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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Aspectos cl?nicos e imunol?gicos da infec??o por Leishmania Infantum Chagasi em c?es do Rio Grande do Norte / Paula Vivianne Souza de Queiroz. Natal/RN

Queiroz, Paula Viviane de Sousa 16 November 2011 (has links)
Made available in DSpace on 2014-12-17T14:13:42Z (GMT). No. of bitstreams: 1 PaulaVSQ_TESE.pdf: 2112354 bytes, checksum: 4be2c2ba75cc5941ee80bd6de56cfd71 (MD5) Previous issue date: 2011-11-16 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / No Brasil, os c?es est?o envolvidos no ciclo de transmiss?o da Leishmania infantum chagasi, agente etiol?gico da leishmaniose visceral (LV), constituindose em fonte de infec??o para o vetor e promovendo a dispers?o da doen?a para ?reas n?o end?micas. N?o h? tratamento eficaz para LV canina, como tamb?m n?o h? vacinas eficientes.. Os mecanismos imunol?gicos que regulam a susceptibilidade ou resist?ncia ? infec??o por Leishmania em c?es n?o foram completamente caracterizados. A express?o das subclasses de IgG anti- Leishmania tem sido testada como marcador de susceptibilidade ou resist?ncia e consequente status cl?nico de animais infectados. O estudo teve como objetivo avaliar as caracter?sticas cl?nicas e imunol?gicas da infec??o por Leishmania em c?es que residem em ?reas end?micas para leishmaniose visceral, em ambientes periurbano e rural no Estado do Rio Grande do Norte. A popula??o examinada foi proveniente de ?reas de preval?ncia da infec??o canina e humana no RN, sendo 589 c?es de ?reas periurbanas do litoral leste do Estado, 97 c?es de ?reas rurais do munic?pio de S?o Miguel e 146 c?es soropositivos do CCZ de Natal. Os animais foram submetidos ao exame cl?nico e ao diagn?stico sorol?gico atrav?s do teste ELISA, utilizando os ant?genos rK39 e SLA. As subclasses de imunoglobulinas IgG1 e IgG2 foram determinadas atrav?s do ELISA. C?lulas obtidas de ba?o de c?es infectados foram cultivadas para isolamento, identifica??o e quantifica??o do parasita. Altos ?ndices de infec??o foram encontrados em ?rea periurbana (SLA:44,5%;rK39:8,2%) e rural (SLA:44,3%;rK39:11,3%). C?es adultos e machos predominaram entre os soropositivos nas tr?s popula??es estudadas. Unha grande, ?lceras, alopecia e perda de peso foram os sinais cl?nicos mais freq?entes, embora percentuais acima de 70% de assintom?ticos tenham sido registrados. C?es infectados por Leishmania apresentaram t?tulos mais elevados da imunoglobulina IgG2 em rela??o ? IgG1. C?es oriundos de ?reas periurbanas e rurais apresentaram, respectivamente, taxas de soroconvers?o de: 20,9% e 8,3% durante um per?odo de seis meses. A esp?cie L. i. chagasi foi identificada como agente etiol?gico da infec??o canina no CCZ Natal
2

Avalia??o cl?nica, morfol?gica, hematol?gica, bioqu?mica e biomolecular de c?es naturalmente infectados por Ehrlichia canis e Anaplasma platys / Clinical, morphologic, hematological, biochemist and biomolecular evaluation of naturally infects dogs for Ehrlichia canis and Anaplasma platys

Sousa, Val?ria R?gia Franco 20 February 2006 (has links)
Made available in DSpace on 2016-04-28T20:16:32Z (GMT). No. of bitstreams: 1 2006-Valeria Regia Franco Sousa.pdf: 1545960 bytes, checksum: 07990d273ccfe1ac6c38929e5d3d100c (MD5) Previous issue date: 2006-02-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work had for objective to investigate the infection for Ehrlichia canis and Anaplasma platys in the dogs taken care of in the Veterinarian Hospital of the Universidade Federal de Mato Grosso, through the examination of blood smears and of the PCR, analyzing the clinical and laboratories findings. During the period of May of 2004 the July of 2005, 195 dogs with suggestive clinical signs of ehrlichiosis or had contact with the infection had been examined. The canine cyclic thrombocytopenia, caused for the A. platys, provokes reduction of platelets to each 14 days, without provoking severe signs, but when associated to the infection for E. canis the clinical gravity it increases. In the thirteen dogs taken care of with positive PCR for E. canis diagnosised by the PCR it was possible to verify the diversity of signals, with statistical significant predominance of the hemorrhagic riots. Already in the 195 dogs tested for the examination of blood smears it did not have predominance of no clinical signs. The hematologic findings had also been nonspecific, occurring in such a way anemia, leukopenia and thrombocytopenia, how much normality or increase of the cells. The alterations observed in the analysis biochemist had not been exclusive of the groups with infection. In these groups increase of plasma proteins occurred, with hyperglobulinaemia, without, however to have significant difference, despite this finding being frequent in ehrlichiosis. From the analysis of the PCR it was confirmed that the infection for E. canis and A. platys occur in the dogs taken care of in the Veterinarian Hospital of the UFMT, having to be taken writ of prevention, mainly in the control of the vector, since all the ticks found in the examined dogs had been of species Rhipicephalus sanguineus. / Este trabalho teve por objetivo investigar a infec??o por Ehrlichia canis e Anaplasma platys nos c?es atendidos no Hospital Veterin?rio da Universidade Federal de Mato Grosso, atrav?s das t?cnicas de esfrega?o sang??neo e de PCR, analisando os achados cl?nicos e laboratoriais. Durante o per?odo de maio de 2004 a julho de 2005 foram examinados 195 c?es com sinais cl?nicos sugestivos de erlichiose ou com contactantes com a infec??o. A trombocitopenia canina c?clica, causada pelo A. platys, provoca diminui??o das plaquetas a cada 14 dias, sem provocar sinais severos, mas quando associada ? infec??o por E. canis a gravidade cl?nica aumenta. Nos treze c?es atendidos com PCR positiva para E. canis foi poss?vel verificar a diversidade de sinais, com predomin?ncia estatisticamente significativa dos dist?rbios hemorr?gicos. J? nos 195 c?es testados pela t?cnica de esfrega?o sang??neo n?o houve predom?nio de nenhum sinal cl?nico. Os achados hematol?gicos tamb?m foram inespec?ficos, ocorrendo tanto anemia, leucopenia e trombocitopenia, quanto normalidade ou aumento das c?lulas. As altera??es observadas na an?lise bioqu?mica n?o foram exclusivas dos grupos com infec??o. Nestes grupos ocorreu aumento das prote?nas plasm?ticas, com hiperglobulinemia, sem, no entanto haver diferen?a significativa, apesar deste achado ser freq?ente na erliquiose. A partir da an?lise da PCR confirmou-se que a infec??o por E. canis e A. platys ocorre nos c?es atendidos no Hospital Veterin?rio da UFMT, devendo ser tomadas medidas preventivas, principalmente no controle do vetor, j? que todos os carrapatos encontrados nos c?es examinados foram da esp?cie Rhipicephalus sanguineus.
3

