O objetivo desta pesquisa consiste em investigar o significado da avaliação em matemática na perspectiva histórico-cultural, focalizando a teoria da atividade. Para o desenvolvimento dessa investigação, foi formado o grupo colaborativo da Oficina Pedagógica de Matemática de Ribeirão Preto (OPM/RP), constituído por professoras da Educação Infantil e séries iniciais do Ensino Fundamental, as quais foram sujeitos da pesquisa. A função principal do grupo colaborativo foi proporcionar uma formação orientada às professoras sobre o ensino de matemática na perspectiva histórico-cultural, com o intuito de levantar dados sobre o processo de apropriação dos conhecimentos matemáticos pelas docentes. O processo formativo, pautado nos pressupostos da Atividade Orientadora de Ensino, privilegiou a apresentação de situações-problema, cujas soluções possibilitaram a elaboração de conhecimentos, referentes não apenas aos conteúdos matemáticos, mas, também, à prática docente: ensinar, aprender e avaliar em matemática. Na análise dos dados, utilizamos o conceito de isolado (CARAÇA, 1989, MOURA, 2000) como princípio teórico-metodológico para a compreensão do fenômeno em desenvolvimento. Os dados foram organizados em três isolados aprendizagem docente, organização do ensino e prática pedagógica, tendo em vista a relação de interdependência e fluência entre os mesmos. Na relação entre a aprendizagem docente (apropriação dos conceitos teórico-metodológicos sobre o ensino de matemática), a organização do ensino (como elaborar atividade de ensino para que os alunos se apropriem dos conceitos) e a prática pedagógica (como colocar os conceitos teórico-metodológicos em ação na sala de aula) é que o professor se desenvolve profissionalmente, isto é, aprende a ser professor em um contínuo processo formativo. A síntese sobre o processo de aprendizagem docente foi importante para a sistematização dos elementos norteadores da avaliação em matemática na perspectiva da teoria histórico-cultural. Os resultados evidenciaram que a avaliação constitui-se em um constante processo de análise e síntese e seu direcionamento é dado pelo objetivo da atividade de ensino elaborada pelo professor, ou seja, sua intencionalidade pedagógica. A característica principal da avaliação consiste no acompanhamento do processo de ensino e aprendizagem, por meio da análise do sistema de atividade, na dinâmica entre a atividade de ensino e a atividade de aprendizagem, considerando os elementos constitutivos da atividade (necessidade, motivos, objetivos, ações e operações). Por meio da análise das ações de ensino e de aprendizagem, tendo como parâmetros as características do pensamento teórico (reflexão, análise e planificação teórica), concluímos que a avaliação é mediadora entre a atividade de ensino elaborada pelo professor e a atividade de aprendizagem realizada pelos escolares. / This research aims to investigate the meaning of evaluation in mathematics from the historical-cultural perspective, focusing on the activity theory. In order to develop the investigation, a collaborative group was formed from the Oficina Pedagógica de Matemática de Ribeirão Preto São Paulo (Math Pedagogic Workshop of Ribeirão Preto OPM/RP), constituted by teachers of Educação Infantil (Brazilian Primary Education) and teachers of the first years of Ensino Fundamental (Brazilian Secondary Education), which were participants of this research. The main role of the collaborative group was to offer an oriented development to the teachers about the teaching of mathematics from the historical-cultural perspective, aiming at collecting data on the process of appropriation of mathematical knowledge by the teachers. The development process, based on the premises of the Teaching Oriented Activity, privileged the introduction of problem-solving situations, whose solutions allowed the elaboration of knowledge, referring not only to math contents, but also to the teaching practice: teaching, learning and evaluating in mathematics. In analyzing data, we used the concept of isolated (CARAÇA, 1989, MOURA, 2000) as a theoreticalmethodological principle to understand the phenomenon under development. Data were organized into three isolated teachers learning, teaching organization and pedagogical practice, having in mind the relationship of interdependency and fluency among them. It is in the relationship among the teachers learning (appropriation of theoretical-methodological concepts on the teaching of mathematics), the organization of teaching (how to elaborate the teaching activity so that students can appropriate concepts) and the pedagogical practice (how to put theoretical-methodological concepts into practice in the classroom) that the teacher develops professionally, that is, learns to become a teacher through a continuous developing process. The synthesis about the teachers learning process was important to systematize the guiding elements of evaluation in mathematics from the historical-cultural perspective. Results showed that evaluation is constituted of a continuous process of analysis and synthesis, and its north is set by the aim of the teaching activity elaborated by the teacher, that is, his/her pedagogical intentionality. The main characteristic of evaluation lies on keeping track of the teaching and learning process, by means of the analysis of the activity system, along the dynamics between the teaching activity and the learning activity, taking into consideration the activity constitutive elements (need, reasons, objectives, actions and operations). By analyzing the actions of teaching and the actions of learning, having as parameters the characteristics of the theoretical thought (reflection, analysis and theoretical rationalization), we conclude that evaluation is a mediator between the teaching activity elaborated by the teacher and the learning activity performed by the school students.

