Le projet de thèse consistait à étudier le mouvement du Virus de la jaunisse du navet (TuYV) dans le système vasculaire. Le premier objectif était d’identifier la nature du complexe viral cheminant dans les tubes criblés : virions et/ou complexes ribonucléoprotéiques. L’analyse du mouvement de mutants viraux dans différentes espèces végétales, en absence ou en présence de protéines de capside de type sauvage apportées en trans, a permis de démontrer une étroite relation entre la formation de virions et le transport à longue distance. Le second objectif de cette étude portait sur l’identification de partenaires cellulaires de la protéine P4 du TuYV. Deux protéines ont été identifiées par un criblage de banques d’ADNc d’A. thaliana par le système du double hybride dans la levure, et l’analyse de leur implication dans le cycle viral a été amorcée par des expériences de localisation subcellulaire et de validation fonctionnelle in planta. / In the project, Turnip yellows virus (TuYV) transport in the phloem was analysed. The first objective was to identify the nature of the viral complex involved in vascular movement: virions and/or ribonucleoprotein complexes. Mutant viruses were modified in the capsid protein gene to inhibit formation of virions. By analyzing their movement in different host plants, in the absence or in the presence of the wild-type capsid proteins brought in trans, we demonstrated a strong relation between virion formation and virus long-distance movement. The second objective was to identify cellular partners of the TuYV-P4 protein, a putative movement protein which is host-specific. Two proteins were identified by screening a cDNA library of A. thaliana using the yeast two hybrid technique, and their function in the virus cycle was assessed by performing sub-cellular localizations and infection of A. thaliana KO mutants.

Este trabalho tem como objetivo principal direcionar a tomada de decisões para a escolha dos diferentes parâmetros que envolvem o alinhamento horizontal e vertical do projeto geométrico ferroviário de trens de alta velocidade (TAV) e de longo percurso, bem como descrever conceitos teóricos para parâmetros de segurança e conforto, baseados em normas internacionais. A pesquisa se desenvolve através de consultas em revistas especializadas, artigos, publicações, normas técnicas e livros. Pela escassez de dados em língua nacional e material bibliográfico desatualizado, as pesquisas se concentram essencialmente em material internacional. Apesar do Brasil ser um dos países em desenvolvimento com maior ascensão no cenário global, tem apresentado, nas últimas décadas, sérios problemas de infra-estrutura devido a uma distribuição desequilibrada na área de transportes, com altíssima concentração no modo rodoviário, com aeroportos e rodovias operando acima da capacidade satisfatória, além de um leque limitado de alternativas de transporte. Devido à falta de investimentos no setor ferroviário, grande parte da malha ferroviária brasileira tornou-se inoperante ou até mesmo inexistente, resultando numa enorme diminuição da mão de obra qualificada e formação acadêmica nesse ramo. Como conseqüência, os estudos relacionados ao projeto ferroviário também foram afetados diretamente. Nesse contexto, é importante que se resgate normas, publicações, especificações técnicas e experiências adotadas em outros países com sólidas tradições ferroviárias, de modo a incorporar em nossa formação acadêmica tais conceitos, como é um dos objetivos dessa dissertação. / This dissertation has as main objective to guide in taking decisions for the selection of different parameters which involve the horizontal and vertical railway geometric design of high speed (HST) and long distance trains as well as theoretical concepts for describing security parameters and comfort, based on international standards. The research is developed through consultations in specialized magazines, articles, publications, standards and technical books. Due to the scarcity of data in the national language and outdated library materials, the research focuses primarily on international material. Although Brazil is a developing country with one of the highest rises in the global scene, the country has faced in recent decades serious infrastructure problems due to an uneven distribution in the area of transport, with high concentration in the road, and airports and roads operating above satisfactory capacity, resulted by a limited range of transportation alternatives. Due to the lack of investment in the railway sector, much of the Brazilian railway system became inoperative or even nonexistent, resulting in a tremendous decrease of skilled labor and academic training in this field. As a result, studies related to the railway project have also been directly impacted. In this context, it is important to rescue standards, publications, experiences and technical specifications adopted in other countries with strong tradition in railways in order to incorporate these concepts into our academic formation, which is one of the purposes of this dissertation.

