Der Transfer von Chromosom 17 in die menschliche Mammakarzinomzellinie CAL 51 hatte zu einer Reversion des malignen Phänotyps geführt. Aufbauend auf diese Versuche wurde die Genexpression von CAL 51 Zellen versus CAL 17/5 Revertantenzellen mit der "differential display" Methode ana lysiert. Mit dem "differential display" Verfahren konnten 177 differentielle RT-PCR Produkte identifiziert, ausgeschnitten, eluiert und reamplifiziert werden. Ein Vergleich der Sequenzen mit den internationalen Datenbanken ergab: 46% der untersuchten PCR Produkte wiesen keine Homologie zu bekannten Genen auf, 16% zeigten eine hohe Homologie zu EST Sequenzen und cDNA Klonen (unbekannter Gene), 19% eine hohe Homologie zu mitochondrialer DNA und 19% wiesen eine hohe Homologie zu bekannten Genen auf. Die Northern Analyse von 40 ausgewählten Reamplifikaten bestätigte die differentielle Expression in etwa 1/3 der Fälle. Die homologen Gene sind auf unterschiedlichen Chromosomen lokalisiert. Für einige konnte aufgrund ihrer bekannten biologischen Funktionen eine Assoziation mit der Reversion von CAL 51 Zellen vermutet werden. Dazu gehörten u.a. Apolipoprotein J, Sp17 und Profilin. Das Profilingen zeigte eine deutlich erhöhte mRNA- und Proteinexpression in den Revertantenzellen und ist auf Chromosom 17 in der transfizierten Region p13.3 lokalisiert. Profilin ist ein ubiquitäres Protein, das Bindungsstellen für G-Aktin, PIP2 und poly L-Proline besitzt. Die Transfektion von CAL 51 Zellen mit klonierter Profilin cDNA führte bei drei ausgewählten Transfektanten zu einer Suppression des neoplastischen Phänotyps. Die Profilin cDNA Transfektanten zeigten gegenüber den parentalen CAL 51 Zellen und einer Vektortransfektante (ohne Profilin cDNA Insert) ein verlangsamtes Wachstum, eine geringere Koloniebildung in Weichagar, eine differenzierte Strukturbil dung in Matrigel und eine reduzierte Tumorigenität in "nude" Mäusen. Die beobachteten Veränderungen korrelierten zumeist mit der Profilinexpression in den Transfektanten. Eine SSCP- und Sequenzanalyse des Profilingens in CAL 51 Zellen ergab drei Sequenzunterschiede gegenüber der "wildtyp" DNA: Ein homozygoter Basenaustausch in der Promoterregion (A-773G), ein homozygoter Basenaustausch in Exon 3 (C334T) (ohne Aminosäureaustausch) sowie eine heterozygote Deletion in der untranslatierten 3' Region (645delT). Die Bedeutung dieser Sequenzunterschiede ist noch unklar. Mit ersten Northern Analysen von Tumor- und Normalgeweben sowie mehreren Brustkrebszellinien wurde begonnen. Die kleine Anzahl der untersuchten Proben erlaubt noch keine Interpretation der Ergebnisse.

The purpose of this research was to assess the potential impact of climate change on the western Newfoundland tourism industry. Western Newfoundland was chosen as it has a variety of recreational activities that attract tourists. To this end, a mixed methods approach was deemed most appropriate. It allowed for the use of the qualitative procedures of interviews and document analysis as well as the quantitative procedures of statistical climate modeling. The qualitative research demonstrated that there was a desire for further growth in the tourism industry and a general lack of concern for the affects of climate change. The quantitative methods projected that three different recreational and tourism activities studied in this thesis could be altered by climate change. Of the tourism industries examined, snowmobiling was projected to suffer shortened seasons, skiing was projected to see slight losses or to maintain its current season length, and golf was projected to extend its season and increase the number of playable rounds. When the two methods were integrated, there was a gap between the potential changes in the tourism industry and the lack of adaptation plans from the province or the tourism sector. Based on these findings, a series of recommendations were made to the Newfoundland and Labrador Department of Tourism, Culture and Recreation and various tourism operators. This research will contribute a new perspective to the substantial existing literature on tourism, to the growing research on climate change, and to the essential research on Newfoundland and Labrador.

