Valová D.: Vinca minor L. alkaloids and their biological activity III. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové, 2020, 75 p. Alzheimer's disease (AD) is a progressive, neurodegenerative disorder and it's the most common form of dementia. Because we're still not able to treat the cause of disease, searching for a new substance is relevant. This thesis is focused on isolation of alkaloids from a Vinca minor L. alkaloidal extract as a potential drug. The preparation and column chromatography of the summary extract were performed by Ing. Miroslav Ločárek as a part of his doctoral studies. Subsequent preparative TLC led to the isolation of three compounds. The chemical structures of the isolated alkaloids were elucidated by means of optical rotation, NMR and MS analyses and by comparison of the obtained data with those in the literature. One of the compounds was determined as(-)-vincine, other two compounds have not been isolated yet. Isolated compounds were also tested for their biological activity. Vincine, DV-1 a DV-3 were tested for their ability to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Additionally, vincine and DV-3 were also tested for their inhibitory activity on prolyl...

Vašíčková A.: Alkaloids of Vinca minor L. and their biological activity (inhibition of human cholinesterases) V. Diploma thesis, Charles University, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany, Hradec Králové 2020. From the selected fraction VM 215-258 were isolated 3 alkaloids by flash chromatography followed, preparative thin layer chromatography and crystallization. Their structure was determined by mass spectroscopy, NMR and optical rotation, and the obtained data were compared with those in literature. Strictamine and minovincinine belong to alkaloids previously isolated, the alkaloid AV-3 has not been isolated yet. These alkaloids were tested for their ability to inhibit human cholinesterases and their inhibitory activity was compared to standards galanthamine and physostigmine. Compound AV-3 showed mild inhibitory activity against BChE (IC50 μM > 86.3 ± 2.3), other alkaloids were considered inactive, their IC50 values against cholinesterases were > 100 μM. Key words: Vinca minor L. (Apocynaceae), vinca alkaloids, minovincinine, strictamine, Alzheimer's disease, acetylcholinesterase, butyrylcholinesterase.

An interpretive analysis of Ralph Vaughan Williams' Concerto for Bass Tuba which compares tempi, interpretation of the melodic line, ornamentation, dynamics, pitches, rhythms. phrasing and articulations as utilized by four prominent tuba performers. Performers selected to share their interpretations include Arnold Jacobs, Donald Little, Richard Nahatzki and Harvey Phillips. Little, Nahatzki and Phillips provided a copy of their solo parts with their personal markings. Jacobs gave permission to transcribe his interpretation from the recording he made with the Chicago Symphony Orchestra. Performers' biological information is included along with musical reviews of Concerto for Bass Tuba.

A minor enantiomer recycling one-pot process usingω-functionalized prochiral aldehydes as starting materials and two reinforcing catalysts has been reported. The desired aldehyde for these process studies was 5-bromo-1-pentanal. In a two-phase solvent system, enzyme-catalyzed hydrolysis of the minor enantiomer regenerates continuously the prochiral starting material and Lewis acid catalysed addition of acetyl cyanide provides the O-acetylated cyanohydrins. The minor enantiomer recycling process has been studied and improved for 5-bromo-1-pentanal to receive high enantiomeric excess and yield of the expected O-acetylated cyanohydrin.

No description available.

The city district Old Havana that is on the World Heritage List because of its Spanish colonial architecture is undergoing a restoration project of great size. The project is run by the Office of the City Historian, which in Cuban proportions, is independent the state. Instead the Office is self-financed by the profits out of the 1,5 million annual tourists that visit the already, with its 63000 inhabitants, overcrowded Old Havana.  Alongside the restoration of the deteriorated buildings, the goal is to make it without resettling the current residents. Instead the aim is to involve the citizens in the planning processes to make them participate, which is not a well-tried approach in Cuban planning. This report, based on a field study in Old Havana, investigates what methods for participative planning are used by the Office and to what extent the residents see that they have the possibility to take part. / SIDA - Minor Field Study

