Células de mamíferos são os hospedeiros mais frequentemente utilizados para a fabricação de proteínas biofarmacêuticas e para a produção de vacinas virais, A qualidade é um elemento-chave para o estabelecimento de um processo de bioconversão eficiente. No presente trabalho utilizamos a linhagem de células BHK- 21C13(Baby Hamster Kidney) adaptadas para cultivo em suspensão. O uso de Soro Fetal Bovino (SFB) é tradicionalmente utilizado, sendo considerado um suplemento universal, pois permite o crescimento em várias linhagens de células de mamíferos; porém, uso de SFB apresenta risco de infecção por prions, variabilidade entre lotes e aumento no custo em etapa de purificação (Downstream processing). O objetivo do presente trabalho foi comparar o cultivo de células BHK-21 C13 entre dois meios suplementados com SFB e sem SFB, através do estudo cinético para cultivo em suspensão estático e agitado com frascoT, frasco spinner e biorreator, respectivamente. Os parâmetros; Xmáx e µmáx, não foram significativamente influenciados pelo meio de cultura em cultivo estático, em cultivo com agitação em frasco spinner e também no cultivo em biorreator. O tempo de duplicação ficou próximo para todas as condições testadas. A produtividade alcançada foi: 0,032x106 cel/mL.h-1 para o meio com SFB e 0,031 X106 cel/mL.h-1 para o meio sem SFB. Ao final do processo foi possível obter uma concentração celular em torno de 4,7x106 cel/mL, tanto para o cultivo com SFB quanto para o cultivo sem SFB. Dessa forma, o uso de meio de cultivo sem SFB não alterou os principais parâmetros cinéticos, não apresentando as desvantagens do uso do SFB. / Mammalian cells are the most frequently used hosts for the production of biopharmaceutical proteins and viral vaccines. Quality is a key element for the establishment of an efficient bioconversion process. In this work, we used the cell line Baby Hamster Kidney C13 (BHK-21 C13) adapted to suspension culture was used. Fetal Bovine Serum (FBS) is traditionally used and it is considered a universal insert due to its power to increase cell growth in this kind of animal cells. However, the utilization of FBS introduces risks of infection from prions, variability between batches and increase in cost associated to purification stages (downstream processing). This work aimed to compare the kinetic behaviors of BHK-21 C13 cells in two media supplemented with FBS and without FBS using both one static and two suspension systems, T-flask, spinner flask and bioreactor respectively. The parameters; Xmax and µmax were not significantly influenced by the culture medium in T- flask culture static, in spinner flask cultivation and were neither significantly influenced by growing in culture media stirred bioreactor. The doubling time was close to all conditions tested. At the end of the growth phase it was possible to obtain a nearby cell concentration of 4.7 x 106 cells / ml, both for cultivation with FBS as for FBS without cultivation. Thus, the use of culture medium without FBS did not affect the main kinetic parameters. Besides, it does not show the disadvantages of culture media using FBS.

No presente trabalho estudou-se uma nova rota para obter nanopartículas de prata e silício aleatoriamente dispersas em uma matriz nanoporosa de nanotubos de óxido de alumínio sobre alumínio. Além disso, estudou-se a aplicação deste novo material como ânodo em células a combustível alcalinas com etanol como combustível, usando a prata como catalisador na eletroxidação do etanol e da produção de H2. O processo proposto consiste na solidificação rápida mediante melt spinner de uma liga de alumínio-prata rica em alumínio (95.25% em peso de alumínio) para obter uma solução sólida supersaturada. Posteriormente foi feita uma anodização porosa em ácido oxálico e estudo eletroquímico em meio alcalino por meia hora. A morfologia da liga obtida foi caracterizada por Microscopia Eletrônica de Varredura, Difração de Raios-X, Microscopia Eletrônica de Transmissão equipado com Espectrometria de Raios X Dispersiva em Energia e avaliado o desempenho como ânodo mediante ensaios de voltametria cíclica Os resultados obtidos confirmam que o desenvolvimento de um novo processo para produzir nanopartículas cristalinas de prata com um tamanho que varia de 4 a 120 nm, com 95% delas entre 4 e 87 nm. A partir dos estudos eletroquímicos concluiu-se que a liga de Al-Ag produzida exibe um comportamento semelhante ao alumínio puro em NaOH 0.1 M e NaOH 0.1 M com 1 