When attempting to convey certain political or ideological agendas in literary texts maintaining specific writing strategies can work as a useful tool. From a feminist perspective the use of écriture feminine as a means of undermining patriarchy has been largely neglected as well as misunderstood by many feminists. However, as argued in this essay, écriture feminine is not only a useful tool for pursuing a feminist agenda, but is also something that needs to be discussed due to the many misunderstandings of it. Resting on the theoretical perspectives of Judith Butler, Hélène Cixous, Antonio Gramsci, Eve Kosofsky Sedgwick and Richard Slotkin this essay investigates Angela Carter’s short story “The Lady of the House of Love” in relation to écriture feminine by exploring how the text rejects patriarchy and its idea of the gender binary. In this short story Carter re-works the classic Sleeping Beauty fairy tale and provides us with a feminist’s version of it. The main thesis of this essay is therefore that Carter challenges the gender binary by de-victimizing “woman” and by engaging in a style of writing that overturns western culture’s definitions of “woman” Carter provides a version of Sleeping Beauty that radically differs from the hegemonic/patriarchal versions.

Vaginal photoplethysmography was utilized in combination with standardized sleep-recording procedures to investigate changes in vaginal pulse amplitude (VPA) during both waking and sleeping conditions in low- and high-arousability females (n = 10 per group), as classified by the Sexual Arousability Inventory. Based upon previous research, it was predicted that both groups would exhibit similar mean levels of VPA during waking exposure to erotic stimuli and during various stages of sleep. Despite hypothesized physiological similarities between groups, the low-arousability group was expected to subjectively report less arousal during the waking erotic conditions.

Each year liver cancer kills more than half a million people, making it the third leading cause of cancer-related death worldwide. Annual incidence continues to rise steadily, both domestically and globally, increasing the burden of this disease. Advancements in the ability to obtain detailed molecular profiles of tumors have led to the successful development of targeted therapies for a number of different cancers. Unfortunately, however, the molecular pathogenesis of liver cancer is poorly understood relative to many other types of malignancies. Thus, the identification of factors contributing to the development and progression of liver tumors is a major goal of current research. In pursuit of this goal, I have utilized the Sleeping Beauty (SB) transposon system as a tool for forward genetic mutagenesis screening in mice. The SB system recapitulates the kinetics of spontaneous tumor development in humans by providing a stepwise accumulation of mutations. Micro-evolutionary processes within a developing tumor lead to the selective expansion of cells harboring mutations that confer some kind of selective advantage. By identifying the most prevalent mutation events within a specific tumor type across a large number of independent samples, a list of genes implicated as being involved in tumorigenesis can be generated. Using this approach, the Dlk1-Dio3 imprinted domain was identified as a site of frequent mutation in SB-induced hepatocellular carcinomas (HCCs). I discovered that the mechanistic basis for recurrent selection of transposon insertion within this domain in liver tumors involved activated expression of Retrotransposon-like 1 (Rtl1). I also found that RTL1 activation is a common event in human HCC, suggesting that it could potentially be beneficial as a therapeutic target in a subset of patients. Etiological factors related to liver cancer development are varied, but are linked by the fact that each provides a chronic liver injury stimulus that promotes the development of hepatic fibrosis. In fact, ˜ 90% of human HCC occurs in this context, and yet the majority of mouse liver cancer models fail to account for this important environmental component of the disease. I have conducted a screen for genetic drivers of liver cancer in the presence or absence of hepatic fibrosis. Comparison of mutation profiles between fibrotic and non-fibrotic tumors revealed largely non-overlapping sets of candidate genes, indicative of a differential selective pressure for mutations depending on the fibrotic context of the liver. Driver mutations identified preferentially in the presence of liver fibrosis have a high likelihood of relevance to human disease, given the similarities in environmental context and kinetics of mutation acquisition. Consistent with this idea, multiple genes with well-established roles in human HCC were found to be preferentially mutated in SB-induced tumors developed in a fibrotic liver. Before a candidate cancer gene identified in an animal model system can have an impact on human disease, its proposed role in tumorigenesis must be validated. Existing techniques for validation of putative liver cancer genes suffer from significant limitations including high cost, low throughput, and a level of complexity that prohibits widespread utilization. I have contributed to the generation of a novel tool for in vivo validation of candidate genes that is not subject to these limitations. By combining elements of recombinant adenoviral vectors and the piggyBac transposition system, we have generated a highly flexible gene delivery system with significant advantages over existing techniques. The Ad-PB system has broad accessibility and applicability, making it a valuable tool for advancing efforts to improve cancer therapies.

