Global ETD Search

81	Detecção de adutos de trans,trans-2,4-decadienal em citocromo c. Efeitos em mitocôndrias isoladas / Detection of trans,trans-2,4-decadienal adducts in cytochrome c. Effects on isolated mitochondrial functions Carlos Alexandre Oliveira Sigolo 28 September 2007 (has links) A atividade biológica de aldeídos α,Β-insaturados tem sido associada a diversos processos incluindo regulação gênica, envelhecimento Alzheimer e disfunções mitocondriais. Neste trabalho investigamos a formação de adutos do trans,trans-2,4-decadienal (DDE), um aldeído produzido endogenamente e presente como contaminante em alimentos e água, em lisina, histidina e citocromo c. Avaliamos também alterações na função de mitocôndrias de fígado de rato expostas ao aldeído. As análises por espectrometria de massas, LC-ESI/MS, indicaram a formação de diversos tipos de adutos de DDE nos aminoácidos lisina e histidina, entre eles bases de Schiff e enaminas. Os resultados obtidos por espectrometria de massas, MALDI-Tof, indicaram a formação de adutos de DDE formados via base de Schiff de maneira concentração do aldeído, tempo e pH dependentes. As análises da proteína digerida por ESI-Q-ToF, indicaram que os adutos foram formados nos resíduos H-33, K-39, 72 e 100, localizados em regiões ricas em resíduos básicos, cuja interação com membranas e citocromo e oxidase tem sido postulada. Observamos também o deslocamento da banda Soret (λ = 409 nm) e o desaparecimento da banda em λ = 695 nm, relativa a coordenação do sexto ligante do grupo heme (M-80). Este fenômeno está associado a abertura da cavidade do grupo heme e o deslocamento do ligante, indicando alterações nas estruturas secundária e terciária da proteína. Os experimentos realizados com mitocôndrias isoladas indicaram que DDE promove danos à membrana interna mitocondrial, demonstrando i pelo aumento no consumo de O2 em mitocôndrias em estado 4. Em decorrência destas lesões observamos também o intenso inchamento mitocondrial, indicado por experimentos de espalhamento de luz e microscopia eletrônica de transmissão. O inchamento não foi bloqueado por ciclosporina A, um inibidor do poro de transição mitocondrial. DDE também induziu a perda do potencial de membrana da organela, demonstrado pelo monitoramento do indicador fluorescente safranina O e aumento da peroxidação lipídica atestado pela quantificação de malondialdeido (MDA). Estes resultados indicam que DDE promove alterações estruturais no citocromo e podendo levar ao comprometimento da atividade da proteína, além de promover alterações em parâmetros mitocondriais, indicando um possível envolvimento na disfunção mitocondrial promovida por estresse oxidativo. / Lipid hydroperoxide-derived α,β-unsaturated aldehydes are involved in several cellular processes such as gene expression, aging, Alzheimer disease and mitochondrial dysfunction. In this work we have investigated adduct formation in lysine, histidine and cytochrome c by trans,trans-2,4-decadienal (DDE), an endogenously lipoperoxidation product. DDE is also a widespread environment aldehyde found, for example, in food and as a contaminant in water. Alterations in rat liver mitochondrial parameters such as oxygen consumption, membrane potentials, swelling and lipoperoxidation were also investigated. LC-ESI/MS experiments indicated that DDE react with aminoacids lysine and histidine producing adducts. In addition, MALDI-TOF experiments indicated increases in the molecular weight of cytochrome c consistent with the formation of DDE adducts via the Schiff base mechanism. Our data shows that the protein modification was time, pH and DDE-concentration dependent, leading to the formation of at least six adducts after 2 h incubation at pH 7.4. ESI-Q-TOF MS analysis of tryptic digests indicated that H-33, K-39, K-72 and K-100 were modified by DDE. These adducts are present in clusters of basic amino acid residues, which are believed to participate in the interaction of cytochrome c with mitochondrial membrane and cytochrome c oxidase. A blue shift in the Soret band from 409 to 406 nm was also observed, indicating heme crevice opening and displacement of heme sixth ligand (Met-80) coordination in modified protein. DDE (180 µM) treatment increases the rate of mitochondrial oxygen consumption, suggesting a partial mitochondrial uncoupling. Moreover, extensive mitochondrial swelling upon treatment with DDE (900 nM-162 &#181M) was observed by light scattering and transmission electron microscopy experiments. These effects were not prevented by the mitochondrial permeability transition inhibitor cyclosporin A. A DDE concentration-dependent loss in the inner mitocondrial membrane potential, monitored by safranin O fluorescence and an increase in lipoperoxidation were also observed. All together, these results suggest that reactive aldehydes can induce inner mitochondrial membrane damage playing a role in mitochondrial dysfunction associated with oxidative stress. Citocromo c DDE Desacoplamento mitocôndrial Espécies reativas de oxigênio Histidina Lisina Radical livre Cytochrome c DDE Histidine Lysine Mitochondrial uncoupling Reactive oxygen species
