京都大学 / 新制・課程博士 / 博士(理学) / 甲第24468号 / 理博第4967号 / 新制||理||1709(附属図書館) / 京都大学大学院理学研究科生物科学専攻 / (主査)准教授 Huffman Michael Alan, 教授 古市 剛史, 教授 今井 啓雄 / 学位規則第4条第1項該当 / Doctor of Science / Kyoto University / DFAM

The purpose of this dissertation is first to provide an overview of professional competency development and teaching in veterinary education, and then to address the dearth of research in this area by examining students' motivational perceptions of their courses, how these perceptions compare and contrast across courses, and how their perceptions relate to their effort levels and course ratings. Professional competencies encompass cognitive, social, and personal resource skills such as interpersonal communication skills, collaboration, management, promotion of public health, lifelong learning, ethics, diversity competence, and adaptability to changing environments. The inclusion of professional competencies as a requisite portion of the veterinary curriculum has evolved substantially over the past 20 years. In this dissertation, two manuscripts are presented. The first (Chapter 2) is a literature review exploring the past 20 years of professional competency teaching and its development within, and inclusion into, the veterinary curriculum. The first manuscript concludes by describing recommendations from the literature for effective methods of inclusion of professional competencies into the veterinary curriculum. The second manuscript (Chapter 3) details a study conducted in a veterinary college with the aim of determining the extent to which students' motivational perceptions of their courses affect their effort and course ratings in veterinary courses. A second purpose of this study was to identify teaching strategies that can be used to improve the quality of teaching in professional competency courses. Results indicated that perceptions of empowerment, usefulness, and interest have the strongest relationship with effort and course ratings. Based on student responses to open-ended items, suggestions were made that instructors can use to improve student perceptions in their courses as a means to potentially increase student effort levels and overall course ratings. Together, these manuscripts contribute to current motivational theories and offer instructional design ideas to curriculum designers and educators who wish to improve students' motivation and engagement in professional competency development. / Doctor of Philosophy / The overall purpose of this dissertation was to explore student motivation within veterinary education with the goal of identifying ways to improve veterinary courses as well as students' willingness to put effort into their coursework. This study focused primarily on the teaching of professional competencies, which refers to skills in communication, collaboration, management, promotion of public health, lifelong learning, ethics, diversity competence, and adaptability to changing environments. The importance of mastering these skillsets has steadily increased in importance over the past two decades and continues to be an underdeveloped area of many veterinary curricula. There are two manuscripts included in this dissertation. Manuscript 1 (Chapter 2) is a literature review exploring the development of professional competency teaching over the past 20 years and its inclusion into the curriculum at most veterinary colleges. The first manuscript concludes by describing recommendations from the literature for effective methods of inclusion of professional competencies into the veterinary curriculum. Manuscript 2 (Chapter 3) details a study conducted in a veterinary college in which students were surveyed and asked about multiple aspects of their veterinary courses. The purpose of this study was to identify teaching strategies that can be used to improve the quality of teaching in professional competency courses, with the hope of improving students' motivation and effort levels as well. Together, these manuscripts contribute to current motivational theories and offer instructional design ideas to curriculum designers and educators who wish to improve students' motivation and engagement in professional competency development.

