Made available in DSpace on 2014-12-17T14:13:41Z (GMT). No. of bitstreams: 1 LarissaMJS_TESE.pdf: 4008472 bytes, checksum: ac86b2f6644df871874d4b2ab104f409 (MD5) Previous issue date: 2011-11-23 / Universidade Federal do Rio Grande do Norte / Farming of marine shrimp is growing worldwide and the Litopenaeus vannamei (L. vannamei) shrimp is the species most widely cultivated. Shrimp is an attractive food for its nutritional value and sensory aspects, being essential the maintenance of this attributes throughout storage, which takes place largely under freezing. The aim of this research was to evaluate quality characteristics of Litopenaeus vannamei shrimp, during freezing storage and to verify the effect of rosemary (Rosmarinus officinalis) adding. Considering the reutilization of processing shrimp wastes, total carotenoids analysis were conducted in waste of Litopenaeus vannamei shrimp and in the flour obtained after dryer. Monthly physicochemical and sensorial analysis were carried out on shrimp stored at 28,3 ? 3,8?C for 180 days. Samples were placed in polyethylene bags and were categorized as whole shrimp (WS), peeled shrimp (PS), and PS with 0,5% dehydrated rosemary (RS). TBARS, pH, total carotenoid and sensorial Quantitative Descriptive Analysis (QDA) were carried out. Carotenoid total analysis was conducted in fresh wastes and processed flour (0 day) and after 60, 120 and 180 days of frozen storage. After 180 days, RS had lower pH (p = 0.001) and TBARS (p = 0.001) values and higher carotenoids (p = 0.003), while WS showed higher carotenoid losses. Sensory analysis showed that WS were firmer although rancid taste and smell were perceived with greater intensity (p = 0.001). Rancid taste was detected in RS only at 120 days at significantly lower intensity (p = 0.001) than WS and PS. Fresh wastes had 42.74&#956;g/g of total carotenoids and processed flour 98.51&#956;g/g. After 180 days of frozen storage, total carotenoids were significantly lower than 0 day (p<0,05). The addition of rosemary can improve sensory quality of frozen shrimp and reduce nutritional losses during storage. Shrimp wastes and flour of L. vannamei shrimp showed considerable astaxanthin content however, during storage it was observed losses in this pigment / O cultivo do camar?o marinho cresce mundialmente, sendo o Litopenaeus vannamei (L. vannamei), a esp?cie mais cultivada na atualidade. O camar?o ? um alimento atrativo por seu valor nutricional e aspectos sensoriais, sendo essencial, a manuten??o desses atributos ao longo do armazenamento. Os objetivos da presente pesquisa foram avaliar caracter?sticas de qualidade do camar?o L. vannamei adicionado de alecrim (Rosmarinus officinalis) e armazenado sob congelamento, assim como realizar a determina??o da concentra??o de caroten?ides totais dos res?duos do camar?o e da farinha obtida ap?s secagem. As amostras foram colocadas em embalagens de polietileno e estocadas como Camar?o Inteiro (CI), Camar?o Descascado (CD) e Camar?o descascado adicionado de 0,5% de alecrim desidratado (CA). An?lises de TBARS, pH e caroten?ides totais foram realizadas, assim como an?lise sensorial utilizando An?lise Descritiva Quantitativa (ADQ). As an?lises f?sico-qu?micas e sensoriais do camar?o L. vannamei armazenado sob congelamento (-28,3?3,8?C), foram realizadas mensalmente, por um per?odo de 180 dias. Com rela??o ?s an?lises dos res?duos, estas foram realizadas no material in natura e na farinha rec?m-processada (dia 0) e aos 60, 120 e 180 dias de armazenamento sob congelamento. No final do per?odo de armazenamento CA apresentou menor pH (p = 0,001) e valores de TBARS (p = 0,001) e concentra??o mais alta de caroten?ides totais (p = 0,003), enquanto CI demonstrou maior perda de caroten?ides. A an?lise sensorial mostrou maior firmeza para CI, por?m estas amostras apresentaram sabor e cheiro de ran?o em maior intensidade (p = 0,001). Os provadores perceberam sabor de ran?o nas amostras CA apenas aos 120 dias de armazenamento, e em menor intensidade do que nas amostras de CI e CD (p = 0,001). No que se refere ?s an?lises dos res?duos, observou-se concentra??o de 42,74&#956;g/g de caroten?ides totais nos res?duos frescos e 98,51&#956;g/g na farinha rec?m-processada. Ap?s 180 dias de armazenamento sob congelamento, os teores de caroten?ides totais diminu?ram significativamente quando comparados ao dia 0 (p<0,05). A adi??o de alecrim pode melhorar a qualidade sensorial do camar?o congelado e reduzir perdas de seu valor nutricional ao longo do armazenamento sob congelamento. Os res?duos e a farinha do camar?o L. vannamei, apresentaram concentra??o consider?vel de astaxantina, que foi reduzida ao longo do congelamento

Made available in DSpace on 2014-12-17T15:36:56Z (GMT). No. of bitstreams: 1 PriscilaFS.pdf: 858525 bytes, checksum: 5e35b139cd8c6a2aa511bd7fc297a0d1 (MD5) Previous issue date: 2009-03-30 / Shrimp culture represents an important activity to brazilian economy. The northeastern region has presented high levels of production because of its climatic conditions. An important factor for the activity?s major development is related to the introduction of the species Litopenaeus vannamei. The use of an exotic species can disturb the ecosystem. In the last decades, L. vannamei has been the only species cultivated in Brazilian farms, there not being an alternative species for shrimp culture. So, there is an urgent need to developing new studies with the native species, which might represent an alternative concerning shrimp production, with emphasis on Farfantepenaeus subtilis. Another important aspect related to the activity is feeding management, once it is quite usual that feed offer on the pond does not take into account either the species? physiology and behavior or the influence of environmental variables, such as light cycle and substrate. That knowledge may optimize management and so reduce the impact of effluents in the environment. This study?s objective was characterizing feeding behavior of F. subtilis in laboratory. For that, an ethogram was developed, using 20 wild animals which were observed through ad libitum and all occurrences methods. Two experiments were developed in order to register feeding behavior on different substrates, along 15 days, each. In the first experiment, 40 animals were distributed in eight aquaria, half being observed during the light phase of the 24 hour cycle and the other half in the dark phase, both in halimeda substrate. In the second experiment, 20 animals were distributed in four aquaria, under similar conditions as the previous ones, but in sand substrate. In both experiments, animals were observed respectively one, four, seven and ten hours after the beginning of the phase, for light phase, for the dark phase, in ten minute observation windows, before and immediately after feed offer. The following behaviors were registered: feed ingestion, ingestion of other items, inactivity, exploration, vertical exploration, swimming, crawling, digging, burrowing, and moving by the animals. Observation windows