Submitted by Renata Lopes (renatasil82@gmail.com) on 2016-06-02T15:48:39Z No. of bitstreams: 1 veronicadecastrolameira.pdf: 1250198 bytes, checksum: af0c0263e3fc8188a4d53c8627567747 (MD5) / Approved for entry into archive by Adriana Oliveira (adriana.oliveira@ufjf.edu.br) on 2016-07-02T13:16:07Z (GMT) No. of bitstreams: 1 veronicadecastrolameira.pdf: 1250198 bytes, checksum: af0c0263e3fc8188a4d53c8627567747 (MD5) / Made available in DSpace on 2016-07-02T13:16:07Z (GMT). No. of bitstreams: 1 veronicadecastrolameira.pdf: 1250198 bytes, checksum: af0c0263e3fc8188a4d53c8627567747 (MD5) Previous issue date: 2012-12-20 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / A análise da mobilidade de trabalhadores exerce papel fundamental na tomada de decisões em políticas públicas. O primeiro ensaio aborda a questão da mobilidade de curta e longa distância, e ainda os fatores regionais da atração de trabalhadores entre as microrregiões do Brasil, utilizando modelo binomial negativo. Houve a constatação da preferência dos trabalhadores por destinos com maior atratividade relativa, admissão de trabalhadores frente aos desligados, densidade, proporção de graduados, menor criminalidade e congestionamento. Para o modelo de longa distância os fluxos são orientados para destinos com menor densidade populacional, menor grau de industrialização, menor criminalidade e maior atração relativa. O congestionamento se mostrou irrelevante na mobilidade de longa distância. A distância figura como fator inibidor para a mobilidade, independente dos cortes de distância. O segundo ensaio adota o modelo logit multinível espacial com o objetivo de identificar os condicionantes individuais e regionais da mobilidade de trabalhadores qualificados nas microrregiões brasileiras. Foi possível verificar que a experiência do trabalhador figura como fator inibidor da mobilidade, a expectativa salarial reflete menor propensão à mudança para trabalhadores em geral, e maior probabilidade para qualificados. Algumas variáveis de contexto também se mostram importantes para explicar a mobilidade, como PIB per capita, proporção de trabalhadores com ensino superior completo, taxa de homicídio, veículos por habitantes, grau de industrialização e rede de migrantes. / The analysis of labor mobility plays a fundamental role in decision-making in public policy. The first essay addresses the issue of mobility by long and short distance, and the regional factors of attraction for workers among Brazilian microregions, by means of negative binomial model. There was the realization of the preferred destinations for workers with higher relative attractiveness, employment of workers off against, density, proportion of graduates, less crime and congestion. To model long-distance flows are oriented destinations with lower population density, lower degree of industrialization, less crime and greater relative attractiveness. Congestion proved irrelevant in the long-distance mobility. The distance appears as an inhibiting factor for mobility, regardless of the cuts away. The second essay adopts a spatial multilevel logit model in order to identify the determinants of individual and regional mobility of skilled workers in the Brazilian microregions. The results indicate that the worker seniority figures as inhibitory factor of mobility, and expected wage decrease the propensity of mobility for workers in general, though it increases the propensity of the more skilled ones. The context variables also show important to explain mobility, such as GDP per capita, proportion of workers with higher education, rate of homicide by vehicle inhabitants, degree of industrialization and net migrants.

Ce manuscrit de thèse s’intéresse à l’étude théorique et à l’observation d’effets quantiques résultantde la manipulation de photons en fréquence. Ainsi nous rapportons une expérience d’interférenceà deux photons, pour laquelle l’intrication et la manipulation des photons survient dans le domainedes fréquences. Nous montrons que cette figure d’interférence est sensible à la dispersion des deuxphotons jumeaux et que ce phénomène peut être compensé de manière non-locale. D’un autre coté,nous étudions la réalisation d’une marche aléatoire quantique établie par la modulation de phase.Nous mettons en évidence un comportement intéressant de ces marches et suggérons un schémaexpérimental. / This manuscript deals with a theoretical and experimental study of quantum effects taking place inthe frequency domain. On one side, we report a two photons interference experiment, where both theentanglement of the photons and their manipulation take place in the frequency domain. We showthat this interference pattern is sensitive to the dispersion of both photons and allows us to perform anonlocal dispersion cancellation. On the other side we study the implementation of a quantum walkbased on the phase modulation. We predict an interesting behavior of these quantum walks andsuggest a physical implementation.

