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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
41

Development of a layout for effective use of space in a block stacking warehouse

Gunnervald, Sebastian, Gustafsson, Viktor January 2017 (has links)
Historically, the paper market has consisted of few customers with high demands. However, the market has changed and nowadays it consists of many customer with lower demands, which causes problems in the warehouse process of Braviken Papermill. The main problem is that the utilization rates in the storage bays are low due to the relatively large bays compared to the order quantities. The purpose of this project is to develop a new set of bays that fits the current market and increases the utilization rates. The goal of the new set is to decrease the amount of manual operations and instead use the full potential of the allocation system.    The new set of bays consist of four bay types, dimensioned for 2, 4, 8 and 16 reel stacks respectively. Several experiments are done, and the new set of bays performs better than the current one, in terms of the utilization rates for storage events in the bays. The average utilization rate is significantly higher for the new set compared to the current. A decrease in effective storage space can be compensated by an increased degree of utilization. Also, it is beneficial with more and smaller bays in a market situation like the one of Holmen Paper.
42

Entwicklung von Full-Waveform Stackingverfahren zur Detektion schwacher Gewässerbodenechos in der Laserbathymetrie

Mader, David 20 June 2023 (has links)
Airborne Laserbathymetrie stellt eine effiziente und flächenhafte Messmethode für die Erfassung der sich ständig im Wandel befindlichen Gewässersohlen von Inlandgewässern und küstennahen Flachwasserbereichen dar. Bei diesem Verfahren wird ein kurzer grüner Laserpuls ausgesandt, welcher mit allen Objekten entlang des Laserpulspfades interagiert (z.B. Wasseroberfläche und Gewässerboden). Die zum Sensor zurückgestreuten Laserpulsanteile (Echos) werden in einem zeitlich hochaufgelösten Messsignal (Full-Waveform) digitalisiert und gespeichert. Allerdings ist das Messverfahren aufgrund von Gewässertrübung in seiner Eindringtiefe in den Wasserkörper limitiert. Die Gewässerbodenechos werden bei zunehmender Gewässertiefe schwächer, bis sie nicht mehr zuverlässig detektierbar sind. Diese Arbeit zeigt, wie mit neuartigen Methoden schwache Gewässerbodenechos in Full-Waveforms detektiert werden können, welche durch die Standardauswerteverfahren nicht mehr berücksichtigt werden. Im Kernstück der Arbeit werden zwei Verfahren vorgestellt, die auf einer gemeinsamen Auswertung dicht benachbarter Messdaten basieren. Unter der Annahme eines stetigen Gewässerbodens mit geringer bis moderater Geländeneigung führt die Zusammenfassung mehrerer Full-Waveforms zu einer Verbesserung des Signal/Rausch-Verhältnisses und einer Verstärkung von schwachen Gewässerbodenechos, welche folglich zuverlässiger detektiert werden können. Die Ergebnisse zeigen eine erhebliche Erhöhung der auswertbaren Gewässertiefe (bis zu +30 %), wodurch eine deutlich größere Fläche des Gewässerbodens abgedeckt werden konnte (Flächenzuwachs von bis zu +113 %). In umfassenden Analysen der Ergebnisse konnte nachgewiesen werden, dass die hinzugewonnenen Gewässerbodenpunkte eine gute Repräsentation des Gewässerbodens darstellen. Somit leisten die in dieser Arbeit entwickelten Verfahren einen wertvollen Beitrag zur Steigerung der eingangs beschriebenen Effizienz der Airborne Laserbathymetrie.:Kurzfassung Abstract 1 Einleitung 1.1 Motivation 1.2 Ziele der Dissertation 1.3 Aufbau der Arbeit 2 Einführung in bathymetrische Messverfahren 2.1 Hydrographie und Bathymetrie 2.2 Airborne LiDAR Bathymetrie 2.2.1 Grundlagen Airborne Laserscanning 2.2.2 Der Pfad des Laserpulses 2.2.3 Fehlereinflüsse 2.3 Die Full-Waveform 2.3.1 Aufbau und Merkmale einer Full-Waveform 2.3.2 Systemwaveform 2.3.3 Full-Waveform Auswerteverfahren 2.4 Hydroakustische Messverfahren 2.4.1 Messprinzip 2.4.2 Echolot Varianten 2.4.3 Fehlereinflüsse 3 Nichtlineare Full-Waveform Stacking-Verfahren zur Detektion und Extraktion von Gewässerbodenpunkten – Beitrag 1, Beitrag 2, Beitrag 3 3.1 Signalbasiertes nichtlineares Full-Waveform Stacking 3.2 Volumetrisches nichtlineares Ortho-Full-Waveform Stacking 4 Anwendung von nichtlinearen Full-Waveform Stacking-Methoden auf maritime Gewässer – Beitrag 4 4.1 Studiengebiet in der Nordsee 4.2 Datengrundlage 4.3 Erste Ergebnisse einer Pilotstudie in küstennahen Bereichen der Nordsee 4.4 Untersuchungsgebiet 4.5 Klassifikation der Wasseroberflächenpunkte 4.6 Visualisierung der Ergebnisse 