Introdução: A comunicação interatrial tipo ostium secundum(CIA) é uma cardiopatia congênita frequente, sendo a mais comumente encontrada na população adulta. Seu tratamento está indicado quando há repercussão hemodinâmica caracterizada pelo aumento das dimensões das câmaras direitas à ecocardiografia, independente da presença de sintomas. Nas últimas 2 décadas o fechamento percutâneo da CIA emergiu como a modalidade terapêutica preferencial devido sua alta eficácia e menor morbidade que a correção cirúrgica.Tanto o tratamento cirúrgico como o percutâneo da CIA resultam em remodelamento cardíaco com redução progressiva do tamanho das câmaras direitas e aumento das esquerdas. Recentemente, novas técnicas ecocardiográficas vem sendo empregadas para avaliação das dimensões, geometria e função das câmaras cardíacas incluindo o ecocardiograma tridimensional e o rastreamento de marcadores acústicos. Hipótese e objetivos: Partiu-se da hipótese que o fechamento percutâneo da CIA, por ser um método não invasivo, levaria a rápido remodelamento cardíaco mesmo em adultos com sobrecarga volumétrica crônica das câmaras direitas. Tivemos como objetivo avaliar o comportamento temporal do remodelamento cardíaco e analisar possíveis diferenças existentes entre pacientes de diferentes faixas etárias e com tamanhos diversos de CIA. Material e métodos: Estudo observacional, prospectivo, não randomizado de um braço único de uma coorte de adultos submetidos ao fechamento percutâneo da CIA com a prótese Cera (Lifetech, Shenzheng, China) e acompanhados por um ano. Foram selecionados 29 adultos com CIA com repercussão hemodinâmica com anatomia favorável para a oclusão percutânea e sem contra-indicações para tal. O remodelamento cardíaco foi estudado por meio de várias técnicas ecocardiográficas incluindo as clássicas e outras de introdução recente. A ecocardiografia bidimensional foi usada para a medição das dimensões do átrio direito (AD), ventrículo direito (VD) e ventrículo esquerdo (VE), para determinação da área fracionada do VD (FAC) e da excursão anterior da valva tricúspide (TAPSE) e para análise volumétrica das câmaras cardíacas. A ecocardiografia tridimensional foi empregada para análise volumétrica e funcional do VD. O rastreamento de marcadores acústico foi utilizado para avaliação da função do AD, VD e VE. O teste ANOVA foi usado para avaliação das mudanças observadas nas variáveis repetidas ao longo do tempo com comparações múltiplas de Bonferroni quando aplicável. Uma análise intra e interobservador das medidas foi realizada utilizando coeficientes de concordância. Resultados: A média de idade e peso dos pacientes foi de 45,2 ± 17,0 anos e 68,8 ± 14,0 kgs, respectivamente. Nenhum paciente apresentada hipertensão pulmonar significativa. A média do tamanho da CIA foi de 20,2 ± 5,0 mm e a média do tamanho da prótese implantada foi de 22,9 ± 6,2 mm. Em todos os pacientes houve sucesso no implante. Não houve complicações relacionadas ao procedimento e em todos os pacientes foi observada oclusão do defeito. Após o procedimento, houve redução precoce (< 3 meses) das dimensões do AD (p<0,001) e do VD (p<0,001) e aumento das dimensões do VE (p<0,014). Não houve mudanças significativas na função de deformação longitudinal do AD para onda P (p=0,227) e para onda T (p=0,124). Houve redução abrupta da função do VD pelo TAPSE (p=0,032), pela deformação longitudinal (p=0,002) e pela ecocardiografia tridimensional (p=0,084). Não houve mudanças significativas da onda S\' (p=0,55) e da FAC (p=0789) do VD. Houve redução precoce do volume de ejeção do VD (p< 0,001) e aumento do volume de ejeção do VE (p=0,027). Houve redução da deformação longitudinal do VE (p=0,049) e não houve mudanças na função do VE pelo método de Simpson (p=0,462). Pacientes maiores que 60 anos (n=8) apresentaram valores iniciais maiores nas dimensões do AD e redução mais prolongada (p=0,0497). Pacientes com CIAs maiores que 20 mm (n=12) apresentavam valores iniciais maiores de TAPSE com redução mais retardada (p=0,013). Todas as mudanças observadas inicialmente nos primeiros 3 meses se sustentaram ao final do seguimento. Houve excelente concordância na análise intraobservador para todas as medidas repetidas (CCI> 0,9) com exceção da deformação do VE (CCI< 0,7). A concordância entre as medidas realizadas por diferentes observadores não foi tão boa, com apenas as variáveis TAPSE, deformação do VE e dimensões do AD possuindo CCI > 0,7. Conclusões: O fechamento percutâneo da CIA em adultos de meia idade leva a rápido remodelamento cardíaco tanto do ponto de vista anatômico como funcional com resultados sustentados dentro do primeiro ano de seguimento. Tais mudanças temporais são observadas independente do tamanho da CIA e da idade dos pacientes, denotando o efeito benéfico do procedimento sobre a geometria e o