No description available.

This diploma thesis deals with detection of atrial fibrillation from HRV, classification of Poincare map and in the end the divide into two groups, one with detected atrial fibrillation and one not. The result is the decision on which variables are statistically significant for the identification of atrial fibrillations and which are not, and classification of the ECG signals.

This diploma thesis deals with detection of atrial fibrillation from HRV, classification of Poincare map and in the end the divide into two groups, one with detected atrial fibrillation and one not. The result is the decision on which variables are statistically significant for the identification of atrial fibrillations and which are not, and classification of the ECG signals with Bayes and Lavenberg-Marquardt neural networks. Bayes neural network with 23 neurons in hidden layer is best with F1 measure = 83,6 %, Sensitivity = 88,1 % and Specificity 94,5 %.

No description available.

In the Manufacturing Industry there is a subset of technologies referred to as Rapid Technologies which are those technologies that create the ability to compress the time to market for new products under development . Of this subset, Additive Fabrication (AF), or more commonly known as Rapid Prototyping (RP), acquires much attention due to its unique and futuristic approach to the production of physical parts directly from 3D CAD data, CT or MRI scans, or data from laser scanning systems by utilizing various techniques to consecutively generate cross-sectional layers of a given thickness upon the previous layer to form 3D objects. While Rapid Prototyping is the most common name for the production technology it is also referred to as Additive Manufacturing, Layer Based Manufacturing, Direct Digital Manufacturing, Free-Form Fabrication, and 3-Dimensional Printing. With over 35 manufacturers of Additive Fabrication equipment in 2006 , the selection of an AF process and material for a specific application can become a significant task, especially for those with little or no technical experience with the technology and to add to this challenge, many of the various processes have multiple material options to select from . This research was carried out in order to design and construct a system that would allow a person, regardless of their level of technical knowledge, to quickly and easily filter through the large number of Additive Fabrication processes and their associated materials in order to find the most appropriate processes and material options to create physical reproductions of any part. The selection methodology used in this paper is a collection of assumptions and rules taken from the author's viewpoint of how, in real world terms, the selection process generally takes place between a consumer and a service provider. The methodology uses those assumptions in conjunction with a set of expert based rules to direct the user to a best set of qualifying processes and materials suited for their application based on as many or as few input fields the user may be able to complete.

[ES] Los satélites de telecomunicación operan en entornos multiportadora, bajo una demanda continua de mayores capacidades de transmisión. Esto ha originado un aumento en los niveles de potencia de RF, frecuencias de trabajo, y número de canales transmitidos, estimulando la aparición de efectos no lineales de alta potencia, como Multipactor, Corona, y la Intermodulación Pasiva (PIM). Entre los efectos anteriores, el PIM es el menos comprendido, debido a su carácter no lineal y su estrecha relación con la fabricación, lo que dificulta el desarrollo de modelos fiables. Los términos de PIM generados en el enlace descendente pueden interferir a la débil señal en el canal de recepción, amenazando la capacidad de recepción del enlace ascendente. Los modelos tradicionales de PIM suelen basarse en una excitación formada por dos portadoras. Aunque se trata de un caso simple y bastante representativo, presenta diferencias importantes con el escenario real multiportadora. El trabajo de este Ph.D. intenta reducir estas diferencias. Para lograrlo, se realizan dos nuevas contribuciones de relevancia para las condiciones reales de operación de los satélites. En concreto, se ha investigado de forma teórica el rol de las fases de las portadoras en el PIM, y se ha propuesto un nuevo modelo que tiene en cuenta el efecto de las portadoras no contribuyentes en un cierto término de PIM, aplicando un nuevo principio de conservación de energía. Los resultados obtenidos con ambos modelos teóricos se ajustan a los datos experimentales. Debido