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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Showing 311 to 320 of 683 (0.065 seconds)| 311 |
Metoder och tillämpningar av CRISPR-Cas9 i cancerforskning. : Samt hur CRISPR-Cas9 kan implementeras i skolundervisningen. / Methods and applications of CRISPR-Cas9 in cancer research. : – And how CRISPR-Cas9 can be applied in teaching.Valladares, Rodrigo, Briheim, Hanna January 2020 (has links)
CRISPR-Cas9 är ett effektivt genredigeringsverktyg som har upptäckts på senare år. Verktyget härstammar från ett adaptivt immunförsvar hos prokaryoter. Tekniken används för att modifiera DNA hos växter, djur och människor på ett enkelt och billigt sätt. CRISPR-Cas9 har visat sig ha stor potential vid bekämpning av olika sjukdomar däribland cancer som idag är ett globalt hälsoproblem. Inom cancerforskningen ses CRISPR-Cas9 som ett lovande verktyg vid cancerterapi och läkemedelsutveckling. I denna studie sammanställer vi aktuella metoder och användningsområden med CRISPR-Cas9 inom cancerforskning. Dessutom undersöker vi hur denna form av genteknik kan lyftas upp och tillämpas i biologiundervisningen. / CRISPR-Cas9 has recently emerged as an effective genome editing tool. The tool derives from an adaptive immune system in prokaryotes. The technology is used for modification of DNA in plants, animals and humans in a simple and inexpensive way. CRISPR-Cas9 has shown great potential in fighting different diseases like cancer which today is a global health issue. It is seen as a promising tool for cancer research when it comes to cancer therapy and drug development. Here we summarize current methods and applications of CRISPR-Cas9 for cancer research. Furthermore, we explore the possibilities of introducing and applying this kind of genetic engineering in biology teaching. / <p>Framläggning, opponering och respondering skedde skriftligt till följd av covid19.</p>
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Phenotype-Based High-Throughput Classification of Long QT Syndrome Subtypes Using Human Induced Pluripotent Stem Cells / ヒト人工多能性幹細胞を利用した、QT延長症候群の表現型に基づくハイスループット判別法Yoshinaga, Daisuke 23 March 2020 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第22335号 / 医博第4576号 / 新制||医||1041(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 山下 潤, 教授 岩田 想, 教授 木村 剛 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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Generation of macrophages with altered viral sensitivity from genome-edited rhesus macaque iPSCs to model human disease / 非ヒト霊長類疾病モデル作成を目的としたゲノム編集アカゲザルiPSCからのウイルス感受性を変化させたマクロファージの再生Iwamoto, Yoshihiro 26 July 2021 (has links)
京都大学 / 新制・課程博士 / 博士(医学) / 甲第23413号 / 医博第4758号 / 新制||医||1052(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 竹内 理, 教授 小柳 義夫, 教授 濵﨑 洋子 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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Activation of endogenous full-length active LINE-1 RNA using CRISPR activation to study its role during somatic cell reprogrammingAlsolami, Amjad 11 1900 (has links)
The repetitive sequence composes nearly half of human and mouse genome, most of which are scattered repeats of transposable elements (TEs). The non-LTR retrotransposons are the most accumulated TEs in the mammalian genome and L1s are the most active and abundant autonomous retrotransposons. L1s are highly activated during the epigenetic reprogramming of early mammalian embryos and have the highest level of expression among all retrotransposons throughout the preimplantation state. Moreover, the reprogramming of somatic cells into iPSCs is associated with an increase in L1 expression. The transcription of L1 during the early embryogenesis is necessary to regulate developmental genes and prevent heterochromatin formation to maintain cellular pluripotency state, that guarantying an appropriate future differentiation. However, the role of L1 reactivation during the somatic cell reprogramming remains unclear. Therefore, aim of this work is to study the impact of L1 transcription during the reprogramming process of the iPSCs. We used CRISPR-mediated gene activation (CRISPRa) system that fuse a deactivated Cas9 (dCas9) with transactivation domains (VPR). We confirm the ability to overexpress L1 in Human Embryonic Kidney cells (HEK293) and Human Dermal Fibroblasts (HDFs) by utilizing CRISPR activation system and this will provide a good opportunity to study the role of L1 transcripts during the reprogramming of HDFs into iPSCs. Furthermore, we established stable HDFs that able to express combinations of “Yamanaka” reprogramming factors. The model system will allow to investigate the effect of overexpressing L1 with reprogramming factors to answer the question of whether L1 can trigger or facilitate the reprogramming processes and its underlying mechanism.
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Dokumentace brzdných stop pomocí fotoaparátu / Documentation of Braking Traces Using a CameraKopecký, Štěpán January 2018 (has links)
The diploma thesis describes the measurement and evaluation of braking traces in cars equipped by system ABS. The result of the work should contribute to improving the quality of photodocumentation of braking traces. To creating a procedure to eliminate as much as possible the loss of information during tracking documentation.
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Collaborative Text Editing in a Portal / Collaborative Text Editing in a PortalKorčák, Ján January 2012 (has links)
V tomto texte sa zameriame na populárnu koncepciu kolaboratívnej tvorby dokumentov. Predstavíme si myšlienku využitia tohto mechanizmu v rôznych oblastiach rozhodovania, popíšeme si koncept a princíp fungovania. Následne si predstavíme a rozoberieme portály a portletovú technológiu, ich výhody a využitie. Cieľom práce je implementácia kolaboratívneho editora s využitím knižnice pre prácu so zmenami v dokumentoch s perzistentnou a aplikačnou logikou na platforme JEE a vytvorenie jednoduchého portletu pre túto službu.
