There are many issues and challenges to deal with when designing a curved I-girder bridge. These challenges primarily deal with the many performance stages that curved I-girder bridges have such as the erection, construction, and in-service stages. When design engineers assess the stability of a bridge system, they typically evaluate the system in its final configuration with all cross frames attached and the hardened concrete deck placed. The evaluation of girder stability during erection and early stages of construction stages is difficult because of the limited presence of bracing in the system. Due to a lack of readily available analytical tools, many contractors do not conduct detailed analytical evaluations of the bridge behavior during early stages of the construction when stability is often critical. Instead, many contractors use rules of thumb and experience to ensure stability during erection. Erection and construction practices typically vary among contractors and consistent erection methods are a rarity. Although some rules of thumb may be quite conservative, others are much less so. Therefore, coming up with design guidelines based on parametric studies rather than rules of thumb are desirable to help allow the contractor and the designer to work together to prevent issues that may occur due to the lack of communication between the two professions. Lastly, many challenges arise due to the complex geometry of curved I-girders. To prevent excessive rotation in erected girders, three points of vertical support are often provided. Two of these points usually consist of permanent supports in the form of bridge piers or abutments. The third point of support may consist of a temporary support in the form of a shore tower or holding crane. Cases where a holding crane may be satisfactory over a shore tower are also not well understood. To improve the understanding of lifting practices and temporary support requirements, parametric studies were conducted using the finite element program ANSYS. Field data consisting of displacement, stress, and girder rotations gathered from two tests were used to validate both the linear and geometric non-linear three-dimensional FEA models. Upon validation, the finite element model was used to conduct linear and geometric non-linear analyses to determine critical factors in curved I-girder bridges during construction. Specifically, serviceability limit states were studied for the lifting of curved girders. For partially constructed states, parametric studies were conducted to determine optimal locations to place temporary supports as well as to investigate stability differences between using a shore tower and a holding crane. Recommendations are presented to provide guidance for the lifting of curved I-girders as well as to maximize stability of partially constructed bridges. / text

[pt] A presente tese trata de técnicas de baixo custo econômico, acessíveis à população e adequadas ao emprego de materiais que possam ser utilizados em seu estado natural, com pouco beneficiamento. O bambu, a terra crua e as fibras e resinas vegetais, são basicamente os materiais utilizados. Primeiramente foram realizados e testados objetos experimentais para funcionar como elementos de vedações, de cobertura, de sobrecobertura, de proteção externa – brise-soleil e arcos estruturais. Em seguida descreve o aperfeiçoamento de processos de encapsulamento do bambu em desenvolvimento no Laboratório de Investigação Livre Desenho da PUC-Rio. Dando continuidade ao trabalho sobre essas técnicas construtivas, a partir da manipulação de uma determinada bolha de sabão, foi elaborada uma série de modelos experimentais mecânicos e virtuais para uma sala multiuso de 7,70m x 6,00m de base, com altura de 3,20m, feita de fitas de bambu, mantas de barro cru, fibras e resinas vegetais. Na geometria destes modelos, paredes e cobertura se fundem em uma superfície contínua, com duas camadas superpostas, espaçadas e interligadas, formando uma casca que se apoia em toda a extensão do seu perímetro. A seguir, foi feita uma construção em escala real em que fitas de bambu descreviam a geometria base da bolha para testarmos de maneira não instrumentalizada possibilidades de montagem e desmontagem e os índices de resistência devidos ao particular formato da bolha. Finalmente, baseado neste último experimento, são apresentadas soluções gerais inovadoras para o conforto higrotérmico em climas tropicais úmidos que substituem a estratégia da inércia térmica dos materiais espessos e pesados pela leveza de uma dupla casca estrutural que abriga câmaras de ar ventiladas em seu interior. / [en] This thesis studies low cost building techniques that are accessible to a wide range of population groups and are suitable for the use of materials in their natural state with little or no processing. The materials used are basically bamboo, raw earth and vegetable fibers and resins. First a series of tests were carried out with experimental objects to assess suitability for use as sealants, covering, outer covering and external protection – brise –soleil panels and structural arches. Following this, we describe the perfecting of the bamboo coating process in progress the Free Design Laboratory of Rio’s Catholic University. Building on these construction techniques, inspired by the manipulation of a soap bubble a series of experimental mechanical and virtual models were elaborated for a multi-use space measuring 7.7m by 6m at the base with a height of 3.2m, made of bamboo tapes, with a raw earth, fiber and resin covering. In the geometry of these models the walls and covering are a continuous surface with two layers one on another, separated but interconnected. In this way the outer wall is formed which extends over and is supported by the entire perimeter of the structure. The following step was to make a full scale construction in which bamboo tapes were used to form the geometric shape of the base of the bubble in order to do practical tests of the possibilities of assembly and disassembly of such a structure. Tests were also done on the resistance factors afforded by the singular bubble format. Finally, based on the previous experiment we present innovative solutions to the problem of hygrothermic comfort in humid tropical climates which substitute the thermal inertia strategy of thick, heavy building materials with the lightness of a double outer layer that holds ventilated air chambers between these layers.

