Submitted by JÚLIO HEBER SILVA (julioheber@yahoo.com.br) on 2017-02-09T19:11:09Z No. of bitstreams: 2 Tese - Renata Silva do Prado - 2014.pdf: 3442501 bytes, checksum: b2afce5d2fc3ecb9aad886103a6345fa (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-02-13T09:53:54Z (GMT) No. of bitstreams: 2 Tese - Renata Silva do Prado - 2014.pdf: 3442501 bytes, checksum: b2afce5d2fc3ecb9aad886103a6345fa (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2017-02-13T09:53:54Z (GMT). No. of bitstreams: 2 Tese - Renata Silva do Prado - 2014.pdf: 3442501 bytes, checksum: b2afce5d2fc3ecb9aad886103a6345fa (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2014-03-14 / Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPq / Dimorphic fungi of the genus Paracoccidioides spp. are etiologic agent of paracoccidioidomycosis (PCM), a human systemic mycosis with high incidence in Brazil. Due to the toxicity of existing drugs, the long treatment and resistant isolates, the search for new therapies has become relevant. In this study, we verified the activity argentilactone, extracted the essential oil of Hyptis ovalifolia and its derivatives against Paracoccidioides. The results showed a dose- dependent inhibition of argentilactone fungus in yeast cells, as well as during dimorphism. Inhibition of cell growth was higher than in the presence of acetate than glucose. Argentilactone also been shown to inhibit the native enzyme isocitrate lyase Paracoccidioides (PbICL) and recombinant (PbICLr). The greatest inhibition was observed in the presence of acetate, a condition in which the enzyme is demonstrably more active than glucose. In silico analysis showed that argentilactone binds to the catalytic site of PbICL. The micro-assays for PbICLr and malate synthase (PbMLSr) were standardized, allowing the search for inhibiting compounds on a large scale. A global analysis of Paracoccidioides proteomic profile in response to argentilactone allowed visualization of metabolic adaptation of the fungus to the compound. Important metabolic pathways were suppressed explaining the strong action of the compound on fungal growth and viability. In this study, alternative metabolic pathways adopted by the fungus were elucidated resulting in a better understanding of the mode of action of the compound. It was also evaluated the cytotoxicity and DNA damage caused by argentilactone in human cells, MRC5 and A549, concluding that it occurs in a dose-dependent manner. Thus, it is concluded that argentilactone is a candidate prototype antifungal for the treatment of PCM. / Os fungos dimórficos do gênero Paracoccidioides spp. são agentes etiológicos da paracoccidioidomicose (PCM), uma micose humana, sistêmica de alta incidência no Brasil. Devido à toxicidade das drogas existentes, o longo tempo de tratamento e o aparecimento de isolados resistentes, a busca por novas terapias tem adquirido relevância. Neste estudo, verificou-se a atividade de argentilactona, extraída do óleo essencial de Hyptis. ovalifolia, bem como seus derivados, contra Paracoccidioides. Os resultados mostraram uma inibição dose-dependente de argentilactona em células leveduriformes do fungo, bem como durante o dimorfismo. A inibição de crescimento celular foi maior em presença de acetato do que glicose. Também foi demonstrado que argentilactona inibe a enzima isocitrato liase de Paracoccidioides nativa (PbICL) e recombinante (PbICLr). A maior inibição de PbICL foi observada na presença de acetato, condição em que a enzima é comprovadamente mais ativa, do que em glicose. Análises in silico, que compreenderam dinâmica e docking molecular a partir da construção de modelo de homologia e análise das interações intermoleculares e de energia de solvatação, mostraram que argentilactona se liga ao sítio catalítico de PbICL. Os micro-ensaios para PbICLr e malato sintase (PbMLSr) foram padronizados, o que permitirá a busca por compostos inibidores em larga escala. A caracterização global do perfil proteômico de Paracoccidioides em resposta à argentilactona permitiu a visualização da adaptação metabólica do fungo ao composto. Vias metabólicas importantes foram reprimidas explicando a forte ação do composto sobre o crescimento do fungo e viabilidade. Neste estudo, as vias metabólicas alternativas adotadas pelo fungo foram elucidadas resultando em uma melhor compreensão do modo de ação do composto em estudo. Foi avaliada, também, a citotoxicidade e o dano ao DNA causado por argentilactona em células humanas, MRC5 e A549, concluindo-se que o mesmo ocorre de maneira dose-dependente. Assim, conclui-se que argentilactona é um candidato a antifúngico para tratamento da PCM.