In commercial settings, broiler chickens and rainbow trout are housed in barren environments under high stocking densities, due to an emphasis on production efficiency. These monotonous housing conditions do not provide broilers or trout with the ability to perform functional, highly-motivated behaviors and increase their susceptibility to excessive anxiety and fear, resulting in negative affective states and poor animal welfare. Affective state (or emotional state) is a cumulative product of short-term life experiences, ranging from positive to negative. Because affective states are largely influenced by environmental condition, determining animal affective state can provide useful information on how to improve housing conditions in order to ensure positive experiences and good animal welfare. Cognitive processes are closely associated with affective state; a "cognitive bias" occurs when affective state influences aspects of cognition, such as judgement and attention. Animals in positive affective states make optimistically-biased decisions during ambiguous situations, judging the situation as if it will produce a positive outcome, and show less bias towards a perceived threat, responding in a less anxious and calm manner. Animals in negative affective states make pessimistically-biased decisions during ambiguous situations, judging the situation as if it will result in a negative outcome. Additionally, animals in negative affective states will bias their attention towards a perceived threat rather than alternative stimuli, responding in an anxious manner. Therefore, judgement and attention bias tests can be used to determine animal affective states. In Chapter 3, a judgement bias test was used to determine affective state of broiler chickens housed in either complex (perches, dust bath, pecking stones, and rotating enrichment objects) or barren (no enrichment) environments under either high or low stocking densities. Broilers housed in complex environments responded more optimistically during the judgement bias test than broilers from barren environments, indicating the former were in a positive affective state. Stocking density did not impact their responses in the judgement bias test, indicating that affective states were not impacted by that treatment. In Chapter 4, an attention bias test was used to determine level of anxiety and a tonic immobility test was used to determine fear in order to investigate affective state of broilers housed in the same conditions as described for Chapter 3. Broilers housed in complex environments were less anxious during the attention bias test than broilers from barren environments, indicating environmental complexity reduced anxiety in broilers. Stocking density did not impact anxiety. Broilers from high stocking density environments had shorter tonic immobility durations than broilers from low stocking density environments, suggesting the former were less fearful. Environmental complexity did not impact fearfulness. In Chapter 5, a judgement bias test was used to determine affective state of rainbow trout housed in either complex (shelter structure and artificial plants) or barren (no enrichment) tanks under either low or high stocking densities. Trout housed in high stocking density tanks responded optimistically during the judgement bias test, indicating they were in a more positive affective state compared to trout housed in low stocking density tanks. Environmental complexity did not impact their responses in the judgement bias test, indicating no effect of enrichments on affective states was found. These results indicate a beneficial relationship of a complex environment on broiler chicken affective state, observed through an optimistic judgement bias and reduced attention bias (anxiety) towards a perceived threat. Thus, providing a complex housing environment for broilers can improve their welfare and result in a positive affective state. Rainbow trout reared at the tested high density resulted in a positive affective state, although complexity did not benefit their welfare. Our results contribute much needed information on stocking densities to ensure fish welfare. Overall, environmental complexity, not stocking density, had a positive impact on broiler chicken affective states. Rainbow trout affective states were positively impacted by stocking density, but not environmental complexity. / Master of Science / Conventional housing of broiler chickens and rainbow trout (both raised for meat) causes concern for their welfare and affective states. Environmental conditions can greatly impact animals' affective states–their long-term emotional state, ranging from positive to negative. In barren environments at high stocking densities, broiler chickens and rainbow trout are prevented from showing normal behaviors and these conditions can compromise their affective state and welfare. By 'asking' chickens and trout whether the glass is half full or half empty, we can determine level of optimism or pessimism, and level of anxiety or calmness, therefore gaining a better understanding of their affective states. This can be done using a judgement bias test and attention bias test, where animal responses (optimism and anxiety) are recorded during ambiguous situations (judgement) and threatening situations (attention). Animals in positive affective states judge ambiguous situations optimistically (glass half full) and pay little attention towards perceived threats, while animals in negative affective states judge the same ambiguous situations pessimistically (glass half empty) and pay more attention towards perceived threats. In Chapter 3, responses to ambiguous situations were used to determine the affective state of broiler chickens housed in either enriched (perches, dust bath, pecking stones, rotating toys) or barren environments at either high or low stocking densities. Broiler chickens housed in enriched environments had an optimistic judgement bias of ambiguous situations (glass half full), suggesting they were in a more positive affective state compared to broilers housed in barren environments. Stocking density did not impact their level of optimism. In Chapter 4, responses to a perceived threat were used to determine level of anxiety and a tonic immobility test was used to determine fear of broilers housed under the same conditions as in Chapter 3. Broilers housed in enriched environments paid less attention to a perceived threat than broilers housed in barren environments, indicating the former were less anxious (glass half full) and in a positive affective state. Fear was not impacted by the tested enrichments, but birds kept under higher stocking densities did show reduced fear compared to birds in low-density environments. In Chapter 5, rainbow trout were housed in either enriched (shelter structure and artificial plants) or barren tanks at either high or low stocking densities. Affective state was evaluated through their responses to ambiguous situations. Trout housed in high stocking density environments had an optimistic judgement bias of ambiguous situations (glass half full), suggesting they were in a more positive affective state than trout housed in low stocking density environments. The enrichments did not impact their responses during the test, suggesting they did not impact fish optimism. These results indicate that an enriched environment improves broiler affective state and welfare compared to conventional housing conditions, the tested densities did not impact their welfare. Although an enriched environment did not positively impact responses of trout during ambiguous situations, our results show that housing rainbow trout in large groups results in a positive affective state and improved welfare status compared to housing trout in small groups. Overall, environmental enrichment, not stocking density, had a positive impact on broiler chicken affective states. Rainbow trout affective states were positively impacted by stocking density, but not environmental enrichment.