INTRODUÇÃO: O aracnídeo Phoneutria nigriventer, também conhecido por aranha-armadeira, possui um veneno complexo, contendo vários peptídeos que ativam canais iônicos nas células. Dentre estes, só dois neuropeptídeos, Tx2-5 e Tx2-6, destacam-se por relaxar o músculo liso trabecular do corpo cavernoso, induzindo ereção peniana em camundongos e ratos. Este efeito tem sido associado à produção de oxido nítrico pela ativação de óxido nítrico sintases. No entanto, faltam estudos mais amplos para determinar o papel de Tx2-6 na indução da ereção. OBJETIVOS: Identificar os genes diferencialmente expressos no tecido erétil de camundongos após indução da ereção pela Tx2-6 utilizando microarranjos de oligonucleotídeos. Validação dos resultados obtidos nos microarranjos por PCR quantitativa e imuno-histoquímica. MATERIAIS E MÉTODOS: Camundongos machos e adultos da linhagem Swiss foram divididos em dois grupos: controle (n=10), inoculados pela via intracavernosa com 20 l de solução salina; e tratado (n=10), os quais receberam 0,006gg/animal do peptídeo Tx2-6 diluído em 20 l de salina pela via intracavernosa. Uma hora após o início da ereção no grupo tratado todos os animais foram sacrificados e retirou-se o pênis. Este último foi dividido em dois fragmentos, uma parte do material foi congelada em nitrogênio líquido e mantida a 80°C até a extração do RNA para os experimentos de microarranjos e PCR quantitativa; outra parte foi utilizada para avaliação imuno-histoquímica. RESULTADOS: No grupo tratado a ereção foi observada 30-45 minutos após aplicação de Tx2-6 e mantida durante 120 minutos. Os camundongos de grupo controle não apresentaram nenhum indício de ereção. Nos experimentos de microarranjos, onde foram analisados 34.000 genes representando o genoma total do camundongo, identificou-se 3.803 (12,3%) genes com expressão diferencial de pelo menos ±1,5 vez entre os grupos (1.823 genes superexpressos e 1.980 genes subexpressos no grupo tratado comparado ao controle). Os genes ednrb, sparc, fn1, sstr2, pdgfr foram selecionados para validação dos microarranjos por PCR quantitativa e confirmaram a superexpressão em relação aos controles. As proteínas Fn1, Sstr2 e Pdgfr resultaram aumentadas no grupo tratado após avaliação imuno-histoquímica. CONCLUSÕES: A inoculação de Tx2-6 pela via intracavernosa alterou o perfil de expressão gênica no tecido erétil de camundongos. O número de genes superexpressos foi similar ao de genes subexpressos. Serão necessários outros estudos para entender melhor as vias moleculares que Tx2-6 afeta na indução da ereção peniana. / INTRODUCTION: The Phoneutria nigriventer arachnid, also known as armed-spider, has a complex venom, composed by several peptides that affect cellular ionic channels. Among these, only two neuropeptides, Tx2-5 and Tx2-6 induce penile erection in mice and rats and this effect has been associated with the production of nitric oxide by the activation of nitric oxide synthases. Moreover, there is a scarcity of studies focusing on the role of Tx2-6 in the induction of erection. OBJECTIVES: To identify the differently expressed genes in the erectile tissue of mice after erection induction by Tx2-6 using oligonucleotide microarrays. To validate microarray results by quantitative PCR and immunohistochemistry. MATERIALS AND METHODS: Swiss adult male mice were divided in two groups: control (n=10) were injected intracavernously with 20 gl of saline solution; and treated (n=10) were injected intracavernously with 0.006gg/mouse of the Tx2-6 peptide diluted in 20 gl of saline solution. After checking the penile erection in the treated group, all mice were sacrificed one hour after the beginning of erection for the removal of the penis. Penile organ was divided into two fragments, one piece was immediately frozen in liquid-nitrogen and stored at -80°C until RNA extraction to make the microarray and quantitative PCR experiments; the other was reserved for immunohistochemistry analysis. RESULTS: In the treated group, erection was noticed 30-45 minutes after Tx2-6 inoculation and lasted for 120 minutes. Control mice did not present any sign of erection. Considering as differentially expressed genes with a ±1.5 fold expression difference, of the 34,000 genes on the microarray we identified 3,803 (12.3%) genes differentially expressed between the groups (1,823 genes up-regulated and 1,980 genes down-regulated in the treated group compared to controls). The ednrb, sparc, fn1, sstr2, pdgfr genes were selected for validation of microarray results by using quantitative PCR and confirmed the up-regulation when compared to controls. After immunohistochemistry analysis the Fn1, Sstr2 and Pdgfr proteins were found increased in the treated group. CONCLUSIONS: The intracavernous inoculation of Tx2-6 modified the gene expression profile of erectile tissue of mice. The number of upregulated and down-regulated genes was similar. Further studies are needed to understand the molecular pathways that Tx2-6 affect to induce penile erection.

