FundaÃÃo Cearense de Apoio ao Desenvolvimento Cientifico e TecnolÃgico / CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Estudo farmacolÃgico do Ãleo essencial de Croton nepetaefolius Baill. sobre o mÃsculo liso traqueal e vascular e sobre as propriedades eletrofisiolÃgicas de neurÃnios fÃsicos de gÃnglio celÃaco. Pedro Jorge Caldas MagalhÃes, Tese de Doutorado em Farmacologia, UFC, 2002. Croton nepetaefolius Baill à um arbusto aromÃtico do Nordeste brasileiro, conhecido como âmarmeleiro sabiÃâ, utilizado na medicina popular como antiespasmÃdico e carminativo. Os principais constituintes do seu Ãleo essencial sÃo 1,8-cineol, metil-eugenol, xantoxilina e terpineol. Recentemente, demonstraram-se propriedades antiespasmÃdica intestinal, hipotensiva, antiinflamatÃria e analgÃsica para o Ãleo essencial do Croton nepetaefolius (OECN). Neste trabalho, caracterizamos os efeitos farmacolÃgicos do OECN sobre o mÃsculo liso respiratÃrio e vascular de rato e cobaio e sobre o funcionamento elÃtrico de neurÃnios de gÃnglio celÃaco de cobaio. Usamos preparaÃÃes in vitro de vasos sanguÃneos de ratos e cobaios machos (aorta e vasos mesentÃricos) e anÃis de traquÃia, mantidos em soluÃÃo nutridora, aerada, pH 7,4, a 37 oC, para registro isomÃtrico das contraÃÃes musculares. Usamos tambÃm gÃnglios celÃacos intactos de cobaios, mantidos em temperatura ambiente in vitro, para os registros eletrofisiolÃgicos pela tÃcnica do microeletrodo intracelular. In vivo, avaliamos as aÃÃes do OECN sobre a pressÃo arterial mÃdia e alguns parÃmetros cardÃacos, respiratÃrios e hematolÃgicos em ratos. O OECN (0,1 - 1000 microgrma/ml) relaxou o tÃnus basal (5 mM [K+]) e o tÃnus aumentado por K+ (60 mM) em traquÃia de cobaio, de maneira dependente de concentraÃÃo (CE50 de 4 e 63 micrograma/ml, respectivamente). Em tecidos de cobaios previamente sensibilizados, o OECN (300 e 600 micrograma/ml), reduziu a contraÃÃo induzida pela apresentaÃÃo do antÃgeno sensibilizante (ovalbumina). Na faixa de concentraÃÃo de 100 a 400 micrograma/ml, bloqueou as contraÃÃes induzidas por histamina e PGF2 alfa. O OECN inibiu as contraÃÃes induzidas por histamina, carbacol e KCl com CI50 na faixa de 100 - 130 micrograma/ml. Em aorta de rato e cobaio, relaxou a contraÃÃo induzida por 60 mM de K+ (CI50 de 32 e 200 micrograma/ml, respectivamente). Em rato, mas nÃo em cobaio, este relaxamento foi parcialmente inibido pela retirada do endotÃlio vascular ou pela adiÃÃo de 100 micrograma M de L-NAME. Em aorta de cobaio, o OECN inibiu as contraÃÃes independentes de Ca2+, induzidas por dibutirato de forbol e por K+ hiperosmolar na soluÃÃo nutridora. O OECN diminuiu preferencialmente a pressÃo arterial em ratos DOCA-sal do que em ratos nefrectomizados e, bloqueou mais potentemente as contraÃÃes induzidas pela fenilefrina em aorta de rato DOCA do que dos animais nefrectomizados. O OECN, metil-eugenol e terpineol, aumentam o fluxo de lÃquido pela circulaÃÃo mesentÃrica de rato, sendo este efeito parcialmente inibido pela presenÃa de L-NAME. Em nenhuma dessas preparaÃÃes o OECN produziu hiperpolarizaÃÃo do potencial transmembrana. Em neurÃnios fÃsicos de gÃnglio celÃaco de cobaio, o OECN diminuiu significativamente o aumento da excitabilidade produzido pela histamina sem alterar as propriedades passivas e ativas dos neurÃnios. Em conclusÃo, o OECN relaxa o mÃsculo liso das vias aÃreas, à um agente hipotensor e vasorelaxante e diminui a excitabilidade induzida por histamina em neurÃnios autonÃmicos. Seus efeitos sÃo, provavelmente, intracelulares ou mediados por proteÃna quinase C. / Croton nepetaefolius is an aromatic bush found in brazilian Northeast region, called âmarmeleiro sabiÃâ, and it is used in folk medicine as an antispasmodic and carminative agent. Its essential oil is comprised of 1,8-cineole, methyl-eugenol, xanthoxylin, terpineol and others constituents. Recent studies showed some pharmacological activities of the essential oil of Croton nepetaefolius (EOCN) as an intestinal antispasmodic, hypotensive, anti-inflammatory and