ABSTRACT When depressive illness has its onset during adolescence it can exert a profound negative influence upon the individual and their daily functioning. Early identification of young people at risk of, or already experiencing, depressive symptoms is critical to enabling targeted intervention from clinicians, including occupational therapists. Early identification, however, is hampered by an incomplete understanding of how the disorder manifests in the daily lives of adolescents. The mental health determinants and sequelae of engagement in everyday activities represent core concerns of occupational therapists. As part of therapy with adolescents in Child and Youth Mental Health Services (CYMHS), occupational therapists endeavour to improve health by assessing time use, and engaging in interventions which explicitly target involvement in daily activity. Notwithstanding current practice, evidence for a link between actual time use and depressive symptom severity is scant. Background and Research Aims In this thesis the time use of adolescents is explored and related to depressive symptom severity in a series of three cross sectional studies. In keeping with ecologically- and occupation-focussed theoretical models of health and adolescent development (Bronfenbrenner & Morris, 2006; Poulsen & Ziviani, 2004; World Health Organization, 2002), a novel approach to the study of time use has been taken. The studies quantify the amount of time that adolescents are exposed to the various conditions inherent in activities and their ecological settings. In conceptualising time use as ‘exposure’, attention is drawn specifically to common affordances of activities. In this thesis these are described as ‘underlying dimensions’ of time use. Key underlying dimensions of interest include (a) ‘physical exertion’ in daily time use, (b) time spent with different social partners, and (c) time engaged in structured activities. The studies make use of cumulative estimates of time use by summing each episode of exposure to the underlying dimensions of time use, across the out-of-school hours. This research was conducted with the aims of (a) aiding early identification of adolescents who are vulnerable to depressive illness, (b) examining the evidence for the assessment of time use in CYMHS, (c) identifying aspects of adolescent time use which are appropriate targets for occupational therapists’ intervention, and (d) directing future research into the effectiveness of time use-focussed interventions offered by occupational therapists to adolescents who are vulnerable to depressive illness. Methodology and Study Findings All the studies involve secondary analysis of interview and time diary data collected from a nationally representative sample of approximately 730 adolescents (13-18 years) participating in the Panel Study of Income Dynamics Child Development Supplement II. Data were gathered in 2002/2003 by the Institute of Social Research at the University of Michigan in America. Studies 1 and 2 employ ordinal logistic regression analyses to separately explore two of the underlying dimensions of time use, namely the physical exertion in daily activity, and the time spent engaged in activity alone or with key social partners (including parents, siblings and friends). These studies indicate that time devoted to physical activity of moderate to vigorous intensity does not predict depressive symptom severity, breadth of participation in sporting extracurricular activity may be protective against depressive illness for males. For both genders, factors related to the positive or negative aspects of social relationships (such as adolescents’ ratings of how close they felt to parents, and the extent of risky behaviour among friends) appear to be stronger predictors of depressive symptomatology than the actual amount of time engaged in activity with others. Study 3 is informed conceptually and methodologically by the findings of Studies 1 and 2, and uses structural equation modeling, to examine a complex multivariate model of time use and depressive symptoms. This model incorporates a large range of factors which are theorized to influence the qualitative experience of spending time in a given context (e.g., adolescents’ perceptions of friend acceptance, which may influence the psychological impact of a prolonged encounter with friends). Study 3 reveals that the quantity of time over the course of a weekday or weekend day that adolescents are exposed to different types of activity (e.g., structured extracurricular activity, shared activity with parents) has no direct association with depressive symptom severity. However, an indirect path is identified, whereby the amount of time devoted to joint activity with parents is linked with depressive symptom severity via adolescent perceptions of parent acceptance. Conclusions and Recommendations The studies of this thesis have shown that the conceptualisation and quantification of time use as cumulative exposure to some specific underlying dimensions of engagement, are of little direct assistance in identifying young people who are vulnerable to depressive illness, however, this information may inform a range of clinical practices for occupational therapists working in CYMHS. Information on time use is therefore worth gathering in such services, particularly when it provides insights into social contact. It has been clearly and consistently identified that time use and its links with depression cannot be fully appreciated without considering the nature of adolescents’ relationships with their social partners in time use. Findings indicate that when time spent engaged in joint activity with parents is scant, or when poor relationships with parents are reported, adolescents at risk of depression are likely to benefit from therapy which is directed towards enhancing the quantity and quality of parent-adolescent interactions. The significance of this research lies in the generation of evidence to support further research into time use and its implications for mental health. Greater support for the specialist role of occupational therapists in addressing time use concerns will be gained through future studies which explore the immediate affective experience of spending time in activity, the personal and environmental contextual factors which mediate these experiences, and the implications of such experiences for mental health. Further to the insights gained regarding time use and depressive illness, the thesis presents an approach to research (i.e., a secondary analysis) and research methodologies which may be used by occupational therapists to answer complex questions regarding occupational engagement across the lifespan. Keywords: Adolescence, Depression, Internalizing Disorder, Time Use, Time Diary, Physical Activity, Social Contexts, Structured Extracurricular Activity, Organised Activity, Structural Equation Modeling. Australian and New Zealand Standard Research Classifications (ANZSRC): 111714 Mental Health 100%.

The purposes of this study were to test the effect of four-week PACE&copy / program in increasing participants&rsquo / physical activity level and stages of change and examine the relationship between physical activity level and stages of change. A controlled trial, experimental design was applied. Physical activity level and stages of change were recorded at baseline and at study conclusion. For the data collection What is Your PACE Score Questionnaire, FADA Questionnaire and Physical Activity Readiness Questionnaire (PAR&ndash / Q) were used. Descriptive statistics, one way repeated measures of ANOVA and simple linear correlation were used to analyze data. Participants were 182 women Middle East Technical University personnel from different units. Study participants were randomized into intervention and control groups. 87 women received the PACE&copy / program were compared 95 women in the control group who did not receive PACE&copy / program. The intervention group received PACE&copy / program with respect to their stages and a booster phone call or e-mail two weeks later after counseling. After 4 weeks, all participants were reassessed for stages of change and physical activity level. There was a significant difference in measurement of physical activity level and stages of change between intervention and control group before and after intervention. The intervention group&rsquo / physical activity level (p &lt / .05) and stages of change (p &lt / .10) increased but the control group decreased. Besides, changes in physical activity level were moderately and positively correlated with stages of change.

