Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-24T14:13:56Z No. of bitstreams: 1 2015 - Sandra Maria Morgado Ferreira.pdf: 5136516 bytes, checksum: e234c7bb0d1e27b7d6b27300db893f18 (MD5) / Made available in DSpace on 2017-01-24T14:13:56Z (GMT). No. of bitstreams: 1 2015 - Sandra Maria Morgado Ferreira.pdf: 5136516 bytes, checksum: e234c7bb0d1e27b7d6b27300db893f18 (MD5) Previous issue date: 2015-09-30 / This research was conducted at the Graduate Program in Biotechnology of the Northeast Biotechnology Network - (PPGB - RENORBIO), Pernambuco, at Federal Rural University of Pernambuco, focusing graduate students of agriculture and animal husbandry research area during the period 2011-2014, as well as coordinators and teachers from the same area. The main objective was to demonstrate the impact of the former students performance in Pernambuco state. In order to complete this research objective, it was adopted a methodology of data collection in documentation research and descriptive field research. The investigative tools were semi-structured interviews; survey of Lattes curriculum and records and official documents of the graduated students of the program and the biotechnology area. The quantitative analysis of the collected data was conducted through programs like Excel, Adobe InDdesign and Adobe Illustrator. The qualitative analysis was performed by categorical analysis technique where the information is grouped by categories. The study was carried out on the profile of graduated students and their contribution for the development of the Pernambuco state, as well as the perception of the coordinators and teachers about the social and economic impact of the program for the state. The results showed the relevance of the program referred to the training of qualified personnel; to increase the scientific publication with the dissemination of new knowledge to society in general. Also, it was demonstrated the social and economic benefits for the people with new techniques, processes and products generated through the results of students' surveys / Esta pesquisa foi realizada no Programa de P?s-Gradua??o em Biotecnologia da Rede Nordeste de Biotecnologia (PPGB - RENORBIO), Ponto Focal Pernambuco, na Universidade Federal Rural de Pernambuco, cujo universo de estudo foram os egressos da ?rea de concentra??o em agropecu?ria, durante o per?odo 2011-2014, bem como os coordenadores e professores da mesma ?rea. O objetivo principal foi demonstrar o impacto da atua??o dos egressos do PPGB ? RENORBIO no Estado de Pernambuco. A fim de atingir seus objetivos, foi adotada, como metodologia de coleta de dados, a pesquisa documental e a pesquisa de campo descritiva, cujas ferramentas investigativas foram: entrevistas semiestruturadas; levantamento dos curr?culos Lattes e fichas de registros de matr?cula dos egressos e documentos oficiais do programa e da ?rea de biotecnologia da CAPES. A an?lise quantitativa dos dados coletados foi realizada nos programas Excel, Adobe InDdesign e Adobe Illustrator. Para a an?lise qualitativa optou-se pela utiliza??o da t?cnica da an?lise categorial, onde as informa??es s?o agrupadas por categorias. O estudo foi desenvolvido sobre o perfil dos alunos egressos e a contribui??o da atua??o dos mesmos para o desenvolvimento do Estado de Pernambuco, bem como a percep??o dos coordenadores e professores quanto ao impacto social e econ?mico do programa para o estado. Como resultado, constatou-se a relev?ncia do programa para a forma??o de pessoal qualificado; o aumento do acervo bibliogr?fico com a divulga??o de novos conhecimentos para a sociedade em geral; e os benef?cios sociais e econ?micos gerados ? popula??o com as novas t?cnicas, processos e produtos oriundos da aplica??o pr?tica dos resultados das pesquisas dos egressos

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-24T16:23:42Z No. of bitstreams: 1 2015 - Eriane de Lima Caminotto.pdf: 1339748 bytes, checksum: 753de2c181b04ca05748c103c2d6ec8b (MD5) / Made available in DSpace on 2017-01-24T16:23:42Z (GMT). No. of bitstreams: 1 2015 - Eriane de Lima Caminotto.pdf: 1339748 bytes, checksum: 753de2c181b04ca05748c103c2d6ec8b (MD5) Previous issue date: 2015-10-21 / Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do RJ - FAPERJ / Glaucoma is a disease that causes more blindness in dogs and has no cure, only treatment. The difficulty in the drainage of aqueous humor (AH) results in an increase in intraocular pressure (IOP), representing a significant risk factor for the occurrence of this condition, leading to irreversible damage to the progressive loss of visual field and vision as a whole death of retinal ganglion cells (RGC), loss of axons in the optic nerve and excavation of the same. In addition to this, several mechanisms may contribute to the development and progression of this disease, for example, unbalances of the renin-angiotensin-system (RAS), and cardiovascular and renal diseases. The measurement of IOP and the examination of the fundus are the methods used to diagnose this condition. The treatment of choice is based on eye drops that stimulate drainage of HA and / or decrease their production resulting in IOP control; however in many cases the treatment does not attain the desired effect, culminating in blindness. In an attempt to change this therapeutic perspective, this study compared, in healthy and normotensive Beagle dogs, systemic and ocular action of three drugs: timolol maleate 0.5% (non-selective beta-blocker used in cases of glaucoma) , captopril 0.1% and 0.5% (ECA1 inhibitor never before studied in greater concentration in dogs) and aceturato of diminazene - DIZE 0.1% and 0.5% (endogenous activator of ACE2, never before researched in eyes canines). The latter two drugs were formulated for ophthalmic use for the reduction of IOP, since it is known about the existence of SARS eye level, and that they have been successfully administered systemically at reducing systemic arterial pressure (SAP). Thus, before and after treatment, 18 dogs underwent the Schirmer Tear Test (TLS1) and blood collection for measuring the concentration of angiotensin-converting enzyme (ECA1) serum. For three consecutive days before treatment and at three times (6h, 12h and 18h) all animals have gone through four exams, always following the same order: evaluation of pupil size, IOP measurement, measurement of HR and SAP. After obtaining the normal range, the animals had the left eye subjected to predetermined protocols initially with 1 drop every 12 hours, 7 days a lower concentration and, in the other seven days, in the highest concentration. The adelfos eyes were control and every day in the three different times dogs went through the same initial exams. All drugs were good