This report focuses on potential ways to improve the reliability of the production process at the wood refining company Dicomad S.R.L. in Santa Cruz de la Sierra, Bolivia. The main theory used has been Lean Production, as this theory suits the circumstances of the company well. Disorder has been the biggest problem, which has caused the safety in the factory to be below an acceptable level. Dicomad has two different businesses – producing decking and furniture. The decking production is fairly standardized whereas the furniture production is customized. The study was carried out during the summer and fall of 2009.The analysis has resulted in changes in the layout of the factory such that a clearer work flow as well as proper order can be maintained. More specifically, three new layout suggestions have been made. The first suggestion makes big changes in the layout creating the “optimal” layout for the current situation. The second makes slightly smaller changes and the third makes small, but important changes to the layout. Our choice of the three is the second suggestion which constitutes the best compromise given the current layout. This suggestion will bring substantial benefits in form of a clearer flow, more organized inventory and separation of the two businesses but does not include a strenuous movement of the molder. Furthermore, each operation in the factory has been scrutinized and suggested improvements have been made to banish disorganization. Suggestions include redesign of machines, addition of collecting mechanisms for material and creation of best practices for machines. The greatest improvements have been possible within the furniture manufacturing as the factory was originally designed for decking production only. Additionally, proposals about improving the light, the air quality and the general organization and cleanliness of the factory have been made.Only suggestions are presented, as the time available for the study was limited and any implementations were beyond our scope.

Magister Legum - LLM / A person should at all times try to avoid the blemish of a previous conviction and a criminal record entered against his name. A criminal record entered against his name could have detrimental consequences for an individual. An accused is usually aware of this if he is involved in a trial and is thereafter convicted and sentenced. However, the same result could occur where a person paid an admission of guilt fine. Such a person could be aware that he has attained a record, but it could also be that a payee1 of a fine is very unaware that he has attained a criminal record that is entered against his name. This study focusses on the legal consequences of the payment of such an admission of guilt fine and will endeavour to investigate the remedies available to an uninformed payee of such fines.

Domestic minor sex trafficking (DMST) is a social problem affecting children between the ages of 12 and 17 years old. The issues related to DMST present challenges for social work practitioners because they often lack knowledge regarding how to identify and provide specialized services to this population. The purpose of the qualitative study was to collect and analyze data to develop an understanding of how social workers in the northeastern region of the United States identified challenges and thereby improved their practice skills when intervening with this vulnerable population. An epistemological paradigm, with a constructivist perspective employing Nguyen's systems theory, was used to understand the phenomena. The practice-focused research question posed to 5 clinically licensed social workers (LCSW) asked about the perceived barriers hindering social work practice when identifying victims of DMST. In addition, upon recognition of DMST victims, participants described existing community services that addressed their social work practice needs. The LCSWs attended 3 hour-long audio-recorded focus groups, offering their knowledge and experiences related to DMST in the designated region. Constant comparison was used to analyze the data provided by the participants during the focus groups. The key findings indicated a lack of proper identification tools and specialized services for this community. Findings can be used to recommend social change efforts, which included increasing communication about the victims between jurisdictions and communications with policy makers and service providers regarding the need to develop and implement training on various related topics.

This study explored the lived experiences of men attracted to minors who believed they would benefit from therapy but did not seek out or attend therapeutic services; and sought to gain an understanding of how the decision to seek help or not impacted their well-being. Participants included 7 men who were recruited through the B4U-ACT online forum, which provides peer support for the minor attracted community. Participants were interviewed over Skype. Data from these interviews was analyzed and coded according to the interpretative phenomenological method as outlined by Smith et al. (2012). Five main themes emerged, providing insight as to why more therapeutic support is not sought. These themes include: emotional distress, consideration of therapy, actual and perceived stigmatization, expectations of therapist assumptions, expectations of professionalism, and therapeutic support. The findings were compared with existing literature to propose ways mental health professionals can provide easier access to resources and reach the population, which can lead to societal benefits. Improved access to treatment has the potential to provide the population opportunities to better cope with stigma, manage their impulses more effectively, lower their feelings of isolation, and increase their overall well-being. This study helps to fill the current void in the area of research on help-seeking behaviors and the associated challenges that men attracted to minors may face.