Submitted by Alison Vanceto (alison-vanceto@hotmail.com) on 2016-10-04T12:38:10Z No. of bitstreams: 1 DissYLSM.pdf: 5457505 bytes, checksum: 5b644703e5553e5b7a06e6ad6e976868 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2016-10-04T18:00:12Z (GMT) No. of bitstreams: 1 DissYLSM.pdf: 5457505 bytes, checksum: 5b644703e5553e5b7a06e6ad6e976868 (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2016-10-04T18:00:21Z (GMT) No. of bitstreams: 1 DissYLSM.pdf: 5457505 bytes, checksum: 5b644703e5553e5b7a06e6ad6e976868 (MD5) / Made available in DSpace on 2016-10-04T18:13:36Z (GMT). No. of bitstreams: 1 DissYLSM.pdf: 5457505 bytes, checksum: 5b644703e5553e5b7a06e6ad6e976868 (MD5) Previous issue date: 2012-11-01 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / The interaction between humans and the computers has improved substantially during time through the evolution of interfaces of interaction. The possibility of users to interact with machines through several modalities of communication, and in a natural way, can increase the level of interest from the user and ensure the success of the application. However, the literature of the area of multimodality has shown that developing such interfaces is not a simple task, mainly for non-experienced or recently graduated professionals, since each designer’s modality of interaction has its complexity in technical terms, as acquisition and adaptation with new tools, languages, possible actions and etc. Moreover it is necessary to verify which modalities (voice, touch and gestures) can be used in the application, how to combine these modalities in a way that the stronger point of one completes the weak point of the other and vice versa, and also knowing in what context the final user will be involved. The GuiBuilder Multimodal was developed aiming to try providing the basic needs in implementing an interface that uses voice, touch and gesture. The framework promotes an interface development through the WYSIWYG (What You See Is What You Get) model, where the designer just sets some parameters so the component is multimodal. During the interface creation phase, agents supervise what the designer does and supply support, clues, with design patterns that might be divided in categories such as: multimodality, interaction with the user and components. / A interação entre humano e computador tem melhorado substancialmente ao longo do tempo através da evolução das interfaces de interação. A possibilidade de usuários interagirem com máquinas através de várias modalidades de comunicação, e de forma natural, pode aumentar o nível de interesse do usuário e garantir o sucesso da aplicação. Porém, o estado da arte da área de multimodalidade demonstra que desenvolver tais interfaces não é uma tarefa simples, principalmente para projetistas inexperientes ou recém formados, pois cada modalidade de interação tem sua complexidade em termos técnicos, como aquisição e adaptação com novas ferramentas, linguagens, ações possíveis e etc. Além disso, é preciso verificar quais modalidades (voz, toque e gestos) podem ser usadas na aplicação, como combinar essas modalidades de forma que o ponto forte de uma complemente o ponto fraco da outra e vice-versa e também saber em qual contexto o usuário final estará inserido. O GuiBuilder Multimodal foi desenvolvido com o intuito de tentar suprir as necessidades básicas em se implementar uma interface que utiliza voz, toque e gesto. O framework promove um desenvolvimento de interface através do modelo WYSIWYG (What You See Is What You Get) onde o projetista apenas define alguns parâmetros para que um componente seja multimodal. Durante a fase de criação da interface agentes supervisionam o que o designer faz e fornece um apoio, dicas, com padrões de projeto que podem ser divididos em categorias como: multimodalidade, interação com o usuário e componentes.