funcionamento cardíaco global em todos adultos portadores desta frequente cardiopatia congênita. / Introduction: The atrial septal defect of the secundum type (ASD) is a frequent congenital heart disease, being the most commonly encountered in the adult population. Treatment is indicated when there is hemodynamic burden characterized by increased dimensions of the right chambers on echocardiography, regardless of the presence of symptoms. In the last two decades, percutaneous closure of the ASD has emerged as the preferred therapeutic modality due to its high efficacy and lower morbidity when compared to surgical correction. Both surgical and percutaneous treatment of the ASD result in cardiac remodeling with progressive reduction in the size of the right chambers and increase in the left chambers. Recently, new echocardiographic techniques have been employed to assess the dimensions, geometry and function of the cardiac chambers including three-dimensional echocardiography (3D echo) and acoustic speckle tracking. Hypothesis and objectives: Hypothesizing that percutaneous closure, being a non-ivasive method, results in a fast cardiac remodeling even in adults with chronic volume overload of the right chambers, our aim was to assess the temporal pattern of cardiac remodeling and analyse possible differences between patients of different age groups and different ASD sizes. Material and methods: This was an observational, prospective, non randomized, single arm study of a cohort of adults submitted to percutaneous closure of the ASD with the Cera device (Lifetech, Shenzheng, China) followed along a year. Twenty nine adults with hemodynamicaly significant ASDs with suitable anatomy for percutaneous closure and no contra-indications for the procerdure were selected. Cardiac remodeling was assessed by various echocardiographic techniques including standard and new ones. Bidimensional echocardiography was used to measure the dimensions of the right atrium (RA), right ventricle (RV) and left ventricle (LV), to determine the fractional area of the RV (FAC) and the systolic anterior excursion of the tricuspid valve (TAPSE), and to analyse the volumes of the cardiac chambers. Three-D echo was employed for volumetric and functional analysis of the RV. Acoustic speckle tracking was utilized to assess the function of the RA, RV and LV. ANOVA tests were used to assess the observed changes in the repeated variables over time with multiple Boferoni comparison as applicable. An intra and interobserver analysis of the measurements was performed using concordance coefficients. Results: Mean age and weight was 45,2 ± 17,0 years and 68,8 ± 14,0 kgs, respectively. No patient had significant pulmonar arterial hypertension. The ASD dimension and the size of the device was a mean of 20,2 ± 5,0 mm and 22,9 ± 6,2 mm, respectively. In all patients the device was implanted successfully. There were no complications related to the procedure and in all patients the ASDs were successfully closed. After the procedure, there was an early (< 3 months) reduction of the RA (< 0.001) and RV (< 0.001) sizes and an increase of the LV dimensions (p< 0.014). There were no significant changes in the function of the RA as assessed by longitudinal strain (p=0.227 for the P wave and p=0.124 for the T wave). There was an abrupt reduction of the RV function assessed by TAPSE (p=0.032), longitudinal strain (p=0.002) and 3D echo (p=0.084). There were no changes in the S\' wave (p=0.55) and FAC (p=0.789) of the RV. There was an immediate decrease in the RV stroke volume (p< 0.001) and an increase in the LV srtroke volume (p=0.027). There was a reduction in LV longitudinal strain (p=0.049) and no change in LV function as assessed by the Simpson method (p=0.462). Patients older than 60 years of age (n=8) presented with larger RA dimensions, which decreased in a slower fashion (p=0.0497). Patients with ASDs larger than 20 mm (n=12) had initial higher TAPSE values, which decreased in a slower fashion (p=0.013). All changes observed earlier on endured after a year. There was excellent concordance in the intra observer analysis for all the repeated measures (CCI > 0.9) with the exception of LV strain (CCI < 0.7). The concordance between different observers was not as good with only TAPSE, LV strain, and RA dimensions variables having a CCI > 0.7. Conclusions: Percutaneous closure of the ASD in middle aged adults results in fast cardiac remodeling from both the anatomic and functional point of view with sustained results over the first year of follow up. These temporal changes are observed regardless of the ASD size and the age of the patients, which demonstrates the beneficial effect of the procedure over the cardiac geometry and global function in all adults who have this frequent congenital heart disease.

