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<p>Det är en betydande andel svenska resenärer som idag reser till och runt i Thailand som ”backpackers”. Mitt syfte är att ta reda på om den svenska backpackerdominansen i Thailand skulle kunna betecknas som en kolonisering. Material och metod består av intervjuer och deltagande observation med sju informanter, samt fältundersökningar som bedrivits i Thailand. Slutresultatet tolkas med hjälp av den postkoloniala teoribildningen. Frågeställningarna koncentreras kring interaktionen mellan svenska backpackers och lokala subjekt, återvändandets betydelse, samt om en modern kolonisering i enlighet med Victor Mudimbes terminologi kan appliceras. Jag kommer fram till att svenska backpackers i Thailand visar tendenser och tillämpningar som tyder på en modern kolonisering av platserna de befinner sig på.</p>

<p>It is difficult for designers to explain what they do. In addition,those unfamiliar with design do not understand the rigor and logic of design thinking and process .We can’t formulate pre defined model for design process because every design situation is unique and new situation. But I tried to formulate my own model for design process as common with Inspirations from my supervisors/Lectures. I tried to describe what is design? And what is design process? In this paper.This personal position paper explores the personal improvement throughout the course work and what I learnt though out the course work.</p>

<p>Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. These reporter molecules can be designed to include tag sequences that can be analyzed by techniques for nucleic acid analysis. Herein, I present a dual-tag microarray (DTM) platform that is suitable for high-performance analyses of DNA reporter molecule libraries, generated by padlock and proximity probing reactions. The DTM platform was applied for analysis of mRNA transcripts using padlock probes, and of cytokines using proximity ligation. The platform drastically improved specificity of detection, and it allowed precise measurements of proteins and nucleic acids over wide dynamic ranges.</p><p>The thesis also presents two techniques for multi-probe analyses of biomolecules: the triple-specific proximity ligation assay (3PLA) for protein analyses, and the spliceotyping assay for mRNA analyses. 3PLA allows highly specific measurements of as little as hundreds of target protein molecules by interrogating three target epitopes simultaneously. In spliceotyping the exon composition of individual transcripts are represented as a series of tag sequences in DNA reporter molecules, via a series of target-dependent ligation reactions. Next, the splicing patterns along individual transcripts can be revealed by amplified single molecule detection and step-wise decoding.</p>

Geometric Circle Packings are of interest not only for their aesthetic appeal but also their relation to discrete analytic function theory. This thesis presents new computational methods which enable additional practical applications for circle packing geometry along with providing a new discrete analytic interpretation of the classical Schwarzian derivative and traditional univalence criterion of classical analytic function theory. To this end I present a new method of computing the maximal packing and solving the circle packing layout problem for a simplicial 2-complex along with additional geometric variants and applications. This thesis also presents a geometric discrete Schwarzian quantity whose value is associated with the classical Schwarzian derivative. Following Hille, I present a characterization of circle packings as the ratio of two linearly independent solutions of a discrete difference equation taking the discrete Schwarzian as a parameter. This characterization then gives a discrete interpretation of the classical univalence criterion of Nehari in the circle packing setting.

A new integrated layout optimization method is proposed here for the design of multi-component systems. By introducing movable components into the design domain, the components layout and the supporting structural topology are optimized simultaneously. The developed design procedure mainly consists of three parts: (i). Introduction of non-overlap constraints between components. The Finite Circle Method (FCM) is used to avoid the components overlaps and also overlaps between components and the design domain boundaries. It proceeds by approximating geometries of components and the design domain with numbers of circles. The distance constraints between the circles of different components are then imposed as non-overlap constraints. (ii). Layout optimization of the components and supporting structure. Locations and orientations of the components are assumed as geometrical design variables for the optimal placement. Topology design variables of the supporting structure are defined by the density points. Meanwhile, embedded meshing techniques are developed to take into account the finite element mesh change caused by the component movements. Moreover, to account for the complicated requirements from aerospace structural system designs, design-dependent loads related to the inertial load or the structural self-weight and the design constraint related to the system gravity center position are taken into account in the problem formulation. (iii). Consistent material interpolation scheme between element stiffness and inertial load. The common SIMP material interpolation model is improved to avoid the singularity of localized deformation due to the presence of design dependent loading when the element stiffness and the involved inertial load are weakened with the element material removal. Finally, to validate the proposed design procedure, a variety of multi-component system layout design problems are tested and solved on account of inertia loads and gravity center position constraint.

