Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-08-01T14:08:23Z No. of bitstreams: 1 CamilaSilveiraDuarte_DISSERT.pdf: 3812415 bytes, checksum: a177153446220a3a3c360b24320e037a (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-08-08T11:21:14Z (GMT) No. of bitstreams: 1 CamilaSilveiraDuarte_DISSERT.pdf: 3812415 bytes, checksum: a177153446220a3a3c360b24320e037a (MD5) / Made available in DSpace on 2017-08-08T11:21:14Z (GMT). No. of bitstreams: 1 CamilaSilveiraDuarte_DISSERT.pdf: 3812415 bytes, checksum: a177153446220a3a3c360b24320e037a (MD5) Previous issue date: 2017-02-22 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Esta pesquisa tem como intuito apresentar uma reflex?o acerca da trajet?ria art?stico-biogr?fica da artista afeg? Lida Abdul (1973), a partir da an?lise de tr?s de suas obras: ?In Transit? (2008), ?Military / Body? (2004) e ?Global Porn? (2002), nas quais levanta quest?es e aborda temas sobre interven??es art?sticas em territ?rios de conflito, rela??es entre corpo e viol?ncia, e reflex?es sobre a dial?tica entre o sistema capitalista e performances pol?ticas, respectivamente. Tamb?m ser?o discutidos, em conson?ncia com as obras da artista, os conceitos de desterritorializa??o e reterritorializa??o, identidades m?veis, e fronteiras perme?veis entre as linguagens art?sticas, tendo como objeto direto para esse di?logo: a Performance Art.

I den här essän utforskas en händelse där en psykoterapeut under ett samtal upplever sig drabbad i förtvivlan av två ögonblick i ett professionellt samtal. Mot bakgrund av den händelsen avtäcks betydelsen och meningsfullheten i att lida, specifikt som en möjlig färdighet hos psykoterapeuten. Det görs genom utforskning av vad att förstå, vara receptiv och erfara förtvivlans utsatthet kan betyda för en psykoterapeuts kunnande med hjälp av filosofen Søren Kierkegaard. I essän lyfts inledningsvis problemet mellan naturvetenskapens strävan att säkerställa våra liv genom diagnostiska termer och det existentiella mänskliga villkoret att lida fram. Genom utforskandet tydliggörs hur den naturvetenskapliga synen på människan subtilt förskjutit ett lidande till något som i vissa fall först och främst ges namn via en diagnos eller med annan terminologi och med det förlorar en egen legitimitet men som även gör oss sämre förberedda att lida. En fråga som ställs och ges svar på är om en psykoterapeuts färdighet att lida indirekt kan legitimera lidande genom just den färdigheten. Kierkegaards texter används för att både förstå och ge lidande dels den legitimitet som lidande bör få dels se lidande som en stilla revolutionär handling genom hans blick. Ett avgörande moment som visade sig ta psykoterapeuten från förmågan att lida till färdigheten att lida var tigande. / This essay explores an incident in which a psychotherapist during a conversation finds herself struck with despair by two moments in a professional conversation. Against the background of that event, the meaning and meaningfulness of suffering is revealed, specifically as a possible skill of the psychotherapist. It is done by exploring what understanding, being receptive and experiencing the vulnerability of despair can mean for a psychotherapist's skills with the help of the philosopher Søren Kierkegaard. The essay initially raises the problem between natural science's endeavor to secure our lives through diagnostic terms and the existential human condition of suffering. Through the research, it is made clear how the natural scientific view of man has subtly shifted suffering to something that in some cases is first and foremost given a name via a diagnosis or with other terminology and with that loses its own legitimacy, but which also makes us less prepared to suffer. A question that is asked and answered is whether a psychotherapist's skill in suffering can indirectly legitimize suffering through that very skill. Kierkegaard's texts are used to both understand and give suffering the legitimacy that suffering should have and see suffering as a quiet revolutionary act through his eyes. A decisive moment that proved to take the psychotherapist from the ability to suffer to the skill to suffer was silence.

