Epilobium hirsutum L. (Onagraceae) is a flowering, tall and perennial plant and native to Eurasia. It shows analgesic, anti-microbial and anti-proliferative activity, and it is used in our country as an alternative medicine. The pharmacological effect of Epilobium hirsutum L. could be explained by the presence of polyphenolics including steroids, tannins and flavonoids in the aerial parts. Viscum album L. (Loranthaceae) is a shrub that grows as an epiphyte on the branches of deciduous trees. It involves in the enhancement of macrophage phagocytic and cytotoxic mediated abilities as well as the strengthening the immune system. CYP24A1 and CYP27B1 are members of cytochrome P450 superfamily and the most important enzymes involved in the metabolism of vitamin D3. CYP27B1 and CYP24A1 are mitochondrial enzymes and also known as 25-hydroxyvitamin D3 1alpha-hydroxylase and 24-hydroxylase, respectively. CYP24A1 involves in 24-hydroxylation of 25-OH-D3 and 1,25-(OH)2D3 which is required for the catabolism of vitamin D3 compounds while CYP27B1 involves in 1&alpha / -hydroxylation of 25-OH-D3 into 1,25-(OH)2D3. In this study, in vivo effects of Epilobium hirsutum and Viscum album (subspecies growing on pine-trees-subsp. austriacum (Wiesb.) Vollmann) on rat liver CYP24A1 and CYP27B1 mRNA and protein expressions were investigated. To achieve this goal, 37.5 mg water extract of Epilobium hirsutum L./kg body weight/day was intraperitoneally injected to male rats for 9 days. To study the effect of Viscum album L., 10 mg water extract of Viscum album L./kg body weight/day was injected with the same conditions. After decapitation, livers were removed and S1.5 fractions were prepared. Effects of Epilobium hirsutum L. and Viscum album L. on rat liver mRNA and protein expressions were analyzed by qRT-PCR and western blotting, respectively. Epilobium hirsutum L. extract caused 31% and 18% decrease in rat liver CYP24A1 (p&lt / 0.0001) and CYP27B1 (p&lt / 0.05) protein expressions, respectively. The effect of Epilobium hirsutum L. on mRNA expression of CYP24A1 could not be observed, because CYP24A1 mRNA was almost undetectable in liver. Injection of Epilobium hirsutum L. to rats caused 2.7 fold increase in mRNA expression of CYP27B1 with respect to controls and normalized with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) expression as an internal reference (p&lt / 0.005). Viscum album L. caused 17% decrease in CYP24A1 protein expression (p&lt / 0.05). When rats injected with plant extract of Viscum album L., 18% decrease in CYP27B1 protein expression was observed (p&lt / 0.05). The effect of Viscum album L. on mRNA expression of CYP24A1 could not be observed since CYP24A1 mRNA was almost undetectable in liver. Injection of Viscum album L. to rats caused 3.8 fold increase in mRNA expression of CYP27B1 with respect to controls and normalized with GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) expression as an internal reference (p&lt / 0.005). In conclusion, vitamin D3 metabolism may be affected by medicinal plants Epilobium hirsutum L. and Viscum album L. due to the changes in mRNA and protein expressions of CYP24A1 and CYP27B1 enzymes.