M de etanol. A reação entre o alumínio e o meio alcalino produz uma camada de hidrogênio que impede que a prata catalise a eletroxidação do etanol. Portanto, conclui-se que a liga de alumínio-prata produzida não é um material viável como ânodo em células a combustível alcalinas de etanol direto. Portanto, foi avaliado o método de produção de nanopartículas para uma liga Al-Si eutética (14.2% em peso). Esta liga com nanopartículas de silício apresentou um incremento no desempenho na produção de H2 de 17% comparado à liga Al-Si eutética sem o tratamento térmico. / In the present work a new route to obtain silver nanoparticles randomly dispersed in a porous Al2O3 nanotube matrix layer on aluminum was studied. Moreover, the use as an anode in alkaline fuel cells (AFC) with ethanol as combustible was studied, using the prepared surfaces as a catalyzer for the electrooxidation of ethanol. The developed process consists of the rapid solidification (quenching) through melt spinning of an aluminum-silver alloy (92.25 %wt. Al) to obtain a supersaturated solid solution, followed by a porous anodization in oxalic acid and electrochemical treatment in alkaline medium. The morphology of the alloy was characterized by Scanning Electron Microscopy, X-Ray Diffraction, Transmission Electron Microscopy and Energy Dispersive X. Ray Spectrometry and the performance of the ethanol electrooxidation was tested though cyclic voltammetry The obtained results confirm that this process produces crystalline silver nanoparticles with a size varying from 4 to 120 nm with 95% of the particles between 4 and 87 nm. The electrochemical study showed that the produced alloy exhibits a similar behavior to that of pure aluminum in the tested mediums. The reaction between the aluminum and the alkaline medium produces a gaseous hydrogen layer that impedes the catalytic action of silver on the ethanol oxidation. Moreover, it was concluded that the produced alloy is not a viable material for the use as anode for direct ethanol AFCs. Therefore, the nanoparticle production method was tested for an Al-Si near-eutectic alloy (14.2 %wt.). This alloy with silicon nanoparticles showed an increase in the performance of H2 production rate of 17% compared to that of the regular Al-Si near-eutectic alloy.

No presente trabalho estudou-se uma nova rota para obter nanopartículas de prata e silício aleatoriamente dispersas em uma matriz nanoporosa de nanotubos de óxido de alumínio sobre alumínio. Além disso, estudou-se a aplicação deste novo material como ânodo em células a combustível alcalinas com etanol como combustível, usando a prata como catalisador na eletroxidação do etanol e da produção de H2. O processo proposto consiste na solidificação rápida mediante melt spinner de uma liga de alumínio-prata rica em alumínio (95.25% em peso de alumínio) para obter uma solução sólida supersaturada. Posteriormente foi feita uma anodização porosa em ácido oxálico e estudo eletroquímico em meio alcalino por meia hora. A morfologia da liga obtida foi caracterizada por Microscopia Eletrônica de Varredura, Difração de Raios-X, Microscopia Eletrônica de Transmissão equipado com Espectrometria de Raios X Dispersiva em Energia e avaliado o desempenho como ânodo mediante ensaios de voltametria cíclica Os resultados obtidos confirmam que o desenvolvimento de um novo processo para produzir nanopartículas cristalinas de prata com um tamanho que varia de 4 a 120 nm, com 95% delas entre 4 e 87 nm. A partir dos estudos eletroquímicos concluiu-se que a liga de Al-Ag produzida exibe um comportamento semelhante ao alumínio puro em NaOH 0.1 M e NaOH 0.1 M com 1 M de etanol. A reação entre o alumínio e o meio alcalino produz uma camada de hidrogênio que impede que a prata catalise a eletroxidação do etanol. Portanto, conclui-se que a liga de alumínio-prata produzida não é um material viável como ânodo em células a combustível alcalinas de etanol direto. Portanto, foi avaliado o método de produção de nanopartículas para uma liga Al-Si eutética (14.2% em peso). Esta liga com nanopartículas de silício apresentou um incremento no desempenho na produção de H2 de 17% comparado à liga Al-Si eutética sem o tratamento térmico. / In the present work a new route to obtain silver nanoparticles randomly dispersed in a porous Al2O3 nanotube matrix layer on aluminum was studied. Moreover, the use as an anode in alkaline fuel cells (AFC) with ethanol as combustible was studied, using the prepared surfaces as