The purpose of this research was to assess the potential impact of climate change on the western Newfoundland tourism industry. Western Newfoundland was chosen as it has a variety of recreational activities that attract tourists. To this end, a mixed methods approach was deemed most appropriate. It allowed for the use of the qualitative procedures of interviews and document analysis as well as the quantitative procedures of statistical climate modeling. The qualitative research demonstrated that there was a desire for further growth in the tourism industry and a general lack of concern for the affects of climate change. The quantitative methods projected that three different recreational and tourism activities studied in this thesis could be altered by climate change. Of the tourism industries examined, snowmobiling was projected to suffer shortened seasons, skiing was projected to see slight losses or to maintain its current season length, and golf was projected to extend its season and increase the number of playable rounds. When the two methods were integrated, there was a gap between the potential changes in the tourism industry and the lack of adaptation plans from the province or the tourism sector. Based on these findings, a series of recommendations were made to the Newfoundland and Labrador Department of Tourism, Culture and Recreation and various tourism operators. This research will contribute a new perspective to the substantial existing literature on tourism, to the growing research on climate change, and to the essential research on Newfoundland and Labrador.

Wolbachia are widely spread, maternally transmitted insect endosymbiotic intracellular bacteria. They have been implicated in the control of several insect transmitted diseases, including dengue, yellow fever, Zika and malaria. Effective pathogen suppression in the insect host is shown to be proportional to the intracellular levels of bacteria. Therefore, understanding the molecular mechanisms underlying Wolbachia accumulation within organisms is extremely important for future epidemic control and research. Using Drosophila as a model insect, our lab has previously observed Wolbachia tropism to stem cell niches. Current work has identified polar cells as an additional site of Wolbachia tropism and demonstrated that Wg/Wnt signaling is important for Wolbachia intracellular accumulation in these somatic cells. In this thesis, we first observed that the Wg/Wnt pathway protein Armadillo also controls Wolbachia levels in the germline cells, indicating the possibility of having a conserved molecular mechanism controlling Wolbachia. Using RNAi and small molecule inhibitors of Shaggy, another component of the canonical Wg/Wnt pathway, we demonstrate that the canonical Wg/Wnt signaling is essential for Wolbachia intracellular accumulation. Our investigation provides fundamental insights into the mechanisms of Wolbachia intracellular accumulation. Furthermore, it offers novel strategies to modulate Wolbachia in non-model insect species, including various disease transmitting Anopheles, Culex, and Aedes. These findings potentially will increase the effectiveness of a Wolbachia-based vector transmitted disease suppression. / 2017-02-28

The thesis describes the complex of basic testing procedures conducted by Euro NCAP company with a view to the examination of pedestrian protection in accordance with EEVC WG 17 and topical instruments used at passenger vehicles for pedestrian protection. It includes a detailed procedure of the creation of an adult head impactor including its certification and the formation of its impact on the bonnet of the vehicle. It is specialized in the studies how material properties influence the size of HIC criterion.

Agriculture is the mainstay of the economy in Malawi and accounts for 40% of the Gross Domestic Product (GDP) and 90% of the export revenues. Corn (maize) is the major cereal crop grown as staple food under rainfed conditions, covers over 92% of the total agricultural area, and contributes 54% of the caloric intake. Corn production is the principle occupation and major source of income for over 85% of the total population in Malawi. Issues of hunger and food insecurity for the entire nation are associated with corn scarcity and low production. Global warming is expected to cause climate change in Malawi, including changes in temperature and precipitation amounts and patterns. These climate changes are expected to affect corn production in Malawi. This study evaluates the impacts of climate change on rainfed corn production in Malawi. Lilongwe District, with about 1,045 square miles of agriculture area, has been selected as a representative area. First, outputs of 15 General Circulation Models (GCMs) under different emission scenarios are statistically downscaled. For this purpose, a weather generator (LARSWG) is calibrated and validated for the study area and daily precipitation as well as minimum and maximum temperature are projected for 15 GCMs for three time horizons of 2020s, 2050s and 2090s. Probability assessment of bounded range with known distributions is used to deal with the uncertainties of GCMs’ outputs. These GCMs outputs are weighted by considering the ability of each model to simulate historical records. AquaCrop, a new model developed by FAO that simulates the crop yield response to water deficit conditions, is employed to assess potential rainfed corn production in the study area with and without climate change. Study results indicate an average temperature increase of 0.52 to 0.94oC, 1.26 to 2.20oC and 1.78 to 3.58oC in the nearterm (2020s), mid-term (2050s) and long-term (2090s) future, respectively. The expected changes in precipitation during these periods are -17 to 11%, -26 to 0%, and -29 to -3%. Corn iii yields are expected to change by -8.11 to 0.53%, -7.25 to -14.33%, and -13.19 to -31.86%, during the same time periods. The study concludes with suggestion of some adaptation strategies that the Government of Malawi could consider to improve national food security under climate change.