4.7 Genauigkeit und Zuverlässigkeit 4.8 Mehrwert der Verfahren 5 Potential der Full-Waveform Stacking-Methoden zur Ableitung der Gewässertrübung – Beitrag 5 6 Diskussion und weiterführende Arbeiten 6.1 Geometrische Modellierung der Laserpulsausbreitung 6.2 Einfluss der Gewässereigenschaften auf die Gewässerbodenbestimmung 6.3 Unterschätzung der Wasseroberfläche 6.4 Nutzung von Gewässertrübungsinformation für die Beurteilung der Zuverlässigkeit der Gewässertiefenbestimmung 6.5 Auswirkung der Nachbarschaftsdefinition beim signalbasiertem Full-Waveform Stacking 6.6 Gegenüberstellung signalbasiertes und volumetrisches Full-Waveform Stacking 6.7 Erweiterung des Full-Waveform Stackings mit dem Multi-Layer-Ansatz 7 Fazit der Dissertation 7.1 Zusammenfassung 7.2 Einordnung der Dissertation 7.3 Mehrwert der Dissertation Literaturverzeichnis Abbildungsverzeichnis Tabellenverzeichnis Symbolverzeichnis Abkürzungsverzeichnis / Airborne laser bathymetry is an efficient and area-wide measurement method for the detection of the permanently changing water bottoms of inland waters and shallow water areas close to the coast. In this method, a short green laser pulse is emitted, which interacts with all objects along the laser pulse path (e.g. water surface and bottom). The backscattered laser pulse components (echoes) are digitized and stored in a high temporal resolution measurement signal (full-waveform). However, the measurement method is limited in its penetration depth into the water body due to water turbidity. The water bottom echoes become weaker as the water depth increases until they are no longer reliably detectable. This work shows how novel methods can be used to detect weak water bottom echoes in full-waveforms that are no longer accounted for by standard processing methods. In the core of the work, two methods are presented which are based on a joint evaluation of closely adjacent measurement data. Under the assumption of a steady water bottom with low to moderate slope, the combination of several full-waveforms leads to an improvement of the signal-to-noise ratio and an enhancement of weak water bottom echoes, which consequently can be detected more reliably. The results show a significant increase in the analyzable water depth (up to +30 %), allowing a much larger area of the water bottom to be covered (increase up to +113 %). Comprehensive analyses of the results proved that the added water bottom points are a good representation of the water bottom. Thus, the methods developed in this work constitute a valuable contribution to increase the efficiency of airborne laser bathymetry described at the beginning.:Kurzfassung Abstract 1 Einleitung 1.1 Motivation 1.2 Ziele der Dissertation 1.3 Aufbau der Arbeit 2 Einführung in bathymetrische Messverfahren 2.1 Hydrographie und Bathymetrie 2.2 Airborne LiDAR Bathymetrie 2.2.1 Grundlagen Airborne Laserscanning 2.2.2 Der Pfad des Laserpulses 2.2.3 Fehlereinflüsse 2.3 Die Full-Waveform 2.3.1 Aufbau und Merkmale einer Full-Waveform 2.3.2 Systemwaveform 2.3.3 Full-Waveform Auswerteverfahren 2.4 Hydroakustische Messverfahren 2.4.1 Messprinzip 2.4.2 Echolot Varianten 2.4.3 Fehlereinflüsse 3 Nichtlineare Full-Waveform Stacking-Verfahren zur Detektion und Extraktion von Gewässerbodenpunkten – Beitrag 1, Beitrag 2, Beitrag 3 3.1 Signalbasiertes nichtlineares Full-Waveform Stacking 3.2 Volumetrisches nichtlineares Ortho-Full-Waveform Stacking 4 Anwendung von nichtlinearen Full-Waveform Stacking-Methoden auf maritime Gewässer – Beitrag 4 4.1 Studiengebiet in der Nordsee 4.2 Datengrundlage 4.3 Erste Ergebnisse einer Pilotstudie in küstennahen Bereichen der Nordsee 4.4 Untersuchungsgebiet 4.5 Klassifikation der Wasseroberflächenpunkte 4.6 Visualisierung der Ergebnisse 4.7 Genauigkeit und Zuverlässigkeit 4.8 Mehrwert der Verfahren 5 Potential der Full-Waveform Stacking-Methoden zur Ableitung der Gewässertrübung – Beitrag 5 6 Diskussion und weiterführende Arbeiten 6.1 Geometrische Modellierung der Laserpulsausbreitung 6.2 Einfluss der Gewässereigenschaften auf die Gewässerbodenbestimmung 6.3 Unterschätzung der Wasseroberfläche 6.4 Nutzung von Gewässertrübungsinformation für die Beurteilung der Zuverlässigkeit der Gewässertiefenbestimmung 6.5 Auswirkung der Nachbarschaftsdefinition beim signalbasiertem Full-Waveform Stacking 6.6 Gegenüberstellung signalbasiertes und volumetrisches Full-Waveform Stacking 6.7 Erweiterung des Full-Waveform Stackings mit dem Multi-Layer-Ansatz 7 Fazit der Dissertation 7.1 Zusammenfassung 7.2 Einordnung der Dissertation 7.3 Mehrwert der Dissertation Literaturverzeichnis Abbildungsverzeichnis Tabellenverzeichnis Symbolverzeichnis Abkürzungsverzeichnis
43