Background: The effect of animal-assisted activities (AAA) on the animal participants has been minimally investigated and the welfare of these animals has been questioned. Cortisol, in conjunction with stress-associated behavior, has been utilized as an objective assessment of animal welfare. Objective: Salivary cortisol and behavior in AAA dogs were measured to test the null hypothesis that salivary cortisol concentration and behavior are not different in an AAA environment compared to home or neutral environments.  Hair cortisol was measured in AAA dogs to test the null hypothesis that there is no relationship between hair cortisol and salivary cortisol. Methods:  Fifteen healthy adult dogs registered with an AAA organization were recruited.  A hair sample was collected from each dog upon enrollment.  Saliva samples were collected from each dog every 30 minutes, starting 30 minutes prior to and 30 minutes after a standardized 60 minute session across 3 settings: an AAA session (AS) for college students in the communal area of a residence hall; a neutral session (NS) located in a novel room without interaction with a stranger; and a home session (HS).  Each session was videotaped continuously and behaviors were coded at three separate 5-minute intervals while the dog was petted by a stranger in the AS or handler in the NS and HS. Results:  Salivary cortisol levels were not different in the AS compared to HS, but were significantly higher in the NS compared to AS and HS.  Dogs exhibited significantly more standing and ambulating behavior in the AS compared to HS.  Salivary cortisol level was negatively correlated with panting and standing at specific time points in the NS and AS, respectively.  Hair cortisol level did not correlate with salivary cortisol level at any time point in any of the settings. Conclusions:  During a 60 minute AAA session, salivary cortisol concentration and stress-associated behavior were not different compared to when dogs spent the same amount of time in the home setting, suggesting that they were not stressed when being used as AAA animals. The physical environment may be an important consideration when evaluating the effect of AAA on dogs.  Hair cortisol did not correlate with salivary cortisol, suggesting that hair may not be a representative predictor of cortisol in these environments.  Additional investigation is required to support cortisol and behavior as measures of stress and welfare in AAA animals. / Master of Science

Uruguay es el séptimo país exportador de carne vacuna del mundo y la producción agropecuaria se realiza mayoritariamente en base a pasturas naturales. Los elevados precios internacionales de la carne y la apertura de nuevos mercados en los últimos años, han provocado la intensificación de los sistemas de engorde. Hoy conviven los sistemas pastoriles tradicionales con los de engorde a corral, pasando por una serie de sistemas intermedios con distinto grado de intensificación. El desafío es mejorar la eficiencia del proceso de producción y la calidad del producto, sin afectar las peculiares características de los sistemas extensivos (bajo costo de producción, calidad nutricional de la carne) y sin comprometer el Bienestar animal y el medio ambiente. Es así que el presente trabajo de tesis pretende caracterizar estas diferentes alternativas de intensificación en lo que se refiere a Bienestar animal y calidad de carne, dado el creciente impacto de ambas temáticas en los precios del producto y en las preferencias de los consumidores de los países destino de las exportaciones. Para ello, se realizaron 2 experimentos en Uruguay durante los años 2005-2006 y 2006-2007, respectivamente. En el primer trabajo se utilizaron 84 novillos de raza Británica asignados a cuatro dietas con niveles incrementales de grano, desde una pradera artificial sin uso de suplemento, hasta un tratamiento de alimentación a corral en base a concentrado y heno. La ganancia de peso de los animales aumentó con el nivel energético de la dieta determinando diferentes fechas de faena para todos los tratamientos. Animales de temperamento más calmo tuvieron mayores ganancias de peso independientemente del nivel de alimentación. Los animales confinados mostraron una mayor dificultad para habituarse a las condiciones de producción, según indicadores fisiológicos relativos al estrés. Dichas dificultades pueden atribuirse en parte a la privación de realizar comportamientos probablemente relevantes para / Campo Gigena, MD. (2009). El bienestar animal y la calidad de carne de novillos en Uruguay con diferentes sistemas de terminación y manejo previo a la faena [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/4326

The purpose of this study is to give a deeper understanding of Anna Sewell’s argumentation for a changed view on animals in her book Black Beauty and Sewell’s contribution to the animal welfare debate. The questions addressed in the study is: What arguments does Sewell present for a changed view on animals? What contemporary assumptions does Sewell challenge? Against what contemporary assumptions does her argument become effective? To answer these questions the material that are examined is Sewell’s book Black Beauty: His Grooms and Companions, the Autobiography of a Horse (1877), first published in England. The theoretical perspective is that the book’s influence on the debate only is effective through two factors which is Sewell’s argumentation and the contemporary conditions that affected her way of arguing. These two factors become effective through interaction. The method used is a rhetorical analysis because it takes hold on text and context. Prior research has found through their perspective that the horses in Sewell’s book represent humans. But this study argues that the horses in Sewell’s book represent horses and that the book argues for a change in the way human’s attitude towards animals. The analysis in this study shows that Sewell’s argumentation can be divided into four categories of the contemporary ethics and context’s: Christianity, socialism, critique off fashion and machine. The conclusion drawn from the study is that Sewells arguments for a changed view on animals is based on these four categories where she must argue with and against them.