after feed offer also included latency to reach the tray and to ingest feed. Nineteen behaviors were described for the species. F. subtilis presented more behavioral activities in halimeda substrate even in the light phase, while burrowing was predominant in sand substrate. In both substrates, moving, crawling and exploration were more frequent after feed offer, but inactivity and burrowing were more frequent before that. Feed ingestion was more frequent in halimeda, both in light and dark phases. Weight gain was also more prominent in that substrate. In sand substrtate, ingestion was more frequent in the dark phase, which suggests that higher granulometry facilitates feed ingestion in F. subtilis juveniles. Our results demonstrate the importance of studies for the better knowledge of the species, specially its response to environmental stimuli, in order to improve animal management / A carcinicultura ? uma atividade de grande import?ncia econ?mica no Brasil. Um fator que contribuiu para o crescimento da atividade foi a introdu??o da esp?cie Litopenaeus vannamei. A utiliza??o de uma esp?cie ex?tica pode causar danos ao ecossistema nativo e atualmente L. vannamei ? a ?nica esp?cie cultivada no Brasil, n?o havendo uma alternativa para o setor. Da? a necessidade de estudos com esp?cies nativas que possam representar outra via para a produ??o, em particular Farfantepenaeus subtilis. O conhecimento sobre o comportamento e fisiologia da esp?cie e sobre a influ?ncia das caracter?sticas do ambiente, como o ciclo de luz e substrato, sobre a ingest?o, pode otimizar o manejo e minimizar o impacto do despejo de efluentes no meio ambiente. Assim, esse estudo objetiva caracterizar o comportamento alimentar de F. subtilis ao longo do ciclo de luz em diferentes substratos sob condi??es laboratoriais. Para isso foi elaborado um etograma utilizando 20 animais trazidos da natureza, e observados pelos m?todos ad libitum e Todas as Ocorr?ncias. Para observa??o do comportamento alimentar dois experimentos foram realizados em diferentes substratos, com dura??o de 15 dias cont?nuos cada. No primeiro experimento 40 animais foram distribu?dos em oito aqu?rios, quatro na fase de claro e quatro na fase de escuro, com substrato de conchas de ostras trituradas (halimeda). No segundo experimento 20 animais foram distribu?dos em quatro aqu?rios, dois na fase de claro e dois na fase de escuro, com substrato de areia. Em ambos os experimentos os animais foram observados na fase de claro nos hor?rios 7h, 10h, 13h e 16h (1, 4, 7, 10) e nos hor?rios equivalentes na fase de escuro19h, 22h, 1h e 4h (1, 4, 7, 10), em janelas de 10 minutos antes e imediatamente ap?s a oferta da ra??o. Os comportamentos registrados foram: ingest?o do item ofertado, ingest?o do item que n?o o ofertado, parado, explora??o, explora??o vertical, nata??o, rastejamento, cavar, enterramento, limpeza e o deslocamento dos animais. Ap?s a oferta do alimento as lat?ncias de chegada a bandeja e de consumo do alimento foram registradas. Ao todo 19 comportamentos foram descritos para a esp?cie. F. subtilis apresentou uma maior diversidade de comportamentos na halimeda, mesmo na fase de claro, enquanto que na areia o enterramento predominou. Nos dois substratos o deslocamento, rastejamento e explora??o foram mais freq?entes ap?s a oferta do alimento, enquanto o parado e o enterramento foram mais frequentes antes. A ingest?o da ra??o foi maior no substrato de halimeda, n?o diferindo entre as fases de claro e escuro, o ganho de peso dos animais tamb?m foi maior nesse substrato. Na areia a ingest?o foi mais freq?ente na fase de escuro, o que sugere que substratos de maior granulometria favorecem a ingest?o do alimento em juvenis de F. subtilis. Nossos resultados demonstram a import?ncia do conhecimento da esp?cie e de sua resposta aos est?mulos do ambiente, como subs?dio para o desenvolvimento de estrat?gias de manejo eficientes

Made available in DSpace on 2014-12-17T15:37:19Z (GMT). No. of bitstreams: 1 EricSF.pdf: 350001 bytes, checksum: 9fc8363dd4359a5ba205b8e441f4895c (MD5) Previous issue date: 2006-06-28 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Carciniculture in Brazil occupies world-wide prominence due to shrimp culture, and the state of Rio Grande do Norte has presented the best results in the culture of the Litopenaeus vannamei in the last decade. This species has been shown to adapt easily to different environments and is between the five most cultivated penaeids of the world. The ponds are usually constructed in areas close to water courses and estuaries. Stock density and substrate ponds can pollute environment, causing losses in the growth and survival of the shrimps, being considered stress factors. Shrimps in inadequate densities and substrates can result reduced productivity of the farm; and favor diseases. So, it is important to verify how these variables influence the development of the animals in the culture farms. Our objective was to study the influence of the type of substrate and the stock density on the behavior and haemocyte count of the L. vannamei. Individually marked juvenile shrimps were kept in aquaria with 30 L of seawater and continuous aeration, in 12L-12D photoperiod. They were observed through Ad libitum and focal sampling instantaneous methods during thirty days, five times per week, six times per day (8:00 to 18:00) in windows of 15 minutes every two hours. The marking of carapace permitted quantifying molting and the feeding was supplied three times a day. Two experiments were carried out: the first one tested animals in the three different substrates (fine sand, smaller rocks-SPP and biggest rocks-SGR) with 33 shrimp/m2. In the second one, the animals were tested in three stock densities (26, 52 and 66 shrimp/m2) in fine sand substrate. At the end of experiment, biometry (first and second ones) and haemocyte count (second one) were made. The behavior of the L. vannamei seems to have been influenced by substrate and stocking density. In low granulometry of the substrate; the exploratory behavior became more frequent and inactivity of the shrimps was reduced. Burrowing was registered in sand substrate, specially in the initial period of the day. Cleaning was gradually higher along the day, presenting the biggest levels as the dark phase approached. The ingestion of feeding was more frequent in low density, and the animals were bigger and heavier at the end of the experiment. In the fine sand condition, the animals presented better growth, probably associated with the burrowing. The molting was equivalent in all types of substrate, but it was more frequent in high densities. Mortality of the shrimps was more frequent in high densities, and cannibalism and diseases were also registered in that condition. The clinical signals were similar to the ones of infectious mionecrosis (IMNV), generally associated with environment and physical stress. The haemocyte count was low for the hematologic standards of the penaeid, which we attributed for greater dilution of haemolymph