Effektivisering av färdigvarulager med fokus på dess aktiviteter och utformning : En fallstudie på Hallsta pappersbruk / Improved efficiency of a finished goods warehouse focusing on its activities and design : A case study at Hallsta Paper Mill

Öberg, Agnes, Östlund, Fredrik January 2022 (has links)
In recent years, Hallsta Paper Mill has experienced significant changes in the demand for paper. The ordershave become smaller and more customer-specific, while the graphical paper industry has steadily declined.As a result of these changes, the employees at Hallsta Paper Mill feel that the finished goods warehouse isno longer optimally designed, and they have identified that waste occurs in the warehouse. Therefore, thepurpose of the study was to provide suggestions on how the activities in the warehouse could become moretime-efficient and investigate how the finished goods warehouse could be designed to suit today’s demandbetter. To facilitate the answering of the purpose, three research questions were formulated: What areas ofimprovement exist in the finished goods warehouse regarding its activities and layout? What effects can beobtained by addressing the identified areas of improvement? and What solutions are recommended for thefinished goods warehouse at Hallsta Paper Mill? The study’s first research question was answered by conducting interviews, a spaghetti diagram, and ananalysis of produced paper rolls. The interviews were conducted with employees who either workoperationally or administratively in the warehouse to get a comprehensive picture of how the work isperformed and where waste occurs within the activities. The spaghetti diagram identified the movementpattern for the trucks in the warehouse and provided indications if unnecessary long transports occurred ornot. The analysis of produced paper rolls examined how well the storage compartments in the finishedgoods warehouse were dimensioned based on today’s demand. Based on the collected data, nine areas ofimprovement were identified, seven areas linked to the activities in the warehouse and two areas linked tothe layout of the warehouse. After the areas of improvement were identified, the study’s second research question was answered bydeveloping solutions within each area of improvement. To develop the solutions linked to the activities inthe warehouse, root cause analyses were performed, where each area was examined to find the root causeof the problem. For the solutions linked to the design, inspiration was instead taken from the warehouselayout and article placement literature. A total of 23 solutions were developed, 19 linked to the activitiesand four linked to the layout. For each solution, it was then analysed what effects an implementation of thesolution would provide, focusing on possible time savings. The effects were analysed with help from theliterature and the authors’ knowledge of logistics. After the effects had been analysed, each solution was assessed based on the effects identified in theprevious research question. The solutions were placed in effort-impact diagrams based on how much effortis required to implement the solution and how much impact is expected from the solution. When assessingthe impact, extra consideration was given to possible time savings, as it was the highest priority accordingto the directives from Hallsta Paper Mill. The assessment then resulted in a recommendation on how thesolutions should be approached. The less effort required and the more impact a solution would bring, thehigher priority was given to the solution. To summarise, two solutions were recommended to beimplemented immediately, nine solutions should be implemented in a second step, seven solutions shouldbe considered and investigated further, and two solutions should be rejected. The solutions aimed to makethe warehouse activities more time-efficient and suggest how the warehouse could be designed to suittoday’s demand better.
44

