Submitted by Automação e Estatística (sst@bczm.ufrn.br) on 2018-05-03T23:39:03Z No. of bitstreams: 1 NatanReygesCastroDaPurificacao_DISSERT.pdf: 1600748 bytes, checksum: 9f0aeca5d6e5d013f0238d4f331f5ccd (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2018-05-14T23:42:52Z (GMT) No. of bitstreams: 1 NatanReygesCastroDaPurificacao_DISSERT.pdf: 1600748 bytes, checksum: 9f0aeca5d6e5d013f0238d4f331f5ccd (MD5) / Made available in DSpace on 2018-05-14T23:42:52Z (GMT). No. of bitstreams: 1 NatanReygesCastroDaPurificacao_DISSERT.pdf: 1600748 bytes, checksum: 9f0aeca5d6e5d013f0238d4f331f5ccd (MD5) Previous issue date: 2018-01-29 / A cardiomiopatia diabética (CM) é uma das maiores causas de morbidade e mortalidade em pacientes diabéticos e, ela é caracterizada por hipertrofia e fibrose cardíaca. Este trabalho teve como objetivo analisar os efeitos do tratamento conjunto de antioxidantes no miocárdio de ratos com diabetes mellitus (DM). Foram utilizados 20 ratos machos com 90 dias de subdivididos em quatro grupos (n=5): N (normoglicêmico); D (diabético); NT (normoglicêmico tratado com antioxidantes) e; DT (diabético tratados com antioxidantes). O DM foi induzido por estreptozootocina (35mg/ kg de peso corporal). O tratamento antioxidativo foi realizado via gavagem com: quercetina (100mg/kg de peso corporal), L- glutamina 1% e α-tocoferol 1% e o período experimental foi de 60 dias. Foram analisados o peso corporal dos animais, consumo de água e de ração. As análises da glicemia final, MDA, GSH, IL-1β, NTF-α, IL-10 e MPO, foram realizadas no tecido sanguíneo enquanto que as análises quantitativas de fibras colágenas e da expressão de SOD-1, GPX-1, IL-1β, TGF-β e FGF-2 foram realizadas em secções do miocárdio com 3μm de espessura. O teste de Fisher foi utilizado com nível de significância quando p<0,05%. Os resultados demonstraram redução do peso corporal em D e DT em relação aos normoglicêmicos e, foi observado aumento do coeficiente peso do coração/peso corporal final (PC/PF), um indicativo de hipertrofia cardíaca, nos grupos D, DT e NT em relação a N (p<0,05). O consumo de água e ração foi maior em D, NT e DT em comparação a N, sendo que o grupo DT apresentou maiores médias que os demais (p<0,05). A hiperglicemia foi confirmada nos animais de D e DT em relação ao não diabéticos, sendo maior em DT que em D (p<0,05). Em relação ao estresse oxidativo sistêmico houve aumento dos níveis plasmáticos de MDA em NT e DT em comparação a N e D (p<0,05) e, aumento de GSH em DT em relação a D (p<0,001) e em NT em comparação a N (p<0,001). A nível tecidual ocorreu aumento da expressão de SOD-1 no miocárdio dos animais do grupo D em relação a N (p<0,001) enquanto que GPX foi maior em D e em NT em relação a N (p<0,001) e menor em DT em relação a D (p<0,001). Na análise da inflamação sistêmica houve aumento do nível de MPO em NT em relação a N (p<0,001), os níveis de IL-1β e de TNF-α foram semelhantes entre os grupos e, a IL-10 foi maior em DT que em D (p<0,01) e menor em NT em comparação a N (p<0,01). A nível tecidual ocorreu aumento da expressão da IL-1β em D quando comparado com N (p<0,001). Em relação ao desenvolvimento tecidual houve aumento da expressão do TGF-β no grupo D em comparação a N (p<0,001), aumento do FGF-2 em DT em relação aos demais grupos (p<0,001) e, aumento da deposição de fibras colágenas em D e NT se comparado a N (p<0,001). Os achados indicam que o tratamento conjunto de antioxidantes proposto nesta pesquisa não foi capaz de reverter ou diminuir as alterações decorrentes do DM em relação ao peso corporal, razão peso do coração/peso corporal, níveis plasmáticos de glicemia, quantidade de água e ração ingeridos. O tratamento não foi benéfico nos animais normoglicêmicos uma vez que também foi observado hipertrofia cardíaca e aumento do consumo de água e ração. A nível sistêmico o DM reduziu os níveis de IL-10 enquanto que o tratamento promoveu maior estresse oxidativo (níveis de MDA) e de inflamação (níveis de MPO) nos animais do grupo DT, ao mesmo tempo que estimulou o aumento do GSH e da IL-10 que possuem ação antioxidante e anti-inflamatória, respectivamente. Porém, em NT os achados de estresse foram semelhantes a DT, mas em relação aos marcadores de inflamação houve aumento do MPO e redução da IL-10, indicando que o tratamento foi nocivo nos animais normais. No miocárdio o DM promoveu aumento da expressão de SOD-1, GPX, IL-1β, TGF-β e na deposição de colágeno. O tratamento antioxidante em DT não promoveu redução do SOD-1, da IL-1β e do TGF- β, porém reduziu o estresse oxidativo (expressão de GPX-1) pela maior ativação da via da glutationa, que levou a maior expressão de FGF-2 e redução da deposição de colágeno. No grupo NT, contudo, houve aumento do estresse oxidativo (GPX-1) que levou a maior deposição de colágeno em relação aos demais grupos, demonstrando novamente que o uso de antioxidantes em condição de normalidade não é indicado. Desta forma, o tratamento conjunto com quercetina, L-glutamina e α-tocoferol promoveu redução do estresse oxidativo e da fibrose no miocárdio de ratos com diabetes mellitus induzida. / Diabetic cardiomyopathy (CM) is one of the major causes of morbidity and mortality in diabetic patients and is characterized by hypertrophy and cardiac fibrosis. This work aimed to analyze the effects of the joint treatment of antioxidants in the myocardium of rats with diabetes mellitus (DM). Twenty-nine male rats with 90 days of subdivision were used in four groups (n = 5): N (normoglycemic); D (diabetic); NT (normoglycemic treated with antioxidants) and; DT (diabetic treated with antioxidants). DM was induced by streptozotocin (35mg / kg body weight). The antioxidative treatment was performed via gavage with quercetin (100mg / kg body weight), 1% L-glutamine and 1% α-tocopherol and the experimental period was 60 days. The animals' body weight, water consumption and feed intake were analyzed. The analysis of the final glycemia, MDA, GSH, IL-1β, NTF-α, IL-10 and MPO, were performed in the blood tissue while the quantitative analyzes of collagen fibers and expression of SOD-1, GPX-1, IL -1β, TGF-β and FGF-2 were performed in 3-m thick sections of the myocardium. Fisher's test was used with level of significance when p <0.05%. The results showed a reduction in body weight in D and DT compared to normoglycemics, and an increase in heart weight / final body weight (PC / PF), an indication of cardiac hypertrophy, was observed in groups D, DT and NT in relation to N (p <0.05). The water and feed intake was higher in D, NT and DT compared to N, and DT group presented higher mean values than the others (p <0.05). Hyperglycemia was confirmed in the D and DT animals in relation to nondiabetics, being higher in DT than in D (p <0.05). In relation to systemic oxidative stress there was an increase in the plasma levels of MDA in NT and DT compared to N and D (p <0.05) and, increase of GSH in DT in relation to D (p <0.001) and NT in compared to N (p <0.001). At the tissue level, there was an increase in the expression of SOD-1 in the myocardium of the animals of group D in relation to N (p <0.001), whereas GPX was higher in D and in NT compared to N (p <0.001) and lower in DT with respect to D (p <0.001). In the analysis of systemic inflammation there was