Hipotireoidismo em c?es dermatopatas: aspectos cl?nicolaboratoriais comparados ao exame histopatol?gico da pele. / Hypothyroidism in dogs with skin diseases: clinical and laboratory aspects compared to histological examination.

Teixeira, Roberto dos Santos 31 January 2008 (has links)
Made available in DSpace on 2016-04-28T20:18:29Z (GMT). No. of bitstreams: 1 2008 - Roberto dos Santos Teixeira.pdf: 537086 bytes, checksum: 255e9ad40f6e12e7b8375df64bad6672 (MD5) Previous issue date: 2008-01-31 / This study was held at the Dermatology sector of the veterinarian clinic Animalia Rio de Janeiro RJ, from August 1998 to December 2005, 19,7% of the total amount of dogs treated at the clinic(4107) were guided to the Dermatology practice. A number of 266 (6.5%) animals were diagnosed with hypothyroidism. All 100% of the subjects had skin or coat disorders; moreover, 35.7% also had non cutaneous clinical signs of hypothyroidism. The most common dermatopathies were hair coat disorders (65.8%) and keratinization disorders (46.6%). The more repeated non cutaneous clinical signs in the study were metabolic disorders (19.6%) and reproductive (19.2%). The diagnosis of the disease was confirmed dosing the thyroid hormone and TSH (18%), biopsy and histopathology of the skin (35.3%) also by the association of both procedures (46.7%). Within these last group of animals , the histopathology was considered as the most efficient diagnosis method for hypothyroidism (97.6% / 98.2%) as for the Hormone tests the efficiency of this diagnosis was less expressive: T4 Free (72.7% / 75.8%) e T4 Total (77.8% / 73.9%). A percentage of 68,5% of the animals with hypothyroidism , had 1 or 2 secondary diseases or associated to the endocrinopathy, from which immune mediated disorders (45,9%) were accentuated . 157 of the diagnosed patients were submitted to treatment, with positive results in 93.6% of the cases. The disease was most found in females (57,9%). Dogs from 1 to 8 years old (81,6%) represented the age range of the affected animals. There were 43 breeds of dogs involved in the study, from which Chow Chow, Shar pei and English Cocker Spaniel were considered to have predisposition for the disease. / Este estudo foi realizado no setor de Dermatologia da Cl?nica Veterin?ria Anim?lia Rio de Janeiro RJ, no per?odo de agosto de 1998 a dezembro de 2005. Do total de c?es atendidos na cl?nica (4107), 19,7% foram encaminhados ao setor, e em 266 destes (6,5%) foi diagnosticado hipotireoidismo. Em todos os c?es foram observadas altera??es na pele, enquanto que, 35,7% tinham tamb?m sintomas cl?nicos n?o cut?neos do hipotireoidismo. As dermatopatias mais comuns foram os dist?rbios de pelagem (65,8%) e os dist?rbios de queratiniza??o (46,6%). Dos sinais cl?nico-gerais n?o cut?neos, destacaram-se dist?rbios metab?licos (19,6%) e reprodutivos (19,2%). O diagn?stico de hipotireoidismo foi realizado atrav?s de dosagens dos horm?nios tireoidianos e TSH (18%), pela bi?psia e histopatologia de pele (35,3%) e pela associa??o entre estes m?todos (46,7%). Neste ?ltimo grupo de animais, a histopatologia foi considerada como o m?todo de diagn?stico mais eficaz para o hipotireoidismo (97,6% / 98,2%). Com rela??o ?s dosagens hormonais, a efic?cia para este diagn?stico foi menos expressiva: T4 Livre (72,7% / 75,8%) e T4 Total (77,8% / 73,9%). Dos animais com hipotireoidismo, 68,5% tinham 1 ou 2 doen?as secund?rias ou associadas ? endocrinopatia, das quais destacam-se os processos imunomediados (45,9%). Dos animais hipotireoideos, 157 foram submetidos ao tratamento, com resposta positiva em 93,6%. As f?meas (57,9%) foram mais acometidas. Animais com idades entre 1 a 8 anos (81,6%) representaram a faixa et?ria mais freq?entemente afetadas. Dentre as 43 ra?as de c?es acometidas, foram consideradas predispostas Chow Chow, Shar pei e Cocker Spaniel Ingl?s.
4

Aspectos Cl?nicos e Laboratoriais da Cinomose, Ehrlichiose e Borreliose em C?es (Canis familiaris, Linnaeus, 1758) Naturalmente Infectados. / Laboratory of Clinical Aspects of Canine Distemper, Ehrlichiosis and Borreliosis in Dogs (Canis familiaris, Linnaeus, 1758) Naturally Infected.