As a result of a growing number of Member States of the European Union the idea of unification and co-ordination of legal changes,including tax systems of each country, is growing stronger. The common consolidated corporate tax base (CCCTB) should establish unified rules for determining the tax base, and consequently this would eliminate double taxation, cross-border income, or tax evasion and suppression of tax competition among the states of the European Union. This work deals with the redistributive mechanism of the CCCTB, which would alocate the CCCTB to the group of the individual companies. Interim result of the discussion is to create a distribution threefactors formula consisting of variables - work, asset and sales. I applied the current conclusions of the European Commission to the Czech company Skoda Auto,a.s., whose parent company is in Germany.

Die physische Nähe zwischen Genpromotoren und regulatorischen Elementen (Enhancer) spielt eine entscheidene Rolle in der Genexpression, um präzise räumliche und zeitliche Genexpressionmuster während der Embryogenese zu erzeugen. Abhängig von der Aktivität der Zielgene lassen sich zwei Typen von Interaktionen unterscheiden. Zum einen führen dynamische Enhancer-Promoter Interaktionen unmittelbar zur Genexpression, wohingegen in anderen Fällen stabile Interaktionen bereits vor der Genexpression existieren. In der vorliegenden Studie wurde die Rolle der stabilen Interaktion zwischen dem Shh Gen und dem Extremitätenenhancer, der ZRS, während der Embryonalentwicklung in der Maus untersucht. Der Verlust der konstitutiven Transkription, die den ZRS Enhancer abdeckt, führte zu einer Verschiebung innerhalb der Shh-ZRS Kontakte und einer moderaten Reduzierung der Shh Genexpression. Im Gegensatz dazu führte die Mutation von CTCF Bindungsstellen, die den ZRS Enhancer umgeben, zu einem Verlust der stabilen Shh-ZRS Interaktion und einem 50%igen Rückgang in der Shh Genexpression. Dieser Expressionsverlust hatte jedoch keine phänotypischen Auswirkungen in den Deletionsmutanten, was darauf hindeutet, dass die restliche Genaktivität und Enhancer-Promotor-Interaktion über einen zusätzlichen, CTCF-unabhängigen Mechanismus erfolgt. Erst die kombinierte Deletion von CTCF-Bindungsmotiven und einem hypomorphen ZRS-Allel führte zu einem fast vollständigen Expressionsverlust von Shh und damit zu einem schweren Funktionsverlust und Gliedmaßen-Agenesie. Die hier präsentierten Ergebnisse zeigen, dass die stabile Chromatinstruktur am Shh Locus von mehreren Komponenten getragen wird und die physicalische Interaktion zwischen Enhancern und Promotern für eine robuste Transkription während der Embryonalentwicklung benötigt werden. / Long-range gene regulation involves physical proximity between enhancers and promoters to generate precise patterns of gene expression in space and time. However, in some cases proximity coincides with gene activation, whereas in others preformed topologies already exist before activation. In this study, we investigate the preformed configuration underlying the regulation of the Shh gene by its unique limb enhancer, the ZRS, in vivo during mouse development. Abrogating the constitutive transcription covering the ZRS region led to a shift within the Shh-ZRS contacts and a moderate reduction in Shh transcription. Deletion of the CTCF binding sites around the ZRS resulted in a loss of the Shh-ZRS preformed interaction and a 50% decrease in Shh expression but no phenotype, suggesting an additional, CTCF-independent mechanism of promoter-enhancer communication. This residual activity, however, was diminished by combining the loss of CTCF binding with a hypomorphic ZRS allele resulting in severe Shh loss-of-function and digit agenesis. Our results indicate that the preformed chromatin structure of the Shh locus is sustained by multiple components and acts to reinforce enhancer-promoter communication for robust transcription.