in the postmolting phase. Smaller shrimps presented lower levels of haemocytes in relation to the bigger animals, count was also low in 26 shrimp/m2 density. The study demonstrates that stocking density and the granulometry of the substrate can affect the welfare, the health and the behavior of the L. vannamei. The sand substrate and low stocking density can be important tools in the management systems of shrimp production / A carcinicultura brasileira ocupa lugar de destaque mundial devido ? cria??o de camar?o, sendo o Rio Grande do Norte o maior produtor no cultivo de Litopenaeus vannamei. Essa esp?cie apresenta grande adapta??o ambiental e est? entre os cinco pene?deos mais cultivados do mundo. A cria??o ? realizada em viveiros nas ?reas pr?ximas a cursos d ?gua e estu?rios. Altas densidades populacionais e o substrato dos viveiros acarretam o aumento da polui??o e promovem perdas no crescimento e na sobreviv?ncia dos camar?es, sendo considerados fatores estressantes. Esses fatores podem comprometer a produtividade dos viveiros e favorecer doen?as, sendo importante verificar como essas vari?veis influenciam no desenvolvimento dos animais nas fazendas de cultivo. Nosso objetivo foi estudar a influ?ncia do tipo de substrato e da densidade populacional sobre o padr?o de atividades comportamentais e os n?veis de hem?citos (CHT) de L. vannamei. Em aqu?rios com 30 litros de ?gua salgada com aera??o cont?nua e em fotoper?odo 12C 12E (claro das 06:00 ?s 18:00), camar?es juvenis marcados individualmente foram observados pelos m?todos Ad libitum e focal instant?neo durante 30 dias, 5 vezes por semana, 6 observa??es di?rias (iniciando a primeira ?s 8:00 e a ?ltima ?s 18:00) em janelas de 15 minutos a cada duas horas, sendo registrado a cada minuto os seus comportamentos e localiza??o. Tamb?m foi feita a marca??o da carapa?a para quantificar as ecdises e a alimenta??o foi fornecida tr?s vezes ao dia. Dois experimentos foram realizados: tr?s substratos diferenciados (Areia, Seixos Pequenos e Seixos Grandes) com 33 animais/m2; e outro com tr?s densidades populacionais (26, 52 e 66 animais/m2) em substrato arenoso. Ao final dos experimentos, foram feitas a biometria dos camar?es e a contagem de hem?citos (apenas no 2? experimento). O padr?o geral de atividades comportamentais de L. vannamei n?o foi influenciado pelo substrato e densidade populacional. Contudo, ? medida que diminuiu a granulometria do substrato, o comportamento de explora??o tornou-se mais freq?ente e reduziu a inatividade dos camar?es. O enterramento foi registrado em substrato arenoso, sendo maior no per?odo inicial do dia. A limpeza distribuiu-se de forma crescente ? medida que a fase escura se aproximava, sendo maior ? noite. A ingest?o alimentar foi mais freq?ente em densidade baixa; acarretando maior crescimento nos camar?es. Houve maior ganho de peso nos animais em substrato arenoso, podendo estar associado com o enterramento e a taxa de crescimento dos animais. A taxa de ecdise foi igual em todos os substratos testados, mas a taxa de ecdise foi maior em maiores densidades. A mortalidade foi mais elevada em condi??es de maiores densidades, sendo registrados canibalismo e enfermidades nos animais. Os sinais cl?nicos foram semelhantes aos da mionecrose infecciosa (IMNV), geralmente associada ao estresse ambiental. Os n?veis de hem?citos foram baixos para os padr?es dos pene?deos, atribu?da pela maior dilui??o da hemolinfa na fase de p?s-ecdise. Camar?es menores apresentaram n?meros de hem?citos reduzidos em rela??o aos maiores, sendo tamb?m baixos em densidade de 26 camar?es/m2. O estudo demonstrou que os efeitos da densidade populacional elevada e a gramulometria do substrato podem afetar o bem estar, a sa?de e o comportamento de L. vannamei. O uso de substrato arenoso e baixa densidade populacional parecem ser medidas de manejo importantes em um sistema de produ??o de camar?o

Submitted by Automa??o e Estat?stica (sst@bczm.ufrn.br) on 2017-06-02T22:53:27Z No. of bitstreams: 1 SavioArcanjoSantosNascimentoDeMoraes_DISSERT.pdf: 3382313 bytes, checksum: d29b0d639c0367f1cb819ebeb2afb377 (MD5) / Approved for entry into archive by Arlan Eloi Leite Silva (eloihistoriador@yahoo.com.br) on 2017-06-09T22:09:38Z (GMT) No. of bitstreams: 1 SavioArcanjoSantosNascimentoDeMoraes_DISSERT.pdf: 3382313 bytes, checksum: d29b0d639c0367f1cb819ebeb2afb377 (MD5) / Made available in DSpace on 2017-06-09T22:09:38Z (GMT). No. of bitstreams: 1 SavioArcanjoSantosNascimentoDeMoraes_DISSERT.pdf: 3382313 bytes, checksum: d29b0d639c0367f1cb819ebeb2afb377 (MD5) Previous issue date: 2017-02-21 / Macrobrachium jelskii ? uma esp?cie de ampla distribui??o no nordeste brasileiro, e ocorrendo em toda Am?rica do Sul. Contudo, nenhum estudo acerca a filogeografia, varia??es morfol?gicas locais e variabilidade gen?tica das popula??es desta esp?cie foi efetuado. Esta esp?cie de camar?o, pode revelar informa??es importantes sobre o padr?o de dispers?o e vicariantes correlatos ? cen?rios de arranjos tect?nicos pret?ritos e outros eventos que possam ter influenciado a biogeografia dos taxa aqu?tico. Portanto, o presente trabalho tem como objetivo investigar a diversidade fenot?pica e molecular das popula??es do camar?o Macrobrachium jelskii, nas ecorregi?es Nordeste M?dio Oriental, Maranh?o Piau? e S?o Francisco, frente a influ?ncia de cen?rios pret?ritos e respostas ambientais locais. Este trabalho utilizou uma abordagem de Sistem?tica integrativa, a morfometria geom?trica multivariada, an?lises filogen?ticas e de estrutura??o gen?tica. A morfometria geom?trica, revelou dimorfismo sexual evidente, em que f?meas apresentaram cefalot?rax e abd?men mais estirados horizontalmente. Isto, possivelmente, favorece o desenvolvimento gonadal, vitelog?nese e armazenamento dos ovos. Abstra??o da forma dos tr?s planos corporais n?o demostrou um consenso acerca da estrutura??o das popula??es entre de M. jelskii no Nordeste Brasileiro. Tal fato corrobora com a baixa diversidade nucleot?dica e haplot?pica, em que foi relatado apenas um hapl?tipo compartilhado entre as popula??es das bacias. Assim, ratificando a possibilidade de introdu??o da esp?cie nos corpos d??gua do Nordeste pelo DNOCS. Em adi??o, os dados gen?ticos relataram a presen?a de ru?dos e picos duplos no eletroferograma, que representa co-amplifica??o de pseudogenes mitocondriais da regi?o Citocromo Oxidase I, pouco relatadas na literatura para o g?nero Macrobrachium. Este fato abre um precedente de desconfian?a para rela??es filogen?ticas e filogeogr?ficas publicadas, recentemente que n?o retratam isto. Sabendo que esta caracter?stica pode gerar uma superestima??o de linhagens evolutivas e, assim, conclus?es err?neas sobre eventos dispersivos, vicariantes ou de especia??o, se faz de suma import?ncia retratar os casos de pseudogenes e a utiliza??o de iniciadores espec?ficos para cada t?xon. / Macrobrachium jelskii is a widely distributed species in northeastern Brazil, occurring throughout South America. However, no studies on phylogeography, local morphological variations