Santos, Vania Gomes dos 10 July 2008 (has links)
Made available in DSpace on 2016-04-28T20:18:30Z (GMT). No. of bitstreams: 1 2008 - Vania Gomes dos Santos.pdf: 493126 bytes, checksum: 3cd32670acfcb8f78f94330257390af6 (MD5) Previous issue date: 2008-07-10 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Most infectious and parasitic diseases in dogs, have an initial phase with nonspecific symptoms such as apathy, anorexia and occasionally fever with respiratory, gastrointestinal and nervous abnormalities. Generally these changes are related to infection by canine distemper, Ehrlichia sp and the potential presence of exotic etiological agents such as Borrelia sp. This study aimed to detect the occurrence of the canine distemper virus, antibodies against Ehrlichia sp and homologous antibodies against Borrelia burgdorferi in dogs of the Zoonotic Diseases Control Center Paulo Dacorso Filho, of Rio de Janeiro, Brazil correlating with clinical symptoms and haematological disorders. To conduct the research, blood samples were collected from 38 animals collectively housed in five boxes from a population of 125 dogs without defined race, sex, different ages and with an unknown vaccination history. The presence of a positive animal for the canine distemper virus and the absence of clinical cases indicate that the dogs were naturally immunized or have subclinical infection. The number of positive but asymptomatic animals, for Anaplasma platys and Ehrlichia canis in the morphological analysis of blood smears was high, especially for A. platys indicating a high prevalence of sub-clinical infection. However, there was a high title of antibodies against Ehrlichia sp showing prior exposure to this agent. The high titles of homologous antibodies against Borrelia burgdorferi, indicates prior exposure or crossreaction with the spirochete Borrelia sp. and indicates the presence of the causative agent in the region. / A maioria das enfermidades infecciosas e parasit?rias em c?es apresenta uma fase inicial com sintomas inespec?ficos como apatia, anorexia e ocasionalmente febre evoluindo com altera??es respirat?rias, gastrointestinais e nervosas. Em geral essas altera??es s?o relacionadas com a infec??o por viroses como cinomose, bacterioses como Ehrlichia sp., al?m da potencial presen?a de agentes etiol?gicos ex?ticos como Borrelia sp. O presente estudo teve como objetivos detectar qualitativamente o ant?geno do v?rus da cinomose, avaliar a ocorr?ncia de anticorpos anti-Ehrlichia sp. e anticorpos hom?logos contra Borrelia burgdorferi em c?es acautelados no Centro de Controle de Zoonoses (CCZ) Paulo Dacorso Filho, pertencente ao Munic?pio do Rio de Janeiro, Brasil e correlacionar com sintomas cl?nicos e altera??es hematol?gicas. Para realiza??o da pesquisa, foram coletadas amostras de sangue de 38 animais alojados coletivamente em cinco baias de uma popula??o de 125 c?es sem ra?a definida, machos e f?meas, de diferentes idades e com hist?rico de vacina??o desconhecido. A presen?a de um animal positivo para o v?rus da cinomose e aus?ncia de casos cl?nicos indica que os c?es estavam naturalmente imunizados ou com infec??o subcl?nica. O n?mero de animais positivos, por?m assintom?ticos, para Anaplasma platys e Ehrlichia canis ? an?lise morfol?gica em esfrega?os sang??neos foi elevado, especialmente para A. platys indicando uma alta preval?ncia de infec??o sub-cl?nica. Em contrapartida houve um alto t?tulo de anticorpos anti-Ehrlichia sp. evidenciando exposi??o pr?via a este agente. A elevada presen?a de anticorpos hom?logos anti-Borrelia burgdorferi indica exposi??o pr?via ou rea??o cruzada com espiroqueta do g?nero Borrelia e indica a circula??o do agente etiol?gico na regi?o estudada.
5

Influ?ncia de f?rmacos no sistema-renina-angiotensina do globo ocular de c?es h?gidos: desenvolvimento farmacot?cnico e avalia??o cl?nica / Influence of drugs on the renin-angiotensin system of the ocular globe of healthy dogs: pharmacotechnical development and clinical evaluation