Les encéphalopathies spongiformes subaigües transmissibles (ESST) sont des maladies neurodégénératives affectant l'Homme et les animaux, l'issue est toujours fatale. La détection dans le sang de l'agent pathogène responsable de l'infection (protéine prion pathologique)reste difficile à ce jour et l'identification de nouveaux biomarqueurs impliqués dans la physiopathologie des ESST constitue un projet ambitieux et risqué. Dans ce contexte, notre objectif est d'établir un profil protéique corrélé aux ESST. L'utilisation d'un modèle animalbien caractérisé : la tremblante naturelle du mouton, d'une technologie adaptée à l'analyse de profils protéiques : SELDI-TOF MS et d'un fluide biologique : le sérum, a constitué la base de nos travaux de recherche. Dans un premier temps, les protocoles expérimentaux ont été mis en place et optimisés. Puis, ils ont été évalués pour leur pertinence dans la discrimination de moutons pathologiques en phases précoce et tardive de la maladie versus des moutons contrôles par analyse des sérums fractionnés ou non. Des biomarqueurs potentiels de faibles poids moléculaires ont été sélectionnés à l'aide de la méthode statistique SAM et une signature protéique permettant un diagnostic précoce a été établie (87% de sensibilité et 90%de spécificité). Un des biomarqueurs a été identifié comme étant un fragment de la transthyrétine, puis son potentiel discriminant a été évalué en SELDI-TOF MS dans une étude cinétique de hamsters Syriens infectés par la scrapie, en western blot et par dosage ELISA.Finalement, une cohorte de validation constituée de moutons appelés « scrapie-free » a permis de valider les biomarqueurs les plus pertinents. / Transmissible spongiform encephalopathies (TSEs) are fatal neurodegenerative diseasesoccurring in animals and humans for which no ante-mortem diagnostic test in biological fluidsis available. In such pathologies, detection of the pathological form of the prion protein (i.e.,the causative factor) in blood is difficult. Identification of new biomarkers implicated in thepathway of prion infection is relevant. In this context, our objective was to find a proteicprofile correlated to TSEs. We used a well-known TSE model: scrapie in sheep breeding, amass spectrometry technology easy-to-use for proteic profiling: SELDI-TOF MS and abiological fluid: serum. First, experimental tools have been developed and optimized. Thesetools were evaluated for their discriminating potential of control sheep and animals with earlyor late phase scrapie using a large number of serum samples (fractionated or not). Then, usingthe SAM statistical method, potential low molecular weight biomarkers were selected. Amongthese biomarkers, a protein signature pattern was identified; it can discriminate between earlyphase scrapie and control sera (sensitivity of 87% and specificity of 90%). One of theseproteins was identified as a fragment of transthyretin and evaluated as a biomarker using aSELDI-TOF MS kinetic study of sera from scrapie infected Syrian hamsters. This biomarkerwas also confirmed by western blot analysis and ELISA quantitation. Finally, a cohort of freescrapiesheep permits to validate the diagnostic potential of the candidate biomarkers.

Hemogen (Hemgn) is reported as a tissue specific transcriptional regulator in testis as well as hematopoietic tissues. It is known that Hemgn positively regulates erythroid differentiation; however,the underlying molecular mechanism is not well understood. In the current study, using proteomic approach in combination with other molecular biology tools,we have attempted to decipher the role of Hemgn in differentiating Murine erythroblast leukemia (MEL) cells as a model system. Our study reveals that Hemgn predominantly interacts with transcriptional regulators, chromatin modifiers and histones. Furthermore, using Chromatin Immunoprecipitation and knockdown approach, we have demonstrated that Hemgn is recruited to the b-globin locus, which is known to be activated during erythroid differentiation. Based on the results,we speculate that Hemgn acts as a tissue specific histone chaperone that regulates transcription during erythroid differentiation.

