Made available in DSpace on 2015-04-14T13:33:03Z (GMT). No. of bitstreams: 1 446510.pdf: 17222028 bytes, checksum: 91c5883f67ca4db80d44ff4a6092aa1c (MD5) Previous issue date: 2013-03-01 / Objectives: to investigate separately the therapeutic potential of neuropeptide NAP and the intra-arterial (IA) transplantation of human umbilical cord blood (HUCB) mononuclear cells in an animal model of neonatal hypoxia-ischemia (HI). Methods: male Wistar rats at postnatal day 7 were subjected to an HI model by permanent occlusion of right common carotid artery and systemic hypoxia (8% O2 for 2 h). The animals were randomly assigned to groups receiving an intraperitoneal injection of NAP (3 μg/g) immediately (0 h) and 24 h after HI. Other animals received HUCB mononuclear cells (1 x 106 or 1 x 107 cells/50 μL) into the left common carotid artery 24 h after HI insult by using the microneedle technique. Only for NAP study, brain DNA damage, lipid peroxidation and reduced glutathione (GSH) content were determined 48 h post-HI insult. The spatial version of the Morris water maze learning task and the stereological volume assessment were performed in adult animals that received both therapies. The accelerated rotarod test and cerebral and body weight determination were applied only for cellular therapy study. The HUCB mononuclear cells migration was monitored through nested-PCR analysis in the brain and systemic organs of transplanted-HI rats. Results: we observed that NAP prevented the acute HI-induced DNA and lipid membrane damage and also recovered the GSH levels in the injured hemisphere of the HI rat pups. Further, NAP was able to hinder impairments in learning and long-term spatial memory and to significantly reduce brain damage in adult animals previously subjected to neonatal HI. The IA transplantation in neonatal HI rat seemed to be a feasible and safe delivery route for HUCB mononuclear cells. The intra-arterially delivered cells hindered dose-dependently the learning and spatial memory impairments without brain damage recovery in adult rats. Additionally we further showed that HI insult or IA cell transplantation had no long-term impact in the body weight and motor function in rodents. The HUCB mononuclear cells could be promptly identified in the ischemic brain after IA transplantation and also in some peripheral organs until at least 30 days later. Conclusions: the viability and long-lasting beneficial effects of neuropeptide NAP and IA transplantation of HUCB mononuclear cells support its translational characteristic for neonatal HI management. Therefore, NAP and intracarotid delivery of cells became promising candidates for the treatment of HI-induced brain damage and life-long disabilities / Objetivos: investigar, separadamente, o potencial terap?utico do neuropept?deo NAP e do transplante intra-arterial de c?lulas mononucleares de sangue de cord?o umbilical humano (CMSCUH) em um modelo experimental de hip?xia-isquemia (HI) neonatal. M?todos: ratos Wistar machos com 7 dias de vida foram submetidos ao modelo de HI neonatal atrav?s da oclus?o permanente da art?ria car?tida comum direita e hip?xia sist?mica (8% O2 por 2 h). A administra??o do neuropept?deo NAP (3 μg/g, i.p.) ocorreu imediatamente (0 h) e 24 h ap?s a aplica??o do modelo. J? o transplante de CMSCUH (1 x 106 ou 1 x 107 c?lulas/50 μL) foi realizado 24 h p?s-HI atrav?s de t?cnica de microagulha na art?ria car?tida comum esquerda. Somente no estudo do NAP, ensaio cometa alcalino, peroxida??o lip?dica e n?veis de glutationa reduzida