Introdução: A avaliação do consumo alimentar de crianças e adolescentes representa um desafio na pesquisa epidemiológica. O Recordatório de 24 horas (R24h) é considerado um ótimo método para coletar dados dietéticos e é muito utilizado no acompanhamento alimentar da população. Porém requer entrevistadores altamente treinados, o que dificulta o uso em pesquisas de grandes amostras. Objetivo. Desenvolver um Recordatório de 24 horas estruturado e computadorizado (R24ec) para a avaliação do consumo alimentar de escolares e testar a usabilidade deste sistema. Métodos. Os alimentos escolhidos para o R24ec foram pré-definidos a partir de um banco de dados secundário. O R24ec inclui seis refeições prédefinidas, embutidas em questões que contextualizam as rotinas e locais do dia anterior. Contém ainda uma lista de alimentos dividida em grupos alimentares e subgrupos. Durante a entrevista, itens não listados podem ser adicionados em espaço previsto para este uso. A base de dados de composição de alimentos é composta pela tabela TACO. O R24ec ainda inclui um atlas fotográfico, contendo diferentes tipos de alimentos que serve como auxílio visual para que os entrevistados possam selecionar os tamanhos de porções. Realizou-se um teste com o R24ec em uma escola pública de Guarulhos com 73 escolares. A usabilidade foi testada por 17 profissionais da área da tecnologia da informação através de um questionário que avalia a usabilidade de sistemas utilizando a lógica Fuzzy baseado na ISO. Resultados. Foi desenvolvido um instrumento computadorizado contendo uma lista de aproximadamente 120 alimentos com média de três tamanhos de porções. Dos escolares pesquisados, cerca de 45 por cento fizeram alguma observação referente ao sistema durante o preenchimento do R24ec. 8,2 por cento tiveram problemas em relação à manipulação do sistema e 30 por cento tiveram problemas no preenchimento devido à incompreensão das perguntas, devido à idade ou maturidade. 67 por cento não cometeram erros ao preencher o R24ec. O teste de usabilidade apresentou para as métricas Controle de Erros, Eficiência e Satisfação amplitude Média (2), representando maior grau de confiança no resultado obtido. As métricas Facilidade de Aprender, Facilidade de Relembrar e Eficácia apresentaram amplitude Alta (3), o que representa um grau de confiança baixo. Conclusão. O Recordatório de 24 horas estruturado foi desenvolvido com sucesso e será hospedado no sitio da Universidade de São Paulo. O teste na escola com estudantes de 5ª a 8ª série apontou uma boa interação entre usuários e o sistema. O estudo de usabilidade com profissionais teve boas avaliações, porém deveria ser refeito com uma população maior para obterse maior confiança nos resultados / Introduction: The assessment of children and teenagers food intake brings a new challenge in epidemiological research. The 24-hour Dietary Recall (R24h) is considered an efficient method to collect diet data and is also very much used in population food monitoring. However, it requires highly qualified interviewers and this fact therefore generates great difficulty to the use of great sample research. Objectives: It aims at developing a structured and automated 24-hour Dietary Recall (R24ec) to evaluate school children food intake and test the usability of this system. Methods: The foods chosen to R24ec were pre-defined from a secondary database. The R24ec includes six pre-defined meals, incorporated in questions that contextualize the routines and places of the day before. It also contains a list of food divided in food groups and subgroups. During the interview, non-listed items can be included in proper blanks for this purpose. The database of food combination is composed of the TACO chart. The R24ec also includes a food photographic atlas, containing different types of food, used as visual help so that the interviewees can select the portion sizes. A test with school children was carried out with R24ec in a public school of Guarulhos. The usability was tested by 17 Information Technology professionals through a questionnaire that assesses the usability of systems using the Fuzzy logic, based on ISO. Results: An automated tool was developed, containing a list of approximately 120 types of food with an average of three portion sizes. Among the school children evaluated, around 45 per cent made some observation referring to the system during the R24ec filling process. 8,2 per cent had problems to manipulate the system and 30 per cent had problems to fill out the R24ec due to question misunderstandings, fact that can be related to the low level of maturity of the candidates. 67 per cent did not make any mistakes filling out the R24ec. The usability test presented to the metric Error Control, Efficiency and Satisfaction an average amplitude (2), representing a higher level of reliability in the obtained results. The metrics Facility to Learn, Facility to Remember and Efficiency presented high amplitude (3), which represents a low level of reliability. Conclusion: The structured 24-hour Dietary Recall was successfully developed and it will be posted in the Universidade de São Paulo website. The test with students from the Elementary School revealed good interaction between users and system. The usability study with professionals obtained good reviews, but it should be reapplied to a bigger audience in order to obtain a higher level of reliability towards the results

