Yes / While achieving the spatial organization of cells within 3D assembled nanofiber/cell constructs via nanofiber-enabled cell layering, the small sizes of inter-fiber pores of the electrospun nanofiber mats could significantly limit cell penetration across the layers for rapid formation of an integrated tissue construct. To address this challenge, efforts were made to improve cell-infiltration of electrospun nanofiber mats by modulating the density distribution and spatial organization of the fibers during electrospinning. Collection of collagen-containing electrospun nanofibers (300–600 nm in diameter) onto the surface of a stainless steel metal mesh (1 mm × 1 mm in mesh size) led to the periodic alternation of fiber density from densely packed to loosely arranged distribution within the same mat, in which the densely packed fibers maintained the structural integrity while the region of loose fibers allowed for cell penetration. Along with improved cell infiltration, the distinct fiber organization between dense and loose fiber regions also induced different morphology of fibroblasts (stellate vs. elongated spindle-like). Assembly of cell-seeded nanofiber sheets into 3D constructs with such periodically organized nanofiber mats further demonstrated their advantages in improving cell penetration across layers in comparison to either random or aligned nanofiber mats. Taken together, modulation of nanofiber density to enlarge the pore size is effective to improve cell infiltration through electrospun mats for better tissue formation. / NSF-IIP. Grant Numbers: 1338958, 1346430; NSF-DMR. Grant Number: 1508511; NSF-CBET. Grant Number: 1033742; and NIAMS. Grant Number: 1R21 AR056416

Com o aumento do número de animais inseminados por IATF (inseminação artificial em tempo fixo), a diminuição de custos e o incremento de índices reprodutivos têm sido os objetivos de vários grupos de pesquisa. Nos protocolos de IATF utilizados, empregam-se dispositivos de liberação sustentada de progesterona (P4), que são, em sua maioria, constituídos de um esqueleto de nylon, recoberto com uma camada de silicone com P4 . Visando a diminuição dos custos de produção e impacto ambiental, foi desenvolvido um dispositivo à base de biopolímeros. Neste estudo, foram comparados dispositivos confeccionados com uma blenda constituída de Poli-hidroxi-butirato (PHB) e Poli-&epsion;-caprolactona (PCL), com o Dispositivo Interno Bovino (DIB&reg;), utilizado como controle . No teste in vitro, utilizaram-se dois tipos de dispositivo à base de biopolímeros, com uma área superficial de 147 cm2: DISP8 (46% PHB, 46% PCL e 8% P4; n=4), DISP10 (45% PHB, 45% PCL, 10% P4; n=4) e o DIB&reg; (1 g de P4, área de 120 cm2; n=3). Os testes in vitro foram conduzidos segundo especificações da USP 23, em um dissolutor de comprimidos, empregando-se uma mistura de álcool/água (60/40) como meio de difusão. Amostras do meio foram colhidas aos 2 min., 2, 4, 8, 12, 24, 48, 60, 72, 84 e 96 h. Os teores de P4 foram dosados por espectrofotometria, em 244 nm de comprimento de onda. Realizaram-se as comparações 3 a 3 dos coeficientes angulares das retas obtidas pela regressão das concentrações acumuladas de P4, em função da raiz quadrada do tempo. As médias e respectivos erros-padrão dos coeficientes angulares foram de 677,39 ± 16.13 µg/cm2/t1/2 para o DIB&reg;, 566,17 ± 3.68 µg/cm2/t1/2 para o Disp8 e 774,63 ± 45.26 µg/cm2/t1/2 para o Disp10. Houve diferença entre o DISP8 e o DISP10 (p< 0,05), mas ambos não diferiram do DIB. Para análise das quantidades liberadas por dia de teste in vitro, consideraram-se 4 períodos: 0-24, 24-48, 48-72 e 72-96 h. Houve interação entre tratamento e tempo (p=0,0024). Nas primeiras 24 horas, o DISP8 liberou significativamente menos P4 do que o DISP10 e o DIB&reg;, cujas liberações não diferiram entre si. No intervalo entre 24 e 48h, o DISP 10 liberou significativamente mais P4 do que o DIB&reg;. O DISP8 liberou uma quantidade de P4 intermediária e não se diferenciou significativamente nem do DIB&reg; e nem do DISP10. No intervalo entre 48 e 72h a quantidade de P4 liberada pelo DISP10 foi significativamente maior do que a do DIB&reg; e a do DISP8, que não diferiram entre si. No intervalo entre 72 e 96h, o DISP10 liberou significativamente mais P4 do que o DIB&reg; e o DISP8 liberou uma quantidade intermediária que não diferiu do DIB&reg; nem do DISP10. No teste in vivo, seis vacas mestiças ovariectomizadas receberam DIB (n=4) ou DISP8 (n=8), em um delineamento alternado, com seqüência não balanceada (cross-over), adicionado de medidas repetidas no tempo, referentes aos 16 dias de colheita das amostras de sangue. As amostras foram analisadas por rádio-imuno-ensaio, em fase sólida, utilizando-se &quotkit&quot comercial da DPC (Diagnostics Products Corporation). As concentrações de pico foram atingidas 4 h após a colocação dos dispositivos e este foi o único momento em que as concentrações plasmáticas de P4 diferiram segundo dispositivo (11,45±1,96 vs 9,23±1,15 ng/mL, respectivamente para DIB e DISP8; p= 0,027). No dia 8 do ensaio, as concentrações plasmáticas de P4 proporcionadas pelo DIB e pelo DISP8 foram respectivamente 2,44±0,09 e 1,89±0,13 ng/mL (p=0.58). Ambos dispositivos mantiveram teores de P4 superiores a 1 ng/mL, durante os 16 dias do teste in vivo. Concluiu-se que os dispositivos confeccionados com a blenda dos biopolímeros PHB/PCL, podem liberar P4 de maneira sustentada, tanto quanto os que empregam silicone em sua fabricação. / With the increase in the number of animals inseminated by TAI (timed artificial insemination) the decrease of costs and the improvement of reproductive performance are the goals of several research groups. Progesterone devices which are majority constituted of a nylon skeleton covered with a silicone layer with P4, are used in TAI protocols. Aiming the decrease of the production costs and the environmental impact, was a new device developed with biopolymers matrix. In this study, were compared a blend of Poli-hidroxi-butirate (PHB) and poli-?-caprolactone (PCL) device with the Bovine Internal Device (DIB&reg;). In the in vitro test, two kinds of the new device with a superficial area of 147 cm2: DISP8 (46% PHB, 46% PCL and 8% P4; n=4), DISP10 (45% PHB, 45% PCL, 10% P4; n=4) and DIB&reg; (1 g of P4, area of 120 cm2; n=3). The in vitro tests were led following USP 23 specifications in a drug dissolutor in alcohol/water (60/40) solution as a diffusion medium. Samples were collected at 2 min., 2, 4, 8, 12, 24, 48, 60, 72, 84 and 96 hours. Progesterone concentrations were estimated by spectrophotometry at UV 244 nm wavelenght. Comparisons 3 by 3 of the angular coefficients of the straight lines obtained by the regression of the accumulated P4 concentrations in function of the square root of time were performed. The averages and respective standard-errors of the angular coefficients were 677,39 ± 16.13 µg/cm2/t1/2 to DIB&reg;, 566,17 ± 3.68 µg/cm2/t1/2 to Disp8 and 774,63 ± 45.26 µg/cm2/t1/2 to Disp10. Difference were observed between DISP8 and DISP10 (p< 0.05) but both did not differ to DIB&reg;. Four periods were considered for analysis of the amounts released per day of in vitro test: 0-24, 24-48, 48-72 and 72-96 hours. Interaction between treatment and time was observed (p=0.0024). In the first 24 hours DISP8 released significantly less P4 than DISP10 and DIB&reg; but the lathers did not differ from each other. In the interval between 24 and 48 hours DISP10 released significantly more P4 than DIB&reg;. The device DISP8 released an intermediary concentration of P4 and was similar to DIB&reg; and to DISP10. In the interval between 48 and 72 hours the progesterone concentration released by DISP10 was significantly higher than DIB&reg; and DISP8 but lathers did not differ from each other. In the interval between 72 and 96 hours DISP10 released significantly more P4 than DIB&reg; while DISP8 released an intermediary amount which did not differ to DIB&reg; or to DISP10. In the test in vivo test, six ovariectomized beef cows received the devices DIB&reg; (n=4) or DISP8 (n=8) in an alternated delineation in a nom balanced sequence (cross-over) added of repeated measures in time, related to the 16 days of blood samples collects. Progesterone concentration from these samples were measured by radioimmunoassay in solid phase using the commercial kit from DPC (Diagnostics Products Corporation). The peak of the progesterone concentrations were observed 4 hours after the devices insertion and this was the unique moment in which plasmatic progesterone concentrations differed depending on device (11,45 ± 1,96 vs 9,23 ± 1,15 ng/mL, respectively for DIB and DISP8; p= 0,027). After 8 days from the device insertion plasmatic progesterone concentrations provided by DIB and DISP8 were respectively 2,44 ± 0,09 and 1,89 ± 0,13 ng/mL (p=0.58). Both devices kept the progesterone concentration higher than 1 ng/mL during the 16 days of the in vivo test. In conclusion, the devices made with biopolymers blends of PHB/PCL can release sustained P4 comparable with silicone matrix.

