Made available in DSpace on 2014-12-17T14:27:16Z (GMT). No. of bitstreams: 1 NiloFPJ_DISSERT.pdf: 893945 bytes, checksum: d28461f66c3f9df0b3e1690450e63046 (MD5) Previous issue date: 2011-11-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The objective of this work was about fixing the free will paradigm as negative evaluation of political rights which presents a new classification dde such rights, producing species: a) conditions of eligibility autonomous (free will), b) eligibility requirements heteronomous (will third party) and c) ineligibility (court decisions / administrative). This morality and life history as a condition of eligibility unattended, making a hermeneutic analysis of art. 14, ? 9 of the Constitution, considering the justification of the views of the voting Minister Carlos Ayres Brito Appeal in Ordinary No 1069/2006 of the Supreme Electoral Tribunal (where Eurico Miranda). Are fixed concepts of morality and life history from the perspective of the moral act freely and consciously. Has resulted in the identification of the moral virtues of honesty and integrity, which are voluntary acts as a reference to morality and integrity respectively. Justifies the morality and life history as a condition of eligibility unattended. who depend exclusively on the willingness of the candidate. It is noteworthy that the conditions for eligibility as a factual finding does not violate the law and does not allow punitive sanctions or setting a deadline in case of refusal to register the application. Attributed to political parties to take responsibility in their statutes moral criteria for the nomination convention in pre candidates, giving an ethical dimension. Analyzes the law under the Clean Record of morality and life history of the candidate and the possible impact on the electoral context. / O objetivo deste trabalho ? versar sobre a fixa??o do livre arb?trio como paradigma avaliativo dos direitos pol?ticos de recep??o apresentando uma nova classifica??o de tais direitos, produzindo as esp?cies: a) condi??es de elegibilidade aut?nomas (vontade livre); b) condi??es de elegibilidade heter?nomas (vontade de terceiros) e c) inelegibilidades (decis?es judiciais/administrativas). Trata ainda da moralidade e da vida pregressa como condi??o aut?noma de elegibilidade, fazendo uma an?lise hermen?utica do art. 14, ? 9? da Constitui??o Federal, considerando a fundamenta??o dos voto de vistas do Ministro Carlos Ayres Brito, no Recurso Ordin?rio n? 1069/2006 do Tribunal Superior Eleitoral (caso Eurico Miranda). Nele s?o fixados os conceitos de moralidade e da vida pregressa dentro da perspectiva do ato moral livre e consciente. Tem como resultado a identifica??o das virtudes morais da honradez e da honestidade, sendo estas atos volunt?rios, como refer?ncia ? moralidade e ? probidade respectivamente. Justifica-se a moralidade e a vida pregressa como condi??o aut?noma de elegibilidade as quais dependem exclusivamente da vontade do candidato. Ressalta-se as condi??es de elegibilidade como uma constata??o f?tica que n?o viola o direito e n?o admite san??es punitivas ou fixa??o de prazo, em caso de indeferimento do registro da candidatura. Atribui aos partidos pol?ticos a responsabilidade em adotar em seus estatutos crit?rios morais para indica??o em conven??o de pr? candidatos, dando uma dimens?o ?tica. Analisa a Lei da Ficha Limpa sob a moralidade e vida pregressa do candidato e as poss?veis incid?ncias no contexto eleitoral.

