Made available in DSpace on 2014-06-11T19:24:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2003Bitstream added on 2014-06-13T20:30:50Z : No. of bitstreams: 1 santin_f_me_botfmvz.pdf: 219523 bytes, checksum: b72d17321430b61e30a3324a044f0c1c (MD5) / A presença de uma função renal adequada é importante para a manutenção da homeostase. A anfotericina b é um antifúngico potente, cuja utilização tem sido limitada devido ao seu elevado potencial nefrotóxico. O presente trabalho teve por objetivos acompanhar, laboratorialmente, a função renal de cães saudáveis submetidos à administração de doses terapêuticas do antifúngico anfotericina b (FungisonÒ), bem como avaliar dentre os métodos laboratoriais empregados, qual o método mais sensível e precoce de diagnóstico de lesão renal. A função renal foi avaliada por meio da realização diária de urinálise, da excreção fracionada dos eletrólitos sódio e potássio, dosagem das concentrações séricas de creatinina e uréia e atividade da enzima gama-glutamiltransferase (GGT) urinária. Cada animal foi seu próprio controle, uma vez que foram obtidas amostras de urina e sangue de cada animal, para realização dos exames acima citados, antes da primeira aplicação do fármaco. Concluiu-se que a anfotericina b provoca lesões tanto em túbulo proximal quanto em distal, onde a alteração em túbulo distal foi a primeira a ser detectada, por meio da diminuição dos valores de densidade urinária; dosagem de creatinina e uréia séricas e potássio sérico, deve ser realizada antes de cada aplicação do fármaco; a enzima urinária gama-glutamiltrasferase (GGT) não foi eficaz como método de diagnóstico de lesão renal induzida por anfotericina b; demonstrou-se também que a anfotericina b provoca acidose tubular renal do tipo I e induz diabetes insipidus nefrogênico em cães. / Anfotericina B is a strong anti-fungus agent, which use has been limited due to its high nephrotoxic potential. The objective of this experiment is to accompany in the laboratory the renal function of healthy dogs which were submitted to therapeutic doses of Anfotericina B (Fungison®), as well as to evaluate which method can first diagnose kidney lesions with the most precise results. Ten healthy adult male dogs were used in the experiment. All dogs received five doses of 0.5 mg/kg of Anfotericina B every other day. The renal function was evaluated through daily urine exams, fractioned excretion of the electrolytes sodium and potassium, doses of serical concentrations of creatine and urea and the activity of the enzyme gama-glutamiltransferase (GGT) [urinaria]. Throughout the experiment, each animal was individually tested. We concluded that Anfoteracina B provokes lesions both in the proximal and distal tubes. Urinalysis proved to diagnose kidney lesions in its earliest stage through a modification of the density parameters. The dosage of creatine and serical urea must be effected before each application of Anfoteracina B. Finally, urinal enzyme gama-glutamiltrasferase (GGT) should not be used as a method to diagnose kidney lesions caused by therapeutic doses of Anfotericina B. The experiment also demonstrates the importance of accompanying the serical concentration of potassium during the treatment with Anfotericina B. Finally, we concluded that Anfotericina B resulted in tubular acidosis type I of the kidney, as well as induces nephrogenic diabetes insipidus in dogs.