foram determinados no hipocampo e c?rtex cerebral 48 h p?s-HI. Para ambas as terapias empregaram-se a vers?o espacial do labirinto aqu?tico de Morris (LAM) e determina??o estereol?gica da volumetria hemisf?rica cerebral na fase adulta destes animais. Utilizou-se o teste do rotarod acelerado e pesagem corporal e cerebral dos animais adultos apenas no estudo do transplante intra-arterial de CMSCUH. A migra??o das CMSCUH foi monitorada atrav?s da t?cnica de nested-PCR no c?rebro e ?rg?os sist?micos. Resultados: o tratamento com NAP restabeleceu a integridade hipocampal e cortical do DNA e membranas lip?dicas, junto ao incremento do sistema glutationa. Al?m disso, o NAP impediu o desenvolvimento de d?ficits do aprendizado e da mem?ria espacial juntamente ? redu??o da atrofia cerebral em ratos adultos. O transplante intracarot?dico de CMSCHU em ratos neonatos HI demonstrou-se fact?vel e seguro. Este tratamento preveniu, de maneira dose-dependente, o desenvolvimento de preju?zo cognitivo de ratos adultos previamente expostos ? HI neonatal, sem influ?ncia sobre a atrofia cerebral. A fun??o motora e peso corporal de ratos adultos n?o sofreram influ?ncia da HI neonatal ou da administra??o intra-arterial de CMSCHU. As CMSCHU puderam ser detectadas tanto logo ap?s o transplante como tardiamente (30 dias) no c?rebro e ?rg?os sist?micos dos animais transplantados. Conclus?es: os efeitos ben?ficos e duradouros do neuropept?deo NAP e do transplante intra-arterial de CMSCHU, assim como sua viabilidade, fornecem evid?ncias de uma poss?vel translacionalidade destas duas terapias inovadoras para o tratamento da HI neonatal. Desta forma, o neuropept?deo NAP e a via intracarot?dica para o transplante de CMSCHU tornam-se candidatos potenciais para a preven??o do dano HI cerebral e decorrentes sequelas

Thesis (M.Phil.)--Hong Kong University of Science and Technology, 2008. / Includes bibliographical references (p. 81-85).

Thesis (Ph. D.)--University of Hong Kong, 2006. / Title proper from title frame. Also available in printed format.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2018-03-20T20:33:20Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andre_lima_ferreira.pdf: 1132174 bytes, checksum: c4e0d6855cc57f4423d6090c22f399cf (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2018-03-29T14:11:53Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andre_lima_ferreira.pdf: 1132174 bytes, checksum: c4e0d6855cc57f4423d6090c22f399cf (MD5) / Made available in DSpace on 2018-03-29T14:11:53Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) andre_lima_ferreira.pdf: 1132174 bytes, checksum: c4e0d6855cc57f4423d6090c22f399cf (MD5) Previous issue date: 2017 / Companhia Energ?tica de Minas Gerais (Cemig) / Banco do Nordeste / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (Capes) / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / Companhia de Desenvolvimento dos Vales do S?o Francisco e do Parna?ba (CODEVASF) / IMEVE Sa?de e Biotecnologia em Nutri??o Animal / Avaliou-se em dois experimentos a influ?ncia do efeito do simbi?tico, da levedura (Saccharomyces cerevisiae) e antibi?tico no cultivo de juvenis de til?pias do Nilo sobre as vari?veis de desempenho produtivo, resposta ao estresse por exposi??o ao ar e composi??o bromatol?gica da composi??o corp?rea dos peixes. Foram utilizados 250 e 625 juvenis de til?pia do Nilo (machos) no primeiro e segundo experimento, respectivamente e ambos tiveram dura??o de 56 dias. Os peixes foram distribu?dos em delineamento inteiramente casualizado, com cinco