MSc., Faculty of Science, University of the Witwatersrand, 2011 / A hierarchical non-linear model was developed for the time-dependent shrinkage behaviour of South African concretes, from historical laboratory data. The fit of fifteen growth curve models to the shrinkage-time profiles was evaluated and MCDA was used to identify the best model. The three parameters of the chosen growth curve model were modelled in terms of covariates (relating to concrete raw materials, concrete composition and shrinkage testing conditions) by multivariate multiple regression to produce the WITS model. The model largely conformed to existing knowledge about the factors affecting concrete shrinkage. Published models for concrete shrinkage were compared to the WITS model regarding their predictive ability with respect to the South African data set. The WITS model performed the best across a variety of graphical and numerical goodness-of-fit measures. The importance of the study is two-fold:  The concept of hierarchical non-linear modelling has been applied for the first time to the modelling of the time-dependent properties of concrete.  This is the first comprehensive model to bring together laboratory data on the shrinkage of concrete generated in South Africa over a span of thirty years.

Os equipamentos fotoativadores apresentam diferentes características e elas podem influenciar na polimerização de restaurações em resina composta. O objetivo desse estudo foi avaliar as características de quatro equipamentos fotoativadores e verificar sua influência na polimerização de duas resinas compostas bulk fill. Os equipamentos fotoativadores Bluephase 20i (Ivoclar Vivadent), Celalux 3 (Voco), Elipar DeepCure-S (3M ESPE) e Valo Grand (Ultradent) foram avaliados quanto ao diâmetro da ponteira, à potência, à irradiância, ao espectro de emissão e ao perfil do feixe de luz, e utilizados na fotoativação das resinas compostas Filtek Bulk Fill Posterior Restorative - A2 (3M ESPE) e Tetric EvoCeram Bulk Fill - IVA (Ivoclar Vivadent). Matrizes de Delrin® brancas foram utilizadas para confecção de espécimes de 12 mm de diâmetro e 4 mm de espessura, sendo a dureza nas superfícies de topo e fundo desses espécimes avaliadas com 97 endentações por superfície, no centro e em todas as direções. Uma matriz de dente foi confeccionada para a avaliação da dureza transversal de restaurações de 12 mm de largura e 4 mm de profundidade, sendo feitas endentações no centro e nas regiões mesial e distal (4,5 mm do centro), a cada 0,5 mm de profundidade. Após os ensaios de microdureza, os espécimes foram imersos em Tetrahidrofurano por 24 horas. A transmissão de luz através dos espécimes também foi avaliada. Os dados obtidos foram analisados estatisticamente com testes de ANOVA e Tukey, com nível de significância de 5%, e teste t. Os valores de dureza mensurados nos espécimes confeccionados com auxilio das matrizes de Delrin® mostram que os fatores resina composta e superfície mensurada exercem influência sobre os resultados (p<0,05). A interação entre o fator equipamento fotoativador e a região das endentações também influenciou os resultados, sendo que o uso do Valo Grand não resultou em diferenças estatisticamente significantes entre os valores mensurados no centro e na região externa (p>=0,05). Quando o Celalux 3 foi utilizado, houve diferença estatisticamente significante entre os valores obtidos nas regiões central e externa (p<0,05). Os resultados de dureza mensurados nos espécimes confeccionados com a matriz feita com uso de dente humano mostraram diferenças estatisticamente significantes entre 10 e 20 segundos de fotoativação (p<0,05). O fator resina composta também exerceu influência sobre os resultados. Houve diferença estatisticamente significante entre o centro e as regiões mesial e distal quando todos os equipamentos fotoativadores foram utilizados (p<0,05). Também foi observada diferença estatisticamente significante entre os valores obtidos nas caixas mesial e distal dos espécimes (p<0,05). Os resultados de transmissão de luz mostraram que pouca luz violeta chega ao fundo dos espécimes e a posição e o tamanho da ponteira dos equipamentos fotoativadores influenciam na irradiância da luz que chega no fundo das caixas proximais de restaurações MOD. Os equipamentos fotoativadores apresentaram diferenças, sendo que elas, associadas à diferentes protocolos de fotoativação, influenciaram na dureza de restaurações confeccionadas com resinas compostas bulk fill. / Light curing units can present different characteristics and they can influence the polymerization