Defeitos de cartilagem e a mais comum doença articular, a osteoartrite, são caracterizadas pela destruição da cartilagem articular, e consequentemente na perda da função articular em humanos e animais. As estratégias atuais de tratamento, conservativas e cirúrgicas, são insuficientes: não resultam em restauração total da cartilagem hialina, e, portanto trazem um prognóstico reservado a longo prazo. O presente estudo tem por objetivo avaliar os efeitos do administração conjunta de concentrado de medula óssea, plasma rico em plaqueta sobre lesões condrais experimentalmente induzidas e tratadas com microfraturas e ácido hialuronico. Foram utilizadas as articulações metacarpofalangeana de 6 éguas, as quais foram divididas em 2 grupos aleatoriamente e cego. Foram feitos defeitos condrais totais através de artroscopia e, todos foram tratados com microfraturas e ácido hialurônico no transoperatório (M 0) sendo, esse repetido após 15 dias (M 15) e 30 dias (M 30). Grupo C (controle) e grupo T (tratado). O grupo T foi tratado com aspirado concentrado de células tronco de medula óssea adicionada ao plasma rico em plaquetas (PRP), os quais foram injetados na articulação no final da cirurgia (M0). O grupo T recebeu ainda 2 aplicações articulares adicionais de PRP em 15 dias (M 15) e 30 dias (M 30). As seguintes avaliações foram realizadas: exame clínico de claudicação, ultrassonografia, estudo radiográfico, avaliações de líquido sinovial (físico, bioquímico e citológico). As avaliações foram realizadas antes da cirurgia (M 0), com 3, 5 e 7 dias. Posteriormente a cada 15 dias (M 15, M 30, M 45 e M 60) e os 3 últimos momentos foram aos 90 (M90), 120 (M120) e 210 (M210) dias. Ao final do experimento os animais foram enviados para abate comercial. Foram verificadas diferenças estatísticas (p<0,05) entre o grupo tratado e controle para avaliação de proteínas no líquido sinovial corrigido por uréia em 3, 5 e 7 dias; para PGE2 no líquido sinovial em 3 e 5 dias onde para ambas as variáveis com maiores valores para o grupo tratado. Já a concentração de ácido hialurônico apresentou maiores valores (p<0,05) em 3, 45 e 90 dias no grupo controle. Para as demais variáveis não houve diferença estatística entre o grupo tratado e controle. Porém, notou-se medianas maiores para condroitin sulfato em 3 e 5 dias no grupo controle. Notavelmente, o grupo tratado apresentou melhor escore macroscópico na avaliação do tecido de reparo. Contudo, a administração intra-articular de concentrado de medula óssea e plasma rico em plaquetas sobre lesões condrais induzidas e tratadas com microfraturas e ácido hialurônico produziu uma reação articular transitória, principalmente nos primeiros 60 dias, e foi evidenciado pelo aumento de PGE2 e proteínas no líquido sinovial, bem como, claudicação, dor a flexão passiva, diminuição da mobilidade articular e aumento de volume articular. No entanto, o tratamento produziu um efeito condroprotetor e anabólico sobre tecido de reparo formado, uma vez que o grupo tratado apresentou menor concentração de ácido hialurônico 3, 45 e 90 dias e melhor escore macroscópico ICRS aos 210 dias / Cartilage defects and the most common joint disease, osteoarthritis, are characterized by destruction of articular cartilage, and consequently in loss of joint function in humans and animals. Current strategies of conservative and surgical treatment are insufficient: they don&rsquo;t result in complete restoration of hyaline cartilage, and bring a poor prognosis on the long term. This study aims to evaluate the effects of co-administration of bone marrow concentrate, platelet rich plasma on experimentally induced chondral lesions and treated with microfractures and hyaluronic acid. The metacarpophalangeal joints of 6 mares were used, which were divided into 2 groups at random and blind. Total chondral defects were