Submitted by admin tede (tede@pucgoias.edu.br) on 2018-06-20T17:56:44Z No. of bitstreams: 1 Julia Nunes de Souza Teixeira.pdf: 1102437 bytes, checksum: ba92bf6daf45c58fbe96e7ba4c524dab (MD5) / Made available in DSpace on 2018-06-20T17:56:44Z (GMT). No. of bitstreams: 1 Julia Nunes de Souza Teixeira.pdf: 1102437 bytes, checksum: ba92bf6daf45c58fbe96e7ba4c524dab (MD5) Previous issue date: 2017-10-26 / This dissertation is organized into two articles that aim to to understand psychopathic traits in sex offender against children and adolescents, as well as their relationship with the experience of victimization throughout life. The first article aims to systematically explore and synthesize the knowledge produced about the use of Psychopathy Checklist-Revised - PCLR in sex offender against children and adolescents. It was found a total of 19 articles through which it was possible to verify that PCL-R, in most cases, can be considered a useful and valid instrument to assess the psychopathy in SVA in the cultural contexts in which the instrument was applied, as well as as to predict criminal recidivism in this specific sample. The second article is an empirical study, whose main objective was to investigate the personality traits of SVA through PCL-R and the victimizations suffered by them through the Juvenile Victimization Questionnaire - JVQ. Thirty SVA, male volunteers, serving a prison sentence in a state penitentiary in the state of Goiás, were divided into two groups: G1 AVS considered psychopathic (PCL-R≥30 points) and G2 (SVA (PCL-R <30) not considered psychopathics .The results indicated that 33.3% of the sample met the criteria for psychopathy (score> 30 in PCL-R). The results showed significant differences between groups: the younger the SVA, the greater the PCL-R score; the higher the PCL-R score, the greater the variety of crimes committed, the greater the number of victims, the more escapes and rebellions, the greater the number of cases responding and the longer the total time of punishment. The results also indicated a positive correlation between the PCL-R score and G1 malpractice, revealing that the higher the PCL-R score, the greater the number of victimizations suffered in the maltreatment screening. / A presente dissertação de mestrado está organizada em dois artigos que têm como objetivo compreender os traços de psicopatia em autores de violência sexual contra crianças e adolescentes (AVS), assim como a relação desses traços com a vivência de vitimização ao longo da vida. O primeiro artigo tem como objetivo explorar e sintetizar o conhecimento produzido sobre o uso do Psychopathy Checklist-Revised - PCL-R em autores de violência sexual. Foi encontrado um total de 19 artigos por meio dos quais foi possível constatar que o PCL-R, na maioria dos casos, pode ser considerado um instrumento útil e válido para avaliar a psicopatia em AVS nos contextos culturais em que o instrumento foi aplicado, bem como para prever a reincidência criminal nessa amostra específica. O segundo artigo trata de um estudo empírico, cujo objetivo principal foi investigar os traços de personalidade de AVS por meio do PCL-R e as vitimizações sofridas por eles por meio do Juvenile Victimization Questionnaire - JVQ. Participaram do estudo 30 AVS, voluntários, do sexo masculino, cumprindo pena em regime fechado em uma penitenciária do estado de Goiás, que foram subdivididos em dois grupos: G1 AVS considerados psicopatas (PCL-R³ 30 pontos) e o G2 (AVS não considerados psicopatas (PCL-R< 30). Os resultados indicaram que 33,3% da amostra preenchiam os critérios para psicopatia (pontuação > 30 no PCL-R). Os resultados mostraram diferenças significativas entre os grupos: quanto mais jovem o AVS maior foi a pontuação no PCL-R; quanto mais elevada a pontuação no PCL-R, maior a variedade de crimes cometidos, maior o número de vítimas, mais fugas e rebeliões, maior o número de processos que respondiam e maior o tempo total de pena. Os resultados apontaram também correlação positiva entre a pontuação no PCL-R e maus tratos para o G1, revelando que quanto maior a pontuação no PCL-R, maior o número de vitimizações sofridas no crivo maus tratos.