The health and well-being of individuals is related to their activity-travel patterns. Individuals who undertake physically active episodes such as walking and bicycling are likely to have improved health outcomes compared to individuals with sedentary auto-centric lifestyles. Activity-based travel demand models are able to predict activity-travel patterns of individuals at a high degree of fidelity, thus providing rich information for transportation and public health professionals to infer health outcomes that may be experienced by individuals in various geographic and demographic market segments. However, models of activity-travel demand do not account for the attitudinal factors and lifestyle preferences that affect activity-travel and mode use patterns. Such attitude and preference variables are virtually never collected explicitly in travel surveys, rendering it difficult to include them in model specifications. This paper applies Bhat’s (2014) Generalized Heterogeneous Data Model (GHDM) approach, whereby latent constructs representing the degree to which individuals are health conscious and inclined to pursue physical activities may be modeled as a function of observed socio-economic and demographic variables and then included as explanatory factors in models of activity-travel outcomes and walk and bicycle use. The model system is estimated on the 2005-2006 National Health and Nutrition Examination Survey (NHANES) sample, demonstrating the efficacy of the approach and the importance of including such latent constructs in model specifications that purport to forecast activity and time use patterns. / text

The overall goal of the dissertation is to contribute to the growing literature on the activity-based framework by focusing on the modeling of choices that are influenced by land-use and travel environment attributes. An accurate characterization of activity-travel patterns requires explicit consideration of the land-use and travel environment (referred to as travel environment from here on). There are two important categories of travel environment influences: direct (or causal) and indirect (or self-selection) effects. The direct effect of travel environment refers to how travel environment attributes causally influence travel choices. This direct effect may be captured by including travel environment variables as exogenous variables in travel models. Of course, determining if a travel environment variable has a direct effect on an activity/travel choice of interest is anything but straightforward. This is because of a potential indirect effect of the influence of the travel environment, which is not related to a causal effect. That is, the very travel environment attributes experienced by a decision maker (individual or household) is a function of a suite of a priori travel related choices made by the decision maker. The specific emphasis of the current dissertation is on moving away from considering travel environment choices as purely exogenous determinants of activity-travel models, and instead explicitly modeling travel environment decisions jointly along with activity-travel decisions in an integrated framework. Towards this end, the current dissertation formulates econometric models to analyze multidimensional choices. The multidimensional choice situations examined (and the corresponding model developed) in the research effort include: (1) reason for residential relocation and associated duration of stay (joint multinomial logit model and a grouped logit model), (2) household residential location and daily vehicle miles travelled (Copula based joint binary logit and log-linear regression model), (3) household residential location, vehicle type and usage choices (copula based Generalized Extreme Value and log-linear regression model) and (4) activity type, travel mode, time period of day, activity duration and activity location (joint multiple discrete continuous extreme value (MDCEV) model and multinomial logit model (MNL) with sampling of alternatives). The models developed in the current dissertation are estimated using actual field data from Zurich and San Francisco. A variety of policy exercises are conducted to illustrate the advantages of the econometric models developed. The results from these exercises clearly underline the importance of incorporating the direct and indirect effects of travel environment on these choice scenarios. / text

Nos últimos anos, os metabólitos isolados de fungos marinhos vêm ganhando considerável atenção em razão de possuírem estruturas químicas únicas com diversas atividades biológicas já descritas, incentivando novas pesquisas na área. A tricomoníase é a doença sexualmente transmissível (DST) de origem não viral mais comum no mundo, estando associada a sérias consequências à saúde, sendo relatado um aumento no número de isolados clínicos resistentes ao tratamento de escolha. Infecções causadas por bactérias com diferentes mecanismos de resistência, representam um grande desafio para a saúde pública atual, acarretando em altas taxas de mortalidade e morbidade. As bactérias patogênicas dispõem de fatores de virulência, como a formação de biofilme, que agravam infecções tornando-as persistentes. Devido ao potencial biológico dos produtos de origem marinha e a importância dessas infecções, o objetivo deste estudo foi avaliar a atividade anti-T. vaginalis, antimicrobiana e antibiofilme de moléculas obtidas da fermentação de fungos associados a organismos marinhos. As 14 cepas fúngicas foram isoladas de esponjas e corais marinhos, obtidos da costa do estado de Alagoas, Brasil. Após produção do metabólito, o micélio foi separado do meio. O micélio foi extraído com metanol e o meio com acetato de etila. As frações foram submetidas aos ensaios de atividade anti-T.vaginalis, antimicrobiana e antibiofilme (inibição da formação e erradicação). As frações que demonstraram atividade foram submetidas ao ensaio de hemólise, avaliação da citotoxicidade (HMVII e Vero) e toxicidade em modelo de Galleria mellonella. A fração que demonstrou os melhores resultados nos ensaios de atividade foi submetida ao fracionamento bioguiado. As frações orgânicas dos fungos Aspergillus niger (FMPV 03) e complexo Trichoderma