Mass spectrometry has increasingly become the method of choice for the analysis of complex biological samples, including proteins and lipids. This thesis describes the development of MS-based analytical methodologies for the analysis of complex proteomic and lipidomic samples. Chapter 3 describes the development of microfluidic proteomic reactors, in the formats of SCX reactor, SCX 96-well plate reactor, and SAX reactor, for the enzymatic digestion of complex proteomic samples for subsequent LC-MS/MS analysis. These microfluidic proteomic reactors greatly simplified the enzymatic digestion of complex proteomic samples by combining multiple processing steps, such as rapid extraction and enrichment of proteins. Furthermore, chemical and enzymatic treatments of proteins were all performed in a few nanoliters effective volume, resulting in an increased protein digestion efficacy. After the protein digestion process, the resulting peptides were eluted in buffers that were compatible with HPLC-MS/MS analysis. In chapter 4, a methodology based on nano-HPLC-ESI-MS/MS for the analysis of PAF and LPC lipid species is described. In this method, lipid extracts from biological samples were separated by nano-flow HPLC prior to being introduced into a Q-TRAP 2000 mass spectrometer, where the lipid species of interest were detected using a precursor ion scan at m/z 184. Absolute quantitation of PAF family lipid species were performed with standard addition method, where 5 standard solutions containing 0.2-1 ng each of C16:0, C18:0 PAF and C16:0, C18:0 lyso-PAF were used in the experiment. Further, the spiking of identical amount of non-endogenous C13:0 LPC at time of extraction allow the relative comparisons of other LPC lipid species of interest between different samples. The developed methods were employed to analyze the changes of PAF and LPC lipid species in NGFdifferentiated PC12 cells, in the posterior/entorhinal cortex of AD patients and TgCRND8 transgenic mice, and over the course of 24 hour exposure of human hNT neurons to Aβ42 treatment, respectively, in comparison to controls. iii Chapter 5 describes the development of a novel shotgun lipidomic methodology for the determination of stereospecificity of diacyl glycerophospholipids including glycerophosphatidic acids (PA), glycerophosphoserines (PS), glycerophosphoglycerols (PG), glycerophosphoinositols(PI), and glycerophosphoethanolamines (PE), which can be conventionally ionized under negative ion mode. The stereospecificity of diacyl glycerophospholipids was determined based on the relative abundance of the lyso-form fragment ions, attributed to the neutral loss of fatty acyl moieties. The fragmentation patterns of a variety of diacyl glycerophospholipid standards were first fully examined over a wide range of collision energy. We observed that lyso-form fragment ions corresponding to the neutral loss of fatty acyl moieties attached to the sn2 position as free fatty acids ([M-Sn2]-) and as ketenes ([M-(Sn2-H2O)]-) exhibited consistently higher intensity than their counter part ions due to the neutral loss of fatty acyl moieties attached to the sn1 position ([M-Sn1]- and [M-(Sn1-H2O)]-). We then examined the product ion spectra of diacyl glycerophospholipids recorded from lipid extracts of rat hepatoma cells, where the stereospecific information of these lipids was conclusively determined.

Orientador: Marco Aurélio Zezzi Arruda / Tese (doutorado) - Universidade Estadual de Campinas, Instituto de Química / Made available in DSpace on 2018-08-26T13:29:45Z (GMT). No. of bitstreams: 1 LopesJunior_CiceroAlves_D.pdf: 12621993 bytes, checksum: d178a6c055fde0c86926a36f0e49390c (MD5) Previous issue date: 2014 / Resumo: A busca para melhor entender a participação de elementos químicos no metabolismo de um organismo exige uma abordagem integrada entre as áreas da ciência devido à elevada envolvida complexidade. Esta Tese compreende o estudo realizado integrando as abordagens iônomicas, metalômicas e proteômicas para avaliar possíveis alterações no metabolismo do girassol (Helianthus annuus L.), mediante o desenvolvimento das plantas sob diferentes condições de irrigação com cádmio. Inicialmente, o estudo revelou que o girassol apresenta elevada resistência à contaminação com cádmio, sendo possível inferir, pela primeira vez, que o nível fitotóxico deste elemento para o organismo estudado é ca. 2,3 mg g-1. Um estudo ionômico desenvolvido empregando a técnica de ICP-MS foi realizado para avaliar a distribuição de vários elementos nas diferentes partes das plantas, buscando obter correlações com os processos biológicos exibidos pelas plantas durante o plantio. O