There are numerous guidelines recommending that captive primates live in complex environments in which they have the opportunity to make choices and the ability to control aspects of the environment, despite the lack of quantitative evidence to suggest these qualities improve welfare. Complexity, choice and control (the ‘Three Cs’) are inter-related and therefore it is complicated to separate their effects. The main aim of this thesis was to examine how the ‘Three Cs’ affect welfare, using the common marmoset (Callithrix jacchus) as a model. Behavioural measures and preference tests were used to determine the impact and significance of the ‘Three Cs’ on welfare. Experimental manipulations were natural (i.e. access to outside runs), or unnatural (e.g. pressing a button to control additional illumination). In a series of different studies, marmosets were moved to larger and more complex enclosures, were allowed to choose between indoor cages and outdoor complex enclosures and were able to control additional white light or coloured lights in their home enclosures. The results of these studies show that appropriate levels of each of the ‘Three Cs’ had a positive influence on the welfare of the marmosets, especially on youngsters. Although having control over light, and increased illumination itself improved welfare, providing a choice of access to outside runs (which were more complex and allowed the marmosets greater control over their activities) resulted in the greatest welfare improvement for marmosets of all ages. Loss of access, or control, did not appear to have a negative impact. The marmosets were housed in pairs or in family groups, in the different studies. A cross-study comparison shows that the composition of the groups affected the behavioural response of adult marmosets to environmental enrichment. Unexpectedly, it was also found that, when housed in standard laboratory conditions, adult marmosets were more relaxed when housed in pairs than when housed with their offspring. A secondary aim of the thesis was to quantify welfare indicators and activity budgets of common marmosets in a range of different social and physical contexts, and to compare this with the behaviour of wild marmosets, to increase our understanding of what is “normal” in captive situations. It is concluded that it is critical to sub-divide locomotion and inactivity into different levels to interpret these measures accurately. Levels of calm locomotion increased in enriched environments, while levels of relaxed inactivity and scent marking decreased. A number of recommendations for the care and housing of marmosets are made.

Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2018-08-21T17:41:05Z No. of bitstreams: 1 YURI FERNANDES LIMA.pdf: 1867812 bytes, checksum: 5e8fcfb25da2a9722de73dcc867767b9 (MD5) / Approved for entry into archive by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2018-08-21T17:42:22Z (GMT) No. of bitstreams: 1 YURI FERNANDES LIMA.pdf: 1867812 bytes, checksum: 5e8fcfb25da2a9722de73dcc867767b9 (MD5) / Made available in DSpace on 2018-08-21T17:42:22Z (GMT). No. of bitstreams: 1 YURI FERNANDES LIMA.pdf: 1867812 bytes, checksum: 5e8fcfb25da2a9722de73dcc867767b9 (MD5) / A presente dissertação questiona como garantir a efetividade da legislação internacional e nacional que veda práticas cruéis e maus-tratos às galinhas poedeiras na indústria de ovos. Dessa forma, no primeiro capítulo faço uma abordagem histórica sobre a exploração dos animais não humanos pelos humanos desde o advento da agricultura até os tempos atuais, passando pela domesticação das galinhas e o seu confinamento nas chamadas gaiolas em baterias. Analiso, ainda, os conceitos de crueldade, maus-tratos e bem-estar animal, bem como apresento alguns sistemas alternativos de criação de galinhas poedeiras. No segundo capítulo discorro sobre a proteção jurídica das galinhas poedeiras, tanto