made using arthroscopy, and all were treated with microfractures and hyaluronic acid during surgery (M 0) and the hyaluronic acid was repeated after 15 days (M 15) and 30 days (M 30). Group C (control) and T group (treated). Group T was treated with concentrated aspirated bone marrow stem cells added to the platelet rich plasma (PRP), which were injected into the joint at surgery (M0). The T group had another 2 additional joint PRP applications in 15 days (M 15) and 30 days (M 30). The following evaluations were performed: clinical examination of lameness, ultrasound, radiographic studies and synovial fluid analysis (physical, biochemical and cytological). The evaluations were performed before surgery (M 0), 3, 5 and 7 days. Then, every 15 days (M 15, M 30, M 45 and M 60) and the last 3 evaluation were at 90 (M90), 120 (M120) and 210 (M210) days. At the end of the experiment the animals were sent to commercial slaughter. Statistical differences were found (p <0.05) between the treated and control group for evaluation of protein in synovial fluid corrected by urea at 3, 5 and 7 days; for PGE2 in the synovial fluid in 3 to 5 days where both variables had higher values for the treated group. The hyaluronic acid concentration was higher (p <0.05) at 3, 45 and 90 days in the control group. For the other variables there were no statistical difference between the treated and control groups. However, greater medians were noticed for chondroitin sulfate in 3 to 5 days in the control group. Notably, the treated group showed better macroscopic score in the evaluation of the repair tissue. In conclusion, intra-articular administration of bone marrow concentrate and platelet-rich plasma on induced chondral lesions and treated with microfractures and hyaluronic acid produced a transient response joint, especially during the first 60 days, and it was evidenced by the increase in PGE2 and proteins of the synovial fluid, as well as lameness, pain passive flexion, decreased joint mobility and joint swelling. Besides that, the treatment produced an anabolic chondroprotective effect on repair tissue formed once the treated group showed lower concentration of hyaluronic acid 3, 45 and 90 days, and better ICRS macroscopic scoring at 210 days

L'analyse de défaillances joue un rôle important dans l'amélioration des performances et de la fabrication des circuits intégrés. Des défaillances peuvent intervenir à tout moment dans la chaîne d'un produit, que ce soit au niveau conception, durant la qualification du produit, lors de la production, ou encore lors de son utilisation. Il est donc important d'étudier ces défauts dans le but d'améliorer la fiabilité des produits. De plus, avec l'augmentation de la densité et de la complexité des puces, il est de plus en plus difficile de localiser les défauts, et ce malgré l'amélioration des techniques d'analyses. Ce travail de thèse s'inscrit dans ce contexte et vise à étudier et développer une nouvelle technique d'analyse de défaillance basée sur l'étude de l'onde laser réfléchie le "Laser Voltage Imaging" (LVI) pour l'analyse de défaillance des technologies ultimes (inférieur à 45nm). / The Failure analysis plays an important role in the improvement of the performances and themanufacturing of integrated circuits. Defects can be present at any time in the product chain,during the conception (design), during the qualification, during the production, or still duringits use. It is important to study these defects in order to improve the reliability of the products.Furthermore, with the density increasing and the complexity of the chips, it is harder andharder to localize the defects. This thesis work consists to develop a new failure analysis technique based on the study of thereflected laser beam the "Laser Voltage Imaging" LVI, for the ultimate technologies (below45nm).

