Orientador: Marcelo Vasconcelos Meireles / Banca:Juliana Peloi Vides / Banca:Heito Flávio Ferrari / Banca: Katia Denise Saraiva Bresciani / Banca:Cáris Maroni Nunes / Resumo: O presente trabalho objetivou a identificação e caracterização molecular de isolados de Cryptosporidium em amostras fecais de cães e gatos, bem como o desenvolvimento de uma reação em cadeia pela polimerase (PCR) em tempo real para detecção específica de Cryptosporidium canis em amostras fecais de cães. Trezentas amostras fecais de gatos e 367 amostras fecais de cães foram colhidas nas cidades de Araçatuba e Jaboticabal (SP) e submetidas aos processos de purificação e extração de DNA. "Nested" PCR (nPCR) para o gene 18S do rRNA foi realizada para identificação de Cryptosporidium spp., seguido de seqüenciamento dos fragmentos amplificados. Uma reação de PCR em tempo real para um fragmento parcial do gene HSP70 foi padronizada para a detecção de Cryptosporidium canis em amostras fecais de cães. A positividade para Cryptosporidium spp. pela nPCR em amostras de gatos foi de 11,33% (34/300), e em amostras cães foi de 10,4% (38/367). A PCR em tempo real resultou em 15,3% (58/367) de amostras positivas para C. canis. Foi possível a identificação por meio de seqüenciamento de três amostras positivas para C. felis e seis amostras positivas para C. canis. Foi observada uma maior positividade em filhotes quando comparados a cães adultos. A sensibilidade analítica da PCR em tempo real foi de uma cópia de DNA de C. canis por reação e não foi observada amplificação inespecífica de DNA de outras espécies de Cryptosporidium. Conclui-se que cães e gatos das regiões estudad... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract:This study aimed the identification and molecular characterization of Cryptosporidium isolate s in stool samples from dogs and cats, as well as the development of a polymerase chain reaction (PCR) in real time to specific detection of Cryptosporidium canis in fecal samples from dogs. Three hundred fecal samples from cats and 367 fecal samples from dogs were collected in the cities of Araçatuba and Jaboticabal (SP) and subjected to the processes of purification and DNA extraction. Nested PCR (nPCR) for the 18S rRNA gene was performed for identification of Cryptosporidium spp., f ollowed by sequencing of the amplified fragments. A real time PCR to a partial fragment of the HSP70 gene was standardized for detection of Cryptosporidium canis in dogs fecal samples. The positivity for Cryptosporidium spp. by nPCR in cats samples was 11.33% (34/300), and in d og samples was 10.4% (38/367). The real - time PCR resulted in 15.3% (58/367) of samples positive for C. canis . The sequencing of the amplified fragments allowed the identification of three samples positive for Cryptosporidium felis and six samples positive for C. canis . A higher positivity was observed in pup pie s and kittens when compared to adult animals . The analytical sensitivity of real - time PCR was 1 copy of DNA of C. canis and was not observed DNA amplification for other species of Cryptosporidium . We c oncluded that dogs and cats n of the investigated regions can present infected for zoonotic species of Cryptosporidium and the real - time PCR developed in this work is a sensitive and specific method for detection of C. canis in fecal samples from dogs / Doutor

Submitted by (edna.saturno@ufrpe.br) on 2016-08-15T15:26:52Z No. of bitstreams: 1 Debora Rochelly Alves Ferreira.pdf: 381506 bytes, checksum: c2763258d971f97864dfc22e789c9071 (MD5) / Made available in DSpace on 2016-08-15T15:26:52Z (GMT). No. of bitstreams: 1 Debora Rochelly Alves Ferreira.pdf: 381506 bytes, checksum: c2763258d971f97864dfc22e789c9071 (MD5) Previous issue date: 2007-02-15 / The objective of this study was to analyze the clinical-epidemiological situation of the infestation by ectoparasites in domestic felines from João Pessoa city, in the state of Paraiba. Four-hundred and thirty two felines, with no distinction for breed or sex and with different ages, were examined for the presence of external parsites, being 265 domesticated felines from Veterinary Clinics ambulatory routine, Commercial Raising Establishments and domestic collection, in the time period from August 2005 to July 2006; and 167 non-domesticated felines from the Zoonosis Control Center of João Pessoa city (CCZ/JP), that were collected in the time period from October 2005 to July 2006. For the analysis the SAS (StatisticalAnalysis System) software was used, inferential and descriptive statistical techniques were applied, to the significance level of 