Made available in DSpace on 2014-12-17T15:42:13Z (GMT). No. of bitstreams: 1 ValdeliceRS_TESE.pdf: 2586359 bytes, checksum: e3d9d5d8e2c97ad0990f43fd755545a2 (MD5) Previous issue date: 2010-11-26 / One of the main applications of methane is in the production of syngas, a mixture of hydrogen and carbon monoxide. Procedures used in this process are steam reforming, CO2 reforming, partial oxidation and autothermal reforming. The present study evaluated and compared the behavior of nickel catalysts supported on mixed oxides of cerium and manganese in the partial oxidation of methane with that of nickel catalysts supported on mixed oxides of cerium and zirconium. Mixed oxides of cerium and zirconium or cerium and manganese were synthesized using two different preparation methods, the polymeric precursor based on Pechini method and combustion reaction using a microwave. This was followed by impregnation with nickel content of 15 %. Samples were calcined at 300, 800 and 900 ?C and characterized by specific surface area (SSA), X-ray fluorescence (XRF), X-ray diffraction (XRD), scanning electron microscopy (SEM), temperature programmed reduction (TPR) and the reaction of partial oxidation of methane. The specific areas of samples decrease with the rise in calcination temperature and after nickel impregnation. Metal-cerium solid solution was formed and the presence of other manganese species outside the solid solution structure was confirmed in the compound with the highest amounts of manganese oxides showed. With regard to scanning electron microscopy, supports based on cerium and zirconium prepared by Pechini method exhibited agglomerated particles without uniform geometry or visible pores on the surface. However, compounds containing manganese presented empty spaces in its structure. Through synthesis by combustion reaction, morphology acquired independently of the proposed composition demonstrated greater porosity in relation to Pechini synthesis. Although catalysts were prepared using different synthesis methods, the insertion of nickel showed very similar reduction profiles (TPR). In relation to nickel catalysts supported on mixed oxide of cerium and zirconium, there is an initial reduction of NiO species that present certain interaction with the support. This is followed by the reduction of Ce4+ in Ce3+ surface, with subsequent bulk reduction. For catalysts containing manganese, a reduction of nickel oxide species occurs, followed by two stages of reduction for species Mn2O3 in Mn3O4 and Mn3O4 in MnO, with subsequent reduction of bulk. With respect to partial oxidation reactions, the nickel catalyst supported on mixed oxide of cerium and zirconium, prepared using the Pechini method, exhibited CH4 conversion of approximately 80 %, with conversion of 81 % when prepared by combustion. This behavior continued for 10 hours of reaction. Manganese content was also found to directly influence catalytic activity of materials; the greater the manganese oxide content, the faster deactivation and destabilization occurred in the catalyst. In both synthesis methods, the nickel catalyst supported on mixed oxide of cerium and zirconium maintained an H2/CO ratio very close to 2 during the 10 hours of partial oxidation reaction. Samples containing manganese displayed smaller H2/CO ratios and lower performance in partial oxidation. / Uma das principais aplica??es do metano ? a produ??o de g?s de s?ntese, mistura de hidrog?nio e mon?xido de carbono. Os processos utilizados na produ??o de g?s de s?ntese a partir do metano s?o: reforma a vapor, reforma com CO2, oxida??o parcial e reforma autot?rmica. Neste trabalho, o comportamento de catalisadores de n?quel suportados em ?xidos mistos de c?rio e mangan?s na rea??o de oxida??o parcial do metano foi avaliado e comparado com o catalisador de n?quel suportados no ?xido misto de c?rio e zirc?nio. Os ?xidos mistos de c?rio e zirc?nio ou c?rio e mangan?s foram sintetizadas usando dois diferentes m?todos de prepara??o; o de precursores polim?ricos baseado no processo Pechini e por rea??o de combust?o usando um micro-ondas, seguido da impregna??o de n?quel com teor de 15 %. As amostras foram calcinadas a 300, 800 e 900 ?C e caracterizados por ?rea espec?fica (ASE), fluoresc?ncia de raios X (FRX), difra??o de raios X (DRX), microscopia eletr?nica de varredura (MEV), redu??o ? temperatura programada (RTP) e a rea??o de oxida??o parcial do metano. As ?reas espec?ficas das amostras diminuem com o aumento da temperatura de calcina??o e ap?s a impregna??o com n?quel. A solu??o s?lida c?rio-metal foi formada e nos composto com as maiores quantidades de ?xidos de mangan?s verificou-se a presen?a de outras esp?cies de mangan?s fora da estrutura da solu??o s?lida. Quanto ? microscopia eletr?nica de varredura os suportes a base de c?rio e zirc?nio preparados via Pechini exibem part?culas aglomeradas, sem geometria uniforme e sem a visualiza??o de poros na superf?cie, enquanto os compostos contendo mangan?s apresentaram alguns vazios na sua estrutura. Atrav?s da s?ntese por rea??o de combust?o a morfologia adquirida independente da composi??o proposta apresentou uma maior porosidade em rela??o ? s?ntese Pechini. Mesmo sendo os catalisadores preparados por diferentes m?todos de s?ntese, a inser??o de n?quel deixou seus perfis de redu??o (RTP) muito semelhantes. Para os catalisadores de n?quel suportados no ?xido misto de c?rio e zirc?nio, h? em primeiro lugar redu??o de esp?cies NiO que apresentam certa intera??o com o suporte, seguido da redu??o de Ce4+ em Ce3+ superficiais, com posterior redu??o do bulk. Para os catalisadores contendo mangan?s h? a redu??o das esp?cies de ?xido de n?quel, seguido de duas etapas de redu??o para as esp?cies Mn2O3 em Mn3O4 e Mn3O4 em MnO, com posterior redu??o do bulk. Quanto ?s rea??es de oxida??o parcial, o catalisador de n?quel suportados no ?xido misto de c?rio e zirc?nio preparado via m?todo Pechini, apresentou uma convers?o de CH4 de cerca de 80 %, sendo 81 % a convers?o quando preparado via combust?o. Esse comportamento manteve-se durante 10 horas de rea??o. Observou-se tamb?m que o teor de mangan?s influencia diretamente na atividade catal?tica dos materiais, quanto maior o teor de ?xido de mangan?s mais r?pido o catalisador apresentava desativa??o e desestabiliza??o. Para ambos os m?todos de s?ntese o catalisador de n?quel suportados no ?xido misto de c?rio e zirc?nio manteve a raz?o H2/CO bem pr?xima de 2 durante as 10 horas em que ocorre a rea??o de oxida??o parcial. As amostras contendo mangan?s apresentaram menores raz?es de H2/CO e menor desempenho na oxida??o parcial.