This dissertation titled The History of the Catholic Church in Lesotho 1862-1989 has three chapters. The first chapter deals with the formation of the seventy-seven missions in Lesotho, and the establishment of the four dioceses of Lesotho the seventy-seven mission had all been formed during the period mentioned in the title, and those formed after appear in the appendix. The second chapter deals with the socio-political involvement of the Catholic Church in Lesotho, this part deals with the authority of the bishop and the Church government and again with the role of the bishop and his relationship to the Oblate authority. In this part four bishops are taken to illustrate this point, and this covers the period from 1930 to 1966. In short this deals with the internal affairs of the Church, and its finances. And we go on to deal with the involvement of the Church in the party politics which led to independence of the country. The last chapter deals with the church under the indigenous clergy, which actually is inculturation its problem and attempted solution. / Christianity, Spirituality, Church History and Missiology / M. Th. (Church History)

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DISSERTAÇÃO- JOSEPHA KARINNE DE OLIVEIRA FERRO.pdf: 4408522 bytes, checksum: 7dd07f574b076880fbd1fc80fd506744 (MD5) / Item reinstated by Fabio Sobreira Campos da Costa (fabio.sobreira@ufpe.br) on 2017-03-09T12:54:13Z Item was in collections: Dissertações de Mestrado - Fisioterapia (ID: 151) No. of bitstreams: 2 license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) 1. DISSERTAÇÃO- JOSEPHA KARINNE DE OLIVEIRA FERRO.pdf: 4408522 bytes, checksum: 7dd07f574b076880fbd1fc80fd506744 (MD5) / CAPES / Além das perdas motoras e sensitivas, o trato urinário e a função sexual são também afetados pela lesão medular, sendo a disfunção sexual um dos problemas mais comuns nestes pacientes e sua gravidade depende no nível e da complexidade da lesão. A alteração da função sexual acontece devido às mudanças no processo neurofisiológico, mas fatores psicossociais podem estar associados à disfunção. A perda da realização sexual, quando comparada à perda de autonomia, pode parecer uma consequência minoritária da lesão medular. No entanto, a função sexual é um importante componente de saúde, com evidências de que a redução da função e satisfação sexual resultam em baixa qualidade de vida, além de um fator fundamental na motivação, bem estar e satisfação. Apesar da relevância, o desempenho sexual após lesão medular ainda é um tema pouco abordado e bastante negligenciado durante o processo de reabilitação, além de serem escassos os estudos que verificam associações mais aprofundadas sobre a função erétil e os demais componentes da função sexual, fatores explicativos e preditivos de disfunção. OBJETIVO: avaliar a função sexual de homens com lesão medular traumática e analisar a associação da disfunção com fatores fisiológicos e não fisiológicos. MATERIAIS E MÉTODOS: Estudo observacional (CCAE 41221414.5.0000.5208) realizado com 45 homens, faixa etária entre 18 a 60 anos, com lesão medular traumática e vida sexual ativa. A função sexual foi avaliada pelo Índice Internacional de Função Erétil (IIFE) e nível e grau da lesão foram determinados seguindo as diretrizes da International Standards for Neurological Examination and Functional Classification of Spinal Cord Injury. Os dados foram coletados após seis meses da lesão, em hospitais de referência. Foram aplicadas técnicas de estatística descritiva e análise bivariada e multivariada, através de regressão logística ajustada para observar a associação entre a função sexual e fatores explicativos de disfunção, com nível de significância de 0,05. RESULTADOS: Participaram do estudo 45 indivíduos com idades entre 18 e 56 anos (média 34,0; IC 31,5 – 37,1) e tempo de lesão médio de 7,5 anos (IC 5,2 – 9,9). Lesões incompletas acima do segmento medular L2 foram as mais frequentes (66,7%). Ao analisar os domínios do IIFE, não foi observada associação entre disfunção sexual e complexidade da lesão, porém, os pacientes com lesões completas foram os que apresentaram mais grave disfunção orgástica (86,7%) e de satisfação (86,7%). Ao analisar a associação entre fatores explicativos para disfunção, percebeu-se que a presença parceira fixa é um fator protetor (OR: 0,22; IC95%:0,05-0,92) para disfunção erétil. Desejo sexual tem associação com parceira fixa (OR: 0,20; IC95%:0,04-0,84), masturbação (OR: 0,16; IC95%:0,04-0,67) e relação sexual no último mês (OR: 0,13; IC95%:0,01-0,92). Ejaculação presente (OR: 0,01; IC95%:0,00-0,15) foi considerado um fator protetor para disfunção orgástica, enquanto disfunção erétil (OR: 15,7; IC95%:1,38-178,58), um fator de risco. Ereção psicogênica (OR: 0,07; IC95%:0,01-0,69), frequência mensal de relação sexual (OR: 11,3; IC95%:2,0-62,8) e disfunção orgástica (OR: 7,1; IC95%:1,1-44,8) tem associação com