Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-08-25T20:42:57Z No. of bitstreams: 1 JamileMarinhoBezerraDeOliveiraMoura_TESE.pdf: 18433655 bytes, checksum: e3b33cd1bc7a5e8e3f1a6cdd83ebdcbd (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-08-26T20:02:15Z (GMT) No. of bitstreams: 1 JamileMarinhoBezerraDeOliveiraMoura_TESE.pdf: 18433655 bytes, checksum: e3b33cd1bc7a5e8e3f1a6cdd83ebdcbd (MD5) / Made available in DSpace on 2016-08-26T20:02:15Z (GMT). No. of bitstreams: 1 JamileMarinhoBezerraDeOliveiraMoura_TESE.pdf: 18433655 bytes, checksum: e3b33cd1bc7a5e8e3f1a6cdd83ebdcbd (MD5) Previous issue date: 2016-02-25 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / As neoplasias de gl?ndulas salivares exibem uma ampla variedade de comportamento biol?gico e grande diversidade morfol?gica, e esta heterogeneidade inerente a este grupo de tumores suscita o interesse em pesquisar estas les?es. As c?lulas-tronco s?o a principal fonte para a gera??o e manuten??o da diversidade celular e homeostase do tecido, dist?rbios na regula??o destas c?lulas podem levar ? produ??o de c?lulas-tronco alteradas, denominadas de c?lulas-tronco tumorais, que possuem potencial proliferativo e capazes de originar e/ou manter o tumor. Pesquisas acerca das c?lulas-tronco tumorais e das prote?nas a elas associadas em algumas neoplasias orais t?m sido desenvolvidas, no entanto, o papel destas em neoplasias de gl?ndulas salivares n?o est? ainda bem estabelecido. Desta forma, o objetivo deste estudo foi identificar c?lulas do par?nquima tumoral que expressam marcadores de c?lulas-tronco tumorais, atrav?s da avalia??o da imunoexpress?o do OCT4 e CD44, em uma s?rie de casos de neoplasias de gl?ndulas salivares. A amostra foi constitu?da por 20 adenomas pleom?rficos, 20 carcinomas mucoepiderm?ides e 20 carcinomas aden?ides c?sticos localizados nas gl?ndulas salivares menores e maiores. Todos os casos estudados exibiram express?o positiva para OCT4 e CD44, sendo observado que para ambos marcadores, as neoplasias localizadas nas gl?ndulas salivares maiores exibiram maior imunomarca??o quando comparada com as les?es das gl?ndulas salivares menores apresentando diferen?a estatisticamente significativa (p=<0,001). Na amostra total e no grupo das gl?ndulas salivares menores, as neoplasias malignas exibiram maior imunorreatividade para OCT4 do que o adenoma pleom?rfico. No entanto, n?o foi encontrada diferen?as estatisticamente significativas de imunoexpress?es entre as les?es e entre suas classifica??es/grada??es histomorfol?gicas. Analisando a correla??o entre as imunoexpress?es de OCT4 e CD44 foi observada uma correla??o positiva moderada (r=0,444) com signific?ncia estat?stica entre os mesmos. A elevada express?o de OCT4 e CD44 pode indicar que estas prote?nas desempenham papel importante na identifica??o de c?lulas-tronco tumorais, permitindo uma previs?o do comportamento biol?gico das neoplasias de gl?ndula salivar, apresentando n?veis menores em tumores benignos e maiores nos tumores malignos. / Salivary gland neoplasms exhibit a wide variety of biological behavior and a high morphological diversity raises the interest in researching these lesions. The stem cells are the main source for the generation and maintenance of cell diversity, disorders in the regulation of these cells can lead to the production of altered stem cells, termed cancer stem cells capable of generate the tumor. Researches on cancer stem cells and associated proteins have been developed in some oral cancers; however, their role in salivary gland neoplasms is not well established. Thus, the aim of this study was to identify the tumor parenchyma cells exhibiting stem cell characteristics, by evaluating the immunoreactivity of OCT4 and CD44, in a number of cases of salivary gland neoplasms. The sample consisted of 20 pleomorphic adenomas, 20 mucoepidermoid carcinomas and 20 adenoid cystic carcinoma located in minor and major salivary glands. The expression of OCT4 and CD44 was evaluated by the percentage of positive cells (PP) and the intensity of expression (IE), it is realized the sum of the scores, resulting in the total score immunostaining (PIT) ranging 0-7. All studied cases showed positive expression of OCT4 and CD44 and higher values than the control groups. It was observed that for OCT4 luminal cells and non-luminal were immunostained in the case of pleomorphic adenomas and adenoid cystic carcinoma. Already the immunoreactivity of CD44 was particularly evident in the non-luminal cells of these lesions. In mucoepidermoid carcinomas for both markers, there was immunoreactivity in squamous and intermediate cells and absence of staining mucous cells. For both markers, a statistically significant higher immunostaining was verified in neoplasms located in the major salivary glands compared with lesions in the minor salivary (p<0.001). At the total sample and in the group of minor salivary glands, malignant neoplasms exhibited higher immunoreactivity for OCT4 than pleomorphic adenoma. However, there was no statistically significant difference between the lesions and between their classifications histomorphologic. Analyzing the correlation between OCT4 and CD44 immunoexpressions, a statistically significant moderate positive correlation (r = 0.444) was observed. The high expression of OCT4 and CD44 may indicate that these proteins play an important role in identifying cancer stem cells, allowing a prediction of biological behavior of salivary gland neoplasms.