Caminotto, Eriane de Lima 21 October 2015 (has links)
Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-24T16:23:42Z No. of bitstreams: 1 2015 - Eriane de Lima Caminotto.pdf: 1339748 bytes, checksum: 753de2c181b04ca05748c103c2d6ec8b (MD5) / Made available in DSpace on 2017-01-24T16:23:42Z (GMT). No. of bitstreams: 1 2015 - Eriane de Lima Caminotto.pdf: 1339748 bytes, checksum: 753de2c181b04ca05748c103c2d6ec8b (MD5) Previous issue date: 2015-10-21 / Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do RJ - FAPERJ / Glaucoma is a disease that causes more blindness in dogs and has no cure, only treatment. The difficulty in the drainage of aqueous humor (AH) results in an increase in intraocular pressure (IOP), representing a significant risk factor for the occurrence of this condition, leading to irreversible damage to the progressive loss of visual field and vision as a whole death of retinal ganglion cells (RGC), loss of axons in the optic nerve and excavation of the same. In addition to this, several mechanisms may contribute to the development and progression of this disease, for example, unbalances of the renin-angiotensin-system (RAS), and cardiovascular and renal diseases. The measurement of IOP and the examination of the fundus are the methods used to diagnose this condition. The treatment of choice is based on eye drops that stimulate drainage of HA and / or decrease their production resulting in IOP control; however in many cases the treatment does not attain the desired effect, culminating in blindness. In an attempt to change this therapeutic perspective, this study compared, in healthy and normotensive Beagle dogs, systemic and ocular action of three drugs: timolol maleate 0.5% (non-selective beta-blocker used in cases of glaucoma) , captopril 0.1% and 0.5% (ECA1 inhibitor never before studied in greater concentration in dogs) and aceturato of diminazene - DIZE 0.1% and 0.5% (endogenous activator of ACE2, never before researched in eyes canines). The latter two drugs were formulated for ophthalmic use for the reduction of IOP, since it is known about the existence of SARS eye level, and that they have been successfully administered systemically at reducing systemic arterial pressure (SAP). Thus, before and after treatment, 18 dogs underwent the Schirmer Tear Test (TLS1) and blood collection for measuring the concentration of angiotensin-converting enzyme (ECA1) serum. For three consecutive days before treatment and at three times (6h, 12h and 18h) all animals have gone through four exams, always following the same order: evaluation of pupil size, IOP measurement, measurement of HR and SAP. After obtaining the normal range, the animals had the left eye subjected to predetermined protocols initially with 1 drop every 12 hours, 7 days a lower concentration and, in the other seven days, in the highest concentration. The adelfos eyes were control and every day in the three different times dogs went through the same initial exams. All drugs were good penetration and no adverse eye level. As for tear production, captopril is the most suitable for glaucoma patients and patients with keratoconjunctivitis sicca, while timolol is contraindicated for them. All decreased IOP values, and in the second week of treatment, with higher concentrations, reductions were more significant. The bradycardia was observed in captopril-treated animals 0.5%, with a reduction of almost 9% in HR compared to captopril in lower concentrations / O glaucoma ? uma das doen?as que mais causa cegueira em c?es e n?o possui cura, apenas tratamento. A dificuldade na drenagem do humor aquoso (HA) resulta no aumento da press?o intra-ocular (PIO) que representa um fator de risco significativo para a ocorr?ncia dessa afec??o, conduzindo ? danos irrevers?veis como a perda progressiva do campo visual e da vis?o como um todo, morte das c?lulas ganglionares da retina (CGR), perda de ax?nios do nervo ?ptico e escava??o do mesmo. Al?m deste, diversos mecanismos podem contribuir para o desenvolvimento e progress?o desta doen?a como, por exemplo, os desequil?brios do sistema-renina-angiotensina (SRA) e as doen?as cardiovasculares e renais. A mensura??o da PIO e o exame do fundo de olho s?o os m?todos mais utilizados para diagnosticar essa afec??o. O tratamento de escolha se baseia em col?rios que estimulam a drenagem do HA e/ou diminuam sua produ??o resultando no controle da PIO; por?m em muitos casos a terapia n?o atinge o efeito desejado e culmina em cegueira. Na tentativa de mudar essa perspectiva terap?utica, o presente trabalho comparou, em c?es sadios e normotensos da ra?a Beagle, a a??o sist?mica e ocular de tr?s f?rmacos: maleato de timolol 0,5% (beta-bloqueador n?o seletivo usado nos casos de glaucoma), captopril 0,1% e 0,5% (inibidor de ECA1 nunca antes estudado nesta maior concentra??o em c?es) e aceturato de diminazeno ? DIZE 0,1% e 0,5% (ativador end?geno de ECA2, nunca antes pesquisado em olhos caninos). Os dois ?ltimos f?rmacos foram formulados para uso oftalmol?gico visando a redu??o da PIO, uma vez que ? sabido sobre a exist?ncia do SRA a n?vel ocular e que os mesmos j? foram administrados sistemicamente com sucesso na redu??o press?o arterial sist?mica (PAS). Dessa forma, antes e ap?s os tratamentos, 18 c?es foram submetidos ao teste lacrimal de Schirmer (TLS1) e ? coleta sangu?nea para dosagem da concentra??o da enzima conversora de angiotensina (ECA 1) do soro.Durante tr?s dias consecutivos antes dos tratamentos e em tr?s hor?rios distintos (6h, 12h, e 18h) todos os animaispassaram por quatro exames, seguindo sempre a mesma ordem: avalia??o do di?metro pupilar, aferi??o da PIO, aferi??o da FC e da PAS.Ap?s a obten??o dos valores normais, os animais tiveram o olho esquerdo submetido aos protocolos pr?-determinados inicialmente com 1 gota, a cada 12 horas, por 7 dias na menor concentra??o e, nos demais 7 dias, na maior concentra??o. Os olhos adelfos foram o controle e, todos os dias nos tr?s hor?rios distintos os c?es passaram pelos mesmos exames iniciais. Todos os f?rmacos tiveram boa penetrabilidade e aus?ncia de efeitos adversos a n?vel ocular. Quanto ? produ??o lacrimal, o captopril ? o mais indicado para os pacientes glaucomatosos e portadores de ceratoconjuntivite seca, enquanto que o timolol ? contra-indicado para os mesmos. Todos diminu?ram os valores da PIO, sendo que na segunda semana de tratamento, com concentra??es maiores, as redu??es foram mais significativas. A bradicardia foi observada nos animais tratados com captopril 0,5%, com uma redu??o de quase 9% na FC, quando comparado com o captopril em menor concentra??o.
6

Avalia??o hematol?gica, bioqu?mica, cl?nica e diagn?stico molecular de agentes micoplasmas hemotr?ficos em c?es dom?sticos oriundos de abrigos da regi?o metropolitana do Rio de Janeiro / Hematological, biochemical, clinical and molecular diagnosis of mycoplasma hemotrophic agents in domestic dogs from shelters in the metropolitan region of Rio de Janeiro