penetration and no adverse eye level. As for tear production, captopril is the most suitable for glaucoma patients and patients with keratoconjunctivitis sicca, while timolol is contraindicated for them. All decreased IOP values, and in the second week of treatment, with higher concentrations, reductions were more significant. The bradycardia was observed in captopril-treated animals 0.5%, with a reduction of almost 9% in HR compared to captopril in lower concentrations / O glaucoma ? uma das doen?as que mais causa cegueira em c?es e n?o possui cura, apenas tratamento. A dificuldade na drenagem do humor aquoso (HA) resulta no aumento da press?o intra-ocular (PIO) que representa um fator de risco significativo para a ocorr?ncia dessa afec??o, conduzindo ? danos irrevers?veis como a perda progressiva do campo visual e da vis?o como um todo, morte das c?lulas ganglionares da retina (CGR), perda de ax?nios do nervo ?ptico e escava??o do mesmo. Al?m deste, diversos mecanismos podem contribuir para o desenvolvimento e progress?o desta doen?a como, por exemplo, os desequil?brios do sistema-renina-angiotensina (SRA) e as doen?as cardiovasculares e renais. A mensura??o da PIO e o exame do fundo de olho s?o os m?todos mais utilizados para diagnosticar essa afec??o. O tratamento de escolha se baseia em col?rios que estimulam a drenagem do HA e/ou diminuam sua produ??o resultando no controle da PIO; por?m em muitos casos a terapia n?o atinge o efeito desejado e culmina em cegueira. Na tentativa de mudar essa perspectiva terap?utica, o presente trabalho comparou, em c?es sadios e normotensos da ra?a Beagle, a a??o sist?mica e ocular de tr?s f?rmacos: maleato de timolol 0,5% (beta-bloqueador n?o seletivo usado nos casos de glaucoma), captopril 0,1% e 0,5% (inibidor de ECA1 nunca antes estudado nesta maior concentra??o em c?es) e aceturato de diminazeno ? DIZE 0,1% e 0,5% (ativador end?geno de ECA2, nunca antes pesquisado em olhos caninos). Os dois ?ltimos f?rmacos foram formulados para uso oftalmol?gico visando a redu??o da PIO, uma vez que ? sabido sobre a exist?ncia do SRA a n?vel ocular e que os mesmos j? foram administrados sistemicamente com sucesso na redu??o press?o arterial sist?mica (PAS). Dessa forma, antes e ap?s os tratamentos, 18 c?es foram submetidos ao teste lacrimal de Schirmer (TLS1) e ? coleta sangu?nea para dosagem da concentra??o da enzima conversora de angiotensina (ECA 1) do soro.Durante tr?s dias consecutivos antes dos tratamentos e em tr?s hor?rios distintos (6h, 12h, e 18h) todos os animaispassaram por quatro exames, seguindo sempre a mesma ordem: avalia??o do di?metro pupilar, aferi??o da PIO, aferi??o da FC e da PAS.Ap?s a obten??o dos valores normais, os animais tiveram o olho esquerdo submetido aos protocolos pr?-determinados inicialmente com 1 gota, a cada 12 horas, por 7 dias na menor concentra??o e, nos demais 7 dias, na maior concentra??o. Os olhos adelfos foram o controle e, todos os dias nos tr?s hor?rios distintos os c?es passaram pelos mesmos exames iniciais. Todos os f?rmacos tiveram boa penetrabilidade e aus?ncia de efeitos adversos a n?vel ocular. Quanto ? produ??o lacrimal, o captopril ? o mais indicado para os pacientes glaucomatosos e portadores de ceratoconjuntivite seca, enquanto que o timolol ? contra-indicado para os mesmos. Todos diminu?ram os valores da PIO, sendo que na segunda semana de tratamento, com concentra??es maiores, as redu??es foram mais significativas. A bradicardia foi observada nos animais tratados com captopril 0,5%, com uma redu??o de quase 9% na FC, quando comparado com o captopril em menor concentra??o.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-25T15:40:39Z No. of bitstreams: 1 2015 - ?rica Cristina Xisto da Costa.pdf: 2608729 bytes, checksum: a906abcd8f725dc6509fedcf8de9e08c (MD5) / Made available in DSpace on 2017-01-25T15:40:39Z (GMT). No. of bitstreams: 1 2015 - ?rica Cristina Xisto da Costa.pdf: 2608729 bytes, checksum: a906abcd8f725dc6509fedcf8de9e08c (MD5) Previous issue date: 2015-07-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The gene encoding myostatin (MSTN), located on chromosome 18 (ECA 18) and the SNP BIEC2-808543 located in the intergenic region, which precedes the gene encoding the protein similar to the nuclear corepressor receptor dependent binder (LCORL), located on chromosome 3 (ACE 3) in horses, both positioned in regions that are associated with conformational traits of these animals. In view of this, we aimed to identify if the variations described in MSTN and LCORL loci exist in the study population; and to identify the effects of these polymorphisms on the growth profiles of these animals. For this purpose, the characteristics measured were weight, height at withers and hip height of foals at different ages, belonging to the Coudelaria e Campo de Instru??o de Rinc?o do Ex?rcito Brasileiro. Nonlinear mixed models were adjusted resulting from the combination of six nonlinear simple models, Brody (1945), Gompertz (Winsor, 1932), Logistics (Ratkowski, 1983), Von Bertalanffy (1957), generalized Michaelis- Menten (Lopez et al., 2000) and Richards (1959) associated with four types of variance functions for each model, homogeneous, exponential, asymptotic and staggered. The polymorphism described in the promoter region of the MSTN gene was not found in the studied population, in which there has been only the T allele, however the BIEC2-808543 polymorphism, located in the region prior to the LCORL gene is significantly associated (P <0, 05) to the characteristics evaluated, in which the animals who presented the genotype TT were smaller and lighter when compared to the other genotypes. There was no significant difference between animals with CT and CC genotype. The model that best describes the growth curve for body mass variance is the model of Brody (1945) associated with the scaled variance for the variable height at the withers the model that best fit was the Von Bertalanffy (1957) (adjusted without polymorphism effect in b) parameter associated with the asymptotic variance and the characteristic hip height the model that best described was that of Brody (1945) associated with the asymptotic variance, explaining that the nonlinear mixed models are indeed promising to describe equine growth curves, for the simple models did not differ much among themselves what defined in fact the selection of the model was the variance, being for body mass staggered variance and the height at the withers and on the back, the asymptotic variance. This polymorphism can be used as molecular markers for early selection of foals as to the characteristics evaluated / O gene que codifica a miostatina (MSTN), localizado no cromossomo 18 (ECA 18) e o SNP BIEC2-808543 localizado