and genetic variability of the populations of this species were carried out. This prawn species can reveal important information on the dispersion pattern and related vicariates for scenarios of past tectonic arrangements and other events that have influenced the biogeography of the aquatic taxa. The aim of this manuscript is to investigate a phenotypic and molecular diversity of the prawn M. jelskii populations in the Mid-Northeast Caatinga, Maranho Piau? and S?o Francisco ecoregions, influenced by past scenarios and environmental local responses. This scientific article uses an approach of Integrative Systematics, a multivariate geometric morphometry, phylogenetic analyzes and genetic structuring. Geometric morphometry showed evident sexual dimorphism, in which females presented cephalothorax and abdomen more horizontally stretched. This possibly favors gonadal development, vitellogenesis and egg storage. Abstraction of the shape of the three body planes does not demonstrate a consensus on the structuring of the populations between M. jelskii in the Northeast of Brazil. This fact corroborates a low nucleotide and haplotype diversity, in which only one haplotype was shared among the populations of the basins. Thus, ratifying a possibility of introduction in the Northeast area by DNOCS. In addition, the genetic data reported a presence of noises and double peaks not electropherogram, which represents co-amplification of mitochondrial pseudogenes of the region Cytochrome Oxidase I, little reported in the literature for the genus Macrobrachium. This fact show a precedent of mistrust for published phylogenetic and phylogeographic relationships, which recently did not depict this. Knowing that this characteristic can generate an overestimation of evolutionary lineages and, thus, erroneous conclusions on dispersive, vicarious or speciation events, it is of importance to report the cases of pseudogenes and the use of specific molecular primers for each taxon.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-07-24T16:03:54Z No. of bitstreams: 1 2009 - Helaine dos Reis Flor.pdf: 3447034 bytes, checksum: 3861cf7664eb46a21add49a26a4479c2 (MD5) / Made available in DSpace on 2017-07-24T16:03:54Z (GMT). No. of bitstreams: 1 2009 - Helaine dos Reis Flor.pdf: 3447034 bytes, checksum: 3861cf7664eb46a21add49a26a4479c2 (MD5) Previous issue date: 2009-09-01 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / Farfantepenaeus brasiliensis is a native species also known as the pink shrimp, and is one of the most explored fishing resources of the Brazilian coastline. This study aims to evaluate the reproductive performance of the F. brasiliensis raised in captivity submitted to different diets and sex ratios. Wild broodstock were captured in the Ba?a de Sepetiba, near to the Restinga da Marambaia and were kept for 107 days in maturation tanks at the Esta??o de Biologia Marinha da Universidade Federal Rural do Rio de Janeiro (EBM-UFRRJ). After ten days of acclimatization, the animals were randomly assigned to 18 polyethylene tanks, 450L volume, with constant aeration, continuous filtered seawater exchange, feeding trays and pieces of PVC pipes to serve as shelter. Each tank stored six animals. The experimental design was completely randomized with six treatments and three repetitions for each. Three diet feeding protocols were evaluated: A- 25% commercial diet /75% fresh food; B- 50% commercial diet 50% fresh food e C- 75% commercial diet /25% fresh food and two sex rations (male:female) 1:1 and 1:2 were evaluated. Females shrimp were unilaterally eyestalk ablated and marked with alternate cuts on the uropods for individual identification. The number of spawns, fecundity and hatching rate were used as the criteria for evaluation of reproductive performance. The 1M:2F sex ratio and the B feeding protocol had the best results regarding reproductive performance related to number of eggs/female and total egg production. Treatments that used the C diet presented the best hatching rates, despite of the low number of spawns. Throughout the experiment moulting occurred, however the spawning stopped between the 81st and the 90th day, probably due to physiological stress. At the end of the experiment, the females presented low survival rate in every treatment, possibly due to handling stress occurred during the gonadal maturation checks. Overall results of the present study indicated that it is possible to reproduce the F. brasiliensis in captivity, in small tanks, showing satisfactory reproductive performance. Nevertheless, it is necessary to re-evaluate the animal handling, in order to decrease the mortality and optimize the performance in captivity. / Farfantepenaeus brasiliensis ? uma esp?cie nativa conhecida como camar?o-rosa, e um dos recursos pesqueiros mais freq?entes e explorados na costa brasileira. Este trabalho teve como objetivo avaliar o desempenho reprodutivo de F. brasiliensis em cativeiro submetidos a diferentes dietas e propor??es sexuais. Os reprodutores selvagens foram capturados na Ba?a de Sepetiba, pr?ximo ? Restinga da Marambaia e mantidos durante 107 dias em tanques de matura??o na Esta??o de Biologia Marinha da Universidade Federal Rural do Rio de Janeiro (EBM-UFRRJ). Ap?s dez dias de aclimata??o, os animais foram distribu?dos aleatoriamente em 18 tanques de polietileno com volume ?til de 450L, com aera??o constante, fluxo cont?nuo de ?gua do mar filtrado, bandejas de alimenta??o e peda?os de canos de PVC para servir de abrigo para os animais. Em cada tanque foram colocados 6 animais. O delineamento experimental foi inteiramente casualizado com seis tratamentos e tr?s repeti??es cada. Foram avaliadas tr?s dietas alimentares: A-25% ra??o/75% alimento fresco; B-50% ra??o/50% alimento fresco e C-75% ra??o/25% alimento fresco e duas propor??es sexuais (macho:f?mea) de 1:1 e 1:2.Todas as f?meas foram abladas e marcadas com cortes alternados nos ur?podos para identifica??o individual. A avalia??o do desempenho reprodutivo foi realizada atrav?s do n?mero de desovas, fecundidade e taxa de eclos?o de cada tratamento. A propor??o sexual 1M:2F e a dieta B apresentaram os melhores resultados de desempenho reprodutivo em rela??o ao no de desovas e fecundidade. Os tratamentos que receberam a dieta C foram os que apresentaram melhor taxa de eclos?o, apesar do baixo n?mero de desovas. Ocorreram mudas durante todo per?odo experimental, por?m a ocorr?ncia das desovas cessou entre 81o e 90o dia de experimento, provavelmente por desgaste fisiol?gico. Ao final do experimento, as f?meas apresentaram alta mortalidade em todos os tratamentos, possivelmente devido ao estresse causado pelo manuseio di?rio para vistoria da matura??o das g?nadas. Os resultados obtidos no presente estudo constataram a possibilidade de F. brasiliensis reproduzir em cativeiro em tanques pequenos, apresentando desempenho reprodutivo satisfat?rio. Por?m ? necess?rio reavaliar o manejo, para diminuir a mortalidade e melhorar a efici?ncia de c?pula, para otimizar o desempenho em cativeiro.

