Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-01-26T11:36:13Z No. of bitstreams: 1 2016 - D?bora Barbosa Amarante.pdf: 1603287 bytes, checksum: 8c473e3225022499b4e4dc93119df63f (MD5) / Made available in DSpace on 2017-01-26T11:36:13Z (GMT). No. of bitstreams: 1 2016 - D?bora Barbosa Amarante.pdf: 1603287 bytes, checksum: 8c473e3225022499b4e4dc93119df63f (MD5) Previous issue date: 2016-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The 17?-estradiol (E2) regulate many cardiac genes via its estrogen receptor (ER). The propagation of electrical activity in the myocardium depends on the current transfer at gap junctions. Connexins 40 (Cx40) and 43 are the predominant junctional proteins. In mice, Cx40 is restricted to the atrium and conduction system. Alterations of Cx40 expression or activity are associated with atrial fibrillation. Here we evaluate the effect of the E2 onCx40 mRNA expression, in vitro, and in vivo the effect on atrium expression of Cx40 mRNA in correlation to ECG studies. We treated A7r5 cells (smooth muscle cells from rat thoracic aorta) with low and high doses ofestradiol benzoate, EB, (10-8 M and 10-6M) for 24h and rat neonatal cardiomyocytes with EB 10-6 M for 2, 4 and8 hours. A7r5 cells were trasiently transfected with 0.5 microgramas of the test plasmid (-1190/+121Cx40Luc pGL3) and treated with 10-6M of EB for 24 hours. Female mice were subjected to ovariectomy (OVX) and then, treated with a low (2?g) and a high dose (20?g) of estradiol benzoate (EB; OVX+EB2 and OVX+EB20) for 15 days. ECG recordings were obtained from mice and atrium Cx40 mRNA expression were evaluated by RT-PCR real time. First, we observed that high doses of EB down regulated Cx40 mRNA in vitro (A7r5 cells and rat atrial cardiomyocytes). The transcriptional activity of Cx40 promoter was inhibited by 10-6M of EB in A7r5 cells. We observed lower heart rate for OVX animals when to compared to control animals, FO. In this regard, no difference was observed between OVX+EB2 and OVX+BE20 heart rate. The OVX group showed decrease P wave duration when to compared to FO group, but no difference in the P wave duration was observed among the others groups. No difference was observed in another ECG intervals among the experimental groups. The atrial Cx40mRNA level was reduced in OVX+BE20group when to compared to FO group. Our data indicate, for the first time, high doses of estradiol benzoate reduce Cx40 mRNA in vitro and ovariectomized mice. We proposed that when E2 levels are high, the complex ER-E2 acts directly from the DNA, inhibiting the transcriptional activity of Cx40 promoter / O 17?-estradiol (E2), regula muitos genes card?acos atrav?s de seu receptor (receptor para estr?geno, RE). A propaga??o da atividade el?trica no mioc?rdio depende da transfer?ncia de corrente atrav?s das jun??es comunicantes. As conexinas 40 (Cx40) e 43 s?o as principais conexinas que formam as jun??es expressas no cora??o. Em camundongos, a Cx40 ? restrita ao ?trio e sistema de condu??o. Altera??es na express?o ou atividade da Cx40 est?o associadas a fibrila??o atrial. Aqui n?s avaliamos o efeito do E2 in vitro e in vivo sobre a express?o do RNAm da Cx40 nos ?trios e correlacionamos aos estudos eletrocardiogr?ficos (ECG). N?s tratamos a linhagem A7r5 (derivada de m?sculo liso a?rtico de rato embrion?rio) com alta e baixa concentra??es de benzoato de estradiol, BE, (10-6 M e 10-8 M) durante 24 horas e cultura prim?ria de cardiomi?citos atriais de ratos neonatos foram incubados com BE 10-6 M durante 2, 4 e 8 horas. Ensaios de transfec??o transiente foram realizados na linhagem A7r5 com um plasm?deo contendo um segmento da regi?o promotora da Cx40 (-1190/+121Cx40LucpGL3) e tratadas com 10-6 M de BE durante 24 horas. Camundongos f?meas foram ovariectomizadas (OVX) e ent?o tratadas com baixa (2 microgramas) e alta (20 microgramas) doses de benzoato de estradiol (OVX+BE2 e OVX+BE20) durante 15 dias, sendo que o grupo controle sofreu apenas estresse cir?rgico (FO). Foram realizados registros ECG e a express?o atrial do RNAm da Cx40 foi avaliada por RT-PCR em tempo real. N?s observamos que altas doses de BE diminui a express?o do RNAm da Cx40 in vitro (na linhagem A7r5 e na cultura de cardiomi?citos). A atividade transcricional do promotor foi inibida por BE10-6 M. N?s observamos diminui??o da frequ?ncia card?aca dos animais do grupo OVX em rela??o ao controle, grupo FO. Nenhuma diferen?a foi observada na frequ?ncia card?aca entre os grupos OVX+BE2 e OVX+BE20. Os animais do grupo OVX apresentaram diminui??o na dura??o da onda P em rela??o ao grupo FO, no entanto nenhuma diferen?a foi observada na dura??o da onda P entre os outros grupos. Nenhuma diferen?a foi observada nos outros intervalos eletrocardiogr?ficos entre os grupos experimentais. A express?o atrial do RNAm da Cx40 estava reduzida nos animais do grupo OVX+BE20 em rela??o ao grupo FO. Nossos dados demonstram que altas doses de benzoato de estradiol reduzem a express?o do RNAm da Cx40 in vitro e em camundongos ovariectomizados. N?s propomos que altas doses de E2 ativa o seu receptor, e o complexo RE-E2 age diretamente no DNA, inibindo a atividade transcricional do promotor da Cx40

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2017-03-16T14:03:46Z No. of bitstreams: 1 2008 - Telma de Figueiredo do Nascimento.pdf: 875301 bytes, checksum: 0d53eac4a000372a6c136ed189b7dd48 (MD5) / Made available in DSpace on 2017-03-16T14:03:46Z (GMT). No. of bitstreams: 1 2008 - Telma de Figueiredo do Nascimento.pdf: 875301 bytes, checksum: 0d53eac4a000372a6c136ed189b7dd48 (MD5) Previous issue date: 2008-11-19 / In Brazil it is believed that bovine tuberculosis is present in all states, as is in all continents. The official indices are 1.3% of the national herd infected, which represent a large number in the order of 2.5 million animals. Mycobacterium bovis stands out as zoonotic disease of major importance. Increasing are the economic losses, caused by tuberculosis in cattle, such as low productivity of the herd, condemnation of carcasses at slaughterhouses, reduction in the production of milk and meat, commitment marketing of animals and their products domestically and externally. It is estimated that about 5,0 % of human tuberculosis is caused by Mycobacterium bovis. The success of a program to control a disease is closely linked to various factors including the knowledge of the history of the causative agent and its spatial and temporal distribution. Molecular methods are used as auxiliary tools in combating the disease by