na regi?o interg?nica que antecede o gene que codifica a prote?na semelhante a correpressor de receptor nuclear dependente de ligante (LCORL), localizado no cromossomo 3 (ECA 3) de cavalos, ambos posicionados em regi?es que est?o associadas ?s caracter?sticas conformacionais destes animais. Diante disto, objetivamos identificar se as varia??es descritas nos loci MSTN e LCORL, existem na popula??o em estudo; al?m de verificar os efeitos desses polimorfismos sobre os perfis de crescimento desses animais. Com este intuito foram mensuradas as caracter?sticas massa corporal, altura na cernelha e altura na garupa de potros em diversas faixas et?rias, pertencentes ? Coudelaria e Campo de Instru??o de Rinc?o do Ex?rcito Brasileiro. Foram ajustados modelos n?o lineares mistos que resultaram da combina??o de seis modelos n?o lineares simples, Brody (1945), Gompertz (Winsor, 1932), Log?stico (Ratkowski, 1983), Von Bertalanffy (1957), Michaelis-Menten generalizado (L?pez et al., 2000) e Richards (1959), associados a quatro tipos de fun??es de vari?ncia para cada modelo, homog?nea, exponencial, assint?tica e escalonada. O polimorfismo descrito na regi?o promotora do gene MSTN n?o foi encontrado na popula??o estudada, na qual observa-se apenas o alelo T, entretanto o polimorfismo BIEC2-808543, localizado na regi?o que antecede o gene LCORL, est? significativamente associado (P<0,05) ?s caracter?sticas avaliadas, sendo os animais que apresentaram o gen?tipo TT menores e mais leves quando comparado com os demais gen?tipos. N?o foi observada diferen?a significativa entre os animais com gen?tipo TC e CC. O modelo que melhor descreve a curva de crescimento para a vari?vel massa corporal ? o modelo de Brody (1945) associado com a vari?ncia escalonada, para a vari?vel altura na cernelha o modelo que melhor se ajustou foi o de Von Bertalanffy (1957) (ajustado sem efeito de polimorfismo no par?metro b) associado com a vari?ncia assint?tica e para a caracter?stica altura na garupa o modelo que melhor a descreveu foi o de Brody (1945) associado ? vari?ncia assint?tica, elucidando que os modelos n?o-lineares mistos s?o de fato promissores para a descri??o de curvas de crescimento de equinos, pois os modelos simples n?o diferiram muito entre si o que definiu de fato a sele??o do modelo foi a vari?ncia, sendo para massa corporal a vari?ncia escalonada e para as alturas, na cernelha e na garupa, a vari?ncia assint?tica. Este polimorfismo pode ser utilizado como marcador molecular para sele??o precoce de potros quanto ?s caracter?sticas avaliadas

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-26T10:49:56Z No. of bitstreams: 1 2015 - Rafaela Scalise Xavier de Freitas.pdf: 1344462 bytes, checksum: 1e3669fc0b4da746d7a41a31525f3492 (MD5) / Made available in DSpace on 2017-01-26T10:49:56Z (GMT). No. of bitstreams: 1 2015 - Rafaela Scalise Xavier de Freitas.pdf: 1344462 bytes, checksum: 1e3669fc0b4da746d7a41a31525f3492 (MD5) Previous issue date: 2015-06-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This survey was divided into two chapters, the first one producing and characterizing Urochloa humidicola methanol extract in order to present secondary metabolites classes and bromathological and chemical composition. These metabolites have several functions within the plant and are associated to defense system, protecting the environment where they have been living. These compounds have been used on animal feed in the reason presenting antimicrobial properties that could be employed for inducing ruminal fermentation. Phytochemical screening tests and chemical composition of U. humidicola methanol extract and in natura plant were carried out on this present survey. The following secondary compounds classes: saponins, tannins, flavonoids, non-protein amino acids, cardiotive glycosides, steroids, tripernoids, catechins and saccharides were indentified U. humidicola methanol extract in regarding to in natura plant showed 10,20% and 5,17% crude protein concentrations, 35% and 1,51% lipids and 9,59% aand 39,92% non-fibrous carbohydrates, respectively. These results might be explained by percolation with methanol extraction methods extracting only soluble constituents transporting silted protein, lipids and ash to the extract. The second chapter of this survey aimed evaluating U. humidicola extract addition effect containing saponin associated to U. brizantha assessing gases production, (methane and carbon dioxide), ruminal kinetics, dry matter degradation and short chain fatty acids production (SCFA: acetate, propionate and butyrate), as well. Plant extracts have been an alternative inducing ruminal fermentation by secondary metabolites in the reason they are from natural sources and with no residue hazards in products like meat and milk. Ruminal fermentation induction could reduce methane production, as well as, increase acetate: propionate ratio and improve food degradation. Four U. humidicola methanol extract concentrations (0, 75, 150 and 250 g/L) on U. brizantha degradability by in vitro gases production were tested. At 150 g/L extract concentration gas production from fibrous carbohydrates was 118,21 mL. However, the highest fiber concentration rate occurred at 150 g/L. Increasing extract concentrations (75, 150 and 250 g/L) soluble fraction values were: 10,27; 7,46 and 14,07%, respectively. Effective ruminal degradability at 75, 150 and 250 g/L concentrations for passage rates for an animal in maintenance were 38,53%, 27,71% and 20,30%, respectively. Extract concentrations increase exerted a linear effect (P<0.05) on ruminal pH values being more evident at high extract concentration (250 g/L) as 5,73 and 5,43 at 12 and 24 hs, respectively. CO2 averages in regarding to incubated and degraded dry matter did not differ with extract concentrations increase at 12 hs. Methane averages in regarding to incubated and degraded dry matter were no significative by regression analysis. Treatment at 250 g/L concentration presented the lowest value for methane at 12 hs. At 75 g/L concentration, total SCFA (acetic, propionic and butyric acid) increase at 12 and 24 hs was reported. U. humidicola methanol extract different concentration addition improved U. brizantha fermentation kinects parameters at 150 g/L and 250 g/L concentrations. However, negative effect on U. brizantha dry matter degradation and ruminal pH values according to extract concentrations increase was reported. Strong correlation between pH values and dry matter degradation (p=0,61, P<0,05) was presented. Carbon dioxide concentration increased, as well as, methane production decreased U. humidicola crude methanol extract presented potential for use as ruminal fermentation promoter. New studies about U. humidicola