Made available in DSpace on 2016-03-15T19:37:51Z (GMT). No. of bitstreams: 1 Andre Brasiliano da Silva.pdf: 4815626 bytes, checksum: e53fa837ff6a7eb3cff0f55f4b3b26ac (MD5) Previous issue date: 2014-08-21 / Eye tracking is an important issue for Human Computer interaction, mainly for users with hand-eye coordination problems. The work presented here shows a low cost and robust eye tracking system capable to work with an HD stream. The implementations used in this work over the base system present diferent techniques in all stages, from face detection to iris detection. Local processing is used in most stages in this implementation, delimiting the region of interest (ROI) for face detection, eye detection and iris detection. The system robustness allow the eye tracking system to control the mouse using eye movements allowing disable users to communicate through a communication interface. The hardware required is simple and based in an high definition webcam. The face detection and eye detection processes are based on the Viola Jones technique; iris detection and tracking are based on the Hough Transform. The usage of local processing reduces the computational cost and even working with high definition stream leads to a performance 33% better than the base system. The system presented here was compared with a commercial system and a set of equipment were tested in order to dene the best set up for the eye tracking system and to validate the work presented here. Future work is presented at the end in order to allow the project continuity. / O rastreamento ocular para usuários com problemas motores é um estudo importante na área de Interface Humano-Computador (IHC). Com o objetivo de fornecer um sistema de rastreamento ocular de baixo custo, este trabalho apresenta uma nova abordagem para um sistema robusto e com alto desempenho. Com relação ao trabalho base para esta pesquisa, a implementação proposta contém inovações em todas as etapas do processo envolvendo o rastreamento ocular, desde a detecção da região da face e dos olhos até a detecção da íris. Neste trabalho, foi utilizado o conceito de processamento local, delimitando as regiões de interesse em todas as etapas do processo: detecção da região da face, região dos olhos e região da íris. Este trabalho permite que pessoas possam efetuar ações controlando o mouse através do movimento dos olhos em uma interface de rastreamento ocular, utilizando apenas equipamentos de uso comum, como, por exemplo, uma webcam. O processo de detecção da face e detecção ocular foi feito através da técnica de Viola e Jones. Para a detecção e rastreamento da íris foi utilizada a Transformada de Hough, e utilização de regiões de interesse com o objetivo de limitar a área de processamento da imagem, e consequentemente, o custo computacional, resultando em uma aplicação com um melhor desempenho e robustez em todas as etapas. Obteve-se um ganho de até 33% em relação ao tempo de processamento do sistema, quando comparado com o sistema base, porém, operando com imagens em alta definição. Foi realizada ainda uma comparação com sistemas de rastreamento ocular de uso comercial e diferentes tipos de equipamentos para validar as técnicas estudadas neste trabalho.

No description available.

[pt] A busca na Web se tornou o método predominante para as pessoas suprirem suas necessidades de informação. Embora seja difundido, o modelo tradicional de páginas de resultados de pesquisa só é satisfatório se o usuário souber, com bastante precisão, como elaborar sua consulta para corresponder à busca das informações desejada. Propomos um novo modelo para páginas de resultados de pesquisa, que vai além de fornecer uma lista navegável de resultados em forma de visualizações, através da geração implícita de consultas relacionadas para expandir o espaço de busca, revelando progressivamente os resultados correspondentes. / [en] Web search has become the predominant method for people to fulfill their information needs. Although widespread, the traditional model for search result pages is only satisfactory if the user knows quite precisely how to phrase their query to match their intended information. We propose a new model for search page results, which goes beyond providing a navigable list of visualization search results, by implicitly generating related queries to expand the search space and progressively disclosing the corresponding results.