L’objectif de cette étude est de mieux comprendre le rôle joué par les défauts dans les propriétés optiques et électroniques des nanostructures d’oxyde de zinc. Pour ce faire, nous avons synthétisé des nanoparticules d’oxyde de zinc de 6 à 18 nm de diamètres pouvant être considérées comme modèle en terme de stœchiométrie, de cristallinité et de qualité de surface par une méthode physique originale : la Low Energy Cluster Beam Deposition.La caractérisation optique des défauts présents dans les nanoparticules de ZnO a été faite grâce à l’analyse des spectres d’émission visible et UV à différentes températures [10K-300K]. En particulier la luminescence excitonique à 3,31 eV, qui est un sujet controversé, a été étudiée en comparant la luminescence excitonique d’échantillons structurés à différentes échelles (nanoparticules, microcristaux et monocristal). Les temps de déclins très rapides des défauts donneurs ont été étudiés par spectroscopie à décalage de fréquence au CELIA à Bordeaux révélant une dépendance en fonction de la taille des NPs du type Giant Oscillator Strenght.Les propriétés de transport électronique des couches minces de NPs, naturellement dopées n, ont été caractérisées grâce à des expériences σ(T). Différents scénarios sont proposés pour expliquer les résultats des expériences de conductivité, et discutés en fonction des propriétés optiques des couches et de leur morphologie. En particulier, il est montré que la surface des NPs, très réactive, influence fortement le transport, ce qui laisse entrevoir la possibilité d’utiliser ces films nanostructurés comme capteurs de gaz. / This study deals with the influence of defects on the electronic properties of ZnO nanoparticles (NPs).In order to perform this study we have synthesized ZnO NPs using an original physical way : the Low Energy Cluster Beam Deposition. The NPs size can be adjusted between 6 and 18 nm depending the synthesis parameters and their analysis shows that the NPs have a good stoichiometry, cristallinity and surface quality.The photoluminescence properties of different ZnO samples structured at different scales (i.e. nanoparticle, microcrystal and monocrystal) have been analyzed at different temperature (10K-300K). The good cristallinity of the NPs is confirmed by the lack of visible luminescence. The comparison of the 3,31 eV excitonic emission of ZnO samples structured at different scales shows that this band is due to extended defect and/or exciton-phonon coupling rather than a surface luminescence. Moreover the lack of the 3.31 eV in the NPs luminescence shows that these defect does not occurs in ZnO NPs. At last, the time resolved study of the donor bounded exciton emission shows a variation of the decay time with the size of NPs suggesting a Giant Oscillator Strenght phenomenon.The transport properties of ZnO NPs assembled thin film are determined by conductivity measurements at variable temperatures. Different possibilities are considered in order to explain the temperature dependence of the conductivity and correlated with the optical properties of the NPs, showing a hopping type conductivity. The huge reactivity of the nanostructured film strongly influences the conductivity showing a possibility to use ZnO NPs as a gas sensor.

Dans ce travail, de nouveaux développements sur les techniques de localisation des composants électroniques en trois dimensions sont montrés. Ces développements sont réalisés grâce à l'introduction de simulations pour une technique déjà existante: la Microscopie Magnétique (MM). Dans la première partie, l'état de l'art de l'assemblage des nouveaux composants tridimensionnels est décrit. Il est ensuite suivi par une description du processus FA actuel, tout en le gardant aussi général que possible. Une description de la fiabilité des dispositifs, en fonction de leur temps d'utilisation est décrite, permettant au lecteur de comprendre pourquoi initialement l'Analyse de défaillance est apparue nécessaire. L'ensemble du processus d'analyse de défaillance est alors décrit de manière générale, à partir de la caractérisation électrique du défaut, jusqu'aux résultats finaux. Dans la deuxième partie est ensuite expliquée dans le détail la technique de microscopie magnétique, qui utilise les propriétés des champs magnétiques générés par les courants, et permet de localiser précisément les défauts des composants électroniques standards. La troisième partie de ce travail est consacrée à l'approche de simulation (SA): une nouvelle méthodologie développée pour étendre les capacités des techniques de microscopie magnétique. Le principe de base est de comparer la simulation magnétique générée par des hypothèses de distributions de courant aux acquisitions magnétiques de la distribution réelle. L'évaluation de la corrélation entre les deux donnera ensuite une mesure de la distance entre eux. Par ailleurs, cette approche est capable de surmonter les limitations de la technique: le défaut peut désormais être localisé en trois dimensions. Enfin, dans la quatrième partie, la nouvelle technique est appliquée et validé sur un ensemble de cas d'études. / In this work, new developments on localization techniques for three-dimensional electronic components are shown and demonstrated. These are performed through the introduction of simulations for an already existing technique: Magnetic Microscopy (MM). In the first part, a state of the art of new three-dimensional components assembly is described. It is then followed by an up to date FA process description, while keeping it as general as possible. A description of component reliability, in function of the time of usage of such devices is shown, allowing the reader to understand why the need for Failure Analysis arose in the first place. The whole process of Failure Analysis is then described in a general way, starting from the electrical characterization of the defect, to the final results. The second part then explains the Magnetic Microscopy technique in more detail. This technique uses the properties of the magnetic fields, which are generated by the currents, to precisely localize the defects in standard electronic components. The third part of this work is dedicated to the Simulation Approach (SA): a new methodology developed to extend the capabilities of Magnetic Microscopy techniques. The basic principle is that of comparing magnetic simulations generated by hypothetical current distributions to the magnetic acquisitions of the real current distribution. The evaluation of the correlation between the two then gives a measurement of the distance between them. This approach is able to overcome the previous limitations of the technique: the defect can now be localized in three dimensions. Finally, in the fourth part the new technique is applied and validated on a set of case studies.