of composite resin restorations. The aim of this study was to analyze the characteristics of four light curing units and verify their influence on the polymerization of two bulk fill composite resins. The light curing units Bluephase 20i (Ivoclar Vivadent), Celalux 3 (Voco), Elipar DeepCure-S (3M ESPE) and Valo Grand (Ultradent) were analyzed regarding their tip diameter, radiant power, irradiance, emission spectrum and light beam profile, and used for the photoactivation of the composite resins Filtek Bulk Fill Posterior Restorative - A2 (3M ESPE) and Tetric EvoCeram Bulk Fill - IVA (Ivoclar Vivadent). White Delrin® matrices were used for the confection of 12 mm diameter and 4 mm depth specimens, being their hardness measured at the top and bottom surfaces, with 97 indents per surface, at the center and all the directions. One matrix, made using human tooth, was used for the confection of 12mm wide and 4 mm depth restorations, in which the transversal hardness was measured. Indents were made at the center and the mesial and distal regions (4,5 mm from the center) of the restorations, at every 0,5 mm in depth. After the microhardness tests, the specimens were immersed in Tetrahydrofuran for 24 hours. The light transmission through the specimens was also analyzed. The obtained data was statistically analyzed with the ANOVA and Tukey tests, with a 5% significance level, and t test. The hardness values measured at the specimen made with the Delrin® matrices showed that the factors composite resin and surface influenced the results (p<0,05). The interaction between the light curing unit and the region of the indents also influenced the results, when the Valo Grand was used, there was no statistically significant difference between center and the external regions (p>=0,05). When the Celalux 3 was used, there was a statistically significant difference between the central and external regions (p<0,05). The hardness results obtained with the specimen made using the matrix of human tooth showed statistically significant differences between the 10 and 20 second photoactivation times (p<0,05). The factor composite resin also influenced the results. There was a statistically significant difference between the center and the mesial and distal regions when all the light curing units were used (p<0,05). There was also a statistically significant difference between the values measured on the mesial and distal boxes of the specimen (p<0,05). The light transmission results showed that little violet light reaches the bottom of the specimens and the position and the light curing unit\'s tip diameters influence the irradiance of light that reaches the bottom of the proximal boxes of MOD restorations. The light curing units showed different characteristics and they, associated to different photoativation protocols, influence the hardness of bulk fill composite resin restorations.

Este trabalho avaliou in vitro a dureza e módulo de elasticidade de uma resina composta nanohíbrida (Tetric N-Ceram/Ivoclair-Vivadent) com diferentes cores, fotoativada por duas unidades com diferentes comprimentos de onda em 3 espessuras (1, 2 e 3mm) comparadas à partir da superfície irradiada (0mm). Os espécimes foram divididos em grupos (n=5): microdureza Knoop-KHN, ultramicrodureza-DUH e módulo de elasticidade-ME, cor (A2 e Bleach-M(BM), fotoativador LEDazul (Elipar Freeligth2/3M(750mW/25s/430-480nm(FL) e LEDazul/violeta Bluephase/Ivoclar/Vivadent (1200mW/cm²/15s/380-515nm(B15) e 30s(B30). Os espécimes foram armazenados por 24h/37ºC. Os ensaios de KHN foram realizados no HMV-2000/Shimadzu, com carga de 25gf/40s, e de DUH e ME no DUH 211S/Shimadzu com força de 10mN e tempo de espera de 0s. Foram realizadas 5 endentações na superfície irradiada (0mm) e na base das amostras (1, 2 e 3mm). A análise estatística foi realizada por ANOVA, Tukey e correlação de Pearson (p<0,01%). O teste de Pearson mostrou haver correlação direta entre a KHN e a DUH. A fonte FL promoveu maiores valores de KHN e de DUH para as duas resinas testadas; enquanto que a fonte B15, promoveu o maior valor de ME. Quanto às espessuras, 0 e 1mm apresentaram maiores valores de KHN e de DUH, e menores de ME que as espessuras de 2 e 3mm. A cor A2 apresentou maior valor de KHN e DUH e menor ME que a cor Bleach-M. Assim, de acordo com a metodologia proposta e os resultados apresentados pode-se concluir que apesar de a luz LEDazul/violeta propor uma maior eficácia na polimerização de resinas de cor clara, no tocante às propriedades avaliadas isso não foi verificado. / This study evaluated in vitro