analgesic agent. Our aim in this work was to evaluate the effects of EOCN on airway and vascular smooth muscle and also on autonomic neurons. We used in vitro models of rat and guinea-pig isolated vessels and guinea-pig tracheal rings for isometric recording of the smooth muscle contractions. Guinea-pig celiac ganglion, was used for intracellular microelectrode recording of electricophysiological signals. Moreover, mean arterial pressure, cardiovascular, respiratory and hematologic parameters were measured in vivo in rats. EOCN (0,1 â 1000 microgram/ml) relaxed basal and K+-increased guinea-pig tracheal tonus (EC50 = 4 and 63 microgram/ml, respectively), in a concentratation-dependent manner. In ovalbumin-sensitized guinea-pig tissues, EOCN inhibited the antigen-induced contraction. EOCN (100 - 400 microgram/ml) blocked the histamine- and PGF2alpha -induced contractions. The contractions induced by histamine, carbacol and KCl were inhibited by EOCN with IC50s between 100-130 microgram/ml. In rat and guinea-pig aortic rings, EOCN relaxed the 60 mM K+-induced contractions (IC50 = 32 and 200 microgram/ml, respectively). Only in rat tissues, this EOCN-induced relaxation was partially inhibited by both L-NAME (100 microgram M) or endothelium lack. In guinea-pig aortic rings, EOCN inhibited the Ca2+-independent phorbol esther- and hyperosmotic K+- induced contractions. EOCN, preferably, diminished the mean arterial pressure and inhibited the aortic rings phenylephrine-induced contractions in DOCA-salt treated rats rather than uninephrectomized rats. Both EOCN, methyl-eugenol and terpineol increased the flow through rat mesenteric bed. This effect was partially blocked by L-NAME (50 microgram M). EOCN did not produce hyperpolarization of the transmembrane potential. In celiac ganglion phasic neurons, EOCN signicantly inhibited the histamine-induced increase of the neuronal excitability. In conclusion, EOCN is an airway smooth muscle relaxant, hypotensor and vasorelaxant agent, and it is a blocker of the stimulant histamine activity on autonomic neurons. Its effects are, probably, mediated by an intracellular action or protein C kinase modulation.

INTRODUÇÃO: A importância do parênquima pulmonar na piora funcional da asma tem sido recentemente investigada. Embora a ativação da enzima óxido nítrico sintase induzida (iNOS) amplifique a responsividade e o remodelamento das vias aéreas induzidos pela inflamação crônica, seu efeito no parênquima pulmonar não foi previamente estudado. OBJETIVO: Avaliar a influência do óxido nítrico derivado da iNOS na mecânica pulmonar, na inflamação e no processo de remodelamento no tecido pulmonar periférico de cobaias com inflamação pulmonar alérgica. MÉTODOS: Os animais foram submetidos a sete inalações com doses crescentes de ovalbumina (1~5 mg/mL) ou soro fisiológico por 4 semanas. As cobaias receberam 1400-W (inibidor específico de iNOS, intraperitoneal) ou veículo por 4 dias, iniciando 30 minutos antes da sétima inalação. Após 72h da sétima inalação, os animais foram anestesiados, exsanguinados e fatias de tecido pulmonar periférico foram retiradas e suspensas em banho orgânico de Krebs, e a resistência e elastância tecidual foram avaliadas em condição basal e após desafio com ovalbumina. Após, as fatias de tecido pulmonar periférico foram submetidas à avaliação histopatológica. RESULTADOS: Os animais expostos às inalações com ovalbumina apresentaram valores maiores de porcentagem de aumento da resistência e da elastância tecidual em relação ao basal após desafio com ovoalbumina no banho (p<0.05). Houve aumento no número de eosinófilos (p<0.001), nas células iNOS positivas (p<0.001), na deposição de fibras elásticas e colágenas (p<0.05), na densidade de actina (p<0.05) e na expressão de 8-epi-PGF2a (p<0.001) no septo alveolar. A administração de 1400-W reduziu todos estes parâmetros funcionais e morfológicos (p<0.05). CONCLUSÕES: Neste modelo experimental, o bloqueio específico da iNOS atenuou a constrição, a inflamação e o remodelamento no