satisfação. CONCLUSÃO: a resposta sexual altera-se após a lesão medular, tendo a função orgástica como a mais acometida, caracterizando o domínio que apresentou maior e mais grave disfunção. Fatores como presença de parceira fixa, ejaculação e masturbação são fatores de proteção para disfunção sexual. Disfunção erétil, orgástica e relações sexuais infrequentes são preditores de disfunção. / In addition to the motor and sensory loss, urinary tract and sexual function are also affected by spinal cord injury, and sexual dysfunction one of the most common problems in these patients and its severity depends on the level and complexity of the injury. The change in sexual function occurs due to changes in neurophysiological process, but psychosocial factors may be associated with dysfunction. The loss of sexual fulfillment, compared to a loss of autonomy, it may seem a minor consequence of spinal cord injury. However, sexual function is a major health component, with evidence that the reduction of sexual function and satisfaction resulting in lower quality of life, and a key factor in motivation and satisfaction welfare. Despite the relevance, sexual performance after spinal cord injury is still somewhat topic and largely neglected during the rehabilitation process, and few studies that verify deeper associations of erectile function and other sexual function, explanatory factors and predictive dysfunction. AIMS: To evaluate the sexual function of men with spinal cord injury and to analyze the association of the disorder with physiological and non-physiological factors. MATERIALS AND METHODS: Observational study (CEAC 41221414.5.0000.5208) conducted with 45 men, aged 18 to 60 years, with spinal cord injury and active sex life. Sexual function was assessed by the International Index of Erectile Function (IIEF) and level and degree of injury were determined following the guidelines of the International Standards for Neurological and Functional Examination Classi fi cation of Spinal Cord Injury. Data were collected after six months of injury in referral hospitals. Descriptive statistical techniques were applied and bivariate and multivariate analysis using logistic regression adjusted to observe the association between sexual function and explanatory factors of dysfunction, with a 0.05 significance level. RESULTS: The study included 45 subjects aged between 18 and 56 years, mean 34.0 (CI 31.5 to 37.1) and average injury time in years 7.5 (CI 5.2 to 9.9). incomplete lesions above the spinal segment L2 were the most common (66.7%). By analyzing the domains of IIEF, there was no association between sexual dysfunction and complexity of the injury, however, patients with complete injuries were those with more severe orgasmic dysfunction (86.7%) and satisfaction (86.7%). When analyzing the association between explanatory factors for dysfunction, it was realized that a primary partner presence is a protective factor (OR: 0.22; 95% CI: .05-.92) for erectile dysfunction. sexual desire is associated with a steady partner (OR: 0.20; 95% CI: 0.04 to 0.84), masturbation (OR: 0.16; 95% CI: 0.04 to 0.67) and sexual intercourse in the last month (OR: 0.13; 95% CI: 0.01 to 0.92). This ejaculation (OR: 0.01; 95% CI: .00-.15) was considered a protective factor for orgasmic dysfunction as erectile dysfunction (OR: 15.7; 95% CI: 1.38 to 178.58), a risk factor. psychogenic erection (OR: 0.07; 95% CI: 0.01 to 0.69), monthly frequency of intercourse (OR: 11.3; 95% CI: 2.0 to 62.8) and orgasmic dysfunction (OR: 7.1; 95% CI: 1.1 to 44.8) is associated with satisfaction. CONCLUSION: the sexual response changes after spinal cord injury, and orgasmic function as the most affected, featuring the area with the highest and most severe dysfunction. Factors such as the presence of fixed partner, ejaculation and masturbation are protective factors for sexual dysfunction. erectile dysfunction, orgasmic and infrequent sex dysfunction are predictors.

INTRODUÇÃO: O aracnídeo Phoneutria nigriventer, também conhecido por aranha-armadeira, possui um veneno complexo, contendo vários peptídeos que ativam canais iônicos nas células. Dentre estes, só dois neuropeptídeos, Tx2-5 e Tx2-6, destacam-se por relaxar o músculo liso trabecular do corpo cavernoso, induzindo ereção peniana em camundongos e ratos. Este efeito tem sido associado à produção de oxido nítrico pela ativação de óxido nítrico sintases. No entanto, faltam estudos mais amplos para determinar o papel de Tx2-6 na indução da ereção. OBJETIVOS: Identificar os genes diferencialmente expressos no tecido erétil de camundongos após indução da ereção pela Tx2-6 utilizando microarranjos de oligonucleotídeos. Validação dos resultados obtidos nos microarranjos por PCR quantitativa e imuno-histoquímica. MATERIAIS E MÉTODOS: Camundongos machos e adultos da linhagem Swiss foram divididos em dois grupos: controle (n=10), inoculados pela via intracavernosa com 20 l