Made available in DSpace on 2014-12-17T15:01:34Z (GMT). No. of bitstreams: 1 PedroFSF_DISSERT.pdf: 2707475 bytes, checksum: 2a6ef9226a3d6d607615f74305bb9e07 (MD5) Previous issue date: 2014-05-09 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The need for new sources of energy and the concern about the environment have pushed the search for renewable energy sources such as ethanol. The use of lignocellulosic biomass as substrate appears as an important alternative because of the abundance of this raw material and for it does not compete with food production. However, the process still meets difficulties of implementation, including the cost for production of enzymes that degrade cellulose to fermentable sugars. The aim of this study was to evaluate the behavior of the species of cactus pear Opuntia ficus indica and Nopalea cochenillifera, commonly found in northeastern Brazil, as raw materials for the production of: 1) cellulosic ethanol by simultaneous saccharification and fermentation (SSF) process, using two different strains of Saccharomyces cerevisiae (PE-2 and LNF CA-11), and 2) cellulolytic enzymes by semi-solid state fermentation (SSSF) using the filamentous fungus Penicillium chrysogenum. Before alcoholic fermentation process, the material was conditioned and pretreated by three different strategies: alkaline hydrogen peroxide, alkaline using NaOH and acid using H2SO4 followed by alkaline delignification with NaOH. Analysis of composition, crystallinity and enzymatic digestibility were carried out with the material before and after pretreatment. In addition, scanning electron microscopy images were used to compare qualitatively the material and observe the effects of pretreatments. An experimental design 2? with triplicate at the central point was used to evaluate the influence of temperature (30, 40 and 45 ?C) and the initial charge of substrate (3, 4 and 5% cellulose) in the SSF process using the material obtained through the best condition and testing both strains of S. cerevisiae, one of them flocculent (LNF CA-11). For cellulase production, the filamentous fungus P. chrysogenum was tested with N. cochenillifera in the raw condition (without pretreatment) and pretrated hydrothermically, varying the pH of the fermentative medium (3, 5 and 7). The characterization of cactus pear resulted in 31.55% cellulose, 17.12% hemicellulose and 10.25% lignin for N. cochenillifera and 34.86% cellulose, 19.97% hemicellulose and 15.72% lignin for O. ficus indica. It has also been determined, to N. cochenillifera and O. ficus indica, the content of pectin (5.44% and 5.55% of calcium pectate, respectively), extractives (26.90% and 9.69%, respectively) and ashes (5.40% and 5.95%). Pretreatment using alkaline hydrogen peroxide resulted in the best cellulose recovery results (86.16% for N. cochenillifera and 93.59% for O. ficus indica) and delignification (48.79% and 23.84% for N. cochenillifera and O. ficus indica, respectively). This pretreatment was also the only one which did not increase the crystallinity index of the samples, in the case of O. ficus indica. However, when analyzing the enzymatic digestibility of cellulose, alkali pretreatment was the one which showed the best yields and therefore it was chosen for the tests in SSF. The experiments showed higher yield of conversion of cellulose to ethanol by PE-2 strain using the pretreated N. cochenillifera (93.81%) at 40 ?C using 4% initial charge of cellulose. N. cochenillifera gave better yields than O. ficus indica and PE-2 strain showed better performance than CA-11. N. cochenillifera proved to be a substrate that can be used in the SSSF for enzymes production, reaching values of 1.00 U/g of CMCase and 0.85 FPU/g. The pretreatment was not effective to increase the enzymatic activity values / A necessidade de novas fontes de energia e a preocupa??o com o meio-ambiente t?m impulsionado a pesquisa por fontes renov?veis de energia, como o etanol. O uso de biomassa lignocelul?sica como substrato aparece como uma importante alternativa devido ? abund?ncia desta mat?ria-prima e por n?o concorrer com a produ??o de alimentos. Entretanto, o processo ainda encontra dificuldades de implementa??o, entre elas o custo para produ??o das enzimas que degradam a celulose em a??cares fermentesc?veis. O objetivo deste trabalho foi avaliar o comportamento das esp?cies de palma forrageira Opuntia ficus indica (gigante) e Nopalea cochenillifera (mi?da), comumente encontradas na regi?o Nordeste do Brasil, como mat?rias-primas para produ??o de: 1) etanol celul?sico pelo processo de sacarifica??o e fermenta??o simult?neas (SFS) usando duas cepas diferentes de Saccharomyces cerevisiae (PE-2 e LNF CA-11) e 2) enzimas celulol?ticas atrav?s da fermenta??o em estado semiss?lido (FES) usando o fungo filamentoso Penicillium chrysogenum. Antes do processo de fermenta??o alco?lica, o material foi condicionado e pr?-tratado por tr?s diferentes estrat?gias: per?xido de hidrog?nio alcalino, alcalino usando NaOH e ?cido usando H2SO4 seguido de deslignifica??o alcalina com NaOH. An?lises de composi??o, cristalinidade e digestibilidade enzim?tica foram feitas com o material antes e depois do pr?-tratamento. Adicionalmente, imagens de microscopia eletr?nica de varredura foram usadas para comparar qualitativamente o material e observar os efeitos dos pr?-tratamentos. Um planejamento fatorial 2? com triplicata no ponto central foi utilizado para avaliar a influ?ncia da temperatura (30, 40 e 45 ?C) e da carga inicial de substrato (3, 4 e 5% de celulose) no processo SFS, usando o material obtido nas melhores condi??es de pr?-tratamento e testando duas cepas de S. cerevisiae, sendo uma delas floculante (LNF CA-11). Para a produ??o de celulase, o fungo filamentoso P. chrysogenum foi testado com a esp?cie de palma N. cochenillifera no estado in-natura (sem pr?-tratamento) e submetida a um pr?-tratamento hidrot?rmico, variando-se o pH do meio fermentativo (3, 5 e 7). A caracteriza??o das palmas forrageiras resultou em 31,55% de celulose, 17,12% de hemicelulose e 10,25% de lignina para a esp?cie N. cochenillifera e 34,86% de celulose, 19,97% de hemicelulose e 15,72% de lignina para a esp?cie O. ficus indica. Analisou-se ainda, para as palmas mi?da e gigante, o teor de pectina (5,44% e 5,55% de pectato de c?lcio, respectivamente), extrativos (26,90% e 9,69%, respectivamente) e cinzas (5,40% e 5,95%). O pr?-tratamento usando per?xido de hidrog?nio alcalino apresentou os melhores resultados de recupera??o de celulose (86,16% para a palma mi?da e 93,59% para a palma gigante) e de deslignifica??o (48,79% e 23,84% para as palmas mi?da e gigante, respectivamente). Este pr?-tratamento foi tamb?m o ?nico a n?o elevar o ?ndice de cristalinidade das amostras, no caso da palma gigante. Entretanto, quando analisada a digestibilidade enzim?tica da celulose, o pr?-tratamento alcalino foi o que proporcionou os melhores rendimentos e, portanto, este foi o escolhido para os testes de SFS. Os experimentos demonstraram maior rendimento da convers?o de celulose em etanol pela cepa PE-2 usando a palma mi?da pr?-tratada (93,81%) a 40 ?C e usando 4% de carga inicial de celulose. A palma mi?da demonstrou melhores rendimentos que a gigante e a cepa PE-2 resultou melhor desempenho que a CA-11. A palma mi?da se mostrou um substrato poss?vel de ser usado na FES para produ??o de enzimas, alcan?ando valores de 1,00 U/g de CMCase e 0,85 FPU/g. O pr?-tratamento n?o se mostrou eficaz para aumentar os valores de atividade enzim?tica