a la compleja naturaleza del PIM, la validación de los componentes de RF de satélites se realiza mediante tests. Por lo tanto, la disponibilidad de bancos de medida de PIM es un tema de interés para la industria espacial. Sin embargo, el diseño de bancos de altas prestaciones es un desafío, ya que su nivel de PIM debe ser inferior al requerido para validar los dispositivos. Para hardware de satélites, la diferencia entre el nivel de las portadoras a transmitir y la señal de RF a detectar es como 185 dBc. En este Ph.D. se proponen unas nuevas arquitecturas integradas de bancos de PIM en guía de ondas, que cubren tanto el PIM conducido como el radiado. Dichas arquitecturas permiten una reducción importante del nivel de PIM residual del sistema de medida, siendo flexibles, capaces de manejar elevados niveles de potencia, y libres de resonancias e interacciones indeseadas. Los elementos claves de estos bancos son unos multiplexores de bajo PIM, que pueden incorporar dos familias de filtros que admiten un elevado número de ceros de transmisión, y por tanto, son capaces de proporcionar un elevado rechazo en la banda de recepción del PIM. Los bancos de medida de PIM conducido por onda reflejada, sin embargo, están expuestos al PIM generado por la carga empleada para absorber las portadoras de alta potencia. Para resolver esta situación, se han propuesto unas nuevas cargas de bajo PIM, que reducen el PIM residual de estos sistemas de medida. Así mismo, se ha elaborado un nuevo tipo de transición para mitigar el PIM respecto a flanges estándar. Para escenarios radiados se ha desarrollado una formulación capaz de relacionar densidades de potencia en el dispositivo bajo test con los niveles detectados en el banco de medida, y que por tanto permiten trasladar especificaciones de PIM del satélite al sistema de medida. Por último, se han mostrado varias campañas de medida de PIM realizadas con bancos implementados acorde a las nuevas arquitecturas propuestas. Las medidas cubren varias bandas de frecuencia y diferentes escenarios (tanto PIM conducido como radiado). Se ha determinado el excepcional nivel de fondo de ruido de PIM logrado en cada banco. Además, se han mostrado resultados obtenidos para medidas de PIM radiado en capas aislantes multicapa y mallas reflectoras, obteniendo interesantes conclusiones en cuanto a geometrías y impacto que tienen en el PIM elementos como los bordes serrados y los remaches. / [CA] Els satèl·lits de telecomunicació operen en entorns multiportadora, sota una demanda contínua de majors capacitats de transmissió. Això ha originat un augment en els nivells de potència de *RF, freqüències de treball, i nombre de canals transmesos, estimulant l'aparició d'efectes no lineals d'alta potència, com *Multipactor, Corona, i la Intermodulació Passiva (*PIM). Entre els efectes anteriors, el *PIM és el menys comprés, a causa del seu caràcter no lineal i la seua estreta relació amb la fabricació, la qual cosa dificulta el desenvolupament de models fiables. Els termes de *PIM generats en l'enllaç descendent poden interferir al feble senyal en el canal de recepció, amenaçant la capacitat de recepció de l'enllaç ascendent. Els models tradicionals de *PIM solen basar-se en una excitació formada per dues portadores. Encara que es tracta d'un cas simple i bastant representatiu, presenta diferències importants amb l'escenari real multiportadora. El treball d'aquest *Ph.D. intenta reduir aquestes diferències. Per a aconseguir-ho, es realitzen dues noves contribucions de rellevància per a les condicions reals d'operació dels satèl·lits . En concret, s'ha investigat de manera teòrica el rol de les fases de les portadores en el *PIM, i s'ha proposat un nou model que té en compte l'efecte de les portadores no contribuents en un cert terme de *PIM, aplicant un nou principi de conservació d'energia. Els resultats obtinguts amb tots dos models teòrics s'ajusten a les dades experimentals. A causa de la complexa naturalesa del *PIM, la validació dels components de *RF de satèl·lits es realitza mitjançant tests. Per tant, la disponibilitat de bancs de mesura de *PIM és un tema d'interés per a la indústria espacial. No obstant això, el disseny de bancs d'altes prestacions és un desafiament, ja que el seu nivell de *PIM ha de ser inferior al requerit per a validar els dispositius. Per a maquinari de satèl·lits , la diferència entre el nivell de les portadores a transmetre i el senyal de *RF a detectar és com 185 *dBc. En aquest *Ph.D. es proposen unes noves arquitectures integrades de bancs de *PIM en guia d'ones, que cobreixen tant el *PIM conduït com el radiat. Aquestes