Ces travaux de thèse sont consacrés à l’étude de l’influence du procédé de poinçonnage sur la résistance en fatigue à grand nombre de cycles de tôles minces ferromagnétiques. Étant donné leurs propriétés magnétiques améliorées, ces tôles en alliage fer-silicium sont de plus en plus utilisées pour la fabrication des moteurs électriques qui tournent à des vitesses élevées. En effet, les pertes magnétiques dans ces tôles sont réduites par l’augmentation de la taille de grain, la diminution de l’épaisseur en dessous du demi‒millimètre et l’ajustement du pourcentage de silicium. L’objectif de cette étude est d’élaborer une démarche de dimensionnement en fatigue HCF des pièces en tôles minces poinçonnées. La première étape de cette démarche est la caractérisation expérimentale du matériau étudié. Des essais de traction monotone quasi-statique et d’écrouissage cyclique en utilisant différentes conditions ont permis de déterminer les grandeurs caractéristiques de cet alliage et d’identifier un modèle de comportement mécanique cyclique utilisable pour les simulations par la méthode des éléments finis (EF). L’étude de la résistance en fatigue de cette tôle est réalisée en utilisant des géométries d’éprouvettes lisses et entaillées. Différents effets tel que le rapport de charge, la température (180°C) et le procédé sont ainsi étudiés. Étant donné son influence sur la tenue en fatigue, l’effet du procédé de poinçonnage est finement investigué. Les bords des éprouvettes de fatigue sont analysés en utilisant des techniques variées (observations microscopiques, profilométrie optique, micro‒dureté, diffraction des rayons X…). Ceci a permis d’identifier les mécanismes d’endommagement par fatigue des pièces en M330‒35A poinçonnées. Ensuite, différentes configurations d’éprouvettes sont utilisées pour quantifier l’influence des différents effets induits par le procédé tel que l’écrouissage, les contraintes résiduelles et les défauts géométriques sur la résistance en fatigue de l’alliage étudié. Les résultats des investigations sur les bords ont montré que l’amorçage des fissures est localisé sur un défaut de poinçonnage. Une stratégie qui permet d’identifier les défauts critiques est alors adoptée. Des simulations (EF) réalisées sur les défauts de poinçonnage ont permis de déterminer les champs mécaniques autour de ces défauts. Enfin, une stratégie de dimensionnement en fatigue qui s’appuie sur l’utilisation d’un critère de fatigue non‒local en post‒traitement des résultats des simulations par EF sur les défauts de poinçonnage est proposée. / New electrical steel grades, with improved magnetic properties, are used to build electric motors. For these steel grades, the iron losses are reduced by adjusting the chemical composition (mostly the Si content), decreasing the thickness below 0.5 mm and increasing the grain size. The punching is used to produce electric motor components because it generates important alterations of sheet edges, this work aims at elaborating a HCf fatigue design strategy for thin punched electrical steel sheets. First, the quasi-static and cyclic behavior of this electric steel was studied through monotonic and cyclic tests. The behavior model of this material, which will be used in FE simulation, is then identified. The study of the high cycle fatigue (HCF) resistance of this material is performed using smooth and notched specimen’s geometries. The effect of stress ratio, temperature (180°C) and the punching process are considered. Due to its influence on the fatigue resistance, the effect of the punching process is finely investigated. Different experimental techniques such as microscopic observations, 3D surface topography, micro‒hardness and X‒ray diffraction are combined to characterize the specimen’s edges. To dissociate the respective influences of strain hardening, residual stresses and geometrical defects induced by the punching process, and to quantify the contribution of each parameter to the HCF resistance, different specimen’s configurations were tested. A strategy allowing the identification of the critical defects, on which fatigue crack initiation occurs, was adopted. The stress distribution around defects is determined from finite element analyses (FEA) on real defect geometries. A non‒local high cycle fatigue criterion is finally used as post‒processing of FEA to consider the effect of defects and the associated stress-strain gradients in the HCF strength assessment.

