Neste trabalho foram investigados os mecanismos e o perfil cinético de processos nitrosativos do ponto de vista da nitrosação do indicador 4,5-diamino fluoresceina (DAF2) em células do tipo RAW 264.7. Também foi investigado o papel que ferro lábil (LIP) exerce em tais processos. O estudo cinético mostrou que a nitrosação do DAF2 é dependente de superóxido intracelular e se processa por dois mecanismos distintos denominados nitrosilação oxidativa e nitrosação. Observou-se que o perfil cinético da nitrosaçao do DAF2 sofre uma transição passando de dependente para independente com relação à concentração de NO, quando a concentração de NO se aproxima de 100-110nM. Este perfil está relacionado com a dinâmica de recombinação entre NO e O2¯ que dispara todo o processo de nitrosação do DAF2. No trabalho fica claro que processos nitrosativos que ocorrem pelos mesmos mecanismos podem apresentar perfis cinéticos completamente diferentes dependendo da localização onde ocorre a recombinação entre NO e O2¯. O ponto mais interessante foi a constatação de que quelantes permeáveis à membranas biológicas estimulam a nitrosação do DAF2 intracelular. Este efeito é decorrente da remoção de LIP intracelular que, surpreendementemente, apresenta papel antinitrosativo nas condições experimentais estudadas. O papel incomum antinitrosativo apresentado por LIP é analizado do ponto de vista da reação entre LIP e ONOO¯ que tem como produto nitrito, uma espécie não nitrosante. Estes resultados podem alterar a forma como LIP é visto em processos oxidativos e nitrosativos. / In this work, we investigated the mechanisms and kinetic profiles of nitrosative processes using fluorescent indicator 4,5-diaminofluorescein (DAF2) in RAW 264.7 cells. The labile iron pool (LIP) influence in nitrosative processes was also evaluated. Intracellular DAF2 nitrosation is superoxide dependent and proceeds by two distinct mechanisms: Oxidative nitrosylation and nitrosation. The former mechanism is the most relevant under all experimental conditions tested. Interestingly, the DAF2 nitrosation rate increases linearly with NO concentration of up 100-110 nM but thereafter undergoes a sharp transition and becomes insensitive to NO. This peculiar kinetic behavior has never been reported and it is linked with NO and superoxide recombination dynamics. When NO reaches a concentration capable to outcompete superoxide dismutase for superoxide, the rate of DAF2 nitrosation becomes insensitive to NO. The most striking finding is the LIP´s influence in nitrosative processes. LIP removal by cell membrane permeable metal chelantors increases DAF2 nitrosation rate significantly, suggesting tha LIP can act as an anti-nitrosant species. This increase is probably related with LIP´s direct reaction with peroxynitrite, wich produces non-nitrosant species like nitrite. This controversial LIP´s anti-nitrosative role in cellular systems is rather interesting since it can change the way we understand it´s role in nitrosative and oxidative processes.

O meio ambiente se apresenta como um dos desafios da governança global no que se refere às abordagens de Relações Internacionais e Ciência Política. Isso se deve ao fato de que os recursos naturais não se submetem à soberania direta do Estado e/ou das organizações internacionais formais como fonte de autoridade devido à transnacionalidade que o tema enseja. Neste sentido, os diferentes atores das relações internacionais, estatais e não estatais, precisam construir arenas de atuação, criar regulamentações onde os Estados (eventualmente) não estão presentes, e criar instrumentos de enforcement e compliance. No entanto, a centralidade das questões ambientais é colocada em xeque por teorias racionalistas de relações internacionais baseadas na autoridade do Estado e de sua capacidade de enforcement top-down. O meio ambiente é um assunto melhor considerado por arenas transnacionais em um contexto multinível e policêntrico. Neste sentido, a análise em nível local e a capacidade de organização de grupos sociais na constituição dos arranjos institucionais através da ação coletiva para solucionar a possível \"tragédia dos comuns\" tem atraído estudiosos que procuram demonstrar a sua efetividade e, consequentemente, a sua contribuição para a resolução das contendas ambientais globais. Desta forma, o objetivo deste trabalho é demonstrar como a relação entre a governança dos recursos de uso comum em âmbito local vincula-se aos instrumentos de governança global definidos por governos e organizações internacionais formais para o uso da biodiversidade. Inicialmente, realiza-se revisão bibliográfica da literatura de Relações Internacionais e Ciência Política para articular as questões ambientais entre o local e o global para, posteriormente, revelar como a governança ambiental multinível e policêntrica é estabelecida para o caso do uso da biodiversidade em comunidades locais na Amazônia brasileira, como apresentado no trabalho empírico. / The environment is presented as one of the challenges of global governance with regard to the approaches of