This project is aimed at sleeping EEG signal, especially at searching of sleeping graphoelements and next at processing signal, witch this segmentation go before. Charakterization of sleeping graphoelements and problems with their classification are outlined here. Principle of two detection methods of k-komplex are explained and processed by Matlab with graphically representation of results. Results of automatic classification are compared with scoring of two experts.

Background: Previous studies have demonstrated that breastfed newborns spend more time awake than bottle‐fed newborns, breastfeeding mothers have more fragmented sleep than bottle‐feeding mothers, and mother‐newborn sleeping arrangements may affect the sleep/wake pattern of mother‐newborn pairs. Objective: To address the unsolved question of whether there is an interaction between type of feeding and sleeping arrangements that affects postpartum sleep during the 4th postpartum week. Design: Correlational, two‐way design using feeding method and location of newborn at night as independent variables, and sleep patterns as the dependent variables.Setting: Patient's home during 4th week after giving birth. Patients/Participants: First‐time mothers and their newborns (n = 33). Main Outcome Measures: Amount of total sleep, amount of night sleep, number of night awakenings, and number of sleep periods in 24 hours using a modified version of the self‐report sleep instrument by Barnard and Eyres. Results: Breastfed newborns had less total sleep per day than bottle‐fed newborns, and breastfeeding mothers had more sleep periods in 24 hours than bottle‐feeding mothers. Breastfeeding mothers slept more than bottle‐feeding mothers when co‐sleeping, but bottle‐feeding mothers’ sleep was unaffected by location of newborn. Average total sleep for 4‐week‐old newborns was about 14 hours daily. Conclusions: More sleep was obtained when breastfeeding mothers slept with the newborn. Methods or devices that allow breastfeeding mothers and newborns to sleep next to each other in complete safety need to be developed.

This study of the March on Washington Movement (MOWM) investigates the operations of the national office and examines its interactions with local branches, particularly in St. Louis. As the organization's president, A. Philip Randolph and members of the Brotherhood of Sleeping Car Porters (BSCP) such as Benjamin McLaurin and T.D. McNeal are important figures in this story. African American women such as Layle Lane, E. Pauline Myers, and Anna Arnold Hedgeman ran MOWM's national office. Of particular importance to this study is Myers' tenure as executive secretary. Working out of Harlem, she corresponded with MOWM's twenty-six local chapters, spending considerable time espousing the rationale and ideology of Non-Violent Goodwill Direct Action, a trademark protest technique developed and implemented alongside Fellowship of Reconciliation members Bayard Rustin and James Farmer. As a nationally recognized African American protest organization fighting for a "Double V" against fascism and racism during the Second World War, MOWM accrued political capital by the agitation of its local affiliates. In some cases, like in Washington, D.C., volunteers lacked the ability to forge effective protests. In St. Louis, however, BSCP official T.D. McNeal led a MOWM branch that was among the nation's most active. David Grant, Thelma Maddox, Nita Blackwell, and Leyton Weston are some of the thousands joining McNeal over a three-year period to picket U.S. Cartridge and Carter Carburetor for violating the anti-discrimination clause in Executive Order 8802, lobby Southwestern Bell Telephone to expand employment opportunities for African Americans, stage a summer of sit-ins at lunch counters in the city's largest department stores, and lead a general push for a "Double V" against fascism and racism. This study of MOWM demonstrates that the structural dynamics of protest groups often include a discrepancy between policies laid out by the organization's national office and the activity of its local branches. While national officials from MOWM and National Organization for the Advancement of Colored People had an ambivalent relationship with each other, inter-organizational tension was locally muted as grassroots activists aligned themselves with whichever group appeared most effective. During the Second World War, this was often MOWM.