providing information in order to achieve these goals. In the last decade, methods for molecular typing of M. tuberculosis were developed and implemented on a large scale. Although the genomes of the two parasites are very similar, the discriminatory power of the methods is smaller in Mycobacterium bovis. In this study were analyzed samples from cattle slaughtered in refrigerators under Federal Inspection in the state of Minas Gerais. A total of 215 cultures sent to the Laboratory of Applied Molecular biology to Micobacterioses, and only 159 confirmed a profile of Mycobacterium bovis through molecular techniques applied. The south and state has the highest prevalence of the disease in the samples studied. The method of Spoligotyping applied to the samples showed the presence of 32 different profiles, obtaining a HGDI of 0.86 and a departure from the MIRU-VNTR presented 40 different profiles with a HGDI of 0.87. In this study was to evaluate that when using these techniques together we get a greater discriminatory power between these strains. / No Brasil acredita-se que a tuberculose bovina est? presente em todos os Estados, assim como est? em todos os continentes. Os ?ndices oficiais est?o em 1,3% do rebanho nacional infectado, que representaria um n?mero elevado, na ordem de 2,5 milh?es de animais. Mycobacterium bovis destaca-se como zoonose de reconhecida import?ncia. Crescentes s?o as perdas econ?micas, causada pela tuberculose em bovinos, como a baixa na produtividade do rebanho, condena??o de carca?as em matadouros, redu??o na produ??o de leite e carne, comprometimento da comercializa??o de animais e seus produtos no mercado interno e externo. Estima-se que em torno de 5,0% da tuberculose humana ? causada por M. bovis. O sucesso de um programa de controle de uma doen?a est? estreitamente ligado a diferentes fatores entre os quais o conhecimento da hist?ria do agente etiol?gico e sua distribui??o espacial e temporal. Os m?todos moleculares s?o utilizados como ferramentas auxiliares no combate ? doen?a fornecendo informa??es com a finalidade de alcan?ar estes objetivos. Na ?ltima d?cada, m?todos para tipagem molecular de Mycobacterium tuberculosis foram desenvolvidos e aplicados em larga escala. Embora o genoma dos dois parasitos seja muito parecido, o poder discriminat?rio dos m?todos decresce para Mycobacterium bovis. Neste trabalho foram analisadas amostras de bovinos abatidos em Frigor?ficos sob Inspe??o Federal no estado de Minas Gerais. Um total de 215 culturas foram enviadas ao Laborat?rio de Biologia Molecular Aplicada ? Micobacterioses, e apenas 159 confirmaram um perfil de Mycobacterium bovis atrav?s das t?cnicas moleculares de Spoligotyping e 159 a t?cnica do MIRU-VNTR. A mesorregi?o Sul e Sudoeste mostraram a maior preval?ncia da doen?a nas amostras estudadas. O m?todo de Spoligotyping aplicados ?s amostras mostrou a presen?a de 32 perfis diferentes, obtendo um HGDI de 0,86 e em contra partida o MIRU-VNTR apresentou 40 perfis diferentes com um HGDI de 0,87. Neste estudo foi poss?vel avaliar que quando utilizamos estas t?cnicas em conjunto obtemos um maior poder discriminat?rio entre essas cepas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-03-27T13:44:49Z No. of bitstreams: 1 2010 - ?rica Gaspar da Silva.pdf: 488338 bytes, checksum: 0fb8f4161c16ffb2272dd0dd607a0828 (MD5) / Made available in DSpace on 2017-03-27T13:44:49Z (GMT). No. of bitstreams: 1 2010 - ?rica Gaspar da Silva.pdf: 488338 bytes, checksum: 0fb8f4161c16ffb2272dd0dd607a0828 (MD5) Previous issue date: 2010-07-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The maned wolf, Chrysocyon brachyurus, the largest canid of South America, is often characterized as an omnivorous animal because of its diet in the wild, which includes plants and animals. Thus, it is considered a generalist species. Studies about the digestive physiology of this animal are rare, mainly due to the importance of the issue to the support of adequated nutritional manipulation that makes possible the survival of the species. In this sense, the objective of this study is assess the ability of the maned wolf to digest typicals fruits of Cerrado, Solanum lycocarpum (wolf?s fruit) and Annona crassiflora, and characteristics of its diet in the wild as well as examine the digestibility of a mixed diet of rats and quails, representing the animal portion of its diet. The maned wolf has a good ability to digest fruits, reinforcing its characterization as an omnivorous animal, and the fruits are great importance to the processes of nutrition of the species. The study confirmed the enormous importance of wolf?s fruit in the diet of this animal. Besides this, that fruit presented high digestibility by animals tested, and cause of this maned wolf?s fruit is probably very important during the dry period, where the availability of fruits, in general, is lower. The digestibility of dry material of wolf?s fruit is even greater than that of a mixed diet with animal and plant fraction, confirming the importance of that fruit to the nutrition of the maned wolf / O lobo-guar?, Chrysocyon brachyurus, maior can?deo da Am?rica do Sul, ? freq?entemente caracterizado como um animal on?voro devido a sua dieta em vida livre, que inclui itens de origem animal e vegetal, sendo uma esp?cie generalista. Estudos sobre a fisiologia digestiva desse animal s?o raros, principalmente frente ? import?ncia do tema para o suporte do manejo nutricional adequado que viabilize a sobreviv?ncia da esp?cie. Nesse sentido, o objetivo do presente trabalho ? avaliar a capacidade do lobo-guar? de digerir algumas frutas t?picas do Cerrado, como por exemplo, Solanum lycocarpum (?fruta do lobo?) e Annona crassiflora (?Araticum?), que s?o caracter?sticas da sua dieta em vida livre. An?lise da digestibilidade de uma dieta mista com ratos e codornas que representem a por??o animal da sua alimenta??o. O lobo-guar? possui boa capacidade de digerir frutos, refor?ando sua caracteriza??o como um animal on?voro, e esses frutos s?o de grande import?ncia para os processos de nutri??o da esp?cie. O estudo confirmou a relev?ncia da ?fruta do lobo?na dieta dos animais, apresentando elevada digestibilidade por parte dos indiv?duos testados, sendo provavelmente muito importante durante o per?odo de seca, onde a disponibilidade de frutos, de forma geral, ? mais baixa. A digestibilidade da mat?ria seca da ?fruta do lobo? ? ainda maior do que de uma dieta mista com fra??o animal e vegetal, comprovando a import?ncia desse fruto para a nutri??o desses animais.