Made available in DSpace on 2015-02-24T17:54:40Z (GMT). No. of bitstreams: 1 NiloFPJ_DISSERT_PARCIAL.pdf: 334406 bytes, checksum: d62c2d974e7774921b2113f8a9223135 (MD5) Previous issue date: 2010-11-30 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The objective of this work was about fixing the free will paradigm as negative evaluation of political rights which presents a new classification dde such rights, producing species: a) conditions of eligibility autonomous (free will), b) eligibility requirements heteronomous (will third party) and c) ineligibility (court decisions / administrative). This morality and life history as a condition of eligibility unattended, making a hermeneutic analysis of art. 14, ? 9 of the Constitution, considering the justification of the views of the voting Minister Carlos Ayres Brito Appeal in Ordinary No 1069/2006 of the Supreme Electoral Tribunal (where Eurico Miranda). Are fixed concepts of morality and life history from the perspective of the moral act freely and consciously. Has resulted in the identification of the moral virtues of honesty and integrity, which are voluntary acts as a reference to morality and integrity respectively. Justifies the morality and life history as a condition of eligibility unattended. who depend exclusively on the willingness of the candidate. It is noteworthy that the conditions for eligibility as a factual finding does not violate the law and does not allow punitive sanctions or setting a deadline in case of refusal to register the application. Attributed to political parties to take responsibility in their statutes moral criteria for the nomination convention in pre candidates, giving an ethical dimension. Analyzes the law under the Clean Record of morality and life history of the candidate and the possible impact on the electoral context / O objetivo deste trabalho ? versar sobre a fixa??o do livre arb?trio como paradigma avaliativo dos direitos pol?ticos de recep??o apresentando uma nova classifica??o de tais direitos, produzindo as esp?cies: a) condi??es de elegibilidade aut?nomas (vontade livre); b) condi??es de elegibilidade heter?nomas (vontade de terceiros) e c) inelegibilidades (decis?es judiciais/administrativas). Trata ainda da moralidade e da vida pregressa como condi??o aut?noma de elegibilidade, fazendo uma an?lise hermen?utica do art. 14, ? 9? da Constitui??o Federal, considerando a fundamenta??o dos voto de vistas do Ministro Carlos Ayres Brito, no Recurso Ordin?rio n? 1069/2006 do Tribunal Superior Eleitoral (caso Eurico Miranda). Nele s?o fixados os conceitos de moralidade e da vida pregressa dentro da perspectiva do ato moral livre e consciente. Tem como resultado a identifica??o das virtudes morais da honradez e da honestidade, sendo estas atos volunt?rios, como refer?ncia ? moralidade e ? probidade respectivamente. Justifica-se a moralidade e a vida pregressa como condi??o aut?noma de elegibilidade as quais dependem exclusivamente da vontade do candidato. Ressalta-se as condi??es de elegibilidade como uma constata??o f?tica que n?o viola o direito e n?o admite san??es punitivas ou fixa??o de prazo, em caso de indeferimento do registro da candidatura. Atribui aos partidos pol?ticos a responsabilidade em adotar em seus estatutos crit?rios morais para indica??o em conven??o de pr? candidatos, dando uma dimens?o ?tica. Analisa a Lei da Ficha Limpa sob a moralidade e vida pregressa do candidato e as poss?veis incid?ncias no contexto eleitoral

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-07-04T14:27:11Z No. of bitstreams: 1 HeloiseAndriellyNascimentoCosta_DISSERT.pdf: 1809358 bytes, checksum: a0116fb35021db655e6b0670cf1dfe83 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-07-14T12:44:57Z (GMT) No. of bitstreams: 1 HeloiseAndriellyNascimentoCosta_DISSERT.pdf: 1809358 bytes, checksum: a0116fb35021db655e6b0670cf1dfe83 (MD5) / Made available in DSpace on 2017-07-14T12:44:57Z (GMT). No. of bitstreams: 1 HeloiseAndriellyNascimentoCosta_DISSERT.pdf: 1809358 bytes, checksum: a0116fb35021db655e6b0670cf1dfe83 (MD5) Previous issue date: 2017-02-20 / A delimita??o das Florestas Estacionais deve ser realizada incluindo v?rios aspectos, como solo, relevo e vegeta??o. No Nordeste brasileiro, a Floresta Estacional Decidual ocorre em zona de contato entre a Caatinga, o Cerrado e a Mata Atl?ntica, sendo faixas estreitas e transicionais. No RN ocorre uma versatilidade florestal entre esses ecossistemas. A varia??o de altitude ? um fator importante de mudan?as de padr?es vegetacionais, no entanto pouco estudada em florestas nacionais e locais. O estudo foi realizado em uma ?rea de floresta decidual no munic?pio de Maca?ba. Esta foi dividida em 4 cotas, com diferen?a de 19 m de altitude entre elas. Foram coletados dados flor?sticos e fitossociol?gicos. Foi inventariado um total de 449 indiv?duos distribu?dos em 69 esp?cies, 49 g?neros e 36 fam?lias, sendo a fam?lia mais representativa a Fabaceae com 11 esp?cies e 164 indiv?duos, seguidas