82	Le trouble de stress post traumatique, une pathologie de la réactivation mnésique ? Recherche d'un découplage monoaminergique et de nouvelles tentatives thérapeutiques chez le rat / Post-traumatic stress disorder, a pathology of memory reactivation? In search for monoaminergic uncoupling and new therapeutic approaches on rats Le Dorze, Claire 12 December 2016 (has links) Le Trouble de Stress Post-Traumatique (TSPT) est une pathologie qui se développe chez des sujets exposés à des événements traumatiques. Cette pathologie est caractérisée par des reviviscences du traumatisme induisant des troubles anxieux invalidants et durables. Ces reviviscences, provoquées par des indices de rappel, sont à l'origine des fréquentes rechutes qui caractérisent le TSPT. La dépendance aux drogues d'abus est également caractérisée par une hyperréactivité aux indices de rappel qui est responsable du désir irrépressible de drogue ou " craving " et des nombreuses rechutes après abstinence. Nous avons fait l'hypothèse que cette susceptibilité aux indices environnementaux, commune aux deux pathologies, pourrait être due à un découplage des systèmes monoaminergiques induit par l'exposition à des conditions intenses, drogues d'abus ou traumatisme. Les données de cette thèse montrent que notre modèle animal de traumatisme (le Single Prolonged Stress) reproduit chez les individus vulnérables les symptômes de la pathologie, et une réactivité aux indices de rappel. Nos données indiquent également qu'un traumatisme induit, chez les individus vulnérables, une désensibilisation comportementale et une sensibilisation noradrénergique corticale, supportant l'hypothèse de découplage monoaminergique. Enfin, nous avons développé une nouvelle approche thérapeutique, le " remodelage émotionnel " capable de diminuer durablement les symptômes de type TSPT. Les résultats obtenus dans cette thèse, soutiennent l'hypothèse de bases physiologiques communes entre le TSPT et l'addiction, et proposent de nouvelles approches thérapeutiques pour ces deux pathologies. / Post-Traumatic Stress disorder (PSTD) appears on a part of individuals exposed to traumatic events. This pathology is characterized by frequent re-experiencing of the traumatic event inducing disabling and long-lasting anxiety disorders. These flashbacks, triggered by reminder cues, are responsible for the frequent relapses that characterize PTSD. Addiction to drugs of abuse is also characterized by a hyper reactivity to reminder cues which is responsible for drug craving and relapses. We hypothesized that such a susceptibility to environmental cues, common to both pathologies, could be due to an uncoupling of monoaminergic systems induced by exposure to intense conditions (trauma or drugs). Data from this thesis showed that our animal model of PTSD (the Single Prolonged Stress) reproduced PTSD-like symptoms on vulnerable rats, and reactivity to reminder cues. Our data also showed that trauma induced a behavioral desensitization and a cortical noradrenergic sensitization, in vulnerable traumatized rats, supporting the hypothesis of monoaminergic uncoupling. Finally, we developed a new therapeutic approach, the "emotional remodeling" which was shown to durably decrease PTSD-like symptoms. The results obtained in this thesis support the hypothesis of common physiological basis between PTSD and drug addiction, and offer new therapeutic approaches for these two pathologies. Trouble de stress post-traumatique Addiction Découplage monoaminergique Sensibilisation noradrénergique Remodelage émotionnel Modèle animal Post-traumatic stress disorder (PTSD) Monoaminergic uncoupling Noradrenergic sensitization 573.86