tratamentos, cinco repeti??es, totalizando 10 e 25 peixes aqu?rio-1, respectivamente. No primeiro experimento utilizando o simbi?tico, o antibi?tico e seu cons?rcio no cultivo de til?pias nil?ticas foram observadas diferen?as para os par?metros de peso final, ganho de peso, biomassa, consumo de ra??o, convers?o alimentar, sobreviv?ncia e mat?ria seca da composi??o corp?rea dos peixes, sendo o melhor resultado observado para os peixes cultivados com antibi?tico na ?gua e simbi?tico na ra??o (3A). A adi??o de simbi?tico na ra??o comercial no cultivo de juvenis de til?pia do Nilo mostrou-se eficaz para a melhoria do desempenho produtivo e quando suplementado em cons?rcio com antibi?tico teve seu efeito potencializado. No segundo experimento foi suplementada a levedura S. cerevisiae, o antibi?tico e seu cons?rcio para juvenis da mesma esp?cie, entretanto foram observadas diferen?as para os par?metros como consumo de ra??o, convers?o alimentar e sobreviv?ncia, sendo os melhores valores observados para as til?pias que alimentaram da ra??o com adi??o de antibi?tico (4B). Quanto ?s an?lises bromatol?gicas das composi??es corp?reas dos peixes cultivados com antibi?tico na ?gua e levedura na ra??o (3B) apresentaram maior valor para a mat?ria seca e extrato et?reo em rela??o ao tratamento controle. Ao analisar o cortisol plasm?tico ap?s as til?pias terem sido expostas ao ar, os resultados apresentaram similaridade e n?o diferiram entre si. O antibi?tico demonstrou-se eficaz para uma melhor convers?o alimentar e sobreviv?ncia de juvenis de til?pias do Nilo. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017. / The aim of the two experiments was to evaluate the influence of the symbiotic, the yeast (Saccharomyces cerevisiae) and the antibiotic in Nile tilapia juveniles on productive performance, hypoxia stress response and the bromatological composition of the fish carcass. Fish were cultured for 56 days, and 250 and 625 Nile tilapia juveniles (males) were used in the first and second experiments, respectively. Fish were distributed in a completely randomized design, with five treatments and five replications, totalizing 10 and 25 fish/aquarium-1, respectively. On the first experiment, utilizing the symbiotic, the antibiotic and its consociation in Nile tilapia juveniles were observed statistic difference between the treatments for the following parameters: final weight, weight gain, biomass, consumption, feed conversion, survivability, and dry matter of the carcass where the best result was observed in the water with antibiotic and symbiotic added to food (3A). The administration of symbiotic on the food for Nile tilapia juvenile was efficient to promote performance on Fish and can be potentialized by the consociation with the antibiotic. On the second experiment yeast (S. cerevisiae), antibiotic and its consociation were supplemented for the same juvenile species, however, statistic differences were observed between the treatments for the following parameters: consumption, feed conversion, and survivability where the best result observed in tilapias fed with the antibiotic in the diet (4B). The bromatological analysis of fish carcass of fish cultured in water added with antibiotics and yeast in the diet (3B) shown betters results for dry matter, and lipid analysis when compared to control. However, the cortisol after the air exposition has shown similarity for all treatments. Thus, the yeast S. cerevisiae is an alternative to the use of antibiotics as a growth promoter in Nile tilapia juveniles.