Epilobium hirsutum L. (Onagraceae), a medicinal plant known as hairy willow herb, has been used by people all around the world for treatment or prevention of inflammation, adenoma, rectal bleeding, menstrual disorders, constipates, and prostate. It contains polyphenolics including steroids, tannins such as gallic, ellagic, and p-coumaric acids and flavonoids such as myricetin, isomyricetin, and quercetin. Polyphenols have been known for their multiple biological health benefits, including antioxidant activities. Viscum album L. (Loranthaceae), a species of mistletoe, contains lectins, polypeptides, mucilage, sugar alcohols, flavonoids, lignans, triterpenes, and phenylallyl alcohols. The leaves and twigs of Viscum album L., taken as tea, have been traditionally used for hypertension, stomachache, diarrhea, diabetes, dysuria and also as analgesic and cardiotonic agent in Anatolia, Turkey. In addition, in Europe, sterile extracts of Viscum album L. are among the most common herbal extracts applied in cancer treatment and have been used as prescription drugs, while in US, considered as dietary supplement. Flavin-containing monooxygenases are FAD-containing phase I enzymes responsible for the oxidation of wide-range of nucleophilic nitrogen, sulfur, phosphorus, and selenium heteroatom-containing drugs such as tamoxifen, v methimazole and imipramine, pesticides, neurotoxins, and other chemicals using NADPH as cofactor. The aim of this study was to determine the in vivo effects of Epilobium hirsutum L. and Viscum album L. (subspecies growing on pine trees-subsp. austriacum (Wiesb.) Vollmann) on FMO activity, mRNA and protein expressions in rat liver. The water extracts of Epilobium hirsutum L. (37.5 mg/kg body weight) and Viscum album L. (10 mg/kg body weight) were injected intraperitonally (i.p) into Wistar albino rats for 9 consecutive days. Following the decapitation, the livers were removed and microsomal fractions were prepared by differential centrifugation. Rat liver microsomal FMO activity using methimazole as substrate, mRNA expression by quantitative Real-Time PCR, and protein expression by Western Blot were determined. The results showed that water extract of Epilobium hirsutum L. has no significant effect on FMO activity / however, it decreased significantly (p&lt / 0.05) FMO3 protein and mRNA expression 27.71% and 1.41 fold, respectively, compared as controls. Water extract of Viscum album L. decreased mRNA (2.56 fold), and protein expressions (27.66%) as well as enzyme activity (19%) of FMO with respect to controls. In conclusion, our current data suggest that the metabolism of xenobiotics including drug molecules by FMO-catalyzed reactions may be altered due to the changes in FMO expression and activity by medicinal plants Epilobium hirsutum L. and Viscum album L.

Calcium ion plays a pivotal role as second messenger during signal/response coupling in plant cells (Trewavas, 1999). Elevations of cytosolic Ca2+ occur in plants as a consequence of abiotic and biotic stresses, environmental and hormonal stimuli. However, the molecular mechanism by which changes in cytosolic calcium are sensed and transduced in the plant cell has not been completely elucidated. The detection of Ca2+-binding proteins, especially Ca2+-dependent protein kinases (CDPKs) in plants led to drawing analogy with animal systems wherein the Ca2+-message is perceived and transduced by proteins that bind Ca2+. CDPKs are stimulated by the direct binding of Ca2+ to their endogenous calmodulin (CaM) -like domain (Harper et al, 1991). CDPKs exist as multiple isoforms in a single species, and show tissue-specific and developmentally regulated expression. Furthermore, the diversity among different CDPK isoforms with respect to Ca2+-binding properties, activation, substrate specificity, regulatory mechanisms and other kinetic properties suggest their specialization in the regulation of distinct signaling pathways. These observations therefore have led to the speculation that most of the Ca2+-mediated signal transduction in plants occurs via the mediation of CDPKs (Harmon et al, 2000). Over the last 15 years there has been a dramatic unfolding of information on Ca2+-mediated signaling in plants. Nevertheless, little is known about the environmental/hormonal signals and the signaling events that regulate early plant developmental processes such as embryogenesis, seed development and germination. The present investigation was initiated with the objectives 1) to determine the role of Ca2+ during embryogenesis, 2) to examine the involvement of a CDPK during early developmental processes in sandalwood plant (Santalum album L.) and 3) to purify and biochemically characterize this CDPK. The study initially investigated the possible involvement of calcium-mediated signaling in the induction/regulation of somatic embryogenesis from proembryogenic cells of sandalwood. 