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2017-04-24T14:23:01Z No. of bitstreams: 1 2016 - Carlos Augusto dos Santos Sousa.pdf: 2340153 bytes, checksum: 5ef373f242c2c4700a9a9e55280bc62c (MD5) / Made available in DSpace on 2017-04-24T14:23:01Z (GMT). No. of bitstreams: 1 2016 - Carlos Augusto dos Santos Sousa.pdf: 2340153 bytes, checksum: 5ef373f242c2c4700a9a9e55280bc62c (MD5) Previous issue date: 2016-02-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / Morphology studies provide knowledge that allow us to understand how animals interact with the natural environment or in captivity. In this context, the comparative anatomy of the formation of the brachial plexus awakens interest since the nineteenth century and remains one of the most intriguing topics of contemporary anatomy. The aim of this study was to describe the origin and the antimeric distribution of the brachial plexus nerves in Macaca mulatta, as well as the innervated muscles. Ten male rhesus monkeys (Macaca mulatta) were used, from the Non-human Primates? Breeding Department at the Laboratory Animals Breeding Centre (Cecal/Fiocruz), donated to the Animal Anatomy Department of the Rural Federal University of Rio de Janeiro (UFRRJ). The specimens were fixed in formaldehyde solution by infusion of 10% solution. They were subsequently wrapped in a low-density polythene container with 500 liters of formaldehyde 30% solution over a period of 12 months. After this period, they were washed in running water and subjected to X-ray examinations of the neck at the Small Animals Veterinary Hospital of the UFRRJ to characterize the number of cervical vertebrae. Then, they had both antimeres dissected aiming at the exposure of the origins and the nerves arising from the brachial plexus. Data were presented both in absolute frequency and in simple percentage. In 11 (55%) animals the resulting nerves were constituted by the connections between the ventral spinal branches C5, C6, C7, C8 and T1. In 5 (25%) animals, the participants roots were C4, C5, C6, C7, C8, T1 and T2. In 2 (10%) animals C5, C6, C7, C8, T1 and T2. In the other 2 (10%) animals the formation of the plexus was observed from C6, C7, C8, T1 and T2. The ventral branches formed three nerve trunks: cranial, middle and caudal. The suprascapular nerves, subscapular, axillary, musculocutaneous, radial, median, ulnar innervated the intrinsic muscles and the subclavian nerve innervated the thoracodorsal, medial cutaneous arm and forearm, long thoracic, cranial pectoral and caudal pectoral innervate extrinsic muscles. The results obtained in this study contribute to the comparative anatomy of primates and to the information for applied research, serving as basis for clinical and surgical procedures that uses this species as an animal model. / Estudos morfol?gicos fornecem conhecimentos que permitem entender o modo como os animais interagem com o ambiente natural ou em cativeiro. O objetivo desse estudo foi descrever a origem e a distribui??o antim?rica dos nervos do plexo braquial em Macaca mulatta, assim como dos m?sculos inervados. Foram utilizados 10 cad?veres de Macaca mulatta do sexo masculino, oriundos do Servi?o de Cria??o de Primatas N?o Humanos do Centro de Cria??o de Animais de Laborat?rio (Cecal/Fiocruz) doados a ?rea de Anatomia Animal da Universidade Federal Rural do Rio de Janeiro (UFRRJ). Os esp?cimes foram fixados com perfus?o de solu??o de formalde?do a 10%. Posteriormente, foram acondicionados em caixas de polietileno de baixa densidade com capacidade de 500 litros contendo solu??o de formalde?do a 30% por um per?odo de 12 meses. Ap?s este per?odo, foram lavados em ?gua corrente e submetidos a exames radiogr?ficos da regi?o cervical no Hospital Veterin?rio de Pequenos Animais da UFRRJ para a caracteriza??o do n?mero de v?rtebras cervicais. Em seguida, foram dissecados at? a exposi??o das origens e dos nervos oriundos do plexo braquial. Os dados foram representados em frequ?ncia absoluta e percentual simples. Em 11 (55%) os nervos resultantes foram constitu?dos das conex?es entre os ramos espinhais ventrais de C5, C6, C7, C8 e T1. Em 5 (25%) as ra?zes participantes foram C4, C5, C6, C7, C8, T1 e T2. Em 2 (10%) de C5, C6, C7, C8, T1 e T2. Em outros 2 (10%) verificamos a constitui??o do plexo a partir de C6, C7, C8, T1 e T2. Os ramos ventrais formaram tr?s troncos nervosos: cranial, m?dio e caudal. Os nervos supraescapular, subescapulares, axilar, musculocut?neo, radial, mediano, ulnar inervaram a musculatura intr?nseca e os nervos subcl?vios, toracodorsal, tor?cico longo, peitoral cranial e peitoral caudal inervaram a musculatura extr?nseca. Tamb?m foram registrados os nervos cut?neos oriundos do plexo braquial, sendo eles o nervo cut?neo medial do bra?o, nervo cut?neo medial do antebra?o e ramos para a musculatura cut?nea do tronco. Os dados descritos neste estudo contribuem para a anatomia comparada de primatas e fornecem informa??es para a pesquisa aplicada, servindo como base para procedimentos cl?nico-cir?rgicos em que venha a se utilizar esta esp?cie como modelo experimental.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-22T12:42:37Z No. of bitstreams: 1 2016 - Ericson Ramos de Mello.pdf: 4027891 bytes, checksum: 7ce6fcb724a96319669aef0ec318a39e (MD5) / Made available in DSpace on 2017-09-22T12:42:37Z (GMT). No. of bitstreams: 1 2016 - Ericson Ramos de Mello.pdf: 4027891 bytes, checksum: 7ce6fcb724a96319669aef0ec318a39e (MD5) Previous issue date: 2016-09-29 / The Centers for Wild Animals Screening (CETAS) on jurisdiction of the Brazilian Institute of Environment and Renewable Resources (IBAMA), are responsible for the receipt and disposal of wild animals that are victims of trafficking throughout the national territory. The CETAS of Serop?dica (CETAS-RJ) is the only place responsible for the receipt and disposal of wild animals that are victims of trafficking in the state of Rio de Janeiro. This study aimed to characterize the species of wildlife, seized or voluntarily surrendered, 2008-2014 in CETAS of Serop?dica ? Rio de Janeiro. The total effective entries in the Wild Animal Screening Center was 39.777 (thirty-nine thousand, seven hundred seventy-seven) animals during the years 2008 to 2014, with the annual average of the last 7 years, 5.682 entries. Among the animals received, 91,25% (36.295) were birds, 5,47% (2.177) reptiles, 3,14% (1.248) mammals, 0,13% (53) invertebrates; between crustaceans, insects and arachnids and 0,01% (4) indeterminate information from seizures, deliveries or redemptions. The seizure was the origin most represented during the study period, with 88,75% (35.302) animals and an average of 5.043 animal per year. The Environmental Police Command (CPAM), along with other battalions