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-01-26T16:31:14Z No. of bitstreams: 1 2016 - Danilo Antonio Morenz.pdf: 881703 bytes, checksum: 6cfcd4c0f7c8a5dd52386788991fd786 (MD5) / Made available in DSpace on 2018-01-26T16:31:14Z (GMT). No. of bitstreams: 1 2016 - Danilo Antonio Morenz.pdf: 881703 bytes, checksum: 6cfcd4c0f7c8a5dd52386788991fd786 (MD5) Previous issue date: 2016-05-31 / CAPES / The aim was evaluate the effect of replacing corn by bakery waste (BW) in goats diet on performance, dry matter and nutrients intake, carcass characteristics, chemical composition and fatty acid profile of the Longissimus dorsi muscle and the food intake, apparent digestibility, nitrogen balance, ruminal parameters, production and composition of milk by goats. Experiment 1: Four levels of inclusion of BW replacing corn (0, 33, 66, 100%) in the diet of kids distributed in a completely randomized design were analyzed. The diets were composed of concentrate and Cynodon spp. hay, with forage: concentrate ratio of 60:40. The intakes of dry matter, organic matter, crude protein, ether extract and carbohydrates decreased linearly, while the non-fiber carbohydrates intake, animal performance, carcass characteristics and chemical composition were not affected by replacement of corn by BW. The elaidic acid content (C18: 1 trans-9) increased in the fatty acid profile of the Longissimus dorsi muscle with the inclusion of RP, which can be used as a substitute for corn meal in goats feed composition. It was concluded that bakery waste can substitute the corn up to 100% in the concentrate mixture without affecting intake, performance and carcass and meat traits of kids. Experiment 2: Bakery waste was added replacing 0; 25; 50; 75 and 100% of the concentrate in dry matter basis. Five Saanen lactating goats, non-pregnant with an average weight of 55.7 kg were arranged in a 5 x 5 Latin Square design. Experimental diets were composed by Cynodon hay and concentrate, in a roughage:concentrate ration of 40:60. The inclusion of BW in diets decreases the intake of ether extract, acid detergent fiber and the coefficients of apparent digestibility of crude protein and ether extract. Different levels of substitution did not affect ruminal pH, but for the concentration of ruminal ammonia was found linear reduction with the level of replacement. The inclusion of BW in diets increased the efficiency of use of N. The bakery waste can replace conventional concentrate in diets for goats. / Objetivou-se avaliar o efeito da substitui??o do milho pelo res?duo de panifica??o (RP) na dieta de cabritos sobre o desempenho, consumo de mat?ria seca e dos nutrientes, caracter?sticas de carca?a, composi??o qu?mica e perfil dos ?cidos graxos do m?sculo Longissimus dorsi e em cabras em lacta??o o consumo alimentar, a digestibilidade aparente, o balan?o de compostos nitrogenados, os par?metros ruminais, a produ??o e a composi??o do leite. Experimento 1: Foram analisados quatro n?veis de inclus?o do RP em substitui??o ao milho (0, 33, 66, 100%) na dieta de cabritos distribu?dos em delineamento inteiramente casualizado. As dietas foram compostas de concentrado e feno de Cynodon spp, com rela??o volumoso:concentrado de 60:40. O consumo de mat?ria seca (MS), mat?ria org?nica (MO), prote?na bruta (PB), extrato et?reo (EE) e carboidratos totais (CT) foi linear decrescente, enquanto que o consumo de carboidratos n?o fibrosos (CNF), o desempenho dos animais, as caracter?sticas de carca?a e a composi??o centesimal n?o foram influenciados pela inclus?o do RP em substitui??o ao milho. O teor de ?cido ela?dico (C 18:1 trans-9) aumentou no perfil de ?cidos graxos do m?sculo Longissimus dorsi com a inclus?o do RP. Conclui-se que o RP pode substituir o milho em at? 100% na mistura do concentrado. Experimento 2: Foram avaliados cinco n?veis de substitui??o do concentrado pelo RP (0, 25, 50, 75, 100%), utilizando-se cinco cabras em lacta??o da ra?a Saanen, com peso m?dio de 55,7 kg, distribu?dos em delineamento em Quadrado Latino 5 X 5. As dietas experimentais foram compostas de concentrado e feno de Cynodon spp, numa rela??o volumoso:concentrado de 40:60 na mat?ria seca. Houve redu??o linear do consumo de EE, do consumo de FDA, da digestibilidade da PB e da digestibilidade do EE. A substitui??o crescente do concentrado pelo RP na dieta n?o influenciou o pH ruminal, por?m reduziu a concentra??o de N-NH3 no l?quido ruminal. A inclus?o do RP em n?veis crescentes nas dietas favoreceu a efici?ncia de utiliza??o de nitrog?nio. O RP pode substituir totalmente o concentrado na dieta de cabras em lacta??o.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-19T13:34:45Z No. of bitstreams: 5 hil.pdf: 1837336 bytes, checksum: 915b40a5d9c4387507e76471c6ae2ca7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-02-20T11:01:25Z (GMT) No. of bitstreams: 5 hil.pdf: 1837336 bytes, checksum: 915b40a5d9c4387507e76471c6ae2ca7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) / Made available in DSpace on 2015-02-20T11:01:25Z (GMT). No. of bitstreams: 5 hil.pdf: 1837336 bytes, checksum: 915b40a5d9c4387507e76471c6ae2ca7 (MD5) license_url: 52 bytes, checksum: 3d480ae6c91e310daba2020f8787d6f9 (MD5) license_text: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) license.txt: 2110 bytes, checksum: b4c884761e4c6c296ab2179d378436d4 (MD5) Previous issue date: 2013-02-20 / Funda??o de Amparo ? Pesquisa do estado de Minas Gerais (FAPEMIG) / Para a manuten??o das pr?ticas econ?micas e s?cio-culturais constru?das sobre a explora??o e transforma??o do petr?leo, faz-se necess?rio criar alternativas que n?o rompam de forma estanque com as pr?ticas industriais hoje vigentes. Nesse contexto, o uso de biomassas para a produ??o de combust?veis l?quidos que alimentem motores ? combust?o permite sustentar pr?ticas antigas com novos benef?cios. Dessa forma, a presente disserta??o teve por objetivo avaliar o potencial da torta de maca?ba, um coproduto da ind?stria de ?leos vegetais, como insumo para produ??o de bioetanol. Tal objetivo foi trabalhado atrav?s de estudos de sacarifica??o da torta de maca?ba com posterior processo fermentativo. A biomassa em quest?o foi caracterizada quimicamente, o que indicou a presen?a de aproximadamente 50% de carboidratos, distribu?dos em, 23,16 ? 0,95 % de amido, 11,49 ? 1,08% de celulose, 9,6 ? 0,79% de hemicelulose e 11,48 ? 0,62 % de a??cares sol?veis. Para a primeira interven??o da despolimeriza??o dos polissacar?deos presentes na torta de maca?ba foi realizado um planejamento experimental fatorial 25-1 com 5 fatores e 4 pontos centrais com as seguintes vari?veis: concentra??o de ?cido sulf?rico, tempo de pr?-tratamento ?cido, raz?o s?lido/l?quido, concentra??o de amiloglicosidase e concentra??o de celulase. Na sequ?ncia, o processo de sacarifica??o foi otimizado com o uso de delineamento composto central rotacional 23, com 3 fatores, 4 pontos centrais e 6 pontos axiais, onde foram avaliadas as concentra??es de ?cido sulf?rico, amiloglicosidase e celulase. Um hidrolisado obtido em condi??o otimizada com 92% de efici?ncia foi submetido ? destoxifica??o com carv?o ativado e, em seguida, foram realizados ensaios de fermentabilidade conduzidos como as leveduras Saccharomyces cerevisiae e Pichia stipitis. A levedura S. cerevisiae se mostrou resistente ao hidrolisado n?o destoxificado, apresentando YP/S de 0,49. P. stipitis mostrou-se suscept?vel ao meio n?o destoxificado, mas foi capaz de converter 99% dos a??cares redutores presentes no meio destoxificado. A torta de maca?ba apresentou potencial consider?vel para produ??o de bioetanol. A partir dos dados de bancada ? poss?vel estimar uma produ??o de 104l de etanol por tonelada de torta de maca?ba. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT For the maintenance of economic and socio-cultural practices built on the exploitation and processing of oil it is necessary to create alternatives that do not break so tight with current industrial practices. In this context, the use of biomass to produce liquid fuels that are fed into to combustion engines allows to sustain old practices with new benefits. Thus, this dissertation aimed to evaluate the potential of macaw palm coconut cake, a co-product of vegetable oil industry, as an input for bioethanol production. This goal was worked out through studies of the process of saccharification of macaw palm coconut cake with subsequent fermentation. The biomass in question was characterized chemically, what indicated the presence of approximately 50% of carbohydrates, among which, 23,16 ? 0,95% of starch, 11,49 ? 1,08% of cellulose, 9,6 ? 0,79% of hemicellulose and 11,48 ? 0,62% of soluble sugars. For the first intervention of deconstruction of the polysaccharides present in the macauba cake it was held a 25-1 factorial experimental design with five factors and four central points with the following variables: concentration of sulfuric acid, time of acid pretreatment, solid / liquid ratio, amyloglucosidase and cellulose concentrations. Following, the saccharification process was optimized using central composite rotational design 23, with three factors, four central points and six axial points, whereas the concentrations of sulfuric acid, cellulose and amyloglucosidase were evaluated. A hydrolyzed obtained in optimum condition with 92% efficiency was subjected to detoxification with activated charcoal and then, fermentability assays were carried out with yeasts Saccharomyces cerevisiae and Pichia stipitis. The yeast S. cerevisiae has proved resilient to the non-detoxified hydrolyzate and presented YP/S of 0.49. P. stipitis was found to be susceptible to the non-detoxified medium, but it was able to convert 99% of reducing sugars present in the detoxified medium. The macaw palm coconut cake showed considerable potential for bioethanol production. From the lab bench data it is possible to estimate a production of 104 l of ethanol per ton of macaw palm coconut cake.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-11-17T17:43:39Z No. of bitstreams: 2 patricia_monteiro_costa.pdf: 502036 bytes, checksum: 032cd25c3dc0c50f0fb888d41c9d7414 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2015-11-17T17:44:18Z (GMT) No. of bitstreams: 2 patricia_monteiro_costa.pdf: 502036 bytes, checksum: 032cd25c3dc0c50f0fb888d41c9d7414 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) / Made available in DSpace on 2015-11-17T17:44:18Z (GMT). No. of bitstreams: 2 patricia_monteiro_costa.pdf: 502036 bytes, checksum: 032cd25c3dc0c50f0fb888d41c9d7414 (MD5) license_rdf: 23898 bytes, checksum: e363e809996cf46ada20da1accfcd9c7 (MD5) Previous issue date: 2011-04-28 / Foram realizados dois experimentos para avalia??o das caracter?sticas produtivas e qualitativas de forragens produzidas em sistemas de cons?rcio milho, capim-braqui?ria e diferentes leguminosas. No primeiro, avaliou-se a produtividade, composi??o bromatol?gica e perfil fermentativo das silagens dos seguintes tratamentos: milho em cultivo exclusivo (MCE); cons?rcio milho, capim-braqui?ria (MB), cons?rcio milho, capim-braqui?ria e Calopogonium mucunoides (MBC); cons?rcio milho, capim-braqui?ria e Macrotyloma axillare (MBM) e cons?rcio milho, capim-braqui?ria e Stylozanthes capitata (MBS). O delineamento experimental utilizado foi inteiramente casualizado. Para cada tipo de cultivo ceifaram-se aleatoriamente cinco parcelas ou repeti??es de tr?s metros lineares e procedeu-se uma separa??o do material. As vari?veis avaliadas foram: produtividade da mat?ria seca por ?rea (PMS/ha), produtividade da MS do milho por ?rea (PMS milho-kg/ha), produ??o de prote?na bruta por ?rea (PPB-kg/ha), e produtividade de nutrientes digest?veis totais por ?rea (PNDT-kg/ha). Houve diferen?a entre as formas de cultivo para a produ??o de mat?ria seca por hectare. O milho cultivo exclusivo (MCE), n?o diferiu de milho e capim-braqui?ria (MB) e milho, capim-braqui?ria e Calopogonioum mucunoides (MBC), entretanto foi superior aos cons?rcios milho, capim-braqui?ria e Macrotyloma axillare (MBM) e milho, capim-braqui?ria e Stylosanthes captata (MBS). O material oriundo das culturas foi ensilado quando os gr?os de milho encontravam-se numa posi??o intermedi?ria na ?linha do leite?, com mat?ria-seca entre 28 e 32. Ent?o o material foi colocado e adequadamente compactado em silos baldes de polivinil carbono, nos quais foram adaptados flanges nas tampas para permitir o extravasamento de gases. Ap?s o enchimento, os baldes foram lacrados para evitar a troca de ar com o meio. Retirou-se uma amostra de cada repeti??o para a determina??o dos teores de MS, PB, extrato et?reo (EE), lignina, FDN, FDA e NDT. Os teores de MS, PB, FDN, FDA, lignina e NDT n?o variaram entre as silagens dos diferentes tratamentos. Uma fra??o de amostra de silagem de cada tratamento foi prensada para extra??o do suco e determina??o do pH, do teor de ?cido l?tico, but?rico. Os teores de ?cido l?tico n?o variaram entre os tratamentos. O teor de ?cido but?rico do MCE (0,0252% na MS) diferiu dos tratamentos MBM e MBS que apresentaram valores de 0,0358 e 0,0403% na MS, respectivamente. Todas as silagens apresentaram boa qualidade com bons padr?es de fermenta??o. No segundo experimento avaliou-se o consumo, a produ??o e composi??o do leite de vacas F1 (Holand?s x Gir) alimentadas com silagens oriundas de forragens dos diferentes cons?rcios acima relatados. Para tanto, utilizaram-se cinco vacas em mesmo per?odo de lacta??o, distribu?das em um quadrado latino balanceado (5x5). Os animais receberam as cinco dietas e concentrado comercial. O experimento foi constitu?do de cinco per?odos com dura??o de dez dias cada (sete de adapta??o ?s dietas e tr?s para coleta). Os animais foram mantidos em baias individuais, cobertas, com piso cimentado, contendo comedouros e bebedouros individuais. Foram avaliados o consumo a digestibilidade, a produ??o e a composi??o do leite das vacas. N?o foram observadas varia??es no consumo e na digestibilidade das diferentes silagens pelos animais. A produ??o e a composi??o de leite n?o diferiram entre os tratamentos. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Zootecnia, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2011. / ABSTRACT Two experiments had been conducted to evaluate the productivity and quality of forages produced on intercropping systems - corn, signal grass and various legumes. At first, there has been evaluated the yield, chemical composition and fermentation characteristics of silages of the following treatments: corn in exclusive cultivation (MCE); intercropping corn and signal grass (MB); intercropping corn, signal grass and Calopogonium mucunoides (MBC), intercropping corn, signal grass and Macrotyloma axillare (MBM) and intercropping corn, signal grass and Stylozanthes capitata (MBS). The experimental design was completely randomized. For each type of crop, there have been reaped at random five plots or replications of three linear meters and carried out a separation of the material. The variables evaluated were: dry matter yield per area (PMS/ha); yield of corn?s dry matter per area (corn PMS - kg/ha), crude protein production per unit area (PPB-kg/ha), and yield of total digestible nutrients per unit area (PNDT- kg/ha). There have been differences between the forms of cultivation for the production of dry matter per hectare. The corn in exclusive cultivation (MCE) did not differ from corn and signal grass (MB) and corn, signal grass and Calopogonioum mucunoides (MBC), however, it was higher than corn, signal grass and Macrotyloma axillare (MBM) and corn, signal grass and Stylosantes captata (MBS) intercropping. Material from crop was ensiled when maize grains was in an intermediate position in the "milk line, with dry matter between 28 and 32. Then the material was properly placed and compacted in buckets silos of polyvinyl carbon with a capacity of 12 kg of fresh material; in which were adapted silicone flanges on the covers to allow the release of gas. After filling the buckets they were tightly sealed with adhesive tape to prevent air exchange with the environment. There has been removed a sample of each replicate for the determination of MS, PB, ether extract (EE), lignin, FDN, FDA e NDT. The amount of MS, PB, FDN, FDA, lignin and NDT did not vary among silages from different treatments. The MCE?s amount of butyric acid (0.0252% of MS) differed from the MBM and MBS treatments that had values of 0.0358 and 0.0403% of dry matter, respectively. All silages presented good quality with good standards of fermentation. The second experiment evaluated the milk?s consumption, production and composition of crossbred cows F1 (Holstein x Gir) fed with silages from the different consortia reported above. To this end, there have been used the same five cows in lactation period, distributed in a balanced Latin square (5x5). The animals were fed with the five diets and with commercial concentrate. The experiment consisted of five periods lasting ten days each (seven for adaptation to diets and three for collection). They were kept in individual stalls, covered and with cemented floor, equiped with individual feeders and drinkers There have been evaluated the digestibility, production and composition of cows? milk. There hasn?t been observed variations inn the intake and digestibility of different silage. The production and milk composition did not differ between treatments.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-29T21:33:58Z No. of bitstreams: 1 JoseBarrosDaSilva_TESE.pdf: 1426605 bytes, checksum: 55de9dd327c246227a25fa3c01549654 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-30T20:04:36Z (GMT) No. of bitstreams: 1 JoseBarrosDaSilva_TESE.pdf: 1426605 bytes, checksum: 55de9dd327c246227a25fa3c01549654 (MD5) / Made available in DSpace on 2016-03-30T20:04:36Z (GMT). No. of bitstreams: 1 JoseBarrosDaSilva_TESE.pdf: 1426605 bytes, checksum: 55de9dd327c246227a25fa3c01549654 (MD5) Previous issue date: 2014-06-30 / Considerando a biodiversidade vegetal do Nordeste brasileiro, cujos componentes podem estar inseridos em sistemas de produ??o sustent?vel, o juazeiro (Ziziphus joazeiro Mart.) desponta como uma possibilidade de aproveitamento do seu fruto. O presente estudo apresenta como objetivos caracterizar o fruto do juazeiro sob o aspecto f?sico, f?sico-qu?mico e qu?mico e avaliar a sua conserva??o por fermenta??o l?ctica espont?nea, sob a influ?ncia dos cloretos de s?dio, c?lcio e pot?ssio. Conforme a legisla??o, hortali?a acidificada por fermenta??o ? aquela submetida ? fermenta??o l?tica de forma a atingir o pH do produto final igual ou menor a 4,5. Os resultados da caracteriza??o f?sica, f?sicoqu?mica e qu?mica do fruto do juazeiro maduro mostrou as potencialidades desta esp?cie para o processamento agroindustrial. O rendimento da parte comest?vel (91,83%), teor de s?lidos soluveis (18,98? Brix), acidez titul?vel (0,14% em ?cido c?trico), pH (5,30) e a sua composi??o, fracionada, em umidade (79,01%), prote?nas (2,01%), lip?dios (0,52%), carboidratos (17,59%), fibra, cinzas (0,76%) e os seus minerais foram compat?veis com o perfil caracter?stico das frutas, favorecendo, portanto, o desenvolvimento da fermenta??o l?ctica espont?nea. O pH m?nimo e a acidez titul?vel m?xima observados no processo de fermenta??o, sob a influ?ncia das misturas dos sais (NaCl, NaCl2 e KCl), variaram entre os valores de 3,4 a 3,7 e de 0,54 a 0,95 (% em ?cido l?ctico), respectivamente. O perfil da fermenta??o l?ctica do fruto do juazeiro em salmoura, a qualidade microbiol?gica do fermentado e o resultado da an?lise sensorial preliminar das conservas elaboradas, respaldaram a aplica??o da avalia??o sensorial junto ao consumidor, especificamente, da conserva derivada do fruto fermentado na presen?a do cloreto de s?dio, em acordo com as t?cnicas tradicionais de fermenta??o l?ctica de vegetais. Os resultados da avalia??o sensorial realizada com 100 consumidores (provadores) revelaram um ?ndice de aceita??o igual a 78% da conserva. Apesar de restri??es ? aceita??o sensorial das conservas fermentadas sob a influ?ncia de sais (CaCl2 e KCl) substitutos do cloreto de s?dio, estas apresentaram perspectivas de equil?brio entre a otimiza??o das misturas, a seguran?a sanit?ria dos produtos e a conscientiza??o do consumidor no sentido de preferir um produto mais saud?vel com teor de s?dio reduzido. / Considering the plant biodiversity in the Brazilian Northeast, whose components can be inserted into sustainable production systems, the jujube (Ziziphus joazeiro Mart.) emerges as to recovery of its fruit. The present study has as objective to characterize the fruit of the jujube under the physical, physicochemical and chemical approach and assess its conservation by spontaneous lactic fermentation under the influence of chloride, sodium, calcium and potassium. According to the legislation, vegetable acidified by fermentation that is subjected to lactic acid fermentation in order to achieve a final product pH less than or equal to 4.5. The results of the physical, chemical and physico-chemistry of ripe fruit jujube showed the potential of this species for agro-processing. The yield of edible portion (91.83%), soluble solids content (18,98? Brix), titratable acidity (0.14% citric acid), pH (5.30) and its composition, divided in moisture (79.01%), protein (2.01%), lipids (0.52%), carbohydrate (17.59%), fiber, ash (0.76%) and its minerals were consistent with the characteristic profile fruits, thus favoring the development of spontaneous lactic fermentation. The minimum pH and titratable acidity observed maximum in the fermentation process under the influence of mixtures of salts (NaCl and KCl NaCl2) values ranged from 3.4 to 3.7 and from 0.54 to 0.95 (% lactic acid), respectively. The profile of the lactic fermentation of fruit of jujube in brine, fermented microbiological quality and the result of analysis of primary sensory prepared preserved, the application of endorsed by the consumer sensory evaluation, more particularly, derived from fermented fruit preserved in the presence of chloride sodium, in accordance with the traditional techniques of lactic fermentation of vegetables. The results of sensory evaluation conducted with 100 consumers (tasters) revealed an acceptance rate equal to 78% of the preserve. Despite restrictions on the sensory acceptability of fermented under the influence of salts (KCl and CaCl2) substitutes sodium chloride, preserved these perspectives presented to balance the optimization of mixtures, health product safety and consumer awareness towards prefer a more healthy product with reduced sodium content.