parênquima pulmonar. Estas alterações podem estar relacionadas aos efeitos do óxido nítrico na modulação da via do estresse oxidativo. O presente estudo sugere que a inibição específica da iNOS pode amplificar as estratégias terapêuticas utilizadas na abordagem de doenças inflamatórias crônicas pulmonares. / INTRODUCTION: The importance of lung parenchyma in functional asthma impairment has been recently addressed. Although the inducible nitric oxide synthase (iNOS) activation amplifies chronic inflammation-induced airway responsiveness and remodeling, its effect on lung parenchyma has not been previously investigated. OBJECTIVE: To evaluate the influence of iNOSderived NO in the pulmonary mechanics, inflammation, and remodeling processes in peripheral lung tissue of guinea pigs with pulmonary allergic inflammation. METHODS: Animals were submitted to seven ovalbumin exposures with increasing doses (1~5 mg/mL) or saline for 4 weeks. The guinea pigs received 1400-W (iNOS-specific inhibitor, intraperitoneal) or vehicle for 4 days, beginning 30 minutes before the 7th inhalation. At 72h after the 7th inhalation, animals were anesthetized, exsanguinated and peripheral lung tissue strips were retreat and suspended in a Krebs organ bath, and the tissue resistance and elastance were evaluated at baseline condition and after ovalbumin challenge. After that, strips were submitted to histopathological evaluation. RESULTS: The ovalbumin-exposed animals presented greater values of percentage of increase of tissue resistance and elastance related to baseline after ovalbumin challenge in the bath (p<0.05). There were increase in the number of eosinophils (p<0.001) and iNOSpositive cells (p<0.001), in collagen and elastic fiber deposition (p<0.05), in actin density (p<0.05) and in 8-epi-PGF2a expression (p<0.001) in the alveolar septa. The 1400-W administration reduced all these functional and morphological parameters (p<0.05). CONCLUSIONS: In this experimental model, the iNOS-specific blockage attenuated constriction, inflammation, and remodeling in the lung parenchyma. These alterations may be related to NO effects in the modulation of the oxidative stress pathway. The present study suggests that specific iNOS inhibition can amplify the therapeutics strategies used in the management in chronic inflammatory lung diseases.

O nervo vestíbulo-coclear da cobaia possui peculiaridades não encontradas em outros nervos periféricos. Não foram encontradas informações detalhadas sobre os aspectos morfométricos do VIII nervo craniano em cobaias adultas na literatura. A avaliação descritiva e quantitativa no presente estudo, evidencia informações que precedem o estudo das alterações que ocorrem em modelos experimentais de neuropatias do VIII nervo craniano e as doenças ou lesões que possam afetar o homem. Foram utilizadas 8 cobaias adultas, perfundidas com glutaraldeído a 2,5% em tampão cacodilato de sódio (0,025 M). Seus nervos direito e esquerdo (n=6, 4 nervos direito e 2 esquerdos) foram dissecados na região mediana do nervo e pós fixado com tetróxido de ósmio (OsO4) a 1% e incluídos em resina epóxi Poly/Bed 812®. Os fragmentos foram cortados em secções transversais semifinas seriadas (6 nervos) com uma espessura de 0,5 &#956;m e corados com azul de toluidina para a microscopia de luz. Foram analisados os aspectos histológicos descritivos e topográficos do nervo vestíbulo-coclear em cobaias adultas, bem como os aspectos histométricos na parte mediana do nervo, no referente a densidade de fibras mielínicas, a distribuição dos diâmetros de tal tipo de fibras mielínicas, ao cálculo da razão G e o número de vasos sangüíneos encontrados dentro da área de cada nervo. O nervo coclear é envolvido pelo vestibular na porção inicial da junção de ambos. O conjunto, nervos coclear e vestibular e nervo vestíbulo-coclear têm a aparência bifurcada da letra Y, na horizontal com os ramos lateralizados, possuindo em média 5 mm de comprimento. É ricamente vascularizado e documenta uma completa interposição das fibras