an increase in the level of MPO in NT compared to N (p <0.001), levels of IL-1β and TNF-α were similar between groups and IL-10 was higher in DT than in D (p <0.01) and lower in NT compared to N (p <0.01). At the tissue level there was an increase in the expression of IL-1β in D when compared to N (p <0.001). In relation to tissue development there was an increase in the expression of TGF-β in group D compared to N (p <0.001), increase in FGF-2 in DT in relation to the other groups (p <0.001) and increase in fiber deposition collagen in D and NT compared to N (p <0.001). The findings indicate that the antioxidant combination treatment proposed in this study was not able to reverse or decrease DM changes in relation to body weight, heart weight / body weight ratio, plasma glucose levels, amount of water and ingested ration. Treatment was not beneficial in normoglycemic animals since cardiac hypertrophy and increased water and feed consumption were also observed. At the systemic level DM reduced IL-10 levels while the treatment promoted higher oxidative stress (MDA levels) and inflammation (MPO levels) in the DT group, while stimulating the increase in GSH and IL-10 that have antioxidant and anti-inflammatory action, respectively. However, in NT the stress findings were similar to DT, but in relation to inflammation markers there was an increase in MPO and a reduction in IL-10, indicating that the treatment was harmful in normal animals. In the myocardium the DM promoted increased expression of SOD-1, GPX, IL-1β, TGF-β and in collagen deposition. The antioxidant treatment in DT did not promote reduction of SOD-1, IL-1β and TGF-β, but it reduced oxidative stress (expression of GPX-1) by the greater activation of the glutathione pathway, which led to greater expression of FGF -2 and reduction of collagen deposition. In the NT group, however, there was an increase in oxidative stress (GPX-1), which led to higher collagen deposition in relation to the other groups, again demonstrating that the use of antioxidants in a normal condition is not indicated. Thus, treatment with quercetin, L-glutamine and α-tocopherol promoted reduction of oxidative stress and fibrosis in the myocardium of rats with induced diabetes mellitus.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-29T00:30:04Z No. of bitstreams: 1 MariaPatriciaOliveiraDaSilvaReinaldo_DISSERT.pdf: 1968663 bytes, checksum: 9310ea8c13185d093751709ea668017c (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-29T19:38:59Z (GMT) No. of bitstreams: 1 MariaPatriciaOliveiraDaSilvaReinaldo_DISSERT.pdf: 1968663 bytes, checksum: 9310ea8c13185d093751709ea668017c (MD5) / Made available in DSpace on 2016-03-29T19:38:59Z (GMT). No. of bitstreams: 1 MariaPatriciaOliveiraDaSilvaReinaldo_DISSERT.pdf: 1968663 bytes, checksum: 9310ea8c13185d093751709ea668017c (MD5) Previous issue date: 2015-03-19 / A mucosite intestinal ? a inflama??o e/ou ulcera??o da mucosa do trato gastrointestinal causada pelas terapias antic?ncer. Apresenta histologicamente, atrofia das vilosidades, danos nos enter?citos e infiltra??o de c?lulas inflamat?rias. O metotrexato ? um composto que inibe a dihidrofolato redutase, enzima importante na s?ntese de DNA. ? amplamente utilizado no tratamento de leucemia e outras malignidades. O objetivo deste estudo foi avaliar o efeito da Olmesartana (OLM), um antagonista do receptor da angiotensina II, em um modelo de mucosite intestinal (MMI) induzida por MTX em ratos Wistar. MMI foi induzido atrav?s de inje??o intraperitoneal (i.p.) de MTX (7 mg/kg) durante tr?s dias consecutivos. Os animais foram pr?-tratados com OLM oral a 0.5 mg/kg, 1 mg/kg e 5 mg/kg e com solu??o salina, 30 minutos antes da exposi??o ao MTX durante tr?s dias. Fragmentos de intestino delgado (duodeno, jejuno e ?leo) foram homogeneizados para ensaio de pesquisa das citocinas L-1?, IL-10 e TNF-?, atividade do Malonalde?do (MDA) e da Mieloperoxidase (MPO). Al?m disso, an?lises de imunohistoqu?mica da MMP-2, MMP-9, COX-2, RANK / RANKL e SOCS-1 al?m da an?lise da co-localiza??o da express?o de SOCS-1 pela microscopia confocal foram realizadas. O tratamento com MTX+OLM 5 mg/kg resultou numa redu??o da infiltra??o inflamat?ria da mucosa, ulcera??es, vasodilata??o e ?reas hemorr?gicas (p<0,05), bem como as concentra??es reduzidas de MPO (p<0,001) e as citocinas pr?- inflamat?rias IL-1? e TNF-? (p <0,01). Al?m disso, o tratamento combinado reduziu a express?o de MMP-2, MMP-9, COX-2, RANK e RANKL (p<0,05) e aumentou a express?o citoplasm?tica de SOCS-1 (p<0,05). Nossos achados confirmam o envolvimento de OLM na redu??o da resposta inflamat?ria atrav?s do aumento da sinaliza??o imunossupressora em MMI. Sugerimos tamb?m que o efeito ben?fico do tratamento com a Olmesartana ? especificamente exercida durante o dano atrav?s do bloqueio de citocinas inflamat?rias. / Intestinal Mucositis is inflammation and/or ulceration of mucosa of the gastrointestinal tract caused by anticancer therapies. Histologically, villous atrophy, damage to enterocytes and infiltration of inflammatory cells. Methotrexate (MTX) is a compound that depletes dihydrofolate pools and is widely used in the treatment of leukemia and other malignancies. The aim of this study was to evaluate the effect of Olmesartan (OLM), an angiotensin II receptor antagonist, on an Intestinal Mucositis Model (IMM) induced by MTX in Wistar rats. IMM was induced via intraperitoneal (i.p.) administration of MTX (7 mg/kg) for three consecutive days. The animals were pretreated with oral OLM at 0.5, 1 or 5 mg/kg or with vehicle 30 min prior to exposure to MTX, for three days. Small intestinal (duodenum, jejunum and ileum) homogenates were assayed for levels of the IL-1?, IL-10 and TNF-? cytokines, malondialdehyde and myeloperoxidase activity. Additionally, immunohistochemical analyses of MMP-2, MMP-9, COX-2, RANK/RANKL and SOCS-1 and confocal microscopy analysis of SOCS-1 expression were performed. Treatment with MTX+OLM (5 mg/kg) resulted in a reduction of mucosal inflammatory infiltration, ulcerations, vasodilatation and hemorrhagic areas (p<0.05) as well as reduced concentrations of MPO (p<0.001) and the pro-inflammatory cytokines IL-1? and TNF-? (p<0.01), and increase antiinflammatory cytosine IL-10 (p,0.05). Additionally, the combined treatment reduced expression of MMP-2, MMP-9, COX-2, RANK and RANKL (p<0.05) and increased cytoplasmic expression of SOCS-1 (p<0.05). Our findings confirm the involvement of OLM in reducing the inflammatory response through increased immunosuppressive signaling in an IMM. We also suggest that the beneficial effect of Olmesartan treatment is specifically exerted during the damage through blocking inflammatory cytosines.