Ni80Fe20 and Ni80Fe20/NiO films and nano-stripes were characterized magnetically through AC and DC susceptibility measurements, and hysteresis loops as a function of field and temperature. While the near-pattern films were characterized in the in-plane configuration only, the nano-stripes were characterized in parallel, transverse and the perpendicular field configurations. The effects of the constrained geometry on the coercivity, exchange bias field, and the superparamagnetic blocking temperature were studied. It was determined that the coercivity, exchange bias field and the superparamagnetic blocking temperature can be controlled, not only by using a patterned media instead of a plane film, but also by the orientation of that pattern.

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In this thesis, the concept of style transfer for visual storytelling is introduced. Style transfer for visual storytelling is the process of identifying a definitive style of a source, such as an artist or culture, and applying the features of that style to a target, such as a character which has a different style. As a proof of concept, the style of the American film director Tim Burton is transferred to a character from Hindu mythology, Yamah. The style transfer is done based on the concept of 'Pattern Language' introduced by Christopher Alexander et al., in his book, 'A Pattern Language'. A set of patterns is developed based on the source and target. The target is then designed based on the patterns. The design is then visualized in a suitable medium.

Le cervelet est un petit cerveau dans le cerveau. Il contient plus de la moitié du nombre total de neurones du cerveau. Sa structure très régulière est bien connue, toutefois son rôle demeure mystérieux. Le développement essentiellement postnatal du cervelet chez les rongeurs permet d’y étudier la formation activité-dépendante du réseau de neurones. C’est aussi le siège où s’opèrent diverses formes de plasticité synaptique, ce qui en fait un modèle d’étude idéal pour la plasticité synaptique développementale et adulte. Au cours de cette thèse, à l’aide d’enregistrements électophysiologiques en patch-clamp et en extracellulaire sur des tranches aigües de cervelet de souris et grâce aux techniques immunohistochimiques, j’ai étudié trois acteurs importants de la plasticité synaptique et du développement des cellules de Purkinje, les neurones centraux du cortex cérébelleux. Nous avons démontré que l’activation du récepteur métabotropique glutamatergique de type 1 (mGlu1) déclenche l’activation et l’ouverture de GluD2, un récepteur nécessaire au développement et à la plasticité des synapse des cellules de Purkinje (CPs). Nous avons également mis en évidence que les Pannexines 1, des canaux potentiellement impliqué dans la synchronisation neuronale récemment découverts et encore mal caractérisés, sont exprimées par les cellules de Purkinje Zebrine II –immunopositives, suivant les bandes parasagittales que délimitent les microdomaines du cervelet. Enfin, nous avons étudié la physiologie du cortex cérébelleux des souris néonatales, cherchant à caractériser les différents acteurs essentiels à l’activité neuronale de ce cortex en développement très particulier et peu étudié. L’activation du récepteur GluD2 médiée par mGlu1 dans la synapse entre Fibre Parallèle et cellule de Purkinje (synapse PF-PC). GluD2 est classifié parmi les récepteurs ionotropiques glutamatergique, pourtant aucun ligand n’est capable d’induire l’ouverture de son canal. Nous avons identifié pour la première fois un mécanisme physiologique d’ouverture du canal de GluD2 en démontrant que l’activation de mGlu1 déclenche l’ouverture du canal de GluD2 pour une voie intracellulaire, aussi bien dans un système d’expression en culture que dans les tranches aigues de cervelet murin. Cela nous permettra d’étudier la contribution du courant médié à travers GluD2 dans la plasticité à long terme, avec des perspectives totalement nouvelles. L’expression de Pannexine 1 par les CPs se superpose aux stries Zebrine II- immunopositives du cervelet. Les CPs adultes constituent une population hétérogènes, les différents sous-types étant organisés sur le plan parasagittal. Nous avons montré que l’expression des protéines Pannexine 1 (Panx1) We have shown that Pannexin1 (Panx1) déssine un gradient rostrocaudal discontinu dans les lobules de tranches parasagittales. Sur les coupes coronales, leur distribution forme une série de bandes parasagittales. Les canaux Panx1 médient la libération d’ATP en réponse à divers stimuli et pourrais de cette façon contribuer à une activité neuronale orientée sur le plan parasagittal en réponse aux signaux des fibres parallèles. Caractérisation de l’activité GABAergique des CPs immatures dans les souris néonatales. Le cortex cérébelleux entre les jours postnataux P0 et P4 consistent principalement en une multicouche dense de CPs fortement interconnectées. A cet âge, les CPs sont remplies de GABA extrasynapstique qui est libéré dans le milieu extracellulaire par un mécanisme qui n’est pas clairement identifié. Nos recherches préliminaires sur la première semaine de développment postnatal, nous montrons que l’activation de récepteur au GABA de type A induit une réponse excitatrice chez les CPs. Avec notre préparation, cet effet est indépendant de la présence de corps cétoniques ou de lactate comme substrats énergétiques dans le milieu extracellulaire. (...) / The cerebellum is a little brain in the brain. It houses more than half the total number of neurons in the brain. Its crystalline structure is very well known but, still, its function remain unclear to date. Its mainly postnatal development in rodents allows the study of the physiology of activity-dependent neuronal wiring. It is also the place of many types of neuronal plasticity, making it an ideal model to study both developmental and adult synaptic plasticity. In this thesis, using mainly patch-clamp and extracellular recordings in cerebellar slices as well as immunohistochemistry in mice, I have studied three important actors of synaptic plasticity and development in the Purkinje cells, the principal neurons of the cerebellar cortex. We have established that the type 1metabotropic glutamate receptor (mGlu1) triggers the gating of GluD2, a receptor necessary for Purkinje cells (PCs) synapses development and synaptic plasticity. We have also shown that the Pannexins 1, some channels likely involved in neuronal synchronization that have been recently discovered but yet remain poorly characterized, are expressed by Zebrin II immmunopositive Purkinje cells in the classical Zebra stripes formed by microdomains of the cerebellum. Last, we have studied the physiology of the primitive cerebellar cortex in neonatal mice, establishing the first elements of the neuronal activity of this very particular developing cortex at a stage still very poorly characterized. The mGlu1-mediated gating of Glu2D receptors at Parallel Fiber to PC (PF-PC) synapse. GluD2 are classified among ionotropic glutamate receptors, but no ligand has proved capable of gating their channel. We have identified for the first time a physiological mechanism of gating GluD2 channels by demonstrating that the activation of mGlu1 triggers the opening of GluD2 channels through intracellular pathways, both in expression systems and in acute murine cerebellar slices. This will allow us to study the contribution of GluD2-mediated current in long-term plasticity in a totally new way. Expression of Pannexin1 by PCs matches with adult Zebrin II immunopositive cerebellar stripes. Adult PCs constitute an heterogeneous population, the different subtypes being parasagittaly organized. We have shown that Pannexin1 (Panx1) proteins expression by PCs, draw a rostrocaudal discontinuous gradient in lobules of parasagittal slices. In transverse slices, their distribution forms an array of parasagittal stripes. Panx1 channels mediate ATP release in response to various stimuli and may in this way contribute to parasagittally oriented response to PF inputs. Characterization of GABAergic activity of immature Purkinje cells of newborn mice. The cerebellar cortex during postnatal days P0 to P4 essentially consists in a dense multilayer and highly interconnected network of PCs. At this age, PCs are filled with extrasynaptic GABA which is released in the extracellular space by a mechanism that is not clear. In our preliminary investigation of first week postnatal development, we show that activation of GABA-A receptors leads to excitatory responses in PCs. In our preparation, this effect is independent of the presence of keton bodies or lactate as energetic substrates in the extracellular medium. The complete inhibition of spontaneous discharge of PCs by Panx1 channel blockers, suggests that they mediate ion fluxes or release of neuromediators, such as ATP or GABA.

