Made available in DSpace on 2014-06-11T19:27:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-04Bitstream added on 2014-06-13T20:56:09Z : No. of bitstreams: 1 rodrigues_to_me_araca.pdf: 1242136 bytes, checksum: f935eebe57d980d63dfba1575e1ab773 (MD5) / Considerando que a práxis docente na educação básica abrange educação em saúde, esta pesquisa teve como objetivo avaliar o resultado de ações de educação em saúde oferecidas por meio de um curso de formação continuada, com atividades a distância por meio do Ambiente Virtual de Aprendizagem TelEduc e encontros presenciais, para docentes da rede municipal de Ensino Fundamental I de Araçatuba-SP. O curso foi organizado em duas fases, sendo a Fase I subdivida em módulos de temas como ambientação tecnológica, doenças transmitidas por vetores, doenças transmitidas por cães e gatos, higiene de alimentos, posse responsável e educação em saúde na escola e a Fase II para elaboração e aplicação de projetos educativos. Conhecimentos específicos dos docentes sobre dengue, febre amarela e leishmaniose, foram comparados antes e após o curso, observando-se um crescimento estatisticamente significante no conhecimento sobre estas doenças (p<0,0001) com destaque para as questões relativas ao agente etiológico das doenças, sintomas no homem e medidas de prevenção. A prática de aplicação do projeto educativo teve como critério as ações de multiplicação feitas pelo público-alvo. A experiência de multiplicação dos professores envolveu escolares, que diagnosticaram os problemas por meio de mapa falante e promoveram ações educativas no bairro, destacando-se, dentre outras: peças teatrais, atividades artísticas, mutirão de limpeza e elaboração e distribuição de panfletos em passeata. Conclui-se que a formação continuada de docentes, por meio de cursos semipresenciais, proporciona conhecimentos, impulsiona-os a motivar os educandos a adotar medidas de controle de vetores, cuidar do meio ambiente e sensibilizar a comunidade, colaborando para o controle de doenças / Considering that the educational praxis on basic education level also includes health education, our purpose was to evaluate the results of an online semi-present continued formation program, directed to teachers from Fundamental I Education level in Araçatuba-SP. The course was organized in two parts, with the Part I divided in themes as technological environment, vector borne diseases, diseases transmitted by dogs and cats, food hygiene, responsible ownership and health education in school and Part II reserved for elaboration and application of educative projects. The specific knowledge about dengue, yellow fever and leishmaniasis was compared before and after the attendance to a course with distance and present classes. Tests showed a statistically significant positive inversion in the knowledge about the studied diseases (p<0.0001) after the course, with emphasis to questions related to diseases etiological agents, symptoms in man and prevention measures. The application of practical educative projects was evaluated by multiplicative actions involving target public. The experience of knowledge multiplication included teachers and students which detected the problems by using talking maps and promoted education actions in their neighborhood, specially mimic plays artistic activities, group cleaning activities and elaboration and distribution of educational material in public demonstrations. We conclude that continuing education using semi-present courses, provides new knowledge to the teachers, impelling them to motivate their students to adopt vectors control measures and environmental cares and to sensitive the community, collaborating this way to control diseases

Orientador: Maurício Lacerda Nogueira / Banca: Carlos Eduardo Calzavara Silva / Banca: Fátima Pereira de Souza / Resumo: O Vírus da Febre amarela (YFV) causa doença com considerável morbidade e mortalidade nas regiões tropicais. Diversos vírus possuem estratégias para a alteração dos processos celulares. Mecanismos de splicing celulares são essenciais para diversificar a expressão dos genes e podem aumentar seu potencial de gerar proteínas. A replicação de YFV e as interações entre proteínas virais e celulares não são totalmente conhecidas. A proteína celular hSlu7 possui sinal de localização nuclear e tem um papel importante nas reações catalíticas do segundo passo do splicing. Estudos demonstram que sob infecção de YFV hSlu7 transloca para o citoplasma. A translocação de proteínas entre o citoplasma e o núcleo pode representar um mecanismo viral da regulação da expressão gênica. Este estudo teve como objetivo a caracterização da interação entre a