45 Ca + uptake studies and fura-2 fluorescence ratio photometry were used to measure changes in [Ca2+]cyt of proembryogenic cells in response to culture conditions conducive for embryo development. Sandalwood proembryogenic cell masses (PEMs) were obtained in the callus proliferation medium that contains the auxin 2,4-D. Subculture of PEMs into the embryo differentiation medium which lacks 2,4-D and has higher osmoticum resulted in a 4-fold higher 45Ca2+ incorporation into the symplast. Fura-2 based ratiometric analysis also showed a 10-16- fold increase in the [Ca2+]cyt of PEMs under identical culture conditions, increasing from a resting concentration of 30-50 nM to 650-800 nM. Chelation of exogenous Ca2+ with EGTA arrested such an elevation in [Ca2+]cyt. Exogenous Ca2+ when chelated or deprived also arrested embryo development and inhibited the accumulation of a Ca2+-dependent protein kinase (swCDPK) in embryogenic cultures. However, such culture conditions did not cause cell death as the PEMs continued to proliferate to form larger cell clumps. Culture treatment with W7 reduced embryogenic frequency by 85%, indicating that blockage of Ca2+-mediated signaling pathway(s) involving swCDPK and/or CaM caused inhibition of embryogenesis. These observations suggest a second messenger role for exogenous Ca2+ and the existence of Ca2+-mediated signaling pathway(s) during sandalwood somatic embryogenesis. The detection of a 55 kD protein showing cross reactivity with polyclonal antisoybean CDPK and the detection of Ca2+-dependent protein kinase activity in protein extracts from somatic embryos, prompted investigation on the spatio-temporal accumulation and activity of a CDPK in different developmental stages of sandalwood. Western blot analysis and protein kinase assays identified a Ca2+-dependent protein kinase (swCDPK) of 55 kD in soluble protein extracts of different developmental stages of sandalwood somatic embryos. However, swCDPK was not detected in plantlets regenerated from somatic embryos. swCDPK exhibited differential expression and activity in the developmental stages of sandalwood. Zygotic embryos, endosperm and seedlings showed high accumulation of swCDPK. However, the enzyme was not detected in the soluble proteins of shoots and flowers of sandalwood tree. swCDPK exhibited a temporal pattern of expression in endosperm, showing high accumulation and activity in mature fruit and germinating stages, the enzyme being localized strongly in the storage bodies of the endosperm cells. Interestingly, these storage bodies were thereafter identified as oil bodies, suggesting that a Ca2+-mediated regulation of oil hydrolysis and/or mobilization might be operative during seed germination. swCDPK in the zygotic embryo was found to be inactive during seed dormancy and early stages of germination, indicating a possible post-translational hibition/inactivation of the enzyme during these stages. The temporal expression of swCDPK during somatic/zygotic embryogenesis, seed maturation and germination thus suggests involvement of the enzyme in these early developmental processes. In view of the diversity exhibited by members of the CDPK family, characterization of swCDPK, the early development specific CDPK from sandalwood was undertaken. Purification of swCDPK was achieved by chromatography on DEAE-cellulose, hydroxyapatite and Blue-Sepharose. The purified enzyme resolved into a single band on 10 % polyacrylamide gels, both under denaturing and non-denaturing conditions. swCDPK was strictly dependent on Ca2+, K0.5 (apparent binding constant) for Ca2+-activation of substrate phosphorylation activity being 0.7 μM and for autophosphorylation activity —50 nM. Ca2+-dependence for activation, CaM-independence, inhibition by CaM-antagonist (IC50 for W7 = 6 μM, for W5 = 46 μM) and cross-reaction with polyclonal antibodies directed against the CaM-like domain of soybean CDPK, confirmed the presence of an endogenous CaM-like domain in the purified enzyme. Kinetic studies revealed a Km value of 13 mg/mL for histone III-S and a Vmax of 0.1 nmolmin-1rng-1. The enzyme exhibited high specificity for ATP with a Km value of 10 nM. Titration with Ca2+ resulted in enhancement of the intrinsic emission fluorescence of swCDPK and a shift in the λmax emission from tryptophan residues. A reduction in the efficiency of non-radiative energy transfer from tyrosine to tryptophan residues was also observed. These are taken as evidence for the occurrence of Ca2+-induced conformational change in swCDPK. The emission spectral properties of swCDPK in conjunction with Ca2+ levels required for autophosphorylation and substrate phosphorylation help elucidate the possible mode of Ca2+ activation of this enzyme.