a catalyzer for the electrooxidation of ethanol. The developed process consists of the rapid solidification (quenching) through melt spinning of an aluminum-silver alloy (92.25 %wt. Al) to obtain a supersaturated solid solution, followed by a porous anodization in oxalic acid and electrochemical treatment in alkaline medium. The morphology of the alloy was characterized by Scanning Electron Microscopy, X-Ray Diffraction, Transmission Electron Microscopy and Energy Dispersive X. Ray Spectrometry and the performance of the ethanol electrooxidation was tested though cyclic voltammetry The obtained results confirm that this process produces crystalline silver nanoparticles with a size varying from 4 to 120 nm with 95% of the particles between 4 and 87 nm. The electrochemical study showed that the produced alloy exhibits a similar behavior to that of pure aluminum in the tested mediums. The reaction between the aluminum and the alkaline medium produces a gaseous hydrogen layer that impedes the catalytic action of silver on the ethanol oxidation. Moreover, it was concluded that the produced alloy is not a viable material for the use as anode for direct ethanol AFCs. Therefore, the nanoparticle production method was tested for an Al-Si near-eutectic alloy (14.2 %wt.). This alloy with silicon nanoparticles showed an increase in the performance of H2 production rate of 17% compared to that of the regular Al-Si near-eutectic alloy.

Made available in DSpace on 2014-12-17T15:37:04Z (GMT). No. of bitstreams: 1 MarinaCT_DISSERT_pag1_ate_pag15.pdf: 10459545 bytes, checksum: bd3fb5e5db3d1c521824ec8eb3c3b82c (MD5) Previous issue date: 2011-03-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Several works characterize the presence of spinner dolphins at Fernando de Noronha Archipelago in the Dolphins Bay. Though, the dolphins abidance inside this cove has decreased and a new area has been occupied by the animals to achieve the same behaviors, that are resting, breeding and nursing. This area comprises the Inside Sea northeast border of Fernando s de Noronha Island, including the opposing Middle and Dog shore area, the San Antonio Bay and the Between Islands region. The aim was to characterize the dolphins occupation and describe their interactions with the tourism in this area. Data were collected in 2008 and 2009 through a fixed point observation. The study area was divided into seven sub-areas, recording: presence/absence of dolphins, days abidance endurance, abidance length in each area, estimated number of individuals, dolphins direction and speed of displacement, boats presence, interaction period, monitoring, boats attitude and velocity. The dolphins abidance time displayed the same pattern during both years of study, with the higher occupancy in the Between Islands region. Groups with farther than 200 individuals were more frequent both 2008 (46.2%) and 2009 (42.3%). Thus the displacement s slow speed as the preferred direction towards Rat Island also showed the same pattern in both years. The Between Islands region also presented the boats major abidance near the dolphins groups. Boats moved farther in slow speed (95%) than at high speed (5%). The legislation s compliance for the cetaceans protection occurred in 89.7% of 2.839 interactions between boats and spinners, in which this variable was recorded. Whenever boats moved at a slow speed during the meetings with spinner dolphins groups, animals also moved at a slow speed (n = 337), significantly more than the fast displacements (n = 128) ix or "porpoise" (n = 4) (X2 = 318.543, p = -0.001). When boats quickly passed by groups, a significant difference between the dolphins displacement speeds was observed (X2 = 18.264, p =- 0.001), however, the slow (47%) and fast (47% ) displacements frequency was equal, noted the difference with the porpoise displacements (6%), which had the lowest frequency. Data indicate the establishment of a new occupation pattern of the spinner dolphins at Fernando de Noronha, with the Between Islands area being of great importance to the dolphins habits and currently the main area of the boats meeting with the dolphins, showing the need of new conservation measures in this area / Diversos trabalhos caracterizam a presen?a de golfinhos-rotadores no Arquip?lago Fernando de Noronha na Ba?a dos Golfinhos. Entretanto, o tempo de perman?ncia dos golfinhos nesta enseada tem diminu?do e uma nova ?rea vem sendo ocupada pelos animais para a realiza??o