International Relations and Political Science. This is due to the fact that natural resources are not subjected to the direct sovereignty of the State and/or formal international organizations as a source of authority because of the transnationality that the subject entails. In this sense, the different actors of International Relations, State and non-State, need to build action arenas, create regulations where States (eventually) are not present, and create instruments of enforcement and compliance. However, the centrality of environmental issues is kept in check by rationalist theories of International Relations based on States\' authority and ability to top-down enforcement. The environment is a subject better considered in transnational arenas in a multilevel and polycentric context. In this sense, the analysis at the local level and the organizational ability of social groups in the constitution of institutional arrangements through collective action to address the possible \"tragedy of the commons\" has attracted scholars seeking to demonstrate its effectiveness and, consequently, their contribution for the resolution of global environmental issues. Thus, the aim of this study is to demonstrate how the relationship between the governance of the common-pool resources at the local level is linked to the global governance instruments set by governments and formal international organizations for the use of biodiversity. Initially, a literature review of International Relations and Political Science literature is carried out to articulate environmental issues between the local and the global to reveal how the multilevel and polycentric environmental governance is established in the case of the use of biodiversity in local communities in the Brazilian Amazon, as shown in the empirical work.

Este estudo trata da informação que permeia os contratos e de sua aplicação ao contrato de seguro, da perspectiva do Direito e da Economia, ciências sociais que se complementam, porque aplicadas à mesma realidade, que será mais fielmente retratada se analisada sob ângulos diferentes, mas correlatos. Perquirindo a função da informação no contrato, constata que, se concernente a elemento essencial deste, a ele adere, passando a integrá-lo, o que determina sua importância nesse contexto e indica o regime jurídico que lhe deve ser aplicado. A investigação da distribuição da informação entre os contratantes e dos efeitos eventualmente nocivos da assimetria informacional, como o incentivo ao oportunismo, o aumento dos custos de transação e a obtenção de ganhos indevidos do contrato, induz à discussão dos critérios orientadores da disciplina jurídica da informação no âmbito contratual. A despeito da utilidade dos padrões para disciplinar condutas não alcançadas pelas regras, este estudo aponta que a boa-fé, em razão de suas idiossincrasias, não é padrão eficiente para reger a informação nos contratos, devendo ficar relegada à função residual. A aplicação do dever de informar com o objetivo de impor às partes transparência e veracidade conferiria mais objetividade e operacionalidade ao regime da informação nos contratos. Mas, a despeito da questionável eficiência da boa-fé como indutora da troca de informações entre as partes, foi o padrão de conduta escolhido pelo sistema jurídico para balizar a interação dos contratantes. Devido às peculiaridades do contrato de seguro, e à nocividade dos efeitos da assimetria informacional neste contexto, exige-se dos contratantes a máxima boa-fé. Se a regra é a máxima transparência e a absoluta veracidade, deverá ser restritiva a interpretação de eventuais exceções. Como a informação se prende ao cerne da operação econômica subjacente, afetando o cálculo do risco e a fixação do prêmio, e consequentemente, a mutualidade, diz respeito à função e à finalidade do instituto. Por isso, a interpretação condescendente de eventuais omissões ou distorções de informação relevante afrontaria o princípio da máxima boa-fé, que não pode ser mitigado, sob pena de comprometer o equilíbrio do contrato e afetar sua finalidade sócio-econômica. O estudo demonstrou a inadequação do tratamento da informação em relação ao substrato econômico do contrato de seguro, especialmente no que concerne à exigência de comprovação da má-fé nas omissões e distorções da verdade pelo segurado. Criticou também a aplicação dogmática da presunção da boa-fé, que reverte ao segurador o ônus da prova da má-fé do segurado, anulando o efeito sancionador da imposição do dever de informar. / The purpose of this dissertation is to analyze the importance of information in contract law, the disclosure duties and its application to insurance contracts, from legal and economic perspectives. Since Law and Economics are social sciences applied to the same environment and are mutually complementary, this bifocal approach leads to a more accurate portrait of reality seen from different but correlated points of view. The analysis of the role of information reveals that if it concerns the contract essential element, it becomes part of it and