SILVA, Aline Tonussi da 29 February 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-14T18:58:03Z No. of bitstreams: 1 2016 - Aline Tonussi da Silva.pdf: 2072458 bytes, checksum: 143dab8a0068b4bd1a643fe48f82873c (MD5) / Made available in DSpace on 2017-02-14T18:58:03Z (GMT). No. of bitstreams: 1 2016 - Aline Tonussi da Silva.pdf: 2072458 bytes, checksum: 143dab8a0068b4bd1a643fe48f82873c (MD5) Previous issue date: 2016-02-29 / CAPES / The hemotropic hemoparasites mycoplasmas are gram-negative bacteria, which must attach to the wall of erythrocytes, causing deformation in the membrane of the infected erythrocytes. The goal of this study was to detect molecularly hemoplasmas infection in sheltered dogs in Metropolitan region of Rio de Janeiro, linking it to hematological, biochemical and clinical data changes. Of 222 dogs sampled, 20,27% (45/222) were positive by the Polymerase Chain Reaction Real Time (qPCR) for Mycoplasma spp.. The frequency of hemoplasma species infection by PCR using species-specific oligonucleotides, was 13,06% (29/222), 0% (0/222) and 2,25% (5/222) respectively for M. haemocanis (Mhc), ?Candidatus M. turicensis? (Cmt) and ?Candidatus Mycoplasma haematoparvum / haemominutum? (Cmhp / m). There for e M. haemocanis presented the highest incidence in the dogs population studied. Coinfections were identified between Cmhp/m and Mhc 1,35% (3/222). Regarding hematological alterations associated with infection by Mycoplasma spp., hyperproteinemia on plasma was detected (p = 0,0010). It was not observed an association between hemoplasmas infection and the occurrence of anemia and leukocyte changes. Changes in serum biochemistry showed a significant difference in serum protein (p = 0,0291), globulin (p = 0,0253), creatinine (p = 0,0279) and alkaline phosphatase (p = 0,0242). Additionally the azotemia was statistically (p = 0,0023) associated with the infection. The results obtained in the evaluation of Mhc positive dogs demonstrated no significant difference in plasma total protein (p = 0,0012) and hyperproteinaemia was associated with infection (p = 0,0238). There were significant differences in alkaline phosphatase parameter (p = 0,0173) and urea (p = 0,0401), with azotemia is associated shall infection (p = 0,0061). The results observed in the evaluation of positive dogs for (Cmhp / m) show that ALT (alanine aminotransferase) was associated with infection (p = 0,0301). As for factors associated with infection by Mycoplasma spp., only gender (p = 0,0062) and age (p = 0,0041) were associated with infec??o.N?o are statistically associated with the historical agent of fights, access to street, castration, history fleas, ticks history, presence of fleas and ticks. The frequency of the main findings of anamanse and clinical examination of the dogs positive for Mycoplasma spp . were lymphadenopathy (37,8% ) , skin lesions compatible with scabies ( 28,9%) , TPC > 2 ( 31,1% ) , oral affections ( 15,5%) of hist?rico ehrlichiosis ( 13,3%) . Consequently, knowledge of the disease and the factors related to their occurrence are necessary for the adoption of preventive measures aimed at animal health. / Os hemoparasitos micoplasmas hemotr?ficos s?o bact?rias gram-negativas, que se fixam obrigatoriamente na superf?cie dos eritr?citos, levando a deforma??o desta. O objetivo do presente estudo foi detectar molecularmente a infec??o por hemoplasmas em c?es de abrigos da Regi?o Metropolitana do Rio de Janeiro, associando-a aos dados hematol?gicos, bioqu?micos e altera??es cl?nicas. Dos 222 c?es amostrados, 20,27% (45/222) foram positivos pela Rea??o em Cadeia da Polimerase em Tempo Real (qPCR) para Mycoplasma spp.. A frequ?ncia de infec??o das esp?cies de hemoplasma pela PCR, utilizando oligonucleot?deos esp?cie-espec?ficos, foi 13,06% (29/222), 0% (0/222) e 2,25% (5/222), respectivamente para M. haemocanis (Mhc), ?Candidatus M. turicensis (CMt) e ?Candidatus Mycoplasma haematoparvum/ haemominutum? (CMhp/m). M. haemocanis constitui, portanto, a esp?cie de maior ocorr?ncia na popula??o de c?es estudada. Foram identificadas coinfec??es entre CMhp/m e Mhc em 1,35% (3/222) dos c?es. No tocante ?s altera??es hematol?gicas associadas ? infec??o por Mycoplasma spp., constatou-se hiperproteinemia plasm?tica refratom?trica (p=0,0010). N?o foi observada associa??o entre a infec??o por hemoplasmas e a ocorr?ncia de anemia ou altera??es leucocit?rias. Nas altera??es da bioqu?mica sangu?nea, evidenciou-se diferen?a significativa na prote?na s?rica (p=0,0291), globulina (p=0,0253), creatinina (p=0,0279), fosfatase alcalina (p=0,0242). Adicionalmente, a azotemia foi estatisticamente (p=0,0023) associada ? infec??o. Os resultados observados na avalia??o de c?es positivos para Mhc demonstram haver diferen?a significativa na prote?na plasm?tica total (p=0,0012) e hiperproteinemia foi associada ? infec??o (p=0,0238). Houve diferen?a significativa no par?metro fosfatase alcalina (p=0,0173) e ur?ia (p=0,0401), com a azotemia sendo associada ? infec??o (p=0,0061). Os resultados observados na avalia??o de c?es positivos para CMhp/hm demonstram que a ALT (alanina aminotransferase) foi associada ? infec??o (p=0,0301). Quanto aos fatores associados ? infec??o por Mycoplasma spp., apenas g?nero (p=0,0062) e idade (p=0,0041) foram associadas ? infec??o. N?o apresentaram associa??o estat?stica com o agente hist?rico de brigas, acesso a rua, castra??o, hist?rico de pulgas, hist?rico de carrapatos, presen?a de pulgas e presen?a de carrapatos. A frequ?ncia dos principais achados de anamanse e exame cl?nico dos c?es positivos para Mycoplasma spp. foram a linfadenopatia (37,8%), les?es de pele compat?veis com sarna (28,9%), TPC >2 (31,1%), afec??es bucais (15,5%) e hist?rico de erlichiose (13,3%). Consequentemente, o conhecimento da doen?a bem como dos fatores relacionados ? sua ocorr?ncia s?o necess?rios para a ado??o de medidas preventivas visando ? sa?de animal.
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Remiss?o da demodiciose canina ap?s o tratamento com a doramectina em diferentes protocolos / Remission of canine demodicosis after treatment with different protocols of doramectin