Made available in DSpace on 2014-12-17T15:42:16Z (GMT). No. of bitstreams: 1 GeovaneCC_DISSERT.pdf: 2255225 bytes, checksum: bbeea0560f32e4ce6bfbdaafa5961d9f (MD5) Previous issue date: 2014-06-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / In this study, we worked with the validation of a methodology for analysis of bioactive amines in shrimp, considering it to be one of the main products of the northriograndense trade balance, maintaining the state of Rio Grande do Norte topped the list of Brazilian exports of this product the last decade. The sector of the Brazilian shrimp works exclusively with gray shrimp Litopenaeus Vannamei since the late 1990s. This study used liquid chromatography with conductimetric detector, using as the mobile phase methylsulfonic 3 mM acid (MSA) with gradient and phase C18 column with reverse the development of methodology for the analysis of bioactive amines in shrimp. In the sample preparation was used as 5% trichloroacetic acid (TCA) extraction solution. Validation analysis of biotativas amines (putrescine - PUT, histamine - HIST, agmatine - AGM, spermidine - EPD and spermine - EPN) in shrimp, the linear working range was 0.1 to 2.0 mg L-1 to was sensitive, homoscedastic, in effect, selective, accurate and precise array. Thus, considered feasible for these determinations bioactive amines in this array. Determined the concentration of these amines in fresh shrimps (AGM = 0.61 ? 0.05 mg kg- 1 EPD = 2.57 ? 0.14 mg kg-1 and EPN = 1.79 ? 0.11 mg kg-1), and freezing weather predetermined in cooked shrimp (AGM = 6.28 ? 0.18 mg kg-1, EPD = 12.72 ? 0.02 mg kg- 1 and EPN = 22.30 ? 0.60 mg kg-1), the shrimp with twenty-four hour stay at room temperature (PUT = 879.52 ? 28.12 mg kg-1, AGM = 848.13 ? 19.40 mg kg-1, ESPD = 13.59 ? 0.97 mg kg-1 and ESPN = 18.47 + 1.57 mg kg-1). In shrimp subjected to freezing for a week, two weeks, three weeks and four weeks, the results showed that there is an increase in the content of agmatine (7.31 ? 0.21 mg kg-1) while in spermine ( 1.22 ? 0.14 mg kg-1) and spermidine (below limit of quantification) there was a decrease in the freeze time, while there is a decrease in the level of spermidine not reaching detectad. The putrescine was only found in shrimp that remained for 24 hours at room temperature and histamine was not found in any of the samples / Neste trabalho trabalhou-se com a valida??o de uma metodologia para an?lise de aminas bioativas em camar?o, considerando ser este um dos principais produtos da balan?a comercial norte-riograndense, mantendo o Estado do Rio Grande do Norte no topo da lista de exporta??o brasileira deste produto na ?ltima d?cada. O setor da carcinicultura brasileira trabalha exclusivamente com o camar?o cinza Litopenaeus Vannamei desde o final da d?cada de 1990. Este trabalho utilizou a cromatografia l?quida com detector condutim?trico, usando como fase m?vel o ?cido metilsulf?nico 3 mM (MSA) com gradiente e coluna C18 com fase reversa para o desenvolvimento da metodologia para an?lise de aminas bioativas em camar?o. Na prepara??o da amostra utilizou-se como solu??o extrativa ?cido tricloroac?tico a 5% (TCA). Na valida??o da an?lise das aminas biotativas (putrescina - PUT, histamina - HIT, agmatina - AGM, espermidina - EPD e espermina - EPN) em camar?o, a faixa linear de trabalho foi de 0,1 a 2,0 mg L-1 que se mostrou sens?vel, homosced?stico, com efeito de matriz, seletivo, exato e preciso. Dessa maneira, considerada vi?vel para determina??es dessas aminas bioativas nessa matriz. Determinou-se a concentra??o destas aminas em camar?es frescos (AGM = 0,61 ? 0,05 mg Kg-1, EPD = 2,57 ? 0,14 mg Kg-1 e EPN = 1,79 ? 0,11 mg Kg-1), e com tempo de congelamento pr?-determinados, no camar?o cozido (AGM = 6,28 ? 0,18 mg Kg-1, EPD = 12,72 ? 0,02 mg Kg-1 e EPN = 22,30 ? 0,60 mg Kg-1), no camar?o com vinte e quatro horas de perman?ncia ? temperatura ambiente (PUT = 879,52 ? 28,12 mg Kg-1 , AGM = 848,13 ? 19,40 mg Kg-1, EPD = 13,59 ? 0,97 mg Kg-1 e ESPN = 18,47 + 1,57 mg Kg-1). No camar?o submetido a congelamento por uma semana, por duas semanas, por tr?s semanas e por quatro semanas, os resultados apresentaram que h? um aumento no teor de agmatina (7,31 ? 0,21 mg Kg-1) enquanto que na espermina (1,22 ? 0,14 mg Kg-1) e na espermidina (menor que o limite de quantifica??o) o houve uma diminui??o com o tempo de congelamento, enquanto que h? diminui??o no teor de espermidina chegando a n?o ser detectad. A putrescina s? foi encontrada no camar?o que permaneceu por 24 horas ? temperatura ambiente e a histamina n?o foi encontrada em nenhuma das amostras analisadas

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-08-24T18:39:52Z No. of bitstreams: 1 2008 - Cristiane Honorato Cunha.pdf: 767814 bytes, checksum: 805ea6db9e6a4462ae4375d06559e0fd (MD5) / Made available in DSpace on 2018-08-24T18:39:52Z (GMT). No. of bitstreams: 1 2008 - Cristiane Honorato Cunha.pdf: 767814 bytes, checksum: 805ea6db9e6a4462ae4375d06559e0fd (MD5) Previous issue date: 2008-03-25 / Macrobrachium acanthurus is the freshwater prawn species that is find in almost whole coastal rivers in Brazilian coast. This work was carried out to get information about reproductive aspects of Macrobrachium acanthurus in captivity and the influence on unilateral eyestalk ablation technique. 