proteína hSlu7 e NS5 viral, bem como estudar os efeitos de interações sobre os mecanismos de splicing alternativo após a infecção de YFV. Para identificar interação de NS5 de YFV com hSlu7 foi realizado ensaio de co-imunoprecipitação. Para verificar alteração de splicing celular foram utilizados replicons pEGFP-ADAR, pI12-IL7R, pEFGP-FGFR2, bem como a alteração de isoformas de XBP-1 endógenos. Os resultados indicam que NS5 de YFV interage com proteína hSlu7 e que sua interação pode influenciar no metabolismo RNA celular. YFV demonstrou exercer modulação no splicing celular, a avaliação de replicons sugerem que em splicing dependente de hSlu7, bem como a independente ocorre uma regulação viral atuando sobre sítios de splicing fraco e que a interação hSlu7-NS5 pode alterar direta e indiretamente a regulação trans-acting / Abstract: Yellow fever virus (YFV) causes disease with significant morbidity and mortality in tropical regions. Several viral strategies are avail for recruitment and alteration of the biochemical cellular processes. Cellular splicing mechanisms are essential to diversify the gene expression and increase it's proteomic potential. Replication of YFV and the interactions between viral and cellular proteins are unknown. The cellular protein hSlu7 has an nuclear localization and an important role in the second catalytic reaction step of the alternative splicing. In our study group demonstrated that under YFV infection hSlu7 translocates to the cytoplasm. The translocation of proteins between nucleus and cytoplasm may represent a viral mechanism of cellular gene expression regulation, interference in the protein availability of the alternative splicing and viral replication control. This study aimed to characterize the interaction between the viral protein hSlu7 and NS5, as well as studying the effects of interactions on the mechanisms of alternative splicing after YFV infection. To identify interaction with YFV NS5 hSlu7 was conducted co-immunoprecipitation assay. To verify changes in cellular splicing replicons were used pEGFP-ADAR, PI12-IL7R, pEFGP-FGFR2 as well as the change of isoforms of XBP-1 endogenous.The results indicated that YFV NS5 protein interacts with hSlu7 and that their interaction may influence cellular metabolism RNA. YFV perform modulation on cellular splicing, the evaluation of replicons suggest that in hSlu7 splicing dependent and independent regulation occurs viral acting on weak splice sites and that the interaction hSlu7-NS5 can change directly or indirectly to regulate trans- acting / Mestre

A dengue é a mais importante arbovirose no mundo; aproximadamente 50 milhões de infecções ocorrem anualmente acarretando 500.000 casos de dengue hemorrágica e 22.000 mortes. A febre amarela é uma doença hemorrágica viral com elevada mortalidade que é transmitida por mosquitos. Vacinas eficazes contra a febre amarela já estão disponíveis há quase 70 anos e são responsáveis por uma redução significativa de ocorrências da doença no mundo, no entanto, cerca de 200.000 casos de febre amarela ainda ocorrem anualmente, principalmente na África. Dessa forma, o desenvolvimento de fármacos antivirais contra essas viroses é uma prioridade de saúde pública. Os produtos naturais sejam de origem vegetal ou animal, possuem uma extensa diversidade química, sendo uma fonte inesgotável de compostos com promissoras atividades biológicas. No Brasil, é grande a incidência de animais venenosos ou peçonhentos, tais como serpentes, sapos e escorpiões. Os venenos desses animais são fontes de diversas substâncias químicas que ainda não possuem a sua atividade biológica e farmacológica completamente estudada. Neste trabalho avaliamos a potencial ação antiviral de peçonhas de serpentes (Crotalus durissus terrificus, Bothrops jararacussu, Bothrops jararaca, Bothrops pirajai, Bothrops moojeni, Bothrops brasili e Bothrops fonseca) e escorpião (Tityus serrulatus) contra os virus da febre amarela e dengue usando diferentes estratégias metodológicas (pré-tratamento, pós-tratamento, virucida, adsorção e internalização). Primeiramente realizamos um screening com as peçonhas brutas, observando que a peçonha de Crotalus durissus terrificus inibiu a replicação viral apresentando os maiores índices de seletividade (IS). Crotoxina, crotamina, crotapotina, convulxina, giroxina, PLA2-CB e PLA2-IC, isoladas de Crotalus durissus terrificus, foram então testadas nas diferentes estratégias metodológicas contra os vírus dengue e febre amarela. Foi possível verificar que crotoxina, PLA2-CB e PLA2-IC inibiram a replicação viral com altos índices de seletividade (IS). A ação verificada ocorreu na fase inicial do ciclo de replicação