Made available in DSpace on 2014-12-17T15:32:30Z (GMT). No. of bitstreams: 1 DeborahPPI_TESE.pdf: 2601986 bytes, checksum: 4c8dd80ab38fbe004ce1877a1a160d8b (MD5) Previous issue date: 2012-02-24 / The aim of this study was determine whether an association exists in the gum tissue between the expression of markers of tissue hypoxia (HIF-1? and GLUT-1) with a marker of inflammatory activity (COX-2) and a marker of collagen degradation (EMMPRIN). Was performed immunohistochemistry with antibodies specific for these markers on 60 samples of gingival tissue divided into two groups: gums (n = 26) and gingivitis (n = 34) and expression was analyzed in the epithelial tissue and connective tissue . The reactivity epithelial for COX-2 was observed in only two cases as the HIF-1?, GLUT-1 and EMMPRIN was strongly expressed in the epithelial basal layer and the immunostaining was gradually decreased as the cells away from this layer, and negative in the region suprabasal in most specimens. In connective tissue, and HIF-1? EMMPRIN were strongly positive for most cases analyzed as GLUT-1 was negative in most cases. Immunostaining for COX-2 showed an association with gingival inflammatory infiltrate. The expression of EMMPRIN, HIF-1? and GLUT-1 in normal gums confirms the physiological role of these markers, however there was no association with tissue inflammation. Given the findings we can conclude that the inflammatory changes installed in frames of chronic gingivitis may not be sufficient to activate the factors of hypoxia to levels that can be quantified by immunohistochemical analysis, in addition, the findings are not conclusive in relationship to involvement of EMMPRIN in the secretion of MMPs to degrade collagen in the frames of gingivitis. We suggest the use of technical analysis and quantification of RNA of EMMPRIN and MMPs in order to determine whether collagen degradation observed in gingivitis suffers or not, significant influence of EMMPRIN for secretion and activation of MMPs / Este estudo pretendeu verificar se no tecido gengival normal e em quadros de gengivite existe associa??o entre a express?o dos marcadores de hip?xia tecidual (HIF-1? e GLUT-1) e os marcadores da atividade inflamat?ria (COX-2) e da degrada??o das fibras col?genas (EMMPRIN). Foi realizada a t?cnica imunohistoqu?mica com anticorpos espec?ficos para os referidos marcadores em 60 amostras de tecido gengival divididas em dois grupos: gengivas saud?veis (n=26) e inflamadas (n=34) e a express?o foi analisada no tecido epitelial e no tecido conjuntivo. A reatividade epitelial para a COX-2 foi observada em apenas dois casos enquanto o HIF-1?, GLUT-1 e EMMPRIN estavam fortemente expressos na camada basal do epit?lio e a imunomarca??o foi gradualmente diminuindo ? medida que as c?lulas afastavam-se desta camada, sendo negativa na regi?o suprabasal na maioria dos esp?cimes. No tecido conjuntivo, EMMPRIN e HIF-1? foram intensamente positivos para maioria dos casos analisados enquanto o GLUT-1 foi negativo na maioria dos casos. A imunomarca??o para a COX-2 revelou associa??o com o infiltrado inflamat?rio gengival. A express?o do EMMPRIN, HIF-1? e GLUT-1 em gengivas normais, confirma o papel fisiol?gico destes marcadores, entretanto n?o houve associa??o com a inflama??o tecidual. Diante dos achados pode-se concluir que as modifica??es inflamat?rias instaladas em quadros de gengivite cr?nica podem n?o ser suficientes para ativar os fatores de hip?xia a n?veis que possam ser quantificados atrav?s da an?lise imuno-histoqu?mica. Al?m disso, os achados n?o s?o conclusivos em rela??o ? participa??o do EMMPRIN na secre??o das MMPs para degrada??o do col?geno nos quadros de gengivite. Sugere-se a utiliza??o de t?cnicas de an?lise e quantifica??o de RNA do EMMPRIN e das MMPs com o intuito de verificar se a degrada??o do col?geno observada nos quadros de gengivite sofre, ou n?o, influ?ncia significativa do EMMPRIN para secre??o e ativa??o das MMPs

This thesis aims to shed new light on the Chinese character xia 侠 and the literature and history of the Republican Era (1912-1949) that revolves around it. Xia refers to either a concept (identifiable with kindness, altruism, righteousness, etc.) or to a person who practices this concept. Ever since its arrival in Chinese texts in the sixth century BC, it has created controversy for some and sympathy for others. In Modern China, xia became the central aspect of a literary genre that reached its zenith in production and consumption in the Republican Era, i.e. wuxia fiction 武侠小说, which can be translated as “using martial arts (wu 武) to obtain xia”. The concept of xia was an integral part of presumably the most widespread literary genre of the time, but why were Republican-era readers so interested in it? Why did they relate to xia and what do the themes of these novels say about the chaotic Republican Era? To answer these questions, this thesis presents a case study of a wuxia pentalogy written by Wang Dulu at the end of the Republican Era and attempts to identify the topics and aspects most reflective of that historical period, showing that, despite the heavy criticism of intellectuals of