48 females and 24 males were captured in Sahy river at Mangaratiba/RJ and kept in adaptation for 15 days. Then, the animals carapace length and total length were measured. They were distributed into 20 liters aquariums, each of them with two males and four females. Two females in each aquarium were ablated. Abiotic factors such as pH, ammonia, nitrite and oxygen levels were checked weekly and the water temperature daily. Every day the occurrence of ovigerous females were checked in each aquarium. After three days incubating, the ovigerous females were separated until the larvae eclosion. And then, they were returned to the aquarium for a new phase of reproduction. The animals were fed with ration pellets and pieces of fish. The U test (Mann-Whitney) showed a significant difference among laying intervals between the ablated and non-ablated females. The Pearson correlation showed temperature influence on incubation period on non-ablated females, but on ablated females there was no dependency relationship. The t-test showed no significant difference on fertility, between ablated and non-ablated M. acanthurus. / Macrobrachium acanthurus ? uma esp?cie de camar?o de ?gua doce encontrado em quase todos os rios litor?neos da costa brasileira. Este trabalho foi realizado com o objetivo de obter informa??es sobre a influ?ncia da t?cnica de abla??o unilateral do ped?nculo ocular na reprodu??o de Macrobrachium acanthurus em cativeiro. Foram utilizados no experimento 48 f?meas e 24 machos, que foram coletados no Rio Sahy, Mangaratiba/RJ e foram mantidas durante 15 dias para a adapta??o. Posteriormente, os animais foram medidos em rela??o ao comprimento da carapa?a e comprimento total, distribu?dos na propor??o de dois machos para quatro f?meas em doze aqu?rios com capacidade de 20 litros. Das quatro f?meas de cada aqu?rio, duas foram abladas. Os fatores abi?ticos como o pH, am?nio, nitrito e oxig?nio dissolvido foram verificados semanalmente e a temperatura da ?gua diariamente. Todos os dias foram verificados a ocorr?ncia de exterioriza??o dos ovos em cada f?mea. As f?meas ov?geras ap?s tr?s dias de incuba??o foram individualizadas at? a eclos?o das larvas. Ap?s a eclos?o das larvas, as f?meas retornaram para o aqu?rio para uma nova fase de reprodu??o. Os animais foram alimentados com ra??o peletizada e peixe fresco. Atrav?s do teste U (Mann-Whitney) foi verificado que houve diferen?a significativa para o intervalo entre as desovas entre f?meas abladas e n?o abladas. Atrav?s da Correla??o de Pearson verificou-se que houve influ?ncia da temperatura no tempo de incuba??o nas f?meas n?o abladas, mas nas f?meas abladas n?o houve rela??o dependente. Atrav?s do teste t foi verificado que n?o houve diferen?a significativa para fertilidade entre as f?meas abladas e n?o abladas de M. acanthurus.

Made available in DSpace on 2014-12-17T14:01:58Z (GMT). No. of bitstreams: 1 MargaridaLMNS.pdf: 1054716 bytes, checksum: bc213979524001331468154edb0d566e (MD5) Previous issue date: 2008-02-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This work aimed to study the structure and dynamic of Phytoplankton and Bacterioplankton in a complete cycle of shrimp cultivation (Litopenaeus vannamei) and determine the environmental factors responsible for the structural changes of these communities. The study was realized in a saltwater shrimp farm (Maca?ba, RN), between September/2005 and February/2006, and in a freshwater shrimp farm (Cear? Mirim, RN), between May/2007 and September 2007. The samplings were collected weekly in saltwater farm and every fifteen days in freshwater farm. Total phosphorus, chlorophyll a and environmental parameters (pH, dissolved oxygen, salinity, temperature, depth and water transparency) were measured. Qualitative and quantitative analysis of the phytoplankton and bacterioplankton were carried out. The Shannon-Wiener ecologic indexes of diversity and the Pielou equitability indexes were calculated to the phytoplankton. Bacterial density was determined by epifluorescence microscopy. The data were statistically analyzed by Pearson correlation and t-Test. Chlorophycea were predominat in salt water and in the captation/drainage point (24 to 99%). Diatoms had higher wealth. The species Choricystis minor had the highest occurrence (100%) and dominance (90-100%), thus showing its adaptation to the high temperatures, salinity and low water transparency conditions. Filamentous Cyanobacteria like Oscillatoria sp., Pseudoanabaena sp. and Phormidium sp. had constant levels. The negative correlation between chlorophycea and water transparency, and the positive correlation between chlorophyll a and salinity, showed that the phytoplankton was well adapted to the low transparency and to the high salinity. The bacterioplankton was negatively correlated with the total phosphorus and salinity. In freshwater, Cyanobacteria were predominant (>80%), presenting some producers of toxins species like Microcystis sp., Aphanizomenon sp., Cylindrospermopsis raciborskii e Anabaena circinalis. Cyanobacterial density and total phosphorus and chlorophyll a concentrations exceeded the maximum value allowed by legislation. The means of total phosphorus varied from 264 to 627 Wg.L-1 and the means of chlorophyll a oscillated between 22 and 182 Wg.L-1. The phytoplankton species were selected by low availability of the light, high pH, temperature and high availability of total phosphorus. The bacterioplankton showed high densities (5,13 x 107 to 8,50 x107 Bac.mL-1). The studied environments (ponds and rivers) presented a high level of trophic state based on the high concentrations of chlorophyll a and total phosphorus and cyanobacteria dominance. The composition of species in the ponds and rivers was similar, as well as high concentrations of total phosphorus and chlorophyll a, highlighting the pollution caused by the discharges of the farms in natural environment / O presente trabalho teve como objetivo estudar a estrutura e din?mica do fitopl?ncton e bacteriopl?ncton em ciclos completos de cultivos de camar?o (Litopenaeus vannamei), determinar os fatores ambientais respons?veis