Bioenergy from Swedish forests : A Study of extraction methods, quality and effects for forest owners

Nilsson, Daniel January 2020 (has links)
The forest constitutes a very important element of renewable natural resources and makes a significant contribution to the Swedish bioeconomy. Biofuels are Sweden’s largest source of energy; of all the energy we use, 32% comes from biofuels, and of this approximately 85% comes from the forest and the forestry sector. In spite of this, logging residues constitute only a small component, compared to for example byproducts from sawmills and pulpindustry, and there is considered to be great potential for increasing their use. In 2019 the Swedish Forestry Agency issued new recommendations for logging residue harvest and ash recycling. This was a further development of the 2008 recommendations, which formed the foundation for how forest fuel producers work today, and were based on several decades of research into, for example, the impact on forest productivity and technological development of machinery. This practice of logging residue harvest aims to yield a dry and defoliated fuel where the needles are left at the clear felled area. However, if we are to increase the use of green renewable energy from forestry, it is very important to understand how different procurement systems affect the handling and storability of fuels from a quality perspective. It is also of great importance to understand, from the forest owners’ perspective, how removal of additional products from forestry influences nutritional balance and long-term productivity. If harvesting of logging residues does not affect long-term productivity, it is up to small-scale private forest owners to decide if removal of logging residues will be performed on their land. This thesis addresses some of these issues regarding removal of logging residues from the point of tree harvest up to the point of delivery to the energy conversion industry when the fuel chips are measured. Regarding different methods of handling of logging residues, the traditional method – dry-stacking – was compared with the, fresh-stacking method. The logging residues investigated came from stands that mainly consisted of Norway spruce (Picea abies (L.) Karst). The loads investigated in Paper 3 also came from logging of spruce-dominated forests. Both methods aim to dry the logging residues to an acceptable moisture content for delivery to the energyconversion industry. For the later part in the supply chain, moisture content measurements of logging residues were compared during a winter and summer season. The results of the studies indicate that the two methods do not create results that differ from what is allowed by the Swedish Forestry Agency and that they are quite similar with respect to dry mass- and nutrient removal from the clearfelled area. The results also show that similar yields and distributions of material are obtained from the logging residues with different stacking methods; in addition, the final felling itself, combined with the work performed by the forwarder operator, has a greater impact on the result than the method chosen for residue stacking of the logging residues. For the individual clear-felled area and the individual forest owner the increased removal associated with freshstacked logging residue has no major impact, however from a national perspective this small increase in removed logging residues may yield a supplement of between 0.5 – 1 TWh of green energy annually. Regardless of treatment, the studies indicated that the delivered fuel chips will have similar characteristics. The moisture content measurement techniques currently in use are sufficiently accurate and reliable. However, if the forest owner is unlucky and an error in measurement occurs or comminution and delivery happens during an especially wet period they may suffer a significant financial loss; indeed, it is generally not under the individual forest owners’ control when the logging residues are comminuted and delivered.
45