de solução salina; e tratado (n=10), os quais receberam 0,006gg/animal do peptídeo Tx2-6 diluído em 20 l de salina pela via intracavernosa. Uma hora após o início da ereção no grupo tratado todos os animais foram sacrificados e retirou-se o pênis. Este último foi dividido em dois fragmentos, uma parte do material foi congelada em nitrogênio líquido e mantida a 80°C até a extração do RNA para os experimentos de microarranjos e PCR quantitativa; outra parte foi utilizada para avaliação imuno-histoquímica. RESULTADOS: No grupo tratado a ereção foi observada 30-45 minutos após aplicação de Tx2-6 e mantida durante 120 minutos. Os camundongos de grupo controle não apresentaram nenhum indício de ereção. Nos experimentos de microarranjos, onde foram analisados 34.000 genes representando o genoma total do camundongo, identificou-se 3.803 (12,3%) genes com expressão diferencial de pelo menos ±1,5 vez entre os grupos (1.823 genes superexpressos e 1.980 genes subexpressos no grupo tratado comparado ao controle). Os genes ednrb, sparc, fn1, sstr2, pdgfr foram selecionados para validação dos microarranjos por PCR quantitativa e confirmaram a superexpressão em relação aos controles. As proteínas Fn1, Sstr2 e Pdgfr resultaram aumentadas no grupo tratado após avaliação imuno-histoquímica. CONCLUSÕES: A inoculação de Tx2-6 pela via intracavernosa alterou o perfil de expressão gênica no tecido erétil de camundongos. O número de genes superexpressos foi similar ao de genes subexpressos. Serão necessários outros estudos para entender melhor as vias moleculares que Tx2-6 afeta na indução da ereção peniana. / INTRODUCTION: The Phoneutria nigriventer arachnid, also known as armed-spider, has a complex venom, composed by several peptides that affect cellular ionic channels. Among these, only two neuropeptides, Tx2-5 and Tx2-6 induce penile erection in mice and rats and this effect has been associated with the production of nitric oxide by the activation of nitric oxide synthases. Moreover, there is a scarcity of studies focusing on the role of Tx2-6 in the induction of erection. OBJECTIVES: To identify the differently expressed genes in the erectile tissue of mice after erection induction by Tx2-6 using oligonucleotide microarrays. To validate microarray results by quantitative PCR and immunohistochemistry. MATERIALS AND METHODS: Swiss adult male mice were divided in two groups: control (n=10) were injected intracavernously with 20 gl of saline solution; and treated (n=10) were injected intracavernously with 0.006gg/mouse of the Tx2-6 peptide diluted in 20 gl of saline solution. After checking the penile erection in the treated group, all mice were sacrificed one hour after the beginning of erection for the removal of the penis. Penile organ was divided into two fragments, one piece was immediately frozen in liquid-nitrogen and stored at -80°C until RNA extraction to make the microarray and quantitative PCR experiments; the other was reserved for immunohistochemistry analysis. RESULTS: In the treated group, erection was noticed 30-45 minutes after Tx2-6 inoculation and lasted for 120 minutes. Control mice did not present any sign of erection. Considering as differentially expressed genes with a ±1.5 fold expression difference, of the 34,000 genes on the microarray we identified 3,803 (12.3%) genes differentially expressed between the groups (1,823 genes up-regulated and 1,980 genes down-regulated in the treated group compared to controls). The ednrb, sparc, fn1, sstr2, pdgfr genes were selected for validation of microarray results by using quantitative PCR and confirmed the up-regulation when compared to controls. After immunohistochemistry analysis the Fn1, Sstr2 and Pdgfr proteins were found increased in the treated group. CONCLUSIONS: The intracavernous inoculation of Tx2-6 modified the gene expression profile of erectile tissue of mice. The number of upregulated and down-regulated genes was similar. Further studies are needed to understand the molecular pathways that Tx2-6 affect to induce penile erection.

Orientador: Gilberto de Nucci / Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas / Made available in DSpace on 2018-08-26T11:10:20Z (GMT). No. of bitstreams: 1 Arruda_AndreMoreiraMartins_M.pdf: 11172054 bytes, checksum: db7fb87cc200cb091d3be4733e8d8af5 (MD5) Previous issue date: 2013 / Resumo: A presença de um par de órgãos copuladores, os hemipênis, é a característica mais singular do grupo Squamata, que reúne as serpentes e os lagartos. Para que ocorra a ereção, o hemipênis sofre ingurgitamento dos corpos cavernosos por sangue e linfa, além de contar com o auxílio da contração do músculo propulsor do pênis e o relaxamento do músculo retrator. O coito nestes animais pode durar até 28 horas, porém, os mecanismos envolvidos, as estruturas e sua base farmacológica de funcionamento são ainda pouco conhecidas. O hemipênis consiste de dois corpos cavernosos