harzianum/Hypocrea lixii (FMPV 09) foram ativas frente ao T. vaginalis ATCC 30236, com valores de MIC de 2 mg/mL e 1 mg/mL, respectivamente. Quando investigadas, essas frações mantiveram a atividade frente ao isolado clínico resistente ao metronidazol (TV-LACM2R), apresentado os mesmo valores de MIC encontrados para o isolado ATCC. Para a atividade antimicrobiana, as frações orgânicas do Aspergillus niger (FMPV 03), Aspergillus tubingensis (FMPV 06), complexo Trichoderma harzianu/Hypocrea lixii (FMPV 09) e Aspergillus sydowii (FMPV 10) foram ativas contra S. epidermidis ATCC 35984. Ainda, frente a P. aeruginosa ATCC 27853 somente as frações orgânicas do Aspergillus niger (FMPV 03) e Aspergillus tubingensis (FMPV 06) demonstraram atividade. Neste ensaio, para ambas as bactérias, os valores de MIC não ultrapassaram 1,5 mg/mL. A atividade destas frações também foi observada frente às mesmas bactérias no ensaio de antiformação de biofilme, já que ocorreu a morte das células. A habilidade de erradicar biofilmes foi detectada somente para a fração orgânica do fungo Aspergillus flavus (FMPV 01), o qual foi capaz de remover 52% do biofilme já formado de S. epidermidis ATCC 35984. A ausência de hemólise dos eritrócitos foi observada em todas as frações ativas estudadas. Na avaliação da citotoxicidade in vitro frente às linhagens celulares HMVII e Vero, apenas a fração orgânica do Aspergillus niger (FMPV 03), não apresentou efeito citotóxico. No entanto, no ensaio de avaliação da toxicidade in vivo, nenhuma das amostras testadas causou redução na sobrevivência das larvas de Galleria mellonella. Então, a fração orgânica do fungo Aspergillus niger (FMPV 03) foi submetida ao fracionamento bioguiado utilizando coluna RP-18. Sete frações foram obtidas, sendo que a primeira (100% água), foi ativa contra T. vaginalis, S. epidermidis e P. aeruginosa. Quando submetida à Cromatografia em Camada Delgada (CCD), demonstrou quatro bandas que foram coradas com anisaldeído-ácido sulfúrico e ninhidrina. Além disso, a fração 100% água não demonstrou redução da sobrevivência das larvas de Galleria mellonella, nas três concentrações testadas. Portanto, a gama de atividades relatadas corrobora o potencial dos fungos marinhos na produção de moléculas bioativas. / In recent years, the isolated metabolites from marine fungi have been attracted considerable attention due to unique chemical structures with diverse biological activities, encouraging further research in the area. Trichomoniasis is the most prevalent non-viral sexually transmitted disease (STD) worldwide. This has been linked to serious health consequences and an increase in the number of clinical isolates resistant to the treatment of choice has been reported. Infections caused by bacteria with different resistance mechanisms represent a major challenge to the current public health, resulting in high rates of mortality and morbidity. Pathogenic bacteria present virulence factors, such as biofilm formation, which migth enhance the persistence of infections. Due to the biological potential of marine products and the importance of these infections, the aim of this study was to evaluate the anti-T. vaginalis, antimicrobial and antibiofilm activities of the obtained molecule from the fermentation of fungi associated with marine organism. The 14 fungal strains were isolated from sponges and corals marine obtained from the coast of Alagoas, Brazil. After production of the metabolite the mycelium was separated from the medium. The mycelium was extracted with methanol and the medium with ethyl acetate. The fractions were subjected to the assays anti-T. vaginalis, antimicrobial and antibiofilm (inhibition of the formation and eradication) activities. The fractions that showed activity were subjected to the assays of hemolysis, cytotoxicity evaluation (HMVII and Vero) and toxicity Galleria mellonella model. The fraction which showed the best results in activity assays was subjected to fractionation bioguided. The organic fraction of Aspergillus niger (FMPV 03) and Trichoderma harzianum/Hypocrea lixii complex (FMPV 09) were active against T. vaginalis ATCC 30236 with MIC values of 2 mg/mL and 1 mg/mL, respectively. When investigated, these fractions maintained activity against resistant clinical isolate to metronidazole (TV-LACM2R), presented the same MIC values found to isolate ATCC. For the antimicrobial activity, the organic fractions of Aspergillus niger (FMPV 03), Aspergillus tubingensis (FMPV 06), Trichoderma harzianum/Hypocrea lixii complex (FMPV 09) and Aspergillus sydowii (FMPV 10) were active against S. epidermidis ATCC 35984. Even, against P. aeruginosa ATCC 27853 only the organic fractions of Aspergillus niger (FMPV 03) and Aspergillus tubingensis (FMPV 06) demonstrated activity. In this test, for both bacteria, MIC values did not exceed 1.5 mg/mL. The activity of these fractions was also observed across the same bacteria in the antibiofilm formation assay, since cell death occurred. The ability to eradicate biofilms was detected only for the organic fraction of the fungus Aspergillus flavus (FMPV 01) which was able to remove 52% of the already formed biofilm of S. epidermidis ATCC 35984. The absence of hemolysis of red cells was observed in all active fractions studied. In the assessment of cytotoxicity in vitro against the cell lines HMVII and Vero, only the organic fraction of Aspergillus niger (FMPV 03), showed no cytotoxic effect. However, in the test evaluation of in vivo toxicity, none of the samples tested caused a reduction in the survival of the larvae of Galleria mellonella. Then, the organic fraction of the fungus Aspergillus niger (FMPV 03) was submitted to bioguided fractionation using RP-18 column. Seven fractions were obtained, of which the first (100% water), was active against T. vaginalis, S. epidermidis e P. aruginosa. When subjected to thin layer chromatography (TLC) showed four bands were stained with ninhydrin and anisaldehyde-sulfuric acid. In addition, 100% water fraction showed no reduction of the survival of larvae of Galleria mellonella at the three concentrations tested. Therefore, the range of activities reported corroborates the potential of marine fungi to produce bioactive molecules.