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Eine Greedy-Heuristik für die Lösung des Cograph Editing ProblemsHainke, Kai-Adrian 19 February 2018 (has links)
Diese Arbeit betrachtet das Cograph Editing Problem im Kontext der Rekonstruktion phylogenetischer Bäume. Insbesondere muss dafür ein Cograph rekonstruiert werden, der durch Rauschen verzerrt wurde. Liu et al. zeigten, dass Cograph Editing NP-vollständig ist. Damit ist das Problem für große Graphen wahrscheinlich nicht in realistischer Zeit korrekt lösbar. Diese Arbeit stellt daher eine Greedy-Heuristik für die Lösung des Cograph Editing Problems vor. Dabei werden Editieroperationen als günstig betrachtet, wenn sie die Anzahl an induzierten P4 in dem Graphen minimieren. Es wird ein on-line Algorithmus zur Berechnung dieser Heuristik vorgestellt und analysiert. Anschließend wird ein Greedy-Algorithmus formuliert, der eine heuristische Lösung des Cograph Editing Problems in O(|V|^4) Zeit und unter Nutzung von O(|V|^2) Speicher berechnet. Eine Implementierung dieses Greedy-Algorithmus wird auf zufällig generierten Testdaten untersucht. Parallel dazu wird auch eine Implementierung für die korrekte Lösung des Cograph Editing Problems getestet. Dabei werden die Qualität der Ergebnisse und die Laufzeit gemessen und verglichen. Hier zeigte sich, dass der Greedy-Algorithmus bei der Rekonstruktion von durch Rauschen verzerrter Cographen vergleichbar gute Ergebnisse erzielen konnte.
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Post-Editing als Bestandteil von Translationsstudiengängen in der DACH-Region: Ergebnisse einer Online-BefragungSchumann, Paula 25 May 2020 (has links)
No description available.
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Architectures and Algorithms for Real-Time Web-Based CollaborationGadea, Cristian 31 March 2021 (has links)
Originating in the theory of distributed computing, the optimistic consistency control method known as Operational Transformation (OT) has been studied by researchers since the late 1980s. Algorithms were devised for managing the concurrent nature of user actions and for maintaining the consistency of replicated data as changes are introduced by multiple geographically-distributed users in real-time. Web-Based Collaborative Platforms are now essential components of modern organizations, with real-time protocols and standards such as WebSocket enabling the development of online collaboration tools to facilitate information sharing, content creation, document management, audio and video streaming, and communication among team members. Products such as Google Docs have shown that centralized web-based co-editing is now possible in a reliable way, with benefits in user productivity and efficiency. However, as the demand for effective real-time collaboration between team members continues to increase, web applications require new synchronization algorithms and architectures to resolve the editing conflicts that may appear when multiple individuals are modifying the same data at the same time. In addition, collaborative applications need to be supported by scalable distributed backend services, as can be achieved with "serverless" technologies. While much existing research has addressed problems of optimistic consistency maintenance, previous approaches have not focused on capturing the dynamic client-server interactions of OT systems by modeling them as real-time systems using Finite State Machine (FSM) theory. This thesis includes an exploration of how the principles of control theory and hierarchical FSMs can be applied to model the distributed system behavior when processing and transforming HTML DOM changes initiated by multiple concurrent users. The FSM-based OT implementation is simulated, including with random inputs, and the approach is shown to be invaluable for organizing the algorithms required for synchronizing complex data structures. The real-time feedback control mechanism is used to develop a Web-Based Collaborative Platform based on a new OT integration algorithm and architecture that brings "Virtual DOM" concepts together with state-of-the-art OT principles to enable the next generation of collaborative web-based experiences, as shown with implementations of a rich-text editor and a 3D virtual environment.
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The CRISPR-Cas systemStens, Cassandra, Enoksson, Isabella, Berggren, Sara January 2020 (has links)
Derived from and inspired by the adaptive immune system of bacteria, CRISPR has gone from basic biology knowledge to a revolutionizing biotechnological tool, applicable in many research areas such as medicine, industry and agriculture. The full mechanism of CRISPR-Cas9 was first published in 2012 and various CRISPR-Cas systems have already passed the first stages of clinical trials as new gene therapies. The immense research has resulted in continuously growing knowledge of CRISPR systems and the technique seems to have the potential to greatly impact all life on our planet. Therefore, this literature study aims to thoroughly describe the CRISPR-Cas system, and further suggest an undergraduate laboratory exercise involving gene editing with the CRISPR-Cas9 tool. In this paper, we describe the fundamental technical background of the CRISPR-Cas system, especially emphasizing the most studied CRISPR-Cas9 system, its development and applications areas, as well as highlighting its current limitations and ethical concerns. The history of genetic engineering and the discovery of the CRISPR system is also described, along with a comparison with other established gene editing techniques. This study concludes that a deeper knowledge about CRISPR is important and required since the technique is applicable in many research areas. A laboratory exercise will not only inspire but also provide extended theoretical and practical knowledge for undergraduate students.
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