This thesis describes a new approach to biomolecular analysis, called the volume-amplified magnetic nanobead detection assay (VAM-DNA). It is a sensitive, specific magnetic bioassay that offers a potential platform for the development of low-cost, easy-to-use diagnostic devices. The VAM-NDA consists of three basic steps: biomolecular target recognition, enzymatic amplification of the probe-target complex using the rolling circle amplification (RCA) technique, and addition of target complementary probe-tagged magnetic nanobeads which exhibit Brownian relaxation behavior. Target detection is demonstrated by measuring the frequency-dependent complex magnetization of the magnetic beads. The binding of the RCA products (target DNA-sequence coils) to the bead surface causes a dramatic increase in the bead size, corresponding essentially to the size of the DNA coil (typically around one micrometer). This causes a decrease in the Brownian relaxation frequency, since it is inversely proportional to the hydrodynamic size of the beads. The concentration of the DNA coils is monitored by measuring the decrease in amplitude of the Brownian relaxation peaks of free beads. The parameters oligonucleotide surface coverage, bead concentration, bead size and RCA times were investigated in this thesis to characterize features of the assay. It was found that all of these parameters affect the outcome and efficiency of the assay. The possibility of implementing the assay on a portable, highly sensitive AC susceptometer platform was also investigated. The performance of the assay under these circumstances was compared with that using a superconducting quantum interference device (SQUID); the sensitivity of the assay was similar for both platforms. It is concluded that, the VAM-NDA opens up the possibility to perform biomolecular detection in point-of-care and outpatient settings on portable platforms similar to the one tested in this thesis. Finally, the VAM-NDA was used to detect Escherichia coli bacteria and the spores of Bacillus globigii, the non-pathogenic simulant of Bacillus anthracis. A limit of detection of at least 50 bacteria or spores was achieved. This shows that the assay has great potential for sensitive detection of biomolecules in both environmental and biomedical applications.

Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. These reporter molecules can be designed to include tag sequences that can be analyzed by techniques for nucleic acid analysis. Herein, I present a dual-tag microarray (DTM) platform that is suitable for high-performance analyses of DNA reporter molecule libraries, generated by padlock and proximity probing reactions. The DTM platform was applied for analysis of mRNA transcripts using padlock probes, and of cytokines using proximity ligation. The platform drastically improved specificity of detection, and it allowed precise measurements of proteins and nucleic acids over wide dynamic ranges. The thesis also presents two techniques for multi-probe analyses of biomolecules: the triple-specific proximity ligation assay (3PLA) for protein analyses, and the spliceotyping assay for mRNA analyses. 3PLA allows highly specific measurements of as little as hundreds of target protein molecules by interrogating three target epitopes simultaneously. In spliceotyping the exon composition of individual transcripts are represented as a series of tag sequences in DNA reporter molecules, via a series of target-dependent ligation reactions. Next, the splicing patterns along individual transcripts can be revealed by amplified single molecule detection and step-wise decoding.

It is difficult for designers to explain what they do. In addition,those unfamiliar with design do not understand the rigor and logic of design thinking and process .We can’t formulate pre defined model for design process because every design situation is unique and new situation. But I tried to formulate my own model for design process as common with Inspirations from my supervisors/Lectures. I tried to describe what is design? And what is design process? In this paper.This personal position paper explores the personal improvement throughout the course work and what I learnt though out the course work.

Det är en betydande andel svenska resenärer som idag reser till och runt i Thailand som ”backpackers”. Mitt syfte är att ta reda på om den svenska backpackerdominansen i Thailand skulle kunna betecknas som en kolonisering. Material och metod består av intervjuer och deltagande observation med sju informanter, samt fältundersökningar som bedrivits i Thailand. Slutresultatet tolkas med hjälp av den postkoloniala teoribildningen. Frågeställningarna koncentreras kring interaktionen mellan svenska backpackers och lokala subjekt, återvändandets betydelse, samt om en modern kolonisering i enlighet med Victor Mudimbes terminologi kan appliceras. Jag kommer fram till att svenska backpackers i Thailand visar tendenser och tillämpningar som tyder på en modern kolonisering av platserna de befinner sig på.