Submitted by Jos? Henrique Henrique (jose.neves@ufvjm.edu.br) on 2017-03-24T21:23:22Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) monique_rocha_almeida.pdf: 4635045 bytes, checksum: 2d203824a390ae82a5006e68b621c98b (MD5) / Approved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-04-20T19:40:16Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) monique_rocha_almeida.pdf: 4635045 bytes, checksum: 2d203824a390ae82a5006e68b621c98b (MD5) / Made available in DSpace on 2017-04-20T19:40:16Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) monique_rocha_almeida.pdf: 4635045 bytes, checksum: 2d203824a390ae82a5006e68b621c98b (MD5) Previous issue date: 2016 / Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) / A convers?o de energia solar em energia qu?mica usando c?lulas fotoeletroqu?micas ? uma estrat?gia interessante para armazenar energia. C?lulas fotoeletroqu?micas s?o dispositivos constitu?dos de fotoeletrodos semicondutores que absorvem luz com energia maior ou igual a energia de bandgap do semicondutor e geram cargas reativas (el?trons e buracos) na superf?cie dos fotoeletrodos capazes de promover a redu??o e oxida??o da ?gua em H2 e O2, respectivamente. Nesta disserta??o, quatro fotoeletrodos de g-C3N4, g-C3N4/Cu1%, g- C3N4/Cu5% e Cu2O/CuO foram preparados com o objetivo de desenvolver uma c?lula fotoeletroqu?mica para clivagem da ?gua em H2 e O2 de forma espont?nea. As medidas de difratometria de raios X confirmaram a presen?a das fases g-C3N4 e Cu2O/CuO nos fotoeletrodos. As imagens de MEV mostraram que os materiais ? base de g-C3N4 possuem morfologia do tipo esponja, enquanto a heterojun??o Cu2O/CuO ? formada por nanopart?culas de forma indefinida. Medidas de reflect?ncia difusa mostraram que o acoplamento do g-C3N4 e Cu2O/CuO resulta em uma melhora significativa na absor??o ?ptica dos fotoeletrodos. Medidas de ?rea espec?fica indicaram que os nanomateriais ? base de g-C3N4 tem alta ?rea superficial (?100 m2 g?1), enquanto a ?rea espec?fica da heterojun??o Cu2O/CuO foi de 17 m2 g?1. Os resultados de redu??o ? temperatura programada evidenciaram a forma??o das heterojun??es. Os testes fotoeletroqu?micos de produ??o de O2 a partir da ?gua usando luz vis?vel indicaram que em potenciais an?dicos, apenas o fotoanodo de g-C3N4 foi est?vel apresentando uma densidade de fotocorrente de 16 ?A cm?2 que corresponde a uma efici?ncia de convers?o de luz de 0,014%. Em potenciais cat?dicos, a maior densidade de fotocorrente (60 ?A cm?2) foi obtida para o fotoeletrodo Cu2O/CuO. A efici?ncia de convers?o de luz do fotocatodo de Cu2O/CuO foi de 0,029%. Com base nos dados obtidos, uma c?lula fotoeletroqu?mica p-n foi constru?da usando a heterojun??o Cu2O/CuO como fotocatodo e g- C3N4 como fotoanodo. Esta c?lula gerou uma densidade de fotocorrente in operando de 0,62 ?A cm?2 e uma fotovoltagem de 0,62 V. A efici?ncia de convers?o solar da fotoc?lula foi de 0,004% sob irradia??o de luz vis?vel. Apesar da baixa efici?ncia obtida, espera-se que esta disserta??o possa servir de inspira??o para o desenvolvimento de novos dispositivos fotoeletroqu?micos para clivagem da ?gua em H2 e O2, usando luz vis?vel. / Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016. / The conversion of solar energy into chemical energy using photoelectrochemical cells is an interesting strategy to store energy. Photoelectrochemical cells are made up of semiconductor photoelectrodes that absorb light with energy equal or higher than the bandgap energy of the semiconductor to generate reactive charges (electrons and holes) on the surface of the photoelectrodes, which can promote the oxidation and reduction reactions of water to form H2 and O2, respectively. In this dissertation, four photoelectrodes of g-C3N4, g-C3N4/Cu1%, g- C3N4/Cu5%, and Cu2O/CuO were prepared in order to develop a photoelectrochemical cell for spontaneous water splitting into H2 and O2. The X-ray diffraction patterns confirmed the presence of g-C3N4 and Cu2O/CuO phases in the photoelectrodes. The SEM images showed that the materials based on g-C3N4 have sponge-like morphology, whereas the Cu2O/CuO heterojunction is formed by nanoparticles with undefined shapes. Diffuse reflectance measurements showed that coupling g-C3N4 and Cu2O/CuO results in a significant improvement in optical absorption of the photoelectrodes. Surface area measurements indicated that the nanomaterials based on g-C3N4 have high surface areas (?100 m2 g?1), while the specific area for the Cu2O/CuO heterojunction was 17 m2 g?1. The temperature programmed reduction results evidenced the formation of the heterojunctions. The photoelectrochemical assays of O2 production from water using visible light indicated that at anodic potentials, only the photoanode g-C3N4 was stable showing a photocurrent density of 16 ?A cm?2, which corresponds to a light conversion efficiency of 0.014%. At cathodic potentials, the higher photocurrent density (60 ?A cm?2) was obtained for the Cu2O/CuO photoelectrode. The light conversion efficiency of the Cu2O/CuO photocathode was 0.029%. Based on the obtained data, a p-n photoelectrochemical cell was constructed using the Cu2O/CuO heterojunction as the photocathode and g-C3N4 as the photoanode. This photocell generated a photocurrent density in operando of 0.62 ?A cm?2 and photovoltage of 0.62 V. The light conversion efficiency of the photocell was 0.004% under visible light irradiation. Despite the low efficiency obtained for the p-n photocell, it is expected that this dissertation may serve of inspiration for the development of new photoelectrochemical devices for water splitting into H2 and O2 using visible light.