5% statistics. Considering the total sample, 271 animals were positive for external parasites infestation, being 62.7% the frequency in the studied sample, thus constituting the present study the first contribution to the knowledge of domesticated felines external parasites in the city of João Pessoa – PB. Six species of external parasites were identified on the domesticated and non-domesticated felines, with detected infestation by one or more parasites. The most frequent external parasite species was Ctenocephalides felis (27.3%), followed by Lynxacarus radovskyi (26.2%), Otodectes cynotis (17.4%), Felicola subrostratus (9.7%), Notoedres cati (2.1%) and Rhipicephalussanguineus (1.6%). An association between the infestation by external parasites and domestication, or non-domestication, of the animals was observed, with the existence of a significantly inferior percentage for domesticated animals in comparison to the one obtained for the non-domesticated. The only species of flea found was Ctenocephades felis. No significative difference between the presence offleas and the sex, age, breed, fur color, and fur size parameters was found. The most expressive clinical signs of infestation by fleas were not shiny fur 33.1% (39/118), alopecia 23.7% (28/118), stiff fur 16.9% (20/118) and itching 13.6% (16/118). By evaluating the nutritional condition, greater flea infestation positiveness was observed in animals that were underfed, that condition was statistically significant when the nutritional condition between the studied groups was compared. The obtained results allow us to conclude that the ectoparasitosis constitute important nosological entities in domesticated felines of João Pessoa city, in the state of Paraiba, caused mainly by Ctenocephalides felis and Lynxacarus radovskyi, being the domestication and the nutritional state of the animals the most influent epidemiological factors. / Objetivou-se com este estudo analisar a situação clínico-epidemiológica da infestação por ectoparasitos em felinos domésticos procedentes da cidade de João Pessoa – PB. Foram examinados para presença de ectoparasitos 432 felinos sem distinção de raça, sexo e com idades variadas, sendo 265 felinos domiciliados provenientes da rotina ambulatorial de Clínicas Veterinárias, Criadouros Comerciais e coleta em domicílio, no período de agosto de 2005 a Julho de 2006 e 167 felinos não domiciliados pertencentes ao Centro de Controle de Zoonoses do município de João Pessoa – PB (CCZ/JP), cujas coletas ocorreram a partir de outubro de 2005 a Julho de 2006. Para a análise foi utilizado o “software” SAS (Statistical Analysis System), aplicando-se técnicas de estatística descritiva e inferencial, ao nível de significância de 5% estatística. Considerando-se a amostratotal foram positivos para infestação por ectoparasitos 271 animais, sendo a freqüência de 62,7% na amostra estudada, constituindo-se o presente estudo na primeira contribuição para o conhecimento da ectoparasitofauna de felinos domésticos na cidade de João Pessoa – PB. Foram identificadas seis espécies de ectoparasitos nos felinos domiciliados e não domiciliados, detectando-se infestação por um ou mais parasitos. A espécie de ectoparasito mais freqüente foi Ctenocephalides felis (27,3%), seguido por Lynxacarus radovskyi (26,2%), Otodectes cynotis (17,4%), Felicola subrostratus (9,7%), Notoedres cati (2,1%) e Rhipicephalus sanguineus (1,6%). Observou-se associação entre a infestação por ectoparasitos e a domiciliação ou não dos animais, obtendo-se, para os animaisdomiciliados percentual significativamente inferior ao obtido para os não domiciliados. A única espécie de pulga encontrada foi Ctenocephades felis. Não foi constatada diferença significativa entre presença de pulgas e os parâmetros sexo,idade, raça, cor da pelagem, tamanho da pelagem e cor da pelagem. Os sinais clínicos mais expressivos em infestações por pulgas foram pêlo sem brilho 33,1% (39/118), alopecia 23,7% (28/118), pêlo eriçado 16,9% (20/118) e prurido 13,6% (16/118). Avaliando-se a condição nutricional foi observada maior positividade de infestação por pulgas em animais que apresentavam a condição subalimentado, sendo estatisticamente significativa quando se comparou a condição nutricional entre os grupos estudados. Os resultados obtidos permitem concluir que as ectoparasitoses constituem-se em importantes entidades nosológicas em felinos domésticos da cidade de João Pessoa - PB causadas principalmente porCtenocephalides felis e Lynxacarus radovskyi tendo como fatores epidemiológicos de maior influência a domiciliação e o estado nutricional dos animais.

