Background and Purpose: Dental hygienists are targeted for practice expansion to improve public access to oral health care and, therefore, must demonstrate decision making capacity. This study aimed to identify and test the impact of factors influential in dental hygiene decision making. Organizational and gender factors were hypothesized to be most influential. Methods: A phased mixed methods approach was used. Phase I: A series of focus groups were conducted to inform a dental hygiene decision making model, which included key predictor variables and the outcome variable: decision making capacity. Phase II: Aspects of the model were tested via an electronic questionnaire and key informant interviews. Statistical and qualitative thematic analyses were conducted and then findings were merged for interpretation. Results and Interpretation: Focus groups yielded over 75 codes and 6 themes (+ 1 theme from the literature) comprising the model and guiding the survey. The survey had a 38% response rate, and moderate to weak correlations between predictors and the outcome measure were shown. The final statistical model demonstrated Individual Characteristics and graduating from a 3-year program together significantly predicted decision making capacity. When merged with the key informant qualitative data, Individual Characteristics were shown to be a product of broad environmental factors and educational preparation had a particularly strong influence. Conclusions: Individual characteristics and education are predictive of decision making capacity but are outcomes of broad structural influences. Thus, it is recommended that modifications are made to these structures to support dental hygiene decision making in expanded practice.

This thesis looks at the problem of the differences between the MT Tabernacle accounts and the LXX translation in the Book of Exodus (chs. 25-31 and 35-40). Although the differences between MT Exodus 25-31 and the LXX appear to be a matter of the type of variation which might be expected between source and receptor text with their own history of development, the differences between MT and LXX Exodus 35-40 are far more complex. The order of LXX Exodus 36-39 is quite distinct from that of the MT; the LXX translation is also much shorter than its counterpart. In the past, the general consensus of scholars has been to agree with D.W. Gooding that the arrangement and brevity of LXX Exodus 36-39 were due to the hand of an incompetent translator. After a survey of the problem of the Tabernacle accounts in Chapter one, methodologies are investigated in Chapter two in order to assess the competence (or otherwise) of the translation technique employed by the translator. This methodology is then applied in Chapters three to seven as comparisons are made between 1) the MT Tabernacle accounts; 2) the LXX Tabernacle accounts; 3) the LXX and MT Tabernacle accounts. Since Exodus 29 and 40 do not have a parallel these chapters are studied on their own (Chapters six and seven). The results of each investigation are examined for any clues which may help to solve the problem of major differences or minor discrepancies between the Tabernacle accounts. One difference between this investigation and those previously undertaken, e.g., by Gooding, is that hermeneutical intertextuality plays an important role in discerning the nature of the translation technique of the Tabernacle translator.

A country’s international reputation has profound implications for its citizens; given that national image or reputation is built and circulated using language on a global scale, translation is necessarily involved. This project draws on bilingual corpora of government and media texts to examine how Canada was framed in the discourses and narratives in circulation in its two official languages at the time of the 2010 G8 and G20 Summits, using concepts and techniques from Critical Discourse Analysis, narrative theory, and corpus linguistics. Examining some aspects of language in use such as collocation, semantic relations, and metaphor, several of the ways in which Canada was framed in the two contexts and languages were compared. The project concludes that discourses and narratives may differ between sources and languages, thereby highlighting the importance of recognizing the impact of translation on the variety of national representations within discourses and narratives.

Background and Purpose: Dental hygienists are targeted for practice expansion to improve public access to oral health care and, therefore, must demonstrate decision making capacity. This study aimed to identify and test the impact of factors influential in dental hygiene decision making. Organizational and gender factors were hypothesized to be most influential. Methods: A phased mixed methods approach was used. Phase I: A series of focus groups were conducted to inform a dental hygiene decision making model, which included key predictor variables and the outcome variable: decision making capacity. Phase II: Aspects of the model were tested via an electronic questionnaire and key informant interviews. Statistical and qualitative thematic analyses were conducted and then findings were merged for interpretation. Results and Interpretation: Focus groups yielded over 75 codes and 6 themes (+ 1 theme from the literature) comprising the model and guiding the survey. The survey had a 38% response rate, and moderate to weak correlations between predictors and the outcome measure were shown. The final statistical model demonstrated Individual Characteristics and graduating from a 3-year program together significantly predicted decision making capacity. When merged with the key informant qualitative data, Individual Characteristics were shown to be a product of broad environmental factors and educational preparation had a particularly strong influence. Conclusions: Individual characteristics and education are predictive of decision making capacity but are outcomes of broad structural influences. Thus, it is recommended that modifications are made to these structures to support dental hygiene decision making in expanded practice.

No description available.