Made available in DSpace on 2016-07-01T13:10:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-29. Added 1 bitstream(s) on 2016-07-01T13:13:46Z : No. of bitstreams: 1 000866649.pdf: 1623813 bytes, checksum: 7a365c7b113a6cb2107156da7f33dbc3 (MD5) / A dermatite atópica é uma doença de pele alérgica, inflamatória e pruriginosa que acomete cães geneticamente predispostos. A característica mais comum é o prurido que exerce impacto significativo na qualidade de vida do animal bem como do seu proprietário. A interleucina-31 (IL-31) é uma citocina envolvida com o grau de prurido nas alergopatias, enquanto a filagrina (FLG) é uma proteína relacionada com a adequada queratinização e hidratação do estrato córneo. O intuito desse estudo foi padronizar e quantificar a técnica de imunohistoquímica (IHQ) para IL-31 e filagrina e associa-las ao grau de prurido e restauração de barreira cutânea na dermatite atópica antes e após o tratamento tópico com repositor de lipídeos na epiderme. Todos os cães foram provenientes do atendimento clínico do Hospital Veterinário da FMVZ- Unesp Botucatu-SP. Sobre a IL-31 não houve diferença significativa entre sua expressão quando comparadas as regiões dorsal e axilar de cães normais. Quando comparamos a expressão da IL-31 entre cães com dermatite atópica e saudáveis foi possível notar maior expressão de IL-31 em cães com dermatite atópica. Quando comparamos os cães com dermatite atópica antes e após aplicação do repositor de lipídeos na epiderme a expressão da IL-31 diminuiu significativamente após a aplicação. Em se tratando da proteína filagrina não foi encontrada diferença significativa entre sua expressão quando comparadas a região axilar e cervical de cães saudáveis, o mesmo ocorreu entre essas regiões em cães com dermatite atópica antes do tratamento e após o tratamento, quando comparados de maneira isolada. Quando comparamos as regiões axilar e cervical dos cães com dermatite atópica antes e após o tratamento não houve o aumento da expressão da proteína filagrina como o esperado. Houve diferença significativa entre as regiões cervical e axilar de cães com dermatite atópica quando comparados... / Atopic dermatitis is an allergic, inflammatory and pruritic skin disease affecting genetically predisposed dogs. The most common feature is cutaneous rash that significantly impacts the animals' and owners' quality of life. Interleukin-31 (IL-31) is a cytokine involved in the degree of itching in allergies, while filaggrin (FLG) is a protein associated with adequate hydration and keratinization of the stratum corneum. The purposes of this study were to standardize the technique of immunohistochemistry (IHC) for IL-31 and filaggrin and to quantify and associate them to the degree of itching and skin barrier recovery in dogs with atopic dermatitis before and after topical treatment for lipids restitution in the epidermis. All dogs came from the clinical care of the Veterinary Hospital of FMVZ- Unesp Botucatu. There was no significant difference on the expression of IL-31 between samples obtained either from the dorsal or axillary regions of healthy dogs. When atopic dermatitis and healthy animals were compared, expression of IL-31 was noticeable higher in atopic dermatitis animals. IL-31 quantification in the atopic dermatitis animals evidenced significantly lower expression after the use of skin lipids complex in the epidermis. In the case of filaggrin protein, there was no significant difference between its expression when the axillary and cervical sites were compared both in healthy dogs and atopic dermatitis dogs and also when atopic dermatitis dogs were compared before and after treatment. Nevertheless significant difference in fillagrin levels was observed between the cervical and axillary regions of atopic dermatitis dogs when compared to healthy dogs; filagrina levels in healthy animals were larger than in atopic dermatitis dogs. IL-31 results suggest that this cytokine is involved in the physiology of atopic pruritus and that restitution of the skin epidermal barrier is essential so that we can control the pruritus and immunological...

Made available in DSpace on 2016-09-27T13:39:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-04. Added 1 bitstream(s) on 2016-09-27T13:45:08Z : No. of bitstreams: 1 000870645_20170104.pdf: 242400 bytes, checksum: 9d69e8bb5f77471c03c024ed5ca56917 (MD5) Bitstreams deleted on 2017-01-06T13:21:19Z: 000870645_20170104.pdf,. Added 1 bitstream(s) on 2017-01-06T13:22:02Z : No. of bitstreams: 1 000870645.pdf: 525092 bytes, checksum: 20064957cf347be120a7f3cbbb1f7137 (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Dogs infected with Leishmania infantum showed a reduction in the number of lymphocytes T. PD-1 (Programmed cell death 1) a new member of the B7-CD28 family, it is expressed by cells of the immune system and its binding to PD-L1 (CD274) or PD-L2 (CD273) induces deactivation of T cells or apoptosis. This study aimed to evaluate the PD-1 expression and its ligands as well as their role in T lymphocyte induction of apoptosis, TNF-α and IL-4 secretion, nitric oxide production and parasite load in dogs with visceral leishmaniasis. We observed that in canine visceral leishmaniasis PD-1 and its ligands involved in induction of apoptosis of T lymphocytes, are regulating the nitric oxide production, TNF-α, IL-4, as well as the parasitic load. This study helps to clarify the mechanism of immune response and immunotherapeutic drugs such as blocking monoclonal antibodies that can influence the LVC / FAPESP: 2013/066849