Bien que constitué anatomiquement et physiologiquement pour la course d’endurance, l’Homme est considérablement exposés à diverses blessures musculo-squelettiques liées à la répétition de contraintes mécaniques. Le coureur de trail running par exemple est soumis à de nombreux impacts ainsi qu’à une fatigue et des dommages musculaires sévères. Ces chocs répétitifs et dommages musculaires réduiraient la tolérance du coureur face aux contraintes mécaniques le poussant ainsi à altérer sa cinématique de course. Par conséquent, minimiser les dommages musculo-squelettiques serait déterminant pour la performance en trail running. Des évidences montrent que la pose de pied altère la localisation et l’intensité des contraintes appliquées au système musculo-squelettique. L’objectif de ce travail de thèse a été d’étudier l’influence du pattern de pose de pied sur l’impact et la fatigue neuromusculaire en trail running. Les phases de descente ont été tout particulièrement étudiées du fait qu’elles sont les plus traumatisantes. En effet, ce travail de thèse a mis en évidence qu’en situation de trail running, l’intensité de l’impact augmente lorsque la pente diminue, et que la fatigue neuromusculaire périphérique est aussi sévère à la suite d’une descente isolée qu’après un ultra-trail de plusieurs heures. En étudiant l’influence de la pose de pied adoptée au cours d’une descente en situation de trail running, il a été observé qu’attaquer le sol par l’avant du pied augmentait la fatigue neuromusculaire aux extenseurs du genou. Cependant, une importante variabilité dans les patterns de pose de pied adoptés au cours de la descente a été associée à une baisse de la fatigue neuromusculaire aux extenseurs du genou et fléchisseurs plantaires. L’influence de la pose de pied sur l’intensité du choc et le contenu vibratoire le long des axes axial et transversal a également été démontrée : adopter une attaque talon diminue la sévérité du choc axial mais réduit l’intensité du choc transversal. La principale conclusion est qu’aucun pattern de course ne saurait être universellement recommandé du fait que « changer de pose de pied » est synonyme de « changer la localisation et la magnitude des contraintes appliquées au système musculo-squelettique ». En ce sens, alterner entre différents patterns de course serait une stratégie efficiente en trail running / Although Humans are “born” anatomically and physiologically adapted to long distances run, they are substantially exposed to various musculoskeletal overuse injuries. Trail runners sustain a high number of foot-to-ground contacts and develop severe muscle fatigue and damages. Repetitive shocks and muscle damages would reduce the runners’ tolerance to mechanical strains leading to changes in running kinematics. Minimizing musculoskeletal damages is therefore considered paramount for performance in trail running. Numerous studies highlighted that the foot strike pattern alters the localization and magnitude of the mechanical strains applied on the musculoskeletal system. The main purpose of this thesis was to study the influence of the foot strike pattern on impact and neuromuscular fatigue in trail running. Downhill sections were mainly investigated since they are the most mechanically stressful. Indeed, it was observed from this thesis’ work that, in real trail running practice, the impact intensity increases as the slope decreases, and that the neuromuscular fatigue induced by a single downhill run is as severe as the one induced by an ultratrail race that lasts several hours. Investigating the effect of the foot strike pattern adopted during a downhill trail run on fatigue, it was observed that forefoot striking increases the neuromuscular fatigue at knee extensors. However, a high variability in foot strike patterns adopted was associated to a lower neuromuscular fatigue at both knee extensors and plantar flexors. The effect of the foot strike pattern on axial and transversal shock and vibration content was also demonstrated: heel striking was correlated to a lower impact severity along the axial axis of the skeleton but a greater one along its transversal axis. The main conclusion of this thesis is that no single foot strike pattern should be universally advised due to “changing of foot strike” means “changing the localization and magnitude of the mechanical stress applied on the musculoskeletal system”. Switching between different running patterns might be an efficient strategy in trail running