Submitted by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-18T17:01:07Z No. of bitstreams: 2 carlos_felipe_reis_costa_guimaraes.pdf: 3938293 bytes, checksum: e0cdf597f1d9513ef2531c8a0cd6278f (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-18T17:56:25Z (GMT) No. of bitstreams: 2 carlos_felipe_reis_costa_guimaraes.pdf: 3938293 bytes, checksum: e0cdf597f1d9513ef2531c8a0cd6278f (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2014-12-18T18:01:44Z (GMT) No. of bitstreams: 2 carlos_felipe_reis_costa_guimaraes.pdf: 3938293 bytes, checksum: e0cdf597f1d9513ef2531c8a0cd6278f (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) / Made available in DSpace on 2014-12-18T18:01:44Z (GMT). No. of bitstreams: 2 carlos_felipe_reis_costa_guimaraes.pdf: 3938293 bytes, checksum: e0cdf597f1d9513ef2531c8a0cd6278f (MD5) license_rdf: 22974 bytes, checksum: 99c771d9f0b9c46790009b9874d49253 (MD5) Previous issue date: 2013 / Neste trabalho foi estudada a influ?ncia dos efeitos estruturais na rea??o de dimeriza??o associada ? obten??o do pept?deo antimicrobiano homotarsinina (Htr), bem como foram realizados ensaios hemol?ticos e de toxicidade desse pept?deo perante c?lulas humanas. Primeiramente foi realizada a s?ntese da cadeia monom?rica da homotarsinina (Htr-M), empregando-se a estrat?gia Fmoc de s?ntese em fase s?lida. Em seguida, o produto foi purificado por cromatografia l?quida de alta efici?ncia em fase reversa (CLAE-FR), tendo sido o homod?mero obtido sequenciado e caracterizado por espectrometria de massas (MALDI-ToF). Foram realizados estudos das prefer?ncias conformacionais da Htr-M por dicro?smo circular (CD) em diferentes meios e condi??es, como na presen?a de tamp?o Tris-HCl aquoso, em diferentes misturas de TFE-H2O e na presen?a de micelas de SDS. Realizou-se ent?o a rea??o de dimeriza??o do mon?mero em tr?s meios: (i) solu??o tamp?o Tris-HCl 100 mmol?L-1 pH 8,5, (ii) solu??o micelar de SDS 350 mmol?L-1 em tamp?o Tris-HCl 100 mmol?L-1 pH 8,5 e (iii) solu??o TFE:H2O 40:60 (v/v) em Tris-HCl 100 mmol?L-1 pH 8,5. O acompanhamento cin?tico das rea??es de dimeriza??o foi realizado por CLAE-FR. Como resultado, foi observado que em solu??o micelar de SDS a rea??o se completa em aproximadamente 6h (rendimento de 62%), enquanto que em solu??o tamp?o e em solu??o de TFE:H2O s?o necess?rias 24h e 48h (rendimentos de 66% e 85%), respectivamente. Os resultados de CD obtidos para a Htr-M em condi??es similares ?s empregadas nas rea??es de dimeriza??o mostraram que a Htr-M apresenta predominantemente conforma??o rand?mica em tamp?o Tris-HCl aquoso, enquanto adota predominantemente estrutura de h?lice ? em solu??o de TFE:H2O (40:60) ou na presen?a de micelas de SDS. Assim, os resultados do acompanhamento cin?tico da rea??o de dimeriza??o indicam que a conforma??o predominantemente rand?mica da Htr-M em tamp?o Tris-HCl aquoso, bem como a diminui??o de sua mobilidade e maior intera??o com a superf?cie das micelas de SDS e os efeitos de agrega??o que ocorrem em solu??o de TFE:H2O influenciam diretamente na forma de aproxima??o das cadeias monom?ricas, atuando de maneiras diferentes na forma??o da liga??o dissulfeto. Por fim, foram realizados ensaios de toxicidade em c?lulas humanas, os quais mostraram que Htr-M e Htr n?o apresentam toxicidade a leuc?citos mononucleares, al?m do que tamb?m n?o apresentam atividade hemol?tica. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2013. / ABSTRACT This dissertation is mainly related to studies concerning the role of the structural effects on the dimerization reaction that leads to the antimicrobial peptide homotarsinin (Htr). Besides, hemolytic assays as well as the toxicity of this peptide against human cells have also been investigated. Firstly, the monomeric chain (Htr-M) was obtained by Fmoc solid-phase synthesis. The product was purified by reverse phase high performance liquid chromatography (RP-HPLC) and then sequenced and characterized by mass spectrometry (MALDI-ToF). Circular dichroism (CD) studies regarding the Htr-M conformational preferences were performed for the peptide in different media and conditions, such as Tris-HCl aqueous buffer, TFE:H2O mixtures, as well as in the presence of SDS micelles. The dimerization reaction was then performed in three distinct media, namely: (i) Tris-HCl aqueous buffer 100 mmol?L-1 pH 8.5, (ii) SDS micellar solution of 350 mmol?L-1 in Tris-HCl 100 mmol?L-1 pH 8.5 and (iii) TFE:H2O 40:60 in Tris-HCl 100 mmol?L-1 pH 8.5. CD studies performed in similar conditions indicated that Htr-M presents predominantly random conformations in aqueous Tris-HCl buffer, whereas it adopts ?-helical arrangements in either TFE:H2O (40:60) solution or in the presence of SDS micelles. The kinetic monitoring of the dimerization reactions was carried out by RP-HPLC. The results indicated that the reaction is completed in about 6 h (yield 62%) in the presence of SDS micelles, whereas 24 h and 48 h (yields 66% and 85%) are necessary for the dimerizations performed in the aqueous Tris-HCl and in the TFE:H2O solutions, respectively. Therefore, the kinetic studies indicated that the predominantly random conformation of Htr-M in Tris-HCl aqueous buffer, as well as the mobility decrease alongside with the peptide-micelle interactions in the presence of SDS, as well as the aggregation effects in the TFE:H2O solution directly affect the inter-chain approach process, which leads to different pathways of the disulfide bond formation. Finally, toxicity assays were performed in human cells and the results showed that both Htr-M and Htr are not toxic against mononuclear leukocytes and also do not show hemolytic activity.