字彙學習是語文能力重要的一環，許多教學也都是以提升孩童單字量之目的設計的，其中，故事閱讀活動就被推崇為是能以提供豐富情境的方式來增進孩童語文能力的有效策略。本研究主旨在探討繪本共讀對EFL國小學童字彙學習的影響，主要的研究方向有：(1) 圖文標指與引導問答教學對學童運用繪本學習字彙的影響; (2)不同教學引導方式對學生字彙保留量的影響; (3)不同教學引導方式對不同程度學生所產生的影響。 受試者為五十四位台北縣五年級國小學童，其字彙測驗成績則為資料分析來源。本研究主要發現有下列幾點。第一，圖文標指和引導問答教學對國小學童運用繪本學習字彙皆有助益。第二，圖文標指和引導問答教學對學童字彙保留量都有正面的影響。第三，英語學習高成就學生在運用繪本學習字彙上收穫比低成就學生多，其中，圖文標指教學對高成就以及低成就學生皆有助益；此外，總括來說，引導問答教學對高成就學生的字彙學習幫助最大。 最後，本文提供了教學者以及日後研究者相關建議，並期盼研究結果能帶給台灣英語老師一些字彙教學上的參考。 / Vocabulary plays an important role in children’s emergent literacy. Many instructions were developed to build children’s word knowledge. Among them, storybook reading is promoted to be an effective strategy that enhances children’s literacy by providing rich contexts. The purpose of the present study aims to examine the effects of the techniques of labeling (L) and labeling plus questioning (L+Q) for vocabulary teaching with storybook reading in EFL elementary school. The study is mainly concerned with three aspects: (1) the effects of L and L+Q on student’s vocabulary learning during shared storybook reading; (2) student’s retention of learned vocabulary affected by the two techniques; (3) the influence of the two techniques on students with diverse English proficiency. Participants were 54 fifth-grade elementary school students in Taipei County, and the data analysis was based on their performance of the Picture Vocabulary Test (PVT). The findings of the study are as follows. First, L and L+Q techniques were both beneficial for student’s receptive vocabulary learning during shared storybook reading. Second, both L and L+Q techniques were effective in retaining student’s word knowledge. Third, overall performance of students with high English proficiency (HEP) in PVT improved more significantly than that of students with low English proficiency (LEP). Besides, L technique was helpful for HEP and LEP’s receptive vocabulary learning, but not for HEP’s word retention. Moreover, HEP were most influenced by L+Q technique in vocabulary learning during shared storybook reading. Based on the findings of the present study, pedagogical implications and suggestions for future study were provided at the end of the thesis.

Il existe un lien étroit entre la structure tridimensionnelle et la fonction cellulaire de l’ARN. Il est donc essentiel d’effectuer des études structurales de molécules d’ARN telles que les riborégulateurs afin de mieux caractériser leurs mécanismes d’action. Une technique de choix, permettant d’obtenir de l’information structurale sur les molécules d’ARN est la spectroscopie RMN. Cette technique est toutefois limitée par deux difficultés majeures. Premièrement, la préparation d’une quantité d’ARN nécessaire à ce type d’étude est un processus long et ardu. Afin de résoudre ce problème, notre laboratoire a développé une technique rapide de purification des ARN par affinité, utilisant une étiquette ARiBo. La deuxième difficulté provient du grand recouvrement des signaux présents sur les spectres RMN de molécules d’ARN. Ce recouvrement est proportionnel à la taille de la molécule étudiée, rendant la détermination de structures d’ARN de plus de 15 kDa extrêmement complexe. La solution émergeante à ce problème est le marquage isotopique spécifique des ARN. Cependant, les protocoles élaborées jusqu’à maintenant sont très coûteux, requièrent plusieurs semaines de manipulation en laboratoire et procurent de faibles rendements. Ce mémoire présente une nouvelle stratégie de marquage isotopique spécifique d’ARN fonctionnels basée sur la purification par affinité ARiBo. Cette approche comprend la séparation et la purification de nucléotides marqués, une ligation enzymatique sur support solide, ainsi que la purification d’ARN par affinité sans restriction de séquence. La nouvelle stratégie développée permet un marquage isotopique rapide et efficace d’ARN fonctionnels et devrait faciliter la détermination de structures d’ARN de grandes tailles par spectroscopie RMN. / The tridimensional structure of a given RNA molecule is closely linked to its cellular function. For this reason, it is crucial to study the structure of RNA molecules, such as riboswitches, to characterize their mechanism of action. To do so, NMR spectroscopy is often used to gather structural data on RNA molecules in solution. However, this approach is limited by two main difficulties. First, the production of preparative quantities of natively folded and purified RNA molecules is a long and tedious process. To facilitate this step, our laboratory has developed an RNA-affinity purification method using an ARiBo