FERREIRA, Fabr?cia Ferreira e 23 March 2016 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-06-26T18:12:15Z No. of bitstreams: 1 2016 - Fabr?cia Ferreira e Ferreira.pdf: 1846980 bytes, checksum: c127e17162c67e64b719801f92b0c6fc (MD5) / Made available in DSpace on 2017-06-26T18:12:15Z (GMT). No. of bitstreams: 1 2016 - Fabr?cia Ferreira e Ferreira.pdf: 1846980 bytes, checksum: c127e17162c67e64b719801f92b0c6fc (MD5) Previous issue date: 2016-03-23 / Canine demodicosis is an inflammatory skin disease, frequently diagnosed in veterinary clinics, caused by the proliferation of mites of the species Demodex sp. In recent years, important findings about the disease have been reported, mainly aspects related to treatment, with the insertion of new molecules or new treatment regimens. Doramectin is a macrocyclic lactone that has been used empirically by veterinarians, who use different routes, doses and intervals in its administration, with no homogeneus results. This study aimed to evaluate the use of doramectin in the treatment of dogs affected by the generalized form of demodicosis. Of the forty-six dogs diagnosed with the disease during the study, 20 were selected for the study and divided into three groups: Group I ? treated with doramectin at a dose of 600 mcg/kg once a week orally, group II ? treated at a dose of 300 mcg/kg orally every 3 days and group III ? treated at a dose of 600 mcg/kg every 7 days subcutaneously. The animals were treated until three consecutive negative skin scrapings were obtained, with intervals of at least fifteen days between them (parasitological cure). The days required to obtain the parasitological cure were 105, 82 and 100 according to the indicated groups; and their treatment efficiencies were 75, 100 and 83%, respectively. Doramectin was effective in treating generalized demodectic mange in dogs, regardless of the dose, route and interval of administration. However, the best results were obtained in the group treated at a dose of 300 mcg/kg orally every 3 days. There were no reported adverse reactions with the use of macrocyclic lactone. / Demodiciose canina ? uma doen?a inflamat?ria da pele, frequentemente diagnosticada nos consult?rios veterin?rios, causada pela prolifera??o de ?caros da esp?cie Demodex sp. Nos ?ltimos anos, importantes descobertas sobre a doen?a foram reportadas, principalmente os aspectos relacionados ao tratamento, com a inser??o de novas mol?culas ou novos esquemas de tratamento. A doramectina ? uma lactona macroc?clica que vem sendo usada de forma emp?rica por m?dicos veterin?rios, que a utilizam por diferentes vias, doses e intervalos na sua administra??o, com resultados heterog?neos. O objetivo do estudo foi avaliar a utiliza??o da doramectina no tratamento da demodiciose generalizada em c?es. Dos 46 animais diagnosticados com a doen?a, 20 foram selecionados e divididos em tr?s grupos experimentais: grupo I ? tratado com doramectina dose de 600 mcg/kg semanalmente por via oral, grupo II ? tratado na dose de 300 mcg/ kg por via oral a cada 3 dias e o grupo III ? tratado na dose de 600 mcg/kg a cada 7 dias por via subcut?nea. Os animais foram tratados at? a obten??o de tr?s raspados negativos consecutivos com pelo menos 15 dias de intervalo entre eles (cura parasitol?gica). Os dias necess?rios para obten??o da cura parasitol?gica foram 105, 82 e 100 de acordo com os grupos assinalados e as respectivas efic?cias ao tratamento foram 75, 100 e 83%. A doramectina demonstrou ser eficaz no tratamento da demodiciose generalizada em c?es independente da dose, via e intervalo de sua administra??o. Entretanto, os melhores resultados obtidos foram observados no grupo tratado com a dose de 300 mcg/ kg por via oral a cada 3 dias. N?o foram reportadas quaisquer rea??es adversas com a utiliza??o da lactona macroc?clica.
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Diagn?stico molecular e avalia??o hematol?gica de micoplasmas hemotr?ficos em c?es dom?sticos (Canis familiaris) nas ?reas urbana e rural de Aragua?na, Tocantins, Brasil / Molecular diagnostic of haemotropic parasites of dogs (Canis familiaris) under natural conditions of urban and rural areas of Aragua?na, state of Tocantins, Brazil

BOTELHO, Camila Fl?via Magalh?es 14 February 2017 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-10-04T18:07:36Z No. of bitstreams: 1 2017 - Camila Fl?via Magalh?es Botelho.pdf: 1531668 bytes, checksum: b445ba2353dc7515e478a8e151b24d75 (MD5) / Made available in DSpace on 2017-10-04T18:07:36Z (GMT). No. of bitstreams: 1 2017 - Camila Fl?via Magalh?es Botelho.pdf: 1531668 bytes, checksum: b445ba2353dc7515e478a8e151b24d75 (MD5) Previous issue date: 2017-02-14 / CAPES / Hemotrophic mycoplasmas hemoparasites are pleomorphic, epicellular, gram negative organisms that are located on the surface of erythrocytes of several species. In dogs, hemoplasma infection can cause hemolytic anemia in the acute phase, while in the chronic phase the signs are generally inapparent, and immunosuppression can trigger the acute disease. The present study aimed to detect molecularly the presence of hemotrophic mycoplasma agents in domestic dogs from the urban and rural areas of Aragua?na, Tocantins and, in addition, to correlate hematological alterations and factors associated with natural infection. In the analysis of the results, 2.9% (3/105) of the dogs sampled from the urban area and 24.49% (24/99) from the rural area were positive for Mycoplasma spp. by the Real Time Polymerase Chain Reaction (qPCR). The frequency of hemoplasmas species infection by PCR using species-specific oligonucleotides was 1.9% (2/105) and 13.26% (13/99) for Mycoplasma haemocanis in the urban and rural areas, respectively. No positive dogs were observed for ?Candidatus Mycoplasma turicensis? and ?Candidatus Mycoplasma haematoparvum?/?Candidatus Mycoplasma haemominutum? in both areas studied. Regarding hematological alterations, there was no statistical association with the infection by Mycoplasma spp. Similarly, among the analyzed variables (age, gender, racial pattern, site cleanliness and housing), no statistical correlation was observed in either urban or rural areas. This is the first report of the molecular detection of Mycoplasma haemocanis in dogs from Tocantins and opens up prospects for future studies in this group of hemotrophic agents. / Os hemoparasitos micoplasmas hemotr?ficos s?o organismos pleom?rficos, epicelulares, Gram negativos, que se localizam na superf?cie dos eritr?citos de diversas esp?cies. Em c?es, a infec??o pelos hemoplasmas pode causar anemia hemol?tica na fase aguda, enquanto na doen?a cr?nica os sinais em geral s?o inaparentes, sendo que a imunossupress?o pode desencadear a doen?a aguda. O presente estudo objetivou detectar molecularmente a presen?a de infec??o pelos agentes micoplasmas hemotr?ficos em c?es dom?sticos procedentes de ?reas urbana e rural de Aragua?na, Tocantins e, adicionalmente correlacionar as altera??es hematol?gicas e fatores associados com a infec??o natural. Na an?lise dos resultados obtidos, 2,9% (3/105) dos c?es amostrados da ?rea urbana e 24,49% (24/99) da ?rea rural foram positivos pela Rea??o em Cadeia da Polimerase em Tempo Real (qPCR) para Mycoplasma spp. A frequ?ncia de infec??o das esp?cies de hemoplasmas pela PCR, utilizando oligonucleot?deos esp?cie-espec?ficos, foi de 1,9 % (2/105) e 13,26% (13/99) para Mycoplasma haemocanis na ?rea urbana e rural, respectivamente. N?o foram observados c?es positivos para ?Candidatus Mycoplasma turicensis? e ?Candidatus Mycoplasma haematoparvum?/?Candidatus Mycoplasma haemominutum? em ambas as ?reas estudadas. No tocante ?s altera??es hematol?gicas, n?o houve associa??o estat?stica com a infec??o por Mycoplasma spp. De modo semelhante, dentre as vari?veis analisadas (idade, sexo, padr?o racial, limpeza do local e moradia) n?o foi verificada qualquer correla??o estat?stica com a infec??o pelo agente Mycoplasma spp., seja na ?rea urbana ou na rural. Esta constitui a primeira detec??o molecular de Mycoplasma haemocanis em c?es do Tocantins e abre perspectivas para futuros estudos neste grupo de agentes hemotr?ficos.
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Avalia??o cl?nica e laboratorial do tratamento com lactulose de c?es com doen?a renal cr?nica / Clinical and laboratorial evaluation of the treatment with lactulosis of dogs with chronic kidney disease