do ponto de vista conceitual quanto do ponto de vista legislativo, em âmbitos internacional e nacional. Analiso o movimento animalista, sobretudo nos dias atuais no que se refere à abolição das gaiolas em baterias. No terceiro capítulo apresento a certificação de bem-estar como solução possível ao problema inicialmente apresentado. Para isso, disserto sobre a natureza jurídica das certificações e a sua importância para a garantia do direito de informação do consumidor, discorro sobre os principais selos verdes e de proteção animal existentes no Brasil e analiso pormenorizadamente a certificação de bem-estar animal aplicada às galinhas poedeiras para, ao fim, abordar a questão da consciência do consumidor brasileiro sobre o tema. Concluo defendendo que (i) a certificação de bem-estar seja obrigatória; (ii) sejam utilizados critérios técnicos para aferir o grau de bem-estar e, consequentemente, a ocorrência ou não de maus-tratos, bem como que tais critérios sejam estabelecidos em lei; (iii) sejam estabelecidos por lei os critérios para que o INMETRO acredite uma certificadora; (iv) a fiscalização da certificação seja feita por agência reguladora; (v) seja instituída uma política informativa; e (vi) seja franqueado o acesso da população às granjas. Isso, por um lado, obrigará os produtores a adequarem-se às normas mínimas de bem-estar das galinhas poedeiras, sob pena de serem responsabilizados criminalmente, e, por outro lado, possibilitará que o consumidor faça escolhas conscientes, boicotando os produtores que insistirem em maus-tratos, o que os fará desaparecer, e estimulando os produtores que observarem o bem-estar, que se proliferarão. / The present dissertation questions how to ensure the effectiveness of international and national legislation that prohibits cruel practices and mistreatment of laying hens in the egg industry. Thus, in the first chapter I make a historical approach to the exploitation of nonhuman animals by humans from the advent of agriculture to the present, through the domestication of chickens and their confinement in so-called battery cages. I also analyze the concepts of cruelty, maltreatment and animal welfare, as well as present some alternative systems for laying hens. In the second chapter I discuss the legal protection of laying hens, both conceptually and from a legislative point of view, at international and national levels. I analyze the animalistic movement, especially in the present day regarding the abolition of battery cages. In the third chapter I present the animal welfare certification as a possible solution to the problem initially presented. To do this, I speak about the legal nature of certifications and their importance for guaranteeing the right of information to the consumer, I write about the main green and animal protection stamps in Brazil, analyzing in detail the certification of applied animal welfare to laying hens, in order to address the issue of Brazilian consumer awareness on the subject. I conclude defending that (i) welfare certification must be mandatory; (ii) technical criteria must be used to assess the degree of well-being and, consequently, the occurrence or non-existence of maltreatment, and that such criteria must be established by law; (iii) the criteria for INMETRO to accredit a certifier must be established by law; (iv) certification inspection must be done by a regulatory agency; (v) an information policy must be instituted; and (vi) the access of the population to the farms must be allowed. This, on the one hand, will oblige producers to comply with the minimum standards of welfare of laying hens, otherwise they will be criminally liable, and on the other hand, it will enable the consumer to make conscious choices by boycotting producers who insist in maltreatment, which will make them disappear, and stimulating producers who observe the well-being, which will proliferate.