Made available in DSpace on 2016-04-28T14:59:41Z (GMT). No. of bitstreams: 1 Almira Biazon Franca.pdf: 424732 bytes, checksum: 6b668d3884ce7bf7de269eed230cab2b (MD5) Previous issue date: 2010-03-05 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work aimed to evaluate the effects of inclusion of bakery waste (BW) in sheep diets on intake, apparent digestibility, balance of nitrogen compounds and ruminal parameters, and determine the carbohydrates and nitrogenous fractions of food and diets. Five levels of corn replacement by BW (0, 25, 50, 75 and 100%) were studied, using five male lambs with live weight of 30 kg, in a Latin square 5 X 5 design. The experimental diets were composed of concentrate and hay Tifton 85 (Cynodon spp) in a forage:concentrate ratio of 60:40. To determine the intake and digestibility were collected samples of food, orts and feces. Urine was collected in buckets for a period of 24 hours to determination of nitrogen balance. Data were interpreted with to variance analysis and regression, using the t test at 5% significance. The BW present higher value for the non fiber carbohydrate and protein fraction with fast ruminal degradation (A+B1), which brought a greater proportion of these fractions in diets replacing 100% of corn by the BW and greater timing of energy supply and protein in the rumen. There wasn t effect (P>0.05) levels of substitution of intake, digestibility of nutrients, balance of nitrogen compounds and weight gain. Different levels of substitution didn t affect (P>0.05) pH values and concentrations of volatile fatty acids, but for the concentration of ammonia nitrogen (N-NH3) was found linear reduction (P<0.05) with the level of replacement, in which each 1% BW promoted reduction of 0,11 mg/dL in the concentration of N-NH3, which may be related to increased ruminal availability of energy, which allows greater use of ammonia for microbial growth. The addition of BW caused a reduction of 51,15% in the cost of ration. It was concluded that the BW can replace the corn in concentrate rations for sheep. / Objetivou-se, com este estudo, avaliar os efeitos da inclus?o do res?duo de panifica??o (RP) na dieta de ovinos, sobre o consumo, a digestibilidade aparente, o balan?o de compostos nitrogenados e os par?metros ruminais, e determinar as fra??es de carboidratos e nitrogenadas dos alimentos e das dietas. Foram estudados cinco n?veis de substitui??o do milho pelo RP (0; 25; 50; 75 e 100%), utilizando-se cinco cordeiros machos, com peso m?dio de 30 kg, distribu?dos segundo um delineamento em Quadrado Latino 5 x 5. As dietas experimentais foram compostas de concentrado e feno de capim-Tifton 85 (Cynodon spp), numa rela??o volumoso:concentrado de 60:40. Para a determina??o do consumo e digestibilidade aparente foram coletadas amostras dos alimentos, sobras e das fezes. Foram realizadas coletas de urina por um per?odo de 24 horas para a determina??o do balan?o de compostos nitrogenados. Os resultados foram interpretados de acordo com a an?lise de vari?ncia, e regress?o, utilizando-se o teste t a 5% de signific?ncia. O RP destacou-se pelos elevados valores das fra??es de carboidratos n?o fibrosos e de prote?na de r?pida fermenta??o ruminal (A+B1), o que conferiu maior propor??o dessas fra??es nas dietas com a substitui??o de 100% do milho pelo o RP e maior sincronismo da disponibilidade de energia e prote?na no r?men. N?o houve efeito (P>0,05) dos n?veis de substitui??o sobre o consumo, digestibilidade dos nutrientes, balan?o de compostos nitrogenados e ganho de peso. Os diferentes n?veis de substitui??o n?o afetaram (P>0,05) o pH do l?quido ruminal e as concentra??es dos ?cidos graxos vol?teis, por?m para a concentra??o de nitrog?nio amoniacal (N-NH3) foi observada redu??o linear (P<0,05) em fun??o dos n?veis de substitui??o, em que cada 1% de RP promoveu redu??o de 0,11 mg/dL na concentra??o de N-NH3, o que pode estar relacionada ao aumento na disponibilidade de energia no r?men, que possibilita maior utiliza??o da am?nia para o crescimento microbiano. A adi??o de RP causou redu??o de at? 51,15% no custo da ra??o concentrada. Concluindo-se que o RP pode substituir totalmente o milho nas ra??es concentradas de ovinos.