Submitted by (edna.saturno@ufrpe.br) on 2016-10-10T14:58:02Z No. of bitstreams: 1 Giuliana Viegas Schirato.pdf: 2698527 bytes, checksum: 33a822ba864e7a3747d0b9dd0d006691 (MD5) / Made available in DSpace on 2016-10-10T14:58:02Z (GMT). No. of bitstreams: 1 Giuliana Viegas Schirato.pdf: 2698527 bytes, checksum: 33a822ba864e7a3747d0b9dd0d006691 (MD5) Previous issue date: 2011-02-24 / After skin tumors and lymphohematopoietic tissue, mammary neoplasms are most common in domestic cats. Mammary carcinomas of cats have poor prognosis, since such tumors are very aggressive and with great ability to generate metastases. The aim of this study was to characterize the point of view histopathological, histochemical and immunohistochemical, spontaneously occurring mammary tumors in cats. Thirty-five samples of tumor tissue were collected surgically and histological sections were stained with hematoxylin-eosin (HE) for histopathological analysis. For histochemical analysis, were used the Masson’s Trichrome and Periodic Acid Schiff (PAS) to evaluate the deposition of collagen and glycosaminoglycans associated with tumors, respectively. To perform the immunohistochemistry assays were used anti-Ki-67, anti-PCNA and anti-Bcl-2. The obtained results showed that 14.29% of the lesions were classified as benign (fibroadenoma, adenoma and simple adenosis) and 85.71% as adenocarcinomas. Malignant neoplasms were multifocal, predominantly of invasive growth pattern, presence of necrosis, mitotic figures, and inflammatory infiltrate. The histochemical analysis revealed that the malignant lesions exhibited moderate to strong staining pattern when stained with PAS and weak to moderate with MT. Marking anti-Ki-67 showed cytoplasmic and nuclear pattern in well-differentiated malignant cells in tumor tissue. The protein Bcl-2 showed membrane pattern restricted to the processed tissue and the tissue marked with anti-PCNA showed nuclear and cytoplasmic pattern in glandular cells, occasionally in stromal cells of epithelioid appearance. In summary, the large number of mitotic figures and increased reactivity to staining with anti-PCNA antibody suggests neoplasms with intense proliferative activity indicating a poor prognosis for affected individuals. / Com exceção dos tumores de pele e do tecido linfo hematopoiético, o neoplasmas mamários são os mais comuns em felinos domésticos. Os carcinomas mamários de gatas possuem prognóstico desfavorável, uma vez que tais tumores são bastante agressivos e com grande capacidade de gerar metástases. Objetivou-se com esse estudo, caracterizar sob o ponto de vista histopatológico, histoquímico e imunoistoquímico; neoplasias mamárias de ocorrência espontânea em gatas. Trinta e cinco amostras de tecido neoplásico foram coletadas cirurgicamente e os cortes histológicos foram corados pela Hematoxilina-Eosina (HE), para estudo histopatológico. Para análise histoquímica, utilizou-se o Tricromico de Masson e Ácido Periódico de Schiff (PAS), para avaliar os depósitos de colágeno e glicosaminoglicanos associados aos tumores, respectivamente. Para realização dos ensaios imunoistoquímicos foram utilizados os anticorpos anti-Ki-67, anti-PCNA e anti-Bcl-2. Nos resultados obtidos observou-se que 14,29% das lesões foram classificadas como benignas (fibroadenoma, adenoma simples e adenose) e 85,71% como adenocarcinomas. As neoplasias malignas eram multifocais, de padrão de crescimento predominantemente invasivo, com presença de necrose, figuras