SAMs (self-assembled monolayers) passivation of cobalt microbumps for 3D stacking of Si chips

Hou, Lin, Derakhshandeh, Jaber, Armini, Silvia, Gerets, Carine, De Preter, Inge, June Rebibis, Kenneth, Miller, Andy, De wolf, Ingrid, Beyne, Eric 22 July 2016 (has links) (PDF)
In this paper SAM (self-assembled monolayers) is used to passivate cobalt microbumps for 3D-stacking of Si chips. The SAM deposition process is optimized, using input from characterization techniques such as water contact angle measurement, ATR, AFM and XPS analysis in order to form a monolayer of Thiols-SAM on cobalt microbumps. A 3D stacked Si chips test vehicle was used to demonstrate the effectiveness of the SAM coating on cobalt bumps by measuring the electrical continuity of daisy chains.
46

Behavior of Copper Contamination for Ultra-Thinning of 300 mm Silicon Wafer down to <5 μm

Mizushima, Yoriko, Kim, Youngsuk, Nakamura, Tomoji, Sugie, Ryuichi, Ohba, Takayuki 22 July 2016 (has links) (PDF)
Bumpless interconnects and ultra-thinning of 300 mm wafers for three-dimensional (3D) stacking technology has been studied [1, 2]. In our previous studies, wafer thinning effect using device wafers less than 10 μm was investigated [3, 4]. There was no change for the retention time before and after thinning even at 4 μm in thickness of DRAM wafer [5]. In this study, the behavior of Cu contamination on an ultra-thin Si stacked structure was investigated. Thinned Si wafers were intentionally contaminated with Cu on the backside and 250 °C of heating was carried out during the adhesive bonding and de-bonding processing. An approximately 200 nm thick damaged layer was formed at the backside of the Si wafer after thinning process and Cu particle precipitates ranged at 20 nm were observed by cross-sectional transmission electron microscopy (X-TEM). With secondary ion mass spectrometry (SIMS) and EDX analyses, Cu diffusion was not detected in the Si substrate, suggesting that the damaged layer prevents Cu diffusion from the backside.
47

UNHINDERED TRIANGULENE SALT PAIRS: SUBSTITUTION-DEPENDENT CONTACT ION PAIRING AND COMPLEX SOLVENT-SEPARATED DISCOTIC IONS IN SOLUTION

Modekrutti, Subrahmanyam 01 January 2015 (has links)
This work sought to enforce aromatic interactions between compatible π-molecular orbital systems with ionic bonding. In this case the interacting partners are oppositely charged discotic triangulene derivatives. The observed properties of the heterodimeric ion-pairs likely arise due to a hypothetical synergy between electrostatics and π-interactions. The work presented here describes investigation of putative covalency arising from this hypothetical synergy in the electrostatics driven π-stacking. In order to probe this, various hypotheses were made and experiments were designed to test their validity. The results from the experiments show existence of contact ion-pairs and complex solvent-separated discotic ions in solution. The formation of complex ion-pairs arise due to the fact that the electrostatic interaction that brings the discotic ions together is strong, but does not neutralize when the contact is made. So, the dipole created by the monopoles in a dimeric contact ion-pair can attract ions at both termini forming oligomers. This process apparently continues towards highly aggregated states and then to nanometric species and at some point the material precipitates. The propensity to aggregate and form complex-ions limited our approach to the measurement of the energetics of the ion-pairing for two reasons: (1) the observables had a complex dependence on temperature, solvent, concentration and ionic strength; and (2) the mass in solution was undergoing kinetic evolution towards solid states. The turbidimetric effects arising due to aggregate formation further complicated the extraction of weak interactions between the ions and hence effects determination of ion-pairing constants.
48