83	The Structural Characterization of Two Prokaryotic Membrane Proteins: CfrA and ELIC Carswell, Casey January 2014 (has links) This thesis focuses on the structural and functional characterization of two integral membrane proteins; CfrA, an outer membrane TonB-dependent transporter (TBDT) from Campylobacter jejuni, and ELIC, a pentameric ligand-gated ion channel (pLGIC) from Erwinia Chrysanthemi. The spectroscopic characterization of CfrA revealed a fold consistent with the structural and biophysical properties observed for other TBDT. Both a homology model of CfrA and sequence alignments of CfrA with other ferric-enterobactin transporters suggested a unique mode of ligand binding, thus raising the possibility that C. jejuni can be specifically inhibited. To investigate the molecular determinates of binding to CfrA, I set out to crystallize CfrA. Hundreds of crystal trials led to crystals diffracting to 3.6 Å resolution, with a complete data set acquired at 5 Å resolution that led to a structural model of the CfrA β-barrel. In the second part of this thesis, I reconstituted ELIC into model membranes in order to test the role of intramembrane aromatic interactions in ELIC gating and lipid sensing. ELIC was reconstituted into both asolectin (aso-ELIC) and 1-palmitoyl-2-oleoyl phosphatidylcholine (PC-ELIC), membranes that stabilize the homologous nicotinic acetylcholine receptor (nAChR) in functional coupled versus non-functional uncoupled conformations, respectively. In both membrane environments, ELIC exhibits a mixed α-helical and β-sheet secondary structure, with a thermal denaturation intermediate between those of the nAChR and the close prokaryotic homolog, GLIC, in similar membranes. The data suggest that although ELIC has a decreased propensity to adopt an uncoupled conformation relative to the nAChR, its ability to undergo cysteamine-induced channel gating is sensitive to its lipid environment. The decreased propensity to uncouple may reflect an increased level of aromatics at the interface between the transmembrane α-helices, M1, M3, and M4. To test this hypothesis further, the level or aromatic residues at the M1, M3, and M4 interface in both GLIC and ELIC were varied, and in both cases the levels of intramembrane aromatic interactions correlated with the efficiency of coupling binding to gating. The data provide further evidence for a role of intramembrane aromatics in channel gating and in dictating the propensity of pentameric ligand-gated ion channels to adopt an uncoupled conformation. Membrane Protein Campylobacter jejuni Crystallization FTIR TonB Dependent Transporter CfrA ELIC Pentameric Ligand Gated Ion Channels Uncoupling structural characterization lipid sensitivity coupling aromatics Intramembrane aromatics
84	1α,25-Dihydroxyvitamin D<sub>3</sub> Reverses Nitric Oxide and Peroxynitrite Imbalance in Dysfunctional Endothelium: A Nanomedical Approach Khan, Alamzeb 21 September 2015 (has links) No description available. Biochemistry 1a,25-Dihydroxyvitamin D3 Nitric oxide and Peroxynitrite Imbalance Dysfunctional Endothelium
85	Sobre a existência de soluções estacionárias para um sistema de reação-difusão. / About the existence of stationary solutions for a reaction-diffusion system. VIEIRA, Francisca Leidmar Josué. 22 July 2018 (has links) Submitted by Johnny Rodrigues (johnnyrodrigues@ufcg.edu.br) on 2018-07-22T14:08:51Z No. of bitstreams: 1 FRANCISCA LEIDMAR JOSUÉ VIEIRA - DISSERTAÇÃO PPGMAT 2009..pdf: 290173 bytes, checksum: 21a058b9a6d5dfdd80b44bb2f900d25f (MD5) / Made available in DSpace on 2018-07-22T14:08:51Z (GMT). No. of bitstreams: 1 FRANCISCA LEIDMAR JOSUÉ VIEIRA - DISSERTAÇÃO PPGMAT 2009..pdf: 290173 bytes, checksum: 21a058b9a6d5dfdd80b44bb2f900d25f (MD5) Previous issue date: 2009-03 / Capes / O resumo foi escrito utilizando formulas e equações matemáticas que não fora possíveis serem transcritas aqui. Para a visualizar o resumo recomendamos o downloado do arquivo. / The abstract was written using mathematical formulas and equations that could not be transcribed here. To view the summary we recommend downloading the file. Matemática. Sistema de reação-difusão Soluções estacionárias Princípio do Máximo Simetria de soluções Desacoplagem linear Método de subsolução e supersolução Problema superlinear Teorema de ponto fixo Teorema de Krein-Rutman Fixed point theorem Principle of Maximum Linear uncoupling Subsystem and supersolution method
86	CaracterizaÃÃo de dois cultivares de Vigna unguiculata em reposta a seca combinada ou nÃo com ozÃnio: regulaÃÃo de proteÃnas membranares (AOX, pUCP E PTOX) / Characterization of two varieties of Vigna unguiculata in response the drought or not combined with ozone: adjustment membrane proteins (AOX, pUCP and PTOX) Francisco Yuri Maia de Sousa 23 November 2012 (has links) Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgico / CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / A FotossÃntese e a respiraÃÃo sÃo bem conhecidos como sendo responsÃveis pela produÃÃo de energia no interior das cÃlulas de plantas. Acoplados Ã estes processos bioenergÃticos, a transferÃncia de elÃtrons que ocorre nas membranas do cloroplasto e mitocÃndria, tambÃm sÃo considerados com tendo potencial de produÃÃo de ROS. PossÃveis interaÃÃes no funcionamento dessas organelas permanecem pouco conhecidas, especialmente em resposta a condiÃÃes de estresse. Sob estas