Made available in DSpace on 2015-03-03T14:02:14Z (GMT). No. of bitstreams: 1 JussaraMCM_Tese.pdf: 1097860 bytes, checksum: 122136921e4dddbf8d0e434cbbbd3653 (MD5) Previous issue date: 2009-12-03 / The activation of hepatic stellate cells (HSC) is considered the most important event in hepatic fibrogenesis. The precise mechanism of this process is unknown in autoimmune hepatitis (AIH), and more evidence is needed on the evolution of fibrosis. The aim of this study was to assess these aspects in children with type 1 AIH. We analyzed 16 liver biopsy samples from eight patients, paired before treatment and after clinical remission, performed an immunohistochemical study with anti-actin smooth muscle antibody and graded fibrosisand inflammation on a scale of 0:4 (Batts and Ludwig scoring system). We observedthere was no significant reduction in fibrosis scores after 24? 18 months (2.5 ? 0.93 vs. 2.0? 0.53, P = 0.2012). There was an important decrease in inflammation: portal (2.6 ?0.74 vs. 1.3? 0.89, P = 0.0277), periportal/periseptal (3.0 ?0.76 vs. 1.4 ? 1.06, P = 0.0277), and lobular (2.8 ? 1.04 vs. 0.9? 0.99, P =0.0179). Anti-actin smooth muscle antibodies were expressed in the HSC of the initial biopsies (3491.93 ?2051.48 lm2), showing a significant reduction after remission (377.91 ?439.47 lm2) (P = 0.0117). HSC activation was demonstrated in the AIH of children. The reduction of this activation after clinical remission, which may precede a decrease in fibrosis, opens important perspectives in the follow-up of AIH. / A ativa??o das c?lulas hep?ticas estreladas (CHE) ? considerada o evento mais importante na fibrog?nese hep?tica. Na hepatite autoimune (HAI) este mecanismo ? desconhecido e maiores evid?ncias s?o necess?rias quanto ? evolu??o da fibrose. O objetivo desse estudo foi avaliar a ativa??o das CHE e a evolu??o da fibrose hep?tica em crian?as portadoras de HAI tipo 1. Foram analisadas 16 bi?psias hep?ticas pareadas de oito pacientes, antes do tratamento e ap?s a remiss?o cl?nica atrav?s de estudo imuno-histoqu?mico com anticorpo anti-??-actina de m?sculo liso e realizada a grada??o da fibrose e da inflama??o empregando-se o sistema de escores de Batts e Ludwig (0-4). N?o houve significante redu??o nos escores de fibrose ap?s intervalo de tempo de 24? 18 meses entre as bi?psias (2,5 ? 0,93 vs. 2,0? 0,53, P = 0,2012). Observou-se redu??o significante na inflama??o: portal (2,6?0,74 vs. 1,3? 0,89, P = 0,0277), periportal/perisseptal (3,0 ?0,76 vs. 1,4 ? 1,06, P =0,0277) e lobular (2,8 ? 1,04 vs. 0,9? 0,99, P =0,0179). A -actina de m?sculo liso nas CHE foi expressa em bi?psias hep?ticas iniciais (3491,93 ?2051,48 ?m2) e mostrou significante redu??o ap?s a remiss?o cl?nica (377,91 ?439,47 ?m2) (P = 0,0117). A ativa??o de CHE foi demonstrada em crian?as portadoras de HAI tipo 1. A redu??o de sua ativa??o ap?s remiss?o cl?nica, a qual pode preceder a redu??o da fibrose, abre importantes perspectivas no follow-up da HAI

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2015-12-04T19:34:33Z No. of bitstreams: 1 NilmaraDeOliveiraAlves_TESE.pdf: 4420508 bytes, checksum: 970409d7d94b116854a2e3e7da3725ee (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2015-12-09T23:25:19Z (GMT) No. of bitstreams: 1 NilmaraDeOliveiraAlves_TESE.pdf: 4420508 bytes, checksum: 970409d7d94b116854a2e3e7da3725ee (MD5) / Made available in DSpace on 2015-12-09T23:25:19Z (GMT). No. of bitstreams: 1 NilmaraDeOliveiraAlves_TESE.pdf: 4420508 bytes, checksum: 970409d7d94b116854a2e3e7da3725ee (MD5) Previous issue date: 2014-08-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / A Amaz?nia representa mais da metade das florestas tropicais remanescentes no planeta e compreende a maior biodiversidade do mundo, correspondendo aproximadamente a 60% do territ?rio brasileiro. Entretanto, o desmatamento e as queimadas que ocorrem na regi?o t?m causado s?rios preju?zos para a popula??o que est? sendo exposta. Diante desta situa??o, o objetivo do presente estudo foi avaliar os compostos qu?micos assim como os efeitos celulares e moleculares ap?s a exposi??o ao material org?nico extra?do do material particulado menor que 10 ?m (MP10) na regi?o Amaz?nica. Com rela??o ? composi??o qu?mica, a an?lise dos n-alcanos mostrou um predom?nio da influ?ncia antr?pica no per?odo de queimadas na regi?o. Al?m disso, observou-se um predom?nio dos monossacar?deos marcadores da queima de biomassa. Tamb?m foram identificados os Hidrocarbonetos Polic?clicos Arom?ticos (HPA) e os seus derivados nas amostras coletadas na Amaz?nia. Os dados das concentra??es dos HPA permitiram calcular o BaP-equivalente e observou-se que o dibenzo(a)antraceno contribui com 83% para o potencial risco carcinog?nico. J? para o potencial risco mutag?nico, o benzo(a)pireno ? o HPA que apresenta uma maior contribui??o nesta an?lise. Pode-se destacar que o reteno foi o HPA mais abundante. Este composto foi considerado genot?xico, al?m de causar morte por necrose nas c?lulas estudadas. Nas an?lises biol?gicas, os dados mostraram que o MP10 org?nico ? capaz de causar altera??es gen?ticas tanto em c?lulas vegetais como em c?lulas do pulm?o humano. Estes danos levaram a uma parada na fase G1 no ciclo das c?lulas expostas, aumentando a express?o das prote?nas p53 e p21. Al?m disso, o MP causou morte celular por apoptose, aumentando a marca??o da histona ?