abstract: Urinary sucrose and fructose has been suggested as a predictive biomarker of total sugars intake based on research involving UK adults. The purpose of this study was to determine the association between total sugars consumption and 24-hour urinary sucrose and fructose (24uSF) in US adult population and to investigate the effect of physical activity on this association. Fifty seven free-living healthy subjects 20 to 68 years old, participated in a 15-day highly controlled feeding study, consuming their habitual diet, provided by the research metabolic kitchen. Dietary sugars were estimated using Nutrition Data System for Research (NDSR). Subjects collected eight 24-hour urine samples measured for urinary sucrose and fructose. Physical activity was assessed daily using a validated 15-day log that inquired about 38 physical activities across six domains; home activities, transportation, occupation, conditioning, sports and leisure. The mean total sugars intake and added sugars intake of the sample was 112.2 (33.1) g/day and 65.8 (29.0) g/day (9.7%EI), respectively. Significant moderate positive correlation was found between 15-d mean total sugars intake and 8-day mean 24uSF (r = 0.56, p < 0.001). Similarly, added sugars were moderately correlated with 24uSF (r = 0.56, p < 0.001), while no correlation was found between naturally-occurring sugars and 24uSF (r = 0.070, p < 0.001). In a linear multiple regression, total and added sugars each explained 30% of variability in 24uSF (Adjusted R2, p value; total sugars: 0.297, 0.001; added sugars: 0.301, p < 0.001). Physical activity had no effect on the association between dietary and urinary sugars in neither the correlation nor the linear regression analysis. 24uSF can be used as a biomarker for total and added sugars consumption in US adults, although its predictability was weaker compared to findings involving UK adults. No evidence was found showing that physical activity levels affect the association between 24uSF and total sugars intake in US adults. More detailed investigation through future feeding studies including subjects with wide range of sugars intake and of different ethnic/racial backgrounds are needed to better understand the characteristics of the biomarker and its uses. / Dissertation/Thesis / Masters Thesis Nutrition 2019

Darbo tikslas: Įvertinti Kaišiadorių rajono gyventojų mitybą ir jos ryšį su arterine hipertenzija. Uždaviniai: Nustatyti Kaišiadorių rajono 25-64 m. gyventojų paros maisto davinio sudėtį. Įvertinti Kaišiadorių rajono 25-64 m. gyventojų mitybą ir jos sąsajas su socialiniais veiksniais. Nustatyti Kaišiadorių gyventojų mitybos įpročių pokyčius nuo1999 m. iki 2007 m. Įvertinti arterinės hipertenzijos paplitimą tarp Kaišiadorių gyventojų bei jos kontrolės būklę 1999 m. ir 2007 m. Įvertinti gyventojų mitybos ryšį su arterine hipertenzija. Tyrimo metodika. Naudojant 24 val. mitybos apklausos metodą, 1999 m. buvo apklausti 326 atsitiktinai atrinkti 25-64 m. Kaišiadorių rajono gyventojai. Suskaičiuota paros maisto davinio energinė vertė ir maisto medžiagų kiekiai. 1999 m. ir 2007 m. (n=257) buvo atlikta dažnuminė mitybos apklausa. Sveikatos patikrinimų metu matuotas arterinis kraujospūdis. Statistinė duomenų analizė atlikta naudojant SPSS programos 12 versiją. Skirtumai tarp skirstinių vertinti, taikant Mann-Whitney ir Kruskal-Wallis testus. Vertinant hipertenzijos paplitimo skirtumus tarp grupių, taikytas Z ir χ2 kriterijus. Statistiškai reikšmingu laikytinas rezultatas, kai paklaidos tikimybė p<0,05. Rezultatai. Kaišiadorių rajono 25-64 m. gyventojai vartojo pakankamai baltymų, bet per daug riebalų ir per mažai angliavandenių. Vyrų ir moterų maisto davinyje buvo per didelis cholesterolio ir natrio kiekis bei nepakankamas maistinių skaidulų ir kalcio kiekis. Vyrai su maistu gavo... [toliau žr. visą tekstą] / Aim of the study: to evaluate nutrition habits of Kaišiadoris region population and to examine the association between diet and hypertension. Objectives: to examine daily energy and nutrient intake of population aged 25-64 in Kaišiadoris region in relation to social factors; to evaluate changes in nutrition habits between 1999 and 2007; to assess the prevalence of hypertension and control status; to examine the association between diet and hypertension. Methods: 24 hour dietary recall and food frequency questionnaire were used to examine nutrition of randomly selected people in Kaišiadoris region: 326 persons aged 25-64 years were examined in 1999 and 257 - in 