condiÃÃes, sugere-se que as vias de transferÃncia de elÃtrons ligadas Ã AOX, Ã pUCP ou Ã PTOX poderia limitar a formaÃÃo de ROS reduzindo assim danos oxidativos Ãs organelas celulares. Em nosso trabalho, foram selecionadas duas cultivares de Vigna unguiculata, inicialmente caracterizados como sensÃvel (cv 1183) ou tolerante (cv EPACE1) Ã seca. Sob condiÃÃes experimentais, as duas cultivares nÃo apresentaram diferenÃas significativas na sensibilidade Ã seca. Entretanto o cultivar EAPCE1 foi mais tolerante ao ozÃnio com base no desenvolvimento de necrose e de vÃrios parÃmetros fisiolÃgicos (ϕPSII) e bioquÃmicos (teor de glutationa). Para os perfis de expressÃo de genes que codificam AOX, PUCP e PTOX duas respostas foram claramente identificadas no cultivar EPACE1. Em um tempo curto, a expressÃo destas proteÃnas Ã geralmente estimulada. Em um perÃodo mais longo (14 dias), a resposta Ã diferente, dependendo da tipo de estresse. Sob o ozÃnio, um aumento da expressÃo das proteÃnas mitocondriais Ã mantida enquanto o gene que codifica para a PTOX Ã sub-regulada. Sob seca a proteÃna desacopladora do plastÃdio (PTOX) Ã superexpressa. Sob condiÃÃes de combinaÃÃo stress, seca combinada com ozÃnio, o efeito da seca sobre o fluxo de ozÃnio nas folhas nÃo foi alterado, e os genes VuPTOX e VuUCP1b sÃo super-expressos depois de 3 e 7 dias de stress. Este aumento da expressÃo, jÃ observado em resposta Ã seca por aplicada separadamente poderia desempenhar um papel na prevenÃÃo da formaÃÃo de ROS tanto em mitocÃndrias e cloroplastos prevenindo assim os danos causados pelo ozÃnio. / Possible interactions between chloroplast and mitochondria remain poorly known, particularly in response to stress conditions. Under these conditions, it is suggested that the electron transfer pathways linked to AOX or pUCP (mitochondrial uncoupling proteins) and PTOX (plastidial uncoupling protein) could limit the formation of ROS to reduce oxidative damage in cellular organelles. In our work, we selected two cultivars of Vigna unguiculata, cv 1183 and cv EPACE. Under our experimental conditions, both cultivars showed no real differences in sensitivity to drought. However cv EPACE was more tolerant to O3 based on the development of necrosis and several physiological parameters (Fv/Fm, ϕPSII) and biochemical (glutathione content). For the expression profiles of genes encoding AOX, PUCP and PTOX two responses were clearly identified in the cultivar EPACE. On a shortterm, expression of these proteins is generally stimulated. On a longer term (14 days), the answer differs depending on the treatment. Under O3, the strongest expression of mitochondrial proteins is maintained while the gene encoding the PTOX is own-regulated. Under drought only the plastid protein (PTOX) is upregulated. Under conditions of stress combination, drought has little effect on the influx of O3 in the leaves, and the VuPTOX and VuUCP1b genes are up-regulated after 3 and 7 days of stress. This ver-regulation, already observed in response to drought alone could play a role in preventing the formation of ROS in both mitochondria and chloroplasts. Vigna unguiculata seca ozÃnio oxidase alternativa (AOX) oxidase terminal do plastÃdio (PTOX) Vigna unguiculata drought ozone alternative oxidase (AOX) plant uncoupling protein (PUCP) the plastid terminal oxidase (PTOX) BIOQUIMICA
87	Méthodologies de couplage fort des systèmes dynamiques : approches linéaires et non-linéaires Barillon, Franck 29 March 2011 (has links) Dans cette thèse, nous nous sommes intéressés au comportement vibratoire d’un véhicule soumis à une excitation moteur dans deux plages de régimes différentes : basses fréquences (0 – 50 Hz) et moyennes fréquences (200 – 800 Hz). Le but était de fournir des méthodologies numériques permettant de prendre en compte les phénomènes de couplage vibratoires existant entre les différents sous-systèmes constitutifs d’une caisse automobile.En basses fréquences, nous avons adopté une approche globale où chaque sous-système a été caractérisé séparément. Tout d’abord, le comportement de la caisse a été caractérisé expérimentalement et numériquement par une méthode jusqu’alors réservée au domaine aéronautique dite d’appropriation modale. Les résultats numériques ont alors été confrontés aux résultats expérimentaux. Par ailleurs, le comportement non-linéaire en amplitude et en fréquence des pièces de filtration moteur (SMO) a été déterminé sur banc de mesure. Un fort comportement non-linéaire a pu être constaté et ces caractérisations ont été exploitées en construisant des nappes raideur-fréquence-amplitude. Dans un second temps, des méthodes numériques permettant de réaliser l’assemblage non - linéaire de la caisse et du groupe moto – propulseur (GMP) via