dos nervos coclear e vestibular, quando o fascículo do VIII nervo está completamente constituído. As suas fibras mielínicas dispõem-se paralelamente em seu sentido longitudinal e são circundadas por escasso tecido endoneural. Não foram evidencias fibras amielínicas no VIII nervo. A distribuição das fibras mielínicas no segmento mediano do VIII nervo craniano foi unimodal, havendo predomínio de fibras com 3,5 &#61549;m de diâmetro. O número de fibras foi de 5.390 ± 1.504 fibras. O diâmetro das fibras variou de 1,5 a 6 &#61549;m de diâmetro. Os axônios com 2,5 &#61549;m de diâmetro foram predominantes quantitativamente: 6.757 ± 1.922 axônios. O diâmetro axonal variou entre 1 a 4,5 &#61549;m. A densidade média das fibras do VIII nervo em seu segmento mediano foi de 41.474 ± 4384 fibras/mm2. O diâmetro fascicular foi de 0,77 ± 0,04 mm. A razão G varia de 0,2 a 0,9. O maior número de fibras tem razão G de 0,7 e 0,6, respectivamente, predominando as fibras com 0,7. Os resultados descritos neste trabalho científico são originais, contendo alguns resultados inéditos e pouco estudados na literatura científica sobre a anatomia e morfometria do VIII nervo craniano em cobaia. O nervo vestíbulo-coclear da cobaia é um nervo sensitivo aferente especial, e bem diferente em sua anátomo-fisiologia de todos os outros nervos sensitivos e motores encontrados nos mamíferos. A cobaia adulta é um excelente modelo experimental para o estudo do nervo vestíbulo-coclear, semelhantes aos de humanos. / The vestibulocochlear (VIIIth cranial nerve) nerve of guinea pigs have special features not common for peripheral nerves in general. There are no detailed reports on the morphometric characteristics of the VIII nerve in adult guinea pigs. This is a descriptive and qualitative study that shows normal parameters that are useful in experimental models of the VIIIth nerve neuropathy. Eight adult guinea pigs were perfused with 2.5% glutaraldehyde in isotonic cacodilate buffer (0.025M). The right (N=4) and left (N=2) nerves were dissected in the median region, post-fixed in 1% osmuin tetroxide and embedded in epoxy resin. Semithin (0.5 µm) serial transverse sections were stained with 1% toluidine blue for light microscopy study. The descriptive histology and the topographical and histometric aspects of the median region of the nerve were analyzed, including myelinated fiber density, myelinated fiber diameter distribution, g ratio and number of endoneural capillary vessels. The cochlear nerve is enveloped by the vestibular nerve when they join together. Both, the cochlear and the vestibular nerves join to form the vestibulocochlear nerve with a Y shape, with approximately 5 mm in length. The nerve is widely vascularized and presents a complete mixture of the vestibular and cochlear myelinated fibers when the VIII nerve fascicle is constituted. The myelinated fibers are longitudinally oriented and present few endoneural connective tissue in between. No unmyelinated fibers were evidenced in the VIII nerve. The myelinated fiber diameter distribution was unimodal, with a peak at 3.5, and intervals between 1,5 and 6 &#61549;m. The average number of fibers was 5.390 ± 1.504. The myelinated axon diameter distribution was also unimodal, with a peak at 2.5 µm of diameter were predominant quantitatively: 6.757 ± 1.922, and intervals between 1 and 4.5 µm. The average myelinated fiber density was 41.474 ± 4384 mm2, spread in a fascicular diameter of 0.77 ± 0.04 mm. The g ratio values varied from 0.2 to 0.9 and most of the myelinated fibers showed g ratio values of 0.7. Our results are original and only few parameters studied here were described in the literature. The vestibulocochlear nerve of the guinea pigs is a special afferent nerve, differing from other peripheral nerves (sensitive and motor) in its main anatomic and physiological characteristics, compared to other nerves from mammals. The adult guinea pig is an excellent model for the vestibulocochlear nerve neuropathy studies once it is similar to the human nerve.