por Myrtaceae (10 esp?cies e 98 indiv?duos) e Malpighiaceae (4 esp?cies e 10 indiv?duos). As esp?cies Chamaecrista ensiformis (Vell.) H.S. Irwin & Barneby, Guapira laxa (Netto) Furlan e Zanthoxylum syncarpum Tul. obtiveram os maiores ?ndices Fr: 50, 45 e 50 e VI: 25,11%; 17,09% e 19,58% respectivamente. Nenhuma esp?cie ocorreu ao longo de todo gradiente, havendo uma entrada e sa?da de t?xons constante. Os valores da DCA foram de -0,851 e - 6,8794; os ?ndices de J?, 0,8432, (C) 0,9533 E H?3,532; C2 apresentou os melhores valores para todos os ?ndices analisados, evidenciando um excelente estagio de conserva??o e fitofisionomia relacionada ? Mata Atl?ntica; C1 e C4, apesar da dist?ncia, apresentam fitofisionomia da Caatinga e do Cerrado, enquanto C3 apresenta t?xons de ampla distribui??o entre os biomas nacionais. Assim, observa-se uma significante dissimilaridade na estrutura vegetacional de modo suave e cont?nuo. / The delimitation of Deciduous Forests should be done including several aspects, such as soil, relief and vegetation. In the Brazilian Northeast, the Decidual Seasonal forest occurs in a contact zone between the Caatinga, the Cerrado and the Atlantic Forest, being narrow and transitional bands. In the RN there is a forest versatility between these ecosystems. The altitude variation is an important factor of changes in vegetation patterns, however not deeply studied in national and local forests. The study was carried out in an area of deciduous forest in the county of Maca?ba, which was divided into 4 zones with a difference of 19 m of altitude between them, floristic and phytosociological data were collected. A total of 449 individuals distributed in 69 species, 49 genus and 36 families were inventoried. Fabaceae family was the most representative one with 11 species and 164 individuals, followed by Myrtaceae (10 species and 98 individuals) and Malpighiaceae (4 species and 10 indivisuals). Chamaecrista ensiformis (Vell.) H.S. Irwin & Barneby, Guapira laxa (Netto) Furlan e Zanthoxylum syncarpum Tul species obtained the higher indexes Fr: 50, 45 e 50 e VI: 25,11%; 17,09% e 19,58% , repectively. No species occurred along the entire gradient, with a constant input and output of taxa, DCA values was -0,851 e -6,8794, the indexes de of J?0,8432 , (C) 0,9533 E H?3,532; C2 presented the best values for all analyzed indexes evidencing an excellent stage of conservation and phytosanation related to the Atlantic Forest. Ci and C4 in spite of the distance they present Phytophysiognomy of Caatinga and Cerado; While C3 presents taxa of wide distribution among the national biomes. Thus a significant dissimilarity in the vegetative structure is observed in a smooth and continuous way.

Nesta dissertação de mestrado foram utilizadas duas ligas de alumínio (AA1100 e AA5052) para a produção de uma microestrutura final apresentando tamanho médio de grãos menores que um micrometro. Tal fato foi possível através do processo de Accumulated Roll Bonding (ARB). Tais materiais produzidos por ARB, foram caracterizados conforme propriedades mecânicas (microdureza, limite de escoamento e resistência) e microestruturais (tamanho de grão e macrotextura e composição química dos precipitados). O principal resultado para este trabalho foi a produção de tiras de AA 1100 e AA 5052 com tamanho médio de grão de 0,50 ± 0,04 m e 0,42 ± 0,06 m respectivamente. Tal resultado é coerente em comparação à literatura. Com relação aos resultados de textura, tais amostras apresentaram temperatura de recristalização acima de 473K e componente de textura típicas de cisalhamento (tanto para o material oriundo de fábrica como para o material laminado). Tal fato é esperado já que não se utilizou lubrificação durante ensaios de laminação. A propriedade mecânica de microdureza de AA 1100 ficou coerente com a literatura embora as propriedades de limite de escoamento e de resistência possuíram módulos abaixo da literatura. / The present work is related to the production, in a laboratory scale, of a metallic microstructure having a grain size smaller than one micrometer, through the Accumulated Roll Bonding technique(ARB), for two different aluminum alloys, namely AA 1100 and AA 5052. The strips obtained by the ARB technique have been characterized by their mechanical properties (microhardness, yield and tensile strength), and their microstructure (grain size, macrotexture and precipitates chemical composition). The main result of this work has been the rolling of AA1100 and AA5052 strips with an average grain size of 0.5 ± 0.04 m and 0.42 ± 0.06 m respectively. Such results are consistent with the literature. Relative to the texture, the samples presented a recrystallization temperature higher than 473K and typical shear texture components (due to the dry rolling conditions during the ARB). Microhardness of the AA110 was consistent with literature data; however yield and tensile strength presented values that were lower than those observed in the literature.