Orientador: Ronaldo Romulo Machado de Almeida / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Filosofia e Ciências Humanas / Made available in DSpace on 2018-08-18T11:19:48Z (GMT). No. of bitstreams: 1 Ramiro_DanielPereira_M.pdf: 717945 bytes, checksum: 643d85ffbfc1affda0148033567c4c2e (MD5) Previous issue date: 2011 / Resumo: A presente dissertação busca rastrear o contradiscurso acerca da experimentação animal no sentido de elucidá-lo enquanto um movimento social. Toma como foco o trâmite da Lei Arouca, de recente aprovação no Congresso Nacional para regulamentar a vivissecção em todo território nacional. A partir desta lei o olhar se volta aos atores sociais envolvidos e às forças políticas conflitantes para esta questão de controvérsia científica. Paralelamente, outras manifestações práticas do contradiscurso serão seguidas a fim de enriquecer o material de análise para colocar em evidência os argumentos que concorrem para a construção simbólica do estatuto do animal de laboratório. Porém, não será perdido de vista o caráter mais amplo no qual o movimento antivivisseccionista está inserido, a saber, a luta contra a exploração dos animais não-humanos / Abstract: This dissertation intends to follow the counter-discourse on animal experimentation in order to elucidate it as a social movement. It is focused on the processing of Arouca Law, recently passed the Congress to regulate vivisection nationwide. From this law, the focus is on the social actors involved and the political conflicting forces for this matter of scientific controversy. In parallel, other practical manifestations of counter-discourse will be followed in order to enrich the material for analysis to highlight the arguments that contribute to the symbolic status of laboratory animals. However, the broader nature in which the antivivisection movement is inserted, namely the fight against the exploitation of nonhuman animals, will not be lost / Mestrado / Antropologia Social / Mestre em Antropologia Social

In the UK, laboratory-housed dogs are primarily used as a non-rodent species in the safety testing of new medicines and other chemical entities. The use of animals in research is governed by the Animals (Scientific Procedures) Act (1986, amended 2012) and legislation is underpinned by the principles of humane experimental technique: Replacement, Reduction and Refinement. A link between animal welfare and the quality of data produced has been shown in other species (e.g. rodents, nonhuman primates), however, no established, integrated methodology for identifying or monitoring welfare and quality of data output previously existed in the laboratory-housed dog. In order to investigate the effects of planned Refinements to various aspects of husbandry and regulated procedures, this project sought to integrate behavioural, physiological and other measures (e.g. cognitive bias, mechanical pressure threshold) and to provide a means for staff to monitor welfare whilst also establishing the relationship between welfare and quality of data output. Affective state was identified using an established method of cognitive bias testing, before measuring welfare at ‘baseline’ using measures of behaviour and physiology. Dogs then underwent ‘positive’ and ‘negative’ behavioural challenges to identify the measures most sensitive to changing welfare and most suitable for use in a framework. The resulting Welfare Assessment Framework, developed in three groups of dogs from contrasting backgrounds within the facility, found a consistent pattern of behaviour, cardiovascular function, affect and mechanical pressure threshold (MPT). Dogs with a negative affective state had higher blood pressure at baseline than those with positive affective states, and the magnitude of the effect of negative welfare suggests that welfare may act as a confound in the interpretation of cardiovascular data. The responses to restraint included increases in blood pressure and heart rate measures which approached ceiling levels, potentially reducing the sensitivity of measurement. If maintained over time this response could potentially have a negative health impact on other organ systems and affecting the data obtained from those. Dogs with a negative welfare state also had a lower mechanical pressure threshold, meaning they potentially experienced greater stimulation from unpleasant physical stimuli. Taken together with the behaviours associated with a negative welfare state (predominantly vigilant or stereotypic behaviours) the data suggest that dogs with a negative welfare state have a greater behavioural and physiological response to stimuli in their environment; as such, data obtained from their use is different from that obtained from dogs with a positive welfare state. This was confirmed by examining the effect size (Cohen’s d ) resulting from the analysis of affective state on cardiovascular data. An increase in variance, particularly in the small dog numbers typical of safety assessment studies, means a reduction in the power of the study to detect the effect under observation; a decrease in variation has the potential to reduce the number of dogs use, in line with the principle of Reduction and good scientific practice. The development of the framework also identified areas of the laboratory environment suitable for Refinement (e.g. restriction to single-housing and restraint) and other easily-implemented Refinements (e.g. feeding toy and human interaction) which could be used to improve welfare. As a result of this, a Welfare Monitoring Tool (WMT) in the form of a tick sheet was developed for technical and scientific staff to identify those dogs at risk of reduced welfare and producing poor quality data, as well as to monitor the effects of Refinements to protocols. Oral gavage is a common regulated procedure, known to be potentially aversive and was identified as an area in need of Refinement. A program of desensitisation and positive reinforcement training was implemented in a study also comparing the effects of a sham dose condition versus a control, no-training, condition. A number of the measures used, including home pen behaviour, behaviour during dosing, MPT and the WMT showed significant benefits to the dogs in the Refined condition. Conversely, dogs in the sham dose condition showed more signs of distress and took longer to dose than dogs in the control condition. The welfare of control dogs was intermediate to sham dose and Refined protocol dogs. This project identified a positive relationship between positive welfare and higher quality of data output. It developed and validated a practical and feasible means of measuring welfare in the laboratory environment in the Welfare Assessment Framework, identified areas in need of Refinement and developed practical ways to implement such Refinements to husbandry and regulated procedures. As such it should have wide implications for the pharmaceutical industry and other users of dogs in scientific research.