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-16T00:10:35Z No. of bitstreams: 1 JanineKarlaFrancaDaSilvaBraz_DISSERT.pdf: 1825350 bytes, checksum: f191d4fa09c99aba33d4591a0363c221 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-22T20:19:08Z (GMT) No. of bitstreams: 1 JanineKarlaFrancaDaSilvaBraz_DISSERT.pdf: 1825350 bytes, checksum: f191d4fa09c99aba33d4591a0363c221 (MD5) / Made available in DSpace on 2016-04-22T20:19:08Z (GMT). No. of bitstreams: 1 JanineKarlaFrancaDaSilvaBraz_DISSERT.pdf: 1825350 bytes, checksum: f191d4fa09c99aba33d4591a0363c221 (MD5) Previous issue date: 2015-06-29 / Objetivou-se avaliar as altera??es na espermatog?nese provocadas pela Distrofia Muscular de Duchenne (DMD) e o efeito do tratamento com ?cido asc?rbico na preven??o dessas inj?rias. Neste trabalho foram utilizados 24 camundongos, sendo 12 linhagem C57BL/10 (n?o-distr?ficos) e 12 C57BL/10Mdx (distr?ficos) divididos em seis grupos com 4 animais cada (C30 = Controle de 30 dias; D30 = Distr?fico com 30 dias; C60 = Controle com 60 dias; D60 = Distr?fico com 60 dias; CS = Controle com 60 dias suplementados com ?cido asc?rbico e; DS60 = Distr?fico com 60 dias suplementados com ?cido asc?rbico. Os animais dos grupos C30 e D30 foram eutanasiados aos trinta dias de idade, enquanto que os animais dos demais grupos aos 60 dias de idade. A suplementa??o com ?cido asc?rbico foi ministrada na ?gua na dosagem de 0,005g/dia durante 30 dias. Ap?s eutanasiados, os test?culos (direito e esquerdo) foram coletados, imediatamente, pesados e seccionados transversalmente, fixados em solu??o de Karnovysky, inclu?dos em resina histol?gica (an?lises morfol?gicas e morfom?tricas), submetidos a an?lise ultraestrutural e submentidos t?cnica de imunohistoqu?mica para caspase-3. Houve aumento significativo no percentual de t?nica pr?pria em D30 em rela??o a C30 e D60. As an?lises ultraestruturais indicaram ind?cios de apoptose mitocondrial das c?lulas de Sertoli que podem reduzir a efici?ncia esperm?tica em CS60 e DS60. Verificou-se maior densidade de volume das c?lulas apopt?ticas postivas para Caspase - 3 em D30 versus C30 e DS60 em rela??o a CS60. . Houve acentuada hipertrofia de c?lulas de Leydig entre D30 e D60. No entanto, com a suplementa??o observou-se revers?o dessa altera??o em DS60. Na ultraestrutura das c?lulas de Leydig observou-se a presen?a precoce de ves?culas lip?dicas no grupo distr?fico pr?-p?bere (D30). Dessa forma, a DMD afetou a organiza??o dos t?bulos semin?feros e intert?bulos, no entanto, a suplementa??o de ?cido asc?rbico., nas condi??es experimentais utilizadas para o tratamento da DMD foi suficiente apenas para reduzir a hipertofia das c?lulas de Leydig. / The objective was to evaluate spermatogenesis alterations caused by DMD and the effect of the treatment using ascorbic acid in preventing those injuries. Twenty four mice were used, 12 from the C57BL/10 lineage (non-dystrophic) and 12 from the C57BL/10Mdx (dystrophic). The sample was divided in six groups containing 4 animals each, as: C30 = 30 days control; D30 = Dystrophic with 30 days; C60 = 60 days control; D60 = Distrophic with 60 days; CS60 = 60 days control supplemented with ascorbic acid and DS60 = Dystrophic with 60 days supplemented with ascorbic acid. The ascorbic acid supplementation was given in the water, 0,005 mg/day. After euthanasia, the testicles (right and left) were collected, weighted and cross sectioned. The material was fixed in the Karnovsky solution for 24 hours, included in resin for histological studies (morphological and morphometric analyzes) submitted to ultrastructural analysis and immunohistochemistry for caspase-3. There was a significant increase in the tunica propria percentage in D30 compared to C30 and D60. The ultrastructural analysis showed mitochondrial apoptosis evidence of Sertoli cells that can reduce sperm efficiency in CS60 and DS60. A higher volume density of apoptotic cells postivas to Caspase-3 in C30 and D30 versus DS60 compared to CS60. There was severe hypertrophy of the Leydig cells between D30 and D60. However, with supplementation was observed reversal of this change in DS60. The ultrastructure of Leydig cells to early presence of lipid vesicles was observed in the group pre-pubertal dystrophic (D30). Thus, the DMD affect the organization of the seminiferous tubules and intertubule, however, the ascorbic acid supplementation used for the treatment of DMD has been just enough to reduce the hypertrophy of the Leydig cells.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-04-26T22:18:34Z No. of bitstreams: 1 GlauciaMoisesMarquesESilva_DISSERT.pdf: 4491334 bytes, checksum: ab12f2e9fbbbbe51fff172ebec8580f4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-29T23:53:54Z (GMT) No. of bitstreams: 1 GlauciaMoisesMarquesESilva_DISSERT.pdf: 4491334 bytes, checksum: ab12f2e9fbbbbe51fff172ebec8580f4 (MD5) / Made available in DSpace on 2016-04-29T23:53:54Z (GMT). No. of bitstreams: 1 GlauciaMoisesMarquesESilva_DISSERT.pdf: 4491334 bytes, checksum: ab12f2e9fbbbbe51fff172ebec8580f4 (MD5) Previous issue date: 2015-06-30 / Neste estudo foram utilizados 07 exemplares de Cichlasoma orientale, com objetivo de caracterizar morfohistologicamente o trato digest?rio dessa esp?cie. A cavidade bucofaringeana deste peixe foi investigada por meio de lupa e microsc?pio eletr?nica de varredura e exibiu caracter?sticas como boca terminal com protractibilidade, l?bios liso sendo o superior de menor espessura que o l?bio inferior, a l?ngua triangular e pigmentada, maxila arredondada com pregas palatinas, valva respirat?ria e placas dent?geras com dente c?nico. Na faringe evidencia-se a presen?a de duas placas dent?geras com dent?culos c?nicos, os rastros se apresentam curtos, apenas no primeiro arco braquial e exclusivamente presentes em esp?cimes jovens. As t?cnicas de colora??o HE-Hematoxilina de Harris, Eosina e Peri?dico Schiff foram utilizadas para caracterizar morfologicamente as camadas do tubo digest?rio com auxilio do microsc?pio optico. O es?fago ? curto com pregas na mucosa revestidas por epit?lio pseudoestratificado com c?lulas mucosecretora que se intercalam com c?lulas n?o secret?rias. A camada muscular esof?gica ? espessa com predom?nio de fibras de m?sculo esquel?tico estriado que pode ser evidenciado inclusive pr?ximo ao est?mago. Estas caracter?sticas, provavelmente est?o envolvidas ao h?bito de deglutir presas maiores e prote??o. O est?mago tem formato em Y possuindo tr?s regi?es, todas revestidas por epit?lio simples e provavelmente absortivo. A c?rdica, sem camada muscular da mucosa, com l?mina pr?pria glandular; a f?ndica com muitas pregas mucosas, delgada muscular da mucosa e grande quantidade de gl?ndulas g?stricas; por fim a regi?o pil?rica ? tamb?m glandular e a camada muscular mais espessa quando comparadas as outras regi?es. As caracter?sticas morfol?gicas estomacais indicam um est?mago especializado na digest?o qu?mica, ?cida e proteica. O intestino apresenta-se, anatomicamente, curto com duas al?as intestinais e com tr?s regi?es anterior, m?dia e posterior bem distintas. Quanto ? camada da mucosa, o epit?lio por todo o intestino, tem revestimento do tipo cil?ndrico simples observando-se c?lulas caliciformes e enter?citos, vilosidades tamb?m est?o presentes. O ?rg?o ? totalmente aglandular e provavelmente respons?vel, mais intensamente com a absor??o sendo a digest?o intestinal possivelmente dependente de secre??es hepatopancre?ticas. A morfologia identificada em Cichlasoma orientale apresenta ?rg?os adaptados ao habito alimentar on?voro com tend?ncia alimentar ? carnivoria. / This study used 07 specimens of Cichlasoma orientale, in order to characterize morfohistologicamente the digestive tract of this species. The bucofaringeana cavity of this fish was investigated by means of magnifying glass and scanning electron