Há evidências de que os endocanabinoides e os receptores CB1 estejam envolvidos em diversos transtornos emocionais, dentre eles a ansiedade e a depressão. A interação deste sistema endocanabinoide com outros neurotransmissores, como a serotonina (5-HT), tem sido alvo de diversos estudos, uma vez que o aumento na atividade deste sistema promove respostas ansiolíticas e antidepressivas. Alguns estudos mostraram que a porção ventromedial do hipotálamo modula algumas respostas defensivas, como fuga e a imobilidade tônica, respostas estas eliciadas pelos animais frente a situações de medo intenso, como ocorre em um confronto com um dado predador. Dentro desta perspectiva, o presente estudo teve por objetivos avaliar o efeito do tratamento crônico durante 21 dias com canabidiol (CBD) sobre as respostas defensivas em cobaias (Cavia porcellus), evocadas diante de um predador natural. Avaliamos, ainda, o efeito da microinjeção intradiencefálica de AM251 (antagonista de receptores CB1; 100pmol/0,2µl), de 6-I-CPS (antagonista de canais iônicos TRPV1; 9nmol/0,2µl), de WAY-100635 (antagonista 5-HT1A; 0,37nmol/0,2µl) e seus respectivos veículos em diferentes grupos de cobaias, após o tratamento crônico com canabidiol sobre as respostas defensivas evocadas diante do predador. Os resultados mostraram que, muito embora o tratamento crônico com CBD não tenha atenuado a resposta de imobilidade tônica (IT), devido ao fenômeno de habituação da resposta, diminuiu a expressão de outras respostas comportamentais, como a atenção defensiva e afuga orientada para a toca, promovendo, pois, um efeito anxiolítico e panicolítico. Ademais, a microinjeção de AM251 tendeu a abolir o efeito ansiolítico causado pelo tratamento crônico com CBD, potencializando as respostas defensivas diante de um predador natural. Tais resultados indicam que o CBD promove seu efeito farmacológico também mediante tratamento crônico, e que os receptores canabinoides do tipo CB1 do hipotálamo ventromedial parecem desmpenhar algum um papel nesse mecanismo de ação. / Evidence has shown that endocannabinoids and CB1 receptors are involved in several emotional disorders, including anxiety and depression. The endocannabinoid system and its interaction with other neurotransmitters such as serotonin (5-HT) has been the subject of several studies, since the increase in the activity of this system promotes anxiolytic and antidepressant responses. Some studies have shown that the ventromedial division of the hypothalamus modulates some defensive responses such as flight and tonic immobility, elicited by preys experiencing intense fear-like reactions when facing dangerous situations, as confrontation with a given predator. From this perspective, this study aimed to evaluate the effect of chronic treatment for 21 days with cannabidiol (CBD) on the defensive responses displayed by guinea pigs (Cavia porcellus), in the presence of a natural predator. We also studied the effect of intradiencephalic microinjection of AM251 (a CB1 receptor antagonist; 100pmol / 0.2?L), 6-PSC-I (TRPV1 ion channel antagonist; 9nmol / 0.2?L), or WAY- 100635 (antagonist 5- HT1A; 0.37nmol / 0.2?L) and their respective controls in different groups of mice after chronic treatment with cannabidiol on the defensive responses evoked in the presence of the predator. The results showed that although the chronic treatment with CBD attenuated tonic immobility response (IT) and other behavioural responses, such as defensive attention and oriented escape behaviour, promoting a significant anxiolytic and panicolytic effect. In addition, intra-hypothalamic microinjection of AM251 exert a potential impairment of the antipanic effect caused by chronic treatment with CBD, increasing the defensive responses displayed in the presence of the predator. These results indicate that the CBD also promotes its pharmacological effect upon chronic treatment, and that medial hypothalamus CB1 receptors seem to play a role in its mechanism of action.