tag. The second limiting step comes from the extensive signal overlap detected on NMR spectra of large RNA molecules. This overlap is proportional to the length of the RNA, which often prevents high-resolution structure determination of RNAs larger than 15 kDa. To solve this problem, specific isotopic labeling of RNAs can now be achieved. However, existing labeling protocols are expensive, require several weeks of laboratory manipulations and usually provide relatively low yields. This thesis provides an alternative strategy to achieve specific isotopic labeling of RNA molecules, based on the ARiBo tag affinity purification technique. The protocol includes the separation and the purification of isotopically labeled nucleotides, an enzymatic ligation step performed on a solid support and the affinity purification of the RNA of interest, without any sequence restriction. This new strategy is a fast and efficient way to label functional RNAs isotopically and should facilitate NMR structure determination of large RNAs.

No description available.

Mémoire numérisé par la Division de la gestion de documents et des archives de l'Université de Montréal.

Il existe un lien étroit entre la structure tridimensionnelle et la fonction cellulaire de l’ARN. Il est donc essentiel d’effectuer des études structurales de molécules d’ARN telles que les riborégulateurs afin de mieux caractériser leurs mécanismes d’action. Une technique de choix, permettant d’obtenir de l’information structurale sur les molécules d’ARN est la spectroscopie RMN. Cette technique est toutefois limitée par deux difficultés majeures. Premièrement, la préparation d’une quantité d’ARN nécessaire à ce type d’étude est un processus long et ardu. Afin de résoudre ce problème, notre laboratoire a développé une technique rapide de purification des ARN par affinité, utilisant une étiquette ARiBo. La deuxième difficulté provient du grand recouvrement des signaux présents sur les spectres RMN de molécules d’ARN. Ce recouvrement est proportionnel à la taille de la molécule étudiée, rendant la détermination de structures d’ARN de plus de 15 kDa extrêmement complexe. La solution émergeante à ce problème est le marquage isotopique spécifique des ARN. Cependant, les protocoles élaborées jusqu’à maintenant sont très coûteux, requièrent plusieurs semaines de manipulation en laboratoire et procurent de faibles rendements. Ce mémoire présente une nouvelle stratégie de marquage isotopique spécifique d’ARN fonctionnels basée sur la purification par affinité ARiBo. Cette approche comprend la séparation et la purification de nucléotides marqués, une ligation enzymatique sur support solide, ainsi que la purification d’ARN par affinité sans restriction de séquence. La nouvelle stratégie développée permet un marquage isotopique rapide et efficace d’ARN fonctionnels et devrait faciliter la détermination de structures d’ARN de grandes tailles par spectroscopie RMN. / The tridimensional structure of a given RNA molecule is closely linked to its cellular function. For this reason, it is crucial to study the structure of RNA molecules, such as riboswitches, to characterize their mechanism of action. To do so, NMR spectroscopy is often used to gather structural data on RNA molecules in solution. However, this approach is limited by two main difficulties. First, the production of preparative quantities of natively folded and purified RNA molecules is a long and tedious process. To facilitate this step, our laboratory has developed an RNA-affinity purification method using an ARiBo tag. The second limiting step comes from the extensive signal overlap detected on NMR spectra of large RNA molecules. This overlap is proportional to the length of the RNA, which often prevents high-resolution structure determination of RNAs larger than 15 kDa. To solve this problem, specific isotopic labeling of RNAs can now be achieved. However, existing labeling protocols are expensive, require several weeks of laboratory manipulations and usually provide relatively low yields. This thesis provides an alternative strategy to achieve specific isotopic labeling of RNA molecules, based on the ARiBo tag affinity purification technique. The protocol includes the separation and the purification of isotopically labeled nucleotides, an enzymatic ligation step performed on a solid support and the affinity purification of the RNA of interest, without any sequence restriction. This new strategy is a fast and efficient way to label functional RNAs isotopically and should facilitate NMR structure determination of large RNAs.