microscope and exhibited characteristics as terminal mouth with protractibilidade, smooth lips and the upper thinner than the lower lip, triangular and pigmented tongue, rounded jaw with palatine folds, breathing valve and dentigerous plates with conical teeth. In the pharynx highlights the presence of two plates with tapered indentations dentigerous, the tracks are shown shorter, only the first brachial arch and exclusively present in young animals. Technical HEHarris hematoxylin staining, eosin and Periodic Schiff were used to characterize morphologically the layers of the digestive tract with the aid of optical microscope. The esophagus is short with folds mucosa lined by pseudostratified epithelium with mucosecretora cells that are interspersed with nonsecretory cells. The esophageal muscle layer is thick with predominance of striated skeletal muscle fibers which can be evidenced even close to the stomach. These features are probably involved the swallowing habit largest and protection prey. The stomach has format in Y having three regions, all overlaid by a simple epithelium and probably absorptive. The cardial without muscular layer of mucosa with glandular lamina propria; the fundus with many folds mucosa, muscular thin mucous and lot of gastric glands; Finally the pyloric gland and is also thicker muscle layer when compared to other regions. The stomach morphological characteristics indicate a specialized stomach chemistry, acid and protein digestion. The intestine presents itself, anatomically, with two short bowel loops with three anterior, middle and posterior distinct. The layer of the mucosa, the intestinal epithelium throughout, it has a single cylindrical type coating observing goblet cells and enterocytes, villi are also present. The Board is fully responsible aglandular and probably more intensely with the absorption and intestinal digestion possibly dependent hepatopancre?ticas secretions. The morphology identified in Cichlasoma orientale presents organs adapted to the omnivorous eating habits to feed the carnivorous trend.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-12T17:50:11Z No. of bitstreams: 1 VictorPedro_DISSERT.pdf: 1715692 bytes, checksum: 8743cd75cd83a70845cf59f1359a71cf (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-20T18:39:05Z (GMT) No. of bitstreams: 1 VictorPedro_DISSERT.pdf: 1715692 bytes, checksum: 8743cd75cd83a70845cf59f1359a71cf (MD5) / Made available in DSpace on 2016-07-20T18:39:05Z (GMT). No. of bitstreams: 1 VictorPedro_DISSERT.pdf: 1715692 bytes, checksum: 8743cd75cd83a70845cf59f1359a71cf (MD5) Previous issue date: 2015-12-16 / a colite ulcerativa ? uma doen?a cr?nica caracterizada por umainflama??o na mucosa do intestino, na maioria dos casos acomete ? regi?o do c?lon e reto.Na terap?utica s?o utilizados f?rmacos como os aminossalicilatos e glicocorticoster?ides,mas em fun??o da baixa resposta e aos diversos efeitos colaterais promovidos por estes,revela a necessidade da busca de novas fontes de compostos ?teis no tratamento destadoen?a. As diversas partes da planta Anacardium occidentale Linn vem sendo utilizadas as?culos na medicina popular no auxilio de cicatriza??o de les?es de pele e mucosa, estudosrecentes comprovam o seu expressivo efeito antiulcerogenico. OBJETIVO: avaliar oefeito do extrato das folhas de Anacardium occidentale em ratos submetidos ? coliteulcerativa aguda. METODOLOGIA: foram utilizados 42 ratos Wistar machos,distribu?dos em 06 grupos, sendo Controle Negativo (C-), Controle Positivo (C+), Tratadocom Sulfassalazina (CS500) e Tratado com Extrato de A. occidentale nas doses de 50(Ao50), 100 (Ao100) e 200 mg/kg (Ao200). Todos os grupos foram submetidos a ColiteUlcerativa experimental, exceto o C-, al?m disto, C- e C+ receberam solu??o salina durante7 dias consecutivos, enquanto os demais grupos receberam seus respectivos tratamentosrespeitando o mesmo regime que os grupos controles. Neste estudo foi realizado diferentestipos de analise (macrosc?pica, histopatologica, morfom?trica e bioqu?mica) do tecidolesionado e caracterizado preliminarmente o extrato de A. ocidentale. RESULTADOS: oextrato ? rico em saponinas e compostos fen?licos, como flavonoides (quercetina ecanferol) e taninos. Os grupos CS500 e Ao100 apresentaram prote??o significativa a danosa lip?deos e prote?nas, dentre os grupos submetidos a colite ulcerativa experimental oAo100 foi o que obteve o menor escore em todos os parametros analisados.CONCLUS?O: o tratamento com 100 mg/kg de extrato de A. occidentale possibilitou umamelhor recupera??o dos animais, sendo mais eficiente que o tratamento com 500 mg/kg deSulfassalazina, provavelmente devido a combina??o do efeito anti-inflamat?rio,antioxidante, bactericida e anab?lico promovido pelos compostos bioativos presentes noextrato. O tratamento com 50 mg/kg de extrato parece n?o surtir efeito terap?uticosignificante, enquanto que o tratamento com 200 mg/kg do extrato, embora tenhaapresentado melhora significativa dos animais com colite ulcerativa, foi observado umposs?vel efeitos toxico t?pico exercido pelos compostos bioativos em excesso no extrato. / Ulcerative colitis is a chronic disease characterized by inflammation in the intestinal mucosa, in most cases affects the colon and rectum. The therapeutic drugs are used as aminosalicylates and glucocorticosteroids, but due to the low response and the various side effects caused by them, reveals the need to search for new sources of useful compounds in the treatment of this disease.The species Anacardium occidentale popularly known as cashew, has been used for centuries in folk medicine in the healing aid of skin and mucosa lesions.Recent studies show its expressive antiulcerogenic effect, what we instigated to assess the effect of the extract of A. occidentaleleaves in rats with acute ulcerative colitis, therefore, 42 rats were used male Wistar, divided into 06 groups, and Negative Control (C) Positive Control (C +), treated with Sulfasalazine (Sz500) and treated with Extract A. occidentale at doses of 50 (Ao50), 100 (Ao100) and 200 mg / kg (Ao200).All groups were submitted to experimental colitis Ulcerative except C-, moreover, C- and C + received saline via gavage for 7 consecutive days while the other groups received their respective treatments.Euthanasia of animals took place on the 8th day in which it was collected intestinal colon sample for later analysis macroscopic, histopathological, morphometric and biochemistry, as well as complementary collection of blood and liver tissue. The extract is rich in saponins and phenolic compounds such as flavonoids (quercetin and kaempferol) and tannins.When the Sz500 groups and 100 showed significant protection to damage to lipids and proteins, among the groups subjected to experimental ulcerative colitis, the animals Ao100 group obtained the lowest score in all parameters analyzed.Treatment with 100 mg / kg of A. occidentale extract seems to have a combination of antiinflammatory, antioxidant, bactericidal and anabolic promoted by the bioactive compounds present in the extract.However, it is necessary to investigate harder treating dose of 100mg / kg to higher doses compared to elucidate more properly the best therapeutic dosage ulcerative colitis.