-H2AX. Com resultados bem evidentes, o MP inal?vel tamb?m causou morte por necrose nas c?lulas do pulm?o humano. Diante destes resultados, ? importante enfatizar a import?ncia da redu??o e um melhor controle da queima de biomassa na regi?o Amaz?nica. Afinal, como descrito recentemente pela Organiza??o Mundial de Sa?de, pode-se afirmar que a redu??o da polui??o do ar poder? salvar milh?es de vidas. / The Amazon holds over half of the planet's remaining tropical forests and comprises the largest biodiversity in the world, accounting for approximately 60 % of the Brazilian territory. However, deforestation fires in the region causes serious problems to exposed human. The aim of this study was to evaluate the chemical compounds as well as the cellular and molecular effects after exposure to organic material extracted from particulate matter less than 10 ?m (PM10) in the Amazon region. As for the chemical composition, n-alkanes analysis showed a prevalence of anthropogenic influence during the fires in the region. In addition, there was a predominance of monosaccharides from biomass burning markers. Also, the Polycyclic Aromatic Hydrocarbons (PAH) and their derivatives have also been identified in samples collected in the Amazon. By using the PAH concentrations was possible to calculate the BaP-equivalent and it was found that the dibenz(a) anthracene contributes with 83% to potential carcinogenic risk. As for the potential mutagenic risk, the benzo (a) pyrene is the HPA that has a major contribution in this analysis. It may be noted that the retene was the most abundant PAH. This compound was genotoxic and cause death by necrosis in the human lung cells. In biological tests, the data showed that organic PM10 is capable of causing genetic damage in both plant cells and in human lung cells. This damage cause an arrest in the G1 phase of the cell cycle exposed, increasing the expression of p53 and p21. Additionally, the PM10 caused cell death by apoptosis, increasing the foci of histone ?- H2AX. Given these results, it is important to emphasize the reduction and better control of biomass burning in the Amazon region thus improving the quality of health of the population being exposed. As clearly stated recently by the World Health Organization, the reduction of air pollution could save millions of lives annually.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-02-29T22:55:50Z No. of bitstreams: 1 AdrianaCostaDeSouzaMartinsCamara_TESE.pdf: 3396087 bytes, checksum: b8e35eee4bb9ca8e4392251f05ab7935 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-02T19:21:41Z (GMT) No. of bitstreams: 1 AdrianaCostaDeSouzaMartinsCamara_TESE.pdf: 3396087 bytes, checksum: b8e35eee4bb9ca8e4392251f05ab7935 (MD5) / Made available in DSpace on 2016-03-02T19:21:41Z (GMT). No. of bitstreams: 1 AdrianaCostaDeSouzaMartinsCamara_TESE.pdf: 3396087 bytes, checksum: b8e35eee4bb9ca8e4392251f05ab7935 (MD5) Previous issue date: 2015-02-26 / Altera??es epiteliais observadas em queilites act?nicas (QA) e carcinomas epiderm?ides de l?bio inferior (CELI) s?o causadas principalmente pela exposi??o cr?nica aos raios ultra-violetas (UV) e s?o estudadas usando diferentes marcadores imuno-histoqu?micos procurando avaliar o processo da carcinog?nese. O objetivo deste estudo foi avaliar comparativamente a express?o das prote?nas Ki-67 e IMP-3 em QA e CELI a fim de contribuir com informa??es adicionais sobre a carcinog?nese em l?bio inferior. Foram estudados 33 casos de QA e 33 casos de CELI, sendo analisadas as caracter?sticas cl?nico-patol?gicas e a imunoexpress?o do Ki-67 e IMP-3. A an?lise imuno-histoqu?mica do Ki-67 se deu atrav?s da determina??o do ?ndice de prolifera??o (IP) e subsequente classifica??o dos casos de acordo com os escores : 0 (0% de c?lulas positivas), +1(?30%), +2 (>30% a ?60%) e +3 (>60%). Para aplica??o dos testes estat?sticos os casos foram classificados em: sem marca??o (escore 0), baixa express?o (escore +1) e alta express?o (escores +2 e +3). Para a express?o do IMP-3, foi estabelecido o percentual de c?lulas epiteliais imunomarcadas, sendo atribu?dos os escores: 0 (correspondeu a 0%), +1 (at? 30% das c?lulas positivas); +2 (entre 30% a 60% de c?lulas imunomarcadas) e +3 (acima de 60% das c?lulas positivas). Foram utilizados os testes estat?sticos Qui-quadrado de Pearson, Mann-Whitney e Wilcoxon. O n?vel de signific?ncia adotado foi de 5%. A maioria dos caos de QA foi do sexo masculino (78,8%), com m?dia de idade de 50 anos e dos casos de CELI tamb?m predominou o sexo masculino (69,89%) com m?dia de 62 anos. O Ki-67 se expressou em todos os casos de QAs bem como nos casos de CELI, predominando nas duas les?es o escore 2, correspondendo a 81,8% dos casos nas QAs e 54,5% nos CELI. A express?o do IMP-3 nas QAs ocorreu em 72,7% dos casos, com predomin?ncia em 36,3% dos casos do escore 1. J? nos CELI o IMP-3 se expressou em 60,6% dos casos, com predomin?ncia em 27,3% dos casos do escore 3. Estes resultados nos permite concluir que a express?o do IMP3 e da atividade proliferativa s?o eventos precoces na carcinog?nese de l?bio inferior independente do estado da altera??o. / Epithelial