Syfte: Är att ta reda på om man med hjälp av immunhistokemi (IHC) med infärgning avantikroppar mot laminin-5 och C3d kan särskilja mellan slemhinnepemfigoid (MMP) ochorala lichenoida reaktioner (OLR) med epitelsläpp. Vidare undersöks graden inflammation för MMP och OLR för att fastställa om det går att se ett samband mellan grad av inflammation och antikroppsinfärgning.Material och metod: En pilotstudie utfördes på 10 prover med diagnosen MMP respektive 9 prover med OLR, som hämtades från Malmö universitets biobank. Proverna genomgickrutinfärgning respektive antikroppsinfärgning mot laminin-5 och C3d. Granskning av prover skedde i digitalmikroskop. Efteråt delades proverna in i grupper efter var infärgningen sågs. Sammanställning gjordes i Excel med stapeldiagram.Resultat: Ingen tendens till särskiljning ses mellan MMP och OLR avseende infärgning mot laminin-5 och C3d. Positiva utslag för infärgning mot Laminin-5 ses enhetligt på enbart en sida om epitelsläppet hos både snitten för MMP och OLR. Vidare kan det inte ses att snitt med diagnosen OLR har slumpartad infärgning mot laminin-5 på båda sidor om släppet. Det finns en tendens till att MMP-snitt får mer positiva utslag för infärgning mot C3d än för OLR snitt. Graden inflammation var högre i OLR snitt än för MMP snitt.Slutsats: Enligt studien går det inte att, med IHC, med infärgning av antikroppar mot laminin5 och C3d, kunna särskilja MMP och OLR med epitelsläpp. Större urval krävs för definitiva slutsatser. Vidare studier behövs för att utreda om det går att använda IHC för att särskilja MMP och OLR.Nyckelord: C3d, immunhistokemi (IHC), laminin-5, orala lichenoida reaktioner (OLR),slemhinnepemfigoid (MMP) / Aim: To investigate if it is possible to differentiate between the diagnoses mucous membrane pemphigoid (MMP) and oral lichenoid reactions (OLR) with epithelial detachment using immunohistochemistry (IHC) with antibodies against laminin-5 and C3d. Furthermore, the extent of inflammation was examined for MMP and OLR to determine if a correlation between the inflammation and immunostaining is evident.Material and method: A pilot study is conducted using 10 samples diagnosed with MMP and 9 samples diagnosed with OLR, collected from Malmö University’s biobank. H&E staining and immunostaining against laminin-5 and C3d is performed on the samples. Analysis is conducted using a microscope. Samples are then divided into groups depending on the staining. Excel is used to compile the results.Result: No differentiating tendencies are observed between MMP and OLR regardingimmunostaining against laminin-5 and C3d. Immunostaining against laminin-5 is positive for MMP and OLR and is seen continuously on only one side of the epithelial detachment.Furthermore, staining with laminin-5 is not seen as random staining on both sides of theepithelial detachment. Samples with MMP have a higher tendency to stain against C3dcompared to OLR samples. Inflammation is higher in OLR samples than those for MMP.Conclusion: According to this study it is not possible to differentiate between the diagnoses MMP and OLR with epithelial detachment using immunostaining against laminin-5 and C3d. A larger sample size is needed for a definitive conclusion. Additionally, further studies are required to conclude if IHC can be used to differentiate between MMP and OLR.Keywords: C3d, immunohistochemistry (IHC), laminin-5, mucous membrane pemphigoid(MMP), oral lichenoid reactions (OLR)

Streptococcus agalactiae is a pathogen of multiple hosts. The bacterium, an aetiological agent of septicaemia and meningo-encephalitis in freshwater and saltwater fish species, is considered a major threat to the aquaculture industry, particularly for tilapia. Cattle and humans are however the main known reservoirs for S. agalactiae. In humans, the bacterium (commonly referred to as Group B Streptococcus or GBS) is a member of the commensal microflora of the intestinal and genito-urinary tracts, but it is also a major cause of neonatal invasive disease and an emerging pathogen in adults. In cattle, S. agalactiae is a well-recognized causative agent of mastitis. Numerous studies focusing on S. agalactiae from human and bovine origins have provided insight into the population structure of the bacterium, as well as the genome content and pathogenic mechanisms through identification of virulence determinants. Concerning S. agalactiae from aquatic origins, scientific information mainly focused on case reporting and/or experimental challenges, with a limited or absence of information in terms of pathogenesis, virulence determinants and genotypes of the strains involved. The objective of this study was to enhance our understanding of the molecular epidemiology, host-adaptation and pathogenicity of S. agalactiae in aquatic species, with particular emphasis on tilapia. Firstly, a collection of 33 piscine, amphibian and sea mammal isolates originating from several countries and continents was assembled, with the aim of exploring the population structure and potential host specificity of aquatic S. agalactiae. Isolates were characterised using pulsed-field gel electrophoresis (PFGE), multi-locus sequence typing (MLST), and a standardised 3-set genotyping system comprising molecular serotypes, surface protein gene profiles and mobile genetic element profiles. Two major subpopulations were identified in fish. The first subpopulation consisted of non-haemolytic isolates that belonged to sequence type (ST) 260 or 261, which are STs that have been reported only from teleosts. These isolates exhibited a low level of genetic diversity by PFGE and clustered with other STs that have been reported only in fish. Another common feature was the absence of all surface protein genes or mobile genetic elements targeted as part of the 3-set genotyping and that are usually found in human or bovine isolates. The second subpopulation consisted of β-haemolytic isolates recovered from fish, frogs and sea mammals, and