The diploma thesis deals with construction technological project of Aquacentre Kohoutovice. The thesis handles technological prescription of compact external cladding assembly including supporting structure. The thesis also includes assessment of transport itinerary, itemized budget with bill of quantities, design of site equipment, time and financial plan, construction schedule, machine assembly, control and test plan and safety and health care policy.

The binary of gender is a socially constructed system dividing two opposite sexes, as a result fashion design has required two main fields, womenswear and menswear, and in between them: unisex. In unisex fashion, the male coded garments are favored due to social constructions about gender and norms as well to their loose fitting silhouettes that are used as a solution for being able to fit male and female coded bodies. This work proposes an alternative of what unisex can be through exploring the non-human form of a chair with the purpose to step away from the human body’s biological differences that often dictates fashion design. The method allows for unisex cuts to be explored through the means of experimental pattern cutting, as the gendered body does not dictate the shape of the garment. As a result this work proposes a reaction to the male coded unisex, including female coded materials, decorative shapes allowed to fit many through elasticity. / De binära könsrollerna är ett socialt konstruerat system som delar upp två motsatta kön, vilket resulterat i att modedesign krävt två huvudområden, damkläder och herrkläder, och betraktat som däremellan: unisex. Inom unisex modet gynnas de manligt kodade plaggen på grund av sociala konstruktioner om kön och normer, samt deras löst sittande silhuetter som används som en lösning för att kunna passa både manligt och kvinnligt kodade kroppar. Detta arbete föreslår ett alternativ till vad unisex kan vara genom att utforska den icke-mänskliga formen av en stol med syftet att ta ett steg bort från människokroppens biologiska skillnader som ofta dikterar modedesign. Resultatet föreslår en reaktion på det manligt kodade unisex modet genom att inkludera kvinnligt kodade material, dekorativa former som passar olika kroppar genom sin elasticitet.