of military and Civilian Police were the most representative bodies in the fight against wildlife trafficking. Receipt of birds between the years, identified 24 orders, 54 families and 255species of birds. The order Passeriformes was the one that had the highest number of incoming animals (92,22%), having predominance of Thaupidae family with 85,54% (28.634), followed by family Icteridae 3,41% (1.143). The collared (Sporophila caerulescens), the Saffron Finch (Sicalis flaveola), the Crack-iron-true (Saltator similis), the Songbird (Sporophila angolensis), accounted for about 78,18% of passerines, 61,15% of the seized birds and 56,60% of total birds received in CETAS ? RJ, during the study period / Os Centros de Triagem de Animais Silvestres (CETAS), sob jurisdi??o do Instituto Brasileiro de Meio Ambiente e Recursos Renov?veis (IBAMA), s?o respons?veis pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico em todo territ?rio Nacional. O CETAS de Serop?dica (CETAS-RJ), localizado na Floresta M?rio Xavier ? o ?nico local respons?vel pelo recebimento e destina??o dos animais silvestres que s?o v?timas do tr?fico no Estado do Rio de Janeiro. Objetivou-se caracterizar as esp?cies, da fauna silvestre, apreendidas ou entregues voluntariamente, de 2008 a 2014 no CETAS de Serop?dica ? Rio de Janeiro. O total de entradas efetivas no Centro de Triagem de Animais Silvestres foi de 39.777 (Trinta e nove mil, setecentos e setenta e sete) animais, durante os anos de 2008 a 2014, tendo como m?dia anual, dos ?ltimos 7 anos, 5.682 entradas. Dentre os animais recebidos, 91,25% (36.295) foram aves, 5,47% (2.177) r?pteis, 3,14% (1.248) mam?feros, 0,13% (53) invertebrados; entre crust?ceos, insetos e aracn?deos provenientes de apreens?es, entregas ou resgates. A apreens?o foi a proced?ncia com maior representa??o durante o per?odo estudado, com 88,75% (35.302) animais e uma m?dia de 5.043 animais por ano. O Comando de Pol?cia Ambiental (CPAM), juntamente com outros batalh?es da pol?cia militar e civil, foram os ?rg?os mais representativos no combate ao tr?fico de animais silvestres. O recebimento de aves entre 2008 e 2014 representou cerca de 91,25% (36.295) das entradas em rela??o ao total de todas as classes entre os anos, sendo identificadas 24 ordens, 54 fam?lias e 255 esp?cies de aves. A ordem Passeriformes foi a que teve o maior n?mero de animais recebidos (92,22%), havendo predomin?ncia das fam?lias, Thaupidae com 85,54% (28.634) e Icteridae 3,41% (1.143). O coleirinho (Sporophila caerulescens), o can?rio-da-terra-verdadeiro (Sicalis flaveola), o trinca-ferro-verdadeiro (Saltator similis), o curi? (Sporophila angolensis), o tiziu (Volatinia jacarina), o tico-tico (Zonotrichia capensis), corresponderam a cerca de 78,18% do total de Passeriformes, 61,15% apreendidos e 56,60% das aves recebidas no CETAS - RJ, no per?odo do estudo.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-03T16:25:38Z No. of bitstreams: 1 2016 - Viviane Figueira Marques.pdf: 1883187 bytes, checksum: 9996d5ea41ecd4f93424c808c4d82ba6 (MD5) / Made available in DSpace on 2017-10-03T16:25:38Z (GMT). No. of bitstreams: 1 2016 - Viviane Figueira Marques.pdf: 1883187 bytes, checksum: 9996d5ea41ecd4f93424c808c4d82ba6 (MD5) Previous issue date: 2016-03-02 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Staphylococcus spp. plays an important role in the etiology of bovine mastitis. Staphylococcus aureus is considered the most relevant specie due to the production of virulence factors such as ?slime?, which is required for biofilm formation. This study aimed to detect the phenotypic expression of the biofilm formation in 20 S. aureus isolates from bovine mastitis, to detect and quantify the expression of genes involved in its production and regulation, as well as to detect the phenogenotypic resistance to beta-lactam in order to evaluate the possible relation between biofilm production and antimicrobial resistance. The isolates were characterized by MALDI-TOF and phenogenotypic identification assays. Also they were submitted to the phenotypic tests to evaluate biofilm production and the susceptibility to beta-lactams. Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration in the Biofilm (MICB) were determined to three isolates presenting distinct biofilm production. Futherly, a PCR for the detection of ?slime? production genes (icaA and icaD), Bap protein (bap), beta-lactamase (blaZ) and protein altered penicillin-binding (mecA). Also, the Agr system was typified (agr I, agr II, agr III and agr IV) and its regulator (agr RNAIII) was detected. Scanning Electron Microscopy (SEM) was performed to determine the most suitable time interval for biofilm analysis. Real-time PCR (qPCR) was performed at the chosen times to quantify the expression of icaA, icaD and hld genes in the three studied isolates. All 20 isolates were biofilm producers and most presented icaA and icaD genes. Only one isolate presented the bap gene. The agr gene type II presented a prevalence of 70%. The SEM showed gradual changes in bacterial arrangement during the biofilm formation along the phases of the growth curve. The peak was reached at the stationary phase. Transcriptional analysis revealed increased expression of ica genes at 8 h of growth and of hld at 24 h. However, for the N-365 strain the ica expression was of low yield. For this study, the penicillin resistance was related to the production of beta-lactamase otherwise the high MBC detected for cefoxitin may be associated to biofilm protection, evidentiated by the fact that the isolates have MICB values higher than MICs tested for planktonic cells / Staphylococcus spp. tem papel importante na etiologia da mastite bovina. Staphylococcus aureus ? considerada a esp?cie mais relevante devido ? produ??o de fatores de virul?ncia, tais como ?slime?, o que favorece a forma??o do biofilme. O presente trabalho teve por objetivo detectar a express?o fenot?pica da forma??o de biofilme em 20 isolados de S. aureus oriundos de mastite bovina, detectar e quantificar a express?o dos genes envolvidos na sua produ??o e regula??o, al?m de detectar a resist?ncia fenogenot?pica aos beta-lact?micos para avalia??o da poss?vel rela??o da produ??o de biofilme com a resist?ncia antimicrobiana. Os isolados foram caracterizados atrav?s de testes fenogenot?picos e MALDI-TOF, submetidos ?s provas fenot?picas de detec??o da forma??o de biofilme e avalia??o da suscetibilidade aos beta-lact?micos. A Concentra??o Inibit?ria M?nima (CIM), Concentra??o Bactericida M?nima (CBM) e a Concentra??o Inibit?ria M?nima no Biofilme (CIMB) foram determinadas para tr?s isolados selecionados com base na varia??o da intensidade da produ??o de biofilme. Posteriormente, todos os isolados foram submetidos ? t?cnica