the hardness (micro and ultramicro) and elastic modulus of a nanohybrid composite resin (Tetric N-Ceram/Ivoclar-Vivadent) with different colors, light sources and thickness. The specimens were divided into groups (n=5): KHN-Knoop microhardness, DUH-nanohardness and ME-elastic modulus; colors: A2 and Bleach-M (BM); light sources: LEDblue Elipar Free light 2/3M-ESPE (750mW/cm²/ 25s/430-480nm- (FL)), LEDblue/violet Bluephase/Ivoclar-Vivadent (1.200mw/cm²/15s/380-515nm) (B15) and 30s (B30); thickness: 1mm, 2mm and 3mm, compared to the irradiated surface (0mm). Specimens were stored dry for 24hours at 37ºC after photo curing. Assays were performed at KHN in HMV-2000/ Shimadzu with load of 25gf at 40s and DUH/ME in DUH 211S/Shimadzu with force of 10mN and no hold time (0s). 5 indentations of each test were made in the surfaces opposites to the photo curing. A statistical analysis was performed by ANOVA, Tukey and Pearson correlation (p<0.01%). Pearsons Test showed a direct correlation between KHN and DUH. Comparison between KHN and ME in the studied resins indicated that the light source FL promoted higher values of KHN and DUH to both resins tested, whereas the B15 light source promoted the higher ME value. Regarding thickness 0mm and 1mm showed higher KHN and DUH and lower ME than 2 and 3mm thickness. A2 color showed higher KHN and DUH and lower ME than BM. Thus, according to the proposed methods and the presented results, the LEDblue/violet was not better for curing the light color of the nanohybrid resin, than LEDblue.

Nas edificações, as argamassas de revestimento têm diversas funções e sabe-se que o perfeito desempenho daquelas está diretamente relacionado com o tipo de argamassa empregada, suas propriedades tanto no estado fresco quanto no endurecido, como também das diversas propriedades do substrato no qual a mesma será aplicada. O baixo desempenho de uma ou mais destas funções pode gerar o aparecimento de manifestações patológicas, fatores que fazem com que as argamassas deixem de cumprir suas funções trazendo desconforto ao usuário bem como custos de manutenção e reparo. A temperatura e a umidade relativa do ar, durante a cura do revestimento, influenciam a velocidade de endurecimento da argamassa e sabe-se que além temperaturas com grande amplitude de variação ao longo do dia. Com base neste contexto, este trabalho busca avaliar o desempenho de argamassas de revestimento (industrializada, preparada em obra com utilização de cal hidratada e preparada em obra com utilização de aditivo incorporador de ar) quando aplicadas a substratos cerâmicos com 3 níveis de absorção de água, e curadas nas temperaturas de 5, 20 e 40°C. Para tal, verificou-se o comportamento em termos de resistência de aderência à tração do revestimento, além de análises realizadas em corpos-de-prova para cada tipo de argamassa de revestimento. Em relação à temperatura de cura, pode-se dizer que a temperatura intermediária (20°C) apresentou melhores resultados para a grande maioria das combinações entre argamassas de revestimentos e blocos cerâmicos. No que se refere à argamassa preparada em obra com a utilização de cal hidratada, o substrato com menor absorção de água, com cura em temperatura intermediária, apresentou melhor aderência. / In constructions, mortars has many functions and it’s known that these perfect performance are directly related with the mortar kind, the properties in fresh and hardened stages, as also of the bond properties in which it will be applied. Overhead of one or more of these functions can accelerate the pathological manifestations appearance, things that make that mortars leaves off these functions bringing the user discomfort as well as maintenance costs and repair. The temperature and the air relative humidity, during the cure has influence in the mortar hardening. Therefore it´s true that all day long we have beyond temperatures with great variation. In this context, this dissertation searches to evaluate the mortars performance (industrialized, prepared with lime and prepared with additive) when applied in bricks with 3 water absorption levels, and cured temperatures of 5, 20 and 40°C. For such, the traction strength behavior was verified, beyond analyses in samples for each mortar kind. About the cure temperature, it can be said that the medium temperature (20°C) presented better resulted for the great majority of the combinations between mortars and ceramic bricks. About mortars, the one prepared with lime, the bond with lower water absorption, with medium cure temperature, presented better performance.