Bei überkritisch großen Läsionen des peripheren Nervensystems, die zum Verlust von Motorik bzw. Sensibilität an Extremitäten führen und damit eine erhebliche Beeinträchtigung der Lebensqualität des Patienten bedingen, ist der Einsatz von Überbrückungsstrukturen bzw. Nervenleitkanälen notwendig. Da weder autologe Transplantate noch künstliche Konstrukte im klinischen Alltag bislang zufriedenstellende Ergebnisse lieferten, ist die Nachfrage nach alternativen Materialien und Konzepten des Tissue Engineering hoch. Im Rahmen dieser Dissertation ist es gelungen zwei thermoplastische Methoden zu etablieren, mit denen aus dem im medizinischen Sinne interessanten und relativ neuartigen Material Poly(3-hydroxybuttersäure) in Kombination mit Polycaprolacton poröse resorbierbare Hohlstrukturen bzw. Hohlfasern erzeugt werden können, die den hohen aktuellen Anforderungen an eine Nervenleitschiene gerecht werden. Neben der Entwicklung und Charakterisierung sowie Modifizierung der erzeugten Leitkanäle bezüglich Porosität, Permeationsverhalten, mechanische Eigenschaften und Oberflächenfunktionalisierung, konnten strukturelle als auch biochemische Reize in diese integriert werden, die in einer Reihe von ex-vivo Studien mit neuronalen Primärzellen hinsichtlich Adhäsion, Vitalität und Ausbreitungsverhalten untersucht werden konnten. Es konnte eine Art „Toolbox“ aus PHB basierten Strukturen erstellt werden, die es erlaubt hierarchische Strukturen zusammenzustellen, die entsprechend des peripheren Defektes zusammengesetzt und biomolekular maßgeschneidert werden könnten, um die native Struktur bestmöglich temporär bis zur vollständigen Regeneration zu imitieren und damit die Therapie größerer Defekte zu ermöglichen bzw. die als Plattform für weitere Konzepte der Grundlagenforschung im Bereich des Neuro-Tissue Engineering dienen.

Les polymères en étoile connaissent un intérêt accru en raison de leurs propriétés thermiques et mécaniques inimitables. Partant du constat qu’en parallèle la chimie durable se développe à un rythme sans précédent, nous proposons dans cette thèse de développer une stratégie plus « verte » pour la synthèse de polymères en étoile de type D-sorbitol-poly(ε-caprolactone) (star PCL-OHx). Ces derniers seront synthétisés sans solvant (en masse) ou dans des solvants « propres » (CO2 supercritique) et en présence du catalyseur métallique Sn(Oct)2 (qui a été approuvé par la FDA) ou d’un catalyseur enzymatique (Novozym® 435). L’influence de ces paramètres sur l’architecture des étoiles (nombre de bras, masse molaire des bras…) a été rigoureusement analysée et confirmée par différentes techniques d’analyse (RMN 1H, SEC-MALS, SEC-UC, analyse RMN 31P quantitative via la méthode de phosphitylation). Des polymères linéaires monohydroxy (PCL-OH) et téléchéliques dihydroxy (OH-PCL-OH) ont également été synthétisés en parallèle. Ces différentes PCL ont été utilisées comme macroamorceurs hydrophobes pour la polymérisation du monomère cyclique hydrophile éthyl phosphonate d’éthylène. Une large gamme de copolymères amphiphiles a ainsi pu être développée (i.e. des copolymères diblocs en étoile ou des copolymères diblocs et triblocs linéaires). Le comportement d’auto-assemblage en solution de ces copolymères a été étudié par DLS ainsi que par TEM et cryo-TEM. Nous avons également montré que les copolymères amphiphiles triblocs et en étoile sont capables de diminuer la tension de surface (γ) de l’eau en dessous de 45 mN m-1. Enfin, les étoiles PCL-OHx synthétisées en présence d’enzyme ont été fonctionnalisées par réaction avec l’anhydride maléique. Des polymères en étoile, ioniques, tensio-actifs et directement dispersables dans l’eau ont ainsi pu être obtenus. Dans un second temps, ces mêmes polymères ont été photopolymérisés en présence d’une faible quantité de tri(éthylène glycol) divinyl éther (~ 9% par rapport à la masse totale). Les films afférents à ces copolymères en étoile réticulés sous UV ont été analysés par IRTF, DSC et ATG. / Star polymers have attracted considerable attention because of their unique thermal and mechanical properties. At the same time, as sustainable chemistry field is growing in impact at an unprecedented rate, we propose in this work