2007. The daily energy and nutrient intake was calculated. Blood pressure was measured in both surveys. Statistical analyses were performed using statistical software package “SPSS 12”. The differences in the distributions of energy and nutrients between various groups were assessed by Mann-Whitney and Kruskal-Wallis tests. The differences in prevalence of hypertension were assessed by Z test and χ2 criterion. The difference was considered to be statistically significant when p<0.05. Results. The average total fat intake of population of Kaišiadoris region was above recommendations for healthy nutrition and the average intake of carbohydrates was insufficient. The intake of protein was sufficient. The diet of men and women contained too much cholesterol and sodium and lacked fibers and calcium. Daily energy and nutrient... [to full text]

Estudo de caso-controle conduzido na cidade de Porto Alegre, com o objetivo de avaliar a associação do consumo de gorduras saturadas com sobrepeso e obesidade. Foram incluídas 52 crianças e adolescentes com idades entre seis e 19 anos, sendo 26 eutróficos e 26 com sobrepeso ou obesidade. Todos responderam a um questionário que contemplava história familiar, atividade física e hábitos alimentares. O consumo diário de alimentos ricos em ácidos graxos saturados no grupo de casos e controles foi, em média, de 31,63 mg e 23,18 mg, respectivamente (p<0,05). Quando a ingestão foi avaliada pelo questionário de freqüência alimentar, os casos apresentaram consumo mais freqüente de produtos lácteos e carne de gado. Este estudo demonstrou que fatores relacionados a história familiar de obesidade, hábitos alimentares inadequados e sedentarismo influenciam no desenvolvimento da obesidade desde a infância, e que a intervenção nutricional precoce torna-se necessária para prevenção de fatores de risco associados ao desenvolvimento desta patologia. / A case-control study was conducted in Porto Alegre, south Brazil, with the aim of evaluating the association of consumption of saturated fat with overweight or obesity. It was included 52 children and adolescents aged between 6 and 19 years old, being 26 eutrophic people and 26 overweight or obese people. All of them answered a questionnaire that contemplated family history, physical activity and feeding habits. The daily consumption of food rich in saturated fatty acid in the group of cases and controls were in average 31,63 mg and 23,18 mg, respectively (p< 0.05). There was a variation according to the type of food when ingestion was evaluated through the questionnaire of feeding frequency. This study showed that the factors related to family history of obesity, inadequate feeding habits and sedentariness influenced upon the development of obesity since childhood, and that precocious nutritional intervention is necessary to the prevention of risk factors associated to the development of this pathology.

Estudo de caso-controle conduzido na cidade de Porto Alegre, com o objetivo de avaliar a associação do consumo de gorduras saturadas com sobrepeso e obesidade. Foram incluídas 52 crianças e adolescentes com idades entre seis e 19 anos, sendo 26 eutróficos e 26 com sobrepeso ou obesidade. Todos responderam a um questionário que contemplava história familiar, atividade física e hábitos alimentares. O consumo diário de alimentos ricos em ácidos graxos saturados no grupo de casos e controles foi, em média, de 31,63 mg e 23,18 mg, respectivamente (p<0,05). Quando a ingestão foi avaliada pelo questionário de freqüência alimentar, os casos apresentaram consumo mais freqüente de produtos lácteos e carne de gado. Este estudo demonstrou que fatores relacionados a história familiar de obesidade, hábitos alimentares inadequados e sedentarismo influenciam no desenvolvimento da obesidade desde a infância, e que a intervenção nutricional precoce torna-se necessária para prevenção de fatores de risco associados ao desenvolvimento desta patologia. / A case-control study was conducted in Porto Alegre, south Brazil, with the aim of evaluating the association of consumption of saturated fat with overweight or obesity. It was included 52 children and adolescents aged between 6 and 19 years old, being 26 eutrophic people and 26 overweight or obese people. All of them answered a questionnaire that contemplated family history, physical activity and feeding habits. The daily consumption of food rich in saturated fatty acid in the group of cases and controls were in average 31,63 mg and 23,18 mg, respectively (p< 0.05). There was a variation according to the type of food when ingestion was evaluated through the questionnaire of feeding frequency. This study showed that the factors related to family history of obesity, inadequate feeding habits and sedentariness influenced upon the development of obesity since childhood, and that precocious nutritional intervention is necessary to the prevention of risk factors associated to the development of this pathology.