Background: Task shifting provision of basic health care services to lay health workers (LHWs) is increasingly employed to combat the global shortage of skilled health workers, particularly in low and middle income countries, where the shortage is greatest. Despite evidence for the effectiveness of LHWs in improving access to basic health services and positively impacting a variety of health outcomes, questions remain as to how recognized weaknesses in training and supervision are best addressed. This thesis employed a sequential-concurrent mixed-methods design and is composed of 3 studies with the objective of designing and rigorously evaluating a knowledge translation (KT) intervention tailored to address identified barriers to LHWs ability to function optimally as TB adherence supporters in Zomba district, Malawi. Methods & Findings: The first study utilized the qualitative methods of focus groups and interviews conducted with LHWs routinely involved in provision of care to TB patients. Lack of TB knowledge and job-specific training were identified as the key barriers to LHWs in their role as TB adherence supporters. Based on these findings, a KT intervention was developed and tailored to the identified training gaps. The second study, evaluated the effectiveness of the intervention in improving TB treatment adherence in a cluster randomized controlled trial, which showed no evidence for effectiveness of the intervention. The third study, conducted concurrently with the cluster trial, employed qualitative interviews to explore LHWs experiences with the intervention to identify ii aspects of the intervention found to be helpful and areas in need of improvement. Study 3 found that the intervention was well received and valued, with reported benefits to LHWs through improved knowledge and skills, and increased confidence. Suggestions for improvement varied considerably, with an anticipated concern with the lack of stipends and conduct of training on-site, raised as an issue by a minority of participants. Conclusion: This thesis suggests that a multi-component KT strategy tailored to address local barriers, was well received and valued by LHWs, and may represent a cost-effective approach to LHW training. However, given the trend for effectiveness did not reach significance in this underpowered study, further research is needed.

The ssrA gene encoded tmRNA acts as both a tRNA carrying an Ala to enter the A site of stalled ribosomes and as an mRNA allowing trans-translation to continue until ribosomes reach the stop codon of the tmRNA tag to help release the stalled ribosome, label the truncated peptide for degradation, and also facilitate degradation of the ribosome-stalling mRNAs. Functions of tmRNA rely on its binding to an essential protein factor SmpB that is encoded by the smpB gene. The mycelial bacteria streptomycetes have a well-defined growth and developmental cycle culminating at sporulation and provide a good model to study tmRNA function in bacteria growth and development. During different developmental stages, expression of some critical molecules are increased or decreased to control the developing procedures including a bldA-encoded tRNA that decodes the rare codon UUA. Translation elongation of genes containing UUA rare codons may be stalled and elicits tmRNA tagging, suggesting that tmRNA the tagging system may be important for Streptomyces growth and development. We use the most well studied strain, S. coelicolor whose genome sequence was the first sequenced, as our model organism. Here I report my ssrA knockout study with two different strategies. Using a temperature sensitive replicon, I found that the ssrA gene could be disrupted only in cells with an extra ssrA gene but not in wild type cells or cells with an extra-copy of tmRNA variant--tmRNADD that encodes a degradation-resistant tag. These results imply that ssrA is an essential gene and that degradation of truncated proteins is also an essential function for S. coelicolor. On the contrary, with the second method that does not need high temperature screening steps I was able to disrupt both the ssrA and smpB genes separately and at the same time, suggesting that the tmRNA tagging system may be required for cell survival under high temperature. Further characterization of mutant cells revealed that the tmRNA tagging system is important for cell growth and development at both high temperature and optimal growth conditions as well as under stress conditions that affect the translation elongation process. The second part of my thesis documents analyses of the expression, regulation and stability of S. coelicolor tmRNA. My results suggested that the well known metabolic stability of bacterial tmRNA might be due to its tight binding to the ribosome. Finally, I report my investigation of the tagging activity and the importance of some structural elements of S. coelicolor tmRNA. Particularly, I demonstrated that pseudoknot 4 is important for tmRNA tagging activity and mutations to some structural elements lead to a decrease of not only the mutant tmRNA but also the wild type tmRNA when expressed together in vivo.

Background: Task shifting provision of basic health care services to lay health workers (LHWs) is increasingly employed to combat the global shortage of skilled health workers, particularly in low and middle income countries, where the shortage is greatest. Despite evidence for the effectiveness of LHWs in improving access to basic health services and positively impacting a variety of health outcomes, questions remain as to how recognized weaknesses in training and supervision are best addressed. This thesis employed a sequential-concurrent mixed-methods design and is composed of 3 studies with the objective of designing and rigorously evaluating a knowledge translation (KT) intervention tailored to address identified barriers to LHWs ability to function optimally as TB adherence supporters in Zomba district, Malawi. Methods & Findings: The first study utilized the qualitative methods of focus groups and interviews conducted with LHWs routinely involved in provision of care to TB patients. Lack of TB knowledge and job-specific training were identified as the key barriers to LHWs in their role as TB adherence supporters. Based on these findings, a KT intervention was developed and tailored to the identified training gaps. The second study, evaluated the effectiveness of the intervention in improving TB treatment adherence in a cluster randomized controlled trial, which showed no evidence for effectiveness of the intervention. The third study, conducted concurrently with the cluster trial, employed qualitative interviews to explore LHWs experiences with the intervention to identify ii aspects of the intervention found to be helpful and areas in need of improvement. Study 3 found that the intervention was well received and valued, with reported benefits to LHWs through improved knowledge and skills, and increased confidence. Suggestions for improvement varied considerably, with an anticipated concern with the lack of stipends and conduct of training on-site, raised as an issue by a minority of participants. Conclusion: This thesis suggests that a multi-component KT strategy tailored to address local barriers, was well received and valued by LHWs, and may represent a cost-effective approach to LHW training. However, given the trend for effectiveness did not reach significance in this underpowered study, further research is needed.