The timing of weed emergence is of major importance for integrated weed management programs. If accurately forecasted, the timing of weed control operations could be optimized, enabling the implementation of more effective control strategies and consequently, the reduction of herbicide use. Little is known about weed emergence in Quebec. Weed emergence in the field results from the breaking of seed dormancy, seed germination and growth of the seedling to the soil surface. The purpose of this study was initially to review the environmental and cultural factors that affect these mechanisms, to determine which factors are mainly responsible for weed emergence in southwestern Quebec and, finally to develop a weed emergence model. This study focused primarily on common lambsquarters (Chenopodium album L.) and barnyardgrass (Echinochloa crus-galli [L.] Beauv.) since they were the most abundant weeds, representing 37 and 22%, respectively, of the total number of annual weeds in the experimental sites. A 3-year study established that the presence of corn did not affect the density and the pattern of emergence of these weeds. A 2-year experiment demonstrated that rainfall and irrigation had no or little influence on the pattern of weed emergence since soil water content was at or greater than field capacity in the spring. In Quebec, temperature was determined to be the most important factor regulating weed germination and emergence, meaning that weed germination is initiated by a minimal temperature in the spring and that this temperature is specific to each species. Thermo-gradient plate experiments established a base temperature for common lambsquarters, redroot pigweed (Amaranthus retroflexus L.), barnyardgrass, and green foxtail (Setaria viridis L.) of 4, 8, 11, 12 +/- 1 C and <3, >16, 12 and 12 +/- 1 C, respectively for seed lots originating from eastern and southwestern Quebec. These results served as a starting point for the calculation of thermal units for the

<p>Abstract</p><p>The album phenomenon developed in France and Spain and lasted throughout the entire nineteenth century. Albums were books with blank pages in which the owner collected contributions in the form of poetry, drawings, and music scores. These works were created for album owners by friends, acquaintances, and sometimes even suitors, and were meant to pay tribute to them. It is possible to imagine the album as a space of intense social and economic rivalry, in which owners of the books competed with one another to obtain the most luxurious books, and to fill them with the greatest number of entries from renowned artists. Similarly, contributors implicitly competed with one another to create the highest quality entries and contribute to albums that advanced their status as artists. However, established writers did not need further publicity, and many complained about the hassle of the constant request for contributions. To highlight the scope of the album phenomenon, and the frustration it caused writers and artists, José Zorrilla denounced album entry requests, stating that he had been solicited for album contributions a total of 188,000 times in his life. Honoré de Balzac condemned the album fashion even more fervently, declaring: "To hell with all albums." </p><p>I study the album as a practice that provides important information regarding gender, economic, and artistic exchanges in the milieus in which it flourished. My approach is based on the study of different types of texts. First, I analyze three essays on social customs that present the album from a perspective that mixes journalism and satire: Victor-Joseph Etienne de Jouy's 1811 essays "Des Album" and "Recherches sur l'Album et sur le chiffonier sentimental," and Mariano José de Larra's 1835 essay "El album." I use these essays to formulate a contextual theory on the album. I also examine nineteenth-century albums that I consulted in archives in France and Spain. Studying both the material construction of the albums and the contributions included in them, I try to understand the social and economic determinants of this social custom. Through the album entries, I explore the artistic networks established through, and exploited by, the album phenomenon, which were essential for successfully collecting contributions. Finally, I analyze fictional texts in which the album serves as a pivotal plot element used to shape the development of the stories and the roles of the protagonists. In my analysis of literary texts that portray the album, I focus on the establishment of gender and economic exchanges in this practice. I explore the imposition of traditional gender roles in the album phenomenon, according to which women were exclusively album owners and men were contributors. In my analysis of fictional texts, I also examine the economic aspect of this practice, reflecting upon the social class of the fictional characters involved in it. The literary texts that I study are: Honoré de Balzac's La Muse du département (1837), Manuel