dos mesmos comportamentos, que s?o descanso, reprodu??o e cuidados com os filhotes. Esta ?rea compreende a extremidade nordeste do Mar de Dentro da Ilha Fernando de Noronha, incluindo a ?rea defronte as praias do Meio e do Cachorro e toda a Ba?a de Santo Ant?nio e Regi?o Entre Ilhas. O presente estudo objetivou caracterizar a ocupa??o dos golfinhos e descrever as intera??es com o turismo nesta ?rea. Os dados foram coletados atrav?s de ponto fixo em 2008 e 2009. A ?rea de estudo foi dividida em sete sub?reas e os registros foram: presen?a/aus?ncia de golfinhos, tempo de perman?ncia no dia, tempo de perman?ncia em cada sub?rea, estimativa do n?mero de indiv?duos, dire??o e velocidade de deslocamento dos golfinhos, presen?a de barcos, tempo de intera??o, acompanhamento, atitude e velocidade de deslocamento das embarca??es. O tempo de perman?ncia dos golfinhos mostrou o mesmo padr?o nos dois anos do estudo, sendo a Regi?o Entre Ilhas a com maior ocupa??o. Os agrupamentos com mais de 200 indiv?duos foram os mais freq?entes, tanto em 2008 (46,2%) como em 2009 (42,3%). A dire??o preferencial de deslocamento sentido Ilha Rata e a velocidade de deslocamento lenta tamb?m mostraram o mesmo padr?o nos dois anos. A Regi?o Entre Ilhas tamb?m foi a que apresentou o maior tempo de perman?ncia dos barcos pr?ximos aos grupos de golfinhos. As embarca??es significativamente se deslocaram mais em velocidade lenta (95%) dos que em alta velocidade (5%). O cumprimento da legisla??o de prote??o aos cet?ceos ocorreu em 89,7% das 2.839 intera??es entre barcos e vii rotadores em que foi registrada esta vari?vel. Quando as embarca??es se deslocaram em velocidade lenta durante os encontros com os agrupamentos de golfinhos-rotadores, os animais tamb?m se deslocaram em velocidade lenta (n=337), significativamente mais do que os deslocamentos r?pidos (n=128) ou porpoise (n=4) (X2=318,543; p= -0,001). J? quando as embarca??es passaram r?pido pelos agrupamentos de golfinhos, observou-se diferen?a significativa entre as velocidades de deslocamentos dos golfinhos (X2=18,264; p=-0,001), entretanto, as freq??ncias de deslocamentos lentos (47%) e r?pidos (47%) foi igual, sendo a diferen?a notada com os deslocamentos em porpoise (6%), que apresentou a menor freq??ncia. Os dados indicam o estabelecimento de um novo padr?o de ocupa??o dos golfinhosrotadores em Fernando de Noronha, sendo a Regi?o Entre Ilhas de grande import?ncia para os h?bitos dos golfinhos e atualmente a principal ?rea de encontro das embarca??es com os golfinhos, mostrando a necessidade de novas medidas de conserva??o nesta ?rea

No presente trabalho estudou-se uma nova rota para obter nanopartículas de prata e silício aleatoriamente dispersas em uma matriz nanoporosa de nanotubos de óxido de alumínio sobre alumínio. Além disso, estudou-se a aplicação deste novo material como ânodo em células a combustível alcalinas com etanol como combustível, usando a prata como catalisador na eletroxidação do etanol e da produção de H2. O processo proposto consiste na solidificação rápida mediante melt spinner de uma liga de alumínio-prata rica em alumínio (95.25% em peso de alumínio) para obter uma solução sólida supersaturada. Posteriormente foi feita uma anodização porosa em ácido oxálico e estudo eletroquímico em meio alcalino por meia hora. A morfologia da liga obtida foi caracterizada por Microscopia Eletrônica de Varredura, Difração de Raios-X, Microscopia Eletrônica de Transmissão equipado com Espectrometria de Raios X Dispersiva em Energia e avaliado o desempenho como ânodo mediante ensaios de voltametria cíclica Os resultados obtidos confirmam que o desenvolvimento de um novo processo para produzir nanopartículas cristalinas de prata com um tamanho que varia de 4 a 120 nm, com 95% delas entre 4 e 87 nm. A partir dos estudos eletroquímicos concluiu-se que a liga de Al-Ag produzida exibe um comportamento semelhante ao alumínio puro em NaOH 0.1 M e NaOH 0.1 M com 1 M de etanol. A reação entre o alumínio e o meio alcalino produz uma camada de hidrogênio que impede que a prata catalise a eletroxidação do etanol. Portanto, conclui-se que a liga de alumínio-prata produzida não é um material viável como ânodo em células a combustível alcalinas de etanol direto. Portanto, foi avaliado o método de produção de nanopartículas para uma liga Al-Si eutética (14.2% em peso). Esta liga com nanopartículas de silício apresentou um incremento no desempenho na produção de H2 de 17% comparado à liga Al-Si