La mise en place de la démarche PAT (Process Analytical Technology), initiée par la FDA (Food and Drug Administration) s’est développée au cours de ces dernières années, au sein de l’industrie pharmaceutique. Grâce à des contrôles en continu au coeur des procédés de fabrication, elle permet une meilleure compréhension et une maîtrise de la formulation et du procédé, afin d’assurer la qualité finale des médicaments.A travers ce travail, nous avons mis en place un suivi en temps réel, par spectroscopie proche infrarouge, d’une opération d’enrobage suite à l’intégration d’une sonde à l’intérieur d’une turbine d’enrobage. La quantité d’enrobage, déterminée par une simple et rapide pesée mais néanmoins soumise à la variabilité de la masse des comprimés nus, ainsi que l’épaisseur du film, obtenue avec précision par imagerie térahertz ont servi de valeurs de référence pour calibrer l’information spectrale. Dans les deux cas, ces deux attributs qualité critiques ont été prédits avec de faibles erreurs de prédiction, qui se sont révélées être similaires. Par ailleurs, la prédiction en temps réel des propriétés de dissolution de comprimés prêts à être libérés, à partir de spectres acquis in-line, a permis de déterminer l’arrêt optimal de l’opération d’enrobage.Suite à un enrobage réalisé à partir d’une dispersion aqueuse de polymère une étape supplémentaire de traitement thermique ou curing est généralement nécessaire afin de stabiliser le film d’enrobage. Un travail de caractérisation mené à partir de techniques innovantes a permis d’apporter un nouvel éclairage sur la compréhension des phénomènes impliqués dans la formation du film au cours du curing. La caractérisation approfondie de la structure d’enrobage de comprimés soumis à un curing en turbine (conditions dynamiques) a mis en évidence la diminution de la porosité, couplée à l’évaporation de l’eau et à une meilleure organisation des chaînes de polymère au cours du curing. L’étude de comprimés soumis à un curing de référence en étuve durant 24 h (conditions statiques) a confirmé l’obtention d’un film stable après 4 h de curing dynamique. De nouveaux phénomènes, indépendants du curing, liés à la cristallisation et à la migration de l’alcool cétylique, couplée à la migration du lauryl sulfate de sodium, au sein de la couche d’enrobage ont été détectés au cours de la conservation des comprimés enrobés. / Implementation of PAT (Process Analytical Technology) approach has recently been promoted by the FDA (Food and Drug Administration) within the pharmaceutical industry. A desired goal of the PAT framework is to enhance understanding and control of the manufacturing process through timely measurements, during processing, to ensure final product quality. Real-time monitoring of a coating operation was performed from in-line Near Infrared (NIR) measurements inside a pan coater. Mass of coating materials, determined by simple and fast weighing but depending on core tablet weight uniformity, and film coating thickness, obtained from accurate and non-destructive Terahertz Pulsed Imaging (TPI) measurements, were used as reference values to calibrate NIR spectral information. In both cases, these two critical quality attributes were predicted with low predictive errors, which were found to be similar. In addition, real-time predictions of drug release from cured tablets were carried out by in-line NIR measurements. The coating operation was successfully stopped when desired dissolution criteria were achieved. A post-coating thermal treatment, known as curing, is generally required to stabilize film coating from aqueous polymer dispersion. Innovative techniques were jointly used to elucidate the underlying mechanisms of film formation along the curing process. This study provided a new insight into the tablet coating structure, highlighting a reduced internal coating porosity, a decrease in water content and showing a better structural rearrangement of polymer chains, with dynamic curing. All investigated techniques confirmed that a stabilized state was reached after a 4 h dynamic curing in comparison with a reference curing carried out in an oven for 24 h. Interestingly even prior to curing, new findings were pointed out, during coated tablets storage, related to the crystallisation and the upward migration of cetyl alcohol, coupled to the downward migration of sodium lauryl sulfate within the coating layer.