Made available in DSpace on 2016-09-27T13:40:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01. Added 1 bitstream(s) on 2016-09-27T13:45:09Z : No. of bitstreams: 1 000868779.pdf: 1054511 bytes, checksum: 839f416844e6ec28b8daeb9e6fd8b61c (MD5) / Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) / Ehrlichiose Monocítica Canina (EMC) é uma enfermidade infecciosa, potencialmente fatal, causada por Ehrlichia canis, transmitida pelo carrapato Rhipicephalus sanguineus. O curso da enfermidade é dividido em três fases: aguda, subclínica e crônica. O objetivo deste estudo foi avaliar as lesões anatomopatológicas que ocorreram no parênquima e no endotélio vascular do encéfalo, coração, pulmão, fígado, rim, baço e linfonodo mesentérico de 16 cães que vieram a óbito acometidos pela EMC na fase crônica. Esses animais apresentaram sinais clínicos neurológicos, porém na avaliação microscópica não exibiam lesões que justificassem esses sintomas. No coração foi possível notar a presença de alterações vasculares visíveis, como Coagulação Intravascular Disseminada, necrose dos miócitos próximos a esses vasos, congestão e hemorragias devido à severa trombocitopenia desenvolvida durante a infecção. No pulmão notou-se pneumonia em grande parte dos animais estudados. O fígado também se apresentou acometido, mas não diretamente pela infecção, mas sim por consequência de outros fatores prejudiciais relacionados à doença. Em todos os rins avaliados a glomerulonefrite se mostrou presente. Em todos os 16 animais pode-se notar a esplenomegalia. O linfonodo mesentérico não é uma estrutura muito estudada em animais com EMC, mas pode-se notar um grande infiltrado plasmocitário. No entanto, cocluiu-se que a vasculite foi responsável por grande parte das lesões, sendo predominante o infiltrado plasmocitário / Canine Monocytic Ehrlichiosis (CME) is an infectious disease, potentially fatal, caused by Ehrlichia canis, transmitted by Rhipicephalus sanguineus. The course of the disease is divided into three stages: acute, chronic and subclinical. The aim of this study was to evaluate the pathological lesions that occurred in the parenchyma and the vascular endothelium of the brain, heart, lung, liver, kidney, spleen and mesenteric lymph node of 16 dogs that died affected by CME in the chronic phase. These animals showed neurological signs, but in microscopic evaluation showed no injury to justify these symptoms. In the heart it was possible to notice the presence of visible vascular abnormalities, such as disseminated intravascular coagulation, necrosis of the myocytes near these vessels, congestion and bleeding due to severe thrombocytopenia developed during infection. In the lung was noted pneumonia in most animals studied. The liver also appeared affected, but not directly by infection, but as a result of other harmful factors related to the disease. In all evaluated kidneys showed this glomerulonephritis. In all 16 animals can be noted splenomegaly. The mesenteric lymph node is not a very studied structure in animal CME, but you may notice a large plasma cell infiltrate. However, the vasculitis was responsible for much of the lesions, predominant being the plasma cell infiltrate

Made available in DSpace on 2016-09-27T13:40:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-21. Added 1 bitstream(s) on 2016-09-27T13:45:11Z : No. of bitstreams: 1 000870504.pdf: 2094890 bytes, checksum: 4efcd5daececceda5488d900500bc29b (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The study aimed to evaluate the effects of administration of ropivacaine 0.75% combined with dexmedetomidine on the sciatic and femoral nerves in dogs. Seven adult and healthy Beagle dogs were submitted to three experimental groups. In GCON, animals received perineural ropivacaine 0,75% (0.1mL/kg/nerve); GDPN received perineural dexmedetomidine diluted (1μg/mL) in ropivacaine 0,75% (0.1mL/kg/nerve); and GDIM received perineural ropivacaine 0,75% (0.1mL/kg/nerve) and dexmedetomidine diluted in saline (1μg/mL) via intramuscular administration (0,2mL/kg). The perineural administrations were carried out under general inhalation anesthesia and guided by ultrasound and nerve stimulation. Onset time and duration of sensory and motor blocks were evaluated, as well as patellar, gastrocnemius and cranial tibial reflexes, heart (FC) and respiratory rate (f), systolic arterial pressure (PAS), rectal temperature (TR), lactate (LACT), blood glucose (GLIC), sedation (SED) and blood gas analysis during 240 minutes. No differences were observed between groups with regards to FC, f, PAS, LACT, GLIC, TR, SED, blood gas, reflexes and sensory and motor block latency. However, the duration of the tibial sensory block was significantly longer in GDPN compared with GDIM (p=0,0302). Administration of perineural or intramuscular dexmedetomidine did not cause changes in physiologic parameters or the onset time and duration of motor block. The perineural administration of dexmedetomidine prolonged the duration of the sensory block only for the tibial nerve.