eutética sem o tratamento térmico. / In the present work a new route to obtain silver nanoparticles randomly dispersed in a porous Al2O3 nanotube matrix layer on aluminum was studied. Moreover, the use as an anode in alkaline fuel cells (AFC) with ethanol as combustible was studied, using the prepared surfaces as a catalyzer for the electrooxidation of ethanol. The developed process consists of the rapid solidification (quenching) through melt spinning of an aluminum-silver alloy (92.25 %wt. Al) to obtain a supersaturated solid solution, followed by a porous anodization in oxalic acid and electrochemical treatment in alkaline medium. The morphology of the alloy was characterized by Scanning Electron Microscopy, X-Ray Diffraction, Transmission Electron Microscopy and Energy Dispersive X. Ray Spectrometry and the performance of the ethanol electrooxidation was tested though cyclic voltammetry The obtained results confirm that this process produces crystalline silver nanoparticles with a size varying from 4 to 120 nm with 95% of the particles between 4 and 87 nm. The electrochemical study showed that the produced alloy exhibits a similar behavior to that of pure aluminum in the tested mediums. The reaction between the aluminum and the alkaline medium produces a gaseous hydrogen layer that impedes the catalytic action of silver on the ethanol oxidation. Moreover, it was concluded that the produced alloy is not a viable material for the use as anode for direct ethanol AFCs. Therefore, the nanoparticle production method was tested for an Al-Si near-eutectic alloy (14.2 %wt.). This alloy with silicon nanoparticles showed an increase in the performance of H2 production rate of 17% compared to that of the regular Al-Si near-eutectic alloy.

Whale-watching provides economic opportunities worldwide and particularly proliferates in developing countries, such as the Philippines. The sustainability of whale-watching is increasingly debated as these activities also negatively impact cetaceans through changes in behavior, communication, habitat use, morbidity, mortality, and life-history parameters. This study evaluated the total annual cost, revenue, and profit of whale-watching operators in Bais, Philippines, and predicted the changes in the population for spinner dolphin Stenella longirostris with varying levels of whale-watching effort. Total revenue was 3,805,077 PHP ($92,478 USD) while total cost was 5,649,094 PHP ($137,294 USD) with a discount rate of ten percent. The total annual profit of whale-watching in Bais was – 1,844,017 PHP (– $44,817 USD). On average, each operator in Bais lost 160,350 PHP ($3,897 USD) per year from whale-watching. Through time, the spinner dolphin population decreased as it was exposed to more vessels, causing effort to increase, and thus decreased profit for operators. Under current whale-watching effort, the spinner dolphin population was predicted to decrease by 94 percent in 25 years. If Bais reduced effort in their operations to only three vessels whale-watching per day, the spinner dolphin population increased to 80 percent of its initial population size. This was the first study to predict the spinner dolphin population and estimate the total annual profit from whale-watching in Bais, Philippines. It provided data to locals for efficient, profitable, and sustainable decisions in whale-watching operations.

The first part of this thesis describes the current technologies of mechanical spin-ning. This is a ring, rotor and air-jet spinning. The product of spinning machines is yarn, the basic material for production textile. The next section describes in detail the current state of the node manipulator lifter tube on spinning machine TORNADO J20, by concern RIETER. It describes the structure, kinematics, duty cycle and requirements for the manipulator. The following optimization options which are described more options for solutions of individual functions of the manipulator, on the end is selected by multi-criteria analysis. The best solution that is elaborate in the next section. The author has created 3-D model optimized lifter tube. Positioning mechanism maintains the current kinematics, it is very similar. But gripper was designed as a passive, thus reduce the load positioning mechanism. Optimization was performed primarily to reduce overall production costs and to reduce weight manipulator. The conclusion is a comparison of current and new solutions. There are also eco-nomic recovery to.