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-26T20:54:04Z No. of bitstreams: 1 MariaDoSocorroMedeirosAmarante_DISSERT.pdf: 4197812 bytes, checksum: ccd9ecc08e241371d7c86a139df9c8c3 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-08T22:55:59Z (GMT) No. of bitstreams: 1 MariaDoSocorroMedeirosAmarante_DISSERT.pdf: 4197812 bytes, checksum: ccd9ecc08e241371d7c86a139df9c8c3 (MD5) / Made available in DSpace on 2016-08-08T22:55:59Z (GMT). No. of bitstreams: 1 MariaDoSocorroMedeirosAmarante_DISSERT.pdf: 4197812 bytes, checksum: ccd9ecc08e241371d7c86a139df9c8c3 (MD5) Previous issue date: 2015-12-15 / As doen?as inflamat?rias intestinais abrangem um conjunto de desordens cr?nicasinflamat?rias, entre elas a colite ulcerativa (CU). O tratamento da CU se baseia no usode anti-inflamat?rios, por?m estes f?rmacos podem apresentam efeitos colaterais, o queestimula a busca de novas terapias. O objetivo desse trabalho foi avaliar o efeito doextrato hidroalco?lico de Turnera subulata na colite ulcerativa aguda induzida por ?cidoac?tico em ratos. CU foi induzida em ratos Wistar com a instila??o de 1ml de ?cidoac?tico 4% via retal. 42 animais foram distribu?dos em 6 grupos experimentais: GrupoControle, Grupo Col?tico, Grupo Sulfasalazina 500mg/Kg/dia (SSZ), Grupo T. subulata50mg/Kg/dia (TS 50mg), Grupo T. subulata 100mg/Kg/dia (TS 100mg), Grupo T.subulata 200mg/Kg/dia (TS 200mg). Durante o experimento foram avaliadosdiariamente, o consumo alimentar, ingest?o h?drica e peso dos animais. Os animaisforam eutanasiados e o col?n foi exposto para an?lise macrosc?pica. Fragmentos doc?lon foram utilizados para an?lise microsc?pica e avalia??o do estresse oxidativo. Adiferen?a entre as m?dias foi analisada por ANOVA a um n?vel de signific?ncia de 5%(p<0,05) com o aux?lio do Software GraphPad Prism. A an?lise dos resultadosdemonstrou que o Grupo Sulfassalazina teve a maior perda de peso ao longo doexperimento, 14,78% e o menor consumo alimentar de 6,23g de ra??o ao dia, os grupostratados com o extrato de T. subulata n?o apresentaram perda de peso significativaquando comparados com o Grupo Controle. O Grupo Col?tico apresentou o maiorescore macrosc?pico 6,5; enquanto o Grupo TS 50mg teve um menor escore 1,0. Aavalia??o microsc?pica mostrou a presen?a de edema, hemorragia, ulcera??o em todosos grupos experimentais, exceto o Controle. No entanto, o Grupo TS 50mg apresentou oprocesso inflamat?rio em menor intensidade. A avalia??o do estresse oxidativo revelouque os grupos tratados com o extrato de T. subulata modularam a atividade das enzimascatalase e super?xido desmutase. Observou-se ainda redu??o na peroxida??o lip?dica eprot?ica. Assim, ? poss?vel concluir que o extrato hidroalc?olico de T. subulata tevea??o anti- inflamat?ria e antioxidante na colite ulcerativa experimental. / Inflammatory bowel diseases is composed by a set of chronic and inflammatory disorders, among them is ulcerative colitis (UC). UC treatment is based on anti-inflammatory administration; however, this group of drugs clearly leads to development of undesirable side effects, what stimulate the search for new therapies alternatives. The aim of this study was to evaluate the effect of hydroalcholic Turnera subulata extract on acetic acid-induced acute UC in rats. UC was induced by 1 mL injection of 4% acetic acid via rectal in Wistar mouse. 42 animals were distributed among 6 experimental groups: Control, UC, Sulfasalazine 500 mg/Kg/day (SSZ), T. subulata 50mg/Kg/day (TS 50), T. subulata 100mg/Kg/day (TS 100), T. subulata 200mg/Kg/day (TS 200). Throughout the experiment, body weight, food and water ingestion was daily evaluated. At the end of the experiment, the animals were euthanized and a colon fragment was observed by macroscopic analysis. Colon fragments were also collected for microscopic analysis and oxidative stress evaluation. The means from each group was compared by ANOVA test with a significance level of 5% (p<0.05) using GraphPad Prism Software. As results, we can clearly observe that SSZ group had the greater body weight decrease among the groups throughout the experiments, 14.78%, as well as, the lowest food intake, 6.23 g of food/day. The animals treated with T. subulata extracts showed no important body weight loss when compared to control. UC group showed the highest tissue damage macroscope score, 6.5, while TS 50 showed the lowest tissue damage score: 1. Microscope evaluation showed the presence of edema, haemorraghia and ulceration in all group of animals, except for Control. Nevertheless, TS 50 showed the lowest inflammatory damage among all groups. Oxidative stress analysis revealed that T. subulata treatment modulate catalase and superoxide dismutase activity, we also observed a decrease in protein and lipid peroxidation in response to extract administration. Taken together, these results shows that T. subulata extract exerts anti-inflammatory and anti-oxidant effects on experimental UC.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-03-21T18:23:16Z No. of bitstreams: 1 LenildaTeixeiraDaSilva_DISSERT.pdf: 9447586 bytes, checksum: d37926a108960b1f1abce78ad4149e93 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-03-27T21:40:12Z (GMT) No. of bitstreams: 1 LenildaTeixeiraDaSilva_DISSERT.pdf: 9447586 bytes, checksum: d37926a108960b1f1abce78ad4149e93 (MD5) / Made available in DSpace on 2017-03-27T21:40:12Z (GMT). No. of bitstreams: 1 LenildaTeixeiraDaSilva_DISSERT.pdf: 9447586 bytes, checksum: d37926a108960b1f1abce78ad4149e93 (MD5) Previous issue date: 2016-06-21 / A Detritivoria ? comum em peixes dulc?colas, sobretudo na regi?o neotropical, e para muitas esp?cies s?o escassas as informa??es sobre o trato digest?rio, como ? o caso da esp?cie Steindachnerina notonota, na qual, tem import?ncia ecol?gica como elo da cadeia alimentar, servindo de alimento para os peixes carn?voros, e atuando na depura??o dos ecossistemas aqu?ticos sujeitos ? polui??o org?nica. O objetivo do presente estudo consiste em analisar as adapta??es morfol?gicas do trato digest?rio, desta esp?cie end?mica da caatinga, regi?o do semi?rido brasileiro. Assim, foram utilizados 10 esp?cimes adultos (machos e f?meas), necropsiados, para an?lises macrosc?picas e microsc?picas do trato digest?rio. Todas as estruturas foram observadas no estereomicrosc?pio. Os ?rg?os foram fixados em formol 10% e submetidos ? colora??o de Hematoxilina e Eosina, e PAS - ?cido Peri?dico Schiff (rastros branquiais, ?rg?o epibranquial, es?fago, est?mago, intestino m?dio e intestino posterior) e analisados em microscopia de luz. Os rastros branquiais tamb?m foram fixados em paraformalde?do 10% para an?lise em microscopia eletr?nica de varredura. Os esp?cimes analisados possu?am comprimento total entre 6,4 a 13 cm, comprimento padr?o entre 4,8 a 10,3 cm, e peso de 3,23 a 35,92 g. As adapta??es estruturais no trato digest?rio foram: boca do tipo terminal; o particular complexo bucofar?ngeo; aus?ncia de dentes e da l?ngua; tem tr?s tipos de formatos de rastros branquiais com c?lulas mucosas e bot?es gustativos; possui o ?rg?o epibranquial com suas peculiaridades; um est?mago mec?nico com musculatura muito desenvolvida na regi?o pil?rica; e um intestino muito longo e enovelado, que tem internamente uma exclusiva prega helicoidal. Os aspectos morfol?gicos do trato digest?rio