de mitose, e infiltrado inflamatório. As análises histoquímicas revelaram que as lesões malignas apresentavam padrão de coloração moderada a forte quando coradas com PAS e fraca a moderada com Tricrômico de Masson. A marcação anti-Ki-67 apresentou padrão citoplasmático e nuclear em células malignas bem diferenciadas no tecido neoplásico. A proteína Bcl-2 apresentou padrão membranar restrito ao tecido transformado e o tecido marcado com o anticorpo anti-PCNA, demonstrou padrão citoplasmático e nuclear em células glandulares, ocasionalmente em células estromais de aspecto epitelióide. Em resumo, a grande quantidade de figuras de mitose e a maior reatividade à marcação com o anticorpo anti-PCNA sugere neoplasmas com intensa atividade proliferativa evidenciando um mau prognóstico para os indivíduos portadores.

Human-wildlife conflict frequently involves carnivores, mainly because of their large home ranges and dietary requirements. As such, carnivores tend to be the first animals to be lost in human-dominated ecosystems. This is significant because the removal of carnivores can alter the functionality of ecosystems. However, the conservation of carnivores depends as much on the socio-political and socio-economic landscapes as it does on the ecological one. The consolidation of vast, un-fragmented conservation areas in Africa and the world is unlikely. Thus, unravelling the factors (both biological and sociological) responsible for and influencing human-predator conflict is critical for carnivore conservation. The Winterberg district in the Eastern Cape, South Africa has been a sheep (Ovis aries) farming stronghold for nearly 200 years. Consequently, conflict between farmers and predators is commonplace and depredation of livestock by caracals (Caracai caracal) remains a perennial problem. However, the extent of this human-predator conflict (including a reliable assessment of caracal density) has not been quantified. This study used camera trapping to estimate the density of caracals (a nonindividually recognisable species) in the Winterberg and a structured questionnaire to gauge the general attitudes of the farmers of the region. Caracal density was estimated to be 0.20 caracals/km². This estimate equates to a population of approximately 54 caracals across the entire district. Therefore, caracals do not appear to occur at high densities in the Winterberg. However, the survey of the residents of the Winterberg Conservancy revealed that predators were rated as the greatest problem faced by farmers in the area. Although not significant, it also showed that the size of a respondent's property and sheep mortality due to caracals had the greatest probability of affecting attitudes towards predators. The farmers lost less than 10% of their stock to caracal depredation on an annual basis. Therefore, the general perception that predators were the most important factor in stock loss in the area does not appear to be fully supported by my data. The Winterberg is a male-dominated, multigenerational society and it is likely this propagates certain perceptions towards predators which are a reflection of long-held family traditions and beliefs. Such situations can result in reactions which are disproportionate to the actual scale of the problem. However, my study was limited to a sub-set of the landowners in the Winterberg. Thus, more intensive assessments of caracal space use and community attitudes towards predators are recommended.