viral (pré-tratamento, virucida, adsorção). A ação antiviral verificada neste estudo foi atribuida a ação da PLA2, visto que a crotoxina é um complexo protéico composto pela crotapotina e pela PLA2-CB. Posteriormente avaliamos uma fosfolipase sem atividade catalítica isolada de Bothrops jararacussu, a BthTX-I. Essa fosfolipase apresentou baixa inibição da replicação viral, sugerindo que a atividade catalítica da fosfolipase é importante, mas possivelmente não a única responsável pela ação antiviral. Os resultados obtidos permitem sugerir também que as fosfolipases apresentam ação tanto sobre a partícula viral quanto sobre receptores celulares, o que justifica os altos índices de seletividade observados. / Dengue is the most important arbovirus disease in the world; nearly 50 million infections occur annually resulting in 500,000 cases of DHF and 22,000 deaths. Yellow fever is a viral haemorrhagic fever with high mortality that is transmitted by mosquitoes. Effective vaccines against yellow fever have been available for almost 70 years and are responsible for a significant reduction of the disease worldwide. However, about 200,000 cases of yellow fever still occur annually, mainly in Africa. Thus, the development of antiviral drugs against these viruses is a public health priority. Natural products of plant or animal origin have an extensive chemical diversity, and an inexhaustible source of compounds with promising biological activities. In Brazil, there is a high incidence of poisonous or venomous animals such as snakes, frogs and scorpions occur. The venoms of these animals are a source of several chemicals that does not possess biological and pharmacological activity completely studied. In this study, we assess the potential antiviral action of snake venom (Crotalus durissus terrificus, Bothrops jararacussu, Bothrops jararaca, Bothrops pirajai, Bothrops moojeni, Bothrops brasili and Bothrops fonseca) and Scorpion (Tityus serrulatus) against yellow fever and dengue viruses using different methodological strategies (pre-treatment, post-treatment, virucidal, adsorption and internalization). First, we performed a screening with the crude venoms, founding that the venom of Crotalus durissus terrificus inhibited viral replication showing the highest selectivity index (SI). Crotoxin crotamin, crotapotin, convulxin, gyroxin, PLA2-CB and PLA2-IC isolated from Crotalus durissus terrificus, were then tested in the different methodological strategies against dengue and yellow fever viruses. We found that crotoxin, PLA2-CB and PLA2-IC inhibited viral replication with high SI. The action of these compounds against the virus was at the first steps of the replication cycle (pre-treatment, virucidal, adsorption). The antiviral action observed in this study was attributed to the action of PLA2, since crotoxin is a protein complex composed of crotapotin and PLA2-CB. Afterwards, we evaluated a phospholipase without catalytic activity isolated from Bothrops jararacussu, the BthTX-I. This phospholipase showed low inhibition of viral replication, showing that the catalytic activity of phospholipase is important, but perhaps not the only one responsible for the antiviral action. Our results also suggest that phospholipases have action on the viral particle and on cell receptors, which explains the high levels of selectivity observed.

The maturation of the public sphere in Argentina during the late nineteenth and early twentiethcenturies was a critical element in the nation-building process and the overall development ofthe modern state. Within the context of this evolution, the discourse of disease generatedintense debates that subsequently influenced policies that transformed the public spaces ofBuenos Aires and facilitated state intervention within the private domains of the city’sinhabitants. Under the banner of hygiene and public health, municipal officials thusEuropeanized the nation’s capital through the construction of parks and plazas and likewiseutilized the press to garner support for the initiatives that would remedy the unsanitaryconditions and practices of the city. Despite promises to the contrary, the improvements to thepublic spaces of Buenos Aires primarily benefited the porteño elite while the efforts to rootout disease often targeted working-class neighborhoods. The model that reformed the publicspace of Buenos Aires, including its socially differentiated application of aesthetic order andpublic health policies, was ultimately employed throughout the Argentine Republic as theconsolidated political elite rolled out its national program of material and social development.

No description available.