Cumarinas são estruturas promissoras e diversas atividades biológicas têm sido atribuídas a esses metabólitos secundários vegetais. Estudos sugerem que o mecanismo antifúngico desses compostos esteja correlacionado com a atividade antioxidante. A reação da tirosinase, que produz radicais livres, está envolvida no processo de melanização do fungo Sporothrix schenckii, o causador de micose subcutânea de maior incidência no sul do Brasil. A inibição dessa enzima foi recentemente reportada para extrato de Pterocaulon (Asteraceae), planta rica em cumarinas e usada na medicina tradicional do Brasil para tratamento tópico de micoses e na medicina popular da Argentina como anticâncer. O objetivo desse trabalho foi investigar in vitro a atividade antifúngica, correlacionando-a com atividade antioxidante, de extratos de espécies de Pterocaulon e de 6,7-metilenodioxicumarinas isoladas e também de 4-metilcumarinas obtidas por síntese, bem como a investigação da atividade citotóxica de algumas destas moléculas. As 6,7-metilenodioxicumarinas foram isoladas de P. balansae e P. lorentzii, enquanto que as 4-metilcumarinas foram sintetizadas via Pechmann por micro-ondas. A atividade antifúngica contra Sporotrix schenckii foi realizada conforme manual do Clinical and Laboratory Standards Institute. O estudo das propriedades eletroquímicas foi obtido por voltametria cíclica e a capacidade antioxidante pelo método espectofotométrico DPPH (1,1-difenil-2-picrilhidrazil) e pelo método fluorimétrico ACAP contra radicais peroxil. A análise dessa atividade mostrou que os extratos metanólicos de espécies de Pterocaulon (P. polystachyum, P. balansae, P. lorentzii, P. lanatum e P. cordobense) foram ativos frente às cepas do fungo S. schenckii, sendo o extrato de P. polystachyum o mais ativo, apresentando Concentração Inibitória Mínima (CIM) compreendida entre 156 e 312 μg/mL. O fracionamento dos extratos lipofílicos de P. balansae e P. lorentzii levou ao isolamento de três metilenodioxicumarinas. Dados cristalográficos da 5-metóxi-6,7-metilenodioxicumarina, inéditos, foram depositados no Cambridge Crystallographic Data Centre 779123. As cumarinas sintéticas foram obtidas em rendimentos satisfatórios (98-30%) e em reduzido tempo de reação (5-20 min). O screening destas cumarinas frente às cepas do fungo S. schenckii revelou que 5-carboxi-6,7-diidroxi-4-metilcumarina, com CIM de 66 μM e Concentração Fungicida Mínima de 246 μM, foi o composto mais ativo. Essa cumarina apresentou sinergismo com a Anfotericina B, sendo sua CIM reduzida para 15 μM. A atividade antifúngica desses compostos pode estar correlacionada com a atividade antioxidante. O composto 5-carboxi-6,7-diidroxi-4-metilcumarina foi o mais ativo mostrando elevada capacidade antioxidante frente aos radicais DPPH com valores de IC50 de 17,49 μM e elevada atividade frente ao radical peroxil. Além disso, apresentou um potencial de oxidação de 0,4 V sugerindo atividade antioxidante. Baseado nos ensaios antioxidante e antifúngico foi possível observar que a presença de grupamentos hidroxilas no C-7 e C-8 do anel cumarínico, assim como a adição de grupamento polar no C-5 favoreceu ambas as atividades antifúngica e antioxidante. Na segunda etapa desse trabalho foi avaliada a citotoxicidade das cumarinas isoladas e algumas cumarinas simples, disponíveis comercialmente, pelo método Metil Tiazol Tetrazólio (MTT) usando linhagens de glioma humano (U138-MG) e de ratos (C6). Foi observado que as 6,7-metilenodioxicumarinas causaram uma significativa redução na viabilidade celular, sugerindo uma influência positiva do grupamento metilenodioxi sobre essa atividade. O composto 5-metóxi-6,7-metilenodioxicumarina foi o mais promissor (IC50= 34,6 μM e IC50= 31,6 μM para C6 e U-138 MG, respectivamente). Como desfecho desse trabalho, pode-se concluir que as cumarinas apresentaram uma atividade inibitória frente ao crescimento celular de linhagens de glioma e um efeito fungicida sobre S. schenckii, resultados estes que corroboram com o uso popular dessas plantas. / Coumarins are promising structures and diverse biological activities have been attributed to these secondary plant metabolites. Studies suggest that the mechanism of antifungal compounds is correlated with antioxidant activity. The reaction of tyrosinase, which produces free radicals, is involved in the process of melanization of the fungus Sporothrix schenckii, the agent of subcutaneous mycosis with the highest incidence in southern Brazil. The inhibition of this enzyme has recently been reported to extract Pterocaulon (Asteraceae) This plant is rich in coumarins and used in traditional medicine in Brazil for topical treatment of fungal infections and in folk medicine of Argentina as anticancer. The aim of this study was to investigate the in vitro antifungal activity and correlation with its antioxidant properties and cytotoxic activities of extracts of some species of Pterocaulon, as well as the isolated coumarins, and 4-methylcoumarin analogs obtained by synthesis. The 6.7-methylenedioxycoumarins were isolated from P. balansae and P. lorentzii, while the 4-methylcoumarins were synthesized via Pechmann reaction using microwave. The antifungal activity against Sporothrix schenckii was performed as indicated in the Manual of Clinical and Laboratory Standards Institute. A study of the electrochemical properties of coumarins was performed by cyclic voltammetry, by the method of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and against peroxyl radicals (ACAP) by fluorometric method. The analysis showed that the antifungal activity of the methanol extracts of Pterocaulon species (P. polystachyum, P. balansae, P. lorentzii, P. lanatum