pelas mudan?as estruturais destas comunidades. O estudo foi realizado em duas fazendas de camar?o: uma de ?gua salgada (Maca?ba, RN) entre setembro de 2005 a fevereiro de 2006 e outra de ?gua doce (Cear? Mirim, RN), durante o per?odo de maio de 2007 a setembro de 2007. As coletas tiveram periodicidade semanal no viveiro de ?gua salgada e quinzenal no viveiro de ?gua doce. Foram determinadas clorofila-a e f?sforo total e par?metros ambientais foram medidos (pH, oxig?nio dissolvido, salinidade, temperatura, transpar?ncia e n?vel da ?gua). An?lises qualiquantitativas do fitopl?ncton e bacteriopl?ncton foram realizadas. Os ?ndices ecol?gicos de diversidade de Shannon-Wiener e equitabilidade de Pielou foram calculados para a comunidade fitoplanct?nica. A densidade bacteriana foi realizada por microscopia de fluoresc?ncia. Os dados foram analisados estatisticamente atrav?s da correla??o de Pearson e teste t. No viveiro de ?gua salgada e no ponto de capta??o-drenagem as clorof?ceas foram dominantes (24 % -99%) e as diatom?ceas tiveram maior riqueza. A esp?cie Choricystis minor foi a esp?cie de maior ocorr?ncia (100%) e domin?ncia (90-100%), mostrando-se bem adaptada a condi??es de altas temperaturas e salinidade e baixa transpar?ncia da ?gua. Cianobact?rias filamentosas como Oscillatoria sp., Pseudoanabaena sp. e Phormidium sp. foram constantes. A correla??o negativa, entre o grupo clorof?cea e a transpar?ncia, e positiva, entre clorofila-a e salinidade, demonstrou que o fitopl?ncton esteve bem adaptado ? baixa transpar?ncia e elevada salinidade. O bacteriopl?ncton correlacionou-se negativamente com a salinidade e f?sforo total. No viveiro de ?gua doce, cianobact?rias foram dominantes (>80%), apresentando algumas esp?cies produtoras de toxinas como Microcystis sp., Aphanizomenon sp., Cylindrospermopsis raciborskii e Anabaena circinalis. A densidade de c?lulas de cianobact?rias, os n?veis de f?sforo e clorofila-a excederam o valor m?ximo permitido pela legisla??o. Os valores m?dios do f?sforo total nos ambientes estudados foram de 264 a 627 Wg.L-1e para clorofila-a foi de 22 a 182 Wg.L-1. As esp?cies do fitopl?ncton foram selecionadas pela baixa disponibilidade de luz, alto pH, temperatura e elevada disponibilidade de f?sforo total. O bacteriopl?ncton apresentou elevada densidade (5,13 x107 a 8,50 x107 Bac.mL-1). Os ambientes estudados (viveiros e rios) configuram elevado estado de trofia, com base nas altas concentra??es de clorofila-a e f?sforo total e domin?ncia de cianobact?rias. A composi??o de esp?cies nos viveiros e nos rios (ponto de capta??o/drenagem) foi semelhante, assim como altos valores de concentra??es de f?sforo e clorofila-a, evidenciando o efeito poluidor das descargas dos viveiros no ambiente natural

Made available in DSpace on 2014-12-17T14:02:03Z (GMT). No. of bitstreams: 1 PedroHOC.pdf: 308732 bytes, checksum: f9e88debb287b72111d2b3aeb9898ae7 (MD5) Previous issue date: 2006-05-19 / The objective of the current study was to evaluate the zootechnical performance (survival and growth) of Litopenaeus vannamei post-Iarvae fed an artificial shrimp diet supplemented with Artemia flakes or freeze-dried Artemia embryos. For that purpose, 20 culturing units were individually stocked with 50 shrimp post-Iarvae (average dry weight of 0,3 ? 0,03 mg) at a stocking density of 20 post-larvae per liter, and fed the experimental diets to satiation during 20 days. The experimental design consisted of four diets (T1, T2, T3 and T4) with five repetitions each. For treatments T1, T2 and T3, dietary supplements of 5mg of Artemia flakes (T1), freeze-dried Artemia embryos (T2), and of the commercial shrimp diet (T3) were offered 2 hours after the shrimp were initially fed the commercial shrimp diet. For treatment T4 (control), no additive was offered 2 hours after the initial feeding. Shrimp survival, absolut (GPA) and relative increase in weight (GPR), and specific growth rate (TCR) were used as evaluation criteria. After the experimental period, no significant statistical differences (p>0,05) in survival were observed. Regarding growth, the dietary treatment which used freeze-dried Artemia embryos as an additive (T2) presented the best results for GPA (6,7 ? 0,7 mg). There were no statistical differences within treatments T1, T3 and T4 (p>0,05). AIso, post-larvae fed freeze-dried embryos (T2) showed a relative increase in weight (2241,4%) which differed significantly (p<0,05) from T4(1911,7%) but not from T1 (1801,6%) or T3 (1946,7%). In conclusion, the results of the current study indicate that an artificial shrimp diet supplemented with freeze-dried Artemia embryos fulfils the nutritional requirements of post-larvae L. vannamei and promotes a better growth than diets not supplemented with Artemia flakes / O presente trabalho teve por objetivo avaliar o desempenho zoot?cnico (sobreviv?ncia e crescimento) de p?s-larvas de Litopenaeus vannamei alimentadas com dietas comerciais suplementadas com flocos ou embri?es liofilizados de Artemia. Para isso, durante um per?odo de 20 dias, cada uma das 20 unidades de cultivo foram estocadas com 50 p?s-larvas de peso seco m?dio inicial de 0,3 ? 0,03 mg (a uma densidade de 20 p?s-larvas por litro) e alimentadas de modo ad libitum. O desenho experimental foi inteiramente casualizado com quatro tratamentos (T1, T2, T3 e T4) com cinco r?plicas cada. Para os tratamentos T1, T2 e T3, 2 h ap?s a oferta inicial da ra??o comercial, um suplemento adicional de 5mg aditivo era ofertado. Os aditivos utilizados consistiram de Artemia em flocos (T1), embri?es liofilizados de Artemia (T2) e ra??o comercial (T3). Para o tratamento T4 (controle), n?o houve oferta de aditivo 2 h ap?s o arra?oamento. O protocolo experimental utilizou como crit?rios de avalia??o a sobreviv?ncia, o ganho de peso absoluto (GPA), o ganho de peso relativo (GPR) e a taxa de crescimento relativo (TCR). Ap?s o per?odo experimental, n?o foram observadas diferen?as estat?sticas significativas (p>0,05) para o crit?rio sobreviv?ncia. Para o crit?rio crescimento, o tratamento que utilizou embri?es de Artemia como aditivo (T2) para p?s-larvas de camar?o L. vannamei apresentou o melhor desempenho para o GPA (6,7 ? 