arquitectures permeten una reducció important del nivell de *PIM residual del sistema de mesura, sent flexibles, capaces de manejar elevats nivells de potència, i lliures de ressonàncies i interaccions indesitjades. Els elements claus d'aquests bancs són uns multiplexors de baix *PIM, que poden incorporar dues famílies de filtres que admeten un elevat nombre de zeros de transmissió, i per tant, són capaces de proporcionar un elevat rebuig en la banda de recepció del *PIM. Els bancs de mesura de *PIM conduït per ona reflectida, no obstant això, estan exposats al *PIM generat per la càrrega emprada per a absorbir les portadores d'alta potència. Per a resoldre aquesta situació, s'han proposat unes noves càrregues de baix *PIM, que redueixen el *PIM residual d'aquests sistemes de mesura. Així mateix, s'ha elaborat un nou tipus de transició per a mitigar el *PIM respecte a *flanges estàndard. Per a escenaris radiats s'ha desenvolupat una formulació capaç de relacionar densitats de potència en el dispositiu sota test amb els nivells detectats en el banc de mesura, i que per tant permeten traslladar especificacions de *PIM del satèl·lit al sistema de mesura. Finalment, s'han mostrat diverses campanyes de mesura de *PIM realitzades amb bancs implementats concorde a les noves arquitectures proposades. Les mesures cobreixen diverses bandes de freqüència i diferents escenaris (tant *PIM conduït com radiat). S'ha determinat l'excepcional nivell de fons de soroll de *PIM reeixit en cada banc. A més, s'han mostrat resultats obtinguts per a mesures de *PIM radiat en capes aïllants multicapa i malles reflectores, obtenint interessants conclusions quant a geometries i impacte que tenen en el *PIM elements com les vores serrades i els reblons. / [EN] Modern satellite payloads operate in multicarrier scenarios, under a continuous demand for higher capacity links. This leads to an increase in the RF power levels, frequency of operation, and the number of transmitted channels, thus stimulating non-linear high-power effects, such as Multipactor, Corona, thermal issues and Passive Inter-Modulation (PIM). Among the above-mentioned phenomena, PIM is the less studied, or, at least, understood. This is due to its extreme non-linear nature and its close relation to workmanship, which make very difficult the development of models able to faithfully predict and explain PIM degradation. PIM terms, once ignited in the downlink, may interfere the weak signal to be detected in the uplink channel, thus threatening the payload throughput. Traditional PIM models are based on a two-carriers excitation. This is a simple and quite representative case, but has significant differences with the real multi- carrier scenario. This Ph.D. thesis work tries to diminish this gap by two novel contributions of relevance for real operation conditions. Firstly, the role of the carrier phases (neglected for two-carriers excitation) has been theoretically investigated. Secondly, a new model to account for the effect of non-contributing carriers for a given PIM term has been developed, which is based on a novel energy conservation assumption. The resulting models fit to experimental data. Due to the complexity of PIM modeling, PIM validation of RF components is conducted only by testing. The availability of low PIM test set-ups is therefore of great interest for the space industry. However, the design of low PIM test benches is challenging, as their intrinsic residual PIM has to be below the one requested to validate the test devices. For satellite hardware, the dynamic range between the RF power levels of the transmission carriers and the signal to be detected may be 185 dBc. During this Ph.D. thesis work, novel integrated test bed architectures in waveguide technology, both for conducted and radiated PIM scenarios, have been developed. These architectures consent a mitigation of the residual PIM of the test facility, being at the same time flexible, free from unwanted interactions and spurious resonances, and able to withstand considerable RF power levels for the transmission carriers. The key elements of these set-ups are the low PIM multiplexers, which may integrate two new families of waveguide filters able to provide a high number of transmission zeros, and therefore a high rejection, in the PIM reception channel. The test benches conceived for measuring conducted backward PIM, however, are normally unprotected from the PIM generated by the termination absorbing the high-power transmission carriers. To alleviate this situation, a new type of low PIM terminations in waveguide technology has been proposed and verified