Naturstoffe sind eine der wichtigsten Quellen für die Entwicklung neuer Pharmazeutika. Eine Vielzahl von bioaktiven Substanzen mit potentieller Anti-Krebs, Anti-HIV oder antimikrobieller Wirkung wurde aus der Gruppe der Cyanobakterien isoliert. Die meisten dieser Metabolite sind Peptide oder besitzen peptid-ähnliche Strukturen und werden nicht-ribosomal von großen, modular aufgebauten Enzymkomplexen gebildet. Vor kurzem konnte anhand der Patellamide gezeigt werden, dass zyklische Peptide auch ribosomal hergestellt werden können. Microcystis aeruginosa NIES298 produziert eine Reihe von Sekundärmetaboliten, unter anderem die nicht-ribosomalen Peptide Microcystin und Aeruginosin. Zwei weiteren von diesem Stamm produzierten Peptiden, Microcyclamid und Microviridin B, konnten bislang noch keine Gene zugeordnet werden. In dieser Studie wurden ribosomale Biosynthesewege für beide Peptidfamilien identifiziert. Die zur Biosynthese des cytotoxischen Hexapeptids Microcyclamid notwendigen Enzyme zeigen eine hohe Ähnlichkeit zu den Patellamid-Enzymen und weisen auf ähnliche Biosynthesemechanismen hin. Ein völlig neuer Syntheseweg, in dem bis dahin unbekannte ATP-grasp-Ligasen eine Rolle spielen, konnte für den trizyklischen Proteaseinhibitor Microviridin gefunden werden. Die erfolgreiche heterologe Expression dieses Peptids in E. coli bietet die Möglichkeit Bibliotheken von Microviridin-Varianten mit neuen oder verbesserten Bioaktivitäten zu konstruieren. Die systematische Suche nach ähnlichen Biosynthesegenen in Microcystis Laborstämmen und Gewässerproben zeigte eine weite Verbreitung und eine große Diversität der untersuchten Peptidklassen in Cyanobakterien, und stellt die Frage nach der natürlichen Funktion dieser Metabolite. Um erste Hinweise zu erhalten, wurden Trankriptions- und Expressionsstudien der Biosynthesegene durchgeführt. Schließlich konnten, mit Hilfe des so genannten „genome-mining“, neue Varianten der untersuchten Peptidklassen gefunden und aufgeklärt werden. / Microbial natural products represent a major source for the development of new therapeutic agents. A diverse array of compounds is produced by cyanobacteria, a heterogenous group of aerobic photoautotrophs. A variety of bioactive metabolites with potential anti-cancer, anti-microbial and anti-HIV activities have been isolated. Most of the compounds are peptides or possess peptidic structures and are usually made by large nonribosomal assembly lines. However, a ribosomal origin has recently been demonstrated for the biosynthesis of patellamides, cytotoxic cyclic peptides produced by cyanobacterial symbionts of ascidians. Microcystis aeruginosa NIES298 produces various peptides including microcystin, aeruginosin, microviridin and microcyclamide. For the latter two classes of peptides ribosomal biosynthesis pathways could be identified in the course of this study. The cytotoxic hexapeptide microcyclamide is formed through the activity of a set of enzymes closely related to those involved in patellamide biosynthesis. The multicyclic microviridin family of protease inhibitors are synthesised from a precursor peptide by a unique pathway involving uncharted ATP-grasp type ligases as well as an N-acetyltransferase and a specialised transporter peptidase. The successful expression of microviridin B in E. coli provides a promising base for engineering novel variants. Screening of Microcystis laboratory strains and field samples revealed a wide-spread occurrence and a great natural variety for both peptide classes, raising the question of the ecological role of such small cyclic peptides. Attempting to obtain some first hints to answer that question, transcription and expression studies of biosynthetic genes were performed. Finally, this work showed that such scanning approaches could lead to the discovery of novel peptide variants and demonstrated new examples of succesful genome mining.