The diploma thesis is focused on the revitalization of the dilapidated area of the former textile factory in Brno, Obřany. Areal is located in the city's peripheral parts between Maloměřice and Obřany. The location of the areal creates a strategic potential for creating a closer relationship between the central part of the city and its peripheries which allows urban activities spread to the distant area. The main target of the paper was to promote the relationship between the island and the water element - the river which brings a unique composition for the creation of a new central point of the urban part, which in Obřany currently lacking. The main focus of the project was to support the local potential by expanding the range of urban activities and recreational opportunities with respect for industrial heritage of Brno. It was necessary to made a radical interventions for island revitalization. Cleaning the island from inappropriate, structural, statically or architecturally inadequate buildings and re-opening the space for public was needed. The former spinning factory was therefore a key element of the entire project. It is due to its monumental qualities predestined to place the main and dominant function which after a previous analysis and survey was allocated to the textile museum. As the opposite of this huge structure there were designed a free and grassy area of the park which has been cleaned from the old unsatisfactory objects. The preserved buildings of the former textile factories were individually revitalized and designed with new suitable functions.

Bioreactors are devices used for the growth of tissues in a laboratory environment. They exist in many different forms, each designed to enable the production of high-quality tissues. The dynamic environment within bioreactors is known to significantly affect the growth and development of the tissue. Chondrocytes, the building blocks of articular cartilage, for example, are stimulated by mechanical stresses such as shear, as compared with those in tissues grown under static incubation conditions. On the other hand, high shear can damage cells. Consequently the shear-stress level has to be controlled in order to optimize the design and the operating conditions of bioreactors. Spinner flasks have been used for the production of articular cartilage in vitro. Assuming the existence of a relation between the cellular glycosaminoglycan (GAG) synthesis and the local shear stresses on the construct surfaces, this research focuses on the development of a model for cartilage growth in such devices. The flow produced in a model spinner flask is characterized experimentally using particle-image velocimetry (PIV). A computational fluid dynamic (CFD) model validated with respect to the laboratory measurements is constructed in order to predict the local shear stresses on the construct surfaces. Tissue growth experiments conducted in the prototype bioreactor permit construct histologies and GAG contents to be analyzed and then correlated with the shear-stress predictions. The integration of this relation into the CFD model enables the prediction of GAG synthesis through convective effects. Coupling this convective model to an existing diffusive model produces a complete cartilage-growth model for use in aiding the optimization of existing bioreactors, and in the design of new ones.

Made available in DSpace on 2014-12-17T15:36:53Z (GMT). No. of bitstreams: 1 LiisaH.pdf: 524666 bytes, checksum: 8ed111d1e268e386d58d4e05b903d400 (MD5) Previous issue date: 2009-09-12 / Studies indicate that spinner dolphins use the Ba?a dos Golfinhos, in Fernando de Noronha Archipelago, for resting, reproduction, parental care and protection against shark attacks. The present study had the purpose of verifying the seasonality of spinner dolphin newborns and calves in relation to the months of the year and the pluviometric seasons (dry and rainy), as well as their interaction with the number and gender of accompanying adults and their positioning in relation to the adults (vertical, horizontal, and depth) in the above mentioned bay. The analysis were made out of photo records of dolphins collected between 2000 and 2006 (seasonality) and between 1995 and 2006 (interaction) both using ad libitum sampling method during free dives. To determine the age category, the reason between the smaller dolphin s total body length and the bigger dolphin s total body length was calculated. The dolphins were then divided into three age groups: adults, newborns and calves. Those with total body length ≥ 170cm were considered adults, newborns up to 105cm, and calves from 106cm to 128cm. In addition, the secondary characters described in literature were used to identify newborns and calves. The adults had dimensions of total lenght ≥170cm , the newborns until 105cm and calves between 106cm and 128cm. I addition, secondary