This thesis deals with the legislation of viticulture and winemaking in the Czech Republic. The main goal of this thesis is to introduce to the readers both, professionals and non-professionals, viticulture and wine law in its full extensity. For this reason, we deeply analyze the Act No. 321/2004 describing viticulture and winemaking as amended by subsequent law. The thesis is composed of seven main chapters. The abbreviations, witch are subsequently used in the work, are defined in the separate chapter. The first part is an introduction to the topic and describes the origin of growing grape-wine and winemaking. The second part deals with the historical development of vineyard and wine law in the Czech Republic. For the sake of clarity, second part is divided into five subsections. The third chapter defines the position of vineyard and wine law in legal system of Czech Republic and characterizes this specific agriculture sector. The fourth part provides a review of written sources of vineyard and wine law. The chapter is hierarchically separated into the categories: international law, EU law and Czech law. Part five is major and it is the most extensive part of this diploma. It deals with the current legislation of viticulture and winemaking in the Czech Republic by Act No. 321/2004 Coll. It...

Introdução: O papel da rotulagem como instrumento de promoção da alimentação saudável depende da adequação de sua linguagem aos objetivos da política pública. Objetivos: Revisar a literatura científica sobre a comunicação com o consumidor por meio da rotulagem nutricional e da rotulagem relativa a impactos socioambientais em embalagens de alimentos; entender como se formam os discursos sobre alimentação saudável na rotulagem; e elaborar propostas para fortalecer o efeito dos rótulos como instrumento de promoção da alimentação saudável. Métodos: Busca não sistemática de estudos sobre a comunicação via rótulos de alimentos em bases de dados do campo da saúde e multidisciplinares; e análise qualitativa do discursode embalagens de \"cereais matinais\", selecionadas a partir do ethos da marca. Resultados e discussão: A revisão traz estudos de recepção que apontam um mau aproveitamento dos rótulos pelos consumidores devido à linguagem excessivamente técnica; poucos estudos de emissão exploram o efeito de elementos visuais e verbais com apelo emocional na comunicação. A análise empírica do corpus, delimitado por quatro ethe (imagens de si criadas pelas marcas), mostra que o design das embalagens modula a geração dos sentidos, criando uma hierarquia para as mensagens capaz de reduzir o efeito das informações obrigatórias. Propõem-se novos caminhos para melhorar a comunicação sobre alimentação saudável e sustentável nos rótulos. Conclusão:A comunicação sobre aspectos nutricionais e socioambientais da alimentação via rótulos, tal como prevista na legislação atual, prejudica seu papel como instrumento de promoção da alimentação saudável. Para transmitir as mensagens conforme o pretendido pela Política Nacional de Alimentação e Nutrição, é preciso rever as informações priorizadas, repensar o design e aprimorar a linguagem, a fim de controlar melhor os efeitos de sentidos gerados. / Introduction: The role of labeling as a tool for promoting healthy eating depends on the adequacy of their language to the objectives of public policy. Objective: To review the scientific literature on communication with the consumer through nutritional labeling and the labeling related to social-environmental impacts in food packages; to understand how the discourses about healthy eating are formed in food labeling; and to develop proposals to strengthen the effect of food labels as a tool for promoting healthy eating. Methods: Nonsystematic search of studies on communication in food labels in health and multidisciplinary databases; and qualitative discourse analysis of the labels of \"cereals\", selected from the ethos of the brand. Results and discussion: The review brings reception studies that indicate a poor use of labels by consumers due to excessively technical language; few studies explore the effect of visual and verbal elements with emotional appeal in communication. The empirical analysis of the corpus, bounded by four ethe (images of themselves created by the brands), shows that the design of the package modulates the generation of meaning, creating a hierarchy of messages that is able to reduce the effect of mandatory information. Alternatives for the communication about healthy and sustainable eating in food labels are suggested. Conclusion: The communication on nutritional and social-environmental aspects of food in food labels, according to legislation, jeopardizes its role as an instrument for promoting healthy eating. To convey the message as intended by the National Food and Nutrition Policy, it is recommended to review the prioritized information in labels, to rethink the design and to enhance the language in order to better monitor the effects of meanings generated.