to implement a greener pathway for the synthesis of star D-sorbitol-poly(ε-caprolactone) (star PCL-OHx) using clean solvents (polymerisation in the bulk or in supercritical CO2) and either FDA-approved Sn(Oct)2 catalyst or enzyme catalyst (Novozym® 435). The influence of these parameters on the star architecture (number of arms, MW of arms etc.) was rigorously analysed and corroborated with various analytical techniques (1H NMR, SEC-MALS, SEC-UC, phosphitylation quantitative 31P NMR approach). Linear monohydroxy PCL-OH and dihydroxy telechelic OH-PCL-OH samples were also prepared. The PCL materials obtained were used as hydrophobic macroinitiators for the polymerisation of cyclic hydrophilic ethylene ethyl phosphonate monomer for the synthesis of a range of different amphiphilic materials (i.e. star diblock, linear diblock and triblock copolymers). Self-assembly behaviour in aqueous solution of these copolymers was investigated by DLS, TEM and cryo-TEM. Triblock and star amphiphilic copolymers were revealed to be able to reduce the surface tension (γ) of water down to 45 mN m-1. Finally, enzyme catalysed star PCL-OHx polymers were functionalised with carboxylic end-groups using maleic anhydride. Water-dispersible surface-active ionic star polymers were then obtained. These maleate-functionalised star polymers were then photopolymerised with a small amount of tri(ethylene glycol) divinyl ether (~9wt% of total composition). The UV-cured crosslinked star PCL films produced were then analysed by FTIR, DSC and TGA.

MORANA, HCP. Identificação do ponto de corte para a escala PCL-R (Psychopathy Checklist Revised) em população forense brasileira : caracterização de dois subtipos de personalidade ; transtorno global e parcial. São Paulo, 2003. 178p. Tese (Doutorado) – Faculdade de Medicina, Universidade de São Paulo. No Brasil, não existem até o momento, na esfera jurídico-penal, instrumentos padronizados que permitam a identificação de sujeitos que possam vir a reincidir em crimes, principalmente os de natureza violenta e cruel. O PCL-R, já amplamente utilizado e institucionalizado em diversos países, preenche esta lacuna. Este estudo visa discriminar, através da análise da personalidade, sujeitos considerados psicopatas e não psicopatas, correlacionados neste estudo como transtorno global (TG) e parcial (TP) da personalidade, respectivamente. O grupo de estudo é composto por 56 sujeitos denominados de POPULAÇÃO FORENSE, sendo: 33 sujeitos identificados como TG e 23 sujeitos identificados como TP. Todos os 56 sujeitos foram analisados através dos dados clínicos, institucionais, da Prova de Rorschach e do PCL-R. Esta população foi comparada a 30 sujeitos denominados de POPULAÇÃO CONTROLE, e que também foram avaliados pelos mesmos instrumentos do grupo de estudo. A personalidade, de ambos os grupos da população forense, se relaciona a condutas anti-sociais, mas apresenta diferenças quanto à dinâmica psíquica e às repercussões sobre o comportamento. A correta identificação de tais sujeitos apresenta implicações diversas para o sistema forense e penitenciário e pode vir a contribuir para diminuir os índices de reincidência criminal e de violência no país. A validação do instrumento (PCL-R) e identificação do ponto de corte para a condição de psicopatia (TG em nossa notação) foi realizada através do uso concorrente entre ambos os instrumentos e resultou no valor de 23 (IC=21,61 a 24,05). A média encontrada foi de 28,63 (IC=26,78 a 30,48). O valor da medida da sensibilidade foi de 84,8% e a confiabilidade entre ambos os instrumentos, medida através do índice Kappa, foi considerada elevada, resultando no valor de 0,8735. / MORANA, HCP. Identifying the Brazilian PCL-R (Psychopathy Checklist Revised) cut-off score for forensic population : characterizing two personality subtypes; global personality disorder (GPD) and Partial personality disorder (PPD). São Paulo, 2003. 