that exhibited medium to high genetic diversity by PFGE. STs identified among these isolates have previously been identified from strains associated with asymptomatic carriage and invasive disease in humans. The human pathogenic strain ST7 serotype Ia was detected in fish from Asia. Moreover, ST283 serotype III-4 and its novel single locus variant ST491 detected in fish from Southeast Asia shared a 3-set genotype identical to that of an emerging ST283 clone associated with invasive disease of adult humans in Asia. These observations suggested that some strains of aquatic S. agalactiae may present a zoonotic or anthroponotic hazard. STs found among the seal isolates (ST23) have also been reported from humans and numerous other host species, but never from teleosts. This work provided an excellent basis for exploration of the virulence of selected strains in experimental challenges. The virulence of two strains of S. agalactiae was experimentally investigated by intra-peritoneal infection of Nile tilapia (Oreochromis niloticus), using an isolate originally recovered from fish and belonging to ST260, and an isolate originating from a grey seal and belonging to ST23. The clinical signs, the in vivo distribution of viable bacteria and bacterial antigens, and the gross and histopathological lesions that developed during the time course of the infection were investigated. The ST260 strain was highly virulent, whereas no major clinical sign or mortalities occurred in the fish challenged with the ST23 strain. After injection, both strains however gained access to the bloodstream and viable bacteria were recovered from all organs under investigation. During the early stages of infection, bacteria were mostly found within the reticulo-endothelial system of the spleen and kidney. Thereafter, the ST260 demonstrated a particular tropism for the brain and the heart, but granulomatous inflammation and associated necrotic lesions were observed in all organs. ST23 was responsible for a mixed inflammatory response associated with the presence of bacteria in the choroid rete and in the pancreatic tissue only. After 7 days post-challenge and for both strain, the formation or containment of bacteria within granulomata or other encapsulated structures appeared to be a major component of the fish response. However, the load of viable bacteria remained high within organs of fish infected with ST260, suggesting that, unlike ST23, this strain is able to survive within macrophages and/or to evade the immune system of the fish. This work demonstrates that the lack of report of ST23 strains in fish is possibly not due to a lack of exposure but to a lack of virulence in this host. The two strains, which differ in prevalence and virulence in fish, provide an excellent basis to investigate genomic differences underlying the host-association of distinct S. agalactiae subpopulations. The genome of the ST260 strain used in challenge studies was sequenced. We therefore provided the first description for the genome sequence of a non-haemolytic S. agalactiae isolated from tilapia (strain STIR-CD-17) and that belongs by multi-locus sequence typing (MLST) to clonal complex (CC) 552, which corresponds to a presumptive fish-adapted subgroup of S. agalactiae. The genome was compared to 13 S. agalactiae genomes of human (n=7), bovine (n=2), fish (n=3) and unknown (n=1) origins. Phylogenetic analysis based on the core genome identified isolates of CC552 as the most diverged of all S. agalactiae studied. Conversely, genomes from β-haemolytic isolates of CC7 recovered from fish were found to cluster with human isolates of CC7, further supporting the possibility that some strains may represent a zoonotic or anthroponotic hazard. Comparative analysis of the accessory genome enabled the identification of a cluster of genes uniquely shared between CC7 and CC552, which encode proteins that may provide enhanced fitness in specific niches. Other genes identified were specific to STIR-CD-17 or to CC552 based on genomic comparisons; however the extension of this analysis through the PCR screening of a larger population of S. agalactiae suggested that some of these genes may occasionally be present in isolates belonging to CC7. Some of these genes, occurring in clusters, exhibited typical signatures of mobile genetic elements, suggesting their acquisition through horizontal gene transfer. It is not possible to date to determine whether these genes were acquired through intraspecies transfer or through interspecies transfer from the aquatic environment. Finally, general features of STIR-CD-17 highlighted a distinctive genome characterised by an absence of well conserved insertion sequences, an abundance of pseudogenes, a smaller genomic size than normally observed among human or bovine S. agalactiae, and an apparent loss of metabolic functions considered conserved within the bacterial species, indicating that the fish-adapted subgroup of isolates (CC552) has undergone niche restriction. Finally, genes encoding recognised virulence factors in human S. agalactiae were selected and their presence and structural conservation was evaluated within the genome of STIR-CD-17.