Made available in DSpace on 2016-09-27T13:40:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-07-30. Added 1 bitstream(s) on 2016-09-27T13:45:12Z : No. of bitstreams: 1 000870581.pdf: 733749 bytes, checksum: 3578956d53951ef3b36e5b1d6580f155 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Periodontal disease (PD) is the most common disease of the oral cavity in dogs is inflammatory, chronic and infectious and is responsible for the production of reactive species of oxygen (ROS). Excessive ROS can lead to the situation known as oxidative stress, or imbalance of oxidants and antioxidants. Systematic review was performed on PD and oxidative stress to investigate the hypothesis that the canine PD suffers oxidative stress and this varies with the degree of the disease. To this end, we selected 22 dogs with PD, all adults, of different races and genders. The control group was made up of the same dogs, 30 days after periodontal treatment, all without any changes in physical and laboratorial examination. To evaluate oxidative stress was measured the full blood antioxidant capacity (TAC), plasma antioxidant, the total concentration of plasma oxidant (OCD), oxidative stress index, oxidative metabolism of circulating neutrophils (superoxide production by cytochemical test of reduction of nitroblue tetrazolium - NBT) and plasma lipid peroxidation (concentration of thiobarbituric reactive species - TBARS). Studies linking oxidative stress and PD are still rare in dogs, and humans with periodontitis has more oxidative stress compared to the control group. Oxidative stress in dogs PD was confirmed in peridontites (TAC decrease and increased neutrophil superoxide production), but these changes did not differ as to the degree of injury and were not significant in gingivitis. This is probably the first evidence that systemic oxidative stress occurs in canine periodontitis and that neutrophils

Made available in DSpace on 2016-09-27T13:40:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-23. Added 1 bitstream(s) on 2016-09-27T13:45:12Z : No. of bitstreams: 1 000870463.pdf: 877740 bytes, checksum: 79b4a09d0e6fd75bfc905a39a77c9f0d (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Intracameral injection is used for treatment and anesthesia of the eye bulb and opioids are known to provide analgesia through this route. The aim of the study was to evaluate the viability and efficacy of intracameral nalbuphine and morphine in dogs. Seven beagle dogs were randomly included in the groups of intracameral nalbuphine (GNIC), intracameral morphine (GMIC), intravenous nalbuphine (GNIV) and intravenous morphine (GMIV) injection of either opioid. Variables were heart rate, respiratory rate, systolic arterial pressure, subjective pain scale, Schirmer's test, pupillary diameter, intraocular pressure, fluorescein test, corneal touch threshold, number and type of local or systemic complications and duration of effect on the cornea. Time point comprised baseline (T0) and measurements every 30 minutes after drugs administration up to 240 minutes. Parametric variables were analyzed through ANOVA for repeated measures and Dunnett's test. Ordinal data were compared through Friedman's test, followed by Dunn's test. Analyses were performed by SAS and were considered significant when P<0.05. Analgesia lasted significantly longer in GNIC, whereas GMIC, GNIV and GMIV did not differ between one another. There was a significant decrease in the IOP at T30 in all groups when compared to baseline measures. As for complications, no difference was found between groups. Therefore, both nalbuphine and morphine can be used for intracameral analgesia, and nalbuphine is the preferred option due to the prolonged analgesia. / FAPESP: 2013/02162-4

Orientador: Mirela Tinucci Costa / Banca: Helio José Montassier / Banca: Silvia Regina Ricci Lucas / Banca: Felipe Augusto Ruiz Sueiro / Banca: Sabryna Gouveia Calazans / Resumo: O linfoma é a neoplasia hematopoiética mais comum nos cães e uma das mais frequentes, dentre todas as neoplasias, nesta espécie. Apresenta-se em diversas localizações anatômicas e pode apresentar classificações histológicas e imunofenotipos distintos. Dependendo da resposta imune do paciente frente à instalação de um tumor, algumas informações sobre o prognóstico podem ser obtidas. Atualmente, as células T reguladoras (Tregs) vêm sendo estudadas em algumas neoplasias caninas, por seu comprovado potencial imunossupressor, principalmente por inibir a resposta antitumoral. Neste contexto, o presente trabalho teve como objetivos quantificar, por citometria de fluxo, as células Tregs em sangue periférico de cães com linfoma multicêntrico de imunofenótipos B e T, respectivamente 14 e 8 cães, no momento do diagnóstico e