with PIM tests, showing a clear benefit in mitigating the residual PIM of the test facilities. Moreover, novel transitions able to improve the PIM performance of standard flanges have also been conceived. Finally, and with regard to radiated scenarios, a novel formulation able to convert payload PIM specifications to a practical PIM test is proposed. This formulation consents to link the power flux densities at the device under test (DUT) with the RF power levels measured by the test bench. Last, a large class of PIM measurements carried out with the novel test bed architectures have been reported. These measurements cover several frequency bands (C-, Ku-, K- and Ka) and different PIM scenarios, both conducted and radiated. The exceptional residual PIM noise floor of each test bed will be pointed out. In addition, PIM tests on an anechoic chamber facility, multi-layer insulation blankets (MLIs) and reflector mesh samples are presented, with interesting considerations about the geometry of the structure and the impact on the PIM performance of typical elements as sawing areas and rivets. / Smacchia, D. (2022). Advanced Techniques for the Characterization and Experimental Validation of Passive Inter-Modulation Effect (PIM) in Space Communications Systems [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/182402

Atrial fibrillation is a common heart arrhythmia which is characterized by a missing or irregular contraction of the atria. The disease is a risk factor for other more serious diseases and the total medical costs in society are extensive. Therefore it would be beneficial to improve and optimize the prevention and detection of the disease.   Pulse palpation and heart auscultation can facilitate the detection of atrial fibrillation clinically, but the diagnosis is generally confirmed by an ECG examination. Today there are several algorithms that detect atrial fibrillation by analysing an ECG. A common method is to study the heart rate variability (HRV) and by different types of statistical calculations find episodes of atrial fibrillation which deviates from normal sinus rhythm.   Two algorithms for detection of atrial fibrillation have been evaluated in Matlab. One is based on the coefficient of variation and the other uses a logistic regression model. Training and testing of the algorithms were done with data from the Physionet MIT database. Several steps of signal processing were used to remove different types of noise and artefacts before the data could be used.   When testing the algorithms, the CV algorithm performed with a sensitivity of 91,38%, a specificity of 93,93% and accuracy of 92,92%, and the results of the logistic regression algorithm was a sensitivity of 97,23%, specificity of 93,79% and accuracy of 95,39%. The logistic regression algorithm performed better and was chosen for implementation in Java, where it achieved a sensitivity of 97,31%, specificity of 93,47% and accuracy of 95,25%. / Förmaksflimmer är en vanlig hjärtrytmrubbning som kännetecknas av en avsaknad eller oregelbunden kontraktion av förmaken. Sjukdomen är en riskfaktor för andra allvarligare sjukdomar och de totala kostnaderna för samhället är betydande. Det skulle därför vara fördelaktigt att effektivisera och förbättra prevention samt diagnostisering av förmaksflimmer.   Kliniskt diagnostiseras förmaksflimmer med hjälp av till exempel pulspalpation och auskultation av hjärtat, men diagnosen brukar fastställas med en EKG-undersökning. Det finns idag flertalet algoritmer för att detektera arytmin genom att analysera ett EKG. En av de vanligaste metoderna är att undersöka variabiliteten av hjärtrytmen (HRV) och utföra olika sorters statistiska beräkningar som kan upptäcka episoder av förmaksflimmer som avviker från en normal sinusrytm.   I detta projekt har två metoder för att detektera förmaksflimmer utvärderats i Matlab, en baseras på beräkningar av variationskoefficienten och den andra använder sig av logistisk regression. EKG som kommer från databasen Physionet MIT används för att träna och testa modeller av algoritmerna. Innan EKG-signalen kan användas måste den behandlas för att ta bort olika typer av brus och artefakter.   Vid test av algoritmen med variationskoefficienten blev resultatet en sensitivitet på 91,38%, en specificitet på 93,93% och en noggrannhet på 92,92%. För logistisk regression blev sensitiviteten 97,23%, specificiteten 93,79% och noggrannheten 95,39%. Algoritmen med logistisk regression presterade bättre och valdes därför för att implementeras i Java, där uppnåddes en sensitivitet på 91,31%, en specificitet på 93,47% och en noggrannhet på 95,25%.