abstract: Background: Healthy eating plays critical roles in the prevention of many chronic diseases, but there are many barriers in life that prevent people from adopting and maintaining healthy diets. Thus, identifications of barriers that people perceive they have in trying to eat healthy can guide the strategies for dietary behavior change interventions by taking account of the barriers. Objective: The purpose of this study was to identify and quantify the perceived barriers to healthy eating (PBHE), to investigate the relationship between socioeconomic factors and PBHE, and to explore the associations between PBHE and dietary intake among parents of elementary-school aged children living in South Phoenix, AZ. Methods: Socioeconomic factors and PBHEs were obtained via survey and diet was assessed by two interviewer-assisted 24 h diet recalls. The associations between employment and PBHEs, education and PBHEs, and household monthly income and PBHEs were analyzed by Mann-Whitney Test, Kruskal Wallis Test, and Spearman’s correlation test, respectively. The relationship between PBHEs and dietary intake were analyzed by Spearman’s correlation test. Linear regression was used to assess the associations between total PBHE, and dietary intake (including added sugar, fruit and vegetable), adjusted by covariates (including socioeconomic status, birth country, age and gender). Results: Of 149 participants who completed the survey (mean age = 38.47±7.08 y), 136 completed the 24 h diet recalls. The mean reported total, social support, emotions and daily mechanics PBHE scores were 2.63±0.91, 2.52±1.16, 2.71±1.06, and 2.58±0.95, respectively, out of a 5-point scale. Daily fruit, vegetable, sugar-sweetened beverage, sweetened foods, and added sugar intake were reported as 1.66±1.56 servings, 2.45±1.43 servings, 1.19±1.30 servings, 2.02±2.12 servings and 49.93±31.17 g, respectively. Employment status was significantly associated with total PBHE (Z = -2.28, p=0.023), and support PBHE (Z = -2.623, p=0.009). Education was significantly related to total PBHE (χ2 = -7.987, p=0.046), and daily mechanics PBHE (χ2= 11.735, p=0.008). Household monthly income levels were significantly correlated to daily mechanics PBHE (r = -0.265, p=0.005). Added sugar was positively correlated with total PBHE (r=0.202, p=0.020), emotions PBHE (r=0.239, p=0.006), and daily mechanics PBHE (r=0.179, p=0.040). Sugar sweetened beverage intake was significantly related to emotions PBHE (r=0.183, p=0.035). When adjusting for socioeconomic factors in the regression analysis, there was no significant association between PBHE and diet intake. Conclusion: Overall, results suggest PBHEs listed in this study are mainly associated with socioeconomic factors, but they are not related to diet intake. Future studies will focus on the precise role of overcoming some identified barriers in improving healthy eating behaviors, and the causality between barriers and healthy eating. / Dissertation/Thesis / Masters Thesis Nutrition 2018

Estudo de caso-controle conduzido na cidade de Porto Alegre, com o objetivo de avaliar a associação do consumo de gorduras saturadas com sobrepeso e obesidade. Foram incluídas 52 crianças e adolescentes com idades entre seis e 19 anos, sendo 26 eutróficos e 26 com sobrepeso ou obesidade. Todos responderam a um questionário que contemplava história familiar, atividade física e hábitos alimentares. O consumo diário de alimentos ricos em ácidos graxos saturados no grupo de casos e controles foi, em média, de 31,63 mg e 23,18 mg, respectivamente (p<0,05). Quando a ingestão foi avaliada pelo questionário de freqüência alimentar, os casos apresentaram consumo mais freqüente de produtos lácteos e carne de gado. Este estudo demonstrou que fatores relacionados a história familiar de obesidade, hábitos alimentares inadequados e sedentarismo influenciam no desenvolvimento da obesidade desde a infância, e que a intervenção nutricional precoce torna-se necessária para prevenção de fatores de risco associados ao desenvolvimento desta patologia. / A case-control study was conducted in Porto Alegre, south Brazil, with the aim of evaluating the association of consumption of saturated fat with overweight or obesity. It was included 52 children and adolescents aged between 6 and 19 years old, being 26 eutrophic people and 26 overweight or obese people. All of them answered a questionnaire that contemplated family history, physical activity and feeding habits. The daily consumption of food rich in saturated fatty acid in the group of cases and controls were in average 31,63 mg and 23,18 mg, respectively (p< 0.05). There was a variation according to the type of food when ingestion was evaluated through the questionnaire of feeding frequency. This study showed that the factors related to family history of obesity, inadequate feeding habits and sedentariness influenced upon the development of obesity since childhood, and that precocious nutritional intervention is necessary to the prevention of risk factors associated to the development of this pathology.

