The Aristolochiaceae are one of the largest angiosperm families, the family has been divided into two subfamilies: Asaroideae, which include Asarum and Saruma, and Aristolochioideae, which includes Thottea sensu lato and Aristolochia sensu lato (Kelly and Gonzales, 2003). Aristolochia sensu lato comprise between 450 and 600 species, distributed throughout the world with centers of diversities in the tropical and subtropical regions (Neinhuis et al., 2005, Wanke et al., 2006a, 2007). However, the extended Mediterranean region including Turkey, the Caucasus and the Near East is likely to be the only diversity hotspot of the genus Aristolochia in the northern hemisphere were up to 60 species and subspecies could be observed (Wanke 2007). Most important contributions to the knowledge of these species were published by Nardi (1984, 1988, 1991, 1993) and Davis & Khan (1961, 1964, 1982), all of these studies were based on morphological characters only. In recent years, with the progress of molecular techniques and in light of the systematic chaos, a detailed study was needed to unravel the evolutionary history prior to a taxonomic revision of this group. The first chapter of my thesis should be regarded as the starting point for more detailed investigation on population level. Preliminary molecular phylogenitic analysis recovered the Mediterranean Aristolochia species as monophyletic (de Groot et al 2006). However, only very few members were included in that study. The latest phylogenetic study by Wanke (2007) dealed with west Mediterranean Aristolochia species and sampled also few members belonging to the east Mediterranean and Caucasian species (3 from Greece, 2 from Georgia and 1 from Turkey). This study reported the Mediterranean Aristolochia species as two molecular and morphologically well supported clades, which were sister to each other. Furthermore, the two closely related species A. sempervirens and A. baetica which have an east west vicariance and are known as Aristolochia sempervirens complex has been recovered as sister group to the remaining west Mediterranean species. A detailed investigation of the evolutionary history of this group is the topic of the second chapter of my thesis (Chapter 2). The Aristolochia sempervirens complex is characterized by an unusual growth form and has a circum Mediterranean distribution. The investigation of these species complex seem to be of great importance to understand speciation and colonization of the Mediterranean by the genus Aristolochia and might shade light in historical evolutionary processes of other plant lineages in the Mediterranean. Furthermore, I test applicability and phylogenetic power of a nuclear single copy gene (nSCG) region to reconstruct well resolved and highly supported gene genealogies as a prerequisite to study evolutionary biology questions in general. Furthermore, a comprehensive overview of leaf epicuticular waxes, hairs and trichomes of 54 species from the old and new world taxa of the genus Aristolochia were investigated using scanning electron microscopy (SEM) to clarify taxonomic status of theses species in contrast to their molecular position. Also this study which is the third chapter of this thesis (Chapter 3), has a strong focus on Mediterranean Aristolochia and tries to provide additional support for molecular findings based on epicuticular waxes and to test them as synapomorphies. Each chapter has its own introduction and abstract resulting in a short general introduction here.

The focus of this master's thesis is on the role of specific media in the diffusion of Japanese culture. Even though Japan is both geographically distant and culturally distinct from the rest of the world, it has become popular among fans globally. One of the main factors contributing to this fascination is a so-called media mix including Japan-specific media ranging from manga and anime to videogames. The aim of this master's thesis is to find out, based on a quantitative research conducted in both Czech and English-speaking environments, how various media outlets in Japan contribute to its popularity world-wide, identify the most important medium and scrutinize it through a descriptive analysis based on the theories of globalisation and cultural hybridisation to see what makes this medium so distinct. The author discovered that the most significant medium contributing to the diffusion of Japanese culture is anime. One of the main reasons behind its success is a commercial strategy of Japanese producers who intentionally remove cultural boundaries out of anime to make it globally acceptable. Another contributing factor connected to that, is the hybrid look of anime characters that do not have the physical features of Japanese people, which is why audiences tend to link the characters to their own...

On the surface the practice of African musics and dances in Sweden seems to be evidence of otherization and exoticization of African cultures. However, those Swedes of non-African background who do African musics and dances are genuinely engaged with the practice in the sense that they participate wholeheartedly and seem to both value and feel positive outcomes from their participation. This thesis explores how the body meets new ‘other’ musics and acquires new musicking practices, and how this is influenced by the body’s musical habitus as well as its own individual life experiences and emotional context. Additionally I look at the body’s role as an integral part of music, rather than as external to it. To illustrate this I use a case study of a group of Swedes on a cultural-eduational tourism trip to Ghana and my own first- hand experience with them learning choreographed dance based on traditional movements and rhythms, and Ewe polyrhythmic drumming. I show how discourses of difference resulting from historical contact, and previous individual experiences influence the encounter with new music, and how the body through social interactions perceives and acquires new musical practices, integrating them with known musical practices to form a kind of hybrid or new musical practice. / <p>Examensarbetet ingick i en Masterexamen i humaniora med inriktning musikvetenskap.</p>