Bretón de los Herreros' El poeta y la beneficiada. Comedia en dos actos (1838) and El cuarto de hora. Comedia en cinco actos (1848), Juan de Ariza's "Historia de un album" (1847), Henri de Meilhac's L'autographe. Comédie en un acte (1858), Antonio Flores' "Cuadro cincuenta y uno. Placeres de sobremesa" (1863), José María de Pereda's Pedro Sánchez (1883), Juan López Valdemoro's "El álbum" (1886), and Leopoldo Alas `Clarín''s "Album-abanico" (1898). </p><p>The nineteenth century saw the rise of consumer culture and the proliferation of objects, such as cardholders, parasols, fans, pocket watches, and other trinkets. The album is at once part of this plethora of nineteenth-century objects and yet it is also distinct, in that it was a special piece of material culture that promoted a particular type of personal communication and required the creation of textual production. The album was established as a unique cultural manifestation, the study of which allows for a reconstruction of different types of social dynamics in its milieu. </p><p>Due to the complexity and richness of this object-centered practice, and the ways in which it developed, the album offers multiple analytical possibilities, as a social, historical, and literary phenomenon. One of the most significant contributions of this project lies in its transnational perspective and in its comparative analysis of different types of texts: essays on social customs, literary texts, and personal collections that survive in archival albums. The study of the exchanges that were fostered, and capitalized upon, through the album fashion is essential for understanding notions of private and public and collection as a practice. My analysis of the album yields invaluable insights into gender and class dynamics, ideas of art, and visual and material culture in nineteenth-century France and Spain.</p> / Dissertation

Cette thèse s’inscrit dans une problématique générale de la littérature de jeunesse comme lieu d’exigence et d’émergence des formes, lieu d’entrée de l’enfant en littérature. L’étude porte sur l’album contemporain, plus spécifiquement sur l’oeuvre foisonnante de Béatrice Poncelet, novatrice tant par ses modes de dialogue « texte-image » que par ses choix énonciatifs. L’album, s’il paraît à première vue relever du narratif, a aussi à voir avec le poétique, voire même le théâtral, du fait de sa plasticité, de ses jeux avec la matérialité de la langue, comme autant d’indices de la modernité de cette littérature contemporaine. Développant son propre système critique à la croisée des genres, l’album complexe ouvre à des expériences de lecture et des interprétations plurielles. Dans une perspective didactique et face au décalage parfois évident entre les goûts des lecteurs experts, des enseignants et des enfants, nous nous interrogeons ici sur la réception par les enfants et les adultes des albums de Béatrice Poncelet, sur la nécessaire médiation de l’adulte et sur la vocation de l’école à amener les enfants vers ce genre de production. L’enquête menée auprès d’élèves et d’enseignants de l’école primaire et de SEGPA nous conduit alors à faire l’hypothèse que c’est précisément la rencontre avec les supports complexes qui permet aux élèves, y compris disqualifiés en lecture, de se constituer comme sujets lecteurs et, partant, à envisager des dispositifs de formation à la littérature et par la littérature, pour les élèves et pour les maîtres. / This dissertation is situated within a general approach of youth literature not only as a first entry into literature for children, but also as the site of emerging forms of exacting standards. The study concerns contemporary albums, especially the prolific works of Beatrice Poncelet, whose innovations concern both the modes of dialogue between text and image and her choices in terms of enunciation. While at first sight the albums seem more narrative, they are equally poetic, even theatrical; along with the plasticity of their games with the materiality of language, these all point to the modernity of this contemporary writing. In developing their own critical system at the crossroad of genres, the complex albums open to a variety of reading experiences and interpretations. In a didactic perspective, and confronted to the gap – at times quite manifest – between the tastes of expert readers, teachers, and children, this study focuses on the reception by adults as well as children of Beatrice Poncelet’s albums, and on the necessary mediation by adults: the school context is required to introduce this type or genre of literary production to the children. The survey conducted with primary school teachers and pupils, as well as professional classes (SEGPA), leads to the following hypothesis: it is precisely through such encounters with complex reading materials that school children, even those who are least recognized as readers, to develop as reading subjects. This leads to a need for programs training both students and teachers in literature and through literature.