that time, these “easy” popular novels contain innovative and modern aspects and can become today of great historical importance. The thesis starts with two literature reviews. The first determines that the term xia has not received enough scholarly attention, calling for a reassessment. The second literature review focuses on Republican Era wuxia fiction, showing how there is a gap in scholarship on this period. This is followed by a discussion of the methodology used for the analysis of the case study on Wang Dulu’s Crane-Iron Series written in Qingdao (1938-1944), presented in the final three chapters of the thesis. Chapter one analyses the origins of the term xia in texts from the Warring States Period (475-221 BC) and Han Dynasty (206 BC-AD 220), presenting new interpretations for a more comprehensive understanding of the term. Chapter two gives a historical overview of xia¬-related literature and addresses the historical reasons for the changes that xia underwent throughout Chinese history. Chapter three includes a historiography of the Republican Era in combination with the life of the author Wang Dulu and identifies the aspects of the author’s life that will become important in the textual analysis in the chapters to follow. Chapter four focuses on xia in the Crane-Iron Series. After having collected the terms and identified the semantic spheres that include the Chinese character xia, the chapter demonstrates how the story of one of the series’ protagonist can be seen as an personification of Republican-era China, proving the historical dimension and value of these novels. Chapter five analyses yi 义 (righteousness) and represents the virtuous aspect of xia, concluding that, according to Wang Dulu, for the concept of xia, virtue is more important than being trained in martial arts (wu). Chapter six focuses on the literary figure of the baobiao 保镖 (protector) and is seen as the commercialisation of martial arts not necessarily linked to xia, showing how entrepreneurship and violence were characteristics of the time.

Since the federal funds rate reached the zero lower bound in late 2008, economists have been struggling to adapt their models to a long-term zero rate. Wu and Xia built upon previous research by Fischer Black to create a model for how the federal funds rate behaves during the ZLB period. In their model, the rate actually dips into the negative digits, which the actual federal funds rate does not do. The logic behind the model is that a negative shadow rate is a much better indicator of true economic conditions while the current zero rate merely masks the actual economic reality. It is also easier to use the shadow rate for trend analysis purposes, since the shadow rate is flexible and changes while the federal funds rate remains artificially fixed at zero. Thus, this paper seeks to provide a comparison between the Shadow Rate, as defined by Wu and Xia, and how three key banking variables (leverage, profitability, and non-performing loans to total loans) react in response to the shadow rate, along with three control variables: real GDP growth, inflation, and the current account to GDP ratio. Regression will also be used to determine how three key borrower variables (S&P 500 Index, Credibility Consumer Distress Index, and the ratio of nonfinancial corporate business debt securities to total assets) interact with the shadow rate and the three control variables previously mentioned.

本論文重點在研究徐霞客遊記之文學。全一冊，除前言外，凡七章，約十萬言各章大 要如下： 第一章：徐霞客其人。簡介徐霞客之家世、生平、性情、交遊等，以認識其人。 第二章：徐霞客遊記概述。首敘遊記之成書、整理及刊刻，次敘霞客之旅遊概況與遊 跡，三敘遊記之內容、形式等，期能對遊記本身有一大體之瞭解。 第三章：徐霞客所處時代之文學背景與所受影響。此章擬自晚明文風、晚明文人之寫 作態度、小品文之興起三方面，見霞客寫作之背景。 第四章：徐霞客之寫作技巧分析。本章從構語造句，修辭用字，譬喻三方面，探論其 寫作技巧。 第五章：徐霞客遊記文學所常見之主題試探。由山之屬、岩溶洞穴、天象氣候、人物 、植物等，見其文學風貌。 第六章：往霞客遊記與我國山水名家比較。茲舉酈道元、柳宗元、袁宏道三家之山水 文學與霞客之遊記比較，以見各家於山水文學寫作之異同。 第七章：結論。論霞客遊記文學之評價及其成就。

This thesis deals with analysis of development of Chinese views on the origins of Chinese culture and statehood in view of archaeological research between the years 1940 and 1980. The analysis is based on information provided by popularizing media, specifically Chinese newspapers and high school textbooks, which are paired with archaeological overview from specialized publications written in English, Czech and Chinese language. Thesis consists of three parts. Firstly, it outlines development in archeology during four decades and the impact of nonacademic influence on scientific work. It serves as theoretical reference frame for the following analysis of Chinese sources. The second chapter deals with reflection of archaeological research in articles of the People's Daily newspapers. How are the archaeological findings valued by society, how does state ideology impacts on their research and it is related to that what are the geographical tendencies. Terminology and biased manner of expression in articles are also included to analysis. The third chapter consists of the study of views on the history of Chinese antiquity in textbooks. In which ways were textological historiography and outcome of archaeological research combined, including factual chronology of historical events and geographical location...