characteristics described in the literature were used to indentify newborns and calves. The number of spinner dolphin newborns was greater in the month of April and higher during the rainy season. Throughout the months and pluviometric seasons (pluviometer/pluvial metric), the number of calves did not have a significant difference. Concerning to the presence of newborns and calves age groups at Ba?a dos Golfinhos, there was not a significant difference. It was possible to identify the gender of the escorting adults as (42), 95.24% being females and 4.76% males. Newborns were more frequently seen in the company of two adults, whereas calves were more often accompanied by more than two adults However, there was not a significant difference for the newborns, whereas for the calves there was a significant difference for those classified as loners and those accompanied by more than two adults. When in vertical positioning, the newborns and calves were more frequently observed in inferior position with some difference demonstrated between some of that. While in horizontal positioning both age groups were more often seen in posterior position, also with differences between them. In the depth perspective newborns and calves were positioned anterior, however with significant difference for the calves. The occurrence of a peak of newborns in the months of April may indicate the existence of a birth seasonality pattern for the beginning of the rainy season, with births scattered throughout the year. The results for the positions and escorting of newborns and calves are related to protection and suckling. These conditions reinforce the importance of the area when it comes to the care for offspring, which calls for the creation of conservation rules to the area, especially during those months with greater occurrence of newborns / Estudos indicam que golfinhos-rotadores utilizam a Ba?a dos Golfinhos de Fernando de Noronha para descanso, cuidado parental, acasalamentos e prote??o contra tubar?es. O presente estudo objetivou verificar a sazonalidade de ocorr?ncia de rec?m-nascidos e filhotes de golfinhos-rotadores durante os meses do ano e esta??es pluviom?tricas (seca e chuvosa), e a intera??o destes quanto ? quantidade e sexo de adultos acompanhantes, posicionamento dos rec?m-nascidos e filhotes em rela??o aos adultos (entre as dimen??es vertical, horizontal e profundidade) na referida enseada. As an?lises foram desenvolvidas a partir de registros fotogr?ficos dos golfinhos coletados entre 2000 e 2006 (sazonalidade) e entre 1995 e 2006 (intera??o), ambos atrav?s de amostragem ad libitum em mergulhos livres. Para a determina??o das faixas et?rias, calculou-se a raz?o entre o comprimento total do golfinho menor pelo maior mais pr?ximo a ele na mesma fotografia. Os golfinhos foram divididos em tr?s classes et?rias: adultos, rec?m-nascidos e filhotes. Indiv?duos adultos apresentavam propor??es de comprimento total ≥170cm, rec?m-nascidos at? 105cm e filhotes entre 106cm e 128cm. Em adi??o, foram utilizados caracteres secund?rios descritos na literatura para identificar rec?m-nascidos e filhotes. O maior n?mero de rec?m-nascidos foi registrado no m?s de abril e maior na esta??o chuvosa. Em rela??o ao n?mero de filhotes, n?o houve diferen?a significativa ao longo dos meses e nas esta??es pluviom?tricas. N?o houve diferen?a significatica com rela??o faixas et?rias de rec?m-nascidos e filhotes presentes na Ba?a dos Golfinhos. Entre os adultos acompanhando rec?m-nascidos e filhotes, no caso em que foi poss?vel identificar o sexo dos indiv?duos (42), 95,24% eram f?meas e 4,76% machos. Os rec?m-nascidos estavam em companhia mais freq?ente de 2 adultos, e os filhotes com mais de 2 adultos. Por?m, n?o foi registrada diferen?a para os rec?m-nascidos, ao contr?rio dos filhotes, que diferiram significativamente entre estar sozinho e os demais acompanhamentos. Com rela??o aos posisionamentos nas tr?s dimen??es analisadas, para o posicionamento vertical os rec?m-nascidos e filhotes estavam mais na posi??o inferior, demonstrando diferen?as entre algumas delas. Para a posi??o horizontal, os rec?m-nascidos e filhotes encontravam-se mais posterior, tamb?m com diferen?as entre elas. Com rela??o ? perspectiva profundidade, rec?m-nascidos e filhotes estavam posicionados na frente, por?m apresentou-se diferen?a significativa somente para os filhotes entre as demais. A presen?a do pico de ocorr?ncia de rec?m-nascidos no m?s de abril pode indicar a exist?ncia de um padr?o sazonal de nascimento no in?cio da esta??o das chuvas, por?m tamb?m com a exist?ncia de nascimentos difusos no decorrer do ano. As posi??es e acompanhamentos dos rec?m-nascidos e filhotes se relacionam ? prote??o e amamenta??o. Essas condi??es refor?am a import?ncia da ?rea para o cuidado da prole, necessitando de regras de acesso ? ?rea, principalmente durante os meses com maior ocorr?ncia de rec?m-nascidos