Made available in DSpace on 2015-04-15T12:50:29Z (GMT). No. of bitstreams: 1 457995.pdf: 837783 bytes, checksum: 7795cbaa95bbe4686962df85224abf3e (MD5) Previous issue date: 2014-03-25 / This dissertation intends to investigates the arguments that the Saint and Doctor of the Roman Catholic Church, Thomas Aquinas introduces in his thinking concerning of free will and the way that he envisages the Divine Providence. It analyzes the development of these issues over the course of patristic, as well as some of the main ideas of the thinkers who were the pillars of the catholic saint. With reference to his magnum opus, the Summa Theologica, this dissertation looks for the dimension of free will that humans have in front of the divine will in many issues of his work. / A presente disserta??o procura investigar os argumentos que o santo e doutor da Igreja Cat?lica Apost?lica Romana, Tom?s de Aquino, apresenta no seu pensamento referente ao livre-arb?trio e como este encontra espa?o perante a Divina Provid?ncia. Para tanto, analisa-se o desenvolvimento dessas quest?es ao decorrer da patr?stica, bem como as principais ideias de alguns dos pensadores que foram pilares do pensamento do santo cat?lico. Tendo como refer?ncia sua obra m?xima, a Suma Teol?gica, a disserta??o procura em diversas quest?es da obra a dimens?o do livre-arb?trio que o ser humano possui perante a vontade divina.

Nesta dissertação de mestrado foram utilizadas duas ligas de alumínio (AA1100 e AA5052) para a produção de uma microestrutura final apresentando tamanho médio de grãos menores que um micrometro. Tal fato foi possível através do processo de Accumulated Roll Bonding (ARB). Tais materiais produzidos por ARB, foram caracterizados conforme propriedades mecânicas (microdureza, limite de escoamento e resistência) e microestruturais (tamanho de grão e macrotextura e composição química dos precipitados). O principal resultado para este trabalho foi a produção de tiras de AA 1100 e AA 5052 com tamanho médio de grão de 0,50 ± 0,04 m e 0,42 ± 0,06 m respectivamente. Tal resultado é coerente em comparação à literatura. Com relação aos resultados de textura, tais amostras apresentaram temperatura de recristalização acima de 473K e componente de textura típicas de cisalhamento (tanto para o material oriundo de fábrica como para o material laminado). Tal fato é esperado já que não se utilizou lubrificação durante ensaios de laminação. A propriedade mecânica de microdureza de AA 1100 ficou coerente com a literatura embora as propriedades de limite de escoamento e de resistência possuíram módulos abaixo da literatura. / The present work is related to the production, in a laboratory scale, of a metallic microstructure having a grain size smaller than one micrometer, through the Accumulated Roll Bonding technique(ARB), for two different aluminum alloys, namely AA 1100 and AA 5052. The strips obtained by the ARB technique have been characterized by their mechanical properties (microhardness, yield and tensile strength), and their microstructure (grain size, macrotexture and precipitates chemical composition). The main result of this work has been the rolling of AA1100 and AA5052 strips with an average grain size of 0.5 ± 0.04 m and 0.42 ± 0.06 m respectively. Such results are consistent with the literature. Relative to the texture, the samples presented a recrystallization temperature higher than 473K and typical shear texture components (due to the dry rolling conditions during the ARB). Microhardness of the AA110 was consistent with literature data; however yield and tensile strength presented values that were lower than those observed in the literature.