Um Projeto Temático de Pesquisa foi desenvolvido no Laboratório de Poluição Ambiental do Departamento de Patologia da Faculdade de Medicina da Universidade de São Paulo com o objetivo de avaliar os efeitos da exposição aguda/crônica ao ar ambiente de um grande centro urbano sobre a saúde. Dentro deste projeto, uma linha de pesquisa foi dedicada ao estudo dos efeitos dessa exposição sobre a saúde reprodutiva feminina. Evidências de estudos epidemiológicos e experimentais implicam os fatores ambientais na infertilidade humana e resultado obstétrico adverso. Contudo, poucos estudos foram conduzidos até o presente para avaliar um possível efeito da exposição à poluição ambiental particulada sobre a saúde reprodutiva feminina. Portanto, o objetivo dos projetos da minha linha de pesquisa é fornecer dados que possam demonstrar os possíveis efeitos da exposição crônica à poluição ambiental particulada sobre a função ovariana e o desenvolvimento embrionário inicial. O objetivo do primeiro projeto desta tese foi avaliar diferentes metodologias utilizadas para a coloração diferencial das linhagens celulares do blastocisto, um método mais adequado para a avaliação de sua qualidade e normalidade. As células de blastocistos intactos de camundongo obtidos através de fertilização in vitro (FIV) foram permeabilizadas e coradas utilizando-se diferentes concentrações de um detergente (TX-100; 0,5% ou 1%) e de iodeto de propídeo (IP; 50 g/mL ou 100 g/mL) e depois disso, incubadas durante a noite em uma solução contendo diferentes fixadores (etanol, metanol, paraformaldeído PFA1% ou 4%) e bisbenzimida. Para a avaliação da qualidade de coloração e contagem diferencial dos núcleos, os blastocistos foram montados em lâminas de vidro e analisados em um microscópio de epifluorescência. O escore de qualidade de coloração foi significativamente diferente (p<0,05) entre todas as soluções fixadoras, sendo maior para o etanol, seguido pelo metanol, PFA1% e PFA4%. Mudanças da concentração do IP e o uso de diferentes soluções de fixação revelaram efeitos significativos na contagem de células da massa celular interna (MCI) e na razão MCI/trofectoderma (TE). Concentrações diferentes do detergente utilizado para a permeabilização celular apresentaram efeitos significativos sobre a contagem de células TE e razão MCI/TE. Concluímos que o protocolo que utiliza TX-100 1% para a permeabilização celular, 50 g/mL de IP para coloração das células TE e etanol como solução de fixação representa o método mais eficiente para a coloração diferencial e contagem das células das linhagens celulares do embrião no estágio de blastocisto. No segundo projeto que compõe esta, o objetivo foi avaliar os efeitos da exposição pré e/ou pós-natal ao ar ambiente sobre a fertilização, desenvolvimento embrionário e segregação das linhagens celulares em blastocistos pré-implantacionais, utilizando o modelo de FIV de camundongo. Fêmeas de camundongo com idade de seis semanas tiveram a ovulação estimulada e foram expostas no período pré- e/ou pós-natal ao ar filtrado (AF-AF), ar filtrado ar ambiente (AF-AA) ou ar ambiente ar ambiente (AA-AA) em câmaras de exposição 24 horas por dia, sete dias na semana, durante nove semanas. Os pontos de avaliação reprodutivos analisados incluíram a duração da gestação, tamanho e peso da prole, índice de nascidos vivos, razão sexual, resposta ovariana à estimulação, taxa de fertilização, desenvolvimento embrionário, taxas de formação e de eclosão dos blastocistos, contagem celular total e proporção da alocação celular à MCI e TE. A duração da gestação, tamanho e peso da prole, índice de nascidos vivos e razão sexual foram similares nos diferentes grupos de exposição. A resposta ovariana não foi afetada pelo protocolo de exposição. Um efeito multivariável para a exposição pré e/ou pós-natal ao material particulado fino ambiente sobre a FIV, o desenvolvimento embrionário e a coloração diferencial