Genes da família homeobox têm sido alvo de intensas pesquisas científicas relacionadas ao câncer. Recentemente, mostramos que o gene homeobox TGIF1 está expresso no carcinoma epidermóide de boca na sua forma genérica. Porém, não foi feita uma discriminação entre quais variantes transcricionais, ou subtipos, do TGIF1 estariam expressas. Neste estudo, propusemo-nos a verificar diferenças na freqüência de expressão das variantes transcricionais do TGIF1 em carcinomas epidermóides de boca (CEB) em relação a tecidos morfologicamente não tumorais (TN), avaliar possíveis associações entre essas variantes nos pacientes portadores de CEB e relacionar o grau de expressão dessas variantes com aspectos clínicos, histológicos e com a sobrevida dos pacientes. Adicionalmente, procuramos analisar a expressão da proteína do TGIF1. Foram analisadas 48 amostras congeladas de CEB e 12 de TN. O RNA total de cada amostra foi extraído utilizando-se solução de TRizol®. Os transcritos do TGIF1 foram amplificados por RT-PCR para cada caso de CEB e TN utilizando-se inicialmente um par de iniciadores genéricos para todas as suas variantes. Após essa triagem, os casos positivos foram amplificados utilizando-se pares de iniciadores específicos para cada variante. Não houve diferença estatística entre a freqüência de expressão das variantes do TGIF1 nos grupos CEB e TN, porém as variantes 4 e 8 foram as que apresentaram p-valores menores. Dentro do grupo de CEB, houve associação significativa entre algumas variantes entre si (sete dos vinte e um cruzamentos), de forma que as variantes 1 e 4 foram as que mais tiverem associação com outras variantes. Dessa forma, optamos por prosseguir o estudo utilizando somente as variantes 1, 4 e 8. Não houve correlação entre a expressão das variantes selecionadas e variáveis clinicas e histológicas propostas. Em relação à sobrevida, a expressão das variantes 1 e 8 mostraram correlação univariada com o desfecho óbito, de maneira que o grupo de indivíduos que mais expressou ambas as variantes foi o que apresentou menor risco de óbito. Através da análise multivariada das variantes 1 e 8 e aspectos clínicos e histológicos, somente o tamanho da lesão e a invasão vascular sanguínea tiveram significado estatístico. A expressão protéica do TGIF1 foi analisada em 46 amostras parafinadas considerando-se a diferenciação celular, a graduação imunoistoquímica e o compartimento celular. Os resultados obtidos mostraram que as variantes do TGIF1 estão expressas diferentemente no grupo dos pacientes com CEB e sugerese que a expressão das variantes 1 e 8 estejam relacionadas, de maneira semelhante, a um menor risco de óbito para pacientes portadores de CEB. Adicionalmente, sugere-se que o tamanho da lesão e a invasão vascular sanguinea representem fatores de risco relevantes para o óbito de pacientes portadores de CEB. Sugere-se também que a expressão simultânea da proteína do TGIF1 tanto no núcleo quanto no citoplasma da célula esteja correlacionada a lesões pobremente diferenciadas. / Genes of the homeobox family have been the subject of intense scientific research related to cancer. Recently, we show that this gene is expressed in oral squamous cell carcinoma in its generic form. However, it was not made a discrimination between which transcript variants, or subtypes\", of TGIF1 were expressed. In this study, we aim to verify differences in the expression of the eight transcript variants described for the homeobox gene TGIF1 in oral squamous cell carcinomas (OSCC) related with morphologically non-tumoral tissues (NT), evaluate possible associations between these variants in patients with OSCC and relate the degree of expression of these variants with clinical, histological and survival aspects of patients. Additionally, we analyzed the expression of TGIF1 protein. It was analyzed 48 frozen samples of OSCC and 12 of NT. The total RNA from each sample was extracted using TRizol solution. TGIF1 transcripts were first amplified by RT-PCR for each case of OSCC and NT using a generic pair of primers. After these screening, the positive cases were amplified using specific pair of primers for each variant. There was no statistical difference between the frequency of expression of TGIF1 transcript variants in OSSC and NT groups, but the variants 4 and 8 had the lowest p-values. Within the OSCC group, there was a significant association between some variants among themselves (seven of the twenty-one associations), in a way that the variants 1 and 4 were the ones that had most association with other variants. Thus, we chose to continue the study using only variants 1, 4 and 8. There was no correlation between the expression