extract employing animals for justifying its efficiency as food additive should, furthermore, be developed. / Este trabalho foi dividido em dois cap?tulos, o primeiro teve por objetivo produzir e caracterizar o extrato metan?lico de Urochloa humidicola, com o intuito de conhecer as classes de metab?litos secund?rios presentes e a composi??o qu?mico-bromatol?gica. Estes metab?litos possuem diversas fun??es dentro dos vegetais e est?o associados ao sistema de defesa, os protegendo no ambiente que vivem. Estes compostos est?o sendo utilizados na alimenta??o animal por apresentarem propriedades antimicrobianas que podem ser empregadas para induzir a fermenta??o ruminal. Para este estudo foram realizados os testes de prospec??o fitoqu?mica e as an?lises de composi??o bromatol?gica do extrato metan?lico de U. humidicola e da U. humidicola in natura. Foram identificadas as seguintes classes de compostos secund?rios: saponinas, taninos, flavonoides, amino?cidos n?o proteicos, glicos?deos cardioativos, ester?ides e tripern?ides, catequinas e sacar?deos. O extrato metan?lico de U. humidicola em rela??o ? planta in natura, apresentaram concentra??es de prote?na bruta de 10,20% e 5,17%, e mat?ria mineral de 16,14% e 8,14%, extrato et?reo de 35% e 1,51%, carboidrato n?o fibroso, 9,59% e 39,92%, respectivamente. Esse resultado pode ser explicado pelo m?todo de extra??o que foi por percola??o com metanol, extraindo somente os constituintes sol?veis carreando somente prote?na, lip?deos e cinzas para o extrato. No segundo cap?tulo deste trabalho teve como objetivo avaliar o efeito da adi??o de extrato de U. humidicola contendo saponina, associada ? Urochloa brizantha, avaliando a produ??o de gases (metano e de di?xido de carbono), a cin?tica ruminal, a degrada??o da mat?ria seca e produ??o de ?cidos graxos de cadeia curta (AGCC; acetato, propionato e butirato). Os extratos vegetais de plantas s?o uma alternativa para induzir da fermenta??o ruminal por possu?rem metab?litos secund?rios, por serem de fontes naturais e sem riscos de res?duos nos produtos como carne e leite. A indu??o da fermenta??o ruminal pode reduzir a produ??o de metano, aumentar a rela??o de acetato: propionato e melhorar a degrada??o do alimento. Foram testados quatro concentra??es de extrato metan?lico de U. humidicola (0, 75, 150 e 250 g/L) sobre a degradabilidade da U. brizantha pela produ??o de gases in vitro. Na concentra??o de 150 g/L do extrato, a produ??o de g?s proveniente dos carboidratos fibrosos, foi de 118,21 mL. No entanto, a maior taxa de degrada??o dos carboidratos fibrosos ocorreu na concentra??o 150 g/L. Com o aumento das concentra??es de extrato (75, 150 e 250 g/L) os valores da fra??o sol?vel foram de 10,27; 7,46 e 14,07% respectivamente. A degradabilidade ruminal efetiva nas concentra??es de (75, 150 e 250 g/L) para as taxas de passagem para um animal em manten?a foram de 38,53%, 27,71% e 20,30%, respectivamente. O aumento das concentra??es de extrato exerceu um efeito linear (P<0,05) sobre os valores de pH ruminal, sendo mais evidente na alta concentra??o de extrato (250 g/L) que foi de 5,73 e 5,43 nos tempos de 12 e 24 horas, respectivamente. As m?dias de CO2 com rela??o ? mat?ria seca incubada e degradada n?o diferiram entre si com o aumento das concentra??es de extrato nos tempos de 12 horas. As m?dias de metano com base na mat?ria seca incubada e degradada n?o apresentaram signific?ncia para an?lise de regress?o. O tratamento com a concentra??o de 250 g/L de extrato apresentou menor valor para metano no tempo de 12 horas. A concentra??o de extrato (75 g/L) proporcionou um aumento do total de AGCC, ?cido ac?tico, ?cido propi?nico e ?cido but?rico tanto no tempo de 12 e 24 horas. A adi??o das diferentes concentra??es de extrato metan?lico de U. humidicola melhorou os par?metros da cin?tica da fermenta??o da U. brizantha nas concentra??es de 150 e 250 g/L. Mas causou um efeito negativo sobre a degrada??o da mat?ria seca da U. brizantha e no pH ruminal com o aumento das concentra??es de extrato. Existe uma forte correla??o entre os valores de pH e degrada??o da mat?ria seca (?=0,61, P<0,05). Aumentou as concentra??es de g?s carb?nico e reduziu a produ??o de metano. O extrato metan?lico bruto de U. humidicola tem potencial para uso como indutor da fermenta??o ruminal. ? necess?rio ? purifica??o e o isolamento da saponina do extrato para comprovar o efeito ben?fico sobre a fermenta??o ruminal. S?o imprescind?veis novos estudos com o extrato de U. humidicola, utilizando animais para se comprovar a efici?ncia na utiliza??o como aditivo alimentar

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-26T14:34:24Z No. of bitstreams: 1 2016 - Danielle Affonso Sampaio.pdf: 3006939 bytes, checksum: 839c5d77a13fd99ad3c749c33dc2530a (MD5) / Made available in DSpace on 2017-01-26T14:34:25Z (GMT). No. of bitstreams: 1 2016 - Danielle Affonso Sampaio.pdf: 3006939 bytes, checksum: 839c5d77a13fd99ad3c749c33dc2530a (MD5) Previous issue date: 2016-05-19 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq / Seed integument plays an important role in the plant life cycle, monitoring the embryo development and germination. Informations about cell structure and physico-chemical characteristics of the Araucaria angustifolia seed coat are important to its correct functional interpretation. Thus, the aim of this study was the anatomical and physico-chemical characterization of the Araucaria angustifolia seed integument. Anatomical features were observed using different microscopy techniques (brightfield microscopy, fluorescence microscopy and scanning electron microscopy) and histochemical tests (Lugol, Wiesner, Sudan IV and potassium dichromate ? K2Cr2O7). Chemical analysis included the extractive, holocelulose and Klason lignin contents (untreated and treated with sodium hydroxide ? NaOH). Functional groups of the integument layers and lignin were observed by Fourier transformed infrared spectroscopy using a VARIAN 640-IR FT-IR spectrometer. Colour analyses were performed in CIE 1976 L*a*b* space colour according to ISO 11664-4:2008 standard using CM 2600d spectrometer. Wettability of the layers was evaluated by contact angle analysis with the drop shape analyser DSA 100. The Araucaria angustifolia seed integument is composed by three distinct layers: exotesta, mesotesta and endotesta. The layers have different chemical and anatomical characteristics. Lignin structure of the integument layers was classified as guayacil (G) type. The endotesta was the layer with higher chromaticity due to higher extractive content and phenolic substances. The wettability varied between layers according to ther strusture. The results of this study contribute to a better understanding of the functioning