de PCR para detec??o dos genes de produ??o de ?slime? (icaA e icaD), prote?na Bap (bap), beta-lactamase (blaZ) e prote?na ligante de penicilina alterada (mecA). Al?m de detec??o do sistema regulador Agr (agr RNAIII) e da tipifica??o do sistema Agr (agr I, agr II, agr III e agr IV). Foi realizada Microscopia Eletr?nica de Varredura (MEV) para determinar o intervalo de tempo mais adequado para a an?lise do biofilme. A PCR em tempo real (qPCR) foi realizada nos tempos selecionados para quantificar a express?o dos genes icaA, icaD e hld em tr?s isolados com produ??o variada de biofilme. Todos os isolados foram produtores de biofilme e a maioria apresentou os genes icaA e icaD. Apenas um isolado apresentou o gene bap. O gene agr tipo II mostrou preval?ncia de 70%. A MEV mostrou mudan?as graduais no arranjo bacteriano durante a forma??o de biofilme ao longo das fases da curva de crescimento que atingiu seu pico de forma??o na fase estacion?ria. A an?lise transcricional evidenciou maior express?o dos genes ica no tempo de 8 h de crescimento e hld em 24 h. Contudo, a cepa N-365 mostrou baixa produ??o dos genes ica. Para este estudo, a resist?ncia ? penicilina foi relacionada com a produ??o de beta-lactamase, enquanto a elevada CBM detectada para cefoxitina pode estar associada ? prote??o que o biofilme oferece, epis?dio evidenciado pelo fato dos isolados apresentarem valores de CIMB superiores aos CIMs testados para as c?lulas planct?nicas.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2017-10-05T13:34:35Z No. of bitstreams: 1 2009 - Claudio Augusto Ribeiro de Souza.pdf: 21273946 bytes, checksum: 90399a81f4785e952e86f0792d34dac4 (MD5) / Made available in DSpace on 2017-10-05T13:34:35Z (GMT). No. of bitstreams: 1 2009 - Claudio Augusto Ribeiro de Souza.pdf: 21273946 bytes, checksum: 90399a81f4785e952e86f0792d34dac4 (MD5) Previous issue date: 2009-05-04 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES, Brasil. / The genus Rhopalurus is reviewed in South America based on external morphology and hemispermatophore morphology of the males. Three species and two subspecies are synonymized: Rhopalurus amazonicus with R. laticauda, R. crassicauda with R. laticauda, R. acromelas with R. agamemnom, R. crassicauda paruensis with R. laticauda,R. pintoi kouruensis with R. pintoi. The genus present now seven valid species for South America. New data on distribution range and an identification key are presented. A comparative analysis of the morphology of male hemiespermatophore of 21 genera of the family Buthidae of Central and South America, Europe, Africa and Asia. The implications of these new data on the taxonomic organization of the family Buthidae are discussed. / O g?nero Rhopalurus ? revisado na Am?rica do Sul com base na morfologia externa e na morfologia do hemiespermat?foro dos machos. Tr?s esp?cies e duas subesp?cies s?o sinonimizadas: Rhopalurus amazonicus com R.. laticauda, R. crassicauda com R. laticauda, R. acromelas com R. agamemnom, R. crassicauda paruensis com R. laticauda, R. pintoi kouruensis com R. pintoi. O elenco do g?nero passa a apresentar sete esp?cies v?lidas para Am?rica do Sul. Novos dados de distribui??o e uma chave de identifica??o s?o apresentados. ? realizada a an?lise comparativa da morfologia dos hemiespermat?foros dos machos de 21 g?neros da fam?lia Buthidae das Am?ricas Central e Sul, Europa, ?frica e ?sia. S?o discutidas as implica??es destes novos dados na composi??o taxon?mica da fam?lia Buthidae.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-18T12:35:34Z No. of bitstreams: 1 2016 - Tatiana Pires Pereira.pdf: 2546753 bytes, checksum: c1673b0ae2c6812a3f60faa79b5fae75 (MD5) / Made available in DSpace on 2017-10-18T12:35:34Z (GMT). No. of bitstreams: 1 2016 - Tatiana Pires Pereira.pdf: 2546753 bytes, checksum: c1673b0ae2c6812a3f60faa79b5fae75 (MD5) Previous issue date: 2016-07-27 / Funda??o Carlos Chagas Filho de Amparo ? Pesquisa do Estado do RJ - FAPERJ / This work was divided in four chapters, in which the first was performed with the goal of quantifying the condensed tannin (CT) content by the Stiasny?s reaction and to determine the classes of secondary metabolites present by the phytochemical prospection technique and magnetic resonance in the tropical forage legumes Cajanus cajan (guandu - GUA), Gliricidia sepium (gliricidia - GLI), Flemingia macrophylla (flemingia - FLE), Cratylia arg?ntea (cratilia - CRA), Mimosa caesalpineafolia (sabi?) (this legume divided into bark and leaf fraction ? SABc and SABf) among the treatments. The extracts obtained were divided into: total extract, number of Stiasny (NS), CT and non-tannins. The FLE, CRA, GUA,GLI, SABf and SABc had obtained the following values for total extract: 13.20; 13.06; 8.28; 14.73; 15.67 and 6.22%, respectively. The reactivity by NS, in the same order of legumes, was 11.25; 4.54; 7.37; 6.70; 23.06 and 71.62%, whereas the CT presented the following values: 1.52; 0.59; 0.61; 0.96; 3.6 and 4.43%, and non-tannin was 11.68; 12.46; 7.67; 13.75; 12.07 and 1.76%, respectively. The following classes of secondary metabolites were identified with greater evidence: saccharides, carbohydrates, non-protein amino acids and glicos?deos cardioativos. For the CT, the intensity was low for most of the legumes, with greater content in CRA, GUA and SABf. The wain compound in the extracts was methyl-inositol (sugar). The second chapter had the objective of assaying in the legumes mentioned above and one more specie, Stylosanthes spp. (estilosantes-EST), condensed tannin (CT) constituents, with the use of organic solvents, soluble CT (ECT), CT adhered to protein (PBCT), CT adhered to fiber (FBCT), and total CT (TCT), CT structural pro-pelargonidin (PP); prodelfinidin (PD) and procyanidin (PC), molecular weight (polymerization degree (DP), molecular distance distributed of the polymer (PDI); average weight of molecular mass (Mw), and average number of molecular mass (Mn), and the biological activity through precipitated proteins by phenols (PPP). The variables ECT, PBCT, and TCT presented were influenced by different species (P?0.05). The FBCT fraction was not found in the legumes. Molecular weights (DP, PDI, Mw e Mn) were affected by the different species (P?0.05), ranging from 737 to 1168 da. The structural characteristics (PP, PD, PC and PD:PC) varied among the species. In the third chapter I evaluated methanogenesis (total methane (CH4total)), incubated (CH4inc) and fermented (CH4ferm) and ruminal fermentation parameters total gas production (PGT), pH, ammonium (N-NH3), short-chain fatty acids (SCFA) and in vitro organic matter disappearance (IVOMD) as they related to CT present in the legumes and Urochloa brizantha cv. marandu hay as control (CTL). The effect of polyethylene