In today’s world the significance of bio-based materials are increasing rapidly because ofthe environmental concern. Material scientists are nowadays engaged in development ofsuch materials which have natural origin and degrade in its environment. Several workshave already been reported in area of thermoplastic biocomposites. However biocompositesbased on thermosets is comparatively new area of research. In this work biobasedcomposites have been developed from two different bio-based thermoset resins.The main objective of the work was room temperature curing of poly lactic acid basedresin (POLLIT™) and AESO, acrylated epoxidized soy-bean oil (TRIBEST®). These tworesin systems were impregnated with different natural fibre mats. Cure behavior wascharacterized by means of DSC (Differential Scanning Calorimeter) and results showsthat the resins have been cured at room temperature. The mechanical properties ofprepared composites were assessed by the means of flexural testing and charpy impacttesting. The viability of using these composites in structural applications are also beendiscussed. / Uppsatsnivå: D

Thesis (Elec.E)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1982. / MICROFICHE COPY AVAILABLE IN ARCHIVES AND ENGINEERING / Includes bibliographical references. / by Huan Lim Lee. / Elec.E

Submitted by PPG Engenharia e Tecnologia de Materiais (engenharia.pg.materiais@pucrs.br) on 2018-10-11T17:16:47Z No. of bitstreams: 1 Disserta??o Rafael Bitello Silva.pdf: 3894988 bytes, checksum: cf3a5e12050e0f5509ae1930f1b89181 (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-10-17T12:16:39Z (GMT) No. of bitstreams: 1 Disserta??o Rafael Bitello Silva.pdf: 3894988 bytes, checksum: cf3a5e12050e0f5509ae1930f1b89181 (MD5) / Made available in DSpace on 2018-10-17T12:23:40Z (GMT). No. of bitstreams: 1 Disserta??o Rafael Bitello Silva.pdf: 3894988 bytes, checksum: cf3a5e12050e0f5509ae1930f1b89181 (MD5) Previous issue date: 2018-07-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / In this study the influence of different commercial curing agents of epoxy resin on film capacitors encapsulation was evaluated. A formulation with DGEBA epoxy resin was used with three different curing agent types: anhydride base, aliphatic amine base and polyamidoamine base. The curing reaction kinetic analysis of each epoxy system was performed by Osawa dynamic method by DSC according ASTM E968. The thermal properties of the epoxy systems were checked by TGA and DSC while the mechanical properties were measured by Shore D hardness and IZOD impact strength, also the a characterization by SEM was performed. The epoxy systems performance in capacitor was verified by qualification tests for film capacitors for automotive application based in AEC-Q200 D, as: exposure to the maximum capacitor operating temperture, exposure to thermal cycles and exposure to humidity. The capacitors were characterized by the capacitance variation while the effects in the epoxy systems were checked by their thermal and mechanical properties change. The systems based on aliphatic amine and polyamidoamine had lower activation energy and higher reaction velocity than the anhydride system. The performance of the capacitor against humidity was similar between the evaluated systems showing low loss of capacitance after the test, whereas against thermal cycles, even the aliphatic amine and polyamidoamine base systems were not affected by reduction of mechanical properties, they had their performance affected by cracks arising. Regarding the performance under exposure to the maximum operating temperature, the anhydride system presented the lowest capacitance loss in the capacitor, followed by the samples with polyamidoamine and aliphatic amine, respectively, which showed a significant reduction of the mechanical properties after prolonged exposure to the temperature of the test. / Neste estudo avaliou-se a influ?ncia de diferentes agentes de cura comerciais de resina ep?xi no encapsulamento de capacitores de filme. Foi utilizado uma formula??o de resina ep?xi DGEBA (diglicidil ?ter de bisfenol-A) com tr?s tipos diferentes de agente de cura: base anidrido, base amina alif?tica e base poliamidoamina. A an?lise da cin?tica da rea??o de cura de cada sistema ep?xi foi realizada pelo m?todo din?mico de Osawa, por DSC conforme ASTM E968. As propriedades t?rmicas dos sistemas ep?xi foram determinadas por TGA e DSC enquanto que as propriedades mec?nicas foram medidas atrav?s de dureza Shore D e resist?ncia ao impacto IZOD, al?m disto foi realizado caracteriza??o por MEV. O desempenho dos sistemas ep?xi no capacitor foi verificado pelos ensaios de qualifica??o de capacitores de filme para aplica??o automotiva baseados na norma AEC-Q200 D, sendo estes: exposi??o a temperatura m?xima do capacitor, exposi??o a ciclos t?rmicos e exposi??o a umidade. Os capacitores foram caracterizados pela varia??o de capacit?ncia enquanto que os efeitos nos sistemas ep?xi foram acompanhados pela altera??o em suas propriedades t?rmicas e mec?nicas. Os sistemas a base de amina alif?tica e poliamidoamina apresentaram menor energia de ativa??o e maior velocidade de rea??o que o sistema anidrido. O desempenho do capacitor frente a umidade foi similar entre os sistemas avaliados com baixa perda de capacit?ncia ap?s o ensaio, enquanto que frente aos ciclos t?rmicos, mesmo n?o apresentando redu??o de propriedades mec?nicas os sistemas base amina alif?tica e poliamidoamina tiveram seu desempenho afetado pelo aparecimento de trincas. Em rela??o ao desempenho sob exposi??o ? temperatura m?xima o sistema anidrido foi o que apresentou menor perda de capacit?ncia no capacitor, seguido das amostras com poliamidoamina e amina alif?tica, respectivamente, sendo que estas apresentaram redu??o significativa das propriedades mec?nicas ap?s exposi??o prolongada a temperatura do ensaio.