após o primeiro ciclo de quimioterapia antineoplásica com o protocolo Madison-Wisconsin (MW) de 19 semanas adaptado, e comparar com cães saudáveis (n=10), buscando quantificá-las em cães com linfoma de diferentes imunofenótipos antes e após a 5ª semana do protocolo MW. Os resultados mostraram que cães com linfoma apresentaram uma porcentagem significativamente maior de Tregs (18,84±2,56) quando comparada aos cães sem neoplasia (4,70±0,50) (p<0,01). Além disso, após a 5ª semana de tratamento houve uma significante redução da população de Tregs (7,54±1,08), atingindo valores semelhantes a dos cães controle (4,70±0,50) (p>0,05). Não houve diferença nas Tregs em relação aos imunofenotipos B (17,45±2,77) e T (21,27±5,27) (p>0,05). Concluiu-se que o linfoma em cães leva a um aumento de células Tregs e que o tratamento com o protocolo quimioterápico MW reduz significativamente as células Tregs em sangue periférico, atingindo valores próximos aos dos cães saudáveis / Abstract: Lymphoma is the most common hematopoietic malignancy in dogs and one of the most frequent among all neoplastic diseases in this species. It can occur in several anatomical locations with distinct histological and immunophenotypes. Depending on the host immune response towards the tumor, prognosis information could be collected. Because its well established immunosuppressant, antitumor activity, the function of regulatory T cells (Tregs) in canine neoplasias has been investigated. In this study, we sought to quantify, using flow cytometry, the Tregs subpopulation in peripheral blood of healthy dogs (10) and in those diagnosed with type-B (14) and type-T (8) multicentric lymphoma before (at diagnosis) and after the first cycle (5-week) of 19-week Madison- Wisconsin (MW) protocol of chemotherapy. Our results indicated that dogs with lymphoma showed higher percentage of Tregs (18,84±2,56) when compared to healthy dogs (4,70±0,50) (p<0,01). In addition, 5-week chemotherapy treatment reduced the Tregs subpopulation (7,54±1,08) to levels similar to control (4,70±0,50) (p>0,05). There was no difference in Tregs percentage between B-type (17,45±2,77) and T-type (21,27±5,27) lymphoma (p>0,05). With this, we conclude that canine lymphoma increases Tregs in the peripheral blood and the MW protocol of chemotherapy reduces this cell subpopulation to control values / Doutor

Orientador: Áulus Cavalieri Carciofi / Banca: Áureo Evangelista Santana / Banca: Ricardo Souza Vasconcellos / Banca: Lilian Rose Marques de Sá / Banca: Márcio Antonio Brunetto / Resumo: As possíveis diferenças que acometem cães de diferentes idades ainda são pouco estudadas e descritas para esta espécie. O presente estudo avaliou e comparou a composição da microbiota nas fezes e seus produtos de fermentação, aspectos histológicos da mucosa intestinal e a imunidade de cães filhotes, adultos e velhos. Para tal, 30 cães beagle foram divididos igualmente em três grupos: o grupo filhotes (GF), composto por filhotes do desmame aos 10 meses de idade; o grupo adultos (GA), composto por cães adultos entre cinco e seis anos e o grupo velhos (GV), composto por cães velhos entre 10 e 13 anos. Os animais receberam a mesma dieta e nenhum outro tipo de intervenção por 30 dias e após este período amostras de fezes frescas foram colhidas para a mensuração de: pH, determinação da concentração de lactato, ácidos graxos de cadeia curta e ramificada, amônia, indol, fenol, aminas, imunoglobulina A e composição da microbiota. Também foi adquirida amostra de sangue periférico para quantificação das populações linfocitárias CD4+CD5+, CD5+, CD8+CD5+, e CD21+ por citometria de fluxo, sendo esta analisada em cinco idades (45, 66, 87, 105 e 300 dias) do GF e a média destas avaliações comparada com GA e GV. Após esta análise, foi realizado teste de hipersensibilidade cutânea tardia e ao final destes, os animais foram anestesiados e submetidos a procedimento endoscópico para coleta de fragmentos do estômago e intestinos delgado e grosso para análise histológica dos mesmos. Considerou-se como significativos valores de P≤0,05 e como tendência valores de P<0,1. Observou-se que o GF apresentou maior número de Bifidobacterium spp. e Cluster IV. GV apresentou indicativo de menor atividade sacarolítica bacteriana observada pela menor concentração de acetato, propionato, butirato e ácidos graxos voláteis totais o que... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: Possible differences that may occur in dogs of different ages have been little studied and described in this specie. The aim of this study was to evaluate and compare the composition of microbiota and some fermentation products in the feces, histological aspects of intestinal mucosa and immunity of puppies, adults and senior dogs. To perform this, 30 beagle dogs were divided into three groups: Puppy group (GF), composed of puppies from weaning to 10 months old, Adult group (GA), composed of adult dogs between five and six years and Old group (GV), composed of old dogs between 10 and 13 years. Animals received the same diet and no other intervention for 30 days, and after this period, fresh fecal samples were collected for measurement of pH, determining the concentration of lactate, short and branched chain fatty acids, ammonia, indol, phenols, amines, immunoglobulin A and composition of the microbiota. It was also acquired a peripheral blood sample for quantification of lymphocyte populations CD4+CD5+, CD5+, CD8+CD5+, and CD21+ by flow cytometry, which was analyzed in five ages of GF (45, 66, 87, 105 and 300 days) and the average of these evaluations compared with GA and GV. After this analysis was performed the delayed type hypersensitivity test and by the end of this, animals were anesthetized and submitted to an endoscopic procedure for collecting fragments of stomach and small and large intestines for histological analysis. P values ≤0.05 was considered significant and P values <0.1 as a trend. GF showed greater number of Bifidobacterium spp. and Cluster IV. GV presented an indication of reduced bacterial saccharolytic activity observed by the lower concentration of acetate, propionate, butyrate, and total volatile fatty acids which resulted in higher pH in the feces of this group. GF showed... (Complete abstract click electronic access below) / Doutor

Orientador : Flávio Quaresma Moutinho / Resumo: A presença de uma função renal adequada é importante para a manutenção da homeostase. A anfotericina b é um antifúngico potente, cuja utilização tem sido limitada devido ao seu elevado potencial nefrotóxico. O presente trabalho teve por objetivos acompanhar, laboratorialmente, a função renal de cães saudáveis submetidos à administração de doses terapêuticas do antifúngico anfotericina b (FungisonÒ), bem como avaliar dentre os métodos laboratoriais empregados, qual o método mais sensível e precoce de diagnóstico de lesão renal. A função renal foi avaliada por meio da realização diária de urinálise, da excreção fracionada dos eletrólitos sódio e potássio, dosagem das concentrações séricas de creatinina e uréia e atividade da enzima gama-glutamiltransferase (GGT) urinária. Cada animal foi seu próprio controle, uma vez que foram obtidas amostras de urina e sangue de cada animal, para realização dos exames acima citados, antes da primeira aplicação do fármaco. Concluiu-se que a anfotericina b provoca lesões tanto em túbulo proximal quanto em distal, onde a alteração em túbulo distal foi a primeira a ser detectada, por meio da diminuição dos valores de densidade urinária; dosagem de creatinina e uréia séricas e potássio sérico, deve ser realizada antes de cada aplicação do fármaco; a enzima urinária gama-glutamiltrasferase (GGT) não foi eficaz como método de diagnóstico de lesão renal induzida por anfotericina b; demonstrou-se também que a anfotericina b provoca acidose tubular renal do tipo I e induz diabetes insipidus nefrogênico em cães. / Abstract: Anfotericina B is a strong anti-fungus agent, which use has been limited due to its high nephrotoxic potential. The objective of this experiment is to accompany in the laboratory the renal function of healthy dogs which were submitted to therapeutic doses of Anfotericina B (Fungison®), as well as to evaluate which method can first diagnose kidney lesions with the most precise results. Ten healthy adult male dogs were used in the experiment. All dogs received five doses of 0.5 mg/kg of Anfotericina B every other day. The renal function was evaluated through daily urine exams, fractioned excretion of the electrolytes sodium and potassium, doses of serical concentrations of creatine and urea and the activity of the enzyme gama-glutamiltransferase (GGT) [urinaria]. Throughout the experiment, each animal was individually tested. We concluded that Anfoteracina B provokes lesions both in the proximal and distal tubes. Urinalysis proved to diagnose kidney lesions in its earliest stage through a modification of the density parameters. The dosage of creatine and serical urea must be effected before each application of Anfoteracina B. Finally, urinal enzyme gama-glutamiltrasferase (GGT) should not be used as a method to diagnose kidney lesions caused by therapeutic doses of Anfotericina B. The experiment also demonstrates the importance of accompanying the serical concentration of potassium during the treatment with Anfotericina B. Finally, we concluded that Anfotericina B resulted in tubular acidosis type I of the kidney, as well as induces nephrogenic diabetes insipidus in dogs. / Mestre