178p. Tese (Doutorado) – Faculdade de Medicina, Universidade de São Paulo. In Brazil, there have been no standardized instruments in the legal and penal field so far that enable-identifying subjects who may relapse into crime, mainly those crimes of violent and cruel nature. The PCL-R, broadly used and institutionalized in several countries, fills such gap. The goal of the present study is to discriminate, through in-depth personality assessment, subjects considered psychopathic and non-psychopathic, correlated in this study to individuals specified as having global personality disorder (GPD) and partial personality disorder (PPD), respectively. The sample is composed of a random sample of 56 male subjects referred to as forensic population, these being: 33 subjects identified as GPD and 23 subjects identified as PPD. All 56 subjects have been assessed on the light of clinical data, judicial records, the Rorschach Test and the PCL-R. This population was compared to 30 subjects referred to as non-criminal population, and who have also been assessed via the same instruments as the sample. The personality of both forensic population groups fulfills ICD-10 criteria for antisocial personality disorder, but differences in terms of their psychic dynamics and personality determinants can be clearly identified, which impacts upon their social behavior in different ways. The accurate identification of such subjects presents multiple implications to the forensic and penitentiary system, and may contribute to reduce recidivism rates as well as violence levels in the country. Validation of the instrument (PCL-R) and identification of the cut-off score for the psychopathic condition (GPD in our notation), for the sample drawn from the Brazilian forensic population has been conducted through the concurrent analysis of both instruments of assessment, resulting in a 23 cut-off score (CI = 21.61 to 24,05). The average value is 28,63 (CI= 26,78 to 30, 48). Sensitivity outcome is 84,8%. Specificity is 100%. The reliability rates found for both instruments were high (Kappa = 0.8735).

MORANA, HCP. Identificação do ponto de corte para a escala PCL-R (Psychopathy Checklist Revised) em população forense brasileira : caracterização de dois subtipos de personalidade ; transtorno global e parcial. São Paulo, 2003. 178p. Tese (Doutorado) – Faculdade de Medicina, Universidade de São Paulo. No Brasil, não existem até o momento, na esfera jurídico-penal, instrumentos padronizados que permitam a identificação de sujeitos que possam vir a reincidir em crimes, principalmente os de natureza violenta e cruel. O PCL-R, já amplamente utilizado e institucionalizado em diversos países, preenche esta lacuna. Este estudo visa discriminar, através da análise da personalidade, sujeitos considerados psicopatas e não psicopatas, correlacionados neste estudo como transtorno global (TG) e parcial (TP) da personalidade, respectivamente. O grupo de estudo é composto por 56 sujeitos denominados de POPULAÇÃO FORENSE, sendo: 33 sujeitos identificados como TG e 23 sujeitos identificados como TP. Todos os 56 sujeitos foram analisados através dos dados clínicos, institucionais, da Prova de Rorschach e do PCL-R. Esta população foi comparada a 30 sujeitos denominados de POPULAÇÃO CONTROLE, e que também foram avaliados pelos mesmos instrumentos do grupo de estudo. A personalidade, de ambos os grupos da população forense, se relaciona a condutas anti-sociais, mas apresenta diferenças quanto à dinâmica psíquica e às repercussões sobre o comportamento. A correta identificação de tais sujeitos apresenta implicações diversas para o sistema forense e penitenciário e pode vir a contribuir para diminuir os índices de reincidência criminal e de violência no país. A validação do instrumento (PCL-R) e identificação do ponto de corte para a condição de psicopatia (TG em nossa notação) foi realizada através do uso concorrente entre ambos os instrumentos e resultou no valor de 23 (IC=21,61 a 24,05). A média encontrada foi de 28,63 (IC=26,78 a 30,48). O valor da medida da sensibilidade foi de 84,8% e a confiabilidade entre ambos os instrumentos, medida através do índice Kappa, foi considerada elevada, resultando no valor de 0,8735. / MORANA, HCP. Identifying the Brazilian PCL-R (Psychopathy Checklist Revised) cut-off score for forensic population : characterizing two personality subtypes; global personality disorder (GPD) and Partial personality disorder (PPD). São Paulo, 2003. 