of the selected variants with the clinical and histological aspects. Regarding survival, the expression of variants 1 and 8 showed statistical correlation with the outcome death, in a way that the group of patients that most expressed both variants was that one with the lower risk of death. By multivariate analysis of variants 1 and 8 and clinical and histological aspects, only the size of the lesion and vascular invasion blood were statistically significant. The protein expression of TGIF1 was analyzed in 46 paraffin-embedded tissues considering the cell differentiation, the immunohistochemistry graduation and the cell compartment. The results showed that the variants of TGIF1 are differently expressed in the OSCC group of patients and it is suggested that the expression of variants 1 and 8 may be related, in a similar way, to a lower risk of death for patients with OSCC. Additionally, it is suggested that the size of the lesion and the vascular invasion of blood represent relevant risk factors for death in patients with OSCC. It is also suggested that the simultaneous expression of TGIF1 protein not only in the nucleus but also in the cytoplasm of the cell is correlated with the poorly differentiated lesions of OSCC.

Made available in DSpace on 2016-04-26T18:18:31Z (GMT). No. of bitstreams: 1 Paulo Alves de Lima.pdf: 91765055 bytes, checksum: 8c46ce0bc23b5f4635e078ef4a6fe5da (MD5) Previous issue date: 2010-03-08 / Considering the current dissemination stage of the world wide web and the historical social repercussion which such presence evokes, this Master Thesis intents to provide subsidies to webdesign activity, developing knowledges that contribute to the process of infographics elements manipulation in the web interfaces construction. The study corpus, Brazilian websites graphical interfaces, required for its analysis, quali-quantitative empirical research that enables, through aesthetic dissection, the identification of the existence of dominating visual patterns in Brazilian websites interfaces. The results of this stage reference the elaboration of representative models, which in the following step passed by a test process and validation with the users. The successful achievement of these objectives made it possible to solve one of the major problems related to the research object, the assembly of an interface model capable of tracing and computing path of user access, identifying separately how the infographics elements of the screen are related to each other, in the reception context, to the surfer decision for the click-through. This road bared the existence evidences of aesthetic phenomenal in the research context, fact theoretically addressed through style viewpoint, which resulted in proposing a new approach to the webdesign, capable of bringing closer the cyberculture theoretical views and the empirical results produced nowadays. The theoretical framework of the dissertation is based on three great quadrants. In the first one is located the comprehension, in the applied social science context, of the historical social movie set where the cyberculture and its typical technologically mediated communication processes are inserted. On this stage, the references are Zygmunt Bauman, Paul Virilio, Neil Postman, Asa Briggs and Peter Burke. In a second moment, the computer graphic interfaces genesis is treated with their semantic machines and graphical metaphors; here the text dialog extends to propositions of Steven Johnson, Oliver Sacks, Philippe Breton and Walter Benjamin. Finally, follows a reflection about the webdesign and its conceptualaesthetic issues under the historical view of style and here the references are Adrian Frutiger, Josef Albers, Allen Hurkburt, Donis Dondis, Charles Peirce, Eugênio Trivinho e Lucia Santaella / Considerando o atual estágio de disseminação da grande rede mundial de computadores e a repercussão social-histórica que tal presença evoca, esta Dissertação de Mestrado pretende oferecer subsídios à atividade de webdesign, desenvolvendo conhecimentos que contribuam para o processo de manipulação dos elementos infográficos na construção de interfaces Web. O corpus do estudo, as interfaces gráficas dos websites brasileiros, exigiu, para a sua análise, pesquisa empírica qualiquantitativa que possibilitasse, através de dissecação estética, identificar a existência de padrões visuais dominantes nas interfaces de websites brasileiros. Os resultados dessa etapa referenciaram a elaboração de modelos representativos, que em etapa seguinte passaram por processo de teste e validação junto a usuários. O cumprimento exitoso desses objetivos tornou possível solucionar um dos