Several philosophical problems are based on an analogy between a real situation and a probabilistic model. Such problems are based on urn analogies. The present dissertation aims to describe and implement a methodology oriented towards the resolution of philosophical problems based on an urn analogy. This methodology is based on the use of the n-universes. To this end, I describe first the n-universes in a detailed way. I also discuss the difficulties of the theory of n-universes related to the demultiplication of the criteria and to the relation one/many between the objects and a given criterion.On the one hand, I present an application of the framework of n-universes to the Doomsday argument and to the problems recently appeared in the literature in keeping with the Doomsday argument. My concern is also with showing how the application of the framework of n-universes to several problems and thought experiments related to the Doomsday argument helps clarifying the problem data and making disappear the associated ambiguity. I present then an analysis of the following problems related to the Doomsday argument: the two urn case, God's Coin Toss, the Sleeping Beauty Problem, the Presumptuous Philosopher, Lazy Adam, and the Shooting-Room Paradox. I present lastly a solution to the Doomsday argument, based on a third route, by contrast to two types of solutions classically described.On the other hand, I present an application of the framework of n-universes to Goodman's paradox. I replace first Goodman's statement in the framework of n-universes. I propose then a solution to the paradox, based on a distinction between two different modelizations of Goodman's statement in two structurally different n-universes.

Indiana University-Purdue University Indianapolis (IUPUI) / This thesis focuses on the history of human impact on North Manitou Island, Michigan, the management of natural and cultural resources on the island by Sleeping Bear Dunes National Lakeshore, and the often conflicting beliefs and attitudes about wilderness and cultural resources that influenced (and continue to influence) management of the island by Sleeping Bear’s administrators.

Mental suffering costs the society amounts of money every year due to sick leave, suicide and general production loss. Posttraumatic stress disorder (PTSD) is one of the conditions that can affect anyone who has experienced a traumatic event.  This thesis examines the positive experiences and limitations of the treatment form Imagery rehearsal therapy for war veterans’ post-traumatic nightmares. The results show that this treatment reduces post-trauma nightmares both in frequency and in intensity. It also improves the quality of sleep and reduces PTSD- and depression symptoms. However, veterans with multiple traumas as a basis for their PTSD may need more sessions of imagery rehearsal therapy. The results also indicate that the treatment also can show positive results in the reduction of the nightmare frequency and intensity in individuals who suffer from other types of traumatic nightmares than the war veterans had. / Psykiskt lidande kostar samhället stora summor årligen genom sjukskrivningar, självmord och allmänt produktionsbortfall. Posttraumatiskt stressyndrom (PTSD) är ett av dessa sjukdomstillstånd och kan drabba vem som helst som upplevt en traumatisk händelse. Detta examensarbete undersöker de positiva erfarenheterna av och begränsningarna hos behandlingsformen imagery rehearsal therapy vid posttraumatiska mardrömmar hos krigsveteraner. Resultatet visar att denna behandlingsform minskar posttraumatiska mardrömmar både i frekvens och i intensitet. Den förbättrar även sömnkvalitén, samt minskar PTSD-och depressionssymptom. Dock, så kankrigsveteraner med flera trauman som grund för deras PTSD behöva fler sessioner av imagery rehearsal therapy. Resultaten visar också att behandlingen även kan visa positiva resultat i minskning av mardrömmars frekvens och intensitet hos individer som lider av andra typer av traumatiska mardrömmar än de krigsveteranerna hade.