Made available in DSpace on 2016-04-26T19:39:32Z (GMT). No. of bitstreams: 1 2009 - Deise Ferreira Xavier.pdf: 1914951 bytes, checksum: a3934201689ba192bf1c30664a804084 (MD5) Previous issue date: 2009-01-28 / A large proportion of the land in the Atlantic forest region in the south east of Brazil is today degraded with little utilisation for agriculture, grazing or forestry. The recuperation and development of these areas using traditional production systems suffers from two principal handicaps: much of the land is steep hillsides which hampers mechanised cultivation and facilitates soil erosion, and the low availability of plant nutrients, principally nitrogen. The integration of pastures with trees has become considered as a viable option to promote sustainable land use for animal production systems in this region. The objective of this project was to monitor the principal inputs, losses and transformations of N in a pasture of Brachiaria decumbens cv. Basilisk in monoculture (BM) and in comparison with the same grass in association with trees (SSP). The experiment was localised at the Experimental Station of Coronel Pacheco (Minas Gerais), with five animals per paddock under rotational grazing (six paddocks of 0.5 ha). The following parameters were monitored over a period of 12 months: forage availability, live weight gain and forage consumption of the animals, existing litter and the litter deposited in periods of 14 days. Considering the total litter deposited the consumed forage and the change in standing biomass over the 12 month period it was possible to estimate the net aerial primary productivity (NAPP) of the pasture and the cycling of nitrogen in the two pasture systems. The standing biomass showed an annual mean of 1,800 and 1,653 kg/ha in the silvopastoral (SSP) and grass-alone (BM) systems respectively. The live weight gains of the animals in SSP were higher with a mean of 0.51 kg/heifer/day. In the BM pasture the weight gain was 0.45 kg/heifer/day. The SSP promoted a significant increase in the deposition of litter. Calculations of the quantity of litter deposited in 14-day periods in the SSP and BM showed values of 21,812 and 16,264 kg DM/ha/year, respectively. The NAPP of the SSP pasture was greater with values of 26,202 and 279 kg/ha/year for DM and N, respectively. In the BM pasture the estimates of NAPP were 20,133 and 148 kg/ha/year for DM and N, respectively. The quantity of N exported from the pasture in the live weight gain was 8 kg/ha for the SSP and 7 kg/ha for the BM, corresponding to 16 and 18% of total consumed N for the SSP and BM pastures, respectively. The losses of N from faeces and urine also did not differ between the pasture systems. Owing to the presence of N2-fixing species of legume trees present in the SSP, and the beneficial effects of the presence of shade it is to be expected that animal production levels would be sustained for longer in this system than under braqui?ria in monoculture, hence constituting a viable alternative to recover areas suffering from the process of degradation. / Uma grande propor??o das ?reas do dom?nio da Mata Atl?ntica na Regi?o Sudeste encontrase degradada, com pouca ou nenhuma utiliza??o agr?cola, pecu?ria ou florestal. A recupera??o e desenvolvimento dessas ?reas, usando sistemas tradicionais de produ??o, contam com dois entraves principais: a declividade acentuada, que dificulta a mecaniza??o do solo e facilita a eros?o, e a baixa disponibilidade de nutrientes, principalmente de nitrog?nio. A integra??o de pastagens com ?rvores tem sido considerada uma op??o vi?vel para promover a sustentabilidade dos sistemas de produ??o animal desta Regi?o. Assim, o projeto de pesquisa visou estudar as principais vias de ingresso, perdas e transforma??es do nitrog?nio em pastagem de Brachiaria decumbens cv. Basilisk em associa??o com ?rvores (SSP) e em monocultura (BM). As esp?cies arb?reas utilizadas no SSP foram Acacia mangium, Mimosa artemisiana e Eucaliptus grandis. O experimento, localizado no Campo Experimental da Embrapa Gado de Leite, no munic?pio de Coronel Pacheco, MG, foi desenvolvido em pastagens estabelecidas em uma ?rea que apresentava grau elevado de degrada??o. Para os dois sistemas de pastejo, o delineamento experimental de blocos ao acaso, com seis repeti??es (seis piquetes de 0,5 ha) e cinco animais por piquete em sistema de pastagem rotativa. Os dados foram coletados durante um ano, observando-se, principalmente: biomassa de forragem, ganho de peso animal, consumo animal, liteira existente e depositada durante 14 dias. Considerando o total da liteira depositada, o consumo animal e a mudan?a na biomassa de forragem ao longo de 12 meses, foi poss?vel estimada produtividade prim?ria a?rea l?quida (PPAL) e a ciclagem de N nos dois sistemas de pastejo. A biomassa da forragem foi de em m?dia, 1.800 e 1.653 kg/ha nas pastagens de B. decumbens cv. Basilisk em SSP e em BM, respectivamente. Os ganhos de peso dos animais foram maiores no SSP com m?dia de 0,51 kg/novilhas/dia. Na pastagem em BM o valor m?dio foi de 0,45 kg/novilha/dia. O SSP resultou em um acr?scimo significativo na liteira depositada. C?lculos das quantidades da liteira depositada em 14 dias nas pastagens de B. decumbens cv. Basilisk em SSP e em BM, mostraram valores de 21.812 e 16.264 kg/ha/ano de mat?ria seca (MS), respectivamente. A PPAL da pastagem em SSP foi maior com valores de 26.202 e de 279 kg/ha/ano de MS e de N, respectivamente. Na pastagem em BM, a PPAL estimada foi de 20.133 e de 148 kg/ha/ano de MS e de N, respectivamente. A quantidade de N exportado da pastagem para o ganho de peso dos animais foi de 8 kg/ha/ano no SSP e 7 kg/ha/ano na BM. Os valores corresponderam a 16 e 18% de todo N consumido pelos animais nas pastagens em SSP e BM, respectivamente. As perdas de N via fezes e urina, tamb?m n?o diferiram nas duas pastagens. Na pastagem em sistema silvipastoril, devido ? fixa??o biol?gica de N2 das esp?cies de leguminosa e tamb?m devido ao efeito da sombra no bem estar dos animais, ? de esperar que a produ??o animal seja mantida por mais tempo com bases sustent?veis, sendo, portanto, uma alternativa vi?vel para recuperar ?reas em processo de degrada??o.