dos blastocistos foi observado. A contagem celular na MCI e a razão MCI/TE dos blastocistos produzidos no protocolo AF-AF foram significativamente maiores do que aquelas em blastocistos produzidos nos protocolos FA-AA e AA-AA. Nenhuma diferença na contagem celular total foi observada. Baseando-se nessas observações, nosso estudo sugere que a exposição ao material particulado fino presente no ar ambiente de um grande centro urbano pode afetar negativamente a saúde reprodutiva feminina através da alteração da especificação das linhagens celulares do embrião no estágio de blastocisto. Finalmente, o propósito do terceiro projeto que compõe esta tese foi de avaliar os efeitos da exposição pré e/ou pósnatal ao ar ambiente no final da vida reprodutiva sobre a fertilização, desenvolvimento embrionário e segregação das linhagens celulares em blastocistos pré-implantacionais, utilizando o modelo de FIV de camundongo. Fêmeas de camundongo com idade de cinco meses tiveram a ovulação estimulada e foram expostas no período pré e/ou pós-natal ao ar filtrado (AF-AF), ar filtrado ar ambiente (AF-AA) ou ar ambiente ar ambiente (AA-AA) em câmaras de exposição 24 horas por dia, sete dias na semana, durante seis meses. Os pontos de avaliação reprodutivos foram os mesmos que aqueles utilizados no segundo projeto. A duração da gestação, tamanho e peso da prole, índice de nascidos vivos e razão sexual foram similares nos diferentes grupos de exposição. A resposta ovariana não foi afetada pelo protocolo de exposição. Um efeito multivariável para a exposição pré e/ou pós-natal ao material particulado fino ambiente sobre coloração diferencial dos blastocistos, mas não sobre a FIV e o desenvolvimento embrionário, foi observado. A contagem celular na MCI e a razão MCI/TE dos blastocistos produzidos no protocolo AF-AF foram significativamente maiores do que aquelas em blastocistos produzidos nos protocolos FA-AA e AA-AA. A contagem celular do TE dos blastocistos produzidos no protocolo FA-FA foi significativamente menor do que aquela em blastocistos produzidos nos protocolos FA-AA e AA-AA. A contagem celular total foi similar entre os grupos. Nosso estudo sugere que a exposição à poluição ambiental particulada de um grande centro urbano não altera a função ovariana, mas pode afetar negativamente a saúde reprodutiva feminina no período final do menacme, através da alteração da especificação das linhagens celulares do embrião no estágio de blastocisto / A thematic research project to evaluate the health effects of acute/chronic exposure to ambient air in a large urban center was developed at the Air Pollution Laboratory in the Department of Pathology at the University of São Paulo School of Medicine. Within this project a specific research line was committed to the study of the effects of this exposure on female reproductive health. Evidence from epidemiological and experimental studies implied environmental factors as possible contributors to human infertility and poor obstetric outcome. However, very few studies evaluating a possible effect of exposure to particulate air pollution on female reproductive health have been conducted so far. Thus, the aim of the projects in my research line was to provide data that could show the possible effects of chronic exposure to particulate air pollution on ovarian function and early embryo development. The objective of the first project was to assess different methodologies used in cell lineage differential staining of the blastocyst, a method for more accurate evaluation of its quality and normality. Cells of zona-intact mouse blastocysts obtained from in vitro fertilization (IVF) were permeabilized and stained using different concentrations of a detergent (TX-100; 0.5% or 1%) and propidium iodide (PI; 50 g/mL or 100 g/mL) followed by overnight incubation in a solution containing different fixatives (ethanol, methanol, paraformaldehyde - PFA 1% or 4%) and