A União Econômica e Monetária do Oeste Africano (UEMOA) é uma organização de integração regional constituída por oito países da África Ocidental: Benim, Burkina Faso, Costa do Marfim, Guiné Bissau, Mali, Níger, Senegal e Togo. O presente trabalho tem por objetivos realizar: (a) a classificação climática segundo Köppen (1900) e Thornthwaite (1948), e (b) o zoneamento agrícola das culturas de milho e soja na região da UEMOA com base nos valores de (i) produtividade potencial (dióxido de carbono, temperatura, radiação fotossinteticamente ativa, fotoperíodo e genótipo), (ii) produtividade atingível, com elevado uso de insumos e tecnologia e (iii) produtividade real. A classificação climática de acordo com ambos os autores caracterizou a maior parte do território formado pela UEMOA como árido e em relação ao zoneamento agrícola, tem-se que a região da UEMOA é altamente indicada para o cultivo de milho e de soja, contudo, os valores médios de produtividade real referentes a esses cultivos são inferiores aos valores da produtividade atingível, indicando que o principal problema na região é o baixo nível de adoção tecnológica. / The West African Economic and Monetary Union (also known as UEMOA from its name in french: Union Économique et Monétaire Ouest-Africaine) is an organization of eight West African countries: Benin, Burkina Faso, Ivory Coast, Guinea-Bissau, Mali, Niger, Senegal and Togo. The following study has as main objectives: (a) climate classification by Köppen e Thornthwaite; and (b) agricultural zoning for maize and soybean in the UEMOA region based on (i) potencial yield (carbon dioxide, temperature, photosyntheticaly active radiation, photoperiod and genotype), (ii) attainable yield, under high inputs and technological adoption and (iii) actual yield. Climate classification according to both authors characterized most of the territory formed by UEMOA as arid and regarding to agricultural zoning, UEMOA region is highly suitable for growing maize and soybeans, however, average values of actual yield in relation to these crops are lower than those of attainable yield, indicating that the main problem in this region is the low level of technological adoption.

Submitted by Maria Dulce (mdulce@ndc.uff.br) on 2014-02-06T17:54:03Z No. of bitstreams: 1 Santos, Beatriz-Dissert-2013.pdf: 979494 bytes, checksum: ce044e3590d5d82f2249b94be9745a9d (MD5) / Made available in DSpace on 2014-02-06T17:54:03Z (GMT). No. of bitstreams: 1 Santos, Beatriz-Dissert-2013.pdf: 979494 bytes, checksum: ce044e3590d5d82f2249b94be9745a9d (MD5) Previous issue date: 2013 / nserida no contexto das discussões sobre o Ultramar e do chamado Mundo Atlântico, a região denominada pelos portugueses de “Guiné do Cabo Verde” reconhecidamente apresentou desde os primeiros contatos, durante o período expansionista português, características singulares. No entanto, o foco sobre o estudo da cultura da Guiné parece ter sido sempre ofuscado pelas curiosidades e potencialidades que as interações, de diversas naturezas, entre europeus, africanos e árabes geravam. Dessa forma, apresenta-se nesta dissertação um estudo que visa dar um passo em direção ao preenchimento dessa lacuna deixada em aberto. Para este fim, o objetivo aqui é o de contextualizar a região no período dos séculos XVI e XVII e seu lugar dentro da lógica do mundo Ultramarino. Tendo como proposta principal promover uma análise sobre um dos grupos étnicos mais conhecidos do período, os mandingas. Esta proposta utiliza como fontes os relatos de três viajantes cabo-verdianos que comerciaram na região durante décadas. Assim apresenta-se aqui uma análise reflexiva a respeito de vários assuntos pertinentes a temática de estudos da cultura, história da África e das imagens deixadas aos historiadores, por meio das fontes, do período da expansão marítima / Inserted in the context of discussions on the so-called Ultramarine and the Atlantic World, region called by the Portuguese “Guinea of the Cape Verde” admittedly showed unique characteristics since the first contact during the expansionary period. However, the concentration on the study of ethnic groups in the region seems to have been overshadowed by curiosity and the potential interactions of various kinds, among Europeans, Africans and Arabs generated. Thus, this dissertation presents a study that aims to take a step toward filling this gap left open. To this end, the goal here is to contextualize the region during the sixteenth and seventeenth centuries and its place within the world of logic Overseas. With the proposed main promote an analysis of one of the best known ethnic groups period, mandingas. This proposal use the reports written three travelers Cape Verdeans who traded in the region of decades. Thus, we present here a reflective analysis on various matters pertaining to the theme of cultural studies, African history and images left to historians, through sources, the period of maritime expansion.