estudo mostrou que os girassóis acumulam cádmio preferencialmente nas raízes, no entanto, ca. 65 ± 7% do total captado é translocado para as partes aéreas, fato que resulta na diminuição do transporte de ferro, cobre, magnésio e manganês para as folhas das plantas, sugerindo que há competição entre os elementos. Assim, pôde-se correlacionar a falta de tais nutrientes à clorose foliar exibida pelas plantas do grupo Cd-superior. O emprego da técnica de 2D-DIGE combinada a técnica de nESI-Q-TOF, também apresenta destaque na pesquisa, complementando os dados obtidos no estudo ionômico, por meio da identificação de proteínas nas folhas dos girassóis. As sementes das plantas Cd-tratadas apresentaram teor de cádmio superior ao recomendado pela FAO e OMS (0,1 mg/kg), sendo consideradas impróprias para o consumo humano. O protocolo de digestão in vitro revelou que as maiores concentrações deste elemento estão nas frações bioacessíveis das sementes. Na avaliação dos extratos protéicos das sementes empregando a hifenação SEC-ICP-MS foi identificado que o cádmio interage com moléculas de massa moleculares variando entre 70 a 0,7 kDa. Por fim, o teste de germinação evidenciou que o nível de cádmio acumulado nas sementes interfere no seu desenvolvimento / Abstract: The search for better understand the involvement of chemical elements in the metabolism of an organism requires an integrated approach between the areas of science due to the high complexity involved. This Thesis comprises study integrating ionomic, metallomic and proteomic approaches to evaluate possible changes in the metabolism of sunflower (Helianthus annuus L.), through the development of plants under different irrigation conditions with cadmium. First, the study showed that the sunflower presents high resistance to cadmium contamination, being possible infer, the first time, that the phytotoxic level of this element to the plant especies is ca. 2.3 mg g-1. An ionomic study carried out using an ICP-MS, it was performed to evaluate the distribution of various elements in different plant parts, seeking to obtain correlations with biological processes exhibited by plants during planting. The study showed that cadmium sunflowers accumulate preferentially in roots, but ca. 65 ± 7% of the total absorbed was translocated to the aerial parts, which it has resulted in decreased transport of iron, copper, magnesium and manganese to the leaves, suggesting that there is competition between the elements. Thus, the lack of such nutrients correlated to leaf chlorosis exhibited by plants of Cd-higher group. The application of the technique of 2D-DIGE combined technique nESI-Q-TOF, also features highlighted in the research, supplementing the data obtained in the ionomic study through the identification of proteins in the leaves of sunflowers. The seeds of Cd-treated plants presented higher levels of cadmium than recommended by FAO and WHO (0.1 mg kg-1), being considered unsuitable for diet human. The protocol of in vitro digestion showed that higher concentrations of this element are on bioacessible fractions of the seed. In the assessment of protein extracts of the seeds using the SEC-ICP-MS coupled, it was identified that cadmium interacts with molecules of molecular mass ranging from 70 to 0.7 kDa. Finally, the germination test reaveled that the level of cadmium accumulated in seeds interfere in its development / Doutorado / Quimica Analitica / Doutor em Ciências

Orientador: Marco Aurelio Zezzi Arruda / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Quimica / Made available in DSpace on 2018-08-14T13:56:22Z (GMT). No. of bitstreams: 1 Brandao_AdilsonRoberto_M.pdf: 5742287 bytes, checksum: 7cb927fa13dbb5ee8680f0508247fe25 (MD5) Previous issue date: 2009 / Resumo: Neste trabalho de Dissertação, os organismos em estudo foram sementes de soja transgênica e não-transgênica, sendo que a soja geneticamente modificada é do tipo Roundup Ready® (RR), na qual a inserção de um gene tornou a planta resistente a herbicidas que possuem o glifosato como princípio ativo. Procurou-se avaliar o perfil proteomico, enzimático e metálico em sementes de soja a fim de contribuir com uma recente área do conhecimento, a Metalômica. Os resultados deste estudo indicam que não foi encontrada variação quanto ao total de proteínas entre as amostras e, também, que não houve variação quanto ao número total de spots detectados nos géis de ambas as amostras para as faixas de pH avaliadas (3-10 e 4-7) após a separação do conjunto de proteínas por 2D PAGE. Porém, por meio do estudo de análise de imagens dos géis verificou-se que 10 spots apresentaram variações significativas quanto