of the Araucaria angustifolia seed integument and corroborate to future studies on seed physiology / O tegumento de semente desempenha um papel importante no ciclo de vida do vegetal, controlando o desenvolvimento do embri?o e a germina??o. O conhecimento da estrutura celular e das caracter?sticas f?sico-qu?micas do tegumento da semente de Araucaria angustifolia ? importante para a correta interpreta??o funcional do mesmo. Sendo assim, o objetivo deste estudo consistiu na caracteriza??o anat?mica e f?sico-qu?mica do tegumento da semente de Araucaria angustifolia. As caracter?sticas anat?micas foram observadas atrav?s de diferentes t?cnicas de microscopia (microscopia de campo claro, microscopia de fluoresc?ncia e microscopia eletr?nica de varredura) e testes histoqu?micos (Lugol, Wiesner, Sudan IV e dicromato de pot?ssio ? K2Cr2O7). Nas an?lises qu?micas, determinou-se os teores de extrativos, holocelulose e lignina de Klason tratada e n?o-tratada com hidr?xido de s?dio ?NaOH. Os grupos funcionais das camadas do tegumento e da lignina foram observados por espectroscopia no infravermelho por transformada de Fourier atrav?s do espectr?metro VARIAN 640-IR FT-IR. As an?lises colorim?tricas foram realizadas no espa?o de cor L*a*b* CIE 1976 segundo a norma ISO 11664-4: 2008 utilizando o espectrofot?metro CM 2600d. A molhabilidade das camadas foi avaliada atrav?s de an?lises de ?ngulo de contato com o sistema de formato da gota DSA 100. O tegumento da semente de Araucaria angustifolia ? composto por tr?s camadas distintas: exotesta, mesotesta e endotesta. As camadas apresentam caracter?sticas qu?micas e anat?micas distintas. A estrutura da lignina das camadas do tegumento foi classificada como guaiac?lica (G). A endotesta foi a camada com maior cromaticidade devido ao maior teor de extrativos e subst?ncias fen?licas. A molhabilidade variou entre as camadas em fun??o da sua estrutura. Os resultados deste estudo contribuem para uma melhor compreens?o do funcionamento do tegumento da semente de Araucaria angustifolia, podendo corroborar com estudos futuros sobre a fisiologia da semente

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-02-14T12:00:15Z No. of bitstreams: 1 2016 - Gabriela Mastrangelo Gon?alves.pdf: 1218648 bytes, checksum: d31754b24278f68ec75382b5ff3932c0 (MD5) / Made available in DSpace on 2017-02-14T12:00:15Z (GMT). No. of bitstreams: 1 2016 - Gabriela Mastrangelo Gon?alves.pdf: 1218648 bytes, checksum: d31754b24278f68ec75382b5ff3932c0 (MD5) Previous issue date: 2016-02-23 / Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do RJ - FAPERJ / Several drugs in current use were discovered during experimental tests and by observing animals. When a new compound looks promising, it usually undergoes changes in its chemical structure in order to perfect its selectivity, potency and therapeutic efficacy. The aim of this study was to evaluate the antinociceptive and anti-inflammatory activities of a new synthetic hybrid compound (?)-4-chloro-6-(naphthalen-1-yl)-tetrahydro-2H-pyran-2-yl-methanol (CTHP) prepared from a previous prototype acid, (?) - cis- (6-ethyl-tetrahydropyran-2-yl) Formic. The compound CTHP was evaluated in acute pain induction assays. Oral administration of the compound was able to induce antinociceptive activity in models of writhing induced by acetic acid, formalin (both stages) and tail flick. To elucidate the mechanism of action of the compound, the tail flick model was used. This model was perform by prior administration of naloxone (opioid antagonist non-selective), where we observed the inhibition of the effect produced by the compound. The selective involvement of opioid receptors (?, ? and ?) was then evaluated by prior administration of methylnaltrexone, naltrindol, and nor-binaltorphimine, respectively, where only nor-binaltorphimine was able to reduce the analgesic effect of the compound. To evaluate the possible role of the NO/cGMP/KATP, animals were pretreated with N-nitro-L-arginine methyl ester (L-NAME), 1H- [1,2,4 ] oxadiazolo [4,3-a] quinoxalin-1-one (ODQ) (inhibitor of guanylate cyclase sensitive to nitric oxide), and glibenclamide (blocker of the ATP-regulated potassium channels), where reduction was observed with the administration of analgesic effect prior to all of these. In the tolerance induction test, both morphine and compound developed tolerance, however the compound perform at a slower rate and developed cross-tolerance with morphine. To assess the involvement of serotonin pathway in the activity of the compound, daily administration for 3 days of 4-chloro-DL-phenylalanine (inhibitor of the enzyme tryptophan hydroxylase) was performed. No changes in the analgesic effect of the compound was noted, with regard to the involvement of serotonin pathway. The open field model was used to assess the possibility of interference from motor performance on the analgesic effect, which demonstrated absence of this interference. As for anti-inflammatory activity results in paw edema test indicate anti-oedematogenic effect of compound. There was a decrease in the number of total leukocytes, indicating that the compound was able to reduce existing inflammation in leukocyte migration in the air pouch model. The compound also demonstrated an inhibitory activity on TNF-? production and selective inhibition of COX-2 enzyme. These results indicate significant antinociceptive activity of the compound without evidence of motor impairment. The compound CTHP showed central analgesic effect, which has contribution of opioid systems (selective for the ?-like receptors) and nitrergic in its mechanism of action. It has also showed an anti-inflammatory activity, with inhibition of leukocyte migration, TNF-? production and selective inhibitory activity on COX-2. / Diversos f?rmacos de uso corrente foram descobertos durante ensaios experimentais e mediante a observa??o em animais. Quando um novo composto parece promissor, geralmente este sofre altera??es em sua estrutura qu?mica a fim de aperfei?oar a sua seletividade, pot?ncia e efic?cia terap?utica. O objetivo deste estudo foi avaliar as atividades antinociceptiva e anti-inflamat?ria de um novo composto sint?tico (?)-4-cloro-6-(naftaleno-1-il)-tetrahidro-2h-pirano-2-il-metanol (CTHP) preparado a partir de um prot?tipo anterior, ?cido (?)