This thesis focuses on the role and historical development of strategies of experimental domestication in scientific knowledge production, with a particular focus on the function of the laboratory strains known as 'wild types' in the model organism systems of classical genetics, where they play the role of standing in for the 'natural' instance of the species so that variation may be measured. As part of establishing how lab wild types came to assume this role, I have situated them within a much longer historical trajectory that tracks how changes in the manner that European intellectual traditions conceptualised the domestic-wild divide were linked to the development of new forms of scientific domestication and knowledge production. These new developments required that existing domesticating practices be intensified, expanded and analogised in order to better control, capture and comprehend 'wild' nature. My first two chapters introduce the domestic-wild divide by discussing both contemporary and ancient interpretations of it. In my third and fourth chapter, I explore the roots of the knowledge regime of European scientific domestication. I highlight Francis Bacon's campaign to use knowledge of domesticating practices to restore human dominion, before showing how Linnaeus later re-conceptualised the natural economy as an autonomous order and original order, with domestication reinterpreted as an artful transformation of nature requiring human maintenance to prevent reversion to its wild 'natural state'. I identify this idea of the wild as original and the domestic as derivative and artificially maintained as the basis of the original wild type concept. In my fifth chapter, I discuss Darwin's attempt to unite the domestic and wild under common laws of variation and selection, including his argument that reversion was simply a product of a return to ancestral conditions of existence. I observe that Darwin's theory of variation was problematic for the effort to bring wild nature under controlled conditions for study, so in my sixth and seventh chapters discuss how this difficulty was resolved, first by experimental naturalists both before and after Darwin who utilised vivaria and microscopes to bring pieces of nature indoors, and then by Weismann and Galton's sequestration of heredity, which helped persuade scientists that domestication was not in itself a cause of germinal variation. In my eighth and ninth chapter, I detail how sequestration led the early Mendelians de Vries and Bateson to assume that wild types could be brought into the lab from nature and purified into true-breeding strains. I discuss their differing atomist and interactionist perspectives on wild type, with de Vries favouring 'elementary species' as units of nature, whereas Bateson held wild types and mutants to represent normal and abnormal forms of the species respectively. In my last chapter, I cover the replacement of Bateson's interactionist genetics by the reductionist genetics of the Morgan group and argue that this led to a disintegration of wild types into their component genes. I conclude with a discussion of what wild type strains in classical genetics were meant to be representative of, and end by establishing that whilst these strains may not wholly be representative of their species, they are nonetheless useful tools for scientific knowledge production.

Magister Legum - LLM / On 17 July 1998, a total of 120 States, including Malawi, voted for the adoption of the Rome Statute of the International Criminal Court. The ermanent ICC became operational on 1 July 2002. The ICC has jurisdiction over the crime of genocide, crimes against humanity, and war crimes. These crimes are the most serious crimes of international concern. The ICC operates under the principle of complementarity, which entails that the ICC will only assume jurisdiction over these core crimes in the event that a State Party is unwilling and unable genuinely to carry out the investigation and prosecution. States Parties have, therefore, the primary responsibility to investigate and prosecute these crimes. The States Parties must therefore establish jurisdiction to conduct investigations and prosecution of these core crimes. It is from that background, coupled with the historical evolution and development of international criminal law, with regard to individual criminal responsibility, that this paper argues for the implementation of the Rome Statute in Malawi, through domestic legislation.The paper thus argues that only through domestic legislation can the purports of the Rome Statute be achieved and fulfilled by Malawi. / South Africa

Ongoing modification and change is core to how domestic and built environments function. Thus occupants domestication and development of home heating practices around low-carbon technologies is likely to exceed what building engineering sciences have the ability to plan ahead for. Yet, environmental policies and low -carbon industry approaches to sustainable energy consumption are characterised by a high degree of technological determinism. Disciplinary approaches to sustainable energy consumption tend to separate home heating into stable, routine interaction with control points, environmental factors and socio-demographic drivers. Framing low-carbon technical change in isolation from domestic environments often leads to a gap between intended and actual use of technologies. By focusing on TRVs (thermostatic radiators valve) and radiators, this thesis takes an interdisciplinary turn to jointly examine the social and environmental elements of households energy use. A turn to sensory ethnography and practice-place relationships offers a way to better understand how people use energy for space heating in relation to the buildings they live in and how improvisatory uses of technologies emerge from flows of material, domestic, sensory and physical contingencies of the home. Combining home video tours with building energy monitoring in eight homes, the thesis demonstrates that home heating is a place-event of the home because heating systems and energy consumption are woven into the fabric of everyday life. Environmental elements show that the social and technical are inseparable in energy used for space heating and individual elements imply that the domestication of technologies is highly unpredictable. The thesis synthesises findings into a taxonomy table of irregular radiator and TRV use. On the one hand, irregularities indicate that improvisatory uses of technologies are productive sources of sustainable change because they can be potential sites for co-design. On the other hand, the interwoven character of the social and technical in households energy use critically challenges how environmental policy, low-carbon industry and disciplinary approaches frame intervention into sustainable energy consumption. The thesis argues for the value of logic of intervention and sustainable change that is collaborative, system-focused and gradually uncovers interrelationships.