and P. cordobense) were active against the strains of the fungus S. schenckii, being the extract of P. polystachyum the most active, presenting Minimum Inhibitory Concentration (MIC) compressed between 156 and 312 μg/mL. The fractionation of lipophilic extracts of P. balansae and P. lorentzii led to the isolation of three methylenedioxycoumarins. The crystallographic data of 5-methoxy-6,7-methylenedioxycoumarin were deposited at the Cambridge Crystallographic Data Center 779123. The synthetic coumarins were obtained in satisfactory yields (98-30%) and reduced reaction time (5-20 min). The screening of these coumarins against strains of the fungus S. schenckii revealed that the 5-carboxy-6,7-dihydroxy-4-methylcoumarin was the most active compound, presenting MIC of 66 μM and Minimum Fungicidal Concentration of 246 μM. This coumarin showed synergism with Amphotericin B, and its MIC was reduced to 15 μM. The antifungal activity of phenolic compounds could be related to its antioxidant activities. The compound 5-carboxy-6,7-dihydroxy-4-methylcoumarin was again the most active with IC50 value of 17.49 μM, showing the highest capacity to deplete the radicals DPPH and ACAP, moreover present a oxidation potential of 0.4 V suggesting antioxidant activity. Based on the antioxidant and antifungal tests it was observed that the presence of hydroxyl groups at C-7 and C-8 of the coumarin ring and the addition of polar grouping at C-5 favored both antifungal and antioxidant activities.In the second part of this work, it was evaluated the cytotoxicity of the Pterocaulon compounds and some commercially available coumarins, with simple structure. The cytotoxic potential was determined by Methyl Thiazole Tetrazolium (MTT) test using strains of human glioma (U138-MG) and rat (C6). It was observed that the 6,7-methylenedioxycoumarins caused a significant reduction in cell viability, suggesting a positive influence of the methylenedioxy group in this activity. The compound 5-methoxy-6,7-methylenedioxycoumarin was the most promising (IC50 = 34.6 μM and IC50 = 31.6 μM for C6 and U-138 MG, respectively). In conclusion, this work it was demonstrated that some coumarins showed an inhibitory activity against the growth of glioma cell lines and a fungicidal effect on the S. schenckii. These results corroborate the popular use of these plants.

Made available in DSpace on 2015-07-22T20:50:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-11-24 / O rápido e intenso envelhecimento da população no Brasil e no mundo parece ser consequência de um melhor estado de saúde que se consegue durante certas fases da existência. Entretanto, a necessidade de manter a autonomia e a independência do idoso possui uma relação pertinente com os níveis de atividade física. Objetivo: Esta pesquisa tem por objetivo analisar e identificar o nível de atividade física e fatores associados, em especial nos domínios do transporte e no domínio lazer dos idosos residentes na zona urbana de cidade de Maceió, AL. Método: Para tanto, buscou-se fazer um estudo de delineamento transversal, de base populacional, com uma amostra de 319 idosos, sendo 97 do gênero masculino e 222 do gênero feminino; a média de idade foi de 69,24 anos (± 7.12 anos), com um valor mínimo de 60 anos e o máximo de 92 anos. Para avaliação da atividade física, particularmente nos domínios transporte e lazer, foi aplicado o “Questionário Internacional de Atividade Física” versão longa, tendo o ponto de corte em 150 minutos/semana, e para avaliação da saúde percebida foi utilizada a Escala de Saúde Percebida de De Vitta (2001). Os dados foram analisados por meio de análise descritiva, teste de Fisher e análise de regressão múltipla da razão de prevalência, sendo o nível de significância adotado de p<0,05. Resultados: Foram classificados como insuficientemente ativo no transporte 87,5%; no lazer, 76,2% dos idosos. A atividade física no transporte, considerada insuficiente, foi significativa entre os idosos com idades mais avançadas, por ter maior escolaridade, e considerar-se insatisfeito com a saúde física comparada na análise, ajustada nos dois modelos (modelo demográfico; modelo demográfico/saúde percebida). No lazer, a atividade física feita de forma insuficiente foi significante nas mulheres, nos homens com idade avançada, nos idosos com menor renda per capita, ao relatar a saúde física comparada e a autopercepção da saúde mental de insatisfeito nos dois modelos ajustados. Conclusão: Os resultados deste estudo apontam para uma alta prevalência de idosos insuficientemente ativos no transporte e no lazer. Nesse sentido, o estudo favorece a realização de novas pesquisas, com a finalidade de identificar as possíveis barreiras pessoais e/ou ambientais para prática da atividade física, e com consequente promoção de atividade física para os idosos de Maceió. / The fast and intense aging of the population in Brazil and in the world seems to be a consequence of a better level of health that happens during certain phases of the existence. However, the necessity to keep the autonomy and the independence of the aged ones has a pertinent relation with their levels of physical activity. Aim: to analyse and identify the level of physical activity and associated factors, in special in the domain of the transports and leisure activities of the aged residents in the urban zone of Maceió. Methods: The applied methodology is the transverse delineation, in a population base, with a sample of 319 old people, 97 men and 222 women, the average age was 69,24 years (± 7.12 years). With a minimum value of 60 years and the maximum one of 92 years old. For evaluation of the physical activity, in special