de S. notonota t?m rela??o ao h?bito alimentar especializado detrit?voro-ili?fago. / Detritivory is the common freshwater fishes, especially in the Neotropical region, and for many species are scarce information about the digestory tract, as is the case of the species Steindachnerina notonota, in which, it has ecological importance as links in the food chain, serving of feed for carnivorous fishes, and acting in the depuration of aquatic ecosystems subject to organic pollution. The objective of present study consists of analyzing the morphological adaptations of the digestory tract, of this endemic species of the caatinga, Brazilian semiarid region. Thus, 10 adult specimens were used (males and females), necropsied for macroscopic and microscopic analysis of the digestory tract. All structures were observed in the stereomicroscope. The organs were fixed in 10% formalin and submitted to hematoxylin and eosin staining, and PAS - periodic acid Schiff (gill rakers, epibranchial organ, esophagus, stomach, midgut and hindgut) and analyzed by light microscopy. The gill rakers were also fixed in 10% paraformaldehyde for analysis by scanning electron microscopy. The specimens analyzed had total length between 6,4 to 13 cm, standard length between 4,8 to 10,3 cm, and weight of 3,23 to 35,92 g. The structural adaptations in the digestory tract were: mouth of the terminal type; the particular complex bucopharyngeal; absent from tooth and tongue; it has three types of formats of gill rakers with mucous cells and taste buds; possess the epibranchial organ with its peculiarities; a mechanical stomach with highly developed musculature in the pylorus; and a very long and coiled intestine, which internally has exclusive helical fold. The morphological aspects of digestory tract of S. notonota are related to detritivore-ilyophago specialized feeding habit.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-04T18:40:22Z No. of bitstreams: 1 CamillaCarlaDoNascimentoDantasCardoso_DISSERT.pdf: 1584071 bytes, checksum: f40f4e02e45ed4aae3bbf022f745a170 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-12T21:48:51Z (GMT) No. of bitstreams: 1 CamillaCarlaDoNascimentoDantasCardoso_DISSERT.pdf: 1584071 bytes, checksum: f40f4e02e45ed4aae3bbf022f745a170 (MD5) / Made available in DSpace on 2017-04-12T21:48:51Z (GMT). No. of bitstreams: 1 CamillaCarlaDoNascimentoDantasCardoso_DISSERT.pdf: 1584071 bytes, checksum: f40f4e02e45ed4aae3bbf022f745a170 (MD5) Previous issue date: 2016-12-12 / A doen?a inflamat?ria intestinal (DII) engloba um espectro de dist?rbios inflamat?rios cr?nicos e ? classificada em dois subtipos principais: Colite Ulcerativa (UC) e Doen?a de Chron (DC). O tratamento da UC baseia-se no uso de anti-inflamat?rios, mas estes medicamentos na sua forma convencional geram in?meros efeitos adversos, o que estimula o desenvolvimento de pesquisas focadas na busca por novas terapias e tecnologias aplicadas aos f?rmacos. O objetivo deste trabalho foi avaliar a atividade de micropart?culas revestidas por Biopol?meros de quitosana e goma guar contendo como princ?pio ativo a triancinolona, no intuito de observar a resposta anti-inflamat?ria nos par?metros macro e microsc?picos da mucosa intestinal. Foram utilizados 56 ratos Wistar, divididos em 08 grupos de 7 animais, sendo G1: Controle negativo (aus?ncia de colite), G2: Colite, G3: Sulfassalazina (SSZ 500mg/Kg/dia), G4: Triancinolona (TR) livre 5mg/Kg/dia, G5: TR livre 10mg/Kg/dia, G6: TR livre 15mg/Kg/dia, G7: Micropart?cula de TR (15mg/Kg/dia f?rmaco encapsulado) e o grupo G8: Biopol?meros. Os resultados obtidos quanto ao par?metro de avalia??o macrosc?pica mostraram que o menor grau de les?o, com exce??o do grupo Controle negativo, foi encontrado no grupo dos Biopol?meros e SSZ quando comparado aos demais grupos: Colite, TR 5mg, TR 10mg, TR 15mg e Micropart?cula TR. Na an?lise estat?stica desse par?metro os grupos SSZ 500mg/Kg/dia, TR 15mg/kg/dia, Micropart?cula TR 15mg/Kg/dia e Biopol?meros diferiram estatisticamente quando comparadas ao grupo Colite (p<0,05), sendo esta diferen?a mais significativa no grupo dos Biopol?meros (p<0,01). Na an?lise da perda de peso, o grupo Micropart?cula TR reduziu entre 8 a 12% esse par?metro quando comparado aos grupos da Triancinolona livre, diferindo estatisticamente (p<0,05), evidenciando que a tecnologia empregada foi satisfat?ria quanto a redu??o desse efeito adverso. Na avalia??o histopatol?gica o grupo TR 15mg/kg/dia obteve o menor dano tissular, evidenciando o potencial anti-inflamat?rio do f?rmaco livre. Diante dos resultados sugere-se que a nanotecnologia com o uso das micropart?culas foi capaz de reduzir efeitos adversos, como a perda de peso e, al?m disso, obteve resultados com potencial efeito anti-inflamat?rio, apesar da libera??o de apenas 69% dos 15mg da triancinolona encapsulada em 24 horas. Assim, os estudos voltados para aplica??o da nanotecnologia e novos ativos devem crescer e ampliar com intuito de buscar novas terapias e elucidar mecanismos de a??o ainda n?o descobertos. / The inflammatory bowel disease (IBD) encompasses a spectrum of chronic inflammatory disorders and is classified into two main subtypes: Ulcerative Colitis (UC) and Chron's Disease (CD). The treatment of UC is based on the use of anti-inflammatories, but these drugs in their conventional form generate numerous adverse effects, which stimulates the development of research focused on the search for new therapies and technologies applied to the drugs. The objective of this study was to evaluate the activity of microparticles coated by chitosan and guar gum biopolymers containing as active principle triamcinolone in intention to observe the anti-inflammatory response in the macroscopic and microscopic parameters of the intestinal mucosa. In total, 56 Wistar rats divided into 8 groups in 7 animals, being G1: Negative control (absence of colitis), G2: Colitis, G3: Sulfasalazine (SSZ 500mg/Kg/day), G4: Free triamcinolone (TR) 5mg/Kg/day, G5: Free TR 10mg/Kg/day, G6: Free TR 15mg/Kg/day, G7: Triamcinolone microparticle (15mg/Kg/day), G8: Biopolymers. The results obtained for the macroscopic evaluation showed that the lowest lesion degree, except for the negative control group, was found in the Biopolymer group and SSZ group when compared to the other groups: Colitis, TR 5mg, TR 10mg, TR 15mg and TR microparticle. In the statistical analysis of this parameter, the groups treated with SSZ 500mg/kg/day, TR 15mg/kg/day, TR microparticle 15mg/Kg/day and Biopolymers differed statistically when compared to the Colitis group (p<0.05), this difference being more significant in the Biopolymer group (p<0.01). In the analysis of weight loss, the TR microparticle reduced this parameter between 8 and 12% when compared to the free Triamcinolone groups, differing statistically (p<0.05), suggesting that the technology employed was satisfactory in terms of the reduction of this adverse effect. In the histopathological evaluation, the TR group 15mg/kg/day obtained the lowest tissue damage, evidencing the anti-inflammatory potential of the free drug. In view of the results, it is suggested that nanotechnology with the use of microparticles was able to reduce adverse effects, such as weight loss and, in addition, obtained results with a potential anti-inflammatory effect, despite the release of only 69% of 15mg of encapsulated triamcinolone in 24 hours. Thus, studies aimed at the application of nanotechnology and new assets should grow and expand in order to seek new therapies and elucidate mechanisms of action that have not yet been discovered.