Os elementos de transposição (TEs) se movimentam de um locus para outro no genoma afetando a estrutura e evolução destes. A superfamília de transposases hAT é definida pelos elementos que compartilham os domínios de dimerização e ligação ao DNA com os transposons previamente descritos: hobo, Activator e Tam3. Análises prévias encontraram algumas evidencias da presença genômica e ativação transcricional de TEs relacionados à superfamília hAT em cana-de-açúcar (denominados de família SChAT) e pelo menos três linhagens evolutivas foram postuladas. O objetivo deste trabalho é caracterizar versões genômicas das linhagens de transposons (191 e 257) e linhagem possivelmente domesticada (074). Pretende-se estudar as relações evolutivas, distribuição em gramíneas, identificar os padrões de expressão e propriedades funcionais. Regiões de sintenia foram estabelecidas para estes BACs em Arabidopsis thaliana, Brachypodium distachyon, Sorghum bicolor, Oryza sativa e Zea mays. Elementos relacionados com as três linhagens foram procurados nestes genomas. / Transposable elements (TEs) are able to move from one locus to another within a genome. TE mobilization affects genome structure and evolution. The hAT transposase superfamily is defined as elements that share the dimerization and DNA ligation domains with the previously described hobo, Activator and Tam3 transposon elements. Previous analyses found some genomic and transcriptional evidences of TEs related to hAT superfamily in sugarcane (named SChAT family) and at least three evolutionary lineages were proposed. The aim of this work is to characterize full-length genomic versions of the transposons lineages (191 and 257) and from the domesticated lineage (074). It is proposed to study the evolutionary relationship, distribution along grasses genomes, identify expression patterns and functional capacities of the SChAT elements. Syntenic regions for the BACs containing elements from the three lineages were mapped in Arabidopsis thaliana, Brachypodium distachyon, Sorghum bicolor, Oryza sativa and Zea mays. Related elements were search on the same genomes.

Domestic fowl (Gallus gallus f. domestica) is an economically important model species in science. Knowledge of the immune system of this species is therefore crucial. In research the commercial stocks are usually used together with laboratory inbred lines. They have an unnatural variability, as a result of inbreeding, their genetic and phenotypic variability is reduced. This laboratory lines are therefore not the best model for research of variability. Presently, genetically diversified poultry populations can be found in rural stocks and fancy breeds. These breeds could be good model for research in variability of immunoresponse. In this thesis, I try to map the genetic variability of the binding site of MHC class I glycoproteins in fowl breeds. In chickens are these molecules encoded in duplicated gene called BF (BF1 and BF2). These genes are relatively well known and considerable variability has been described in domestic fowl. Our results suggest a high genetic variability of MHC I in domestic fowl breeds. We had identified 41 haplotypes in 25 individuals of 14 breeds, including 7 haplotypes already known from the literature. I also focused on proinflammatory activity in fowl breeds. I watched swelling of tissue and expression of pro-inflammatory cytokines IL-6 and IL-1 after subcutaneous...

Cultural adaptation and free translation strategies are more common to use within the field of video game localization although they are still not recommended too veruse in translation studies. Free translation strategies are commonly used invideo game localization to make video games ready for a targeted audience. As free translation is mostly target-oriented in video game localization, the original source text often loses its meaning because of culturally adapting and modifying the text to fit to the target culture. This study aims to analyze 10 chosen cutscenes of the video game Lost Judgement, to find how frequently cultural adaptation and free translation strategies were used for pragmatic (proper names, cultural termsand expressions, allusion) and interlingual (regional dialect and slang) translation problems and how these problems were solved. The result and analysis of the study demonstrate examples of these translation problems within chosen cutscenes. The examples prove that depending on translation problems, both free translation and cultural adaptation can be used in combination with a literal word-for-word translation strategy. It also indicates that the video localization of Lost Judgement implied to use both domestication and foreignization approaches to achieve a higher satisfaction for the players of the game who can choose their favorite version. Another important factor was that the localization of the videogame focused on a functional approach whose purpose was to keep loyalty to the original text, the source text.