Are municipal bath built next to Voronez Hotel able to compete with other such facilities? The key is to create a specific atmosphere. The bath steeps from the ground to offer views over city. Another part of the strategy is a presence of relative functions in the building – you can meet many activities, which you would not expect in public bath. But what is the most important: you can enjoy these activities in intimate environment or in the public space as well. For example: you can take a tea in a bar situated in the sixth floor with exclusive view over opposite hill or you can take different tea in a small informal teahouse hidden in the middle of the disposition. An all-embracing structure of differently juxtaposed reinforced concrete boxes creates a frame for this different human experience.

Notre mémoire prend pour objet d’étude les dix-sept textes brefs rassemblés dans le recueil Contes et nouvelles suivis du Théâtre, écrits durant la période symboliste et dans lesquels on constate un phénomène d’hybridation entre les genres narratif et dramatique ainsi qu’entre thèmes symbolistes et décadents. Le premier chapitre s’attache à l’étude de Contes et nouvelles suivis du Théâtre dans la perspective des poétiques du recueil afin de constituer les éléments qui en font une « anthologie de l’entre-deux » du corpus rachildien de textes brefs. En adoptant la perspective de la poétique des genres, il sera question dans le deuxième chapitre d’étudier l’hybridation des genres littéraires, plus précisément entre la prose narrative et le texte dramatique, donnant lieu à des textes en « prose dramatique » qui se situent entre texte à lire et à mettre en scène. Enfin, dans le troisième chapitre nous examinerons les thèmes et les motifs récurrents de l’imaginaire rachildien où s’entremêlent symbolisme et décadentisme, après nous être intéressée à « l’écriture artiste » sous la plume de Rachilde, qui permet de rendre « visibles » les différentes formes de spectres, d’illusions et d’ombres qui parsèment les textes de Contes et nouvelles suivis du Théâtre. / Since Rachilde is generally associated with the french Decadent mouvement, few people are informed that the author also contributed to the symbolist movement of the 1890’s by helping in the set-up of the Théâtre de l’Art and Théâtre de l’Œuvre and especially by composing plays, tales and short-stories which take part in the symbolist spirit of the late nineteenth century. Gathered in the collection Contes et nouvelles suivis du Théâtre published in 1900 by the Mercure de France, these seventeen texts written between 1890 and 1900 establish an anthology of what we call Rachilde’s "symbolist period" of short-story, tale and play writing. Our dissertation is interested in the phenomenon of hybridisation between narrative and dramatic genres as well as between decadent and symbolist themes in this collection little known amongst scholars and the general public. The first chapter of our dissertation becomes attached to the study of Contes et nouvelles suivis du Théâtre in the perspective of the poetics of the collection and examines how the combination of Rachilde’s texts leads to an "anthologie de l’entre-deux". In our second chapter, we adopt the viewpoint of the poetics of genre to discuss the hybridisation of literary genres in Contes et nouvelles suivis du Théâtre, more precisely the narrative and the dramatic genres, that result in "dramatic prose". Finally, in the third chapter, we shall examine the recurring themes of Rachilde’s writing, that entangles decadent and symbolist themes, after being interested in "le style artiste", which allows her to sketch "in a visible way" the various forms of spectres, illusions and shadows that strew the texts of Contes et nouvelles suivis du Théâtre.

House mouse hybrid zone is a complex of subspecies Mus musculus musculus, Mus musculus domesticus and their hybrids. This hybrid zone is considered as a tension zone, maintained by balance between dispersion of individuals towards the zone center and negative selection against the hybrids. Decreased anti-parasite resistance could be one of selective factors which maintain the hybrid zone. In this thesis, I use hematological methods and skin-swelling test to compare variability in mouse health state within the house mouse hybrid zone. The skin-swelling test is a method measuring pro-inflammatory immune responsiveness. Since the commonly adopted method to perform this test does not allow clear interpretation of the test results, in this thesis I also aim to optimise the test protoco . I found that utilization of concanavalin A (ConA) is more suitable in mice than application of the commonly used phytohemaglutinin (PHA). Assessment of health state of mice by both hematological methods and skin-swelling test consistently indicates increased ability of anti-parasitic resistance in the subspecies M. m. musculus compared to subspecies M. m. domesticus. Hematological examination further shows better health state of hybrid individuals compared to parental subspecies. Our results support hybrid resistance hypothesis....