principais problemas relativos ao objeto da pesquisa, a montagem de uma interface-modelo capaz de rastrear e computar trajetórias de acesso de usuários, identificando isoladamente como os elementos infográficos da tela se relacionam, no contexto da recepção, à decisão do internauta pelo click-through. Esse percurso desnudou evidências da existência de fenômenos estéticos no contexto da pesquisa, fato abordado teoricamente sob a ótica do estilo, e que resultaram em proposição de uma nova abordagem para o webdesign, capaz de aproximar as visagens teóricas da cibercultura dos resultados empíricos produzidos no cotidiano. O quadro teórico da pesquisa é composto por três grandes quadrantes. No primeiro, situa-se o entendimento, no contexto das ciências sociais aplicadas, do cenário socialhistórico em que a cibercultura e seus típicos processos comunicativos tecnologicamente mediados se inserem. Nesse âmbito, figuram, como referências Zygmunt Bauman, Paul Virilio, Neil Postman, Asa Briggs e Peter Burke. Num segundo momento, é tratada a gênese das interfaces computacionais gráficas e suas metáforas, no qual a abordagem é norteada por Steven Johnson, Oliver Sacks, Philippe Breton e Walter Benjamin. Por fim, segue-se uma reflexão sobre o webdesign e suas questões estético-conceituais sob a ótica histórica do estilo e aqui as referências são Adrian Frutiger, Josef Albers, Allen Hurlburt, Donis Dondis, Charles Peirce, Eugênio Trivinho e Lucia Santaella

Genes da família homeobox têm sido alvo de intensas pesquisas científicas relacionadas ao câncer. Recentemente, mostramos que o gene homeobox TGIF1 está expresso no carcinoma epidermóide de boca na sua forma genérica. Porém, não foi feita uma discriminação entre quais variantes transcricionais, ou subtipos, do TGIF1 estariam expressas. Neste estudo, propusemo-nos a verificar diferenças na freqüência de expressão das variantes transcricionais do TGIF1 em carcinomas epidermóides de boca (CEB) em relação a tecidos morfologicamente não tumorais (TN), avaliar possíveis associações entre essas variantes nos pacientes portadores de CEB e relacionar o grau de expressão dessas variantes com aspectos clínicos, histológicos e com a sobrevida dos pacientes. Adicionalmente, procuramos analisar a expressão da proteína do TGIF1. Foram analisadas 48 amostras congeladas de CEB e 12 de TN. O RNA total de cada amostra foi extraído utilizando-se solução de TRizol®. Os transcritos do TGIF1 foram amplificados por RT-PCR para cada caso de CEB e TN utilizando-se inicialmente um par de iniciadores genéricos para todas as suas variantes. Após essa triagem, os casos positivos foram amplificados utilizando-se pares de iniciadores específicos para cada variante. Não houve diferença estatística entre a freqüência de expressão das variantes do TGIF1 nos grupos CEB e TN, porém as variantes 4 e 8 foram as que apresentaram p-valores menores. Dentro do grupo de CEB, houve associação significativa entre algumas variantes entre si (sete dos vinte e um cruzamentos), de forma que as variantes 1 e 4 foram as que mais tiverem associação com outras variantes. Dessa forma, optamos por prosseguir o estudo utilizando somente as variantes 1, 4 e 8. Não houve correlação entre a expressão das variantes selecionadas e variáveis clinicas e histológicas propostas. Em relação à sobrevida, a expressão das variantes 1 e 8 mostraram correlação univariada com o desfecho óbito, de maneira que o grupo de indivíduos que mais expressou ambas as variantes foi o que apresentou menor risco de óbito. Através da análise multivariada das variantes 1 e 8 e aspectos clínicos e histológicos, somente o tamanho da lesão e a invasão vascular sanguínea tiveram significado estatístico. A expressão protéica do TGIF1 foi analisada em 46 amostras parafinadas considerando-se a diferenciação celular, a graduação imunoistoquímica e o compartimento celular. Os resultados obtidos mostraram que as variantes do TGIF1 estão expressas diferentemente no grupo dos pacientes com CEB e sugerese que a expressão das variantes 1 e 8 estejam relacionadas, de maneira semelhante, a um menor risco de óbito para pacientes portadores de CEB. Adicionalmente, sugere-se que o tamanho da lesão e a invasão vascular sanguinea representem fatores de risco relevantes para o óbito de pacientes portadores de CEB. Sugere-se também que a expressão simultânea da proteína do TGIF1 tanto no núcleo quanto no citoplasma da célula esteja correlacionada a lesões pobremente diferenciadas. / Genes of the homeobox family have been the subject of intense scientific research related to cancer. Recently, we show that this gene is expressed in oral squamous cell carcinoma in its generic