les pièces de filtration non linéaires ont été mises en place. Pour ce faire, nous avons développé une méthode dite de Balance Harmonique (HBM) qui permet de prédire la dynamique non-linéaire de systèmes complexes. Afin d’appliquer cette méthode à une structure industrielle, nous avons utilisé une méthode de condensation sur les degrés de liberté non-linéaires, technique bien adaptée aux cas de structures linéaires reliées localement par des éléments de liaison non-linéaires. Cette méthode a tout d’abord été validée sur un périmètre restreint comprenant un GMP relié à un bâti rigide par ses pièces de filtration. A cette occasion, des phénomènes non-linéaires importants ont été constatés expérimentalement. Un modèle numérique de GMP a été construit et l’utilisation de la méthode HBM a permis de retrouver ces constats. Enfin, après avoir réalisé l’assemblage non-linéaire des trois sous-systèmes GMP - SMO - Caisse, la structure a été excitée de plusieurs manières différentes : appropriation numérique non-linéaire et excitation réelle d’un GMP. En moyennes fréquences, nous avons présenté dans ce mémoire une étude importante pour le groupe Renault concernant la caractérisation des pièces de filtration en moyennes fréquences. Au cours de la thèse, une méthodologie numérique basée sur la méthode FBS permettant de déconfiner (ou découpler) une suspension moteur initialement reliée à un banc de mesure a été proposée. La faisabilité numérique du déconfinement a ainsi été démontrée. Cette méthode permet donc, en dépit de phénomènes de couplage avec le banc de mesure, d’obtenir le comportement vibratoire intrinsèque de la pièce. / In this thesis we studied the vibratory behaviour of a whole vehicle under engine excitation at low frequencies (0 – 50 Hz) and medium frequencies (200 – 800 Hz). The aim of the thesis was to provide numerical methodologies to take into account coupling effects between all the sub-systems constituting a whole car. In low frequencies, we used a global approach where each subsystem was characterized separately before coupling. First the car body was characterised both experimentally and numerically using a modal appropriation method that is commonly used in the aeronautic field. Numerical shapes of the modes were correlated to experimental shapes. In addition, the amplitude and frequency non linear behaviour of the engine mounts was measured on a test bench. A strong non linear behaviour was observed and stiffness – frequency – amplitude layers were constructed based on those data.Secondly, numerical methods were developed in order to calculate the coupled non linear response between the engine, the engine mounts and the car body. We used a harmonic balance method that allows calculating the non linear dynamics of complex mechanical systems. In order to apply this method to large industrial finite element models, a condensation method on non linear degrees of freedom was developed. This technique is well adapted to problems of linear structures linked together with localnon linear joints. This method was validated on the isolated engine linked to a bench by the engine mounts. Strong non linear phenomena on the rigid body modes of the engine were observed experimentally.A numerical model of the engine was developed and the HBM method allowed reproducing these non linear phenomena. Eventually, the non linear model of the whole vehicle was coupled and excited by different efforts. First we calculated the response of the assembly using the appropriation method. Then, the structure was excited by a real four – cylinder engine excitation.In medium frequencies, we presented an important study for the group Renault concerning the stiffness measurement of the engine mounts. A numerical methodology based on the FRF Based Substructuring(FBS) method was developed. This method was applied to uncouple an engine mount initially coupled to a test bench. The numerical feasibility of the method was proved and allowed to get the own vibratory behaviour of the engine mount despite coupling phenomena with the test bench. Non-linéarités Visco-élastique des liaisons Suspensions moteur Analyse non-linéaire Méthode de Balance Harmonique AFT Méthode d’appropriation modale Couplage Déconfinement Méthode FRF Based Substructuring Vibrations and Structural dynamics Non-linearities Viscoelasticity Engine mounts Nonlinear analysis Harmonic Balance Method AFT Modal Appropriation Method Coupling Uncoupling FRF Based Substructuring Method