Rising global antibiotic resistance has caused concerns over sources and pathways for the spread of contributing factors. Majority of the antimicrobials used in the U.S. are involved in veterinary medicine, primarily with livestock rearing. Animal manure land application integrates livestock farming and agroecosystems. This manure contains antibiotic resistant elements (AREs) (resistant bacteria, resistance genes, and veterinary antibiotics) that contribute towards antimicrobial resistance. Altering manure application techniques can reduce surface runoff of other contaminants such as excess N and P, pesticides, and hormones, that can impact water quality. Conventional tillage practices in the U.S. has reduced or stopped, making subsurface injection of manure a promising option when compared to surface application. Our research compared manure application methods, manure application seasons, cropping system, and manure-rainfall time gaps to gauge the impact on AREs in the environment. Two field-scale rainfall simulation studies were conducted along with one laboratory study. Using the injection method lowered concentrations of manure associated AREs entering surface runoff. When manure was surface applied and rainfall occurred 7 d after application, 9-30 times less resistant fecal coliform bacteria (FCB) entered surface runoff when compared to 1 d time gap for that broadcast method. Within a day of manure application, antibiotic resistance gene (ARG) profiles in soil began to differ from each other based on manure application and soil ARG richness in all manure-amended soil increased compared to the background. Runoff from injection plots contained 52 ARGs with higher abundance compared to runoff from surface applied plots. ARGs in the former were more correlated to soil and more correlated to manure in the latter. The highest antibiotic concentrations were in the injection slit soil of those plots. Antibiotic concentrations in samples corresponded positively to concentrations of resistant FCB and ARGs, and there was a positive correlation between resistant FCB and their associated ARGs (Spearman's ρ = 0.43-0.63). A CRIISPR-Cas12a assay for quantification of ARGs in environmental samples was just as precise as conventional methods. There is also potential for in-situ detection. These combined results can hopefully help farmers improve manure management practices that mitigate spread of AREs to surrounding water, crops, and soil. / Doctor of Philosophy / Rising global antibiotic resistance cause concerns over sources and pathways for the spread of contributing factors. Most of the antimicrobials used in the U.S. are involved in veterinary medicine, especially with livestock rearing. Overuse of antibiotics that are medically important to human medicine compromises the effectiveness of our medicines. Animal manure contains antibiotic resistant elements (AREs) such as resistant bacteria, resistance genes, and antibiotics) that contribute towards resistance issues. Once these AREs enter the environment, they can be taken up by crops, runoff into surface water or leached into ground water, or even reside within the animal products we consume. Altering manure application techniques is beneficial for nutrient conservation but also potentially for reducing ARE spread. With our research, we compared manure application methods, manure application seasons, cropping systems, and manure-rainfall time gaps to find ways to balance the need for manure application and the spread of resistance. We used two field-scale rainfall simulation studies along with one laboratory study. Overall, using the injection method resulted in significantly lower concentrations of manure associated AREs entering surface runoff. When manure was surface applied and rainfall occurred 7 d after application, less resistant fecal coliform bacteria (FCB) entered surface runoff when compared to the 1 d time gap for broadcast methods. Within a day of manure application, antibiotic resistance gene (ARG) profiles in soil began to differ from each other and soil ARG totals in all manure applied soil increased compared to the background. Runoff from injection plots contained more soil ARGs and runoff from surface applied plots containing more manure associated ARGs. The subsurface injection method also caused highest antibiotic concentrations in the injection slit soil of those plots. High antibiotic concentrations in samples generally meant high concentrations of resistant FCB and ARGs, and resistant FCB were also found with their associated ARGs as well. A CRISPR-Cas12a assay for quantification of ARGs in environmental samples was just as precise as conventional methods. There is also potential for onsite detection. These combined results can hopefully help farmers improve manure management practices that mitigate spread of AREs to surrounding water, crops, and soil.