Blood-feeding mosquitoes, by transmitting parasites and viruses to their hosts, kill several hundred thousand people every year. Mosquito populations are currently developing raising levels of insecticide resistance, and there is a need for a better understanding of their behavior so that new control solutions can be imagined, and existing ones can be improved. There has been a vast number of studies examining the host seeking behavior of mosquitoes, however there is a lack of knowledge concerning how mosquitoes are evading the threats their hosts pose via their defensive behaviors. Female mosquitoes are indeed in this unique position where their fitness and reproduction depend on them being able to locate hosts as well as evade them. In order to do this, they rely on sensory cues that they must be able to continuously re-evaluate during host tracking to potentially decide to quickly escape at any point during these interactions. Host seeking is mediated by multiple sensory modalities such as vision, olfaction, and thermosensation. However, it is not clear whether mosquitoes may also be using some of these same cues to identify that their host is turning into a threat. Focusing solely on visual cues in the context of escape behavior, we used a looming stimulus to elicit escape responses from the Yellow Fever mosquito, Aedes aegypti. A virtual reality environment was adapted from previous work, to display the looming stimulus while the mosquito is in a variety of conditions (i.e., landed, in free or tethered flight). Results from these experiments allowed us to characterize the escape responses of mosquitoes, by determining the angles and distances to the stimuli that will most likely trigger an avoidance response. / Master of Science in Life Sciences / Mosquitoes are the deadliest animals in the world because of the several diseases they can transmit. Females are the only ones that bite, since they require a blood meal in order to produce offspring. The task of obtaining this blood meal from a mobile, and defensive host can be extremely dangerous. The females must find a host, approach it, land, feed, and flee without being killed so they can reproduce. At any point, the host might detect the mosquito and try to kill it, typically by swatting at it. For this reason, mosquitoes have evolved the ability to navigate in close proximity to the host, while assessing their level of defensiveness and avoiding these threats. Insects, in general, are well known to display escape behaviors in response to visual, predator-like, looming stimuli. However, in spite of great epidemiological importance, very little is known about the mechanisms that allow mosquitoes to evade their predators, as well as swatting from their hosts. Mosquitoes use visual, olfactory, and thermal cues to track their hosts but what kinds of sensory cues are being used to avoid threats? As a first step towards bridging this knowledge gap, we analyzed the behavioral responses of the Yellow Fever mosquito, Aedes aegypti, to looming visual stimuli. For this, we used a virtual-reality environment designed for mosquitoes, where we displayed looming squares to mosquitoes in a variety of conditions (for example: landed, in flight). Results from these experiments allowed us to characterize the escape responses of mosquitoes, by determining the angles and distances to the stimuli that will most likely trigger an avoidance response. Ultimately, better understanding mosquito vision in the context of their escape behavior, can help us improve the design of control tools, such as traps, to increase their efficiency.

Situating the 1878 yellow fever epidemic in Memphis's long history shows how concern over Memphis's national reputation influenced how city leaders dealt with crisis. Throughout its history, Memphis government officials and business leaders promoted Memphis as a good city to do business, free from disease and racial strife. Despite their best efforts, they could not deny explosive incidents of racially-based violence or disease outbreaks. Instead, they tried to mitigate negative repercussions on the local economy during times of crisis. When the 1878 yellow fever epidemic struck, the Citizen's Relief Committee, the impromptu government formed by business leaders after outbreak, promoted Memphis as a functioning white city that was operating the best it could under terrible circumstances so the city could resume normal economic activity once the fever passed. This became the dominant narrative, repeated by newspapers across the country in 1878 and historians today. This narrative is problematic because it ignores black Memphians, who composed of 80% of the city's population after outbreak. Instead of recognizing black Memphians participation in relief activities, they promoted stories in the media about lazy or riotous African Americans to justify denying sufficient aid to the black community. Catholics had better luck earning the gratitude of Memphis's leaders. They worked with the white government and charities as nurses and fundraisers, and earned a glowing reputation in national newspapers. The inclusion of African Americans and Catholics in this thesis tells a more complete story and challenges white Memphians' carefully cultivated narrative. / Master of Arts