La méthode d'identification développée dans cette thèse est inspirée des travaux de D. Rémond. On considérera les données d'entrée suivante : la réponse de la structure, qui sera mesurée de manière discrète, et qui dépendra des dimensions de la structure (temps, espace) le modèle de comportement, qui sera exprimé sous forme d'une équation différentielle ou d'une équation aux dérivées partielles, les conditions aux limites ainsi que la source d'excitation seront considérées comme non mesurées, ou inconnues. La procédure d'identification est composée de trois étapes : la projection sur une base polynomiale orthogonale (polynômes de Chebyshev) du signal mesuré, la différentiation du signal mesuré, l'estimation de paramètres, en transformant l'équation de comportement en une équation algébrique. La poutre de Bernoulli a permis d'établir un lien entre l'ordre de troncature de la base polynomiale et le nombre d'ondes contenu dans le signal projeté. Sur un signal bruité, nous avons pu établir une valeur de nombre d'onde et d'ordre de troncature minimum pour assurer une estimation précise du paramètre à identifier. Grâce à l'exemple de la poutre de Timoshenko, nous avons pu réadapter la procédure d'identification à l'estimation de plusieurs paramètres. Trois paramètres dont les valeurs ont des ordres radicalement différents ont été estimés. Cet exemple illustre également la stratégie de régularisation à adopter avec ce type de problèmes. L'estimation de l'amortissement sur une poutre a été réalisée avec succès, que ce soit à l'aide de sa réponse transitoire ou à l'aide du régime établi. Le cas bidimensionnel de la plaque a également été traité. Il a permis d'établir un lien similaire au cas de la poutre de Bernoulli entre le nombre d'onde et l'ordre de troncature. Deux cas d'applications expérimentales ont été traités au cours de cette thèse. Le premier se base sur le modèle de la poutre de Bernoulli, appliqué à la détection de défaut. En effet on applique un procédé d'identification ayant pour hypothèse initiale la continuité de la structure. Dans le cas où celle-ci ne le serait pas on s'attend à observer une valeur aberrante du paramètre reconstruit. Le procédé permet de localiser avec succès le lieu de la discontinuité. Le second cas applicatif vise à reconstruire l'amortissement d'une structure 2D : une plaque libre-libre. On compare les résultats obtenus à l'aide de notre procédé d'identification à ceux obtenus par Ablitzer à l'aide de la méthode RIFF. Les deux méthodes permettent d'obtenir des résultats sensiblement proches. / The purpose of this work is to adapt and improve the continuous time identification method proposed by D. Rémond for continuous structures. D. Rémond clearly separated this identification method into three steps: signal expansion, signal differentiation and parameter estimation. In this study, both expansion and differentiation steps are drastically improved. An original differentiation method is developed and adapted to partial differentiation. The existing identification process is firstly adapted to continuous structure. Then the expansion and differentiation principle are presented. For this identification purpose a novel differentiation model was proposed. The aim of this novel operator was to limit the sensitivity of the method to the tuning parameter (truncation number). The precision enhancement using this novel operator was highlighted through different examples. An interesting property of Chebyshev polynomials was also brought to the fore : the use of an exact discrete expansion with the polynomials Gauss points. The Gauss points permit an accurate identification using a restricted number of sensors, limiting de facto the signal acquisition duration. In order to reduce the noise sensitivity of the method, a regularization step was added. This regularization step, named the instrumental variable, was inspired from the automation domain. The instrumental variable works as a filter. The identified parameter is recursively filtered through the structure model. The final result is the optimal parameter estimation for a given model. Different numerical applications are depicted. A focus is made on different practical particularities, such as the use of the steady-state response, the identification of multiple parameters, etc. The first experimental application is a crack detection on a beam. The second experimental application is the identification of damping on a plate.