[pt] O objetivo deste estudo foi determinar a concentração de metais, compostos orgânicos e inorgânicos por suspensão aquosa e análise dos metais por abertura ácida presentes nas amostras de PM10 coletadas em áreas urbanas, industrial e rural da Região Metropolitana do Rio de Janeiro. A concentração de PM10 foi determinada por análise gravimétrica. Espécies inorgânicas e orgânicas presentes nos extratos aquosos foram determinadas por cromatografia de íons e carbono orgânico total (TOC). Os extratos ácidos, e também os aquosos, foram analisados por ICP-MS (espectrometria de massa com plasma indutivamente acoplado) e por ICP OES (espectrometria de emissão óptica com plasma indutivamente acoplado) para determinar a concentração de metais. As correlações entre as concentrações de PM10 e de metais foram avaliadas, considerando os dados meteorológicos para cada ponto de coleta e origem das massas de ar. Os resultados mostraram que as concentrações médias de PM10 foram de 36 micrograma m-3 na área rural, 50 micrograma m-3 na área urbana e 74 micrograma m-3 na área industrial. A concentração de PM10 medida na área industrial ultrapassou, em geral, o limite estabelecido pela resolução CONAMA, de 50 micrograma m-3. O teor de metais apresentou maior concentração na área industrial, sendo predominantes ferro, zinco, alumínio, titânio, manganês, cromo, níquel, cádmio e chumbo. Na área de maior tráfego, foi detectada a maior concentração de cobre e vanádio. As espécies iônicas foram maiores na área urbana, devido à proximidade do oceano. Na área rural, menores concentrações de espécies antropogênicas, como sulfato, nitrato e alguns metais (Pb, Cr, V e Ni) foram medidas. Assim, as concentrações das espécies estudadas são influenciadas pelas características locais. Porém, também foi observado a influencia do transporte de poluentes de longa distância, como queimadas e poeiras dos desertos do continente africano. Os percentuais dos extratos ácido/aquoso demonstraram que os metais extraídos de forma mais eficiente foram V e Ni (maior que 45 porcento), enquanto que os menos extraídos foram Al e Fe (menor que 3 porcento), conforme o esperado. A fração aquosa é importante para avaliar a biodisponibilidade de metais associados a danos à saúde. / [en] The scope of this study was to measure the concentration of metals and organic and inorganic anions in aqueous and acid extracts of PM10 samples collected in urban, industrial and rural zones of Rio de Janeiro. PM10 concentration was determined by gravimetric analysis and the organic and inorganic species in the aqueous extracts were determined by ion chromatography and total organic carbon (TOC). The aqueous and acid extracts were analyzed by inductively coupled plasma mass spectrometry (ICP-MS) and by ICP OES (inductively coupled plasma optical emission spectrometry), in order to determine metal content. Correlations between PM10 and metal concentrations were evaluated, considering meteorological data for each site. Results showed that the average PM10 concentrations were 36 microgram m-3; 50 microgram m-3 and 74 microgram m-3 at the rural, downtown and industrial sites, respectively. The measured PM10 concentration in the industrial site exceeded the limit allowed by the Brazilian law, which is 50 microgram m-3. While industrial area had the highest concentrations of metals, such as Fe, Zn, Al, Ti, Mn, Cr, Ni, Cd and Pb, the highest concentrations of Cu and V were found in areas with higher traffic densities. Ionic species had higher concentrations in the urban site due to the proximity to the ocean. The lowest concentrations of species from anthropogenic sources, such as sulfate, nitrate and some metals (Pb, Cr, V and Ni) were measured in the rural area. Hence, studied species concentrations are influenced by site locations, but the influence by the transportation of long distance pollutants, such as biomass burning and dust from African deserts was also observed. The acid/aqueous percentiles showed that the most efficiently extracted metals in the aqueous phase were V and Ni (greater than 45 percent) while the less efficiently extracted were Al and Fe (less than 3 percent), as expected. The aqueous fraction is important to evaluate the bioavailability of metals that are associated to damage to the human health.

Planner, conservationist, forester, and geographer Emile Benton MacKaye envisioned a revolutionary, extensive foot trail that would promote the interaction between communities throughout the United States' distinctive eastern region. His 1921 plan for the Appalachian Trail (A.T.) focused on balancing the basic requirements for life in and out of the urban context by developing an ‘indigenous’ environment and developmental mold (Bower 1962, 372). However, almost a century has passed, and MacKaye's approach to the planning process, organization, environmental development, and the rural economy remains hidden beneath the mountain forest canopy extending from Maine to Georgia. Four of the forty-nine designated communities in the A.T. CommunityTM program today were analyzed to determine if and which aspects of Benton MacKaye's original vision of regional planning were achieved 100 years later. On-the-ground observations were collected through informal interactions with A.T. CommunityTM Supporters, unaffiliated businesses and organizations, and locals while traveling to each of the four A.T. regions, defined by the A.T. Conservancy (ATC). It was essential to understand if the designations transformed trailside neighborhoods into outdoor recreational and social hubs and shifted community perspectives toward the Trail and the ATC. Although most people think of the A.T. purely in terms of the opportunities it provides for outdoor and wilderness experiences, it is also perceived as a critical focus for communities' economic growth and vitality. The research reveals that the program and its complimentary ‘Supporter’ system for local businesses are ineffective. The ATC branded these trail towns. Yet, no elements of MacKaye’s vision or community improvements have truly been recognized following A.T. CommunityTM designations within the last decade. Regardless of geographic, temporal, internal, and physical aspects, this outcome was consistent in all four case study communities (Monson-ME, Harpers Ferry-Bolivar-WV, Damascus-VA, and Hot Springs-NC).