Feline babesiosis, first described in domestic cats in South Africa in 1937, is regarded to be of great importance in the coastal regions although isolated cases also occur on the eastern highlands of Mpumalanga Province. Babesia felis (described from domestic cats) and B. leo (described from lions) are the two best characterised Babesia species in felids. These two parasites are morphologically similar when examined under a light microscope, but are serologically and genetically distinct. In this study the prevalence of these two Babesia species in various wild and domestic felid species was determined. A total of 358 samples were tested using the reverse line blot hybridization (RLB) assay. This assay makes it possible to simultaneously detect and differentiate between blood parasites using DNA probes. The RLB consists of three basic steps, the first being amplification of the variable region (V4) in the 18S rRNA gene using genus-specific primers where one is labelled with biotin. This is followed by a blotting step, where the amplicons are hybridized to oligonucleotides bound to a nitrocellulose membrane. The third and last step is the detection of the hybridized amplicons by using chemiluminescence reagents. This assay is a screening tool utilizing the variable (V4) region in the 18S rRNA gene to detect and differentiate between blood parasites. A new B. felis-specific DNA probe was developed to use in the RLB assay. Results demonstrated that these two parasites not only occur in the felid species from which they have been described, but also in other felid species. Babesia microti was also detected in various felid species, while B. rossi was detected in 1 of the lion samples. Two hundred and twelve samples tested positive for Babesia spp., of which only 54.24% of the samples reacted with the genus-specific probe. This indicates the presence of a novel Babesia or Theileria species or variant of a species. / Dissertation (MSc)--University of Pretoria, 2010. / Veterinary Tropical Diseases / unrestricted

No description available.

De nombreux parasites circulent dans les populations naturelles. Au sein d’un hôte, souvent pluri-infecté, ils peuvent interagir, augmentant ou réduisant le risque d’infection et les symptômes d’autres pathogènes. L’étude de ces interactions commence seulement dans les populations naturelles. Les enjeux sont cruciaux : détecter les interactions d’intérêt, estimer la probabilité de coinfection, comprendre la cocirculation des parasites. La détection des interactions sur le terrain est compliquée par la nature des données (e.g., présence-absence) et les facteurs confondants créant des associations statistiques (fausses interactions). Ce travail visait à mener une réflexion transversale sur ces interactions et le multiparasitisme, avec des applications à des données sérologiques pour 4 virus félins suivis dans des populations rurales de chats domestiques. De nouvelles méthodes de modélisation dynamique et statistique ont été développées pour prendre en compte les facteurs générant de fausses interactions (effet cumulatif de l’âge, facteurs de risque communs) et évaluer le biais des méthodes classiques. Des synergies entre 3 couples de virus félins ont été révélées. On a aussi identifié des caractéristiques comportementales et physiologiques (modes de vie, niveau de testostérone), qui, en modulant l’exposition et la sensibilité aux pathogènes, génèrent une forte hétérogénéité entre les hôtes. Enfin, une vision intégrative des systèmes hôte-parasites est indispensable pour appréhender la complexité des communautés et évaluer l’impact de la multitude d’hôtes, de parasites et d’interactions sur la coévolution, la conservation des espèces et la gestion des maladies infectieuses / Numerous parasites circulate within natural host populations. Within a host, often pluri-infected, parasites can interact, increasing or decreasing the infection risk and/or symptoms’ severity of other pathogens. Studies of such interactions only start in natural populations. Their stakes are high: detecting interactions of interest, estimating coinfection probabilities and understanding the cocirculation of parasites. The detection of interactions in the field is however complicated by the nature of data (often presence-absence) and the existence of confounding factors that can create statistical associations (false interactions). This work aimed at having a cross-cutting reflection on those interactions and on multiparasitism, with applications on a rich dataset of four feline viruses followed in rural populations of domestic cats. New dynamical and statistical modeling methods were developed to take into account factors