changes observed in actinic cheilitis (AC) and squamous cell carcinoma of the lower lip (LLSCC) are mainly caused by chronic exposure to ultraviolet rays (UV) and are studied using different immunohistochemical markers trying to evaluate the process of carcinogenesis. The objective of this study was to comparatively evaluate the expression of Ki-67 proteins and IMP-3 in AC and LLSCC to contribute with additional information on carcinogenesis in lower lip. A total of 33 cases of AC and 33 cases of LLSCC were studied, analyzed the clinical and pathological features and immunostaining of Ki-67 and IMP-3. Immunohistochemical analysis of Ki-67 was made through the determination of the proliferation index (PI) and subsequent classification of the cases according to the scores: 0 (0% positive cells) +1 (?30%) + 2 (> 30% and ?60%) and +3 (> 60%). For statistical tests cases were classified as unmarked (score 0), low expression (score +1) and high expression (scores +2 and +3). For the expression of IMP-3, the percentage of immunostained epithelial cells was established, and assigned scores: 0 (corresponding to 0%), +1 (up to 30% of positive cells); +2 (From 30% to 60% of immunostained cells) and +3 (over 60% of positive cells). Statistical tests chi-square test, Mann-Whitney and Wilcoxon were used. The significance level was 5%. Most AC chaos was male (78.8%) with mean age of 50 years and cases of LLSCC also were male (69.89%) with an average of 62 years. The Ki-67 was expressed in all cases of AC and in cases of LLSCC, predominantly in the two injuries the score 2, corresponding to 81.8% of cases in ACs and 54.5% in the CELI. The expression of IMP-3 in ACs occurred in 72.7% of cases, predominantly in 36.3% of LLSCC cases score 1. Already in the IMP-3 was expressed in 60.6% of cases, especially in 27.3% of the score of the cases 3. These results allow us to conclude that the expression of IMP3 and proliferative activity are early events in carcinogenesis independently lower lip state of change.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-09T23:59:33Z No. of bitstreams: 1 AntonnyoPalmiellyDiogenesLima_DISSERT.pdf: 2603626 bytes, checksum: 436271fd3d1c04603beb317bd4eb74b4 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-03-15T00:17:56Z (GMT) No. of bitstreams: 1 AntonnyoPalmiellyDiogenesLima_DISSERT.pdf: 2603626 bytes, checksum: 436271fd3d1c04603beb317bd4eb74b4 (MD5) / Made available in DSpace on 2016-03-15T00:17:56Z (GMT). No. of bitstreams: 1 AntonnyoPalmiellyDiogenesLima_DISSERT.pdf: 2603626 bytes, checksum: 436271fd3d1c04603beb317bd4eb74b4 (MD5) Previous issue date: 2015-04-24 / O transplante renal ? a melhor forma de tratamento para indiv?duos que perderam a fun??o do rim. Pacientes transplantados renais necessitam de rigoroso esquema imunossupressor para evitar rejei??o. Nesse processo c?lulas T helper do sistema imunol?gico exercem papel chave na resposta contra o enxerto, sendo as c?lulas Th17 recentemente investigadas por produzirem IL-17, uma potente citocina pr?-inflamat?ria cujo papel na rejei??o tamb?m vem sendo descrito. O aumento da express?o de c?lulas Th17 tem importante associa??o ao desenvolvimento da rejei??o no microambiente renal, no entanto o prov?vel mecanismo ainda n?o est? bem compreendido. Esse estudo teve como objetivo avaliar a resposta Th17 a partir da influ?ncia exercida pelo eixo quimiot?tico CCR6/CCL20 e por variantes gen?ticas na IL- 17 e seu receptor IL-17RA. Para isso, realizou-se um estudo caso controle envolvendo 148 pacientes transplantados do Hospital Universit?rio Onofre Lopes/UFRN no qual se avaliou por imunohistoqu?mica a express?o proteica da IL-17 e das quimiocinas CCR6/CCL20 e por PCR-RFLP as variantes gen?ticas em IL17A e IL17RA. Nossos resultados demonstraram n?o haver influ?ncia dos polimorfismos g?nicos sobre o desfecho do enxerto ou sobre a express?o proteica da IL-17. No microambiente do enxerto renal encontramos v?rias fontes produtoras de IL-17: c?lulas epiteliais tubulares, c?lulas glomerulares, neutr?filos e c?lulas do infiltrado intersticial, por sua vez a express?o do eixo quimiot?tico CCR6/CCL20 ficou restrita a c?lulas do epit?lio tubular. Houve uma leve correla??o linear positiva entre a presen?a de IL-17 e a express?o do eixo quimiot?tico CCR6/CCL20 no microambiente do enxerto renal. Acreditamos que, aliado aos nossos resultados, estudos posteriores com aumento do ?n? amostral e um maior controle sobre as vari?veis que envolvem a obten??o do esp?cime renal, podem determinar com maior clareza a influ?ncia exercida pelo eixo quimiot?tico CCR6/CCL20 e a exercida por polimorfismos gen?ticos em citocinas, sobre o controle da resposta Th17 nos processos de rejei??o ao aloenxerto renal. / Kidney transplantation is the best treatment for patients who have lost kidney function. Renal transplant patients require accurate immunosuppressive drugs to prevent rejection. In this process T helper cells of the immune system perform key role in the immune response to the graft, and recently the Th17 cells has been investigated by production of IL-17 potent proinflammatory cytokine whose role in the rejection has also been described. Increased of Th17 cell expression has an important association with the development of