Widespread use of antibiotics in livestock production can result in the dissemination of bacteria carrying antibiotic resistance genes (ARGs) to the broader environment. Within agroecosystems, ARGs can pose a risk to livestock handlers, farmers, and ultimately consumers. The overall goals of this dissertation are to examine the presence of resistance (antibiotic, metal) in agricultural soils and evaluate the most critical potential points of best management control of antibiotic resistance spread along the agricultural production chain. The relative impacts of agricultural practices, manure management, native soil microbiota, and type of crop grown and harvested on the agricultural resistome are multi-dimensional and cannot be captured via a single analytical technique or by focusing on one specific point in the agricultural process. Culture-, molecular "indicator"-, and next-generation sequencing- based methods were employed to characterize antibiotic resistance via taxonomic and functional profiles on the broader manure, soil, and vegetable surface microbial communities through 16S rRNA amplicon sequencing and shotgun metagenomics. Although antibiotic concentrations dissipated in the soil after 28 days after amendment application, antibiotic resistance presence was recoverable throughout the entire 120d growing season in the compost and manure amendments, the amended soil, and on vegetable surfaces. The addition of organic fertilizers increased antibiotic resistance presence compared to background levels. Further, metals and metal resistance were also measured in the amended soils and were found to be at greater levels in the inorganically fertilized soils compared to the manures and compost amended soils. Analysis of the widespread agroecosystem microbial community composition and broader metagenome has characterized varying genera profiles in the soil and on the vegetable surfaces and specific ARG and mobile genetic element (plasmid) co-occurrences. These co-occurrences highlight which ARGs may be most critical for future antibiotic resistance dissemination research. It is imperative to employ multiple methods when measuring agricultural resistance, as one method alone may miss significant patterns and lead to different best management recommendations. Linking the livestock manure, soil, and vegetable surface-associated ARBs, ARGs, resistomes, and microbiomes will help identify critical control points for mitigation of agricultural dissemination of antibiotic resistance to the environment and food production. / Doctor of Philosophy / By 2050, it is estimated that antibiotic resistant infections will be the leading cause of death worldwide. It is important to consider human, animal, and environmental health when researching antibiotic resistance, which is known as a "One Health" approach. In this dissertation work, I focus on the environmental side of antibiotic resistance in our agricultural systems. Agriculture is a known source of antibiotic resistance due to its use of antibiotics in livestock as a treatment for illness, and in some instances, as a growth promoter. Over one growing season, I measured antibiotic resistance in an agricultural setting using many techniques. First, I analyzed the effects of inorganic (chemical) versus organic (manure and compost) fertilization on antibiotic resistance in the soil. I measured antibiotic resistance by growing antibiotic resistant bacteria, quantifying specific antibiotic resistant genes (ARGs) using DNA amplification, and quantifying all the ARGs in the soil using a next-generation sequencing (NGS) technique called shotgun metagenomics. I found that adding manure to the soil increases ARGs compared to background soil levels, and that composting in an effective management strategy in decreasing ARGs in the soil over time. Second, I analyzed the same effects of fertilization on metal resistance in the soil. I was able to use the same NGS dataset to measure metal resistance genes (MRGs). I found that adding inorganic chemical fertilizer increases MRGs in the agricultural soils compared to the organic (manure or compost) fertilizer. Additionally, I studied the microbes that live in the agricultural soils using another kind of NGS data specific for microbial identification. I found that although there were small differences between the microbial populations in the soil when fertilizers were added, they returned to similar composition over the growing season. Lastly, I measured antibiotic resistance and microbes throughout the entire agricultural system. I picked the point of fertilization (manure management), soil, and the lettuce surface to evaluate if antibiotic resistance spreads from the farm to the vegetable that ends up on a consumer's plate. I found that at each point antibiotic resistance is present, but at different levels. Composting reduces ARGs compared to raw manure. Agricultural soils may act as a natural buffer to antibiotic resistance. Lettuce plants grown in compost fertilized soils have less ARGs than lettuce plants grown in manure. There are many agricultural management practices that effectively reduce antibiotic resistance and using all of them plus many measurement methods will ultimately help farmers and consumers reduce antibiotic resistance in our agricultural systems.