Tillers are vegetative branches found in grasses, which develop in early stages of plant life. Located at the base of the central stalk, tillers have agronomical importance by increasing seed production with fewer tillers, or providing alternative forms of biofuel with more tillers. As grains have typically decreased tiller number while undergoing domestication, we explored wild and domesticated strains of varying grains by doing a morphological analysis on tiller development. This thesis shows how the decrease of tillers through in domestication cereals shows diversity not only across maize, Sorghum, and Setaria, but also between lines of maize and Setaria species. To do so, we first measured axillary bud growth across these grasses and compared bud initiation, growth, dormancy and outgrowth. While maize inbred B73 demonstrated a tiller dormancy pattern by initiating buds, growing buds and then bud dormancy we measured growth in Sorghum and Setaria to compare and found that although Sorghum patterns dormancy similar to maize, Setaria had more than one way tiller suppression not previously expected. We look further at Setaria buds with a statistical analysis of tiller origin and bud frequency in a wild strain and two domesticated strains of Setaria. Furthermore we performed Scanning Electron Microscopy (SEM) to have a clear understanding of bud initiation or lack of initiation in Setaria italica (B100) comparing it to its wild ancestor Setaria viridis. Because of the diversity in Setaria, we re-visited maize tiller domestication by taking bud measurements, performing SEMs and counting bud frequency on other strains of inbred maize. We found that maize also shows diversity in its patterning of tiller domestication. These results demonstrate that there is diversity in the patterns in which tiller domestication has occurred. This diversity is shown here through differences in tiller bud decisions to initiate or not initiate, or to have axillary buds go dormant post-initiation. Furthermore this variance is shown through differences in bud frequency counts, growth measurements, SEMs, and where tiller branches originate across the grains of maize, Sorghum and Setaria.

The emergence of open educational resources has gained popularity and acceptance in higher education institutions and beyond the basic education sector. This brought a persisting shift in depending on information communication technologies for tuition and research provision. Information technology artifact was not treated in isolation to user perspective. The study established how academics accept, feel, perceive, and what skills, opportunities, challenges exist to hinder the domestication. The study context had no uniform guidelines or tools and policy in place for the domestication of open educational resources. The study adopted the exploratory approach guided by the interpretivism paradigm. The study employed Domestication theory. This study conducted in an heterogenous single case study, which is the open distance e-learning (University of South Africa). That was done for an in-depth investigation by relying on multi-methods for data triangulation such as semi-structured interviews, focus group interviews, document analysis, and actual artifact analysis. The total of participants were 52. The study found that most academics played a role in the domestication of open educational resources besides the minority who were unable. The experience and prior knowledge were found to be a factor hindering the domestication process. Open distance e-learning found to relevant space for open educational resources. Such institutions play a role in the adoption and development of open educational resources and mostly rely in information technology for tuition and research. Information technology infrastructure found to be an enabler and disabler in the domestication process. This study contribution to the world of knowledge is based on the theory and practice. Eight theoretical propositions were suggested. The study further contributed by extension of domestication theory as recommended two additional phases which are non-appropriation and dis-appropriation. The current proposed Domestication theory has five phases. Lastly, the study recommended the actual guidelines for adoption and development of open educational resources. This guideline can be adopted by higher education institutions by infusing them in policy development or for general guidance in actual adoption and developments.

This study presents mitochondrial genome sequences from 22 Egyptian house cats with the aim of resolving the uncertain origin of the contemporary world-wide population of Domestic cats. Together with data from earlier studies it has been possible to confirm some of the previously suggested haplotype identifications and phylogeny of the Domestic cat lineage. Moreover, by applying a molecular clock, it is proposed that the Domestic cat lineage has experienced several expansions representing domestication and/or breeding in pre-historical and historical times, seemingly in concordance with theories of a domestication origin in the Neolithic Middle East and in Pharaonic Egypt. In addition, the present study also demonstrates the possibility of retrieving long polynucleotide sequences from hair shafts and a time-efficient way to amplify a complete feline mitochondrial genome.

Denna studie undersöker småbarnsföräldrars syn på mediefostran i ett alltmer digitaliserat medielandskap. Ur perspektiv om domesticering, mediepraktiker och uppfostran utforskar studien hur föräldrar hanterar sina barns interaktion med medier i vardagen. Genom medieetnografisk metod och kvalitativa semistrukturerade intervjuer med småbarnsföräldrar framträder en bild av hur familjer balanserar mellan acceptans och motstånd till olika medier, med betoning på hur medieanvändningen integreras i familjens vardagliga rutiner och sociala praktiker. Resultaten synliggör en komplex väv av familjedynamik, samhällsnormer och teknologisk utveckling, som tillsammans påverkar hur medier domesticeras i hushållen. Studien bidrar på så vis nya insikter om hur små barns medieanvändning blir en del av småbarnfamiljers vardag. / This study examines the views of parents of young children on media parenting in an increasingly digital media landscape. From the perspectives of domestication theory, media practices, and upbringing, the study explores how parents manage their children's interaction with media in everyday life. The study investigates how parents' attitudes towards media shape children's media habits. Through media ethnographic methods and qualitative semi-structured interviews with parents of young children, a picture emerges of how families balance between acceptance and resistance to different types of media, emphasizing how media use is integrated into the family's daily routines and social practices. The studys results show a complex web of family dynamics, moral economy, and technological development, which together influence how media are domesticated in households. Thus, the study contributes with new insights regarding how young childrens media use is a part of the everyday life of families.