funcionalmente concêntricos, um deles contendo feixes de fibras musculares lisas. Em mamíferos, sintases de NO neuronais e endoteliais estão presentes em estruturas neurais e no endotélio, respectivamente, enquanto a guanilato ciclase solúvel e PDE5 (fosfodiesterase tipo 5) estão expressas no músculo liso trabecular. Partindo disto, para investigar as vias presentes no tecido das cobras, foram construídas curvas concentração-resposta cumulativas de relaxamento para a acetilcolina (ACh), nitroprussiato de sódio (SNP), BAY41-2272 e tadalafil em corpos cavernosos de Crotalus (CCC) pré-contraídos com fenilefrina. Relaxamentos induzidos por estímulo elétrico (EFS) também foram feitos na ausência e presença de L-NAME (100 mm), ODQ (10 mM) e tetrodotoxina (TTX, 1 mM). Em CCC pré-contraídos, o relaxamento dependente de frequência, gerado por EFS, durou três vezes mais do que aqueles em CC mamíferos. Embora estes relaxamentos sejam praticamente abolidos por L-NAME ou ODQ, eles não foram afetados pela TTX. Em contraste, o EFS promoveu relaxamento em corpos cavernosos de sagui que haviam sido incubados com TTX / Abstract: The presence of a pair of copulatory organs, the hemipenes, is the most unique feature of the group Squamata, which includes snakes and lizards. For an erection to occur, the hemipenes suffer engorgement of the corpora cavernosa with blood and lymph, besides counting with the aid of contraction of the propellant muscle and relaxation of penis retractor muscle. Coitus in these animals can last up to 28 hours, however, the mechanisms involved, the structures and their pharmacological basis are still little known. The hemipenis consists of two concentric functionally cavernous bodies, one containing bundles of smooth muscle fibers. In mammals, neuronal NO synthases and endothelial cells are present in the endothelium and neuronal structures, respectively, whereas the soluble guanylate cyclase and PDE5 (phosphodiesterase type 5) are expressed in trabecular smooth muscle. To investigas the tissue were constructed cumulative concentration-response curves for relaxation to acetylcholine (Ach), sodium nitroprusside (SNP), BAY41-2272 and tadalafil in the corpora cavernosa of Crotalus (CCC) pre contracted with phenylephrine. Relaxations induced by electrical stimulation (EFS) was also tested in the presence and absence of L-NAME (100 mm), ODQ (10 mM) and tetrodotoxin (TTX, 1 mM). In precontracted CCC, dependent relaxation frequency generated by EFS last three-times more than those in DC mammals. Although these relaxations are virtually abolished by L-NAME or ODQ, they were not affected by TTX. In contrast, EFS caused a relaxation of the corpus cavernosum in marmosets that had been incubated with TTX / Mestrado / Farmacologia / Mestra em Farmacologia

The diploma thesis deals with alternative design of bridge on highway D1 near Považská Bystrica. Bridge is designed as composite steel-concrete bridge with 6 spans in length 35,7m + 42m + 42m + 42m + 42m + 35,7m. Overall length of superstructure of the bridge is 241m. Construction material used in design is steel S355, concrete C35/45 and reinforcing steel bars are grade B500B. Calculation of superstructure of two variants was performed in preliminary design. First variant is twin-girder structure system and box-girder structure system is second variant. Selected twin-girder bridge was developed in more complex structural design report. Direct erection in place with temporary piers was selected as assembly method of steel structure. Slab concreting will be done by mobile formwork. The design and calculation of the bridge structure was carried out according to valid European design codes. The diploma thesis includes engineering report, structural design report of variants, structural design report of final variant, bill of quantities of assembly parts and drawing documentation.

Estruturas em concreto pré-moldado vêm sendo utilizadas com freqüência em sistemas reticulados do tipo multi-piso, destinados às mais variadas atividades como: estabelecimentos comerciais, estacionamentos, escolas, hospitais e etc. A utilização de ligações viga-pilar do tipo semi-rígida constitui uma alternativa interessante para o enrijecimento e para a garantia da estabilidade global deste tipo de estrutura. Atualmente, a rigidez e a resistência destas ligações têm sido determinadas por ensaios de modelos em escala real. Estes ensaios, além de apresentarem um custo elevado, têm aplicação restrita à ligação examinada o que torna esta metodologia limitada e não adequada à prática de elaboração de projeto. Apresenta-se, neste trabalho, um modelo mecânico para determinação numérica da relação força-deslocamento de uma ligação viga-pilar semi-rígida a partir da contribuição da rigidez individual de cada componente de transferência de força utilizado na vinculação. A formulação do equilíbrio do modelo é implementada em planilha eletrônica