bisbenzimide. To evaluate the staining quality and count the nuclei differentially, blastocysts were mounted and viewed using epifluorescence microscopy. Staining quality scores were significantly different (P < 0.05) among all fixative solutions with the highest for ethanol followed by methanol, PFA1%, and PFA4%. Changes in PI concentration and use of different fixative solutions revealed significant effects on inner cell mass (ICM) cell count and ICM/trophectoderm (TE) ratio. Different concentrations of the detergent used for cell permeabilization showed significant effects on TE cell counts and ICM/TE ratio. I concluded that the protocol using 1% TX-100 for cell permeabilization, 50 g/mL of PI for TE cell staining, and ethanol as a fixative solution is the most efficient method for cell lineage differential staining and counting at the blastocyst stage. In the second project the objective was to evaluate the effects of preand/ or postnatal exposure to ambient air on fertilization, embryo development, and cell lineage segregation in preimplantation blastocysts using the IVF mouse model. Six-week old superovulated mice were preand/ or postnatally exposed to filtered air (FA-FA), filtered-ambient air (FAAA), or ambient air (AA-AA) in exposure chambers 24/7 for nine weeks. Reproductive endpoints evaluated included gestation length, litter size, litter birth weight, live birth index, sex ratio, ovarian response to superovulation, fertilization rate, embryo development, blastocyst and hatching rates, total cell count, and proportion of cell allocation to ICM and TE. Gestation length, litter size, litter birth weight, live birth index, and sex ratio were similar among exposure groups. Ovarian response was not affected by the exposure protocol. A multivariate effect for pre- and/or postnatal exposure to ambient fine particulate matter on IVF, embryo development, and blastocyst differential staining was found. Cell counts in ICM and ICM/TE ratios in blastocysts produced in the FA-FA protocol were significantly higher than in blastocysts produced in FA-AA and AA-AA protocols. No difference in the total cell count was observed. Based on these observations the study suggests that exposure to ambient fine particulate matter in a large urban center may negatively affect female reproductive health by disrupting the lineage specification at the blastocyst stage. Finally, the purpose of the third project was to evaluate the effects of pre- and/or postnatal exposure to particulate air pollution on fertilization, embryo development, and cell lineage segregation in preimplantation blastocysts during the late-life reproductive period using the IVF mouse model. Five-month-old superovulated mice were pre- and/or postnatally exposed to filtered air (FA-FA), filtered-ambient air (FA-AA), or ambient air (AA-AA) in exposure chambers 24/7 during six months. Reproductive endpoints were the same as the ones selected for the second project. Gestation length, litter size, litter birth weight, live birth index, and sex ratio were similar among exposure groups. Ovarian response was not affected by the exposure protocol. A multivariate effect for pre- and/or postnatal exposure to ambient air on blastocyst differential staining but not on IVF and embryo development was found. Cell counts in ICM and ICM/TE ratios in blastocysts produced in the FA-FA protocol were significantly higher than in blastocysts produced in FA-AA and AA-AA protocols. Cell counts in TE cells in blastocysts produced in the FA-FA protocol were significantly lower than in blastocysts produced in FA-AA and AA-AA protocols. The total cell count was similar among groups. This study suggests that exposure to particulate air pollution in a large urban center has no effect on ovarian function but may negatively affect female reproductive health in the late-life period by disrupting the lineage specification at the blastocyst stage.