Os eicosanoides, produtos do metabolismo do ácido araquidônico, apresentam papel importante na homeostasia e na patogênese de diversas doenças humanas. A biossíntese desses compostos pode ser estimulada por agentes farmacológicos como ionóforos e inibidores da Ca2+-ATPase, e também por agonistas naturais como o formil-metionil-leucil-fenialanina (fMLP). Considerando os interesses em avaliar e comparar o perfil de mediadores lipídicos, como os leucotrienos (LTs), as prostaglandinas (PGs), os ácidos epoxieicosatrienoicos (EETs), os ácidos dihidroxitetraenoicos (DiHETEs) e os ácidos hidroxieicosatetraenoicos (HETEs), na saúde e na doença, o objetivo deste trabalho foi padronizar um método analítico para determinar do perfil de eicosanoides em plasma humano após estimulação do sangue total, e assim observar diferenças entre indivíduos saudáveis e doentes. Dessa forma, um método por cromatografia líquida de alta eficiência acoplada à espectrometria de massas sequencial (HPLC-MS/MS) foi validado para quantificação de 22 eicosanoides em plasma de indivíduos saudáveis. A análise por HPLCMS/ MS foi realizada em modo negativo pelo modo de varredura por monitoramento de reações múltiplas (MRM). A linearidade do método apresentou coeficiente de correlação (r) maior que 0,98 para todos os eicosanoides analisados. A precisão e exatidão intra e inter-ensaios tiveram desvio padrão e erro relativo menores que 15%, exceto para o limite inferior de quantificação cujos valores foram menores que 20%. Para estimulação das células do sangue total, quatro estímulos (fMLP, ionomicina, A23187 e tapsigargina) foram utilizados. A análise estatística mostrou que o A23187 e a tapsigargina foram os estímulos mais potentes na indução da produção de eicosanoides. Em seguida, comparamos o perfil de eicosanoides em amostras de plasma de indivíduos saudáveis com pacientes com anemia falciforme (AF), em tratamento com hidroxiureia (HU) ou transfusão sanguínea crônica. Os resultados demonstraram que o método é preciso para determinação de diferenças entre os pacientes e indivíduos saudáveis quanto à produção dos mediadores lipídicos 5-HETE, 12-HETE, LTB4, LTE4, TXB2 e PGE2. Portanto, nosso método analítico é sensível, específico e reprodutível para identificar e quantificar diferenças no perfil de eicosanoides em amostras de sangue estimuladas in vitro, e poderá contribuir para o estabelecimento do perfil de mediadores lipídicos em diferentes doenças inflamatórias e infecciosas. / Eicosanoids, products from arachidonic acid metabolism, play an important role in the homeostasis and in the pathogenesis of various human diseases. Pharmacological agents such as Ca2+ ionophores and Ca2+-ATPase inhibitors, as well as natural agonists such as fMet-leu-Phe (fMLP) can stimulate eicosanoid biosynthesis. Considering the interests in evaluate and compare the profile of lipid mediators, as leukotriens (LTs), prostaglandins (PGs), epoxyeicosatrienoic acids (EETs), dihydroxytetraenoic acids (DiHETEs) and hydroxyeicosatetraenoic acids (HETEs), in healthy and disease, the aim of this work was to standardize a method to determine the eicosanoid profile of human plasma samples after whole blood stimulation, and to assess differences between healthy and sick individuals. For this purpose, a liquid chromatographytandem mass spectrometry (LC-MS/MS) method was validated for the quantification of 22 eicosanoids using human plasma from healthy volunteers. In addition, we optimized a method for the stimulation of eicosanoids in human whole blood. LC-MS/MS analyses were performed by negative electrospray ionization and multiple reaction monitoring. An assumption of linearity resulted in a regression coefficient > 0.98 for all eicosanoids tested. The mean intra-assay and inter-assay accuracy and precision values had relative standard deviations and relative errors of < 15%, except for the lower limit of quantification, where these values were < 20%. For whole blood stimulation, four stimuli (fMLP, ionomycin, A23187, and thapsigargin) were used. Results of the statistical analysis showed that A23187 and thapsigargin were potent stimuli to induce the production of eicosanoids. We next compared the eicosanoid profiles of healthy volunteers to those of patients with sickle cell anemia (SCA) under treatment with hydroxyurea (HU) or after chronic red blood cell (RBC) transfusion. The results indicate that the method was sufficient to find a difference between lipid mediators released in whole blood of SCA patients compared to healthy subjects for 5-HETE, 12-HETE, LTB4, LTE4, TXB2, and PGE2. In conclusion, our analytical method is sensitive, specific and reproducible for indentify and quantify changes in eicosanoid profiles in whole blood stimulated in vitro, which can contribute to establishing the eicosanoid profiles associated with different inflammatory and infectious diseases.