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-04T18:42:15Z No. of bitstreams: 1 EdsonSoaresDaSilvaJunior_DISSERT.pdf: 3462839 bytes, checksum: 8d21d202beb3732a0d6b66bd9394d7cc (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-12T21:58:14Z (GMT) No. of bitstreams: 1 EdsonSoaresDaSilvaJunior_DISSERT.pdf: 3462839 bytes, checksum: 8d21d202beb3732a0d6b66bd9394d7cc (MD5) / Made available in DSpace on 2017-04-12T21:58:14Z (GMT). No. of bitstreams: 1 EdsonSoaresDaSilvaJunior_DISSERT.pdf: 3462839 bytes, checksum: 8d21d202beb3732a0d6b66bd9394d7cc (MD5) Previous issue date: 2016-12-19 / As cinco esp?cies de tartarugas marinhas que ocorrem no litoral brasileiro est?o classificadas como esp?cies em risco de extin??o pelas listas mundiais de esp?cies amea?adas. Tal status ? decorrente das diferentes amea?as que esses animais v?m sofrendo no decorrer das ?ltimas d?cadas, dentre estas a destrui??o do habitat, fotopolui??o, pesca incidental, tr?fego de ve?culos e preda??o de ovos. Outra consider?vel amea?a a esses animais, descrita na literatura, ? a ocorr?ncia da fibropapilomatose. Esta doen?a acomete as tartarugas marinhas, em especial indiv?duos juvenis da esp?cie Chelonia mydas, embora tenha sido descrito tamb?m nas demais esp?cies. Este trabalho pretende investigar a distribui??o espacial e temporal dos registros de tartarugas marinhas com fibropapilomatose que encalham, vivos ou mortos, entre as praias dos munic?pios de Icapu?/CE at? Cai?ara do Norte/RN, bem como analisar os aspectos histopatol?gicos das les?es. Para isso foram realizados monitoramentos di?rios, utilizando-se quadriciclos, a fim de registrar a ocorr?ncia de tartarugas marinhas nas praias da Bacia Potiguar RN / CE. Os animais que foram encontrados mortos, dependendo do estado da carca?a, foram avaliados quanto ? esp?cie, ao n?mero e posicionamento dos tumores, fotografados e georreferenciados. Os animais vivos foram resgatados e translocados para a Base de Reabilita??o do Projeto Cet?ceos da Costa Branca/UERN-Areia Branca onde foi realizado o procedimento de contagem e localiza??o dos tumores. Para o desenvolvimento da pesquisa est?o sendo utilizados dados coletados entre os anos de 2011 e 2015. Dentre as 3.960 tartarugas marinhas encalhadas, 680 apresentaram tumores sugestivos de fibropapilomatose, valores estes que apresentaram crescimento durante os anos estudados. No que diz respeito a varia??o por trecho de monitoramento, a maior frequ?ncia de animais acometidos pela doen?a foi registrada no trecho C correspondente a "Guamar?-Macau?. Quanto a fase de desenvolvimento, a maior parte dos indiv?duos acometidos estavam no est?gio juvenil, por?m tamb?m houve registro de animais adultos e subadultos acometidos pela doen?a. As les?es se configuram por apresentarem hiperplasia da epiderme, do conjuntivo, desorganiza??o de fibras col?genas, hiperqueratiniza??o de regi?es dentro do tecido conjuntivo e vacuoliza??o no citoplasma. Al?m disso, foram observados ovos de parasitas presentes dentro da estrutura do fibropapiloma, al?m das proje??es papilares que marcam de maneira peculiar a estrutura tumoral. A Bacia Potiguar RN / CE mostrou-se como sendo um trecho com caracter?sticas compartilhadas por outras ?reas de registros da fibropapilomatose e com fatores que podem ter contribu?do para o aumento dos registros de animais acometidos. Por se tratar de uma doen?a debilitante, que acomete principalmente animais juvenis e com etiologia ainda n?o bem definida, a fibropapilomatose impulsiona pesquisas que visam melhor entendimento da doen?a para conserva??o das esp?cies de tartarugas marinhas. / The five species of sea turtles that occur in the brazilian coast are classified as species at risk of extinction by global lists of threatened species. Such status is due to the different threats that these animals have suffered over the past decades, among them the destruction of natural habitat, fotopolui??o, incidental fishing, vehicle traffic and predation of eggs. Another significant threat to these animals, described in the literature, is the occurrence of fibropapillomatosis. This disease affects sea turtles, especially juveniles of the species Chelonia mydas, although it was also described in other species. This work intends to investigate the spatial and temporal distribution of records of sea turtles with fibropapilamatose stranding, living or dead, from the beaches of the municipalities of Icapu? / CE by Cai?ara do Norte / RN, and to characterize the histology of these lesions. For this daily monitoring were performed, using quads in order to record the occurrence of sea turtles in the Bacia Potiguar RN / CE of beaches. Dead animals, depending of the decomposition state, they were evaluated for the type, number and positioning of tumors, photographed and georeferenced. The live animals were rescued and translocated to the rehabilitation base of the project Cet?ceos da Costa Branca / UERN-Areia Branca where we performed the count procedure and location of the tumors. For the development of the research are being used data collected between 2011 and 2015. Among the 3.960 stranded sea turtles, 680 had tumors caused by fibropapillomatosis, values which grew during the years studied. In relation to the variation between the monitored sectors, the highest frequency of animals affected by the disease was recorded in the sector ?C? "Guamar?-Macau." As the development phase, most of the affected individuals were in the immature stage, but there were also adult animals record and subadult affected by the disease. The lesions are configured for presenting papillary projections and epidermal hyperplasia, hyperkeratinisation regions within the connective tissue and points with suggestive of inflammation erythrocyte clusters. In addition, we observed parasites and leech eggs present within tumor structure. The Bacia Potiguar RN / CE has been shown to be an excerpt with features shared by other areas of fibropapillomatosis occurrence and with factors that may have contributed to the increase of the records of affected animals. Because it is a debilitating disease, which affects mainly juvenile animals with etiology not yet well defined, fibropapillomatosis promotes research aimed at a better understanding of the disease for the conservation of sea turtle species.