L’hybridation est un processus aux conséquences diverses sur l’évolution des espèces qui est difficile à étudier lorsque les espèces se distinguent mal au niveau morphologique. Afin de comprendre le rôle de l'hybridation dans l’évolution du complexe d’espèces des chênes blancs européen, nous avons utilisé des outils de la génétique des populations pour quantifier les flux de gènes interspécifiques contemporains et étudier le système de reproduction de quatre espèces. Un protocole d'analyse génétique rapide a été développé et des méthodes d’assignations génétiques, permettant de déterminer l’espèce de chaque arbre et d’identifier les hybrides, ont été testées par simulations. Cette méthode a été appliquée en populations naturelles révélant un pourcentage d'hybrides variant de 10 à 30% en fonction des populations et impliquant tous les couples d'espèces. Nous avons montré que les effectifs des espèces dans les parcelles influencent la dynamique d'hybridation et la directionalité de l'introgression. Nous avons étudié le système de reproduction de ces espèces en croisements contrôlées et en forêt pour expliquer le maintien des espèces malgré la présence de flux de gènes interspécifiques. L'existence de plusieurs barrières reproductives contribue à un isolement partiel des espèces qui dépend principalement de barrières pré-reproductives et prézygotiques. Une analyse de paternité pratiquée sur des descendances récoltées en forêt montre que l'hybridation de première génération est rare mais que ces hybrides F1 sont fertiles et se reproduisent principalement avec l'une des espèces parentales, produisant de nombreux rétrocroisements qui expliquent le fort pourcentage d'hybrides observé dans les populations naturelles étudiées. L'hybridation et l'introgression sont donc des processus à l'œuvre chez les chênes qui contribuent à l'évolution du complexe d'espèces. / Hybridisation is a complex process with diverse consequences on species evolution. Hybridisation is difficult to study when species are not clearly morphologically distinguished. Our aim was to study the role of hybridisation in the evolution of the European white oak species complex. We used population genetic tools to quantify contemporary interspecific gene flow and to study the mating system of four oak species. A fast genetic analysis protocol was developed and genetic assignment methods were first tested by simulation and then used to determine the species of each tree and to identify hybrids. These methods revealed that hybrid percentages were between 10 to 30% depending on the natural population studied and that all species pairs were involved. We showed that the census number of species in the stands had an influence on hybridisation dynamics and on introgression direction. We studied the mating system of these species in controlled crosses and in the forest to understand the maintenance of species despite interspecific gene flow. Several reproductive barriers contribute to a partial isolation of species, mostly pre-reproductive and prezygotic. A paternity analysis of maternal progenies sampled in the forest showed that first generation hybridisation was rare but that F1 hybrids were fertile and were mating mostly with one of the two parental species, creating numerous backcrosses that explain the high percentages of hybrids observed in the natural populations studied. Hybridisation and introgression are active processes in oaks and contribute to the evolution of the species complex.

Le présent travail s’intéresse à dix pièces écrites et jouées entre 1580 et 1625. Le corpus retenu inclut des pièces rédigées par Robert Daborne, Thomas Dekker, Thomas Heywood, Christopher Marlowe, Philip Massinger, Thomas Middleton, Samuel Rowley et William Shakespeare et Nathaniel Woodes. Cette étude tâche principalement de révéler en quoi les dramaturges de la Renaissance se sont appropriés et ont transformé des codes appartenant à la tradition de la disputatio médiévale afin de mettre en scène les dissensions religieuses de leur époque. Nous avons pris en compte deux acceptions du terme « dispute ». Il peut en effet être défini comme un débat formel et dialectique et comme la manifestation d’un désaccord violent entre deux personnes ou plus.La première partie étudie les éléments conflictuels que l’on trouve dans les scènes de dispute, observant comment les dramaturges mettent en scène les différents aspects du conflit théologique et se sont emparés de l’idée d’hybridité religieuse qui caractérise la période. Cette partie s’interroge sur la mesure dans laquelle la scène de dispute reflète mais aussi nourrit le conflit religieux. Toutefois, une seconde partie analyse ces dialogues et rencontres conflictuelles, souvent violents, comme une manière paradoxale de négocier une certaine forme de coexistence et de décréter une trêve. Une troisième partie se concentre enfin sur les procédés dramatiques mis en œuvre par les auteurs pour proposer une résolution du conflit et atteindre un compromis littéraire entre une forme artistique élitiste et populaire. Ce travail souligne également le lien entre une célébration de l’art du théâtre comme un art fondamentalement hybride et la représentation du conflit religieux à travers les scènes de dispute. / This study focuses on ten plays written and performed between 1580 and 1625. The corpus includes plays by Robert Daborne, Thomas Dekker, Thomas Heywood, Christopher Marlowe, Philip Massinger, Thomas Middleton, Samuel Rowley, William Shakespeare and Nathaniel Woodes. The primary aim of this work is to determine the extent to which Renaissance dramatists appropriated and transformed the old tradition of the medieval disputatio in order to stage the religious dissensions of their time. Two definitions of the word ‘dispute’ were considered. Indeed, it can be defined both as a formal dialectical debate and as a violent disagreement between two or more people.The first part explores the conflictual elements to be found in a scene of dispute, looking at how the playwrights staged the different aspects of the conflict and dealt with the idea of religious hybridity which characterises the period. This part questions the extent to which the scene of dispute reflects but also fuels the religious feuds. Nevertheless, the second part analyses these conflictual, and sometimes violent, encounters and dialogues as a paradoxical way to negotiate a certain form of coexistence and to call a truce. Finally, a third part focuses on the way the playwrights used drama to suggest a solution to the conflict and to reach a compromise between an elitist and a popular form of art. This study also explores the link between the vindication of the art of theatre as something fundamentally hybrid and the representation of the religious conflict through scenes of dispute.