PEREIRA, Juliana de Abreu 27 April 2017 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-03-15T19:04:01Z No. of bitstreams: 1 2017 - Juliana de Abreu Pereira.pdf: 2216195 bytes, checksum: 788fd1f8f4db4f3012004d0eec09d439 (MD5) / Made available in DSpace on 2018-03-15T19:04:01Z (GMT). No. of bitstreams: 1 2017 - Juliana de Abreu Pereira.pdf: 2216195 bytes, checksum: 788fd1f8f4db4f3012004d0eec09d439 (MD5) Previous issue date: 2017-04-27 / CAPES / Prebiotics, such as lactulosis, may favor the switch on the fermentative pattern of the colonic microbiota from proteolytic to saccharolytic, which allows bigger assimilation of nitrogenous compounds by the microrganisms of the colon. The present study aimed to evaluate, in dogs with CKD, the effect of the continued orally use of lactulosis over the nitrogenous compounds metabolisms?, the iron metabolism and on serum levels of albumin, magnesium, calcium and phosphorus. Twenty-one animals with CKD in IRIS II and III stages, under normal handling and feeding, were clinically and laboratorially evaluated by a 28 days period; divided in three groups according to treatment: T1 ? lactulosis + therapeutic diet, T2 ? lactulosis + standard treatment + therapeutic diet, T3 ? standard treatment + therapeutic diet . For the three groups (T1, T2 and T3), clinical parameters indicated anaemia and body score from regular to bad, according to the disease?s degree, during the hole treatment. The haematological and biochemical?s averages are consistent with common laboratorial findings in nephrophatic patients, with high levels of urea and creatinine; and low leves of haematocrit. For all the evaluated parameters in this study, the averages? variations during the period didn?t show any significant difference between times and treatments, with the exception of the calcemia averages that were greater for T1 group; which may indicate that for this animals? group the monotherapy with lactulosis could have increased the absorption of this mineral in those patients. With regard to iron metabolism, this study?s data revealed that the anaemia found in the dogs throughout the experimental period presented chronicity features, since the groups? means remained within the iron and transferrin references, besides high ferritin averages, without significant differences between groups and moments. The obtained data allowed to conclude that there was no difference between the proposed treatments in relation to clinical state and the biochemical and mineral profiles, such as iron metabolism; which justifies that the action mechanisms of prebiotics in nephrophatic patients should be evaluated with more details. / Prebi?ticos, como a lactulose, podem favorecer a altera??o do padr?o fermentativo da microbiota col?nica de proteol?tico para sacarol?tico; o que possibilita maior assimila??o de compostos nitrogenados pelos microrganismos do c?lon. O presente estudo teve por objetivo avaliar, em c?es com DRC, o efeito da utiliza??o continuada de lactulose por via oral, sobre o metabolismo de compostos nitrogenados; o metabolismo do ferro, e sobre as concentra??es s?ricas de albumina, magn?sio, c?lcio e f?sforo. Vinte e um animais portadores de DRC em est?gios IRIS II e III, com manejo e alimenta??o normais, foram avaliados clinicamente e laboratorialmente por um per?odo de 28 dias; divididos em tr?s grupos conforme o tratamento: T1 ? lactulose + ra??o terap?utica, T2 ? lactulose + tratamento convencional + ra??o terap?utica, T3 ? tratamento convencional + ra??o terap?utica. Para os tr?s grupos (T1, T2 e T3), os par?metros cl?nicos foram indicativos de anemia e escore corporal de regular a ruim, de acordo com o grau da enfermidade, ao longo de todo tratamento. As m?dias dos par?metros hematol?gicos e bioqu?micos s?o condizentes com achados laboratoriais comuns em nefropatas; com elevados valores de ureia e creatinina e valores decrescidos de hemat?crito. Para todos os par?metros as varia??es durante o per?odo n?o apresentaram diferen?a significativa entre os tempos e tratamentos, ? exce??o das m?dias de calcemia que foram maiores para o grupo T1; o que pode indicar que para os animais deste grupo a monoterapia com lactulose pode ter aumentado a absor??o deste mineral. Com rela??o ao metabolismo de ferro, os dados revelaram que a anemia encontrada nos c?es em todo o per?odo experimental apresentou caracter?sticas de cronicidade, uma vez que as m?dias dos grupos permaneceram dentro dos intervalos de refer?ncia para ferro e transferrina; al?m de m?dias elevadas de ferritina, sem diferen?as significativas entre grupos e momentos. Os dados obtidos permitiram concluir que n?o houve diferen?a entre os tratamentos propostos com rela??o ao estado cl?nico e aos perfis bioqu?micos e minerais, bem como ao metabolismo de ferro; o que justifica que os mecanismos de a??o dos prebi?ticos em nefropatas devem ser avaliados com maiores detalhes.
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Preval?ncia de Parasitos Zoon?ticos em Solos e Fezes de Pra?as P?blicas Segundo Testes Diagn?sticos, 2006 / Prevalence of zoonotic parasites in soil of public places according to diagnostic tests. Seropedica, State of Rio de Janeiro, 2006.