-cis-(6-etil-tetrahidropirano-2-il) f?rmico. O composto CTHP foi avaliado em ensaios de indu??o de dor aguda. A administra??o oral do composto foi capaz de induzir atividade antinociceptiva nos modelos de contor??es abdominais induzidas por ?cido ac?tico, formalina (em ambas as fases) e retirada da cauda. Para elucida??o do mecanismo de a??o do composto, o modelo de retirada de cauda foi utilizado. Neste modelo foi realizada a administra??o pr?via de naloxona (antagonista opioide n?o-seletivo), em que foi observada a inibi??o do efeito produzido pelo composto. Assim, foi ent?o avaliada a participa??o seletiva de receptores opioides (?, ? e ?), atrav?s de administra??o pr?via de metilnaltrexona, naltrindol e nor-binaltorfimina, respectivamente, onde somente a nor-binaltorfimina foi capaz de reduzir o efeito antinociceptivo do composto. Para avaliar a poss?vel participa??o da via NO/GMPc/KATP, os animais foram pr?-tratados com N-nitro-arginina-L-metil ?ster (L-NAME), 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalina-1-ona (ODQ) (inibidor da guanilato ciclase sens?vel ao ?xido n?trico) e glibenclamida (bloqueador de canais de pot?ssio regulados por ATP), foi observado redu??o do efeito antinociceptivo com a administra??o pr?via de todos estes. No teste de indu??o de toler?ncia, tanto a morfina quanto o composto desenvolveram toler?ncia, por?m o composto de forma mais lenta e houve desenvolvimento de toler?ncia cruzada com a morfina. Para avaliar o envolvimento da via serotonin?rgica na atividade do composto, foi realizada a administra??o di?ria por 3 dias de 4-cloro-DL-fenilalanina (inibidor da enzima triptofano hidroxilase). Nenhuma altera??o no efeito antinociceptivo do composto foi observado, no que diz respeito ao envolvimento da via serotonin?rgica. J? o modelo de campo aberto foi utilizado para avaliar a possibilidade de interfer?ncia da performance motora sobre o efeito antinociceptivo, foi demonstrada aus?ncia desta interfer?ncia. Quanto ? atividade anti-inflamat?ria, o resultado no teste de edema de pata indica efeito antiedematog?nico do composto. Houve uma diminui??o na quantidade de leuc?citos totais, indicando que o composto foi capaz de reduzir a migra??o leucocit?ria na inflama??o existente na bolsa de ar subcut?neo. O composto tamb?m demonstrou atividade inibit?ria sobre a produ??o de TNF-? e inibi??o seletiva da enzima COX-2. Esses resultados indicam atividade antinociceptiva significativa do composto, sem evid?ncias de comprometimento motor. O composto CTHP demonstrou efeito antinociceptivo central, tendo este ?ltimo contribui??o dos sistemas opioide (seletivo para receptores do tipo ?) e nitr?rgico em seu mecanismo de a??o. E ainda, atividade anti-inflamat?ria, com inibi??o da migra??o leucocit?ria, de TNF-? e atividade inibit?ria seletiva sobre COX-2.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-02-14T13:37:41Z No. of bitstreams: 1 2016 - Erick de Oliveira Santos Costa.pdf: 678050 bytes, checksum: 440f614342d47250b079c0e29a8e02d2 (MD5) / Made available in DSpace on 2017-02-14T13:37:41Z (GMT). No. of bitstreams: 1 2016 - Erick de Oliveira Santos Costa.pdf: 678050 bytes, checksum: 440f614342d47250b079c0e29a8e02d2 (MD5) Previous issue date: 2016-03-31 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / One of the goals of this work is to present and analyze the difficulty of thinking about the nature and the movement facing to the notion of becoming. In fact, it seems that the necessity of matching the notions of being and non-being give nature a contradictory core or a sign of indeterminacy. We will see therefore how Aristotle deals with this dilemma. We understand that the philosopher of Stagira points out the need to conceive the nature and movement as a phenomenon to dissolve the dilemmas are present in previous philosophy / Um dos objetivos do presente trabalho ? expor e analisar a dificuldade de se pensar a natureza e o movimento frente ? no??o de devir. Com efeito, parece que a necessidade de se conciliar as no??es de ser e n?o-ser conferem a natureza um ?mago contradit?rio ou um ?ndice de indetermina??o. Veremos, por conseguinte, como Arist?teles lida com esse dilema. Entendemos que o fil?sofo de Estagira aponta para a necessidade de conceber a natureza e o movimento enquanto fen?meno para dissolver os dilemas presentes na filosofia precedente

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2017-02-15T12:03:41Z No. of bitstreams: 1 Viviane-Silva-Ramos.pdf: 1273367 bytes, checksum: fa39fb3f9fb99d110b14407139946745 (MD5) / Made available in DSpace on 2017-02-15T12:03:41Z (GMT). No. of bitstreams: 1 Viviane-Silva-Ramos.pdf: 1273367 bytes, checksum: fa39fb3f9fb99d110b14407139946745 (MD5) Previous issue date: 2011-03-02 / This work started from the perception that the Federal Institutes of Education, Science and Technology, created on December 29th, 2008, through the law number 11.892, have hard duties and challenge responsibilities concerning the Brazilian society. For this reason, we intended to analyze the role of these Federal Institutes, looking for the aims, characteristics, and drawn goals to this new institutional model, mainly regarding the themes about the local development and the territory, very discussed in the law. In face of that, we took as example to this study the school called Campus Sert?o, from the Federal Institute of Education, Science and Technology of Rio Grande do Sul in order to verify if the educational activities are being done according to the referred law. With this in mind, the research was applied by the qualitative evaluation, with the exploratory research, through the case study, and the bibliographic research as well. A brief overview about the following topics was necessary: the history of the professional and technological education in Brazil; the Federal Network of Professional, Scientific and Technological Education as well as its spreading out; of how the Federal Institutes were constituted and, besides that, the history of Campus Sert?o since its foundation. The concepts of territory and local development were also studied through several authors who carried about them, getting a better understanding within the law context. The results were presented and discussed all the way through the thinking about the articles and subsections from the Law Foundation of the Federal Institutes that deal, explicit or implicitly, with the commitment of these institutions to the socioeconomic and cultural local improvement in their enclosure area. In addition to that, this study was worried about how the teaching, the research and the extension worked out in the Campus Sert?o before its incorporation to the Federal Institute of Rio Grande do Sul and what has changed during the two years after it became an Institute. Finally, a comparative analysis was shown between the achievements by the Campus and what is still considered necessary to be done in its working method in order to reach the law demands and improve its essential role to the local and bounded growth. / O presente estudo teve sua origem a partir da constata??o de que os Institutos Federais de Educa??o, Ci?ncia e Tecnologia, criados em 29 de dezembro