88	Multispectral imaging of Sphagnum canopies: measuring the spectral response of three indicator species to a fluctuating water table at Burns Bog Elves, Andrew 02 May 2022 (has links) Northern Canadian peatlands contain vast deposits of carbon. It is with growing urgency that we seek a better understanding of their assimilative capacity. Assimilative capacity and peat accumulation in raised bogs are linked to primary productivity of resident Sphagnum species. Understanding moisture-mediated photosynthesis of Sphagnum spp. is central to understanding peat production rates. The relationship between depth to water table fluctuation and spectral reflectance of Sphagnum moss was investigated using multispectral imaging at a recovering raised bog on the southwest coast of British Columbia, Canada. Burns Bog is a temperate oceanic ombrotrophic bog. Three ecohydrological indicator species of moss were chosen for monitoring: S. capillifolium, S. papillosum, and S. cuspidatum. Three spectral vegetation indices (SVIs) were used to characterize Sphagnum productivity: the normalized difference vegetation index 660, the chlorophyll index, and the photochemical reflectance index. In terms of spectral sensitivity and the appropriateness of SVIs to species and field setting, we found better performance for the normalized difference vegetation index 660 in the discrimination of moisture mediated species-specific reflectance signals. The role that spatiotemporal scale and spectral mixing can have on reflectance signal fidelity was tested. We were specifically interested in the relationship between changes in the local water table and Sphagnum reflectance response, and whether shifting between close spatial scales can affect the statistical strength of this relationship. We found a loss of statistical significance when shifting from the species-specific cm2 scale to the spectrally mixed dm2 scale. This spatiospectral uncoupling of the moisture mediated reflectance signal has implications for the accuracy and reliability of upscaling from plot based measurements. In terms of species-specific moisture mediated reflectance signals, we were able to effectively discriminate between the three indicator species of Sphagnum along the hummock-to-hollow gradient. We were also able to confirm Sphagnum productivity and growth outside of the vascular growing season, establishing clear patterns of reflectance correlated with changes in the local moisture regime. The strongest relationships for moisture mediated Sphagnum productivity were found in the hummock forming species S. capillifolium. Each indicator Sphagnum spp. of peat has distinct functional traits adapted to its preferred position along the ecohydrological gradient. We also discovered moisture mediated and species-specific reflectance phenologies. These phenospectral characteristics of Sphagnum can inform future monitoring work, including the creation of a regionally specific phenospectral library. It’s recommended that further close scale multispectral monitoring be carried out incorporating more species of moss, as well as invasive and upland species of concern. Pervasive vascular reflectance bias in remote sensing products has implications for the reliability of peatland modelling. Avoiding vascular bias, targeted spectral monitoring of Sphagnum indicator species provides a more reliable measure for the modelling of peatland productivity and carbon assimilation estimates. / Graduate peatland peatland restoration peatland monitoring peatland modelling peat peat moss multispectral multispectral imaging multicamera array multitemporal multiple linear regression linear mixed effects Sphagnum sphagna Sphagnum capillifolium Sphagnum papillosum Sphagnum cuspidatum spatiospectral uncoupling near-sensing remote sensing phenospectral phenology phenologies phenospectral bias phenospectral libraries carbon assimilation carbon dioxide carbon dynamics carbon emissions carbon geographies carbon mineralization carbon sequestration carbon source carbon sink carbon storage irrecoverable carbon climate forcing methane Ecological Restoration restoration ecology ecohydrology ecohydrological restoration hydrology ecohydrological gradients ecological integrity reflectance nature based solutions ombrotrophic moss raised peatland mires mires photochemical reflectance index normalized difference vegetation index chlorophyll index climate change climate forcing climate envelope climatope nonvascular vascular bias plant functional type plant functional types structure equation models spectral vegetation indices hummock hollow acrotelm catotelm diplotelmic biocrusts organic soil periodicity moisture deficit senescence rejuvenescence productivity photosynthesis light use efficiency photosynthetic function photosynthetically active radiation water table hydrology rewetting depth to water table landscape change landscape degradation peat subsidence Burns Bog

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