in the domain of transport and leisure, it was applied the "International Questionnaire of Physical Activity" in its long version, with the cutoff point in 150 minutes/week and for evaluation of the health, it was used the Health Scale of De Vitta (2001). The data was analysed through descriptive analysis, Fisher test and analysis of multiple regression of the predominance reason, being the level of signification adopted p <0,05. Results: 87,5% of the old people researched were classified as insufficiently active in the transport area and 76.2% were considered insufficiently in the leisure area. The physical activity in the transport, was considered insufficient, but was significant among the elders with more advanced ages, due to higher schooling and it was considered dissatisfied in the physical health, compared in the analysis, adjusted in the two models (demographic model; demographic model / perceived health). In the leisure area, the physical activity done in an insufficient form was significant among women, with advanced age, in the elders with less per capita income, while reporting the compared physical health and the auto perception of the mental health it was dissatisfied in the two adjusted models. Conclusion: the results of this study point to a high predominance of insufficiently active elders in the transport activity and in the leisure time. This study favors the achievement of new researches, with the purpose of identify the possible personal or environmental barriers for practicing the physical activity, with consequent promotion of physical activity for the elders of Maceió. / TEDE

Nos últimos anos, os metabólitos isolados de fungos marinhos vêm ganhando considerável atenção em razão de possuírem estruturas químicas únicas com diversas atividades biológicas já descritas, incentivando novas pesquisas na área. A tricomoníase é a doença sexualmente transmissível (DST) de origem não viral mais comum no mundo, estando associada a sérias consequências à saúde, sendo relatado um aumento no número de isolados clínicos resistentes ao tratamento de escolha. Infecções causadas por bactérias com diferentes mecanismos de resistência, representam um grande desafio para a saúde pública atual, acarretando em altas taxas de mortalidade e morbidade. As bactérias patogênicas dispõem de fatores de virulência, como a formação de biofilme, que agravam infecções tornando-as persistentes. Devido ao potencial biológico dos produtos de origem marinha e a importância dessas infecções, o objetivo deste estudo foi avaliar a atividade anti-T. vaginalis, antimicrobiana e antibiofilme de moléculas obtidas da fermentação de fungos associados a organismos marinhos. As 14 cepas fúngicas foram isoladas de esponjas e corais marinhos, obtidos da costa do estado de Alagoas, Brasil. Após produção do metabólito, o micélio foi separado do meio. O micélio foi extraído com metanol e o meio com acetato de etila. As frações foram submetidas aos ensaios de atividade anti-T.vaginalis, antimicrobiana e antibiofilme (inibição da formação e erradicação). As frações que demonstraram atividade foram submetidas ao ensaio de hemólise, avaliação da citotoxicidade (HMVII e Vero) e toxicidade em modelo de Galleria mellonella. A fração que demonstrou os melhores resultados nos ensaios de atividade foi submetida ao fracionamento bioguiado. As frações orgânicas dos fungos Aspergillus niger (FMPV 03) e complexo Trichoderma harzianum/Hypocrea lixii (FMPV 09) foram ativas frente ao T. vaginalis ATCC 30236, com valores de MIC de 2 mg/mL e 1 mg/mL, respectivamente. Quando investigadas, essas frações mantiveram a atividade frente ao isolado clínico resistente ao metronidazol (TV-LACM2R), apresentado os mesmo valores de MIC encontrados para o isolado ATCC. Para a atividade antimicrobiana, as frações orgânicas do Aspergillus niger (FMPV 03), Aspergillus tubingensis (FMPV 06), complexo Trichoderma harzianu/Hypocrea lixii (FMPV 09) e Aspergillus sydowii (FMPV 10) foram ativas contra S. epidermidis ATCC 35984. Ainda, frente a P. aeruginosa ATCC 27853 somente as frações orgânicas do Aspergillus niger (FMPV 03) e Aspergillus tubingensis (FMPV 06) demonstraram atividade. Neste ensaio, para ambas as bactérias, os valores de MIC não ultrapassaram 1,5 mg/mL. A atividade destas frações também foi observada frente às mesmas bactérias no ensaio de antiformação de biofilme, já que ocorreu a morte das células. A habilidade de erradicar biofilmes foi detectada somente para a fração orgânica do fungo Aspergillus flavus (FMPV 01), o qual foi capaz de remover 52% do biofilme já formado de S. epidermidis ATCC 35984. A ausência de hemólise dos eritrócitos foi observada em todas as frações ativas estudadas. Na avaliação da citotoxicidade in vitro frente às linhagens celulares HMVII e Vero, apenas a fração orgânica do Aspergillus niger (FMPV 03), não apresentou efeito citotóxico. No entanto, no ensaio de avaliação da toxicidade in vivo, nenhuma das amostras testadas causou redução na sobrevivência das larvas de Galleria mellonella. Então, a fração orgânica do fungo Aspergillus niger (FMPV 03) foi submetida ao fracionamento bioguiado utilizando coluna RP-18. Sete frações foram obtidas, sendo que a primeira (100% água), foi ativa contra T. vaginalis, S. epidermidis e P. aeruginosa. Quando submetida à Cromatografia em Camada Delgada (CCD), demonstrou quatro bandas que foram coradas com anisaldeído-ácido sulfúrico e ninhidrina. Além disso, a fração 100% água não demonstrou redução da sobrevivência das larvas de Galleria mellonella, nas três concentrações testadas. Portanto, a gama de atividades relatadas corrobora o potencial dos fungos marinhos na produção de