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-04-04T20:01:36Z No. of bitstreams: 1 RodrigoSerafimDeAraujo_DISSERT.pdf: 1281426 bytes, checksum: 6cdad3aba7595958f61e934a0dd41917 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-04-13T00:16:25Z (GMT) No. of bitstreams: 1 RodrigoSerafimDeAraujo_DISSERT.pdf: 1281426 bytes, checksum: 6cdad3aba7595958f61e934a0dd41917 (MD5) / Made available in DSpace on 2017-04-13T00:16:25Z (GMT). No. of bitstreams: 1 RodrigoSerafimDeAraujo_DISSERT.pdf: 1281426 bytes, checksum: 6cdad3aba7595958f61e934a0dd41917 (MD5) Previous issue date: 2016-12-12 / Considerando-se a import?ncia ecol?gica do morcego Artibeus planirostris e a import?ncia da avalia??o epididim?ria para o entendimento de sua fun??o reprodutiva, objetivou-se compreender os par?metros reprodutivos desta esp?cie a partir da an?lise morfol?gica e morfom?trica do epid?dimo. Foram utilizados 16 animais adultos, coletados durante as esta??es seca (n=08) e chuvosa (n=08) de 2014. As capturas foram realizadas na cidade de Natal-RN (latitude 5?50?33.9?S e longitude 35?12?07.6? W) (autoriza??o SISBIO no 25233-1), utilizando-se redes de neblina. Ap?s anestesia e eutan?sia por perfus?o transcard?aca, foi realizada a coleta dos epid?dimos (autoriza??o CEUA/UFRN no 009/2012), seguido de processamento histol?gico para inclus?o em historesina e an?lises sob microscopia de luz. An?lises morfom?tricas dos componentes do par?nquima epididim?rio foram realizadas a partir da captura de imagens das l?minas histol?gicas, utilizando-se o software Image-Pro Plus. Os resultados foram submetidos ? ANOVA, sendo as m?dias comparadas pelo teste t de Student ao n?vel de signific?ncia de 5%, seguido do p?s-teste de Tukey. Nas duas esta??es analisadas, a avalia??o morfol?gica do epid?dimo revelou que o ?rg?o apresentou-se dividido em 4 regi?es principais: segmento inicial, cabe?a, corpo e cauda. O par?nquima apresentou-se composto predominantemente por t?bulos epididim?rios, sustentados por tecido conjuntivo intertubular. Ao longo das tr?s por??es epididim?rias os t?bulos ocuparam 75,07?2,94% do par?nquima na esta??o chuvosa e 67,23?2,44% na esta??o seca, sendo seus percentuais nas regi?es da cabe?a e corpo significativamente maiores na esta??o chuvosa em rela??o ? esta??o seca. O restante do par?nquima foi representado pela regi?o intertubular, que apresentou redu??o proporcional nas mesmas regi?es entre as esta??es. Os t?bulos foram constitu?dos por epit?lio pseudoestratificado cil?ndrico com estereoc?lios, apoiado sobre uma t?nica pr?pria, e l?men. O epit?lio epididim?rio foi o principal componente do t?bulo epididim?rio, sendo que na regi?o da cauda seu percentual foi maior na esta??o seca (27,58?8,33%) em rela??o ? chuvosa (17,79?4,33%). Este epit?lio apresentou-se composto pelas c?lulas principais (PR), basais (BA), estreitas (ES), halo (HA), claras (CL) e apicais (AP), sendo que a primeira e a ?ltima apresentaram, respectivamente, maior e menor distribui??o em todas as regi?es epididim?rias nas duas esta??es. Tanto na regi?o da cabe?a quanto da cauda, as c?lulas PR foram as mais predominantes no epit?lio epididim?rio na esta??o seca em rela??o ? esta??o chuvosa, enquanto as c?lulas BA foram mais predominantes na esta??o chuvosa, tanto na cabe?a quanto na cauda. As c?lulas AP apresentaram maior distribui??o na cabe?a do epid?dimo durante a esta??o chuvosa, enquanto as c?lulas ES apresentaram maior distribui??o no corpo do epid?dimo na esta??o seca, sendo seu registro nessa por??o epididim?ria constituiu um achado novo. Observou-se aumento gradativo em diversos par?metros da cabe?a em dire??o ? cauda, tais como o di?metro dos t?bulos epididim?rios, do l?men e percentual com espermatozoides e da camada de c?lulas musculares lisas ao redor dos t?bulos epididim?rios, contrastando com a diminui??o na altura do epit?lio. Muitos destes par?metros apresentaram maiores valores na esta??o chuvosa em rela??o ? esta??o seca. Conclui-se que o epid?dimo de A. planirostris apresentou-se, de um modo geral, semelhante ao descrito para outros mam?feros, com destaque para as c?lulas estreitas, registradas pela primeira vez na regi?o do corpo. Foram encontrados espermatozoides no l?men dos t?bulos epididim?rios nas duas esta??es avaliadas, especialmente na regi?o da cauda, mostrando assim um padr?o reprodutivo anual cont?nuo, com picos de reprodu??o na esta??o chuvosa. / Considering the ecological importance of the bat Artibeus planirostris and the importance of the epididymal evaluation for the understanding of its reproductive function, this study aimed to understand the reproductive parameters of this species, from the morphological and morphometric analysis of the epididymis. Sixteen adult animals were collected during the dry season (n = 08) and rainy season (n = 08), in 2014. The captures were taken in the city of Natal-RN (5?50'33.9"S and 35?12'07.6"W) (SISBIO authorization no. 25233-1), using mist nets. After anesthesia and euthanasia by transcardiac perfusion, the epididymis were collected (CEUA/UFRN authorization no. 009/2012), followed by histological processing for embedding in historesin and analysis under light microscopy. Morphometric analyzes of the epididymal parenchyma were performed using images from the histological slides, by the Image-Pro Plus software. The results were submitted to ANOVA, and the means were compared by Student's t-test at a significance level of 5%, followed by Tukey's post-test. At the two analyzed seasons, the morphological evaluation showed that the organ was divided into 4 main regions: initial segment, head, body and tail. The parenchyma was predominantly composed of epididymal tubules, supported by intertubular connective tissue. During the three main epididymal portions, the tubules occupied 75.07 ? 2.94 % of parenchyma in rainy season and 67.23 ? 2.44 % in dry season, and its percentages in the head and body were significantly larger in rainy season compared to dry season. The remaining parenchyma was represented by the intertubular region, which showed proportional reduction in the same regions and seasons. The tubules were composed of cylindrical pseudostratified epithelium with stereocilia, supported by a tunica propria and lumen. The epididymal epithelium was the main component of the epididymal tubule. In the tail region, its percentage was higher in dry season (27.58 ? 8.33%) than in rainy season (17.79 ? 4.33%). This epithelium was composed by main (MA), basal (BA), narrow (NA), halo (HA), clear (CL) and apical (AP) cells. The first and the last ones showed, respectively, the highest and the lowest distributions in all epididymal regions, in both rainy and dry seasons. In the head and tail regions, MA cells were the most predominant in the epididymal epithelium in the dry season in relation to the rainy season, while BA cells were the most predominant in the rainy season, such in head as in tail. AP cells had a higher distribution in epididymis head during the rainy season, while NA cells had a greater distribution in the epididymis body during dry season, being its observation in this epididymal portion a new finding. There was an increase in several morphometrical parameters, from the head to the tail, such as the tubular and luminal diameter and its percentages with spermatozoa, as well the muscle cells surrounding the epididymal tubules, in contrast with a decrease in epithelial high. Several of these parameters showed larger values in the rainy season in relation to the dry season. It can be concluded that the epididymis of A. planirostris was similar to that described for other mammals, and the narrow cells were recorded in the region of the body for the first time. It was found spermatozoa in the lumen of epididymal tubules during the two seasons evaluated, especially in the tail region, showing a continuous annual reproductive pattern, with reproductive peaks in the rainy season.