Submitted by Verena Bastos (verena@uefs.br) on 2015-08-04T01:07:09Z No. of bitstreams: 1 TESE_Cleber Miranda Gon?alves_Vers?o Final.pdf: 4396280 bytes, checksum: c384c685c2ef38bd1e840f48280744ee (MD5) / Made available in DSpace on 2015-08-04T01:07:09Z (GMT). No. of bitstreams: 1 TESE_Cleber Miranda Gon?alves_Vers?o Final.pdf: 4396280 bytes, checksum: c384c685c2ef38bd1e840f48280744ee (MD5) Previous issue date: 2015-05-08 / Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo - FAPESP / Cacha?a corresponds to a beverage with characteristic flavor and aroma, constituted mainly of alcohol and water plus some other components formed in small amounts during the process of fermentation, distillation and aging, and known as the secondary products of alcoholic fermentation. The yeasts and fermentation conditions are considered to be the factors that influence the flavor of alcoholic beverages, since the majority of the secondary compounds responsible for the chemical and sensory quality of the beverage are formed during fermentation. The use of selected yeasts for the production of cane spirit has been studied, with a view to increasing productivity, gaining technological advantages and improving the sensory characteristics of the beverage. This study aimed to produce cacha?a in pilot and alembic scales and evaluate the chemical composition of the beverages produced by selected strains of S. cerevisiae; verify the presence of the selected yeast at the end of the fermentation process on a pilot scale and carry out the evaluation of fermentation parameters of the selected strain in relation to commercial and wild inoculums. The following 16 strains of Saccharomyces cerevisiae were tested: SC52, SC60, SC82, SC91, SC102, SC114, SC129, SC138, SC174, SC177, SC179, SC184, SC219, SC220, SC225 and SC229. Among these, only the SC82 strain could pursue multiplication and fermentation scheme steps to produce cacha?a in pilot and alembic scales. By RFLP mtDNA technique was possible to verify the permanence and dominance of selected yeast (SC82) at the end of fermentation process done in pilot scale. In the evaluation of fermentation parameters to S. cerevisiae strain SC82 had a shorter fermentation time (14h average) and a higher yield (48%), greater efficiency (93.94%) and a higher productivity (2.35 g/Lh) at the end of the third fermentation carried out in relation to commercial and wild inoculums. Regarding the results of the chemical analysis of cacha?a produced in pilot and still scale, only the higher alcohol levels were above that allowed by Brazilian legislation in both produced beverages. / A cacha?a corresponde a uma bebida dotada de sabor e aroma caracter?stico, sendo constitu?da principalmente de ?lcool e ?gua e de outros componentes, formados em pequenas quantidades durante o processo de fermenta??o, destila??o e envelhecimento, os quais recebem a denomina??o de produtos secund?rios da fermenta??o alco?lica. As leveduras e as condi??es de fermenta??o s?o apontadas como fatores que influenciam no sabor das bebidas alco?licas, pois a maioria dos compostos secund?rios respons?veis pela qualidade qu?mica e sensorial da bebida ? formada durante a fermenta??o. A utiliza??o de leveduras selecionadas para produ??o de cacha?a apresenta vantagens tecnol?gicas, como permite minimizar contamina??es indesej?veis, reduz o tempo de fermenta??o, aumenta a produtividade e melhora as caracter?sticas qu?micas e sensoriais da bebida. O presente trabalho teve como objetivos produzir cacha?a em escala piloto e de alambique e avaliar a composi??o qu?mica das bebidas produzidas por linhagens selecionadas de S. cerevisiae; verificar a presen?a da levedura selecionada no final do processo fermentativo em escala piloto e realizar a avalia??o de par?metros fermentativos da cepa selecionada em rela??o a in?culos comercial e selvagem. Foram testadas 16 cepas de Saccharomyces cerevisiae: SC52, SC60, SC82, SC91, SC102, SC114, SC129, SC138, SC174, SC177, SC179, SC184, SC219, SC220, SC225 e SC229. Destas, apenas a cepa SC82 conseguiu prosseguir as etapas do esquema de multiplica??o em escala piloto e em escala de alambique, bem como nas etapas do processo de fermenta??o em escala piloto e de alambique e produzir cacha?a. Atrav?s da t?cnica de RFLPmtDNA foi poss?vel verificar a perman?ncia e domin?ncia da levedura selecionada (SC82) no final do processo fermentativo feito em escala piloto. Na avalia??o dos par?metros fermentativos a S. cerevisiae cepa SC82 apresentou um menor tempo de fermenta??o (m?dia de 14 h) e um maior rendimento (48 %), uma maior efici?ncia (93,94 %) e uma produtividade (2,35 g/Lh) superior ao final da terceira fermenta??o realizada em rela??o ao in?culo comercial e ao in?culo selvagem. Em rela??o aos resultados das an?lises qu?micas da cacha?a produzida em escala piloto e da produzida em escala de alambique, apenas os teores de alco?is superiores estavam acima do permitido pela legisla??o brasileira nas duas bebidas produzidas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2018-04-12T13:48:46Z No. of bitstreams: 1 2010 - Onofre Dardengo.pdf: 1287779 bytes, checksum: e5433e8b536c7b1b26f3bbdc12fecc7f (MD5) / Made available in DSpace on 2018-04-12T13:48:47Z (GMT). No. of bitstreams: 1 2010 - Onofre Dardengo.pdf: 1287779 bytes, checksum: e5433e8b536c7b1b26f3bbdc12fecc7f (MD5) Previous issue date: 2010-05-03 / A group of 10 vocational students of the Federal Institute of Education, Science and Technology of Espirito Santo - IFES (Technical Course in Agribusiness) enrolled in the Module of Meat Processing was the subject of this study on the development of competences and skills in processing of salami-type sausages. The methodological approach used was qualitative and quantitative action research. The qualitative assessment was done by a preliminary diagnostic questionnaire applied before the implementation of the experiment, and a final questionnaire applied after going through the production steps, physico-chemical and microbiological analysis, and organoleptical evaluation. The approach used included lectures and hands-on processing in pilot-plant. The quantitative assessment was done through organoleptical evaluation of the processed sausages (SB=beef-only sausage; SS = pork-only sausage; SO = mutton-only sausage). The experimental model used for physico-chemical assessment was the Completely Randomized Design, distributed in a factorial 3x6 scheme, (3 types of sausage and 6 different evaluation time). The data of organoleptical evaluation was submitted to variance analysis by single-element stimuli method for acid taste, and hedonic scale for aroma, taste, overall assessment and purchase intention. The comparison of student replies to initial and final questionnaires showed that the approach was beneficial to knowledge acquisition by those involved on processing of salami-type sausages; habilities were developed to make them competent, and the combination of theory and practice was essential on constructing and reconstructing knowledge. The addition of starter culture to salami promoted the gradual downfall of pH to the 7th day (and increase in titratable acidity) due to lactic acid accumulation, condition that is basic to high quality products. Moisture content varied as function of dehydration process and length of fermentation. SB sausage scored higher in organoleptical analysis than others on taste acceptance. SB and SO sausages did not differ in aroma scores and purchase intention, both scoring higher than SS. The global assessment no significant differences were observed on all sausage types considered. On the frequency histogram analysis of scores, acceptability of SB and SO were higher than SS in all variables. Addition of bovine and ovine meat to fermented sausage of salami-type enhanced taste and aroma, and these were the product quality factors that influenced most the overall acceptability and purchase behaviour / O presente estudo foi desenvolvido no Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Esp?rito Santo ? IFES, Campus de Alegre, apresentando como sujeitos da pesquisa um grupo de 10 alunos da 2? s?rie, matriculados no M?dulo de Processamento de Carne do Curso T?cnico em Agroind?stria. Teve por objetivo promover o desenvolvimento de compet?ncias e habilidades nos alunos na produ??o de diferentes tipos de salame. O procedimento metodol?gico adotado foi a pesquisa-a??o pela abordagem qualitativa e quantitativa. A an?lise qualitativa foi avaliada utilizando-se question?rio de diagn?stico preliminar - antes da implanta??o da pesquisa e question?rio final ? ap?s a conclus?o das etapas de produ??o e participa??o na avalia??o sensorial, an?lises f?sico-qu?micas e microbiol?gicas, por meio de aulas expositivas e posteriores pr?ticas com o desenvolvimento dos produtos fermentados. J? na abordagem quantitativa, foi utilizada a an?lise sensorial para verifica??o da aceita??o dos atributos dos produtos embutidos (SB= salame de bovino; SS= salame de su?no e SO= salame de ovino). O modelo experimental adotado para avalia??o f?sico-qu?mica dos salames foi inteiramente casualizado (DIC), distribu?do em esquema fatorial 3 x 6, sendo tr?s os tipos de salame e seis as ?pocas de avalia??o. Na avalia??o sensorial, os dados foram submetidos ? an?lise de vari?ncia, pelo m?todo de est?mulo simples para sabor ?cido e da escala hed?nica para aroma, sabor, avalia??o global e inten??o de compra. As respostas dos alunos nos question?rios inicial e final permite concluir que a pesquisa contribui para a aquisi??o de conhecimento dos envolvidos no processamento de produtos embutidos tipo salame, em que foram desenvolvidas habilidades para torn?