constituindo-se numa ferramenta de cálculo para o projetista, permitindo o estudo, de forma rápida e amigável, da influência do posicionamento e da rigidez individual de cada componente de transferência de força na rigidez e na resistência da ligação. Este modelo mecânico foi utilizado para o cálculo da rigidez à rotação para momento fletor positivo de uma ligação viga-pilar ensaiada em laboratório. Os valores para a rigidez da ligação obtidos em ensaio e pelo modelo mecânico ficaram muito próximos. Da formulação do equilíbrio do modelo mecânico pode-se extrair ainda uma matriz de rigidez que é utilizada na representação da ligação semi-rígida no modelo de barra da estrutura. Um programa computacional foi desenvolvido para a análise de pórticos planos com ligação viga-pilar semi-rígida considerando ainda as não linearidades físicas e geométricas do modelo de cálculo. O programa foi validado pela comparação de resultados de exemplos também processados no programa ANSYS. A não-linearidade geométrica é considerada pelo método modal, não incremental-iterativo, e que obtém a parcela não-linear da resposta estrutural pela combinação dos seus modos de flambagem. A não-linearidade física do concreto é considerada pelo método da rigidez secante no qual a rigidez de cada barra da estrutura é reduzida na análise conforme suas armaduras e o nível de sua solicitação. Para o cálculo da rigidez secante dos pilares desenvolveu-se um programa que permite a consideração de armaduras ativas e passivas na seção. A análise de alguns exemplos revelou que a protensão dos pilares é também uma alternativa interessante para o enrijecimento da estrutura. Além de exemplos práticos, outros temas pertinentes ao projeto deste tipo de estrutura como: estabilidade na fase construtiva, esforços finais após a montagem, efeitos dependentes do tempo, assimetria de rigidez e plastificação das ligações, são também abordados no trabalho. / Precast concrete multi-storey structures have been more frequently used to construct commercial buildings, parking, schools and hospitals. The use of semi-rigid beam-tocolumn connections is a good strategy to guarantee the global stability of this type of structure. Actually the rigidity and the strength of this type of connection are obtained in an experimental program using full scale prototypes. This procedure is not only expensive but limited and not adequate for design since the results are only valid for the examined connection. This research presents a mechanical model used to determine numerically the force-displacement relationship of a beam-to-column semirigid connection. The procedure uses the contribution of any individual force transfer component active in the connection. The equilibrium equation of the model is implemented in an electronic-worksheet where the designer can rapidly and friendly study the influence of the position and the individual rigidity of each force transfer component in the global rigidity and strength of the connection. For validation, this procedure was used to calculate the flexural rigidity for positive bending moment of a beam-to-column semi-rigid connection that was tested in laboratory. The numerical values of the connection rigidity obtained by test and by the mechanical model are very close. The matrix used in the formulation of the mechanical model equilibrium can also be used to represent the semi-rigid connection in the finite element model of the structure. A computational program was developed for plane frame analysis including semi-rigid beam-to-column connections and both physical and geometric nonlinearities. The program was tested by comparing the results of some examples that are also analysed in the ANSYS program. The geometric nonlinearity is considered by a modal method where the nonlinear response of the structure is obtained by a combination of its buckling modes. The physical nonlinearity of the concrete is considered by the secant rigidity method. In this method the rigidities of all concrete bars in the structure are reduced by coefficients that depend of the reinforcement in the cross section and the level of the efforts. A special computational program was developed to calculate the secant rigidity for a concrete cross section with reinforcing bar and prestressing steel. The analysis of some examples shows that the use of prestressing steel in collums is also an interesting alternative to sttifen the structure. Beyond of some practical examples, others themes related to the design of this type of structure like: stability in the erection process, efforts in the end of the erection process, time-dependent effects, non symmetrical and plastic behavior of the connections, are also treated in this work.