determines the legal rules that should be applied to it. The inquiry of information distribution patterns shows that it can eventually bring about detrimental effects which induce the discussion of the criteria underlying the legal regime of information in contract law. Asymmetric information can be harmful if it encourages opportunism, increases transaction costs and grants one party undue gains from the contract. In spite of the usefulness of standards to regulate conducts not reached by rules, this study shows that good faith, due to its idiosyncrasies, is not an efficient standard to govern information in contracts. Thus, it should be assigned a residual function. The application of the duty to inform with the purpose of imposing full disclosure and accuracy to the parties ensures more objectivity to the information regime in contracts. However, good faith was the standard chosen by the legal system to rule the parties interaction, despite its recognized inefficiency to induce information exchange among agents. Due to the particular features of the insurance contract, and to the harmful effects of informational asymmetry in this context, law imposes the parties a higher standard of good faith. If the legal standard is the utmost good faith, eventual exceptions to this pattern should be restrictively interpreted. Since information is connected with the economic mechanism of the insurance contract because it affects risk and premium evaluation, it is strictly related to the function and purpose of the contract. Therefore, condescending interpretation of nondisclosure, misrepresentation and fraud would violate the principle of utmost good faith. Its mitigation will affect the contract balance and its economic and social purposes. This dissertation demonstrates the inadequacy of the information legal regime, especially regarding the requirement of proving bad faith related to nondisclosure, misrepresentation or fraud. It also criticizes the dogmatic application of the presumption of good faith that lays upon the insurer the burden of proving bad faith of the insureds conduct, nullifying the sanctioning effect of the imposition of the duty to inform.

Magister Philosophiae - MPhil (LAS) (Land and Agrarian Studies) / Government's decision to devolve irrigation management to farmers was partly influenced by international policy imperatives, which were propounded mainly by institutions associated with the Consultative Group for International Agricultural Research (CGIAR) and the inability by the government to continue funding operation and maintenance costs. The central question of the study is to understand the effects of Irrigation Management Transfer (IMT) on productivity, operation and maintenance in the Mamina Irrigation Scheme. Interviews with various primary and secondary stakeholders that included the irrigators, local political leadership and locally-based agriculture extension officers were carried out. The interviews were aimed at getting an insight on land tenure, participation and representation of women, water and electricity supply system and pricing, effects of irrigation management arrangements on equity and productivity, understanding the irrigators' food security status, operation and maintenance arrangement after Irrigation Management Transfer (IMT). Findings of this study suggest that the existing governance arrangements have partly led to low crop productivity, increased water and electricity bill arrears, poor water distribution, change to uneconomic plot sizes, unsustainable increase in the number of irrigators, failure to organise for operation and maintenance. The key factors influencing the poor performance include poor collaboration, pumping system that utilised more electricity, inability of the irrigators to replace leaky pipes, failure of the irrigators to contribute towards electricity and water bills, failure of the irrigators to contribute towards operation and maintenance. The study identified nine challenges that affected the success of IMT. The challenges that lay at the heart of Mamina irrigation scheme were mainly caused by the poor irrigation technology design, pricing structure of electricity, water permit system, inequalities in water distribution, low gender participation and representation, non-availability of formal markets for certain crops, food insecurity, plot alloction and land disputes. Poverty analysis has shown that the irrigators' ability to escape from poverty or food insecurity is critically dependent upon their access to assets. Different assets are required to achieve different livelihood outcomes. The cycle of accumulation of utility bill arrears continued even after devolution because the same defective irrigation infrastructure was transferred to the irrigators. In the case of Mamina irrigation scheme, modernisation of the scheme was required to achieve different livelihood outcomes, but because this did not happen the recurrent utility bill arrears, low productivity and food insecurity continued to be a very serious challenge even after IMT.

