membrane from AFM measurements showed a robust but still flexible “breathable” membrane which is an important criterion for the applicability of these smart and stable vesicles. In addition, the hosting ability as well as diffusion limits and sufficient membrane permeability of the polymersomes were observed by encapsulating gold nanoparticles as a smart cargo and doxorubicin molecules as an anticancer drug. In conclusion, the established multifunctional polymersomes are highly versatile and thus present new opportunities in the design of targeted and selective recognition systems which is highly interesting for various applications including development of microsystem devices, design of chemo/biosensors, and also for conducting enhanced, combined therapy in the field of drug delivery.