a expressão de proteínas entre as sementes ( 90%). Deste conjunto, 08 proteínas puderam ser identificadas por meio da obtenção dos espectros de massa empregando MALDI-QTOF MS e busca em banco de dados. Aparentemente, não foi encontrada variações em termos de atividade enzimática das enzimas CAT, GR, SOD e APX, avaliadas em gel e por espectrofotometria, entre as amostras de soja transgênica e não-transgênica no estágio de semente, portanto, não verifica-se uma situação de extresse oxidativo neste estágio. O mapeamento das espécies metálicas foi feito por SR-XRF e ICP MS e, alguns elementos puderam ser detectados (Co, Nb, Cd, Se, V, La, Ce, Th, Ru, Zr e Hg). Apenas 02 spots proteicos das amostras de semente de soja transgênica apresentaram teor de V que pudesse ser quantificado, no entanto, devido a enorme variação de gel para gel e de variações que são intrínsecas de sistemas biológicos, aspectos mais conclusivos sobre suas quantificações ainda não foram possíveis. Também, não foi encontrado na literatura relatos sobre a possível presença deste elemento nas proteínas identificadas / Abstract: In this work, transgenic and non-transgenic soybean seeds were studied, being the Roundup Ready® (RR) type, the soybean genectically modified, to which the insertion of the gene make the plant resistent to herbicides that present the glifosate as active principle. The proteomic, enzimatic and metal profiles were evaluated in the soybean seeds to contribute to a new area, Metallomics. The results indicated that no variation was obtained related to the amount of total proteins between both samples as well as no variation related to the number of those detected spots in the gels in the pI range evaluated (3-10 and 4-7) after 2D PAGE. However, from the gel image analysis, 10 spots presented significant variation related to the protein expression between the seeds ( 90%). From this set, 08 proteins were analyzed by MALDI-QTOF-MS and the peptide sequency of each one identified in the protein data bank. Aparently, no variation when considering CAT, GR, SOD and APX in terms of enzimatic activity for transgenic and non-transgenic soybean seeds was achieved, when spectrophotometry and gel electrophoresis were used. Apparently, there is not the detection of the oxidative stress at this stage. The metallic species mapping was carried out by SR-XRF and ICP MS, and some elements were detected (Co, Nb, Cd, Se, V, La, Ce, Th, Ru, Zr and Hg). Vanadium was quantificated in 02 spots of transgenic soybean seeds only; however, due to the high gel to gel variation and those ones commonly achieved in biological systems, conclusive aspects about their quantifications were not still possible. There is no information in the literature about a possible presence of V in those identified proteins. / Mestrado / Quimica Analitica / Mestre em Química

L’epicocconone est un produit naturel tricyclique de la famille des azaphilones isolé en 2003 d’un champignon Epicoccum nigrum. Ce composé se lie de façon covalente aux amines, conduisant à la formation d’une énamine fluorescente. Cette réaction, réversible en fonction du pH, fait de ce composé un excellent marqueur de protéines pour la détection sur gels d’électrophorèse compatible avec une analyse par spectrométrie de masse. La synthèse d’analogues de l’épicocconone a été engagée au sein de notre laboratoire, en basant sur une étape clé d’oxydation désaromatisante. Une étude approfondie de cette réaction a permis de mettre en évidence une haute diastéréosélectivité en fonction des conditions opératoires.Après introduction d’un cycle acylfuranonique diversement fonctionnalisé, une série d’analogues de l’épicocconone a été obtenue permettant d’établir la relation structure fluorescence et évaluer l’utilisation de ces biomarqueurs en protéomique. / Epicocconone is a tricyclic natural product of azaphilone family, isolated from the fungus Epicoccum nigrum. This compound covalently binds to primary amines, leading to a protein linked conjugate which is strongly fluorescent. This reaction, reversible according to the pH, makes of this compound an excellent proteins dye compatible with an analysis by mass spectrometry. Synthesis of epicocconone’s analogues has been undertaken in our laboratory.This synthesis is based on a key oxidative dearomatization. A study of this reaction allowed us to shed light on a high diastereoselectivity according to reaction conditions. After introduction of functionalized acylfuranone ring, a library of epicocconone’s analogues was obtained allowing us to establish the structure-fluorescence relationship and to estimate the use of these biomarkers in proteomics.