Submitted by Priscila Nascimento (pnascimento@icict.fiocruz.br) on 2012-11-16T11:43:38Z No. of bitstreams: 1 mauro-franca-da-silva.pdf: 1113117 bytes, checksum: 4721a993f32ab60140b4d0cf8c0f704f (MD5) / Made available in DSpace on 2012-11-16T11:43:39Z (GMT). No. of bitstreams: 1 mauro-franca-da-silva.pdf: 1113117 bytes, checksum: 4721a993f32ab60140b4d0cf8c0f704f (MD5) Previous issue date: 2007 / Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. / O vírus atenuado da febre amarela, subcepa 17DD, é utilizado por Bio-Manguinhos para a produção da vacina contra a febre amarela. Esta vacina tem sido utilizada para a imunização humana com um excelente histórico de eficácia e segurança. Entretanto, nos últimos anos, devido à ocorrência de alguns casos de eventos adversos associados ao vírus vacinal cepa 17D e subcepa 17DD, apontou-se a necessidade de desenvolvimento de uma vacina inativada. Para a implementação desta nova vacina torna-se necessário o desenvolvimento de métodos de quantificação de antígenos virais. Diferentes metodologias de quantificação podem ser utilizadas na produção de vacinas inativadas, sendo as mais comuns o teste imunoenzimático (ELISA) e o teste de dose-resposta. O presente estudo teve como objetivo o estabelecimento de um ELISA visando à detecção do antígeno do vírus da febre amarela inativado. Para este propósito, foram obtidos estoques de partículas virais da subcepa 17DD, a partir de culturas de células Vero, os quais foram purificados e quantificados por métodos bioquímicos e virológicos clássicos, respectivamente. Para o desenvolvimento do teste utilizamos diferentes anticorpos como capturana fase sólida. Os resultados obtidos para os testes utilizando o anticorpo 2D12 como captura mostraram um limite de detecção do antígeno no ELISA foi de 2,21 log 10 PFU/0,1mL e (1,55 µg/0,1mL). A partir deste valor, foi estabelecido um controle positivo contendo o vírus 17DD atenuado com título de 3,06 log10 PFU/mL e (29µg/0,1mL). Os resultados mostram, também, que o ELISA foi capaz de detectar o vírus 17DD inativado por formaldeído até a diluição 1:16 (52,9 µg/0,1mL). Baseado nos resultados obtidos acredita-se que o desenvolvimento de um teste de ELISA para detecção e quantificação do antígeno 17DD possa representar umimportante avanço tecnológico no controle da produção de uma vacina inativada contraa febre amarela. / The attenuated 17DD substrain of yellow fever virus is used in Bio-Manguinhos for yellow fever vaccine production. This vaccine has been used for human immunization with an excellent history of efficacy and safety. However, in the latest years, the occurrence of adverse events associated with 17D and 17DD substrain pointed to the necessity of developing technologies for the production of an inactivated vaccine. The implementation of this new vaccine will require methods for antigen quantification. Different methodologies of quantification can be used, being the most commonlyused the Enzyme-Linked Immunosorbent Assay (ELISA) and dose response test.The aim of this study was the establishment of an ELISA for the detection of inactivated yellow fever virus antigen. For this purpose, 17DD virus was obtained from Vero cell cultures, purified and quantified by biochemical and virological classical methods, respectively. The results showed that ELISA test using the 2D12 capture antibody presented a limit of 2,21 log10PFU/0.1mL of viral titer and (1,55 µg viral protein/0.1mL). Based on this value, a positive control was established which contained the attenuated 17DD substrain of yellow fever virus with a titer of 2,95log 10 PFU/mL and (29 µg/0.1mL). The results also showed that the ELISA was able to detect 17DD virus inactivated by formaldehyde up the dilution 1:16 (52,9 µg protein/0,1mL). The development of an ELISA test for the detection and quantification of 17DD antigen can represent an important step in the production control of the inactivated vaccine against of yellow fever.

The emergence of germ theory during the nineteenth century transformed Western medicine. By the 1870s, public health officials in the American South used germ theory to promote sanitation efforts to control public health crises, such as yellow fever epidemics. Before the discovery of mosquito transmission of yellow fever, physicians of the late nineteenth century believed the disease was spread by a highly contagious germ. Prominent medical practitioners of New Orleans, such as Confederate Army veteran Dr. Joseph Jones, used available scientific knowledge and investigation to attempt to control yellow fever during the Reconstruction period, a period rife with political and racial tension in New Orleans. This paper will analyze nineteenth century Southern medicine through the work of Dr. Joseph Jones and will argue that despite the use of cutting edge scientific methods of the era, the political challenges of the Reconstruction period shaded the public health policies in New Orleans.