O aumento da vida útil de pneus tem sido uma incessante busca de seus fabricantes. Aumentar a vida de um pneu não significa somente prolongar sua vida, mas também manter estáveis as características e propriedades, para as quais os compostos foram projetados. Para manter estáveis as propriedades e características dos compostos, deve-se ter a estabilidade das ligações cruzadas formadas durante a vulcanização do pneu. Em condições normais de uso, os principais causadores da degradação das ligações cruzadas em compostos de pneus são os ataques térmicos e oxidativos. O objetivo deste trabalho é avaliar a estabilidade de um composto de borracha natural frente às solicitações térmica e oxidativa das ligações cruzadas formadas sob diferentes condições de vulcanização e sistemas vulcanizantes. Foram elaboradas cinco formulações baseadas em borracha natural do tipo RSS-3 e com variações na dosagem de enxofre e óxido de zinco, utilizando sistemas de vulcanização convencional e eficiente. Todos os compostos foram vulcanizados em duas condições, 170°C e 140°C. Os compostos vulcanizados foram avaliados em ensaios físicos e químicos, incluindo medições de módulos de armazenamento e de perda, tensão e alongamentos à ruptura, resistência à laceração, densidade de ligações cruzadas e cinética de vulcanização nas condições novo (original) e envelhecidos na presença e na ausência de oxigênio. Os resultados obtidos permitem afirmar que a utilização de oxido de zinco de alta área superficial e de sistemas de vulcanização eficientes tornam este tipo de composto mais resistentes ao envelhecimento térmico e oxidativo. / The increment of the service life of tires has been a constant challenge for their manufacturers. Increasing the life of a tire does not mean only prolong his life, but also maintain its characteristics and properties stable, whatever the compounds were designed for. To keep stable the properties and characteristics of the compounds, we must have the stability of the crosslinks formed during the vulcanization of the tire. In normal use, the main causes of the degradation of the crosslinks are due to the thermal and oxidative attacks. The objective of this study is to evaluate the thermal and oxidative stability of the crosslinks formed under different conditions and curing systems in a compound with natural rubber. Five formulations were prepared based on the natural rubber RSS-3 and variations in the dosage of sulfur and zinc oxide, as well as the type of the vulcanization system, conventional or efficient, were introduced. All compounds were cured in two conditions, 170°C and 140°C. The vulcanized compounds were evaluated by physical and chemical tests, including measurements of the storage and loss moduli, tension and stretching to break, tear resistance, crosslinking density and kinetics of curing. The performance of the compounds was evaluated under three conditions, namely new (original) and after aging in the presence and in the absence of oxygen. The results allowed us to conclude that the use of zinc oxide of high surface area and the efficient vulcanization systems are more resistant to thermal and oxidative aging.

The word "Prion" refers to self-perpetuating protein aggregates that cause neurodegenerative diseases in mammals. It is a protein isoform that has undergone a conformational change which converts the normal form of the protein into the infectious form with the same amino acid sequence. Yeast [PSI+] prion is the prion isoform of Sup35 protein, a translation termination factor eRF3. It has been suggested that prion [PSI+] is controlled by the ensemble of chaperones with Hsp104 playing the major role. The previous work performed in the Chernoffs lab showed that the defective GET pathway caused by get led to the defect in [PSI+] curing by excess Hsp104. The GET pathway is a system responsible for transporting newly synthesized TA-protein to the ER membrane, and the components which have been proven to be involved in this pathway include: Get1, Get2, Get3, Get4, Get5 and Sgt2. In this study we describe the mechanism underlying the effect of the defective GET pathway on [PSI+]. We demonstrate that Sgt2, one of the components of GET pathway, interacts with Sup35 in both [PSI+] and [psi-] strains through its prion domain. Overproduction of Sgt2 and Hsp70-Ssa is triggered by the defective GET pathway and leads to the protection of [PSI+] aggregates from curing by excess Hsp104. We show that the direct interaction between Sgt2 and Hsp70-Ssa is not required for this protective effect.