rejection in renal microenvironment, however the likely mechanism is not well understood. This study aimed to evaluate the Th17 response from the influence of the chemotactic axis CCR6/CCL20 and genetic variants in IL-17 and IL-17RA. We conducted a case-control study involving 148 patients transplanted at the University Hospital Onofre Lopes/UFRN in which assessed by immunohistochemistry protein expression of IL-17 and chemokines CCR6/CCL20 and by PCR-RFLP genetic variants in IL17A and IL17RA. Our results showed no influence of genetic polymorphisms on the outcome of the graft or the protein expression of IL-17. In renal graft microenvironment found several sources producing IL-17: tubular epithelial cells, glomerular cells, neutrophils and cell interstitial infiltration, in turn the expression of chemotactic axis CCR6/CCL20 was restricted to the tubular epithelium cells. There was a slight positive linear correlation between the presence of IL-17 and expression of chemotactic axis CCR6/CCL20 in the microenvironment of renal graft. Therefore, we believe that, combined with our results, further studies with increased "n" sample and greater control over the variables involved in obtaining the renal specimen, can determine more clearly the influence of chemotactic axis CCR6 / CCL20 and polymorphisms in cytokines related to Th17 profile on the control of this cell subtype response in rejection processes to renal allograft.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-03-31T22:25:53Z No. of bitstreams: 1 CarolineGomesMoura_DISSERT.pdf: 2102873 bytes, checksum: c954d6aaea1a58ccd102a726f8bae793 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-04-01T23:05:31Z (GMT) No. of bitstreams: 1 CarolineGomesMoura_DISSERT.pdf: 2102873 bytes, checksum: c954d6aaea1a58ccd102a726f8bae793 (MD5) / Made available in DSpace on 2016-04-01T23:05:31Z (GMT). No. of bitstreams: 1 CarolineGomesMoura_DISSERT.pdf: 2102873 bytes, checksum: c954d6aaea1a58ccd102a726f8bae793 (MD5) Previous issue date: 2015-06-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / O PSFC (Pr0.5Sr0.5Fe1-xCuxO3-?) ? um novo ?xido misto com estrutura perovskita que vem sendo estudado para aplica??o como catodo de c?lulas a combust?vel de ?xido s?lido de temperatura intermedi?ria (600-750 ?C), principalmente devido a sua elevada condutividade i?nica e eletr?nica abaixo de 800 ?C. Neste trabalho, p?s de PSFC com duas composi??es diferentes (Pr0,5Sr0,5Fe0,8Cu0,2O3-PSFC5582 e Pr0,5Sr0,5Fe0,6Cu0,4O3-PSFC5564) foram sintetizados pelo m?todo dos citratos utilizando uma nova rota. Os p?s obtidos foram caracterizados por an?lise t?rmica (Termogravimetria e Calorimetria Diferencial Explorat?ria), e os materiais calcinados a 800, 900 e 1000 ?C por 5h foram analisados por difratometria de raios X (DRX), com refinamento Rietveld dos dados de difra??o e dilatometria. Filmes de composi??o PSFC5582 foram obtidos por serigrafia do p? calcinado a 1000 ?C. Os filmes foram depositados em substrato de c?ria dopada com gadol?nia (CGO) e sinterizados a 1050 ?C por 2h. O desempenho eletroqu?mico dos eletrodos foi avaliado por espectroscopia de imped?ncia e a interface eletrodo/eletr?lito foi observada por microscopia eletr?nica de varredura (MEV). A resist?ncia espec?fica por ?rea (ASR) foi 0,44 ?.cm? a 800 ?C, um valor pouco inferior aos reportados na literatura para catodos contendo cobalto. Os coeficientes de expans?o t?rmica (CET) do PSFC de ambas as composi??es variaram entre 13 e 15 x 10-6 ?C-1 , numa faixa de temperatura de 200 a 650 ?C, demonstrando a boa compatibilidade t?rmica destes catodos com eletr?litos de Ce0,9Gd0,1O1,95 (CET = 12 x 10-6 ?C). / The PSFC (Pr0.5Sr0.5Fe1-xCuxO3-?) is a new mixed oxide perovskite and has been studied and evaluated the cathode materials for intermediate temperature solid oxide fuel cells (IT-SOFCs), mainly due to its good compatibility with the electrolyte (CGO) and its high ionic conductivity and electronic in intermediate temperature. In this work, PSFC powders with two different compositions (Pr0,5Sr0,5Fe0,8Cu0,2O3- PSFC5582 and Pr0,5Sr0,5Fe0,6Cu0,4O3-PSFC5564) were synthesized by the citrate method using a new route. The powders obtained were characterized by thermal analysis (Differential Scanning Calorimetry and Thermogravimetry), and the material calcined at 800, 900 and 1000 ?C for 5h were analyzed by X-ray diffractometry (XRD), with the Rietveld refinement of the diffraction data and dilatometry. PSFC5582 composite films were obtained by screen printing of powder calcined at 1000 ?C. The films were deposited on substrate ceria doped with gadolinia (CGO) and then sintered at 1050 ?C for 2h. The electrochemical performance of the electrodes was evaluated by impedance spectroscopy and the interface electrode/electrolyte was observed by scanning electron microscopy (SEM). The specific resistance area (ASR) was 0.44 ?.cm? at 800 ?C, slightly lower than those reported in the literature for cathodes containing cobalt. The thermal expansion coefficients of both the PSFC compositions were obtained and varied between 13 and 15 x 10-6 ?C-1 , in a temperature range of 200 to 650 ?C, demonstrating the good thermal compatibility of cathodes with Ce0,9Gd0,1O1,95 electrolytes (CET = 12 x 10-6 ?C).