A Febre Amarela (FA) é uma doença viral infecciosa, não contagiosa que pode se manifestar desde um quadro febril até a forma clássica íctero-hemorrágica, cujo agente etiológico é o vírus da febre amarela (VFA). A doença tem avançando para as regiões Sudeste e Sul com um aumento de 4 vezes no número de vítimas fatais. O presente trabalho teve como objetivos avaliar a possível circulação do vírus da febre amarela silvestre, através da detecção de anticorpos IgM por meio da técnica de MAC-ELISA, e anticorpos IgG, pelas técnicas de Inibição da hemaglutinação e Neutralização; detecção do genoma viral utilizando a técnica de RT-PCR no soro de indivíduos assintomáticos; bem como avaliar a proteção vacinal, por meio da técnica de neutralização em soros de indivíduos das zonas rurais do município de Jacupiranga e Teodoro Sampaio. Um total de 238 soros foram coletados, 152 (Jacupiranga) e 86 (Teodoro Sampaio). Foram detectados um total 15,9% (38/238) de anticorpos IH contra o vírus selvagem e/ou vacinal. Destes 13,16% (5/38) anticorpos IH foram provenientes dos soros de Jacupiranga e 86,84 (33/38) de Teodoro Sampaio. Anticorpos neutralizantes foram detectados em 34% (13N/38IH) dos IH reativos, destes 15,38% (2/13) foram oriundos deJacupiranga e 84,62% (11/13). A detecçção de anticorpos neutralizantes nos indivíduos de Jacupiranga levantam a necessidade de pesquisas futuras na busca do vírus da febre amarela nos seus reservatórios selvagens e vetores. Não houve a detecção do genoma viral por RTPCR nas duas populações, bem como a detecção de anticorpos IgM específicos, por meio das reações de MACELISA, o que evidencia a não circulação recente deste vírus na população estudada. O grau de imunizaçao na população de Teodoro Sampaio, com histórico de vacinação, por meio das reações de IH e Neutralização foi baixo, uma vez que somente 42,30% da população apresentou anticorpos inibidores da hemaglutinação e o grau de proteção pela reação de neutralização foi de apenas 33,33%. Este trabalho aponta não só para a necessidade de monitoramento pós-vacinal em áreas endêmicas de febre amarela, com indicação de vacinação, como também a necessidade de estudos epidemiológicos, uma vez que se detectou reação monotípica no teste do IH, além de anticorpos neutralizantes para o vírus selvagem da febre amarela em um indivíduo residente em município de risco para a febre amarela. / Yellow fever (YF) is an non-contagious infection disease. The clinical signs can be a nonspecific fever or the classical ictero-hemorrhagic form as a result of the Yellow Fever virus (YFV) infection. Considering the recent spread of the disease to the South and Southeast of Brazilian regions and the increased number of fatal cases, the aim of this study was to evaluate the possible circulation of the YFV, using the IgM enzyme-linked immnunosorbent assay (MACELISA) and Reverse transcriptase polymerase chain reaction (RT-PCR), as well as, to conduct a serological inquire and evaluate the protective vaccine response, by the hemagglutination inhibition test (HI) and serum neutralization test (SN), in the rural population of Jacupiranga and Teodoro Sampaio, located in the southern region of Brazil. A total of 238 serum samples were tested, 152 from Jacupirang and 86 from Teodoro Sampaio. Of the serum collected, 15,9% (38/238) were positive by HI, using wild and vaccine strains of YF, and out of these 13,16% (5/38) were from Jacupiranga and 86,84% (33/38) were from Teodoro Sampaio. Neutralizing antibodies were detected in 34% of the HI positive samples (13SN/38HI) and out of these 15,38% (2/13) were from Jacupiranga and 84,62% (11/13) were from Teodoro Sampaio. None of the samples were positive by MACELISA and RT-PCR indicating no evidences of recent virus circulation in the population analyzed in this study. However, the YF neutralizing antibodies detection in samples from Jacupiranga indicates the necessity of further researches in order to detect the YFV in its wild reservoirs and vectors. On the other hand, considering that 42,30% of the Teodoro Sampaio samples were positive by HI and out of these only 33,33% had neutralizing antibodies to the YFV, our results also pointed out to the importance of pos-vaccination protective immunity surveys, in order to evaluate the efficiency of the vaccines in endemic areas.