Platinum Complexes and Zinc Finger Proteins: From Target Recognition to Fixation

Tsotsoros, Samantha 01 January 2014 (has links)
Bioinorganic chemistry strives to understand the roles of metals in biological systems, whether in the form of naturally occurring or addition of non-essential metals to natural systems. Metal ions play vital roles in many cellular functions such as gene expression/regulation and DNA transcription and repair. The study of metal-protein-DNA/RNA interactions has been relatively unexplored. It is important to understand the role of metalloprotein interactions with DNA/RNA as this enhanced knowledge may lead to better understanding of diseases and therefore more effective treatments. A major milestone in the development of this field was the discovery of the cytotoxic properties of cisplatin in 1965 and its FDA approval in 1978. Since then, two other chemotherapeutic drugs containing platinum, carboplatin and oxaliplatin, have been used in the clinic. These three compounds are all bifunctional with the ligands surrounding platinum In the cis conformation and rearrangement of the ligands to the trans orientation results in a loss of cytotoxic properties due to rapid deactivation through binding to S-containing proteins. This enhanced reactivity yields new opportunities to study the reactions between proteins and DNA. One of the first crosslinking experiments used transplatin to crosslink NCp7 to viral RNA in order to understand how/where the protein bound to RNA. We have studied the interaction between cis and trans dinuclear platinum complexes and the C-terminal zinc finger (ZF). The trans complex reacts at a faster rate than the cis isomer and causes N- terminal specific cleavage of the ZF. The dinuclear structure plays a critical role in the peptide cleavage as studies with transplatin (the mononuclear derivative) does not result in cleavage. Monofunctional trans platinum-nucleobase complexes (MPNs) serve as a model for the binding of transplatin to DNA. This provides an interesting opportunity to study their reactions with S-containing proteins, such as HIV1 NCp7. MPNs have been shown to bind to the C-terminal ZF of HIV1 NCp7, resulting in zinc ejection. This occurs through a two-step process where the nucleobase π-stacks with Trp37 on the ZF, followed by covalent binding at the labile Cl site to Cys. MPNs have also shown antiviral activity in vitro. The labile Cl on MPNs reduces specificity of these compounds, as it leaves an available coordination site on the platinum center for binding to other S-proteins or DNA. Therefore, we have moved to an inert PtN4 coordination sphere, [Pt(dien)L]2+ (dien= diethylenetri- amine). Due to the strong bond between platinum and nitrogen, covalent reactions are highly unlikely to occur at rapid rates. The strength of the pi-stacking interaction between nucleobases (free and platinated) and the aromatic amino acid, tryptophan (Trp), showed an enhanced binding constant for platinated nucleobases. This was confirmed by density functional theory (DFT) calculations as the difference in energy between the HOMO of Trp and the LUMO of the nucleobase was smaller for the platinum complex. The studies were extended to the Trp-containing C-terminal ZF of HIV1 NCp7 and an increase in association constant was seen compared to free Trp. Reaction of PtN4 nucleobases compounds with a short amino acid sequence con- taining either Ala (no pi-stacking capabilities) or Trp (pi-stacking interactions) revealed an enhanced rate of reactivity for the Trp-containing peptide. This result supports the theory of a two-step reaction mechanism where the platinum-nucleobase complex recognizes the pep- tide through a pi-stacking interaction with Trp followed by covalent binding to the platinum center. The [Pt(dien)L]2+ motif allows for systematic modification of the structural elements surrounding platinum in a search for the most effective compound. Methylation of the dien ligand should, in theory, increase lipophilicity of the compounds, however, due to 2+ charge of the compounds, this simple association does not hold true. Analysis of the cellular accumulation profiles showed little change in the uptake with the addition of methyl groups to the dien ligand, in agreement with the non-linear change in lipophilicity. Modification of L using different nucleobases allows for the tuning of the strength of the π-stacking interaction between Trp and the platinum complex. The addition of inosine (which lacks a H-bonding donor/acceptor at the C2 position) resulted in a lower association constant with both N-AcTrp and the C-terminal zinc finger of HIV1 NCp7. Interestingly, the addition of xanthosine resulted in an ehanced pi-stacking interaction with the C-terminal zinc finger of HIV1 NCp7; likely as a results of the addition of a H-bonding donor (double-bonded O) at the C2 position. The ability of PtN4 nucleobase complexes to inhibit formation of the NCp7 complexation with viral RNA was studied by mass spectrometry and gel electrophoresis. Dissociation of the NCp7-RNA complex was seen upon addition of PtN4 compounds. These compounds were also able to retard formation of the NCp7-RNA complex when pre-incubated with the protein. These results have important implications as inhibition of complex formation between NCp7 and viral RNA has negative implications for viral replication. Despite the success of platinum-nucleobase compounds, it is important to evaluate all potential pi-stacking ligands. A series of pyridine- and thiazole-based compounds were evaluated for the strength of the pi-stacking interaction with N-AcTrp and the C-terminal ZF of HIV1 NCp7. There was notable increase in association constant for the platinum- DMAP (4-dimethylaminopyridine) complex compared to other ligands studied. This result highlights the importance of exploring multiple avenues for the design of specifically targeted inhibitors and further confirms the viability of the medicinal chemistry dual approach of target recognition (non-covalent) followed by target fixation (covalent).
49