glycol (PEG) on the leaf fraction of sabi? was tested as well, which had a CT content of 15.97%. No alteration in the pH (P?0.05) for the treatments evaluated. However, a decrease of total gas and methane production for all the treatments with presence of CT (P?0.05). When PEG was added, there was a 27.01 (8% PEG) and 35.01 (16% PEG) increase in total gas production and 3.59 (8% PEG) and 4.15 (16% PEG) of methane production. GUA, FLE, SABc and SABf were capable of modifying (P?0.05) the content of NH3-N (mg/dL), along with the CTL, which also presented lower values compared to legumes with no or only traces of CT (ETL, CRA and GLI). There was significant difference (P?0.05) for IVOMD between the legumes and control, it was observed lower disappearance (P?0.05) for FLE, GUA and SABf in relation to CTL, while SABc did disappear. The SABf IVOMD was affected by the addiction of PEG. There was lower digestibility for FLE, GUA and SABf in relation to the CTL, while the bark fraction of SAB did not disappear at all. On the SCFA profile, there was difference (P?0.05) among the treatments evaluated, with lower values for the legumes with presence of CT. In the fourth chapter I tested the effect of CT from the legumes in study (FLE, CRA, GUA, GLI, EST, SABf and SABc) on larval migration inhibition (LMI) in vitro, on the infective larvae L3 of the nematode Haemonchus contortus (HC), compared with Ivermectin and a negative control (rumen fluid and buffer). Among the legumes studied, SABf and GUA did not differ (P?0.05), with the greater (P?0.05) LMI percentage (34.75% and 34.33%) than the other entries. The legumes GUA, FLE and SABc did not differ (P?0.05), presenting moderate values of LMI (30.25%, 30.0% and 29.75%, respectively). Among the legumes studied, the lowest LMI percentage was CRA (18.46%), GLI (23.75%) and negative control (rumen fluid and buffer), with values near (P?0.05) from to Ivermectin (22.0%). / Este trabalho foi dividido em quatro cap?tulos. O primeiro realizou-se com o objetivo de quantificar o teor de tanino condensado (TC) atrav?s da Rea??o de Stiasny e conhecer as classes de metab?litos secund?rios presentes pela t?cnica de prospec??o fitoqu?mica e resson?ncia magn?tica nas leguminosas forrageiras tropicais Cajanus cajan (guandu-GUA), Gliricidia sepium (gliricidia-GLI), Flemingia macrophylla (flemingia-FLE), Cratylia arg?ntea (cratilia-CRA), Mimosa caesalpineafolia (sabi?) sendo que essa leguminosa tinha a fra??o casca e folha (SABc e SABf) entre os tratamentos. Os extratos obtidos foram divididos em: extrato total, n?mero de Stiasny (NS), TC e n?o taninos. A FLE, CRA, GUA, GLI, SABf e SABc apresentaram os valores para o extrato total 13,20; 13,06; 8,28; 14,73; 15,67 e 6,22%, respectivamente. A reatividade pelo NS, na mesma ordem das leguminosas, foi de 11,25; 4,54; 7,37; 6,70; 23,06 e 71,62%, j? o TC apresentou os seguintes valores 1,52; 0,59; 0,61; 0,96; 3,6 e 4,43% e o n?o tanino foi de 11,68; 12,46; 7,67; 13,75; 12,07 e 1,76%, respectivamente. Foram identificadas as seguintes classes de compostos secund?rios em maiores evid?ncias: os sacar?deos, carboidratos, amino?cidos n?o prot?icos e os glicos?deos cardioativos. J? para o TC, a intensidade foi baixa para grande parte das leguminosas, prevalecendo maior teor para CRA, GUA e SABf. Foi constatado como componente principal nos extratos o metil-inositol (a??car). O segundo cap?tulo teve como objetivo avaliar nas leguminosas citadas acima e mais uma esp?cie, o Stylosanthes spp (estilosantes-EST), analisar os constituintes do TC com uso de solvente org?nico, tanino sol?vel (TCE), tanino aderido ? prote?na (TCPB), tanino aderido ? fibra (TCFB) e taninos condensados totais (TCT), caracter?sticas estruturais tais como: propelargonidina (PP); prodelfinidina (PD) e procianidina (PC); peso molecular (grau de polimeriza??o?(DP); dist?ncia do peso molecular distribu?do do pol?mero (PDI); peso m?dio da massa molecular (Mw); n?mero m?dio da massa molecular (Mn); al?m de determinar a atividade biol?gica, atrav?s da t?cnica de prote?nas precipit?veis por fen?is (PPP). As vari?veis TCE, TCPB e TCT apresentadas foram influenciadas pelas diferentes esp?cies (P?0,05). A fra??o TCFB n?o foi constatada nas leguminosas. Os pesos moleculares (Mw) foram influenciados pelas diferentes esp?cies (P?0,05), variando de 737 a 1168 Da. As caracter?sticas estruturais (PP, PD, PC e PD:PC) tiveram varia??o entre as esp?cies estudadas. Objetivou-se com o terceiro cap?tulo avaliar a metanog?nese (metano total (CH4-total), incubado (CH4 inc.) e fermentado (CH4 ferm.) e os par?metros de fermenta??o ruminal (produ??o de g?s total (PGT), pH, am?nia (N-NH3), ?cidos graxos de cadeia curta (AGCC) e digestibilidade in vitro da mat?ria org?nica (DIVMO) frente aos TC presentes nas leguminosas e feno de Urochloa brizantha cv. marandu como controle (CTL). Foi testado tamb?m o efeito do polietileno glicol (PEG) sobre a fra??o folha do sabi?, que teve conte?do de TC de 15,97%. N?o foi observado altera??o no pH (P?0,05) para os tratamentos avaliados. No entanto, foram observadas diminui??o da produ??o total de g?s e produ??o de metano para todos os tratamentos com presen?a de TC (P?0,05). Para o tratamento com PEG houve aumento de 27,01 (8% PEG) e 35,01 (16% PEG) na produ??o total de g?s e 3,59 (8% PEG) e 4,15 (16% PEG) na produ??o de metano. GUA, FLE, SABc e SABf foram capazes de modificar (P?0,05) a concentra??o de N-NH3 (mg/dL) juntamente com o CTL, que tamb?m apresentou valores inferiores comparado as leguminosas com tra?os e aus?ncia do TC (ETL, CRA e GLI). Houve diferen?a (P?0,05) para DIVMO entre as leguminosas e o controle, observou-se menor digestibilidade (P?0,05) para FLE, GUA e SABf, em rela??o ao CTL, n?o sendo digest?vel o SABc. A DIVMO foi afetada pela adi??o de PEG na dieta do SABf. No perfil dos AGCC houve diferen?a (P?0,05) para os tratamentos avaliados, com menor valor para as leguminosas com presen?a de TC. O quarto cap?tulo teve como objetivo testar o efeito da t?cnica de inibi??o da migra??o larval (IML) in vitro do TC proveniente das leguminosas em estudo (FLE, CRA, GUA, GLI, EST, SABf e SABc) sobre as larvas infectantes L3 do nemat?de o Haemonchus contortus (HC) comparando com Ivermectina e controle negativo (l?quido ruminal e tamp?o). Entre as leguminosas estudadas o SABf e GUA n?o diferiram entre si (P?0,05), com as maiores porcentagens IML (34,75% e 34,33%). As leguminosas GUA, FLE e SABc n?o diferiram entre si (P?0,05), apresentando moderados valores de IML (30,25%, 30,0% e 29,75%, respectivamente). Entre as leguminosas estudadas a menor porcentagem de IML foi para CRA (18,46%), GLI (23,75%) e controle negativo (l?quido de r?men e tamp?o) valores pr?ximos do controle positivo com Ivermectina (22,0%).