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-05-03T23:02:28Z No. of bitstreams: 1 DorgivalAlbertinoDaSilvaJunior_TESE.pdf: 6053241 bytes, checksum: d9642e4cc70e97ccb8ad128ccb256cf5 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-05-05T21:01:59Z (GMT) No. of bitstreams: 1 DorgivalAlbertinoDaSilvaJunior_TESE.pdf: 6053241 bytes, checksum: d9642e4cc70e97ccb8ad128ccb256cf5 (MD5) / Made available in DSpace on 2016-05-05T21:01:59Z (GMT). No. of bitstreams: 1 DorgivalAlbertinoDaSilvaJunior_TESE.pdf: 6053241 bytes, checksum: d9642e4cc70e97ccb8ad128ccb256cf5 (MD5) Previous issue date: 2015-08-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / Esta tese desenvolve uma nova t?cnica para projetos de microestruturas de comp?sitos pelo processo de Otimiza??o Topol?gica, com objetivo de maximizar a rigidez, fazendo uso do M?todo da Energia de Deforma??o e utilizando um esquema de refino h-adaptativo visando obter uma melhor defini??o do contorno topol?gico da microestrutura. Isso ? feito ao se distribuir material de forma otimizada em uma regi?o de projeto preestabelecida denominada como C?lula Base. Neste trabalho, o M?todo dos Elementos Finitos ? utilizado para descri??o do dom?nio e para solu??o da equa??o de governo. A malha ? refinada de forma iterativa de modo que o refino da malha de elementos finitos ? feito em todos os elementos que representem materiais s?lidos e todos os elementos vazios que contenham ao menos um n? em uma regi?o de material s?lido. O elemento finito escolhido para o modelo de aproxima??o ? o triangular linear de tr?s n?s. J? para a resolu??o do problema de programa??o n?o linear com restri??es foi utilizado o M?todo Lagrangiano Aumentado e um algoritmo de minimiza??o com base na dire??o do tipo Quasi-Newton e das condi??es de Armijo-Wolfe auxiliando no processo de descida. A C?lula Base que representa o comp?sito ? encontrada a partir da equival?ncia entre um material fict?cio e um material preescrito, distribu?do de forma ?tima na regi?o de projeto. A utiliza??o do M?todo da Energia de Deforma??o se justifica por proporcionar menor custo computacional devido a uma formula??o mais simplificada do que o tradicional M?todo de Homogeneiza??o. Os resultados s?o apresentados com mudan?a na prescri??o de deslocamento, com mudan?a na restri??o de volume e a partir de v?rios valores iniciais das densidades relativas. / This thesis develops a new technique for composite microstructures projects by the Topology Optimization process, in order to maximize rigidity, making use of Deformation Energy Method and using a refining scheme h-adaptative to obtain a better defining the topological contours of the microstructure. This is done by distributing materials optimally in a region of pre-established project named as Cell Base. In this paper, the Finite Element Method is used to describe the field and for government equation solution. The mesh is refined iteratively refining so that the Finite Element Mesh is made on all the elements which represent solid materials, and all empty elements containing at least one node in a solid material region. The Finite Element Method chosen for the model is the linear triangular three nodes. As for the resolution of the nonlinear programming problem with constraints we were used Augmented Lagrangian method, and a minimization algorithm based on the direction of the Quasi-Newton type and Armijo-Wolfe conditions assisting in the lowering process. The Cell Base that represents the composite is found from the equivalence between a fictional material and a preescribe material, distributed optimally in the project area. The use of the strain energy method is justified for providing a lower computational cost due to a simpler formulation than traditional homogenization method. The results are presented prescription with change, in displacement with change, in volume restriction and from various initial values of relative densities.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-06-23T21:59:34Z No. of bitstreams: 1 KivyaDiasDeAndrade_DISSERT.pdf: 1084420 bytes, checksum: fb713a41b783707222b94cd689206a20 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-06-29T17:02:00Z (GMT) No. of bitstreams: 1 KivyaDiasDeAndrade_DISSERT.pdf: 1084420 bytes, checksum: fb713a41b783707222b94cd689206a20 (MD5) / Made available in DSpace on 2016-06-29T17:02:00Z (GMT). No. of bitstreams: 1 KivyaDiasDeAndrade_DISSERT.pdf: 1084420 bytes, checksum: fb713a41b783707222b94cd689206a20 (MD5) Previous issue date: 2015-03-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES) / O leite bubalino apresenta uma melhor qualidade microbiol?gica e menor contagem de c?lulas som?ticas (CCS). A adequa??o nutricional de minerais pode ser utilizada como estrat?gia para melhorar a defesa da gl?ndula mam?ria. O sel?nio (Se) por seu potencial antioxidante h? uma a??o efetiva sobre a diminui??o da CCS, al?m de funcionar como refor?o imune tamb?m na sa?de humana. Sendo assim, este trabalho teve como objetivo avaliar o efeito da dieta com diferentes n?veis de sel?nio sobre a composi??o f?sico-qu?mico e da contagem de c?lulas som?ticas do leite de b?falas, assim como verificar o res?duo de sel?nio no leite e do queijo Minas Frescal. Para a an?lise da composi??o f?sico-qu?mico e da CCS foram utilizados 2264 dados de b?falas da ra?a Murrah, pertencentes a empresa Tapuio Ltda, localizada na regi?o agreste do estado do Rio Grande do Norte, no per?odo de 2010 a 2014. Para verificar a quantidade do res?duo de sel?nio presente no leite bubalino e no queijo minas frescal, foram utilizadas 100 b?falas Murrah divididas em 5 lotes. Os lotes foram formados de acordo com os n?veis de produ??o dos animais, onde a quantidade do concentrado fornecido v?ria dentro de cada lote, onde receberam 0,08 ppm/Se/kg de concentrado. Coletou-se 300 mL de leite do tanque e de cada lote, assim como 300 gr de queijo Minas Frescal, no per?odo de Agosto ? Novembro de 2014, sendo a coleta dos lotes realizada apenas no m?s de Novembro. As amostras foram enviadas para o Instituto de Tecnologia de Pernambuco para a an?lise de res?duo de sel?nio. As an?lises estat?sticas foram realizadas utilizando-se os procedimentos dispon?veis no pacote computacional SAS? (SAS, 2002). A CCS e os constituintes do leite apresentaram uma redu??o ? medida que ocorreu o aumento do uso do Se, no entanto como a produ??o de leite tamb?m aumentou, estes fatores tamb?m podem estar ligados ao fator dilui??o. N?o foi observado nenhum res?duo de sel?nio nas amostras do leite dos lotes, tanque e do queijo minas frescal. / This study aimed to evaluate the effect of diet with selenium on the physico-chemical composition and somatic cell count of buffalo milk, and check the selenium waste in milk and minas kind fresh cheese. For the analysis of physical-chemical composition and SCC were used 2264 buffalo milk data from the Murrah, belonging to Tapuio Ltda, located in the agreste region of the state of Rio Grande do Norte, in the period 2010 to 2014. To check the amount of selenium residue in buffalo milk and fresh cheese mines were used 100 Murrah buffaloes divided into 5 lots. The batches were formed according to the production levels of animals, wherein the amount of concentrate fed varied within each lot, which received 0.08 ppm / Se / kg of concentrate. Was collected from 300 ml of tank milk from and each lot, as well as 300 gr minas cheese, from August to November 2014, with the collection of lots held only in the month of November. The samples were sent to the Pernambuco Tecnology Institute for selenium residue analysis. The effect of the seasons was contrasted in two ways, namely: Spring, Summer, Autumn and Winter season or drought and rainy season. The analyzes statistics were performed using the procedures available in the software SAS? (SAS, 2002). Selenium level provided to the animals in this study was not significant to the point of detection of their residue in milk and the cheeses. However, it influences the reduction of the CCS. The milk characteristics were not affected by selenium use only the seasons.