Bengt Lidforss (1868–1913) was professor of botany between 1910 and 1913. But after the turn of the century he also emerged as a charismatic leader within the Swedish working-class movement. He became one of its foremost publicists. In the social democratic newspaper Arbetet in Malmö he wrote about natural sciences but also about political, philosophical and literary issues. As a literary critic Lidforss was the keenest protector of the Scanian literary school, and his struggle for Ola Hansson and Vilhelm Ekelund has made its mark in Swedish literary history, as have his contributions in favour of Gustaf Fröding and August Strindberg, culminating in the polemic articles during the Strindberg Feud (1910–11). Skönhetsdyrkare och socialdemokrat. Studier i Bengt Lidforss litteraturkritiska gärning (Worshipper of Beauty And Social Democrat. Studies in Bengt Lidforss’ Achievement As A Literary Critic) emphasises the paradoxic combination of Lidforss’ fundamentally socialist views and a strong belief in art. To him art was not isolated from society but quite the contrary; a significant factor in the changing of society. The new socialistic human being should be ennobled by arts instead of emasculated by religion. With the help of Pierre Bourdieu’s concept of “field”, it is shown how Lidforss, by attacking leading middle-class newspapers and publicists, created for himself and Arbetet a constantly stronger position within the field of journalism. Within the field of literary criticism he attacked the middle-class critics, and thus participated in associating Strindberg and Fröding as well as the young Scanian writers with the working-class movement. Lidforss’ literary taste was seen as an alternative to middle-class taste and the worshipping of beauty thereby became fashionable among socialists. The fact that one finds sympathies not only for symbolism but also for decadent descriptions with Lidforss the socialist, has to do with the fact that the descriptions of the discomfort of the heroes revealed the disadvantages of the capitalist society. Nevertheless Lidforss’ issued warnings against programmatic pessimism, since he was of the opinion that literature should strengthen people in their struggle. When it came to the plight of the human being under capitalism he was a pessimist, but he believed the stronger in a future socialist society. The terms for the artists would be more tolerable in such a society, he prophesied. He admitted that revolutionary poetry could be useful but was of the opinion that the quality of art would lessen if it consciously served politics. The revolutionary poetry he praised in his reviews was poetry he found genuine and coming from the heart. He did not favour pronounced tendencies, but he liked to use poetry in a political context. / Bengt Lidforss (1868–1913) var botaniker, men också publicist och socialist. Skandalomsusad och färgstark har han porträtterats av ett stort antal skönlitterära författare, allt ifrån August Strindberg till Inger Alfvén. Hans mångsidiga medarbetarskap i Arbetet hjälpte tidningen fram till en uppmärksammad position. I denna bok skildras hans kamp för en ledande position också inom det litterära fältet. Lidforss var en skönhetsdyrkare av stora mått men samtidigt socialdemokrat. Detta ledde till att han stred på många kulturella arenor – inte bara mot kritiker, författare, och Svenska Akademien, utan också mot inflytelserika män inom kyrka och politik. Skönhetsdyrkare och socialdemokrat ger oss oväntade svar på vad dessa bataljer handlade om och vi får möta Lidforss samtida giganter som Fredrik Böök, Vilhelm Ekelund, Albert Engström, Verner von Heidenstam, Oscar Levertin, August Strindberg med flera.