O presente trabalho buscou reproduzir, a partir de simulação computacional utilizando modelagem baseada em agentes, a dinâmica do sistema sócio-ecológico que conecta comunidades quilombolas do Vale do Ribeira, São Paulo, ao palmito jussara (Euterpe edulis Martius), espécie considerada ameaçada de extinção e cuja exploração, atualmente, pode ser descrita como uma Tragédia dos Comuns. Com base na teoria acumulada de recursos comuns, em dados empíricos do sistema social quilombola e na dinâmica populacional do palmito, simulamos o impacto de duas políticas públicas distintas de gestão do recurso: a primeira é a proibição estatal de exploração do mesmo, que criminaliza os palmiteiros e não está surtindo os efeitos desejados de preservação; a segunda, projeta um cenário hipotético no qual o Estado flexibiliza as regras de exploração e permite que a comunidade gerencie o recurso. Ao comparar o estoque final de palmito jussara para diversos cenários, concluimos que existe um grande potencial para que a gestão comunitária do recurso traga benefícios econômicos às famílias quilombolas, ao mesmo tempo em que contribui para a preservação do mesmo na natureza. / This study intended to use agent-based modeling to reproduce the dynamics of the social-ecological system that connects the quilombola communities that live in the Ribeira Valley, São Paulo, to the palm tree Euterpe edulis Martius, an endangered species highly valued for its heart of palm, which has being heavily harvested and can be considered an example of a Tragedy of the Commons. Based on the theory on common-pool resources, empirical data regarding the quilombola social system and on the palm tree population dynamics, we simulate the impact of two different public policies towards the palm tree conservation: the first scenario is the current total-prohibition of extraction policy, which marginalize harvesters and is not working prom an environmental perspective; the second scenario simulates a hypothetical situation in which allows the community to explore and manage the resource. After comparing the final stock of Euterpe edulis for many conditions, we may conclude that there is a great possibility that community-level management is more able to bring economic benefits to the quilombola families, while also help to preserve the species.

Submitted by Kelson Anthony de Menezes (kelson@ucb.br) on 2016-12-09T12:13:15Z No. of bitstreams: 1 FernandaHellenadeLimaQueirozDissertacao2015.pdf: 1995292 bytes, checksum: 48b0df6bfef7ed36506303aa5b3a0dc8 (MD5) / Made available in DSpace on 2016-12-09T12:13:15Z (GMT). No. of bitstreams: 1 FernandaHellenadeLimaQueirozDissertacao2015.pdf: 1995292 bytes, checksum: 48b0df6bfef7ed36506303aa5b3a0dc8 (MD5) Previous issue date: 2015-12-16 / This dissertation presents regional economic co-operation as a facilitator for the development of energy governance strategies, focusing Brazil???s regional insertion in the continent???s Southern cone. The main goal of this Masters level dissertation is to analyse the practices made by Nordic???s model of electricity production & commercialisation (Nord Pool), establishing a paradigm between Brazil???s model in force and the interaction possibilities with Brazil???s neighbouring countries. The feasible strategies for the creation of optimal environments, the influence of economic co-operation in the negotiations carried on by South American countries and the guidelines of energy governance will also be analysed for the growth at the electricity supply levels (energy security), regional development and social welfare. / Esta disserta????o apresentou o tema da coopera????o econ??mica regional como facilitador para a elabora????o das estrat??gias de governan??a energ??tica que visa ?? inser????o regional do Brasil no Cone Sul. Teve-se por objetivo estudar as pr??ticas do modelo n??rdico de produ????o e comercializa????o de energia el??trica, denominado Nord Pool, na tentativa de ser estabelecida uma alternativa para o desenvolvimento do setor el??trico brasileiro que se encontra em estado cr??tico. De fato, o Brasil n??o ?? autossuficiente no que diz respeito ?? produ????o energia el??trica. Foram comentados os acordos eletroenerg??ticos j?? existentes e as possibilidades de integra????o regional com os pa??ses cont??guos ao Brasil, que permitem a concilia????o do desenvolvimento econ??mico com a preserva????o dos recursos naturais, por meio dos instrumentos de pol??tica governamental. Por isso, foi estabelecido um link entre a coopera????o econ??mica internacional, abordando as tratativas realizadas entre os pa??ses que possuem interesses comuns, e as diretrizes de governan??a energ??tica para o aumento da oferta de energia (seguran??a energ??tica), do desenvolvimento regional e do incremento do bem-estar social.