-los competentes e que a jun??o entre teoria e pr?tica foi essencial para a constru??o e a reconstru??o de saberes. A adi??o da cultura starter aos salames promoveu a queda gradual do pH at? o s?timo dia devido a libera??o de ?cido l?ctico, condi??o fundamental para a produ??o de produtos de alta qualidade. A acidez titul?vel dos salames teve um aumento significativo at? o s?timo dia da fermenta??o, cujos valores foram crescentes at? a matura??o. Os teores de umidade dos salames variaram em fun??o do processo de desidrata??o e decresceram em fun??o do tempo fermenta??o. Na an?lise sensorial o SB superou os demais para a aceita??o do sabor. N?o foram observadas diferen?as significativas para aroma e inten??o de compra entre SB e SO, que superaram SS. Na avalia??o global n?o foram observadas diferen?as significativas entre os salames avaliados. Na an?lise dos histogramas de frequ?ncia de notas, a aceitabilidade de SB e SO foi superior a de SS em todos os par?metros avaliados. A adi??o de carne bovina e ovina ao salame tipo italiano exaltou o sabor e aroma, sendo essas as propriedades que mais influenciaram na decis?o dos provadores na aceita??o global e inten??o de compra dos produtos.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-03T16:30:05Z No. of bitstreams: 1 2011 - Alex da Silva Santos.pdf: 1990293 bytes, checksum: b92b4e5aec031d9fcd17900f0f1ef9c8 (MD5) / Made available in DSpace on 2016-08-03T16:30:05Z (GMT). No. of bitstreams: 1 2011 - Alex da Silva Santos.pdf: 1990293 bytes, checksum: b92b4e5aec031d9fcd17900f0f1ef9c8 (MD5) Previous issue date: 2011-02-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES / The production of enzymes for application at different areas of food agroindustry presents promising future perspectives, due to several intrinsic properties regarded to the performance of the enzymes as natural and biodegradable compounds, responsible for achieving specific reactions with better quality. Cellulases have been the most employed enzymes on food industry, acting sinergically on the hydrolysis of the glucosidic links ?-1,4 from the molecules of cellulose, and being used on several applications in this sector as in vegetal oils extraction, fruit maceration and juice clarification. Based on this context, the present study aimed to produce, concentrate and partially characterize an enzymatic extract by a mutant fungus strain of Aspergillus niger. Production was performed by solid-state fermentation (SSF) in aerated columns, using humidified wheat bran with (NH4)2SO4 solution on 0,1N HCl as substrate and cellobiose as inducer. Cellulolytic extract was a blend of extracts from three different assays selected on previous studies as the best conditions for the enzymes caboxymethilcellulase, ?-glucosidase and filter paper cellulose (FPase). During the characterization of the enzymatic extract, besides cellulases activity, the presence of protease and other enzymes with similar action to cellulases as xylanase and poligalacturonase was evaluated. For enzymatic extract concentration, three different strategies were performed: ultrafiltration, using a stainless steel plates system through a 20 kDa molecular weight cut-off polyethersulphone membrane and 0,014 m2 area; precipitation with ammonium sulphate under 20%, 40 %, 60% and 80% saturation level and lyophiilization. The best results were achieved by the ultrafiltration process, partially purified sample and providing enzymatic activities recovery between 75% and 99%, except for FPase. SDS-PAGE analysis presented 15 visible protein bands on cellulolytic extract with molecular weights ranging from 13.3 to 104.6 kDa. Zymography test was applied for cellulases and correlate enzymes as well as to protease, however, just for the last one the conditions were considered appropriate, identifying bands on 88, 103 and 145 kDa. The effective performance of ?-glucosidase and xylanase over xylan and cellobiose hydrolysis was confirmed by thin layer chromatography. A central rotational statistical design 22 with 4 central points was used for evaluating optimal temperature and pH for carboxymethylcellulase and ?-glucosidase. The analysis of the results obtained for both enzymes demonstrated that all variables were significative at a 95% confidence level. Based on the conditions studied it can be concluded that optimal pH and temperature ranges for efficient and combined action of carboxymethylcellulase and ?-glucosidase are 3.7 to 5.5 and 60 to 65?C, respectively. / A produ??o de enzimas para uso em diferentes ?reas da agroind?stria de alimentos mostra perspectivas futuras promissoras, devido ?s v?rias caracter?sticas inerentes ? a??o das enzimas que s?o compostos naturais, biodegrad?veis e capazes de desempenhar rea??es espec?ficas com melhor qualidade. Entre as enzimas mais utilizadas pelo setor de alimentos est?o as celulases, um complexo de enzimas que atuam de forma sin?rgica sobre a hidr?lise das liga??es glicos?dicas ?-1,4 das mol?culas de celulose, e possuem v?rias aplica??es industriais neste setor, como na extra??o de ?leos vegetais, na macera??o de frutas e na clarifica??o de sucos. Dentro deste contexto, este trabalho teve como objetivo produzir, concentrar e caracterizar parcialmente um extrato celulol?tico obtido por linhagem f?ngica mutante de Aspergillus niger. A produ??o foi realizada por fermenta??o no estado s?lido (FES) em colunas aeradas, utilizando como substrato farelo de trigo triturado umidificado com solu??o de (NH4)2SO4 em HCl 0,1N e celobiose, como indutor. O extrato celulol?tico consistiu de uma mistura de extratos obtidos em 3 ensaios fermentativos diferentes, selecionados em trabalhos anteriores como as melhores condi??es para produ??o de cada uma das enzimascarboximetilcelulase (CMCase), ?-glicosidase e celulase em papel de filtro (FPase). Durante a caracteriza??o do extrato enzim?tico, al?m da atividade das celulases, tamb?m era avaliado o teor de prote?na, a presen?a de protease e de enzimas correlatas ? a??o de celulases como xilanase e poligalacturonase. Para concentra??o do extrato enzim?tico foram realizadas tr?s diferentes estrat?gias: ultrafiltra??o em um sistema de quadro e placas em a?o inox, utilizando uma membrana de polietersulfona com massa molar de corte de 20 kDa e ?rea de 0,014m2; precipita??o com sulfato de am?nio utilizando satura??es de 20%, 40%, 50%, 60%, 80% e liofiliza??o. O processo de ultrafiltra??o foi o que obteve o melhor resultado, purificando parcialmente a amostra e proporcionando uma recupera??o das atividades enzim?ticas entre 75% e 99% para todas as atividades avaliadas, exceto FPase. A an?lise eletrofor?tica em SDS-PAGE demonstrou a presen?a de 15 bandas vis?veis de prote?nas no extrato celulol?tico com pesos moleculares que compreendem uma faixa entre 13,3 e 104,6 kD. O teste de zimografia foi realizado para as celulases e enzimas correlatas, bem como para protease, no entanto somente para esta ?ltima, as condi??es testadas foram adequadas tornando-se poss?vel identificar bandas em 88, 103 e 145 kDa. A efetiva a??o das enzimas ?-glicosidase e xilanase na hidr?lise de celobiose e xilana, respectivamente,foi comprovada em cromatografia de camada fina. Al?m disso, a temperatura e pH ?timos de atua??o de carboximetilcelulase e ?-glicosidase foram determinados utilizando o delineamento composto central rotacional 22, com 4 pontos centrais. A an?lise dos resultados de ambas as enzimas demonstrou que as vari?veis eram significativas, a um n?vel de confian?a de 95%. Com base nas condi??es estudadas, concluiu-se que as faixas de pH e temperatura ?timos para a atua??o eficiente e conjunta de CMCase e ?-glicosidase est?o entre 3,7 a 5,5 e 60 a 65 ?C, respectivamente.