Os eicosanoides, produtos do metabolismo do ácido araquidônico, apresentam papel importante na homeostasia e na patogênese de diversas doenças humanas. A biossíntese desses compostos pode ser estimulada por agentes farmacológicos como ionóforos e inibidores da Ca2+-ATPase, e também por agonistas naturais como o formil-metionil-leucil-fenialanina (fMLP). Considerando os interesses em avaliar e comparar o perfil de mediadores lipídicos, como os leucotrienos (LTs), as prostaglandinas (PGs), os ácidos epoxieicosatrienoicos (EETs), os ácidos dihidroxitetraenoicos (DiHETEs) e os ácidos hidroxieicosatetraenoicos (HETEs), na saúde e na doença, o objetivo deste trabalho foi padronizar um método analítico para determinar do perfil de eicosanoides em plasma humano após estimulação do sangue total, e assim observar diferenças entre indivíduos saudáveis e doentes. Dessa forma, um método por cromatografia líquida de alta eficiência acoplada à espectrometria de massas sequencial (HPLC-MS/MS) foi validado para quantificação de 22 eicosanoides em plasma de indivíduos saudáveis. A análise por HPLCMS/ MS foi realizada em modo negativo pelo modo de varredura por monitoramento de reações múltiplas (MRM). A linearidade do método apresentou coeficiente de correlação (r) maior que 0,98 para todos os eicosanoides analisados. A precisão e exatidão intra e inter-ensaios tiveram desvio padrão e erro relativo menores que 15%, exceto para o limite inferior de quantificação cujos valores foram menores que 20%. Para estimulação das células do sangue total, quatro estímulos (fMLP, ionomicina, A23187 e tapsigargina) foram utilizados. A análise estatística mostrou que o A23187 e a tapsigargina foram os estímulos mais potentes na indução da produção de eicosanoides. Em seguida, comparamos o perfil de eicosanoides em amostras de plasma de indivíduos saudáveis com pacientes com anemia falciforme (AF), em tratamento com hidroxiureia (HU) ou transfusão sanguínea crônica. Os resultados demonstraram que o método é preciso para determinação de diferenças entre os pacientes e indivíduos saudáveis quanto à produção dos mediadores lipídicos 5-HETE, 12-HETE, LTB4, LTE4, TXB2 e PGE2. Portanto, nosso método analítico é sensível, específico e reprodutível para identificar e quantificar diferenças no perfil de eicosanoides em amostras de sangue estimuladas in vitro, e poderá contribuir para o estabelecimento do perfil de mediadores lipídicos em diferentes doenças inflamatórias e infecciosas. / Eicosanoids, products from arachidonic acid metabolism, play an important role in the homeostasis and in the pathogenesis of various human diseases. Pharmacological agents such as Ca2+ ionophores and Ca2+-ATPase inhibitors, as well as natural agonists such as fMet-leu-Phe (fMLP) can stimulate eicosanoid biosynthesis. Considering the interests in evaluate and compare the profile of lipid mediators, as leukotriens (LTs), prostaglandins (PGs), epoxyeicosatrienoic acids (EETs), dihydroxytetraenoic acids (DiHETEs) and hydroxyeicosatetraenoic acids (HETEs), in healthy and disease, the aim of this work was to standardize a method to determine the eicosanoid profile of human plasma samples after whole blood stimulation, and to assess differences between healthy and sick individuals. For this purpose, a liquid chromatographytandem mass spectrometry (LC-MS/MS) method was validated for the quantification of 22 eicosanoids using human plasma from healthy volunteers. In addition, we optimized a method for the stimulation of eicosanoids in human whole blood. LC-MS/MS analyses were performed by negative electrospray ionization and multiple reaction monitoring. An assumption of linearity resulted in a regression coefficient > 0.98 for all eicosanoids tested. The mean intra-assay and inter-assay accuracy and precision values had relative standard deviations and relative errors of < 15%, except for the lower limit of quantification, where these values were < 20%. For whole blood stimulation, four stimuli (fMLP, ionomycin, A23187, and thapsigargin) were used. Results of the statistical analysis showed that A23187 and thapsigargin were potent stimuli to induce the production of eicosanoids. We next compared the eicosanoid profiles of healthy volunteers to those of patients with sickle cell anemia (SCA) under treatment with hydroxyurea (HU) or after chronic red blood cell (RBC) transfusion. The results indicate that the method was sufficient to find a difference between lipid mediators released in whole blood of SCA patients compared to healthy subjects for 5-HETE, 12-HETE, LTB4, LTE4, TXB2, and PGE2. In conclusion, our analytical method is sensitive, specific and reproducible for indentify and quantify changes in eicosanoid profiles in whole blood stimulated in vitro, which can contribute to establishing the eicosanoid profiles associated with different inflammatory and infectious diseases.