0,7 mg). Os tratamentos T1, T3 e T4 n?o diferiram estatisticamente (p>0,05) entre si. Igualmente, as p?s-larvas alimentadas com embri?es liofilizados (T2) apresentaram ganho de peso relativo (GPR) de 2241,4%, o qual diferiu significativamente (p<0,05) do tratamento T4 (1911,7%), mas n?o dos tratamentos T1 (1801,6%) ou T3 (1946,7%). Em conclus?o, os resultados do presente estudo indicam que uma dieta artificial suplementada com embri?es liofilizados de Artemia atende ?s necessidades nutricionais das p?s-larvas de L. vannamei e promove um melhor crescimento em rela??o a dietas comerciais n?o suplementadas ou parcialmente suplementadas com flocos de Artermia

Made available in DSpace on 2014-12-17T14:01:55Z (GMT). No. of bitstreams: 1 AlexandraRSF.pdf: 554056 bytes, checksum: fdede8a12e56bfeb141534a40ac27701 (MD5) Previous issue date: 2007-12-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The integrated culture of seaweed and aquatic animals is an ancient practice in Asian countries. The expansion of this practice to western countries is consequence of the recognition of this system as a sustainable alternative that allows economical diversification and mitigation of environmental impacts generated by effluents of aquaculture. This study evaluated the growth of the seaweed Gracilaria caudata and of the shrimp Litopenaeus vannamei in monoculture (shrimps) and integrated culture (shrimps and algae) systems, and accessed the effect of the seaweed in the water quality. There were two treatments in the experiment: monoculture (shrimps) and integrated culture (shrimps/ algae). The organisms were cultured in 6 aquaria (10L) filled with seawater (35.0?0.0 PSU and 28.1?0.4?C) for 28 days. The nutrients of water (PO43-, NH4+, NO2-, NO3- and DIN), the biomass and the relative growth rate (RGR, % day-1) of seaweed and shrimps were measured weekly. The parameters pH, temperature, salinity and dissolved oxygen were measured daily. The concentration of NH4+ in integrated culture (62.8?25.2?M) was lower (Mann-Whitney p<0.001) than in monoculture (85.6?24.3?M). The mean of PO4- in monoculture (10.4?4.6?M) was markedly higher (Mann-Whitney; p=0.024) than that in integrated culture (8.7?4.1?M). The level of dissolved oxygen in integrated culture (6.0?0.6mg/L) was higher (t-Student; P=0.014) than that in shrimp monoculture (5.8?0.6mg/L). The mean values of the parameters pH, NO2-, NO3- and DIN were 7.5?0.2, 10.1?12.2?M, 24.5?3.2?M and 120.17?30.76?M in monoculture, and 7.5?0.2, 10.5?13.2?M, 27.4?3.5?M and 100.76?49.59?M in integrated culture. There were not differences in these parameters between treatments. The biomass and RGR of seaweed reached 15.0?1.9g and 7.4?2.8% day-1 at the end of the experiment. The performance of shrimp was favorable in monoculture (1.5?0.8g; 5.7?1.6% dia-1) and in integrated culture (1.5?0.7g; 5.2?1.2% dia-1), and the rate of survival was 100% in both treatments. The tolerance and favorable performance of Gracilaria caudata suggest that this seaweed might be integrated into shrimp (Litopenaeus vannamei) culture systems / O uso de macroalgas em cultivo integrado com animais ? uma pr?tica antiga em pa?ses asi?ticos. A expans?o desta pr?tica em pa?ses ocidentais ? conseq??ncia do reconhecimento desse sistema como uma alternativa que possibilita a diversifica??o econ?mica e a mitiga??o dos impactos gerados pelos efluentes da aq?icultura. Este estudo avaliou o crescimento da macroalga Gracilaria caudata e do camar?o Litopenaeus vannamei em monocultivo (camar?o) e em cultivo integrado, observando o efeito das algas na qualidade da ?gua. O experimento foi constitu?do por dois tratamentos (triplicata), realizados em aqu?rios de vidro contendo ?gua do mar (35,0?0,0 PSU e 28,1?0,4?C) durante 28 dias: monocultivo (camar?es) e cultivo integrado (camar?es/algas). Os nutrientes da ?gua, a biomassa e as taxas de crescimento relativo (TCR,%dia-1) das duas esp?cies foram determinadas semanalmente. Os par?metros f?sico-qu?micos da ?gua (pH, temperatura, salinidade e oxig?nio dissolvido) foram monitorados diariamente. Os n?veis do ?on am?nio foram significativamente menores (Mann-Whitney p<0,001) no cultivo integrado (62,8?25,2?M) do que no monocultivo (85,6?24,3?M). Este mesmo modelo foi observado para o ortofosfato, cujas m?dias foram 10,4?4,6?M no monocultivo e 8,7?4,1?M no cultivo integrado (Mann-Whitney; p=0,024). O oxig?nio dissolvido no cultivo integrado (6,0?0,6mg/L) foi maior (t-Student; P=0,014) que no monocultivo (5,8?0,6mg/L). Os par?metros pH, nitrito, nitrato e nitrog?nio inorg?nico dissolvido n?o apresentaram diferen?a entre os tratamentos, cujas m?dias foram 7,5?0,2, 10,1?12,2?M, 24,5?3,2?M e 120,17?30,76?M no monocultivo e 7,5?0,2, 10,5?13,2?M, 27,4?3,5?M e 100,76?49,59?M no integrado. A alga apresentou aumento significativo de biomassa (15,0?1,9g) e de TCR (7,4?2,8% dia-1). Os camar?es mostraram bom desempenho no monocultivo (1,5?0,8g; 5,7?1,6% dia-1) e no integrado (1,5?0,7g; 5,2?1,2% dia-1) e 100% de sobreviv?ncia, n?o havendo diferen?a entre ambos (t-student; p>0,05). A toler?ncia e o bom desempenho de Gracilaria caudata em ambientes eutr?ficos sugerem que essa alga pode ser integrada em sistemas de cultivo de camar?es (Litopenaeus vannamei)