DNA SELF-ASSEMBLY DRIVEN BY BASE STACKING

Longfei Liu (6581096) 10 June 2019 (has links)
<p>DNA nanotechnology has provided programming construction of various nanostructures at nanometer-level precision over the last three decades. DNA self-assembly is usually implemented by annealing process in bulk solution. In recent several years, a new method thrives by fabricating two-dimensional (2D) nanostructures on solid surfaces. My researches mainly focus on this field, surface-assisted DNA assembly driven by base stacking. I have developed methods to fabricate DNA 2D networks via isothermal assembly on mica surfaces. I have further explored the applications to realize quasicrystal fabrication and nanoparticles (NPs) patterning.</p><p><br></p> <p>In this dissertation, I have developed a strategy to assemble DNA structures with 1 or 2 pair(s) of blunt ends. Such weak interactions cannot hold DNA motifs together in solution. However, with DNA-surface attractions, DNA motifs can assemble into large nanostructures on solid surface. Further studies reveal that the DNA-surface attractions can be controlled by the variety and concentration of cation in the bulk solution. Moreover, DNA nanostructures can be fabricated at very low motif concentrations, at which traditional solution assembly cannot render large nanostructures. Finally, assembly time course is also studied to reveal a superfast process for surface-assisted method compared with solution assembly.</p><p><br></p> <p>Based on this approach, I have extended my research scope from 1D to 2D structures assembled from various DNA motifs. In my studies, I have successfully realized conformational change regulated by DNA-surface interaction and steric effect. By introduction of DNA duplex “bridges” and unpaired nucleotide (nt) spacers, we can control the flexibility/rigidity of DNA nanomotifs, which helps to fabricate more delicate dodecagonal quasicrystals. The key point is to design the length of spacers. For 6-point-star motif, a rigid structure is required so that only 1-nt spacers are added. On the other hand, 3-nt spacers are incorporated to enable an inter-branch angle change from 60° to 90° for a more flexible 5-point-star motif. By tuning the ratio of 5 and 6 -point-star motifs in solution, we can obtain 2D networks from snub square tiling, dodecagonal tiling, a mixture of dodecagonal tiling and triangular tiling, and triangular tiling.</p><p><br></p> Finally, I have explored the applications of my assembly method for patterning NPs. Tetragonal and hexagonal DNA 2D networks have been fabricated on mica surfaces and served as templates. Then modify the surfaces with positively-charged “glues”, <i>e.g.</i> poly-L-lysine (PLL) or Ni<sup>2+</sup>. After that, various NPs have been patterned into designated lattices, including individual DNA nanomotifs, gold NPs (AuNPs), proteins, and silica complexes. Observed NP lattices and fast Fourier Transform (FFT) patterns have demonstrated the DNA networks’ patterning effect on NPs.
50

Dimensional Stacking in Three Dimensions

Walsh, Timothy A. 21 January 2008 (has links)
Dimensional Stacking is a technique for displaying multivariate data in two dimensional screen space. This technique involves the discretization and recursive embedding of dimensions, each resulting N-dimensional bin occupying a unique position on the screen. This thesis describes the extension of this technique to a three dimensional projection. In addition to the visual enhancements, hashing was used to improve the scalability of records and dimensions. The resulting visualization was evaluated by a usability study.

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