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-17T22:37:29Z No. of bitstreams: 1 GabrielaLiberalinoLima_TESE.pdf: 6308180 bytes, checksum: b658a30fc2afc3a608b4b5b454c6d36a (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-02-19T22:18:54Z (GMT) No. of bitstreams: 1 GabrielaLiberalinoLima_TESE.pdf: 6308180 bytes, checksum: b658a30fc2afc3a608b4b5b454c6d36a (MD5) / Made available in DSpace on 2016-02-19T22:18:54Z (GMT). No. of bitstreams: 1 GabrielaLiberalinoLima_TESE.pdf: 6308180 bytes, checksum: b658a30fc2afc3a608b4b5b454c6d36a (MD5) Previous issue date: 2015-02-27 / O objetivo da presente tese foi utilizar a manipula??o de o?citos inclusos em fol?culos ovarianos pr? antrais (MOIFOPA) como ferramenta para o resgate e otimiza??o do uso de gametas femininos oriundos de esp?cies silvestres do bioma Caatinga. A tese foi dividida em quatro experimentos. No primeiro, foi realizada a estimativa e descri??o das caracter?sticas histol?gicas e ultraestruturas dos fol?culos pr? antrais (FOPA) de cutias (Dasyprocta leporina), nos quais foram estimados 4419.8 ? 532.26 e 5397.52 ? 574.91 fol?culos para os ov?rios direito e esquerdo, respectivamente, sendo a maioria (86,63%) pertencente a categoria de fol?culos primordiais (P<0,05). A maior parte da popula??o consiste de fol?culos morfologicamente normais (70,78%), apresentando n?cleo oocit?rio grande e central, com citoplasma uniforme. Na avalia??o ultraestrutural verificou-se a presen?a de um grande n?mero de mitoc?ndrias arredondadas e gotas de lip?dios. No segundo experimento, foi realizada a estimativa e a descri??o das caracter?sticas dos FOPA de pre?s (Galea spixii), bem como avalia??o do efeito da vitrifica??o em superf?cie s?lida (VSS) sobre a morfologia de FOPA in situ. O total de 416,0 ? 342,8 FOPA foi estimado por par de ov?rios e a presen?a de uma grande quantidade de fol?culos prim?rios foi evidenciada (P<0,05). A maior parte dos FOPA apresentou-se morfologicamente normal (94,6%), possuindo n?cleo oocit?rio contendo gr?nulos condensados de heterocromatina. Mitoc?ndrias com formato arredondado ou alongado representaram as organelas mais abundantes. Quanto a VSS, o protocolo utilizando o dimetilsulf?xido (DMSO) 3M possibilitou a preserva??o de 69,5% de FOPA morfologicamente normais, sendo evidenciado atrav?s da an?lise por microscopia de luz e eletr?nica de transmiss?o. No terceiro experimento, foi realizada a avalia??o do efeito da VSS sobre a morfologia e viabilidade de FOPA in situ de catetos (Pecari tajacu). N?o foram observadas diferen?as entre os tratamentos, onde o uso dos crioprotetores DMSO, etilenoglicol (EG) e dimetilformamida (DMF), independente da concentra??o utilizada (3 ou 6 M) promoveu a preserva??o da morfologia de mais de 70% dos FOPA. Quanto a viabilidade, os crioprotetores DMSO e EG, demonstraram melhor manuten??o da mesma. O quarto experimento teve por objetivo avaliar o efeito do ? MEM+ ou TCM199 associados ou n?o a 50 ng de FSHr sobre a morfologia, ativa??o e crescimento de FOPA de catetos, cultivados in vitro (CIV) durante 1 ou 7 dias e o efeito sobre a matriz extracelular (MEC). Ap?s 7 dias de CIV apenas o TCM199/FSH manteve a propor??o de FOPA intactos similar ao dia 1 (63,2%), contudo nenhuma diferen?a foi observada entre os tratamentos (P>0,05). Adicionalmente, um aumento na propor??o de FOPA em desenvolvimento foi verificada (P>0.05). Atrav?s da an?lise com Ag-NOR, observou-se que apenas o tratamento com TCM199/FSH manteve a propor??o de prolifera??o celular similar ao dia 1 (P>0.05). A colora??o com picrosirius red revelou que a MEC permaneceu intacta em todos os tratamentos (P>0.05). Assim, como conclus?o geral, o uso da MOIFOPA nas referidas esp?cies permitiu o conhecimento de aspectos relacionados a sua morfofisiologia reprodutiva, possibilitando tanto a conserva??o do material gen?tico destas esp?cies, com a possibilidade de forma??o de bancos de germoplasma, como a elucida??o de mecanismos relacionados a sobreviv?ncia e desenvolvimento dos FOPA in vitro. / The objective of the present thesis was to use the manipulation of oocytes enclosed in preantral follicles (MOEPF) as a tool for the female gametes rescue and optimization, from wild species of Caatinga biome. The thesis was divided into 4 experiments. At first experiment, it was performed the estimative and description of the agouti (Dasyprocta leporina) preantral follicles (PF) histologic and ultrastructural features, in which it was estimated 4419.8 ? 532.26 and 5397.52 ? 574.91 follicles for the right and left ovary, respectively, and the majority (86,63%) belonged to the primordial follicles category (P<0.05). Most of the population consists of morphologically normal follicles (70.78%), presenting a large and central nuclei and uniform cytoplasm. At ultrastructural evaluation it was verified the presence of a great number of round mitochondrias associated to lipid droplets. In the second experiment, it was performed the estimative and description of yellow-toothed cavies (Galea spixii) PF characteristics, also, the evaluation of the effect of solid surface vitrification (SSV) on the in situ PF morphology. The total of 416.0 ? 