generating false interactions (cumulative effect of age, shared risk factors) and evaluate the biases of classical methods. Synergies between three pairs of feline viruses were revealed. In addition, we identified behavioral and physiological factors (e.g., way of life, testosterone levels) that, by modulating exposition and/or susceptibility to pathogens, generate strong heterogeneity between hosts. Finally, a more integrative approach to host-parasites systems is proposed. It now appears necessary if one wants to deal with communities’ complexity and further evaluate the impact of multiple hosts, multiple parasites and their interactions on their coevolution, species conservation and infectious diseases management

Le chat haret est l’un des prédateurs invasifs les plus dommageables pour la biodiversité insulaire. Sa présence est associée à une perte de biodiversité sur l’ensemble des îles sur lesquelles il est établi, et où il constitue une menace pour de nombreuses espèces de vertébrés souvent endémiques et menacés. En Nouvelle-Calédonie des populations de chats harets sont présentes dans tous les milieux et habitats et l’étude de son écologie et de ses impacts sur la faune ont fait l’objet de ce travail de thèse. L’analyse du régime alimentaire sur 14 sites d’études représentatifs des 4 habitats majeurs a révélé un régime très diversifié et une forte prédation sur les vertébrés natifs et notamment sur le groupe des scinques, des roussettes et des pétrels. Parmi les 44 espèces de vertébrés retrouvées dans le régime alimentaire de ce prédateur invasif, la plupart sont endémiques et 20 listées comme menacées sur la liste rouge mondiale de l’UICN. Le suivi des déplacements d’individus équipés de colliers GPS au niveau d’une presqu’île abritant une importante colonie d’oiseaux marins, a permis de mettre en évidence de grands domaines vitaux pour les mâles, des domaines vitaux petits pour les femelles et des patrons de déplacements liés aux différentes étapes du cycle reproducteur des oiseaux marins. Ces éléments, couplés aux analyses de régime alimentaire suggèrent une prédation à une large échelle géographique, à la fois sur les adultes reproducteurs mais également sur les jeunes oiseaux proches de l’envol. Une opération expérimentale de contrôle d’une population de chats harets a été conduite sur cette presqu’île et a montré une faible durabilité des effets de la suppression des individus sur les densités observées et une rapide recolonisation du site. Les résultats de ce travail plaident pour la mise en place de mesures de limitation des impacts occasionnés et permettent en particulier de cibler les habitats de maquis et forêt humide comme prioritaires en matière de limitation des abondances. / Feral cat (Felis catus) is one of the most successful and harmful invasive predator species for island biodiversity. The presence of this alien predator species generally lead to dramatic loss of native island biodiversity and represents a serious threat for numerous endemic and threatened species. Feral cats have invaded the whole New-Caledonian archipelago and all its habitats. In this study, we focused on the ecology and impacts of this invasive predator on the outstanding endemic fauna found in the different habitats of the exceptional New-Caledonia biodiversity hotspot. Feral cat diet analyses on 14 selected sites representing the 4 main natural habitats revealed a high diversified diet and high predation rates on native species particularly on squamates, flying foxes and petrels. Among the 44 vertebrates species found into the feral cat diet, 20 are IUCN red-listed threatened species. Cat movements of eleven feral cats fitted with GPS collars have been studied in a western coast Peninsula hosting an important seabird colony. Male cats showed large home ranges while female showed small home ranges. Feral cats exhibited important movements within the studied peninsula linked with the breeding cycles of seabirds. GPS data coupled with dietary informations suggested a predation that concerned both breeding adults and fledgings bird, and at a large geographic scale as some feral cats have their core home range distant to the colony (>3km). We evaluated the effects of a high level but intense cat control on this site that showed a low sustainability of feral cat culling and a rapid recolonization process. Our results are pleading for the future limitation of feral cat impacts and call to focus first abundance limitation measures on maquis mosaic and humid forest habitats This study also provided information on the spatial extent and intensity of future control measures in the special context of a large and highly invaded island.