moléculas bioativas. / In recent years, the isolated metabolites from marine fungi have been attracted considerable attention due to unique chemical structures with diverse biological activities, encouraging further research in the area. Trichomoniasis is the most prevalent non-viral sexually transmitted disease (STD) worldwide. This has been linked to serious health consequences and an increase in the number of clinical isolates resistant to the treatment of choice has been reported. Infections caused by bacteria with different resistance mechanisms represent a major challenge to the current public health, resulting in high rates of mortality and morbidity. Pathogenic bacteria present virulence factors, such as biofilm formation, which migth enhance the persistence of infections. Due to the biological potential of marine products and the importance of these infections, the aim of this study was to evaluate the anti-T. vaginalis, antimicrobial and antibiofilm activities of the obtained molecule from the fermentation of fungi associated with marine organism. The 14 fungal strains were isolated from sponges and corals marine obtained from the coast of Alagoas, Brazil. After production of the metabolite the mycelium was separated from the medium. The mycelium was extracted with methanol and the medium with ethyl acetate. The fractions were subjected to the assays anti-T. vaginalis, antimicrobial and antibiofilm (inhibition of the formation and eradication) activities. The fractions that showed activity were subjected to the assays of hemolysis, cytotoxicity evaluation (HMVII and Vero) and toxicity Galleria mellonella model. The fraction which showed the best results in activity assays was subjected to fractionation bioguided. The organic fraction of Aspergillus niger (FMPV 03) and Trichoderma harzianum/Hypocrea lixii complex (FMPV 09) were active against T. vaginalis ATCC 30236 with MIC values of 2 mg/mL and 1 mg/mL, respectively. When investigated, these fractions maintained activity against resistant clinical isolate to metronidazole (TV-LACM2R), presented the same MIC values found to isolate ATCC. For the antimicrobial activity, the organic fractions of Aspergillus niger (FMPV 03), Aspergillus tubingensis (FMPV 06), Trichoderma harzianum/Hypocrea lixii complex (FMPV 09) and Aspergillus sydowii (FMPV 10) were active against S. epidermidis ATCC 35984. Even, against P. aeruginosa ATCC 27853 only the organic fractions of Aspergillus niger (FMPV 03) and Aspergillus tubingensis (FMPV 06) demonstrated activity. In this test, for both bacteria, MIC values did not exceed 1.5 mg/mL. The activity of these fractions was also observed across the same bacteria in the antibiofilm formation assay, since cell death occurred. The ability to eradicate biofilms was detected only for the organic fraction of the fungus Aspergillus flavus (FMPV 01) which was able to remove 52% of the already formed biofilm of S. epidermidis ATCC 35984. The absence of hemolysis of red cells was observed in all active fractions studied. In the assessment of cytotoxicity in vitro against the cell lines HMVII and Vero, only the organic fraction of Aspergillus niger (FMPV 03), showed no cytotoxic effect. However, in the test evaluation of in vivo toxicity, none of the samples tested caused a reduction in the survival of the larvae of Galleria mellonella. Then, the organic fraction of the fungus Aspergillus niger (FMPV 03) was submitted to bioguided fractionation using RP-18 column. Seven fractions were obtained, of which the first (100% water), was active against T. vaginalis, S. epidermidis e P. aruginosa. When subjected to thin layer chromatography (TLC) showed four bands were stained with ninhydrin and anisaldehyde-sulfuric acid. In addition, 100% water fraction showed no reduction of the survival of larvae of Galleria mellonella at the three concentrations tested. Therefore, the range of activities reported corroborates the potential of marine fungi to produce bioactive molecules.

A significant problem of the 21st century, across different countries, is currently insufficient physical activity and a large increase in obesity . Influence of physical impairment on the prevalence of obesity is proven. At this time the physical activity is not sufficient for biological needs of man and as a result of this is a threat to the human species. With the alarming rise of health problems, which results from improper lifestyle , against all the progress in medical science, these conditions are a reality of modern civilization. Physical activity with its health benefits is one of the key topics of health education. In this thesis, the state of physical activity among female students of The Faculty of Education, University of South Bohemia in Ceske Budejovice, both day and part-time bachelor's and master's degree program is monitored by using the International Physical Activity Questionnaire. The theoretical part deals with concepts such as physical activity and a summary of the activities practiced out in a unit of time and definition and classification of them. Another two chapters are devoted to the health benefits of physical activity and another aspects of physical activity in relation to health. The practical part of this thesis analyzes in detail the results of the standardized questionnaire IPAQ, which monitors the activity of the individual within seven days. Female Students responded to 27 questions , which were divided into four part evaluation exercise. A separate part of the evaluation was the time spent sitting and personal anamnesis of the respondent. The evaluation was processed using simple statistical method and recorded in a bar graph.