342.8 PF was estimated for the ovary pair and the presence of a large quantity of primary follicles (P<0.05) was evidenced. Most of the PF was morphologically normal (94.6%), in which the oocyte nuclei presented condensed granules of heterochromatin. Round or elongated shaped mitochondria constituted the most abundant organelles. In regard of the SSV, the protocol using the dimethylsulfoxide (DMSO) 3M possibility the preservation of 69.5% of morphologically normal PF, which was evidenced by the light and transmission electronic microscopy. At third experiment, the evaluation of the SSV procedure on the morphology and viability in situ PF form collared peccaries (Pecari tajacu) was performed. No differences were observed among treatments, in which the use of DMSO, ethylene glycol (EG) and dimethylformamide (DMF) as cryoprotectants, regardless its concentration, promoted the morphology preservation of much than 70% of PF. Concerning the PF viability, the DMSO and EG promoted the best preservation. The fourth experiment aimed to evaluate the effect of ? MEM+ or TCM199 associated or not to 50 ng of FSHr on the morphology, activation and growth of collared peccaries PF, in vitro cultured (IVC) during 1 or 7 days and the effect on the extracellular matrix (ECM). After 7 days of IVC only the use of TCM199/FSH maintained the proportion of intact PF, similar to day 1(63.2%), however, no differences were observed among treatments (P>0.05). Also, an improvement of the proportion of intact growing PF was verified (P>0.05). By the Ag-NOR analysis it was observed that only the treatment using TCM199/FSH promoted the maintenance of cell proliferation similar to day 1 (P>0.05). The picrosirius red stain revealed that ECM remained intact in all treatments (P>0.05). Thus, as the general conclusion, the use of MOEPF in the refereed species allowed the knowledge of aspects related to its reproductive morphology and physiology, enabling the germplasm conservation, with the possibility of germplasm bank formation, as the elucidation of mechanisms related to the PF survive and in vitro development.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-09T23:41:38Z No. of bitstreams: 1 FranciscoFrancimarDaFonsecaSilva_DISSERT.pdf: 3949069 bytes, checksum: ef5faf7977215a513bc7ccfea51becf9 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-14T19:35:38Z (GMT) No. of bitstreams: 1 FranciscoFrancimarDaFonsecaSilva_DISSERT.pdf: 3949069 bytes, checksum: ef5faf7977215a513bc7ccfea51becf9 (MD5) / Made available in DSpace on 2016-03-14T19:35:38Z (GMT). No. of bitstreams: 1 FranciscoFrancimarDaFonsecaSilva_DISSERT.pdf: 3949069 bytes, checksum: ef5faf7977215a513bc7ccfea51becf9 (MD5) Previous issue date: 2015-02-27 / A detec??o de microcontaminantes de interesse emergente em amostras ambientais de ?guas superficiais, ?guas subterr?neas, ?gua pot?vel, ?guas residu?rias e efluentes de esta??es de tratamento de ?gua e esgoto (ETAs e ETEs), em v?rios pa?ses, indica que esses poluentes encontram-se disseminados pelo meio ambiente, principalmente em regi?es urbanizadas. Isto ? motivo de grande preocupa??o, pois muitos desses compostos s?o potencialmente prejudiciais aos seres humanos e a outros seres vivos e n?o s?o removidos eficientemente na maioria das ETAs e ETEs, o que ? agravado pela precariedade dos servi?os de abastecimento de ?gua e esgotamento sanit?rio. Em Natal/RN, a exemplo de outras cidades brasileiras, o sistema de esgotamento sanit?rio atende apenas uma parte da ?rea urbana (cerca de 30%), de modo que o restante do esgoto ? infiltrado no solo arenoso da regi?o, em sistema de fossa s?ptica e sumidouro. Isto tem resultado na contamina??o das ?guas subterr?neas da regi?o (aqu?fero Dunas-Barreiras, que abastece mais de 50% da popula??o da cidade), o que tem sido observado pela eleva??o da concentra??o de nitrato nos po?os de abastecimento. A vulnerabilidade do aqu?fero DunasBarreiras, aliada aos relatos da presen?a de microcontaminantes de interesse emergente no Brasil e no mundo, motivaram a realiza??o desta pesquisa, que investigou a ocorr?ncia de quinze microcontaminantes nas ?guas subterr?neas e nos esgotos de Natal/RN. Foram coletadas amostras em cinco po?os usados para abastecimento, no esgoto bruto e nos efluentes dos reatores biol?gicos de uma ETE (UASB seguido de sistema de lodo ativado). Foram realizadas duas coletas de cada amostra, com uma semana de intervalo entre as coletas. Para a determina??o dos contaminantes, foi realizada a extra??o das amostras de ?gua do aqu?fero, e do esgoto bruto e tratado atrav?s da t?cnica SPE utilizando cartuchos Strata X (Phenomenex?), para a ?gua do aqu?fero, e Strata SAX e Strata X (Phenomenex?), para as amostras de esgotos bruto e tratado. Posteriormente os extratos foram analisados utilizando a t?cnica de CG-MS. Grande parte dos microcontaminantes analisados foram detectados nas ?guas subterr?neas e nos esgotos, sendo as concentra??es nas ?guas subterr?neas geralmente inferiores ?s dos esgotos. Alguns dos microcontaminantes (estrona, estradiol, bisfenol A, cafe?na, diclofenaco, naproxeno, paracetamol e ibuprofeno) s?o parcialmente removidos na ETE. / The detection of emerging interest microcontaminants in environmental samples of surface water, groundwater, drinking water, wastewater and effluents from water and sewage treatment plants (WTP and STP), in many countries, suggests these pollutants are widespread in the environment, mainly in urban areas. This is a reason for great concern, since many of these compounds