Made available in DSpace on 2014-12-17T15:01:45Z (GMT). No. of bitstreams: 1 SHARLINEFMS.pdf: 1463401 bytes, checksum: ffe101fa6bbf11d2b6fe49f4d837a15c (MD5) Previous issue date: 2007-12-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Pectinolytic enzymes, or simply pectinases, are complex enzymes that degrade pectic polymers. They have many uses, such as fruit juice extraction and purification, textile fiber treatment and vegetal oil extraction. The aim of this work was to study the kinetics of pectinases production by solid-state fermentation, using dry cashew apple residue as substrate and the microorganism Aspergillus niger CCT 0916. The influence of the initial medium moisture and medium supplementation with a source of nitrogen and phosphorus was evaluated using the factorial experimental planning and response surface methodology. Ammonia sulphate and potassium phosphate were used as nitrogen and phosphorus source, respectively. The variables time of contact (T) and ratio volume solvent/fermented medium (RZ), in systems with and without agitation, were evaluated in order to study the best extraction condition of the produced enzyme. Washed and unwashed cashew apple residues were tested as the growth medium. The unwashed residue was obtained by drying the residue after the extraction of the juice, while the washed residue was obtained by water washing 5 times using the proportion of 1 kg pulp/2 liters of water. Samples were taken every 12 hours for moisture content, pH, protein, reducing sugars, polygalacturonase activity (PG) and viscosity reduction. The physical-chemical composition of the residues had different sugar and pectin levels. For the unwashed residue, the peak activity was reached with 40% of initial moisture content, 1% of nitrogen supplementation without phosphorus addition after 30 hours of process. These conditions led to 16 U/g of PG activity and 82% of viscosity reduction. The calculated models reached similar values to the experimental ones in the same process conditions: 15.55 U/g of PG and 79.57% of viscosity eduction. Similarly, the greatest enzyme production for washed residue was reached with 40% initial moisture content, 1% nitrogen supplementation without phosphorus addition after 22 hours of cultivation. In this condition it was obtained polygalacturonase activity of 9.84 U/g and viscosity reduction of 81.36%. These values are close to experimental values that were of 10.1 U/g and 81%, respectively. The conditions that led to the best PG activity results was the agitated one and the best extraction condition was obtained with 100 minutes of solvent/medium contact and RZ of 5 (mL/g) / As enzimas pectinol?ticas ou pectinases formam um grupo heterog?neo de enzimas que hidrolisam as subst?ncias p?cticas, s?o usadas na extra??o de suco de fruta e sua clarifica??o, tratamento de fibra t?xtil e extra??o de ?leo vegetal. O objetivo deste trabalho foi o estudo da produ??o de pectinases (cin?tica fermentativa) atrav?s da fermenta??o em estado s?lido, usando como substrato o ped?nculo de caju seco e como agente da fermenta??o o microrganismo Aspergillus niger CCT 0916. Utilizando a metodologia do planejamento experimental fatorial e an?lise de superf?cie de resposta estudou-se a influ?ncia da umidade inicial do meio, suplementa??o do meio com fonte de nitrog?nio e fonte de f?sforo. Como fonte de nitrog?nio foi utilizado o sulfato de am?nia e como fonte de f?sforo o fosfato de pot?ssio monob?sico. Estudou-se, tamb?m, a melhor condi??o de extra??o da enzima produzida do meio de fermenta??o. Neste caso, foram estudadas as vari?veis: raz?o volume de solvente/gramas de meio fermentado e tempo de contato entre as fases, utilizando-se dois sistemas de extra??o com e sem agita??o. Como meio de cultivo foram utilizados dois res?duos do ped?nculo de caju: res?duo sem lavar e res?duo lavado. Estes res?duos diferem devido ao tratamento dado antes da secagem. O sem lavar foi obtido secando o res?duo ap?s a extra??o do suco, enquanto que o lavado, foi obtido lavando-se com ?gua, cinco vezes, ap?s a extra??o do suco, na propor??o 1 kg de baga?o para 2 litros de ?gua. A caracteriza??o f?sico-qu?mica dos res?duos mostrou composi??es diferentes, principalmente em rela??o aos teores de a??cares redutores e pectina. Durante o cultivo foram analisados, em intervalos de aproximadamente 12 horas, umidade do meio, pH, teor de prote?na, a??cares redutores, atividade da poligalacturonase (PG) e o percentual de redu??o de viscosidade. Para o res?duo sem lavar o pico de atividade foi com 40% de umidade e 1% de nitrog?nio, sem adi??o de f?sforo, com 30 horas de cultivo sendo 16 U/g de atividade de PG e 82% de redu??o de viscosidade. As equa??es emp?ricas obtidas fornecem valores bem pr?ximos aos experimentais nas mesmas condi??es de processo, 15,55 U/g de PG e 79,57% de redu??o de viscosidade para o res?duo sem lavar. Assim como para o res?duo sem lavar, para o res?duo lavado os picos de produ??o da enzima ocorreram para as mesmas condi??es de processo: umidade de 40%, nitrog?nio de 1%, sem adi??o de f?sforo, com 22 horas de cultivo. Nesta condi??o, obteve-se atividade da poligalacturonase de 9,84 U/g e percentual de redu??o de viscosidade de 81,36%. Estes valores est?o bem pr?ximos aos valores experimentais que foram de 10,1 U/g de PG e 81%, respectivamente. Na extra??o da PG o sistema que apresentou os melhores resultados de atividade foi o operado com agita??o e a melhor condi??o de extra??o foi com o tempo de contato solvente com o meio de fermenta??o de 100 minutos e a raz?o volume de solvente com o meio fermentado 5 (mL/g)