This thesis examines the ways in which Beyoncé Knowles-Carter’s 2016 album Lemonade works as a culturally significant text in the realm of popular media. Situated within Black feminist theoretical concepts of freedom practices and Black Feminist Love Politics, the thesis argues that Lemonade mobilizes stylistic and strategic intertextual references to develop a transmedia storyworld within a paradigm of resistance to, and healing from, white supremacist histories. Such intertextual information exists within the musical, lyrical, visual, poetic, and transmedia domains of Lemonade. The transmedia extensions include interviews, live performances, speeches, social media posts, and photoshoots. Combined with theories from Black feminist thought of freedom practices—which include talking back (bell hooks 1989), dark sousveillance (Simone Browne 2015), and interruptions to whiteness (DiAngelo 2011)— and Black Feminist Love Politics (Jennifer Nash 2013), the intertextual data present in Lemonade can be analyzed using methodologies from the field of popular musicology (intertextuality and mediality).

[pt] Em diversos países europeus, norte e latino-americanos estão disseminados entre especialistas termos que representam um tipo específico de subcategoria da literatura infantil, como picturebook, album illustré, álbum ilustrado, livro-álbum e bilderbuch, caracterizado pelo papel que a linguagem visual representa na leitura da obra. Apesar de não haver um nome específico para tal subcategoria no Brasil, há muitos exemplos de livros ilustrados brasileiros que aqui a representam. Esta pesquisa trata sobre tal objeto analisando-o sob três aspectos: primeiro, suas definições por diferentes autores brasileiros e estrangeiros, com foco nas especificidades do diálogo entre linguagens verbal e visual e uso consciente da tecnologia livro como recurso estético; segundo, em uma análise da infância sob abordagem filosófica e social, em busca dos pressupostos de leitura que o livro ilustrado, ao ser considerado um livro infantil, pode produzir; e terceiro, sobre as particularidades da criação de um objeto que muitas vezes é fruto de quatro autores - o escritor, o ilustrador, o designer e, por vezes, o editor. / [en] In many countries from Europe, North and Latin America there are different terms commonly used by specialists to refer to a certain subcategory of children s literature - picturebook, album illustré, álbum ilustrado, livro-álbum, and bilderbuch - characterized by the role that visual language plays in reading of the work. Despite the want of a specific name for this subcategory in Brazil, there are many examples of Brazilian picturebooks. This research focuses on the picturebook, and analyzes it from three different perspectives: firstly, definitions presented by different Brazilian and foreign authors, with focus on the specificities of the dialog between verbal and visual languages, and the deliberate use of the book technology as an aesthetic object; secondly, analyzing childhood under a philosophical and sociological approach in search of the possible implications to reading that the categorization of the picturebook as children s book may produce; thirdly, the particularities of creating an object which often has four authors - the writer, the illustrator, the designer, and the editor.