178p. Tese (Doutorado) – Faculdade de Medicina, Universidade de São Paulo. In Brazil, there have been no standardized instruments in the legal and penal field so far that enable-identifying subjects who may relapse into crime, mainly those crimes of violent and cruel nature. The PCL-R, broadly used and institutionalized in several countries, fills such gap. The goal of the present study is to discriminate, through in-depth personality assessment, subjects considered psychopathic and non-psychopathic, correlated in this study to individuals specified as having global personality disorder (GPD) and partial personality disorder (PPD), respectively. The sample is composed of a random sample of 56 male subjects referred to as forensic population, these being: 33 subjects identified as GPD and 23 subjects identified as PPD. All 56 subjects have been assessed on the light of clinical data, judicial records, the Rorschach Test and the PCL-R. This population was compared to 30 subjects referred to as non-criminal population, and who have also been assessed via the same instruments as the sample. The personality of both forensic population groups fulfills ICD-10 criteria for antisocial personality disorder, but differences in terms of their psychic dynamics and personality determinants can be clearly identified, which impacts upon their social behavior in different ways. The accurate identification of such subjects presents multiple implications to the forensic and penitentiary system, and may contribute to reduce recidivism rates as well as violence levels in the country. Validation of the instrument (PCL-R) and identification of the cut-off score for the psychopathic condition (GPD in our notation), for the sample drawn from the Brazilian forensic population has been conducted through the concurrent analysis of both instruments of assessment, resulting in a 23 cut-off score (CI = 21.61 to 24,05). The average value is 28,63 (CI= 26,78 to 30, 48). Sensitivity outcome is 84,8%. Specificity is 100%. The reliability rates found for both instruments were high (Kappa = 0.8735).

This master’s thesis deals with lead acid battery in hybrid electric vehicles which operating at mode of partial state of charge (PSoC). In this mode, there is often a negative electrode degradation mostly due to irreversible mechanism of suphation. The idea of this work is detect ideal value of mechanical pressure exerted on active mass of negative electrode in combination with additives to prevent mechanism of sulphation and lead to increased cycle life of lead acid battery.

Abstrakt Bakgrund: Posttraumatiskt stressyndrom (PTSD) innebär en förlängd stressreaktion och beteendeförändring hos en person. Riskfaktorer på IVA för utvecklandet av PTSD är bland andra vårdmiljö, läkemedel, lång vårdtid samt mekanisk ventilation. COVID-19 kan orsaka följdsjukdomar, såväl fysiska- som psykiska, vilket också innebär en risk för dessa patienter att utveckla PTSD.  Motiv: Pandemin har inneburit en extrem belastning på intensivvården. Det är känt att patienter som vårdas på IVA löper risk att utveckla PTSD. Förekomst av symtom på PTSD efter allvarlig COVID-19 infektion är ännu relativt outforskat.  Syfte: Syftet med studien var att kartlägga förekomst av symtom på PTSD efter allvarlig COVID-19 infektion som krävt intensivvård.  Metod: Föreliggande studie har tagit del av data från SWECRIT COVID-19 studien som baseras på kritiskt svårt sjuka patienter med COVID-19 som intensivvårdats i Region Skåne. Kvantitativ ansats med en icke experimentell design var lämpligt, eftersom skattningsinstrumentet PCL-5 användes. Urvalet i studien skedde konsekutivt. Totalt deltog 174 patienter i studien. Variabler som kön, ålder, civilstatus, modersmål och utbildningsnivå studerades. Data analyserades i Jamovi (version 1.6.23).  Resultat: Resultatet visade att 16% av patienterna uppnådde tröskelvärdet ≥33 poäng för symptom på PTSD. Patienter med annat modersmål än svenska visade sig skatta högre för symptom på PTSD, sambandet var signifikant (p=0.003). Kvinnor skattade högre för symtom på PTSD än män, men den procentuella fördelningen bland de som uppfyllde tröskelvärdet var lika mellan könen.  