de 2008, atrav?s da lei n? 11.892, t?m atribui??es ousadas e responsabilidades desafiadoras para com a sociedade brasileira. Por isso, se prop?s analisar o papel desses Institutos Federais, levando em considera??o as finalidades, caracter?sticas e objetivos tra?ados para esse novo modelo institucional, especialmente no que tange ?s tem?ticas do desenvolvimento local e do territ?rio, muito enfatizadas no texto legal. Diante disso, tomou-se como exemplo o caso do Campus Sert?o, do Instituto Federal de Educa??o, Ci?ncia e Tecnologia do Rio Grande do Sul para verificar se suas atividades educativas v?m sendo realizadas em conson?ncia com o que preconiza a referida lei. Para a consecu??o dos objetivos propostos, a pesquisa foi aplicada e utilizou a abordagem qualitativa, com car?ter explorat?rio, atrav?s da pesquisa bibliogr?fica e do estudo de caso. Foi realizado um breve resgate hist?rico da educa??o profissional e tecnol?gica no Brasil, da Rede Federal de Educa??o Profissional, Cient?fica e Tecnol?gica e sua expans?o, de como se deu a constitui??o dos Institutos Federais e, ainda, da trajet?ria do Campus Sert?o desde a sua cria??o. Os conceitos de territ?rio e desenvolvimento local tamb?m foram estudados sob a ?tica de v?rios autores que tratam desses temas, para maior entendimento no contexto dos dispositivos legais. Os resultados foram apresentados e discutidos atrav?s da reflex?o sobre os artigos e incisos da lei de cria??o dos Institutos Federais que tratam, expl?cita ou implicitamente, da obriga??o que estas institui??es t?m com o desenvolvimento socioecon?mico e cultural local no seu territ?rio de abrang?ncia. Al?m disso, foi abordado como o ensino, a pesquisa aplicada e a extens?o vinham sendo desenvolvidos no Campus Sert?o antes de sua integra??o ao Instituto Federal do Rio Grande do Sul e o que mudou nos dois anos que sucederam a implanta??o da nova institucionalidade. Encerrou-se com uma an?lise comparativa entre o que j? vem sendo realizado pelo Campus e o que ainda ? preciso ser melhor adequado no seu funcionamento a fim de atender plenamente ?s exig?ncias legais e aprimorar sua atua??o na promo??o do desenvolvimento local e regional.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-02-15T15:46:57Z No. of bitstreams: 1 2015 - Ana?s Gabriela Villarreal Laguna.pdf: 1531103 bytes, checksum: 8567bc787b67e1d16f71708035dbfc97 (MD5) / Made available in DSpace on 2017-02-15T15:46:58Z (GMT). No. of bitstreams: 1 2015 - Ana?s Gabriela Villarreal Laguna.pdf: 1531103 bytes, checksum: 8567bc787b67e1d16f71708035dbfc97 (MD5) Previous issue date: 2015-12-21 / Otitis is defined as an inflammation, acute or chronic, of the ear canal and it?s considered one of the most common diseases in small animal practice. It possesses various perpetuating agents, bacteria and yeast being the most common. Treatment is based in the recognition of the primary causes and predisposing factors, and the resolution of secondary infections. A greater sensitivity in feline ear canal has also been shown, when compared to dogs. With this in mind, a product with the least possible active ingredients would be the ideal topical treatment in felines. However, the majority of available products combine antibiotics and antifungals, this combination not being necessary in all cases; for bacterial otitis, very few specific products exist, and some of them present a proven ototoxic potential. In face of this problem, the efficacy of a new formulation using levofloxacin for the specific treatment of bacterial otitis was made by the Farmacologia e Farmacometria sector of the LQEPV ? UFRRJ, using isopropylic alcohol as vehicle. Eighteen adult felines, belonging to the Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria (LQEPV-UFRRJ), diagnosed with bacterial otitis according to clinical signs (erythema, inflammation, pruritus, hyperpigmentation and secretions), as well as cytological exams and video-otoscopy, were chosen. Individual data was registered in specific formularies for comparison of pretreatment and post treatments evaluations. After the initial evaluation the animals were divided into three groups, according to treatment: in Group A or control (6 animals) a placebo (vehicle) was used; in Group B (6 animals) a formulation of levofloxacin 0, 5% was used; Group C (6 animals) a formulation of de levofloxacin 0, 5% + dexamethasone 0, 02% was used. In every case 0, 5 ml was applied to each ear, once a day for 15 consecutive days. Post treatment video-otoscopies were performed on day +21, and post treatment cytologies were performed on days +7, +14 and +21. The animals were always observed by the same evaluator, avoiding errors of concordance and checking for possible reactions to the product. None of the animals presented adverse reactions to the product during the treatment period, besides slight discomfort immediately after application. Animals from Group A continued with the same scores until the end of the experiment. Animals from Group B presented an initial clinical improvement, but some of them developed a fungal otitis as time passed. Animals from Group C presented a significant improvement since day +7, with no secondary alterations through time. It is demonstrated that a formulation of de levofloxacin + dexamethasone can be efficient and safe for the treatment of feline bacterial otitis / A otite ? definida como uma inflama??o, aguda ou cr?nica, do conduto auditivo e ? uma das doen?as mais frequentes na cl?nica de animais de companhia. Possui diversos agentes perpetuantes, sendo mais comuns as bact?rias e leveduras. O tratamento e baseado no reconhecimento de causas principais e fatores predisponentes, assim como a resolu??o de infec??es secund?rias. Tem sido relata uma sensibilidade maior nos condutos auditivos dos gatos, quando comparados com c?es. Deste modo, um produto com poucos ingredientes farmacologicamente ativos seria o tratamento t?pico ideal em felinos. No entanto, a maioria dos produtos para otite dispon?veis no mercado combina tanto antibi?ticos, como antif?ngicos, n?o sendo esta combina??o necess?ria em todos os casos; especificamente para otites bacterianas, existem poucos produtos espec?ficos, e muitos apresentam um potencial otot?xico j? comprovado. Perante este problema, foi avaliada a efic?cia de uma nova formula??o a utilizando levofloxacina para o tratamento espec?fico da otite bacteriana em felinos, o produto foi confeccionado pelo setor de Farmacologia e Farmacometria do LQEPV ? UFRRJ, utilizando como ve?culo ?lcool isoprop?lico. Foram selecionados 18 felinos adultos pertencentes ao Laborat?rio de Quimioterapia Experimental em Parasitologia Veterin?ria (LQEPV-UFRRJ), diagnosticados com otite bacteriana de acordo com sinais cl?nicos (eritema, inflama??o, prurido, hiperpigmenta??o e presen?a de secre??o), exame citol?gico e v?deo-otoscopia. Os dados individuais foram registrados em formul?rios espec?ficos para compara??o das avalia??es inicias e p?s-tratamento. Ap?s as avalia??es iniciais, os animais foram divididos em tr?s grupos experimentais: no grupo A, controle, seis animais utilizaram um Placebo (ve?culo); no grupo B, seis animais utilizaram a formula??o contendo Levofloxacina a 0,5%; e no grupo C, seis animais foram medicados com a associa??o de Levofloxacina 0,5% com dexametasona 0,02%. Em todos os casos foram instilados 0,5ml em ambas as orelhas uma vez por dia, durante 15 dias consecutivos. Exames vide-otosc?picos p?s-tratamento foram realizadas no dia +21, e citologias nos dias +7, +14 e +21. Os animais foram observados diariamente sempre pelo mesmo avaliador, evitando assim erros de concord?ncia e acompanhando poss?veis rea??es adversas ao produto. Nenhum animal apresentou rea??o adversa ao produto durante o per?odo de tratamento, al?m de incomodo no momento imediato ap?s a aplica??o. Os animais do grupo A continuaram com o mesmo score registrado no dia 0 at? finalizado o experimento. Os animais do grupo B apresentaram melhoria inicial, porem alguns deles desenvolveram otite f?ngica ao decorrer do tempo. J? os animais do grupo C apresentaram uma melhora significativa desde o dia +7, sem altera??es secund?rias no decorrer do experimento. Demostra-se assim que a associa??o de levofloxacina 0,5% com dexametasona 0,02% pode ser eficaz e segura para o tratamento da otite bacteriana em felinos

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-02-15T16:34:59Z No. of bitstreams: 1 2016 - Vitor dos Santos Almeida.pdf: 2652432 bytes, checksum: 7f27ae8d8b345f1dc55ee863302bfda7 (MD5) / Made available in DSpace on 2017-02-15T16:34:59Z (GMT). No. of bitstreams: 1 2016 - Vitor dos Santos Almeida.pdf: 2652432 bytes, checksum: 7f27ae8d8b345f1dc55ee863302bfda7 (MD5) Previous issue date: 2016-04-15 / The analysis of mixtures is a common place challenge in the laboratory. Conventional methods demand a previous treatment which may be time consuming. At present, Nuclear Magnetic Resonance may be employed for the analysis of mixtures by using the DOSY (diffusion ordered spectroscopy) technique, whereby the different components of a mixture are separated by their diffusion coefficients. Pitches, in general, are products which are employed as starting material for the production of a variety of carbon materials, such as anodes for the production of aluminum, graphite electrodes for the steel industry, and carbon fibers. Pitches obtained from coal tar, itself a residue in the production of coke, are the most traditionally used. However, it is a carcinogen and therefore has had its use greatly diminished. Petroleum pitches, which are produced from decanted oil, may be a safer and less expensive alternative. The decanted oil is a heavy fraction obtained from catalytic cracking of petroleum. The difficulties in controlling its processing are derived from its complex composition, which includes paraffins, aromatic and heteroaromatic compounds. The present work investigates the analysis by NMR-DOSY of a mixture of model compounds present in decanted oil. Thus, a mixture of Tetracosane, Phenanthrene, 9-Mehylanthracene, Pyrene, Carbazole, 9-metil Carbazol, Dibenzothiophene, Dibenzofuran, Hexadecane, 1-Methylnaphthalene, Thiophene, Tetrahydrofuran was analyzed by NMR with an Avance III 500 MHz NMR spectrometer equipped with a BBO probe with a gradient on z-axis, at 20?C. To help in the separation by diffusion, the following polymers were used: poly-(ethyleneglycol), poly-(vinylpyrrolidone), poly-(dimethylsiloxan), poly-(butylmethacrylate) e poly-(vinylacetate).The shift reagent Eu(fod)3 was also employed. The results from DOSY-INEPT experiments show that poly-(butylmethacrylate) is the matrix which afforded the best separation of the different components of the mixture, followed by poly-(vinylacetate), poly-(ethyleneglycol) and finally, poly-(vinylpyrrolidone), which showed satisfactory results / Um grande desafio, muitas das vezes at? comum nos laborat?rios, ? a an?lise de misturas. Os m?todos convencionais em sua maioria exigem um tratamento pr?vio das misturas, o que demanda tempo, al?m de interferir diretamente no equil?brio do sistema. Hoje em dia, a t?cnica de Resson?ncia Magn?tica Nuclear pode ser usada na an?lise de misturas medindo-se o coeficiente de difus?o dos componentes da mistura, permitindo a obten??o dos espectros de cada componente individualmente por meio de um experimento r?pido que n?o interfere na mistura em si. O experimento mais comum ? o DOSY, do ingl?s Espectroscopia de Difus?o Ordenada. Piches, em geral, s?o produtos que s?o empregados como mat?rias-primas na produ??o de uma variedade de materiais de carbono, tais como: anodos de carbono para produ??o de alum?nio, eletrodos de grafite para a ind?stria sider?rgia e fibras de carbono. Piches obtidos a partir do alcatr?o da hulha, res?duo da produ??o de coque metal?rgico, s?o os tradicionalmente mais usados, mas apresentam alto potencial carcinog?nico e tem demanda decrescente em fun??o de novas tecnologias empregadas nas coquerias. Piches de petr?leo, produzidos a partir de ?leos decantados, podem potencialmente constituir uma alternativa mais segura e com maior disponibilidade. O ?leo decantado ? uma fra??o pesada obtida nas unidades de craqueamento catal?tico fluido no refino de petr?leo, e o controle de seu processamento ? dificultado pela complexidade de sua composi??o, que inclui compostos paraf?nicos, homoarom?ticos e heteroarom?ticos. O presente trabalho se prop?em a investigar a an?lise por RMN de uma mistura de padr?es de subst?ncias representativas dos tipos de compostos presentes no ?leo decantado. Assim, foram escolhidos padr?es de Tetracosano, Fenantreno, 9-metil Antraceno, Pireno, Carbazol, 9-metil Carbazol, Dibenzotiofeno, Dibenzofurano, Hexadecano, 1-metil Naftaleno, Tiofeno, Tetrahidrofurano. Todos os experimentos foram realizados no laborat?rio de RMN do PPGQ-UFRRJ a 20?C com o espectr?metro de RMN de 500 MHz Bruker Avance III, equipado com sonda BBO, gradiente no eixo z. Para auxiliar na separa??o dos componentes da mistura foram usados o pol?mero poli-(etilenoglicol), poli-(vinilpirrolidona), poli-(dimetilsiloxano), poli-(metacrilato de butila) e poli-(acetato de vinila), al?m do reagente de deslocamento Eu(fod)3. Os resultados obtidos a partir dos experimentos de DOSY-INEPT mostraram que o pol?mero PMAB foi a matriz que mais auxiliou na separa??o dos componentes da mistura em quest?o no presente trabalho, sendo seguido pelas matrizes PAV, PEG e PVP, que tamb?m apresentaram resultados satisfat?rios