form. However, it was not made a discrimination between which transcript variants, or subtypes\", of TGIF1 were expressed. In this study, we aim to verify differences in the expression of the eight transcript variants described for the homeobox gene TGIF1 in oral squamous cell carcinomas (OSCC) related with morphologically non-tumoral tissues (NT), evaluate possible associations between these variants in patients with OSCC and relate the degree of expression of these variants with clinical, histological and survival aspects of patients. Additionally, we analyzed the expression of TGIF1 protein. It was analyzed 48 frozen samples of OSCC and 12 of NT. The total RNA from each sample was extracted using TRizol solution. TGIF1 transcripts were first amplified by RT-PCR for each case of OSCC and NT using a generic pair of primers. After these screening, the positive cases were amplified using specific pair of primers for each variant. There was no statistical difference between the frequency of expression of TGIF1 transcript variants in OSSC and NT groups, but the variants 4 and 8 had the lowest p-values. Within the OSCC group, there was a significant association between some variants among themselves (seven of the twenty-one associations), in a way that the variants 1 and 4 were the ones that had most association with other variants. Thus, we chose to continue the study using only variants 1, 4 and 8. There was no correlation between the expression of the selected variants with the clinical and histological aspects. Regarding survival, the expression of variants 1 and 8 showed statistical correlation with the outcome death, in a way that the group of patients that most expressed both variants was that one with the lower risk of death. By multivariate analysis of variants 1 and 8 and clinical and histological aspects, only the size of the lesion and vascular invasion blood were statistically significant. The protein expression of TGIF1 was analyzed in 46 paraffin-embedded tissues considering the cell differentiation, the immunohistochemistry graduation and the cell compartment. The results showed that the variants of TGIF1 are differently expressed in the OSCC group of patients and it is suggested that the expression of variants 1 and 8 may be related, in a similar way, to a lower risk of death for patients with OSCC. Additionally, it is suggested that the size of the lesion and the vascular invasion of blood represent relevant risk factors for death in patients with OSCC. It is also suggested that the simultaneous expression of TGIF1 protein not only in the nucleus but also in the cytoplasm of the cell is correlated with the poorly differentiated lesions of OSCC.

O objetivo deste trabalho é criar uma metodologia capaz de reconhecer gestos de mãos, a partir de imagens dinâmicas, para interagir com sistemas. Após a captação da imagem, a segmentação ocorre nos pixels pertencentes às mãos que são separados do fundo pela segmentação pela subtração do fundo e filtragem de cor de pele. O algoritmo de reconhecimento é baseado somente em contornos, possibilitando velocidade para se trabalhar em tempo real. A maior área da imagem segmentada é considerada como região da mão. As regiões detectadas são analisadas para determinar a posição e a orientação da mão. A posição e outros atributos das mãos são rastreados quadro a quadro para distinguir um movimento da mão em relação ao fundo e de outros objetos em movimento, e para extrair a informação do movimento para o reconhecimento de gestos. Baseado na posição coletada, movimento e indícios de postura são calculados para reconhecimento um gesto significativo. / The purpose of this paper is to develop a methodology able to recognize hand gestures from dynamic images to interact with systems. After the image capture segmentation takes place where pixels belonging to the hands are separated from the background based on skin-color segmentation and background extraction. The image preprocessing can be applied before the edge detection. The recognition algorithm uses edges only; therefore it is quick enough for real time. The largest blob from the segmented image will be considered as the hand region. The detected regions are analyzed to determine position and orientation of the hand for each frame. The position and other attributes of the hands are tracked per frame to distinguish a movement from the hand in relation to the background and from other objects in movement, and to extract the information of the movement for the recognition of dynamic gestures. Based in the collected position, movement and indications of position are calculated to recognize a significant gesture.