Konklusion: Personcentrerad vård och förebyggande arbete kan ha betydelse för förekomst av symtom på PTSD. Att förhindra eventuella språkbarriärer hos patienter med annat modersmål är av vikt. Fortsatta uppföljningsstudier vid 12- och 36-månader kan visa hur förekomsten av PTSD utvecklas över tid. Kompletterande kvalitativ forskning kan nyansera patienters unika upplevelse av intensivvård för COVID-19. / Abstract  Background: Post-traumatic stress disorder (PTSD) involves a prolonged stress response and behavioral change in a person. Risk factors for the development of PTSD in ICU are care environment, drugs, prolonged care and mechanical ventilation. COVID-19 can cause physical and mental sequelae, which also put these patients at risk for developing PTSD. Motive:  The pandemic has put an extreme strain on intensive care. It is known that patients in ICU are at risk of developing PTSD. The incidence of PTSD symptoms after severe COVID-19 infection is still relatively unexplored. Aim: The aim of the study was to examine the occurrence of symptoms of PTSD after severe COVID-19 infection that required intensive care. Method: The present study has taken part of data from the SWECRIT COVID-19 study, which is based on critically ill patients with COVID-19 who received intensive care in Skåne, Sweden. Quantitative approach with a non-experimental design was appropriate since the instrument PCL-5 was used. The selection of patients was made consecutively. A total of 174 patients participated in the study. Variables such as gender, age, marital status, language and level of education were studied. Data were analyzed in Jamovi (version 1.6.23). Result: The results showed that 16% of the patients reached the threshold ≥33 points for symptoms of PTSD. Patients with other languages than Swedish were found to rate higher for symptoms of PTSD, the association was significant (p = 0.003). Women rated higher for symptoms of PTSD than men, but the distribution of percentage among those who met the threshold was equal between the genders.  Conclusion: Person-centered care and preventive work can be important for the prevalence of symptoms of PTSD. Preventing language barriers are important. Follow-up studies at 12 and 36 months can show how the prevalence of PTSD develops over time. Complementary qualitative research can nuance patients' unique experience of intensive care for COVID-19.

L’objectif de cette thèse était d’allier, dans un même polymère, le contrôle de l’architecture macromoléculaire, la fonctionnalité et la possibilité de sa mise en oeuvre en phase fondue, tout en préservant l’aspect environnemental. Les structures polymères synthétisées sont basées sur des chaînes biodégradables et/ou biosourcées d’acide polylactic (PLA), de polyhydroxybutyrate (PHB) et de polycaprolactone (PCL). Ces dernières ont été assemblées dans des structures macromoléculaires branchées à design contrôlé et portant des fonctions thiols, ces fonctions ont permis le greffage de monomères dotés de groupements ammoniums quaternaires, sur les structures obtenues, via une addition radicalaire thiol-ène.Les produits obtenus ont été mélangés en phase fondue, par extrusion, avec des matrices de PLA et de PCL, pour préparer des films. Ces derniers ont fait l’objet d’une étude d’activité antibactérienne qui a montré une grande efficacité envers différents types de bactéries / The aim of this work was to develop polymers that combine controlled macromolecular architectures, functionality, melt processing and an environmentally friendly aspect. The obtained polymeric structures were based on biodegradable and/or biosourced chains of polylactic acid (PLA), polycaprolactone (PCL) and polyhydroxybutyrate (PHB). The lasts were assembled in branched macromolecular structures with controlled design and bearing thiol functions, these functions allowed the grafting of quaternary ammoniumcontaining monomers on the branched structures according to a thiol-ene radical addition mechanism. The final products were blended with neat matrices of PLA and PCL in the melt state, by extrusion process, to make polymeric films. The obtained film-shaped blends were subjected to antibacterial activity study showing there high efficiency against different types of bacteria