The thesis deals with the influence of marketing on the customer in the choice of food. It examines whether the influence of marketing prevails over customer´s own judgment about the choice of appropriate food for daily use in terms of monitoring its nutritional composition. The theoretical part deals with the basics of marketing, with a lot of attention being paid to food goods, food labeling, nutrition and the quality of food products, which can greatly affect the quality of life. The research was conducted through a questionnaire survey. The obtained data are processed in the form of tables and graphs, supplemented with verbal commentary. The validity of the set hypotheses is statistically evaluated and processed. Based on the results of the research, at the end of the thesis, the factors influencing the purchasing decision and the individual preferences of the respondents in the food selection are evaluated. The appendix provides an overview of practical information and general advice that should help shoppers to make choices when purchasing basic foods and to better understand the information given on individual product packaging.

A grande capacidade de disponibilização de informações que a Web possibilita se traduz em múltiplas possibilidades e oportunidades para seus usuários. Essas pessoas são capazes de acessar conteúdos provenientes de todas as partes do planeta, independentemente de onde elas estejam. Mas essas possibilidades não são estendidas a todos, sendo necessário mais que o acesso a um computador e a Internet para que sejam realizadas. Indivíduos que apresentem necessidades especiais (deficiência visual, cognitiva, dificuldade de locomoção, entre outras) são privados do acesso a sites e aplicações web que façam mal emprego de tecnologias web ou possuam o conteúdo sem os devidos cuidados para com a acessibilidade. Um dos grupos que é privado do acesso a esse ambiente é o de pessoas com dificuldade de leitura (analfabetos funcionais). A ampla utilização de recursos textuais nas aplicações pode tornar difícil ou mesmo impedir as interações desses indivíduos com os sistemas computacionais. Nesse contexto, este trabalho tem por finalidade o desenvolvimento de tecnologias assistivas que atuem como facilitadoras de leitura e compreensão de sites e aplicações web a esses indivíduos (analfabetos funcionais). Essas tecnologias assistivas utilizam recursos de processamento de língua natural visando maximizar a compreensão do conteúdo pelos usuários. Dentre as técnicas utilizadas são destacadas: simplificação sintática, sumarização automática, elaboração léxica e reconhecimento das entidades nomeadas. Essas técnicas são utilizadas com a finalidade de promover a adaptação automática de conteúdos disponíveis na Web para usuários com baixo nível de alfabetização. São descritas características referentes à acessibilidade de aplicações web e princípios de design para usuários com baixo nível de alfabetização, para garantir a identificação e entendimento das funcionalidades que são implementadas nas duas tecnologias assistivas resultado deste trabalho (Facilita e Facilita Educacional). Este trabalho contribuiu com a identificação de requisitos de acessibilidade para usuários com baixo nível de alfabetização, modelo de acessibilidade para automatizar a conformidade com a WCAG e desenvolvimento de soluções de acessibilidade na camada de agentes de usuários / The large capacity of Web for providing information leads to multiple possibilities and opportunities for users. The development of high performance networks and ubiquitous devices allow users to retrieve content from any location and in different scenarios or situations they might face in their lives. Unfortunately the possibilities offered by the Web are not necessarily currently available to all. Individuals who do not have completely compliant software or hardware that are able to deal with the latest technologies, or have some kind of physical or cognitive disability, find it difficult to interact with web pages, depending on the page structure and the ways in which the content is made available. When specifically considering the cognitive disabilities, users classified as functionally illiterate face severe difficulties accessing web content. The heavy use of texts on interfaces design creates an accessibility barrier to those who cannot read fluently in their mother tongue due to both text length and linguistic complexity. In this context, this work aims at developing an assistive technologies that assists functionally illiterate users during their reading and understanding of websites textual content. These assistive technologies make use of natural language processing (NLP) techniques that maximize reading comprehension for users. The natural language techniques that this work uses are: syntactic simplification, automatic summarization, lexical elaboration and named entities recognition. The techniques are used with the goal of automatically adapting textual content available on the Web for users with low literacy levels. This work describes the accessibility characteristics incorporated into both resultant applications (Facilita and Educational Facilita) that focus on low literacy users limitations towards computer usage and experience. This work contributed with the identification of accessibility requirements for low-literacy users, elaboration of an accessibility model for automatizing WCAG conformance and development of accessible solutions in the user agents layer of web applications