This diploma thesis deals with the evaluation of the constructions of the industrial buildings according to the ČSN ISO 13822 Standard. In the first, theoretical part, the procedures of research and evaluation of existing reinforced concrete constructions based on the ČSN ISO 13822 and ČSN 73 0038 Standards are described. Further, the theoretical part is focused on the diagnostic methods used during the evaluation of these constructions. The practical part focuses on the evaluation of the three industrial reinforced concrete buildings. It includes information about the building, the description of defects, diagnostics and the appraisal of measuring. The last part deals with a static calculation of selected parts of the supporting construction.

Abstract This thesis presents measuring techniques as well as measured and simulated results with the aim of helping the design of photovoltaic energy harvesting systems. Therefore, cost-effective measurement setups were developed for collecting the amount of irradiation, for both stationary and moving photovoltaic (PV) installations. The impact of the time resolution of solar radiation data on estimating the available solar energy was investigated. For moving PV installations, the dynamics and the rate of changes in the available irradiation were studied in order to analyse the effects on maximum power point tracking (MPPT) algorithms. In addition, possibilities for harvesting PV energy in indoor environments were also investigated. The main contribution of this thesis is the effective testing of PV cells and complete PV panels: instead of measuring the characteristic I-V (Current-Voltage) response under strictly controlled artificial illumination, photovoltaics are simply biased externally. Then, with the help of synchronized thermography (ST), infrared (IR) images of the PV panel self-heating are recorded. In the obtained IR-images, defected areas are seen as cold spots, since they are not biased by the external power supply. From the calculated temperature variations, the size of the defect area can be calculated and, thus, the loss in output power can be estimated. The method is shown to work both with and without glass encapsulation. / Tiivistelmä Tämä työ esittelee mittaustekniikoita ja mitattuja ja simuloituja tuloksia aurinkoenergian keruujärjestelmien suunnittelun avuksi. Työtä varten kehitettiin kustannustehokas mittausjärjestelmä, jonka avulla arvioitiin aurinkoenergian määrää sekä stationaarisen että liikkuvan valokennon tapauksissa. Näiden lisäksi tutkittiin mittaustaajuuden vaikutusta arvioitaessa saatavilla olevan aurinkoenergian määrää. Liikkuvan PV (photovoltaic)-asennuksen avulla tutkittiin saatavilla olevan aurinkoenergian vaihtelun suuruutta ja nopeutta tarkoituksena analysoida näiden vaikutuksia käytettäviin MPPT-algoritmeihin. Tämä lisäksi tutkittiin myös valoenergian keruumahdollisuuksia sisätiloissa. Työn tärkein kontribuutio on valokennojen ja kokonaisten valopaneelien toiminnallisuuden testaamisen tehostaminen. Tyypillisesti PV:n toiminnallisuus varmistetaan tarkasti määritetyssä ympäristössä suoritetun I-V -ominaiskäyrämittauksen avulla. Tämän työn menetelmä on yksinkertaisesti biasoida PV:t ulkoisesti, minkä jälkeen ST (synchronized thermpgraphy) -kuvauksen avulla määritetään PV-paneelien itselämpenemistä kuvaavat infrapunakuvat. Paneelin vioittuneet alueet erottuvat IR-kuvissa kylminä alueina ulkoisen biasoinnin puuttuessa. IR-kuvista havaituista lämpötilavaihteluista on mahdollista määrittää vioittuneen alueen koko ja siten arvioida myös menetettyä lähtötehoa. Kyseisen metodin toimivuus osoitettiin niin lasikoteloiduilla kuin ilman sitä olevilla PV-paneeleilla.

Mesoscopic structures in liquids have an impact on the diffusion dynamics of the constituting molecules. Smectic 8CB liquid crystals on silicon wafers show the formation of mesoscopic structures on the μm scale at a film thickness of 200 nm. Depending on the kind of substrate (thermally grown or native SiOx), we observed the formation of focal conic domains (FCDs) and a new type of terraced holes, respectively. Dynamics are described via single perylene diimide tracer molecule tracking of translational diffusion and in the case of FCDs by a combination of translation and rotation detected via fluorescence correlation spectroscopy. Tailoring perylene diimide molecules such that the optical transition dipole moment follows the liquid crystal director allows mapping out FCDs and investigating the dynamics within a single FCD.