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Made available in DSpace on 2014-08-20T13:32:46Z (GMT). No. of bitstreams: 1 tese_marcia_magalhaes_mata.pdf: 1998479 bytes, checksum: c18127c54931180d99c33eb9ceb106cd (MD5) Previous issue date: 2013-06-24 / L. monocytogenes is the etiologic agent of listeriosis, a severe food-borne disease. This pathogen has also variable ability to adhere to food-processing surfaces. Thus, the aims of this study was at first to evaluate the influence of the temperature (04-10-25-37°C) and time of incubation (24-48-168h) on the formation of attached cells by L. monocytogenes strains of diverse origins, serotypes and lineages using a colorimetric microtitre plate method. After this, comprehensive proteomics experiments using label-free 1D- liquid chromatography/tandem mass spectrometry (1D-LC/tandem MS) were performed to determine if the global proteomic responses of L. monocytogenes strains (Siliken and F2365) is altered markedly as attached cells compared to its planktonic state when growth media and temperature are the same. Our results showed that attached cells produced by different origins of L. monocytogenes did not change significantly when subjected to experimental conditions, unlike what was observed with attached cells produced by different serotypes and lineages of L. monocytogenes, which were clearly affected by environmental conditions.such as temperature and time of incubation. The ability of lineage II and serotype 1/2a and 1/2b to form large amount of attached cells when compared with the others in specific conditions indicates that risks from Listeria adherence must be taken seriously in sensitive food environments in order to find safer alternatives to prevent contamination and further dissemination of listeriosis. Only 8 proteins demonstrated substantial changes in common between both strains and temperatures in attached cells compared to their planktonic counterparts. They are: GroEL, DnaK, PtsH, PdxS, Pgi, RpsB, RpsD, and RpsP. Moreover, it was observed that the cell surface protein BapL abundance, though low, was not enhanced in attached cells suggesting its role in adherence could be a generalized contribution to the cell wall hydrophobicity. Interestingly, our experiment suggest that at 25°C the attached cells in both strains undergo flagella synthesis repression. Also, Sig B Regulon can be associate with an enhanced general stress response occurs in lineage II Strain (Siliken) but not in lineage I Strain (F2365) and could relate to the consequences of attachment. The temporal survey-based approach demonstrates clearly that high coverage represents a powerful means to investigate dynamic responses in L. monocytogenes from a functional genomics perspective / L. monocytogenes é o agente etiológico da listeriose, uma doença severa de origem alimentar. Esse patógeno também é capaz de se aderir a uma grande variedade de superfícies do processamento de alimentos. Sendo assim, os objetivos deste estudo foram primeiramente, avaliar a influência da temperatura (04-10-25 e 37°C) e tempo de incubação (24-48-168h) na formação de células aderidas de cepas de L. monocytogenes de diferentes origens, sorotipos e linhagens utilizando o método colorimétrico em placas de microtitulação. Após, experimentos de proteômica abrangentes que não utilizam marcadores, como a Cromatografia líquida de 1D/ espectrometria de massa em tandem (1D-LC/tandem MS) foram realizadas para determinar se o perfil proteico global de células planctônicas e de células aderidas de cepas de L. monocytogenes (Siliken e F2365) foi alterado significativamente quando os meios de crescimento e de temperatura de incubação foram os mesmos. A partir dos resultados obtidos verificou-se que as células aderidas formadas por L. monocytogenes de diferentes origens não sofreram alterações significativas quando submetidas às condições experimentais, diferentemente do que foi observado com as células aderidas formadas por L. monocytogenes de diferentes sorotipos e linhagens, as quais foram claramente afetadas pelas condições do ambiente. A habilidade da linhagem II e dos sorotipos 1/2a e 1/2b de formar grande quantidade de células aderidas quando comparadas com as demais, em condições específicas, indica alto risco de contaminação e disseminação da listeriose, bem como a sobrevivência e persistência deste micro-organismo no ambiente. Com base na análise proteômica, apenas oito proteínas demonstraram alterações substanciais em comum entre ambas as cepas e temperaturas em células aderidas comparadas com suas respectivas células planctônicas. São elas: GroEL, DnaK, PtsH, PdxS, Pgi, RpsB, RpsD, and RpsP. Verificou-se também que a abundância da proteína de superfície celular BapL, embora baixa, não foi aumentada em células aderidas sugerindo que o seu papel na adesão pode ser uma contribuição generalizada para a hidrofobicidade da parede celular. De forma muito interessante, nosso experimento sugere que células aderidas a 25°C por ambas as cepas levam a uma síntese de repressão flagelar. E ainda, Sig B Regulon pode estar associado com o aumento em geral da resposta ao estresse ocorrido na cepa de linhagem II (Siliken) mas não na cepa de linhagem I (F2365) o que pode estar relacionado com as consequências da adesão. A técnica utilizada no experimento demonstrou claramente que com alta abrangência é possível estudar proteomas bacterianos representando assim uma ferramenta poderosa para investigar respostas dinâmicas em L. monocytogenes através de uma perspectiva de genômica funcional.