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2016-07-11T17:13:30Z No. of bitstreams: 1 EduardoAlonsoCruzMonroy_DISSERT.pdf: 2026837 bytes, checksum: 07d08221eedeeea6100a427aaf6ca1d9 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2016-07-14T17:30:27Z (GMT) No. of bitstreams: 1 EduardoAlonsoCruzMonroy_DISSERT.pdf: 2026837 bytes, checksum: 07d08221eedeeea6100a427aaf6ca1d9 (MD5) / Made available in DSpace on 2016-07-14T17:30:27Z (GMT). No. of bitstreams: 1 EduardoAlonsoCruzMonroy_DISSERT.pdf: 2026837 bytes, checksum: 07d08221eedeeea6100a427aaf6ca1d9 (MD5) Previous issue date: 2016-02-26 / Les?es odontog?nicas epiteliais benignas s?o entidades de grande import?ncia cl?nica que se desenvolvem nos ossos maxilares a partir dos tecidos que formam os dentes. Tem sido demonstrado que em tumores benignos e malignos, est?o presentes um grande n?mero de c?lulas tronco tumorais, as quais tem grandes implica??es no desenvolvimento dos tumores. Oct-4 e CD44 t?m sido demostrados como importantes marcadores para c?lulas-tronco tumorais. O objetivo deste estudo foi identificar c?lulas epiteliais que expressam marcadores de c?lulas tronco atrav?s da express?o imuno-histoqu?mica de Oct-4 e CD44 em uma s?rie de casos de les?es odontog?nicas epiteliais ben?gnas. A amostra foi constitu?da por 20 casos de ceratocisto odontog?nico (CCO), 20 caos de Ameloblastoma s?lido/multic?stico e 20 casos de Tumor Odontog?nico Adenomatoide (TOA). A express?o de Oct-4 e CD44 foi avaliada no epit?lio das les?es atrav?s do percentual de c?lulas positivas(PP) e da intensidade da express?o ( IE ), sendo realizado o somat?rio destes escores, resultando na Pontua??o de Imunomarca??o Total (PIT) que variou de 0 a 7. Os resultados do presente estudo foram analisados pelo valor da pontua??o de PIT. Todos os casos apresentaram positividade para os dois marcadores e a maioria exibiu alta express?o para ambos os marcadores. A an?lise da express?o de Oct-4 n?o revelou diferen?as estatisticamente significativas (p = 0,406) entre as les?es estudadas. Com rela??o ? express?o do CD44, houve diferen?a estatisticamente significativa entre os casos de ameloblastoma e CCO, apresentando este ?ltimo maior n?mero de casos no score 7 (p = 0,034). Na analise da correla??o da imunoexpress?o de ambos os marcadores nas tr?s les?es estudadas, n?o houve correla??o estatisticamente significativa . Os resultados do presente estudo identificaram a presen?a de c?lulas com caracter?sticas troncais dispostas em locais variados do componente epitelial das les?es ora estudadas sugerindo a sua poss?vel participa??o na histog?nese e diferencia??o em les?es odontog?nicas epiteliais benignas contribuindo assim para o desenvolvimento destas les?es. / benign epithelial odontogenic lesions are great clinical importance entities that develop in the jaws from the tissues that form teeth. It has been shown that in benign and malignant tumors, are present in a large number of tumor stem cells, which has great implications in the development of these lesions. Oct-4 and CD44 have been demos as important markers for tumoral stem cells. The objective of this study was to identify epithelial cells expressing stem cell markers by immunohistochemical expression of Oct-4 and CD44 in a series of cases of benign epithelial odontogenic lesions. The sample was comprised of 20 cases of odontogenic keratocyst (OKC), 20 cases of solid/multicystic ameloblastoma and 20 cases of adenomatoid odontogenic tumor (AOT). The expression of Oct-4 and CD44 was evaluated in epithelial lesions using the percentage of positive cells (PP) and the intensity of expression (IE), being realized the sum of these scores, resulting in Total Immunostaining Score (TIS) ranging 0 to 7. The results were submitted to the appropriate statistical test (nonparametric Kruskal-Wallis and Spearman correlation coefficient). All cases were positive for both markers and most showed high expression of both markers. The analysis of Oct-4 expression revealed no statistically significant differences (p = 0.406) among the studied lesions. Regarding the CD44 expression, there was a statistically significant difference between the cases of ameloblastoma and TOA in relation to the CCO, with the latter show more cases in the score 7 (p = 0.034). In the correlation analysis of the immunoreactivity of both markers in the three lesions studied, there was no statistically significant correlation. The results of this study identified the presence of cells with stemness characteristics arranged at various sites in the epithelial component of the studied lesions suggesting their possible role in the histogenesis and differentiation in benign epithelial odontogenic lesions, thus contributing to the development of these lesions.