Pereira, Arisa Mandarino 23 February 2007 (has links)
Made available in DSpace on 2016-04-28T20:15:27Z (GMT). No. of bitstreams: 1 2007 - Arisa Mandarino Pereira.pdf: 822408 bytes, checksum: 5607ce9cfb143ed2b5233a56791a74cb (MD5) Previous issue date: 2007-02-23 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The aim of this study was to investigate the prevalence of zoonotics parasites of dogs and cats in soil of public squares at Serop?dica City in state of the Rio de Janeiro according to diagnostic tests. All 25 registered squares of this city participated in this study being visited at least once. Considering the soil area as rectangular, five soil samples were collected, with about 250g each one. All fresh fecal samples of dogs and cats found during the visits were collected. In total were 125 soil samples submitted to Dunsmore et al. s technique (1984) and Adaptation of Rugai s methods (CARVALHO et al., 2005) and 81 fecal samples were analyzed according to Willis', Hoffman s and centrifuge- flotation techniques. The soil and feces samples were processed and analyzed at the laboratory of Cocc?dios e Coccidioses PSA (EMBRAPA/UFRRJ). The ?2 test with 5% of significance was made in Epinfo program 2002 to evaluate the association between parasites prevalence and diagnostic tests used. Eggs of Ancylostomatids, Toxocarids, Trichuris spp., Ascaris spp. and larvae of Ancylostomatids and Strongyloides spp. were detected in soil of seven (28.00%) squares. The Adaptation of the of Rugai s method (CARVALHO et al., 2005), to the soil s analysis, is more efficient than the Dunsmore et al. s technique (1984) because is cheaper and easier to executed besides detecting eggs of Ancylostomatids (4.80%), Ascaris spp. (1.60%), Trichuris spp. (2.40%), larvae of Ancylostomatids (8.80%) and Strongyloides spp. (1.60%) and free living nematodes (36.80%), forms that were not detected by the Dunsmore et al. s technique (1984). The prevalence of Toxocarids eggs was similar (p= 0.213) in both techniques. In 92.50% of the 81 fecal samples collected was observed at least a parasite type in one of the technique use. The most prevalent parasite in animals` feces was Ancylostomatids (80.25%) following for Toxocarids (11.11%), cestoids (8.64%), Cryptosporidium spp. (7.41%) and Trichuris spp. (6.17%). In 34.57% fecal were observed multiple infections by two or three different types of parasites. The low prevalence of Ancylostomatids eggs in soil samples contrast with the high prevalence observed in feces, suggesting that the environment conditions are not favourable to the development and survival of its infectants forms, although it can be present in fewer amount than those capable by the techniques used. The low prevalence of Toxocarids in feces in comparison of Ancylostomatids, indicated that animals, which have access to those places, are adults and not litter, which are the main responsible to spread Toxocarids eggs in the environment. Although have been identified in feces, any cyst or protozoan oocyst was diagnosed in soil sample indicating the necessity of diagnostic techniques efficient enough to detect such parasites in soil. / Este estudo foi realizado com o objetivo de investigar a preval?ncia de parasitos zoon?ticos de c?es e gatos em solo de pra?as p?blicas do Munic?pio de Serop?dica segundo t?cnicas de diagn?stico. Todas as 25 pra?as registradas no munic?pio foram inclu?das no estudo sendo visitadas uma ?nica vez. Considerando a ?rea de solo como retangular, foram coletadas cinco amostras de solo com cerca de 250g cada uma. Todas as amostras fecais frescas de c?es e gatos encontradas no momento da visita foram coletadas. Ao total foram 125 amostras de solo submetidas ?s t?cnicas de Dunsmore et al. (1984) e a de Adapta??o do m?todo de Rugai (CARVALHO, et al., 2005) e 81 amostras fecais analisadas segundo as t?cnicas de Willis, Hoffman e centrifugo- flutua??o. As amostras de solo e fezes foram processadas e analisadas no laborat?rio de Cocc?dios e Coccidioses do Projeto sanidade Animal (EMBRAPA/UFRRJ). O teste do ?2 com 5% de signific?ncia foi realizado com aux?lio do programa Epinfo 2002 para avaliar a associa??o entre as preval?ncias de parasitos e as t?cnicas de diagn?stico empregadas. Ovos de Ancylostomat?deos, Toxocar?deos, Trichuris spp., Ascaris spp. e larvas de Ancylostomat?deos e Strongyloides spp. foram diagnosticados no solo de sete (28,00%) pra?as. A t?cnica de Adapta??o do m?todo de Rugai (CARVALHO et al., 2005), para an?lise do solo, foi mais eficiente que a t?cnica de Dunsmore et al. (1984) visto que ? mais barata e de f?cil execu??o al?m de detectar ovos de Ancylostomat?deos (4,80%), Ascaris spp.(1,60%), Trichuris spp. (2,40%), larvas de Ancylostomat?deos (8,80%) e de Strongyloides spp. (1,60%) e nemat?ides de vida livre (36,80%) formas que n?o foram detectadas pela t?cnica de Dunsmore et al. (1984). A preval?ncia de ovos de Toxocar?deos foi similar (p= 0,213) nas duas t?cnicas. Em 92,50% das 81 amostras fecais foi observado pelo menos um tipo de parasito em uma das t?cnicas. Os Ancylostom?deos foram os mais prevalecentes (80,25%) nas fezes dos animais, seguidos por Toxocar?deos (11,11%) cest?ides (8,64%), Cryptosporidium spp. (7,41%) e Trichuris spp. (6,17%). Em 34,57% das amostras fecais foram observadas infec??es m?ltiplas por dois e tr?s g?neros de parasitos. A baixa preval?ncia de ovos de Ancylostomat?deos nas amostras de solo das pra?as contrasta com a alta preval?ncia observada nas fezes, sugerindo que as condi??es ambientais do local s?o inadequadas ao desenvolvimento e sobreviv?ncia de suas formas infectantes, embora possam estar presentes em quantidades inferiores ?quelas capazes de serem detectadas pelas t?cnicas utilizadas. J?, a baixa preval?ncia de Toxocar?deos nas fezes quando comparada ? preval?ncia de Ancylostomat?deos indica que os animais que t?m acesso a essas pra?as s?o adultos e n?o filhotes, principais disseminadores de ovos de Toxocar?deos. Embora identificados nas fezes, nenhum cisto ou oocisto de protozo?rio foi diagnosticado nas amostras de solo, indicando a necessidade do desenvolvimento de t?cnicas diagn?stico eficazes na detec??o desses.

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