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-10-19T10:50:10Z No. of bitstreams: 1 2016 - Silvio Rodrigues da Silva.pdf: 1225497 bytes, checksum: d2f9db78af36de26e8b7a7201b2e6407 (MD5) / Made available in DSpace on 2017-10-19T10:50:10Z (GMT). No. of bitstreams: 1 2016 - Silvio Rodrigues da Silva.pdf: 1225497 bytes, checksum: d2f9db78af36de26e8b7a7201b2e6407 (MD5) Previous issue date: 2016-07-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / It evaluated the prevalence and potential risk factors for the occurrence of ticks in dogs from January 2000 to December 2014 in two private veterinary clinics, located in the West Zone of Rio de Janeiro state, Brazil. Fifteen hundred records were systematically sampled from an unknown population. Fifteen hundred records were systematically sampled from an unknown population. Statistical analysis was performed considering the outcome variable, tick presence (group 1) and positive tick + negative tick / positive hemoparasite (group 2) and the explanatory variables, sex, age, race, size, neighborhood, and clinic. An exploratory analysis was made to verify the distribution of the prevalence of ticks and their range of 95% confidence, considering all the explanatory variables for the presence of ticks. Differences in the prevalence also were testing by reason proportions test. Bivariate and multivariate analysis by logistic regression models were using to verify the relationship of the outcome of interest and their potential risk factors through the prevalence ratio (PR). The adopted modeling strategy was based on the bivariate analysis. After then, variables were selected to be included in the multivariate model. Akaike Information Criterion (AIC) were estimated to indicated the most parsimonious multivariate model. All statistical analysis was performed in the statistical package R. Of all the 1,500 records of dogs in 264 (17.60% [95% CI: 15.67, 19.52]) had the infestation by ticks in group 1 and 372 (24.80% [95% CI: 22 61, 26.98]) in group 2. Sex and age were not considered risk factors. There were differences in parasitism between races within groups 1 and 2. Males Cocker Spaniel, medium-sized and Bangu were the variables with the highest prevalence in both groups. German Shepherd race does not configured risk factor for ticks, confirmed by the most parsimonious model (PR = 1.77 [95% CI: 0.44, 07.10]). The Cocker Spaniels as well as animals living in Bangu had 7.5 [95% CI: 3.01, 18.53] and 1.7 [95% CI: 1.20, 2.32] times, respectively, more chance to be parasitized by ticks. The prevalence of hemoparasite transmitted by ticks should be considered as supplementary data for ticks epidemiological studies in dogs. This is the first study to rank the dog breeds in relation to the prevalence of parasitism by ticks. / Foi avaliada a preval?ncia e os potenciais fatores de risco para a ocorr?ncia de carrapatos em c?es no per?odo de janeiro de 2000 ? dezembro de 2014 em duas cl?nicas veterin?rias particulares, localizadas na zona oeste do Rio de Janeiro, Brasil. Mil e quinhentos prontu?rios foram amostrados sistematicamente de uma popula??o desconhecida. A an?lise estat?stica foi realizada considerando as vari?veis desfecho, presen?a de carrapato (grupo 1) e carrapato positivo + carrapato negativo/ hemoparasito positivo (grupo 2) e as vari?veis explicativas, sexo, idade, ra?a, porte, bairro e cl?nica. Para verificar a exist?ncia de fatores de risco, foram utilizadas desde testes de infer?ncia de Qui-Quadrado (??) at? o ajuste de modelos de regress?o log?sticas. Dos 1500 prontu?rios em 264 (17,60% [IC95%: 15,67; 19,52]) haviam registro de infesta??o por carrapatos no grupo 1 e 372 (24,80% [IC95%: 22,61; 26,98]) no grupo 2. As vari?veis sexo e idade, em ambos os grupos n?o foram consideradas potenciais fatores de risco. Houve diferen?a no parasitismo entre as ra?as dentro dos grupos 1 e 2. Machos de Cocker Spaniel Ingl?s, porte m?dio e Bangu foram as vari?veis com maiores preval?ncias de registros em ambos os grupos. Pastor Alem?o foi a ?nica ra?a que n?o configurou como potencial fator de risco para a presen?a de carrapato. No modelo mais parcimonioso apenas a ra?a Pastor Alem?o (RP=1,77 [IC95%:0,44; 07,10]) n?o se apresentou como potencial fator de risco. A ra?a Cocker Spaniel assim como animais que residem no bairro Bangu apresentaram 7,5 [IC95%:3,01;18,53] e 1,7 [IC95%:1,20; 2,32] vezes, mais chances de serem parasitados por carrapatos respectivamente. A preval?ncia de hemoparasitoses transmitidas por carrapatos deve ser considerada como dados complementares para estudos epidemiol?gicos de carrapatos em c?es. Esse ? o primeiro estudo a ranquear ra?as de c?es quanto ? preval?ncia de parasitismo por carrapatos.

Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-11-09T10:53:09Z No. of bitstreams: 1 2016 - Philipe Parreiras Horta de Seixas.pdf: 1628187 bytes, checksum: d31c041a1ff12f0800060ca43a9e61f3 (MD5) / Made available in DSpace on 2017-11-09T10:53:09Z (GMT). No. of bitstreams: 1 2016 - Philipe Parreiras Horta de Seixas.pdf: 1628187 bytes, checksum: d31c041a1ff12f0800060ca43a9e61f3 (MD5) Previous issue date: 2016-08-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The purpose of the present study is to improve the techniques in the nutrition of Ucides cordatus larvae, one of the most commercially exploited resources on Brazilian?s mangroves. For such, three experiments were performed, evaluating different diets on Ucides cordatus larval development. The ovigerous females were collected by a professional crab-man, on Barra de Guaratiba mangroves, Rio de Janeiro-RJ, near the hatching period and taken to the Laborat?rio de Cultivo de Algas e Zoopl?ncton (LACAZ) of the Esta??o Experimental de Aquicultura Almirante Paulo Moreira (EEAAPM)/FIPERJ, for spawning. On experiments 1 and 2, the objective was to evaluate the replacement of the live food for a commercial formulated diet for shrimp (Larval Z Plus/Zeigler) on the development of the larvae in the zoea I stage until the megalopa (experiment 1) and from the megalopa stage to juvenile (experiment 2). On experiment 1, after 15 days, the first megalopas occurred in every treatment and the larvae survival was on average 30.1?11.8%, without significant difference between live food, mixed and commercial formulated diet treatments. Also, there was no significant difference in final survival between larvae fed with live food (13.5%), mixed (9.3%), and feed (5.5%). On experiment 2, there was no survival in the treatments whereby larvae were fed only with feed. The first juvenile occurred on the treatment with Artemia nauplii, on the 10th day. The final survival rates with Artemia treatments and mixed were of 33.3% and 8.3%, respectively. According to the chi-square test, the survival was independent of the type of food (Artemia and mixed). On experiment 3, the objetive was to evaluate Ucides cordatus larvae development from zoea I to the megalopa metamorphosis with the microalgae: Rhodomonas sp., Isochrysis galbana and Chaetoceros muelleri. The task was performed on individual cultures with 15 repetitions and semi-static system. First megalopas occurred on the 19th day in all of the treatments. The highest survival rate was observed on the treatment with Rhodomonas sp. (43%) followed by I. galbana (23%) and C. muelleri (13%), presenting significant difference (p>0,05) between only Rhodomonas sp. and C. muelleri treatements. Based on the results displayed, thereby concluded on experiment 1, despite not having been observed significant difference between the treatments, feed diet can be indicated as a more advantageous alternative on larval phases (zoea I to megalopa) by reducing risks and cost with live food production and providing more facility on management. On experiment 2, the diet exclusively with Artemia sp. was the most indicated for megalopa phase by promoting better rates of survival and a faster larval development. On experiment 3, the microalgae species Rhodamonas sp. and Isochrysis galbana were the most indicated ones for larviculture of U. cordatus, being necessary other studies in larger scale conditions in order to confirm this task results / A finalidade do presente estudo foi aprimorar as t?cnicas na alimenta??o de larvas do Ucides cordatus caranguejo-u??, um dos recursos mais explorados comercialmente nos manguezais brasileiros. Para isso, foram realizados tr?s experimentos avaliando diferentes dietas no desenvolvimento larval do caranguejo-u??. As f?meas ov?geras foram coletadas por um caranguejeiro profissional, nos manguezais de Barra de Guaratiba, Rio de Janeiro-RJ, pr?ximo ao per?odo de eclos?o, e levadas ao Laborat?rio de Cultivo de Algas e Zoopl?ncton (LACAZ) da Esta??o Experimental de Aquicultura Almirante Paulo Moreira (EEAAPM)/FIPERJ, para desova. Nos experimentos 1 e 2, o objetivo foi avaliar a substitui??o do alimento vivo por uma dieta formulada comercial para camar?o (Larval Z Plus/Zeigler) no desenvolvimento das larvas do estagio de zoea I at? megalopa (experimento 1) e do est?gio de megalopa para juvenil (experimento 2). No experimento 1, ap?s 15 dias ocorreram as primeiras megalopas em todos os tratamentos e a sobreviv?ncia de larvas durante esse per?odo foi em m?dia 30,1?11,8% sem diferen?a significativa entre os tratamentos alimento vivo, misto e dieta formulada comercial. Tamb?m n?o houve diferen?a significativa na sobreviv?ncia final entre larvas alimentadas com alimento vivo (13,5%), misto (9,3%) e ra??o (5,5%). No experimento 2, n?o houve sobreviv?ncia nos tratamentos em que as larvas foram alimentadas somente com ra??o. O primeiro juvenil ocorreu no tratamento com n?uplios de Artemia, no 10? dia. As taxas finais de sobreviv?ncia com os tratamentos Artemia e misto foram de 33,3% e 8,3% respectivamente. Segundo o teste qui-quadrado, a sobreviv?ncia foi independente do tipo de alimento (Artemia e misto). No experimento 3, o objetivo foi avaliar o desenvolvimento larval do caranguejo-u??, de zoea I at? a metamorfose para megalopa com as microalgas: Rhodomonas sp., Isochrysis galbana e Chaetoceros muelleri. O trabalho foi realizado em cultivos individuais com 15 repeti??es e sistema semi-est?tico. As primeiras megalopas ocorreram no 19? dia em todos os tratamentos. A maior taxa de sobreviv?ncia foi observada no tratamento com Rhodomonas sp. (43%) seguido da I. galbana (23%) e C. muelleri (13%), apresentando diferen?a significativa (p<0,05) somente entre os tratamentos Rhodomonas sp. e C. muelleri. Com base nos resultados apresentados, conclui-se no experimento 1, que apesar de n?o ter sido observada diferen?a significativa entre os tratamentos, a alimenta??o com ra??o pode ser indicada como uma alternativa mais vantajosa nas fases larvais (zoea I at? megalopa) por diminuir os riscos e o custo com a produ??o de alimento vivo e proporcionar maior facilidade no manejo. No experimento 2, a dieta exclusivamente com Artemia sp. foi a mais indicada para a fase de megalopa por promover melhor taxa de sobreviv?ncia e um desenvolvimento larval mais r?pido. No experimento 3, as esp?cies de microalgas Rhodomonas sp e Isochrysis galbana foram as mais indicadas para larvicultura de U. cordatus, sendo necess?rio outros estudos em escalas maiores, para confirmar os resultados deste trabalho