are potentially harmful for humans other living beings, and they are not efficiently removed in the majority of WTP and STP, which is exacerbated by precariousness of water supply and sanitation services. In Natal, like other Brazilian cities, the sewage system serves only part of the urban area (about 30%), so that the rest of the wastewater is infiltrated in the sandy soil of the region in cesspool-dry well systems. This has resulted in contamination of groundwater in the area (sand-dune barrier aquifer, which supplies more than 50% of the city population), which has been observed by the increase in nitrate concentration in supply wells. The vulnerability of the sanddune barrier aquifer, combined with reports of the presence of emerging interest microcontaminants in Brazil and worldwide, led to this research, which investigated the occurrence of fifteen microcontaminants in Natal groundwater and sewage. Samples were collected at five wells used for water supply, the raw sewage and the effluents from biological reactors from STP (UASB and activated sludge reactors). Two samples of each sample were taken, with one week apart between the samples. To determine the contaminants, extraction of aquifer water, and raw and treated sewage samples were performed, through the technique of using SPE Strata X cartridge (Phenomenex?) to the aquifer water, and Strata SAX and Strata X (Phenomenex? ) for samples of raw and treated sewage. Subsequently the extracts were analyzed using GC-MS technique. Much of the analyzed microcontaminants were detected in groundwater and sewage. The concentrations in groundwater are generally lower than those found in the sewers. Some of the compounds (estrone, estradiol, bisphenol A, caffeine, diclofenac, naproxen, paracetamol and ibuprofen) are partially removed at STP.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-06T12:10:14Z No. of bitstreams: 1 2017 - Paulo Pitasse Santos.pdf: 13320307 bytes, checksum: b714828ac2a5d9a1d2ab188470e04e9a (MD5) / Made available in DSpace on 2017-09-06T12:10:14Z (GMT). No. of bitstreams: 1 2017 - Paulo Pitasse Santos.pdf: 13320307 bytes, checksum: b714828ac2a5d9a1d2ab188470e04e9a (MD5) Previous issue date: 2017-02-20 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Chagas disease was studied and described by the Brazilian sanitarist and physician Carlos Chagas in 1909. It is caused by the protozoan Trypanosoma cruzi and presents complex clinical manifestations. However, since its discovery, little progress has been made in the chemotherapeutic treatment of Chagas' disease. The only available drug for its treatment (benzonidazole) is not completely efficient and is associated with the development of several side effects. From the knowledge of the antiparasitic activity of the natural amidic alkaloid piperine, this work focused on the proposition of new structurally-similar molecules with trypanocidal potential. From the principles of bioisosterism, a series of new 1,2,4-oxadiazoles were proposed. Its synthesis was designed from the corresponding 3-arylacrylic acids to give the respective acyl chlorides by reaction with oxalyl chloride. The subsequent step involves O-acylation of the properly substituted benzamidoxime following the cyclization reaction of the oxadiazolic ring, which occurs in solid support (silica gel) using microwave irradiation. The characterization of the products was done by determination of melting points, 1H and 13C NMR, infrared espectrometry and high and low resolution mass spectrometry. The present work also presents information about the biological activity profile of the molecules synthesized against epimastigote forms of the T. cruzi protozoan and against primary mammalian cells, allowing the calculation of their selectivity indexes. Investigations about the possible mechanisms of action of the derivatives on T. cruzi indicate that there are no influences on the enzymatic action of the protease cruazain, on the cell cycle of the parasite or on the biosynthesis of membrane sterols catalyzed by the enzyme CYP51. The developed sinthetic methodology can be applied in the expansion of the series of analogues derivatives. The perspectives of this work also include the biological evaluation against amastigote and trypomastigote forms of the parasite. / A doen?a de Chagas foi estudada e descrita pelo m?dico sanitarista e cientista brasileiro Carlos Chagas, em 1909. ? causada pelo protozo?rio Trypanossoma cruzi, apresentando manifesta??es cl?nicas complexas. No entanto, desde sua descoberta, pouco se avan?ou no tratamento quimioter?pico da doen?a de Chagas, sendo o f?rmaco dispon?vel (benzonidazol) pouco eficiente e associado ? manifesta??o de diversos efeitos colaterais. A partir do conhecimento da atividade antiparasit?ria da amida natural piperina, este trabalho focou-se na proposi??o de novas mol?culas estruturalmente semelhantes com potencial tripanocida. A partir dos princ?pios do bioisosterismo, foi proposta uma s?rie de novos 1,2,4-oxadiaz?is diferentemente substitu?dos. Sua s?ntese foi concebida partir dos ?cidos 3-arilacr?licos correspondentes, obtendo-se os respectivos cloretos de acila, atrav?s da rea??o com cloreto de oxalila. A etapa posterior envolve a O-acila??o da benzamidoxima adequadamente substitu?da, seguida do fechamento do anel oxadiaz?lico, que se d? em em suporte s?lido (s?lica-gel) empregando-se irradia??o de micro-ondas. A caracteriza??o dos produtos