In the late 1990s, a series of landmark publications described RNA interference (RNAi) and related RNA silencing phenomena in nematodes, plants and fungi. By manipulating RNA silencing, biologists have been able to create tools for specifically inactivating genes. In organisms from trypanosomes to insects, RNA silencing is now indispensible for studying gene function. RNA silencing has been used in a project aimed at systematically knocking out all genes in the model plant Arabidopsis thaliana. RNA silencing has a natural role in defending eukaryotic cells against virus replication. By assembling virus DNA sequences in a form that triggers RNA silencing, biologists have created plants resistant to specific viruses. In this study, we set out to test if a similar approach would protect plants against infection by the agriculturally important Brassica pathogen, Plasmodiophora brassicae. P. brassicae is an obligate intracellular biotroph, from the little studied eukaryotic supergroup, the Rhizaria. To identify the gene sequences that would be starting material for P. brassicae RNA silencing, new P. brassicae genes were gathered by cDNA cloning or genomic PCR-walking. Using suppression subtractive hybridisation (SSH) and oligo-capping cloning of full-length cDNAs, 76 new gene sequences were identified. A large proportion of the cDNAs were predicted to contain signal peptides for ER translocation. In addition to the new cDNA identified here, partial sequences for the P. brassicae actin and TPS genes were published by other researchers close to the beginning of this study. Using PCR-walking, full-length genomic DNA sequences from both genes were obtained. Later, genomic DNA sequences spanning or flanking a total of 24 P. brassicae genes were obtained. The P. brassicae genes were rich in typical eukaryotic spliceosomal introns. Transcription of P. brassicae genes also appears likely to begin from initiator elements rather than TATA-box-containing promoters. A segment of the P. brassicae actin gene was assembled in hairpin format and transformed into Arabidopsis thaliana. Observation of simultaneous knockdown of the GUS marker gene as well as detection of siRNAs indicated that the hpRNA sequences induced RNA silencing. However, inoculation of these plants with P. brassicae resulted in heavy club root infection. We were unable to detect decreases in actin gene expression in the infecting P. brassicae, at either early or late stages of infection. We conclude that, within the limits of the techniques used here, there is no evidence for induction of RNA silencing in P. brassicae by in planta produced siRNAs.

<p>Human endogenous retroviruses (HERVs) represent approximately 7% of the human genome. This investigation was focused on one particular HERV, ERV3, with the main purpose of characterising its gene expression patterns and genomic distribution of ERV3-like sequences. Furthermore, this careful expression study should provide insights into the biological role of HERVs. The impact of HERVs in health and disease is not yet clarified. ERV3 is expressed as three envelope (<i>env</i>) transcripts, of which two also contain a cellular gene, <i>H-plk</i> (human proviral linked <i>Krüppel</i>). ERV3 <i>env</i> expression was mainly investigated at the RNA level. The gene expression of two other HERVs, HERV-K and HERV-E was analysed and compared with ERV3 activity.</p><p>Real-time PCRs were developed and in combination with in situ hybridisation, it was found that ERV3 is expressed in a tissue- and cell-specific way. High levels of ERV3 mRNA (up to six times over Histone3.3) were demonstrated in placenta, sebaceous glands, foetal and adult adrenal glands, brown adipose tissue, corpus luteum, pituitary gland, thymus and testis. In monocytic cells including both normal monocytes and malignant U-937 cells, elevated mRNA levels were observed after retinoic acid (RA)-induced differentiation. ERV3-encoded Env protein was detected in selected cases, one following RA-treatment. In addition, several new ERV3-like sequences were discovered in the human genome. </p><p>ERV3 was found to have conserved open reading frames in contrast to other ERV3-like sequences in the human genome. This suggests that ERV3 may be involved in important cellular processes such as differentiation, cell fusion, immunomodulation and protection against infectious retroviruses. The developed techniques and obtained results will allow further studies of HERV expression to better correlate HERV activity to both normal development and disease. </p>