洪若震. / 論文(哲學碩士) -- 香港中文大學硏究院中國語言及文學學部, 1998. / 參考文獻: leaves 261-271. / 中英文摘要. / Hong Ruozhen. / Chapter 第一章 --- 引言 --- p.1 / Chapter 第二章 --- 《古文四聲韻》的作者與成書背景 / Chapter 第一節 --- 《古文四聲韻》的作者 --- p.4 / Chapter 第二節 --- 《古文四聲韻》的成書背景 --- p.9 / 附錄：夏竦年表 --- p.19 / Chapter 第三章 --- 《古文四聲韻》的流傳和版本研究 / Chapter 第一節 --- 《古文四聲韻》的流傳 --- p.21 / Chapter 第二節 --- 《古文四聲韻》的版本 --- p.29 / Chapter 第三節 --- 版本比較所見問題 --- p.39 / 附錄一：《古文四聲韻》清本、宋本、僧本比較表 --- p.46 / 附錄二：上聲韻目比較表 --- p.93 / 附錄三：《古文四聲韻》僧本、宋/清本韻字字序比較表 --- p.95 / 附錄四：汪本《古文四聲韻》、《說略》引書比較表 --- p.101 / 附錄五：《古文四聲韻》宋刻配抄本配抄部分與汪本、《四庫》 本比較表 --- p.102 / Chapter 第四章 --- 《古文四聲韻》與《汗簡》 / Chapter 第一節 --- 《古文四聲韻》與《汗簡》的關係 --- p.103 / Chapter 第二節 --- 《汗簡》、《古文四聲韻》互證 --- p.112 / 附圖一 --- p.118 / 附圖二 --- p.119 / 附圖三 --- p.120 / 附錄一：《古文四聲韻》、《汗簡》引書比較表 --- p.121 / 附錄二：《古文四聲韻》、《汗簡》比較表 --- p.125 / Chapter 第五章 --- 《古文四聲韻»與《廣韻》、《集韻》 / Chapter 第一節 --- 《古文四聲韻》與《廣韻》 --- p.165 / Chapter 第二節 --- 《古文四聲韻》與《集韻》 --- p.184 / 附圖一 --- p.191 / 附圖二 --- p.192 / 附圖三 --- p.193 / 附圖四 --- p.194 / 附圖五 --- p.195 / 附錄一：唐時韻書部次先後表 --- p.196 / 附錄二：〈古文四聲韻〉、《集韻》所收字字形比較表 --- p.199 / Chapter 第六章 --- 《古文四聲韻》的評價和影響 / Chapter 第一節 --- 《古文四聲韻》的評價問題 --- p.239 / Chapter 第二節 --- 《古文四聲韻》的影響 --- p.249 / 附圖一 --- p.256 / 附圓二 --- p.257 / Chapter 第七章 --- 結論 --- p.258 / 參考書目 --- p.261