Introdução: A avaliação do consumo alimentar de crianças e adolescentes representa um desafio na pesquisa epidemiológica. O Recordatório de 24 horas (R24h) é considerado um ótimo método para coletar dados dietéticos e é muito utilizado no acompanhamento alimentar da população. Porém requer entrevistadores altamente treinados, o que dificulta o uso em pesquisas de grandes amostras. Objetivo. Desenvolver um Recordatório de 24 horas estruturado e computadorizado (R24ec) para a avaliação do consumo alimentar de escolares e testar a usabilidade deste sistema. Métodos. Os alimentos escolhidos para o R24ec foram pré-definidos a partir de um banco de dados secundário. O R24ec inclui seis refeições prédefinidas, embutidas em questões que contextualizam as rotinas e locais do dia anterior. Contém ainda uma lista de alimentos dividida em grupos alimentares e subgrupos. Durante a entrevista, itens não listados podem ser adicionados em espaço previsto para este uso. A base de dados de composição de alimentos é composta pela tabela TACO. O R24ec ainda inclui um atlas fotográfico, contendo diferentes tipos de alimentos que serve como auxílio visual para que os entrevistados possam selecionar os tamanhos de porções. Realizou-se um teste com o R24ec em uma escola pública de Guarulhos com 73 escolares. A usabilidade foi testada por 17 profissionais da área da tecnologia da informação através de um questionário que avalia a usabilidade de sistemas utilizando a lógica Fuzzy baseado na ISO. Resultados. Foi desenvolvido um instrumento computadorizado contendo uma lista de aproximadamente 120 alimentos com média de três tamanhos de porções. Dos escolares pesquisados, cerca de 45 por cento fizeram alguma observação referente ao sistema durante o preenchimento do R24ec. 8,2 por cento tiveram problemas em relação à manipulação do sistema e 30 por cento tiveram problemas no preenchimento devido à incompreensão das perguntas, devido à idade ou maturidade. 67 por cento não cometeram erros ao preencher o R24ec. O teste de usabilidade apresentou para as métricas Controle de Erros, Eficiência e Satisfação amplitude Média (2), representando maior grau de confiança no resultado obtido. As métricas Facilidade de Aprender, Facilidade de Relembrar e Eficácia apresentaram amplitude Alta (3), o que representa um grau de confiança baixo. Conclusão. O Recordatório de 24 horas estruturado foi desenvolvido com sucesso e será hospedado no sitio da Universidade de São Paulo. O teste na escola com estudantes de 5ª a 8ª série apontou uma boa interação entre usuários e o sistema. O estudo de usabilidade com profissionais teve boas avaliações, porém deveria ser refeito com uma população maior para obterse maior confiança nos resultados / Introduction: The assessment of children and teenagers food intake brings a new challenge in epidemiological research. The 24-hour Dietary Recall (R24h) is considered an efficient method to collect diet data and is also very much used in population food monitoring. However, it requires highly qualified interviewers and this fact therefore generates great difficulty to the use of great sample research. Objectives: It aims at developing a structured and automated 24-hour Dietary Recall (R24ec) to evaluate school children food intake and test the usability of this system. Methods: The foods chosen to R24ec were pre-defined from a secondary database. The R24ec includes six pre-defined meals, incorporated in questions that contextualize the routines and places of the day before. It also contains a list of food divided in food groups and subgroups. During the interview, non-listed items can be included in proper blanks for this purpose. The database of food combination is composed of the TACO chart. The R24ec also includes a food photographic atlas, containing different types of food, used as visual help so that the interviewees can select the portion sizes. A test with school children was carried out with R24ec in a public school of Guarulhos. The usability was tested by 17 Information Technology professionals through a questionnaire that assesses the usability of systems using the Fuzzy logic, based on ISO. Results: An automated tool was developed, containing a list of approximately 120 types of food with an average of three portion sizes. Among the school children evaluated, around 45 per cent made some observation referring to the system during the R24ec filling process. 8,2 per cent had problems to manipulate the system and 30 per cent had problems to fill out the R24ec due to question misunderstandings, fact that can be related to the low level of maturity of the candidates. 67 per cent did not make any mistakes filling out the R24ec. The usability test presented to the metric Error Control, Efficiency and Satisfaction an average amplitude (2), representing a higher level of reliability in the obtained results. The metrics Facility to Learn, Facility to Remember and Efficiency presented high amplitude (3), which represents a low level of reliability. Conclusion: The structured 24-hour Dietary Recall was successfully developed and it will be posted in the Universidade de São Paulo website. The test with students from the Elementary School revealed good interaction between users and system. The usability study with professionals obtained good reviews, but it should be reapplied to a bigger audience in order to obtain a higher level of reliability towards the results

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