foi feita atrav?s de ponto de fus?o, RMN 1H e 13C, espectrometria no infravermelho e espectrometria de massas de alta e baixa resolu??o. O presente trabalho ainda traz informa??es quanto ao perfil de atividade biol?gica das mol?culas sintetizadas frente a formas epimastigotas do protozo?rio Trypanosoma cruzi e frente a c?lulas prim?rias de mam?feros, permitindo que se calculasse o seu ?ndice de seletividade. Investiga??es quanto a poss?veis mecanismos de a??o dos derivados sobre o T. cruzi indicam n?o haver influ?ncias sobre a a??o enzim?tica da protease cruza?na, sobre o ciclo celular do parasito, nem sobre a bioss?ntese de ester?is de membrana, catalisada pela enzima CYP51. A metodologia qu?mica desenvolvida poder? ser aplicada na s?ntese de outros an?logos. As perspectivas deste trabalho incluem ainda a avalia??o biol?gica frente a formas amastigota e tripomastigota do parasito

Made available in DSpace on 2014-12-17T14:06:48Z (GMT). No. of bitstreams: 1 WersonMC.pdf: 1184207 bytes, checksum: f2f0598e463a68730749344c713c5bff (MD5) Previous issue date: 2008-02-22 / The Tungsten/copper composites are commonly used for electrical and thermal objectives like heat sinks and lectrical conductors, propitiating an excellent thermal and electrical conductivity. These properties are dependents of the composition, crystallite size and production process. The high energy milling of the powder of W-Cu produces an dispersion high and homogenization levels with crystallite size of W very small in the ductile Cu phase. This work discusses the effect of the HEM in preparation of the W-25Cu composite powders. Three techniques of powder preparation were utilized: milling the dry with powder of thick Cu, milling the dry with powder of fine Cu and milling the wet with powder of thick Cu. The form, size and composition of the particles of the powders milled were observed by scanning electron microscopy (SEM). The X-ray diffraction (XRD) was used to analyse the phases, lattice parameters, size and microstrain of the crystallite. The analyse of the crystalline structure of the W-25Cu powders milled made by Rietveld Method suggests the partial solid solubility of the constituent elements of the Cu in lattice of the W. This analyse shows too that the HEM produces the reduction high on the crystallite size and the increase in the lattice strain of both phases, this is more intense in the phase W / Os comp?sitos de Tungst?nio/Cobre (W-Cu) s?o geralmente usadas para fins el?tricos e t?rmicos como dissipadores de calor e condutores el?tricos, devido as suas excelentes propriedades de condutividades t?rmica e el?trica. Essas propriedades s?o dependentes da composi??o, do tamanho de cristalito e principalmente do processo de fabrica??o. A moagem de alta energia de p?s W-Cu produz alto n?vel de homogeneiza??o e dispers?o com cristalitos de W muito fino na fase d?ctil Cu. Este trabalho discute o efeito da MAE na prepara??o dos p?s comp?sitos W-25Cu. Tr?s t?cnicas de prepara??o dos p?s foram utilizadas: moagem a seco com p? de Cu grosso, moagem a seco com p? de Cu fino e moagem a ?mido com p? de Cu grosso. A forma, tamanho e a composi??o das part?culas dos p?s mo?dos foram observadas por microsc?pio eletr?nico de varredura (MEV). A difra??o de raios-X(DRX) foi usada para observar as fases, par?metros de rede, tamanho e microtens?o dos cristalitos. A an?lise da estrutura cristalina dos p?s mo?dos de W-25Cu feita pelo m?todo de Rietveld sugere uma solubilidade s?lida parcial dos elementos constituintes do cobre (Cu) na rede do tungst?nio (W). Essa an?lise tamb?m mostra que a MAE produz uma alta redu??o no tamanho dos cristalitos e um aumento de tens?o na rede de ambas as fases, isto ocorre com maior intensidade na fase do W

?rea de concentra??o: Ci?ncias farmac?uticas. / Data de aprova??o ausente. / Disponibiliza??o do conte?do parcial, conforme Termo de Autoriza??o no trabalho. / Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2016-12-20T19:20:11Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-01-17T18:55:36Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5) / Made available in DSpace on 2017-01-17T18:55:36Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) sara_pacelli_sousa_marcial_parcial.pdf: 166236 bytes, checksum: c01c4f087337cd82c64b06b90a1cc4dc (MD5) Previous issue date: 2016 / Funda??o de Amparo ? Pesquisa do Estado de Minas Gerais (FAPEMIG) / O paclitaxel (PTX) ? um agente quimioter?pico que tem uma importante fun??o no tratamento de v?rios tipos de c?ncer, especialmente o c?ncer de mama. No entanto, a baixa solubilidade do PTX em meio aquoso (coeficiente de parti??o log = 3,96) representa uma limita??o para a administra??o intravenosa. A formula??o convencional do PTX cont?m uma alta concentra??o de Cremofor-EL? (derivado polietoxilado do ?leo de r?cino), o qual induz significante toxicidade, restringindo sua utiliza??o cl?nica. A encapsula??o do PTX em sistema de libera??o de f?rmacos pode melhorar a absor??o e aumentar a sua efic?cia terap?utica. Neste estudo, tr?s diferentes nanossistemas lip?dicos contendo PTX, nanopart?culas lip?dicas s?lidas (NLS), nanoemuls?o (NE) e carreadores lip?dicos nanoestruturados (CLN) foram preparados e as propriedades f?sico-qu?micas e a atividade citotoxicidade in vitro foram avaliadas. Em rela??o ao di?metro m?dio, o CLN branco mostrou valor de di?metro aproximadamente 2 e 1,7 vezes menor que os obtidos para NLS e NE, respectivamente. A