Made available in DSpace on 2015-05-14T12:39:51Z (GMT). No. of bitstreams: 1 arquivototal.pdf: 2359059 bytes, checksum: 63873d1da8ab460889db4579b9db0d7b (MD5) Previous issue date: 2013-03-21 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / Tropicalia emerged in late 1967 with the songs ―Alegria, Alegria‖ (Caetano Veloso) and ―Domingo no Parque‖ (Gilberto Gil). But it was in 1968 that the musical movement left his major works recorded. In that year were released records by Tom Zé, Os Mutantes, Caetano Veloso, Gilberto Gil and the collective record Tropicália ou panis et circensis. These albums represented a new moment in the Brazilian Music: opening the explicit cultural dialogues with other countries and with different artistic languages. Our work investigates how was, parallely, turned towards the Brazilian graphic design, from the analysis of the covers of these records, discussing the images with the songs. We started from Luiz Tatit s studies on song and the concept of cultural dialogue according to the Semiotics of Culture. / A Tropicália surgiu no final de 1967, com as canções ―Alegria, alegria‖ (Caetano Veloso) e ―Domingo no parque‖ (Gilberto Gil). Mas foi em 1968 que o movimento musical deixou registrado em disco suas principais obras. Naquele ano foram lançados os discos de Tom Zé, Mutantes, Caetano Veloso, Gilberto Gil e o disco coletivo Tropicália ou panis et circensis. Estes álbuns representaram um novo momento na canção brasileira: da abertura explícita aos diálogos culturais com outros países e com diversas linguagens artísticas. Nosso trabalho investiga como se deu, paralelamente, a virada de rumo no design gráfico brasileiro, a partir da análise das capas destes discos, dialogando as imagens com as canções. Partimos dos estudos de Luiz Tatit sobre canção e do conceito de diálogo cultural segundo a Semiótica da Cultura.

This text talks about a family invited to face their family album. To do that, it was considered this time, in which families relation nets are (re) configured and established in another manner. In this way, due to the complexity of this theme, this album was considered as a liquid family album. These facts are pointed in order to cause curiosity about a family album and then reflect about it as a reason for other senses and knowledge as well as a dispositive of conversation in investigations related to education. From this perspective, it was done some inferences about a liquid family album that has among its images/senses/characters a subject with mental disability. This is because the main objective of this study consists in asking about the liquid family configuration and also about the place and non-place of the subject who has the mark mental disability in this relation net. With this investigation, it is proposed the (de) construction of the mark mental disability based on the configuration of the liquid family and the studies about this liquid time (ephemeral, volatile, in bitter (de) construction of concepts, marks, values, tendencies ), described by some scholars, specially by Zygmunt Bauman. Thus, it was talked about something that has been produced, that is product and produces contemporary time. / Este texto disserta a respeito de uma família convidada a um enfrentamento com o seu álbum de família. Para isso, levou-se em consideração este tempo, em que as redes de relações familiares (re) configuram-se e se estabelecem de outra maneira. Sendo assim, devido à complexidade dessa temática, considerou-se esse álbum como sendo um álbum de família líquida. Esses elementos são considerados a fim de curiosear um álbum de família, e, assim, problematizá-lo como disparador de sentidos outros e de conhecimento, bem como um dispositivo de conversação na investigação em educação. A partir dessa perspectiva, fizeram-se algumas inferências acerca de um álbum de família líquida que tem, dentre as/os suas/seus imagens/sentidos/personagens, um sujeito que carrega a marca deficiência mental. Isso porque, o propósito deste estudo consiste em se perguntar sobre a configuração de família líquida e o lugar ou não-lugar do sujeito que carrega a marca deficiência mental nesta rede de relações. Com esta investigação, propõe-se a (des) construção da marca deficiência mental, tendo em vista a configuração de família líquida e os estudos sobre este tempo líquido (efêmero, volátil, em crônica (des) construção de conceitos, marcas, valores, tendências...), descrito por alguns teóricos, especialmente por Zygmunt Bauman. Desse modo, dissertou-se acerca de algo que está sendo produzido, é produto e produz a contemporaneidade.