This dissertation examines the factors influencing a state’s decision to adopt a local government investment pool (LGIP), the institutional arrangements used in managing them, and the effect of the institutional types on LGIP performance. The dissertation extends the policy adoption theory with insights from investment theory to demonstrate that management credibility influences the likelihood of a state’s LGIP adoption. The study finds that the quality of financial management, the quality of professional leadership, proximate state neighbors who have previously adopted an LGIP, and economic factors determine a state’s proclivity to policy adoption. The dissertation also describes the institutional arrangements used in managing LGIPs and develops typologies based on their institutional arrangements. The dissertation compares LGIPs depending on the risk aversion of their institutional arrangements. The research extends the risk-return tradeoff in investment theory to LGIP institutional arrangements. The empirical findings show that LGIP institutional arrangement that has greater risk report higher performance. The dissertation also finds that competition in the LGIP market due to multiple vendors, and periods of economic recession account for higher performance because of higher risk-taking behaviors associated with them. This dissertation promotes public funds investment laws that emphasize prudent management of government finances and guides managers of the public purse on the types of institutional choices that optimize returns with minimal risk.

Cette thèse examine comment répondent des consommateurs aux mécanismes visant à réduire leur consommation d'énergie. Ce besoin de réduction découle de la nécessité d'atteindre les objectifs de réduction d'émissions de gaz à effet de serre, d'augmenter la production d'énergie à partir d'énergie renouvelables et de réaliser des économies d'énergie. Ces objectifs exigent que la demande résidentielle soit plus flexible face à l'évolution de l'offre et que des économies d'énergie soient réalisées par les ménages. Le premier chapitre explore les barrières à l'acceptation et à l'adoption des compteurs intelligents et des incitations qu'ils peuvent fournir. D'importantes barrières existent et les réductions de consommation sont loin d'être réalisées. Le manque de motivation, l'incompréhension de l'information sur la consommation et la rigidité de la vie quotidienne sont les principales barrières qui limitent la réponse des ménages aux incitations fournies par les compteurs intelligents. Le deuxième chapitre analyse les résultats d'expériences de terrain et d'études pilotes portant sur les impacts des différentes incitations sur la consommation résidentielle. Les résultats montrent qu'il existe de grandes variations et qu'en moyenne, une incitation entraînera une réduction de 2% de la consommation d'énergie. Les incitations de feedback en temps réel ainsi que l'information monétaire ont le plus grand effet. Enfin, les études plus robustes font état d'effets de réduction plus faibles. Dans le troisième chapitre, un jeu expérimental de ressources communes est utilisé pour explorer les réponses individuelles aux incitations basées sur le prix et les nudges. Les individus sont encouragés à réduire leur consommation, soit par une augmentation de prix, soit par des smiley évocant leur surconsommation. Le prix est le plus efficace pour encourager le niveau cible de consommation, mais il faut plus de temps pour qu'il fasse effet. Le nudge est compris rapidement mais tend à renforcer les comportements de surconsommation. Le quatrième chapitre examine l'effet du framing sur la disposition à l'effort. Les individus doivent accomplir une tâche simple et répétitive pour laquelle ils reçoivent un paiement à la pièce sous forme d'un gain ou d'une perte. Le framing sous forme de gains et de pertes est combiné à trois structures de paiement différentes : gain fixe, gain faible ou élevé avec une probabilité égale révélée avant ou après la réalisation de l'effort. Les résultats montrent que le framing n'a aucun effet sur la réalisation de l'effort, excepté pour un contexte de gain élevé annoncé avant de fournir l'effort. / This thesis examines how consumers respond to incentives used to encourage a reduction in their energy consumption. This necessary reduction stems from the need to reduce greenhouse gas emissions, increase energy production from renewable energy sources and achieve energy savings. These objectives require that residential demand be more flexible in response to changes in supply and that energy savings be achieved by households. The first chapter explores the barriers to consumer acceptance and adoption of smart meters and the incentives that they provide. Significant barriers exist and consumption reductions are far from being achieved. Limited motivation, lack of understanding of information on consumption and the rigidity of daily life are the main barriers preventing households from acting upon the incentives delivered via smart meters. The second chapter analyses the results of field experiments and pilot studies on the impacts of different incentives on residential consumption. The results show that there are large variations and that, on average, an incentive will result in a 2% reduction in energy consumption. Real-time feedback and monetary information have the greatest effect. Finally, more robust studies report lower reduction effects. In the third chapter, a common pool resource game is used to explore individual responses to price and nudge-based incentives. Individuals are encouraged to reduce their consumption either by price increases or by smilies that reflect their overconsumption. The price is most effective at encouraging the target level of consumption but takes longer to have an effect. The nudge is quickly understood but tends to reinforce overconsumption behaviours. The fourth chapter examines the effect of framing on effort provision. Individuals are asked to complete a simple and repetitive task for which they receive a piece-rate payoff in the form of a gain or loss. Framing in the form of gains and losses is combined with three different payment structures: fixed gain, low gain or high gain with an equal probability revealed before or after the effort is made. The results show that framing has no effect on effort provision, except for a high gain context announced before making the effort.