Made available in DSpace on 2016-04-28T20:15:32Z (GMT). No. of bitstreams: 1 2009 - Gisele Santos de Meireles.pdf: 1867896 bytes, checksum: 50898c1a65b34d41d9d4352322ce8d1e (MD5) Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This work aimed, to determine the phenotypic analysis from sporulated oocysts of Cystoisospora felis by using a sequential method of cleaning techniques. By using SDS-PAGE at 10 and 12 % were separated 22 protein groups as: 26.65; 29.71; 31.79; 36.41; 44.03; 50.09; 56.08; 62.64; 73.65; 77.55; 85.34; 97.62; 98.66; 101.24; 104.21; 109.23; 110.56; 113.21; 138.48; 180.50; 206.81 and 244.51KDa belonged to estrutucture from C. felis sporulated oocysts. Western Blotting technique was performed after SDS-PAGE and were identified these antigenic proteins: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 and p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa from hyperimmune and natural infected rabbits by C. Felis respectively. / Este trabalho teve por objetivo, tra?ar um perfil prot?ico de oocistos esporulados de Cystoisospora felis, recuperados a partir do uso seq?encial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 10 e 12 % resultou na identifica??o de 22 grupos prot?icos de 26; 29; 31; 36; 44; 50; 56; 62; 73; 77,55; 85,34; 97,62; 98,66; 101,24; 104,21; 109,23; 110,56; 113,21; 138,48; 180,50; 206,81 e 244,51 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de coelho heter?logo anti-C. felis foi poss?vel desenvolver uma t?cnica de diagn?stico imunoenzimatico com western Blotting para identifica??o de animais infectados de maneira natural ou experimental com C. felis, identificando os seguintes prote?nas antig?nicas: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 e p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa, respectivamente.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-10-24T18:46:15Z No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) / Made available in DSpace on 2018-10-24T18:46:15Z (GMT). No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) Previous issue date: 2013-02-27 / CAPES / FAPERJ / This study aimed to determine from the protein profile of oocysts of Cystoisospora felis recovered from the sequential use of various purification techniques adapted for specific use in sporulated oocysts of C. felis. With the aid of SDS-PAGE 12% resulted in identification of 25 groups of protein: 266, 240, 186, 165, 140, 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 kDa belonging to the structure of sporulated oocysts and sporozoites of C. felis. Based on this results and heterologous bovine serum anti-C. felis was possible to determine polypeptides dominant relevant to diagnostic immunoassay technique with "Western Blotting", these being immunodominant bands: P208, P138, P113, p106, p62, p56, p51, p48, p44, p38, p36, and p33 p27. In order to avoid misdiagnosis from cross-reactivity a positive control serum anti-C. felis was compared to with positive serum anti-Toxoplasma and Neospora in order to exclude the common protein bands, probably markers of gender and group to identify cattle infected naturally or experimentally with C. felis. As showed, the following specific antigenic protein units: p 206-208, P137-139, p112- 113, p104-107, p27-28 are responsible for determining the animal tested positive or not for Cystoisospora felis. Of the analysis of the variables could be observed that the presence of felines related to the handling, size and type of milking properties facilitates dispersion C. felis. / Este trabalho teve por objetivo, diagnosticar a infec??o por Cystoisospora felis em bovinos atrav?s do Western Blotting, apartir de oocistos obtidos com o uso sequencial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 12 % resultou na identifica??o de 25 grupos proteicos de: 266; 240; 186; 165; 140; 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de bovino heter?logo anti-C. felis foi poss?vel determinar os polipept?deos dominantes relevantes ? t?cnica de diagn?stico imunoenzimatico com ?Western Blotting?, sendo estas, as bandas imunodominantes: p208, p138, p113, p106, p62, p56, p51, p48, p44, p38, p36, p33 e p27. A fim de evitar o diagn?stico equivocado a partir de rea??es cruzadas foi feita a compara??o do soro controle positivo anti-C. felis com o soro positivo anti-Toxoplasma e Neospora com o intuito de excluir as bandas proteicas comuns, prov?veis marcadoras de g?nero e grupo para identifica??o de bovinos infectados de maneira natural ou experimental com C. felis. Sendo evidenciadas, as seguintes unidades proteicas antig?nicas espec?ficas: p 206-208, p137-139, p112-113, p104-107, p27-28 respons?veis por determinar a positividade dos animais testados para C. felis. A partir das an?lises das vari?veis foi poss?vel observar que a presen?a de felinos associados, ao manejo, tipo de ordenha e tamanho das propriedades facilita a dispers?o de C. felis.