Dans cette thèse, une nouvelle loi d'endommagement en traction des roches sous chargement exploisif est établie. Basée sur l'approche micro-mécanique et énergétique, la loi d'évolution d'endommagement dynamique est conçue à l'aide de la méthode mathématique d'homogénéisation basée sur le développement asymptotique, et en tenant compte de l'effet inertiel lors de la propagation de fissure. Les simulations numériques sont présentées en vue d'illustrer la capacité du modèle à décrire les comportements connus comme les effets de taille pour la réponse structurelle, la sensibilité au taux de déformation, la transition fragile-ductile et la dispersion de l'onde.<p><p>La loi est implémentée dans le code aux éléments finis LAGAMINE pour étudier la réponse macroscopique du modèle. Plusieurs cas d'applications en dynamique ont été examinés.\\<p><p>- En 1D, les problèmes de localisation ont été étudiés dans une barre sollicitée en traction dynamique par une rampe de chargement. Selon l'amplitude de chargement, trois réponses ont été identifiées: a) comportement purement élastique pour des faibles charges, b) localisation à l'extrémité encastrée de la barre pour des chargements modérés et c) localisation à la tête de la barre pour des chargements élevés. L'influence de la taille microstrurelle sur la localisation a été examinée. Des simulations numériques de l'essai de traction dynamique par écaillage ont été éffectuées. Des essais expérimentaux de traction dynamique par écaillage ont été réalisés sur la bauxite et ont permis de valider le modèle en comparant l'analyse post mortem de l'éprouvette aux résultats des simulations numériques d'écaillage. Ces essais ont aussi permis de déterminer les caractéristiques mécaniques du matériau, la résistance mécanique en traction dynamique, l'instant de rupture et la vitesse de déformation à rupture.\\<p><p>- En 2D, des simulations numériques sont efféctuées pour reproduire le comportement d'une mine sous charge explosive. Le modèle a été capable de reproduire l'endommagement en traction sous charge explosive. L'influence des paramètres du modèle tels que: l'orientation de fissures, la taille de microstructure et la valeur initial d'endommagement sur la distribution de l'endommagement autour du trou de charge a été étudiée. Enfin, un cas d'application sur les mines de bauxite de Guinée a été étudié, incluant un calcul de l'extension de la zone endommagée ainsi qu'une prédiction numérique du niveau de vibration engendrée suite aux tirs à l'explosif. La prédiction du modèle est globalement en accord avec les résultats de la littérature. / Doctorat en Sciences de l'ingénieur / info:eu-repo/semantics/nonPublished

Trends towards ‘informalization’ are looming large in the world today. African cities have long been characterised by the presence of an ‘informal sector’ but are now experiencing new waves of ‘informalization’. Policies of liberalisation and structural adjustment are both changing the conditions under which urban dwellers make a living and encouraging states to abdicate from responsibilities for popular welfare. In this context, urbanites increasingly rely on informal ways of income earning and of social security provisioning. This book is about processes of ‘informalization’ in the West African city of Bissau in Guinea-Bissau. It begins with a historical account of the way conditions of informality have evolved through the encounter of locally specific forms of informal relations with colonialism and the socialist era. This is followed by an analysis of how disadvantaged groups who rely on informal ways of provisioning are faring in the context of contemporary changes. The study looks at both the informal income-generating activities and the social networks that urbanites engage in to sustain their income activities and their consumption. It seeks to assess whether these groups are coping with these wider changes or are becoming marginalised from networks of assistance and from activities that provide sufficient incomes. The social relations pervading access to support and livelihood resources as well as the informal rules governing such access are in focus. Forms of regulation in the informal sphere are also discussed. / <p>This thesis won the prize of “Best doctoral thesis in the Social Sciences at Stockholm University in 2001-2002”. Författaren är numera verksam vid Nordiska Afrikainstitutet</p>