Le Pacifique équatorial est une région clé pour l'étude du phénomène climatique El Niño Oscillation Australe (ENSO) qui joue un rôle essentiel sur la variabilité du climat à l'échelle globale. L'objectif de cette thèse est d'étudier, d'une part le rôle des ondes équatoriales sur le mécanisme de Recharge-Décharge d'ENSO et d'autre part, d'observer et comprendre l'impact des couches barrières de sel susceptibles d'influencer ENSO. Le mécanisme de Recharge-Décharge de la bande équatoriale (5°N-5°S) est analysé sur la période 1992-2006 à l'aide de données altimétriques. Les variations de volume d'eau chaude qui rechargent ou déchargent la bande équatoriale sont bien représentées par les transports méridiens de masse exprimés en terme de transports géostrophiques et d'Ekman. Les ondes équatoriales de Rossby premier mode méridien premier mode barocline jouent un rôle important sur la partie géostrophique de ces transports. Une classification des évènements El Niño est également effectuée à l'aide des variations de volume d'eau chaude du Pacifique équatorial. La variabilité de la structure thermohaline de la warm pool est analysée dans la bande équatoriale, à l'aide de données Argo, sur la période 2000-2007. Une validation de ces données ainsi que des tests de différents critères d'estimation des couches barrières de sel sont présentés. Les co-variabilités entre les couches barrières de sel, le front de salinité, les températures de surface et la hauteur dynamique sont analysées. Elles montrent notamment que les températures de surface élevées (SST>28-29°C), associées au maximum de convection atmosphérique sont fortement liées à la présence de couches barrières de sel épaisses (>20m). Les couches barrières de sel sont favorisées par des fortes précipitations, de faibles vents, l'advection zonale de salinité de surface et le cisaillement vertical de courants. Pour la première fois à partir d'observations, l'influence des ondes équatoriales sur les couches barrières de sel est mise en évidenc

This dissertation contributes to the debate on Common Pool Natural Resources (CPNRs) through the analysis of a specific case study: rangeland in southwest agro-pastoral Niger. The management of CPNRs is the subject of a lively debate pertaining to the property rights regime that may accompany CPNR management. This dissertation shows that this debate may very well be the consequence of disciplinary preconceptions that are linked with disciplinary paradigms. By approaching the case study with an interdisciplinary approach, this dissertation shows how interdisciplinary research may contribute to the avoidance of “universalism” (i.e., one property rights regime fits all CPNRs) with regard to CPNR management. <br />The case study is approached by using three different conceptual frameworks in order to explore five research questions. A first framework, econometric modelling, is used to explore the following two questions: “what are the determinants of rangeland use in southwest agro-pastoral Niger?” and “what are the determinants of livestock mobility?” A second framework, event chronology analysis, is used to explore the following two questions: “what is the impact of rainfall shocks on rangeland property rights?” and “is the marginalization of the pastoral space avoidable and reversible?” A third framework, network analysis, allows for the exploration of: “do traditional local institutions have the capacity to play a role in the management of rangeland?” The analysis and discussion of these research questions point to the fact that community-level rangeland management in southwest agro-pastoral Niger is a definite possibility. Nevertheless, it will be possible only if livestock raising practices are strong in the two traditional producer groups: agriculturalists and pastoralists. Community-level management will, therefore, have to be rooted in the community that is constituted by the users of the rangeland.<br />Finally this dissertation concludes by stressing the results in terms of rangeland-management policy for Niger, in terms of the interdisciplinary analysis of CPNR, and in terms of conducting interdisciplinary research in general.