Die Gentherapie hämatopoetischer Stammzellen (HSCs) besitzt das Potenzial, verschiedene erbliche, nur symptomatisch behandelbare, Erkrankungen dauerhaft zu heilen. Die Mehrheit der aktuell angewandten Verfahren dazu, basiert auf der Isolation von hämatopoetischen Stammzellen, der ex vivo Modifikation dieser Zellen durch retrovirale Vektoren und der Reinfusion der modifizierten Zellen in den immunsupprimierten Patienten. Dieser Ansatz ist mit einer Reihe von Nachteilen verbunden, unter anderem einem teilweisen Verlust des Rekonstitutionsvermögens der Stammzellen nach ex vivo Kultur oder der Gefahr der Transformation durch Integration des retroviralen Vektorgenoms. Darüber hinaus sind aktuelle Gentherapieansätze mit hohen Kosten und großem logistischem Aufwand verbunden, was den Zugang zu diesen Behandlungen für potentielle Patienten stark einschränkt. Die vorliegende Arbeit verfolgt einen neuen Ansatz zur Gentherapie von HSCs, der auf der Mobilisierung von Stammzellen aus dem Knochenmark in den peripheren Blutstrom und der Transduktion dieser Stammzellen mit adenoviralen Vektoren basiert. Hierbei codieren die Vektoren sowohl ein Transgen als auch eine Integrationsmaschinerie. Der erste Teil der Arbeit belegt in einem humanen CD46-transgenen Mausmodell, dass adenovirale Vektoren der ersten Generation in der Lage sind, mobilisierte HSCs im Blut zu transduzieren und dass es den so transduzierten Stammzellen möglich ist, zurück ins Knochenmark zu migrieren und dort das Transgen zu exprimieren. Allerdings wurde im Verlauf von zwei Wochen ein Rückgang der Transgenexpression beobachtet. Um dies zu umgehen, wurde ein adenovirales Vektorsystem der dritten Generation genutzt, das eine hochaktive Sleeping Beauty Transposase, zum Zweck der Transgenintegration, codiert. Dieses System ermöglichte die stabile Genmodifikation mobilisierter hämatopoetischer Stammzellen nach intravenöser Injektion. Die Expression des Transgens konnte über längere Zeitspannen (bis 12 Wochen) beobachtet werden. Die modifizeirten Stammzellen waren darüber hinaus in der Lage, genmodifizierte Kolonien in vitro zu bilden und das hämatopoetische System letal bestrahlter Mäuse nach Knochenmarkstransplantation zu rekonstituieren. Es wurde somit gezeigt, dass HSCs nach in vivo Modifikation weiterhin funktional waren. / The gene therapy of hematopoietic stem cells holds the potential for curative treatment of several otherwise incurable inherited diseases. The majority of current gene therapy treatments relies on the collection of hematopoietic stem cells, their ex vivo modification with retroviral vectors and their transplantation into a myeloconditioned patient. This approach entails several disadvantages, including a reduction of stem cell engraftment potential after ex vivo culture and the potential danger of integrational mutagenesis. In addition, the high costs and complex logistics of this approach limit the access of patients to gene therapeutic regimens. This work explores an alternative approach to hematopoietic stem cell (HSC) gene therapy, termed stem cell in vivo transduction. This approach is based on the mobilization of HSCs from the bone marrow into the peripheral blood and the transduction of the stem cells with adenoviral vectors delivering a transgene as well as a transgene integration machinery. In the first part of this work, it was shown that first-generation adenoviral vectors could be used for the transduction of mobilized HSCs in the periphery of human CD46-transgenic mice. Further, the transduced HSCs were able to home back to the bone marrow and express the transgene. However, over the course of 14 days, a loss of transgene expression in HSCs was observed. To ameliorate these shortcomings, helper-dependent adenoviral vectors encoding a hyperactive Sleeping Beauty transposase for transgene integration were used for stable gene modification of hematopoietic stem cells following intravenous vector administration in mobilized human CD46-transgenic mice. Using this improved vector platform, gene marking of bone marrow HSCs could be observed for extended periods of time (up to 12 weeks). Further, the functionality of the modified HSCs was demonstrated both in colony-forming progenitor assays as well as through the transplantation of gene-modified HSCs into lethally irradiated recipients. Transplantation of modified HSCsled to long-term multi-lineage reconstitution showing that gene-modified stem cells were fully functional. Subsequently the safety of systemic vector administration in mobilized hosts as well as of the Sleeping Beauty-mediated transgene integration was assessed in human CD46- transgenic mice. Lastly, the stem cell in vivo transduction approach was employed in NOG mice transplanted with human CD34+ cells, as well as in Macaca nemestrina non-human primates.