Translational control is an important means of regulating gene expression. Development of the Drosophila germ line relies on translational regulation to differentially express maternal mRNAs, allowing it to develop distinctly from the soma. One of the critical factors required for germ cell development and function is the conserved DEAD-box RNA helicase Vasa (Vas). The research presented in this thesis examines the role of Vas in translational regulation during Drosophila germ line development. A two-hybrid screen conducted with Vas identified a translation initiation factor eIF5B (dIF2), as a direct interactor. Mutations were created in eIF5B and were found to enhance the vas mutant phenotypes of reduced germ cell numbers, and posterior segmentation defects, suggesting a functional interaction between these factors in vivo. In order to further understand the biological significance of the Vas-eIF5B interaction, the region of Vas required for eIF5B-binding was mapped and then specifically disrupted. Reduction of Vas-eIF5B binding using a transgenic approach, virtually eliminated germ cell formation, while having only a moderate effect on the somatic requirement of Vas in posterior segmentation. In addition, Vas-eIF5B interaction was found to be required for the establishment of polarity within the egg during oogenesis, likely through direct regulation of gurken (grk) mRNA. We concluded that through interaction with eIF5B, Vas plays a critical role in translational regulation in the germ line. In addition, another Drosophila DEAD-box protein, highly similar to Vas, called Belle (Bel) was characterized. Mutations in bel were found to also affect the germ line, leading to both female and male sterility. Like Vas, Bel is implicated in translation initiation, however bel is an essential gene, with a requirement for growth, whose function is not restricted to the germ line. Our data suggest that Bel may be a nucleocytoplasmic shuttling protein,

Arabic satellite television has recently attracted tremendous attention in both the academic and professional worlds, with a special interest in Aljazeera as a curious phenomenon in the Arab region. Having made a household name for itself worldwide with the airing of the Bin Laden tapes, Aljazeera has set out to deliberately change the culture of Arabic journalism, as it has been repeatedly stated by its current General Manager Waddah Khanfar, and to shake up the Arab society by raising awareness to issues never discussed on television before and challenging long-established social and cultural values and norms while promoting, as it claims, Arab issues from a presumably Arab perspective. Working within the meta-frame of democracy, this Qatari-based network station has been received with mixed reactions ranging from complete support to utter rejection in both the west and the Arab world. This research examines the social semiotics of Arabic television and the socio-cultural impact of translation-mediated news in Arabic satellite television, with the aim to carry out a qualitative content analysis, informed by framing theory, critical linguistic analysis, social semiotics and translation theory, within a re-mediation framework which rests on the assumption that a medium “appropriates the techniques, forms and social significance of other media and attempts to rival or refashion them in the name of the real" (Bolter and Grusin, 2000: 66). This is a multilayered research into how translation operates at two different yet interwoven levels: translation proper, that is the rendition of discourse from one language into another at the text level, and translation as a broader process of interpretation of social behaviour that is driven by linguistic and cultural forms of another medium resulting in new social signs generated from source meaning reproduced as target meaning that is bound to be different in many respects. The research primarily focuses on the news media, news making and reporting at Arabic satellite television and looks at translation as a reframing process of news stories in terms of content and cultural values. This notion is based on the premise that by its very nature, news reporting is a framing process, which involves a reconstruction of reality into actualities in presenting the news and providing the context for it. In other words, the mediation of perceived reality through a media form, such as television, actually modifies the mind’s ordering and internal representation of the reality that is presented. The research examines the process of reframing through translation news already framed or actualized in another language and argues that in submitting framed news reports to the translation process several alterations take place, driven by the linguistic and cultural constraints and shaped by the context in which the content is presented. These alterations, which involve recontextualizations, may be intentional or unintentional, motivated or unmotivated. Generally, they are the product of lack of awareness of the dynamics and intricacies of turning a message from one language form into another. More specifically, they are the result of a synthesis process that consciously or subconsciously conforms to editorial policy and cultural interpretive frameworks. In either case, the original message is reproduced and the news is reframed. For the case study, this research examines news broadcasts by the now world-renowned Arabic satellite television station Aljazeera, and to a lesser extent the Lebanese Broadcasting Corporation (LBC) and Al- Arabiya where access is feasible, for comparison and crosschecking purposes. As a new phenomenon in the Arab world, Arabic satellite television, especially 24-hour news and current affairs, provides an interesting area worthy of study, not only for its immediate socio-cultural and professional and ethical implications for the Arabic media in particular, but also for news and current affairs production in the western media that rely on foreign language sources and translation mediation for international stories.