No description available.

El objetivo de esta investigación es analizar la gráfica visual y funcionalidad de los cuatro “Cuadernos de autoaprendizaje” dirigidos a niños de primaria regular en la plataforma de Aprendo en casa, los cuales fueron inicialmente desarrollados para ser usados en un formato físico, pero sus formatos digitales se utilizaron como alternativa educativa virtual durante el Estado de Emergencia sanitaria ocasionado por el Covid-19. Se parte de la hipótesis que la gráfica visual de los “Cuadernos de autoaprendizaje” cumple con su propósito comunicativo, pero la funcionalidad del formato digital del material escolar en la plataforma de Aprendo en casa, se ve interrumpida debido a su inadecuada adaptación a la virtualidad, lo cual impide el desarrollo de la dinámica educativa durante el proceso de aprendizaje de los niños. Para lograr el desarrollo de esta investigación se realizó un análisis gráfico de los “Cuadernos de autoaprendizaje”, también se realizaron entrevistas a expertos y se analizó la interacción entre niños de primaria y el material educativo ya mencionado. Los resultados muestran que el desarrollo de material educativo dirigido a niños en una dinámica virtual, debe tener en cuenta factores externos como las distracciones del entorno, ausencia del profesor o limitaciones propias de la edad de los niños, los cuales deben evitarse mediante el desarrollo de un material lo suficientemente interactivo y gráficamente eficiente que capte la atención de los escolares y además cumpla con su objetivo educativo. Se concluye que si bien el formato físico de los “Cuadernos de autoaprendizaje” puede funcionar en una dinámica presencial, su formato digital no cuenta con herramientas gráficas funcionales, lo cual interfiere durante la interacción de los niños con el material en cuestión perjudicando así su proceso de aprendizaje. / The objective of this research is to analyze the visual graphics and functionality of the four “Cuadernos de autoaprendizaje” directed at regular elementary school children on the Aprendo en casa platform, which were initially developed to be used in a physical format, but their digital formats were used as a virtual educational alternative during the State of Health Emergency caused by Covid-19. It is based on the hypothesis that the visual graphics of the “Cuadernos de autoaprendizaje” fulfills its communicative purpose, but the functionality of the digital format of the school material in the Aprendo en casa platform is interrupted due to its inadequate adaptation to virtuality, which prevents the development of educational dynamic during the learning process of children. To achieve the development of this research, a graphic analysis of the “Cuadernos de autoaprendizaje” was carried out, interviews with experts were also carried out and the interaction between primary school children and the educational material already mentioned was analyzed. The results show that the development of educational material aimed at children in a virtual dynamic must take into account external factors such as distractions from the environment, absence of the teacher or limitations of the children's age, which must be avoided through the development of a sufficiently interactive and graphically efficient material that captures the attention of schoolchildren and also fulfills its educational objective. It is concluded that although the physical format of the “Cuadernos de autoaprendizaje” can work in a face-to-face dynamic, its digital format doesn’t have functional graphic tools, which interferes during the interaction of children with the material in question, thus harming their process learning. / Trabajo de investigación

Between 2007 and 2016, there was a rescue archeological research done by the Institute of Archaeology of the CAS in the locality of Brandýs nad Labem-Vrábí at the site "U Vodojemu", where, above other, a large settlement from the Early Bronze Age was uncovered. Based on the ceramics, it is possible to classify it chronologically as the early period of the Únětice culture, typologically then, it is possible to associate the ceramics found with the post-classical phase of the Únětice culture. The discovery of the U-shaped ditch adjacent to the ground edge of the Hrušovský stream indicates that the settlement was fortified for a certain time, approximately at the beginning of the 2nd millennium BC. Based on the nature of the ditch filling, of the ceramic findings with analogies in the beginning of the tumulus culture, as well as of the carbon dating, it is obvious that the settlement served further on after the downfall of this fortification, too, and thus for another 100 years at least. In the context of another very numerous, contemporaneous findings in the cadaster of Brandýs nad Labem, it is visible that the site "U Vodojemu" used to be a part of a large settlement agglomeration. A detailed mapping of the Early Bronze Age findings from the district of the north-east part of Central Bohemia has...