Face à la mutation des modalités des échanges internationaux et à l'accentuation du commerce de biens intellectuels entre entreprises de traditions différentes, des réajustements des modèles contractuels et un réordonnancement des modes de règlement des différends semblent inévitables. La nature juridique incertaine des connaissances secrètes, en Chine et dans l’Union européenne est un obstacle à la formalisation de leur transfert et par conséquent à la définit ion d’un cadre de résolution des différends adapté. Si les propositions de lois et de direct ive se multiplient pour définir cette notion et son régime, l’approche retenue est souvent limitée à la dimension délictuelle de la divulgation de la connaissance. La recherche menée, tout en tenant compte des atteintes portées au secret, se focalise principalement sur le transfert volontaire de connaissances. Pour répondre aux besoins des acteurs du commerce international, ces opérations ne peuvent plus être limitées aux seuls contrats de « communication de savoir - faire ». Si les notions de « bien intellectuel » et de « possession intellectuelle »permettent de justifier théoriquement d’autres formes contractuelles, les droits chinois et européen se réfèrent aux cessions et licences de connaissances. Ces opérations, souvent insérées dans un ensemble contractuel complexe et réalisées entre parties de cultures différentes,peuvent engendrer des différends variés. Lorsque ces différends apparaissent,le degré de confidentialité assuré devant les juridictions nationales étudiées ne permet pas de répondre aux attentes légitimes des parties. Les différences de cultures processuelles des parties et la culture chinoise du règlement amiable des différends invitent aussi à éviter le recours aux juridictions nationales. Les combinaisons entre médiat ion et arbitrage, par leur grande flexibilité procédurale, peuvent fournir un cadre de résolut ion adapté aux exigences tant économiques que culturelles des parties. Dans cet esprit, la thèse propose la mise en place d’un Règlement de M²arb avec un médiateur–expert, garant de la confidentialité du processus de résolut ion des différends. / Facing the evolution of international trade and the development of intellectual proper ty trade between companies from different backgrounds, contractual model readjustments and a reordering of dispute resolution systems seems unavoidable. The unsettled legal status of secret knowledge in China and in the EU can be an obstacle to the formalization of their transfer and therefore inhibits the definition of a suitable framework for resolving disputes. Legislators and scholars are currently debating proposals of laws and directives to elaborate this concept and its regime, but only address issues liketor t and disclosure of secret information. Legal scholarship to date has mainly been focused on the voluntary transfer of knowledge and has taken intoaccount the damage caused to secrets. To meet the needs of business operators, these operations should not be limited to "know-how communication contracts” (contrats de communication de savoir - faire). The concepts of “ intellectual good” and " intellectual ownership" theoretically justifyother types of contracts, while Chinese and European laws refer to assignment and license of trade secret . These operations, often provided for in a complexs et of contracts between parties from different cultural backgrounds, can lead to a wide variety of disputes. When disputes occur, national courts do not always provide a level of protect ion of confidential information that meets parties’ expectations. Differences in procedural cultures as well as the Chinese culture of amicable dispute settlement prevent the parties from relying on national courts. The combinations between mediation and arbitration, by their procedural flexibility, can provide a suitable dispute resolution framework taking into account economic and cultural considerations. Therefore, this thesis proposes M² arb Rules that introduce a mediator-expert with a mission of securing knowledge confidentiality during the dispute resolution process.