Der Transkriptionsfaktor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) koordiniert Proliferationshemmung und Differenzierung von myeloiden VorlŠuferzellen und Adipozyten. C/EBPa ist ein transkriptioneller Aktivator von abstammungspezifischen Genen und blockiert den Zellzyklus durch Repression von proliferationsfšrdernden E2F Zielgenen. Die hier gezeigten Daten zeigen, dass auch umgekehrt E2F die transkriptionelle und differenzierungsfšrdernde AktivitŠt von C/EBPa entgegenwirkt. Somit besitzen E2F-C/EBPa eine zentrale Schalterfunktion zwischen Proliferation und Differenzierung. Der Repressionsmechanismus durch E2F ist in mehreren Aspekten neuartig: Zum erstenmal wurde gezeigt, dass E2F einen anderen Transkriptionsfaktor reprimieren kann. E2F reprimiert die transkriptionelle AktivitŠt von C/EBPa ohne Bindung an cis-regulatorischen Elemente, sondern durch direkte Protein-Protein Interaktionen, die die Bindung von C/EBPa an DNA verhindern. Diese Form der transkriptionellen Repression geschieht unabhŠngig von "Pocket-Proteinen''". Patienten mit Akuter Myeloiden LeukŠmie (AML) weisen hŠufig eine gestšrte DNA Bindung von C/EBPa auf, welche ursachlich fŸr granulozitŠren Funktionsstšrungen sein kšnnte. Daher wŠre es wichtig zu analysieren ob E2F die DNA Bindung von C/EBPa in AML Patienten beeintrŠchtigt und ob auf E2F gerichtete Therapien granulozitŠre Reifung wiederherstellen. C/EBPa blockiert Zellproliferation durch vielseitigen Mechanismen. Hier wurde gezeigt, dass C/EBPa mit UBF1, dem Co-Aktivator der RNA Polymerase I, an chromosomalen Foci positioniert wird. Eine €hnlichkeit zu anderen fokalen Strukturen suggeriert, dass C/EBPa die Transkription von Polymerase I regulierten rRNA Gene reprimieren und somit ribosomale Biogenese beeintrŠchtigen kšnnte. Die Assoziation zwischen C/EBPa und UBF1 wird durch die Histon-Methyltransferase SUV39H1 stimuliert. Demnach kšnnte die antiproliferative Funktion von C/EBPa nicht nur auf der Regulierung von RNA Pol II-abhŠngiger Transkription, sondern auch auf der Repression von RNA Pol I regulierter rRNA Synthese basieren. / The transcription factor CCAAT/Enhancer-Binding Protein alpha (C/EBPa) coordinates proliferation arrest and differentiation of myeloid progenitors and adipocytes. C/EBPa acts as a transcriptional activator of lineage specific genes and blocks the cell cycle by repressing transcription of E2F-regulated genes. Data presented here suggest that also inversely E2F interferes with the transcriptional activity of C/EBPa, counteracting C/EBPa-mediated differentiation processes. Thus, E2F-C/EBPa are part of a switch mechanism between proliferation and differentiation. The mechanism by which E2F suppresses C/EBPa-mediated transactivation is novel in several aspects. E2F acts as a co-repressor of another transcription factor, C/EBPa, without binding to cis-regulatory elements, but by direct protein-protein interactions that abolish the binding of C/EBPa to DNA. This mechanism of transcriptional repression occurs independent of pocket proteins. Disturbed DNA binding of C/EBPa is often observed in AML patients suggesting a causative role in granulocytic disorders. Thus, it would be of main interest to analyze whether E2F mediates disruption of C/EBPa''s DNA-binding in AML patients and whether therapies directed against E2F could restore granulocytic maturation. Despite the extensive knowledge of mechanisms involved in the inhibitory function of C/EBPa, it has not been addressed whether C/EBPa may impinge on cell proliferation by affecting the ribosomal biogenesis of a cell. This work demonstrates an association of C/EBPa to the RNA Pol I transcription factor UBF1, both proteins retained in large chromosomal foci. Similarities to other focal structures associated to UBF1, suggest that C/EBPa may repress transcription of Pol I-transcribed rRNA genes, and thus affect ribosomal biogenesis. The enrichment of C/EBPa at sites of UBF1 is induced by the histone methyltransferase SUV39H1. Thus, C/EBPa may not only control lineage commitment and cell proliferation by regulating genes transcribed by RNA Pol II, but also may act as a repressor of RNA Pol I mediated rRNA synthesis.