The Insurance Bureau of Canada released a report in 2013 that evaluated the seismic risk of two major metropolitan areas of Canada, with projected losses of $75bn in British Columbia along the Cascadia subduction zone, and $63bn in the east through the Ottawa-Montreal-Quebec corridor. Such reports should prompt researchers and designers alike to rethink the way that seismic design is approached in Canada to develop resilient and sustainable cities for the future. To mitigate the economic losses associated with earthquake damage to buildings in seismically active areas, controlled rocking steel braced frames have been developed as a seismically resilient low-damage lateral-force resisting system. Controlled rocking steel braced frames (CRSBFs) mitigate structural damage during earthquakes through a controlled rocking mechanism, where energy dissipation can be provided at the base of the frame, and pre-stressed tendons pull the frame back to its centred position after rocking. The result is a building for which the residual drifts of the system after an earthquake are essentially zero, and the energy dissipation does not result from structural damage. Design methods for the base rocking joint and the capacity-protected frame members in CRSBFs have been proposed and validated both numerically and experimentally. However, the is no consensus on how to approach the design of the frame members, questions remain regarding how best to design CRSBFs to prevent building collapse, and no experimental work has been done regarding how to connect the CRSBF to the rest of the structure to accommodate the rocking motion. Because the force limiting mechanism of a CRSBF is rocking only at the base of the frame, the frame member forces are greatly influenced by the higher-mode response, resulting in more complex methods to design the frame members. This thesis begins by outlining two new design procedures for the frame members in controlled rocking steel braced frames that target both simplicity and accuracy. The first is a dynamic procedure that requires a truncated response spectrum analysis on a model of the frame with modified boundary conditions to consider the rocking behaviour. The second is an equivalent static procedure that does not require any modifications to the elastic frame model, instead using theory-based lateral force distributions to consider the higher modes of the rocking structure. Neither method requires empirical calibration to estimate the forces at the target intensity. The base rocking joint design is generally in good agreement between the various research programs pioneering the development of the CRSBFs. However, the numerous parameters available to select during the design of the base rocking joint give designers an exceptional amount of control over the performance of the system, and little research is available on how best to select these parameters to target or minimise the probability of collapse for the building. This thesis presents a detailed numerical model to capture collapse of buildings with CRSBFs as their primary lateral force resisting system and uses this model to generate collapse fragility curves for different base rocking joint design parameters. The parameters include the response modification factor, the hysteretic energy dissipation ratio, and the post-tensioning prestress ratio. This work demonstrates that CRSBFs are resilient against collapse, as designing the base rocking joint with response modification factors as large as 30, designing the post-tensioning to prevent yielding at moderate seismic hazard levels, and using zero energy dissipation could lead to designs with acceptable margins of safety against collapse. While the design procedures are shown to be accurate for estimating the frame member force demand for the targeted intensity level, there is still a high level of uncertainty around what intensity of earthquake a building will experience during its lifespan, and there is no consensus on what intensity should be targeted for design. To address this, the ability of the capacity design procedures to provide a sufficiently low probability of collapse due to excessive frame member buckling and yielding is evaluated and compared to the probability that the building will collapse due to excessive rocking of the frame. The results of the research presented here suggest that the probability of collapse due to either frame member failure or excessive rocking should be evaluated separately, and that targeting the intensity with a 10% probability of exceedance in 50 years is sufficient for the design of the frame members. Finally, critical to the implementation of CRSBFs in practice is how they may be connected to the rest of the structure to accommodate the uplifting of the CRSBF while rocking under large lateral forces. An experimental program was undertaken to test three proposed connection details to accommodate the relative uplifts and forces. The connections that accommodate the uplifts through sliding performed better than that which accommodated the uplifts though material yielding, but the best way to transfer the forces and accommodate the uplifting without influencing the overall behaviour of the system is to position the connection such that it does not need to undergo large uplifts and carry lateral force simultaneously. A detailed numerical model of the experimental setup is presented and is shown to simulate the important response quantities for each of the tested connections. Using the results of this work, designers worldwide will be confident to design CRSBFs for structures from the base rocking joint to the selection of floor-to-frame connections for a complete system design while ensuring a safe and resilient building structure for public use and well-being. / Thesis / Doctor of Philosophy (PhD) / Traditional approaches to seismic design of buildings have generally been successful at preventing collapse and protecting the lives of the occupants. However, the buildings are often left severely damaged, often beyond repair. To address these concerns, controlled rocking steel braced frames have been proposed as part of a new construction technique to mitigate or prevent damage to steel buildings during earthquakes, but several aspects of the design and overall safety have yet to be explored or demonstrated. This thesis proposes and validates new tools to design controlled rocking steel braced frames and provides recommendations on how best to design them to achieve a safe probability against collapse. Details are proposed and presented for components to connect the controlled rocking steel braced frames into the rest of the structure. The findings of this thesis will aid practitioners looking to deliver resilient and sustainable structural designs for buildings in our cities of the future.

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