鋼結構業的現行作業方式充滿了無效率的工作與浪費。本研究希望藉由豐田及時生產系統導入鋼結構製造，以消除生產過程中現存的各項浪費。本研究的目的主要是以個案公司為例，探討鋼結構業現行生產過程及豐田及時生產系統應用於鋼結構製造。 本研究首先介紹豐田及時生產系統架構，以了解豐田式的精神所在，再詳細介紹及時生產系統的概念及優點，並比較豐田即時生產系統和鋼結構現行生產方式。 其次藉由相關文獻的回顧，探討豐田及時生產系統於製造業應用方式與其效益。由於鋼結構業與一般製造業無論在形態上或是作業方式上，都有顯著的差異存在，因此於製造規劃階段，除說明導入豐田及時生產系統所需具備的合理化作業外，擬配合漸進式吊裝規劃技術以消除兩者間之差異。 經由親自到生產現場去查訪並與作業主管做雙向溝通，瞭解個案公司在現行生產管理上存在的問題。利用橋樑及H組合型鋼兩條試驗生產線進行檢討，評估豐田及時生產系統觀念應用於鋼結構製造過程之中可能產生的問題與解決對策，以擬定豐田及時生產系統導入鋼結構之方式與注意事項，並評估其效益。 / There has been full of inefficiency and waste in current operation method of steel structure industries. This study hopes to utilize Toyota Just-in-time Production System for fabrication of steel structure to eliminate current each waste during the production process. The main purpose of this study is take case company as an example to discuss the following respects: 1.The current production process of steel structure industries. 2.The application of Toyota Just-in-time Production System in the steel structure fabrication. This study first introduces the framework of Toyota Just-in-time Production System to apprehend its spirit and then explains the concept and advantage of Toyota Just-in-time Production System explicitly. Also, this study compares Toyota Just-in-time Production System with current production method of steel structure industries. Second, according to the glancing of related document reference, this study discusses the application method and its profit for Toyota Just-in-time Production System in the manufacturing industries. As there are significant differences in structure or operation method between steel structure industries and manufacturing industries, thus this study utilizes gradual erection plan to match Toyota Just-in-time Production System during the period of fabrication process plan to eliminate the distinction between these two industries besides explaining the necessary rationalized operation when leading Toyota Just-in-time Production System in. Having visited the production line and made mutual communication with operation chief, this study has found the current problems existing in production management in case company. According to this discovery, this study uses bridge production line and built-up H section production line as pilot implementation to discuss the possible question and solution policy for Toyota Just-in-time Production System concept applied in steel structure fabrication so as to frame the procedures and criterion of Toyota Just-in-time Production System in fabrication of steel structure and also to evaluate its profit. / 目 錄 誌謝詞……………………………………………………………………一 中文摘要…………………………………………………………………二 ABSTRACT………………………………………………………………三 目錄………………………………………………………………………四 圖次………………………………………………………………………六 表次………………………………………………………………………八 第一章 緒論…………………………………………………………………1 第一節 研究動機與目的 …………………………………………1 第二節 研究步驟與範圍…………………………………………4 第三節 研究架構 …………………………………………………6 第二章 豐田及時生產系統之文獻探討………………………………….7 第一節 及時化（Just In Time）的基本觀念………………………7 第二節 豐田及時生產系統的主要目的……………………….12 第三節 豐田及時生產系統的執行方法……………………….14 第四節 豐田及時生產系統的構成因素……………………….31 第五節 實施豐田及時生產系統的優點及原則………………32 第三章 鋼結構業引進豐田及時生產系統之可行性分析……………36 第一節 鋼結構業生產方式概要………………………………36 第二節 鋼結構業生產方式與豐田及時生產系統比較………44 第四章 中國鋼鐵結構公司導入豐田及時生產系統個案……………58 第一節 生產製造現況與問題分析……………………………58 第二節 豐田及時生產系統導入及效益評估 ………………73 第三節 導入限制條件之評估 ……………………………109 第五章 結論與建議……………………………………………………110 第一節 結論……………………………………………………110 第二節 建議 …………………………………………………112 參考文獻…………………………………………………………….113 附錄-個案公司訪談說明……………………….……………………118 作者簡歷……………………………………………………………………123 表 次 表1-1 台灣市場鋼結構用量統計表……………………………………2 表2-1 自�C化與自動化的比較…………………………………………27 表4-1 鋼橋漸進式吊裝規劃表範例……………………………………80 表4-2 鋼橋單一循環每日作業規劃表範例…………………………80 表4-3 中國鋼鐵結構公司91年鋼架類別統計表…………………….82 表4-4 中國鋼鐵結構公司91年工程類別產量表……………………83 表4-5 鋼橋月生產計劃表範例…………………………………………91 表4-6 鋼橋日生產計劃表範例…………………………………………92 表4-7 鋼橋批量生產表範例…………………………………………..94 表4-8鋼橋鑽孔績效比較表…………………………………………..…97 表4-9鋼橋本體板組合績效比較表…………………………………….97 表4-10鋼橋本體板電銲績效比較表……………………………………97 表4-11鋼橋學習效益………………..………………………………….100 表4-12鋼橋作業面積使用比較表…………………………………….102 表4-13鋼橋庫存量績效比較表….……………………………………102 表4-14 H組合型鋼腹板加工分類表…………………………………106 表4-15切割火口與鋼材板厚對照表..………………………………… 106 表4-16電銲條件表………………………………………………………107 表4-17 切割作業面積及在製品庫存量比較表……………………108 表4-18 H組合型鋼品質標準比較表…………………………………108 圖次 圖1-1 研究架構圖…………………………………………………………6 圖2-1拉式生產系統流程圖……………………………………………11 圖2-2豐田及時生產系統架構圖………………………………………13 圖2-3及時化架構圖……………………………………………………16 圖2-4工作標準化要素圖………………………………………………19 圖2-5看板方式流程圖…………………………………………………24 圖2-6自�C化架構圖……………………………………………………28 圖3-1鋼架製造流程圖…………………………………………………39 圖3-2鋼橋製造流程圖…………………………………………………40 圖3-3鋼架吊裝流程圖…………………………………………………41 圖3-4鋼橋吊裝流程圖…………………………………………………42 圖4-1小板件加工流程圖………………………………………………59 圖4-2 H型鋼組立程序圖…………………………………………………60 圖4-3方型鋼組立程序圖………………………………………………61 圖4-4生產計劃流程圖…………………………………………………66 圖4-5鋼柱加工流程圖…………………………………………………67 圖4-6鋼樑加工流程圖…………………………………………………68 圖4-7橋樑加工流程圖…………………………………………………69 圖4-8鋼橋構段分區圖範例……………………………………………81 圖4-9中國鋼鐵結構公司91年鋼架類別統計圖……………………82 圖4-10中國鋼鐵結構公司91年工程類別圖…………………………83 圖4-11鋼橋單一作業循環甘特圖範例……………………………….84 圖4-12橋樑構段圖………………………………………………………85 圖4-13 橋樑生產流程圖………………………………………………86 圖4-14 改善後橋樑生產流程圖.………………………………………89 圖4-15 鋼橋主生產時程圖範例.………………………………………90 圖4-16 鋼橋工作圖範例………………………………………………93 圖4-17 H組合型鋼圖……………………………………………………103 圖4-18H組合型鋼生產流程圖…………………………………………105