presen?a de PTX levou a um aumento significativo no di?metro das part?culas em todos os sistemas avaliados, exceto no NE. Al?m disso, o aumento da concentra??o do f?rmaco (0,01% para 0,025%) produziu um aumento do di?metro para a prepara??o de CLN. Todas as formula??es com PTX mostraram ?ndice de polidispers?o superior a 0,3, exceto para NE-PTX na concentra??o do f?rmaco igual a 0,01% (p/v). Valores negativos de potencial zeta foram observados para todas as formula??es avaliadas. CLN-PTX foi o sistema mais est?vel ap?s armazenado por 30 dias a 4 ?C. O estudo de citotoxicidade nas linhagens celulares de c?ncer de mama (MDA-MB-231 e MCF-7) demonstrou atividade citot?xica mais pronunciada para CLN-PTX do que para o PTX livre em ambos as linhagens celulares do tumor. Baseado nesses resultados, CLN-PTX parece ser uma ferramenta potencial para o tratamento do c?ncer de mama. / Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Ci?ncias Farmac?uticas, Universidade Federal dos Vales do Jequitinhonha e Mucuri, [2016]. / Paclitaxel (PTX) is a chemotherapeutic agent that plays an important role in the treatment of several types of human cancer, especially breast cancer. However, the low solubility of PTX in aqueous medium (partition coefficient log of 3.96) represents a barrier for intravenous administration. The conventional PTX formulation contains a high concentration of Cremophor-EL? (polyethoxylated castor oil), which is associated with significant toxicities restricting its clinical use. The encapsulation of the PTX in drug delivery systems could improve the uptake and increase its therapeutic efficacy. In this study, three different lipid nanosystems containing PTX, solid lipid nanoparticle (SLN), nanoemulsion (NE), and nanostructured lipid carrier (NLC) were prepared, and the physicochemical properties and in vitro cytotoxic activity were evaluated. Concerning the mean diameter, NLC blank showed diameter values approximately 2 and 1.7-fold lower than those obtained for SLN and NE, respectively. The presence of PTX leads to a significant increase in the particle diameter in all systems evaluated, except NE. In addition, increases in drug concentration (0.01% to 0.025%) produced an enhanced diameter for NLC preparation. All formulations containing PTX showed PI higher than 0.3, except for NE-PTX at drug concentration equal to 0.01% (w/v). Negative zeta potential values were observed in all formulations evaluated. NLC-PTX was the system more stable after storage for 30 days at 4 oC. The cytotoxicity studies on breast cancer cell lines (MDA-MB-231 and MCF-7) demonstrated cytotoxic activity more pronounced for NCL-PTX than for free PTX for both tumor cell lines. Thus, the results showed that NCL-PTX seems to be a potential tool for the treatment of breast cancer.

This dissertation examines lesbian and queer desire in texts in Catalan and Spanish written in Barcelona, Montevideo, and Buenos Aires from the 1960s to the present. In the texts, desire includes but is not limited to the erotic; it encompasses issues of queer textuality, relationality, and literary transmission. I focus on the works of three authors who have spent the majority of their lives in Barcelona. However, the city appears almost incidentally in their works; the genealogies that the authors trace are transnational. The texts combine literal movement (through exile or diaspora) and a metaphorical sense of being “out of place” that prompts writers to take refuge in writing. I demonstrate that despite depicting affinities beyond the family and nation, the works reveal the persistence of familial and national ties, albeit in spectral or queer ways. Rather than tracing continuous lines of descent that emphasise origins, the works are principally concerned with futurity and fragmentation, as in Michel Foucault’s reading of genealogy. Chapter One on Maria-Mercè Marçal’s La passió segons Renée Vivien (1994) traces a literary genealogy from Sappho to Renée Vivien in fin de siècle Paris to Marçal. The novel represents a merging of literary desire and erotic desire; Marçal’s search for symbolic mothers turns out to be a search for symbolic lovers that is oriented towards the present and future. In Chapter Two, I posit that in Cristina Peri Rossi’s La nave de los locos (1984) “happiness” consists of being open to chance and unpredictability unlike in conventional “happy” scripts in which a valuable life is believed to consist of (heterosexual) marriage, children, and property ownership. In Part II I argue that through fragmentation, allegory, and ambiguity, Peri Rossi’s El libro de mis primos (1969) contests authoritarian discourse without itself becoming a site of hegemonic meaning. In inviting the reader’s collaboration, it ensures authorial legacy. Part I of Chapter Three is an analysis of the temporality of obsession in Flavia Company’s Querida Nélida (1988). I propose that obsession and melancholia may point to a utopian future rather than signalling an entrapment in the past. My study of Melalcor (2000) in Part II suggests that queer forms of relationality that are not centred on procreation and monogamy offer ethical models of sociality. Part III focusses on Company’s return to biological family in Volver antes que ir (2012) and Por mis muertos (2014). The resurgence in these texts of family members who have died signals that just as the queer haunts the family, the family haunts the queer.