The aim of the present investigation was to study the influence of the sympathetic nervous system and of various adrenoceptor agents on enzyme secretion and morphology in rat parotid and guinea-pig submandibular glands. Biochemical methods were combined with electron microscopical techniques. Two different in vitro systems were employed, batch-incubation and microperifusion, to characterize the sympathetically evoked amylase release and its correlation to cyclic AMP. By using various selective β-adrenoceptor agonists and antagonists a dominance of the β1-adrenoceptor over the β2 - in regulating amylase release - was establ ished. Continuous noradrenaline perifusion caused a rapid initial amylase discharge, closely correlated to tissue levels of cyclic AMP; no correlation between the two was observed during the later phase. Prenalterol (a β1-agonist) failed to elevate glandular cyclic AMP. This was in contrast to its potent secretagogic effect. On the other hand, terbutaline (a β2-agonist) was a weak secretagogue but markedly raised the levels of cyclic AMP. Thus, β-adrenoceptor activation may lead to release of large amounts of amylase despite minimal or no increase in cyclic AMP. Moreover, these effects seemed to be dissociated in salivary glands with regard to the β-adrenoceptor subtypes. This was further substantiated by the findings that repeated injections of prenalterol induced qualitative changes in the granule populations, similar to those caused by the non-selective β-agonist isoprenaline. Terbutaline was without effect. However, acinar cells size was increased following both prenalterol and terbutaline treatment. The data suggest that the 3-adrenergic effects on acinar cell size and granule population may be independently regulated. A decreased sympathetic activity of long duration was induced by neonatal or adult extirpation of the superior cervical ganlion on one side. Acinar cell size, as well as granule and amylase content was reduced 9 weeks after neonatal denervation. Ganglionectomy performed in adult animals was without significant effects. The secretory behaviour of neonatally denervated glands was characterized by an increased postjunctional sensitivity to 3-adrenoceptor agonists. Of special interest was the finding that neonatal denervation seemed to transform terbutaline from a partial to a full secretory agonist, thus changing its effects in the direction of those of prenalterol and noradrenaline. Moreover, increased levels of cyclic AMP as well as an enhanced response to DBcAMP were noted in the denervated glands as were intracellular changes. The denervation supersensitivity after neonatal denervation seems to differ from that observed in adult denervated glands. The results of the studies on denervated glands suggest that the sympathetic nervous system plays a fundamental role in the early maturation of the rat parotid gland as well as for the development of the β-adrenoceptor subtypes. / <p>S. 1-34: sammanfattning, s. 35-128: 6 uppsatser</p> / digitalisering@umu

Le golfe de Guinée constitue le principal enjeu de la géostratégie africaine post-bipolaire des Etats-Unis. Et pour cause, la région possède plusieurs forces profondes. Elles sont perceptibles avec les hydrocarbures liquides, devenus stratégiques pour les chefs d’état-major américains depuis qu’ils sont convoités, depuis 2000, par les Officiels chinois. Il ne s’agit donc pas pour les Etats-Unis d’accéder aux hydrocarbures de la région dans la seule finalité de diversifier leurs sources d’approvisionnement. Il s’agit avant tout de puissance. Pour les Officiels américains, il est vital d’endiguer dès à présent, et comme pour le Communisme hier, l’influence du « péril jaune » dans cette région stratégique. En réalité, l’ambition est, à terme, d’avoir à eux seuls, via leurs compagnies pétrolières, le « contrôle » exclusif du golfe de Guinée, ce qui ajouterait une valeur à leur puissance. Ces calculs répondent ainsi à l’impératif que poursuit leur politique étrangère post-bipolaire en général et post-11septembre 2001 en particulier : à savoir la pérennisation de l’hégémonie américaine par le « contrôle » des espaces-enjeux. L’autre force profonde de la région repose sur l’insécurité de la période allant de 1993 à 2008. Elle est liée à la mauvaise gouvernance de la rente pétrolière et des autres ressources stratégiques de la région. De même, le retard des Etats de la région dans les processus de démocratisation, la piraterie maritime qui sévit sur les côtes ouest africaines, sont autant de menaces et d’incertitudes qui pèsent sur la région. Elles préoccupent les administrations Clinton et Bush car elles sont susceptibles d’hypothéquer les intérêts vitaux des Etats-Unis dans la région. / The gulf of Guinea has being the main concern of the United States foreign policy toward Africa since the end of the Cold War. The reason can be found in the region’s fundamental forces, an instance by no means least being oil. The oil deposits of the gulf of Guinea have become strategic for U.S. Officials since Chinese Officials started coveting them. The access to the oil of the region is not for them mainly a way of diversifying their supply sources, but rather a matter of keeping up their hegemony. Indeed, as the United States did yesterday with Communism, they do today with China containing its influence in this region of greater importance. Their ambition is to gain the exclusive « control » over the gulf of Guinea via their oil companies. This strategy meets their post-Cold War foreign policy, and aims at perpetuating the American hegemony through the control of regions of greater importance. This policy has been re-enforced since September, 11, 2001. Other fundamental forces include bad governance of incomes from strategic resources such as oil. Other uncertainties that weigh on the gulf of Guinea include the sluggishness of the region in setting in place democratic processes, and sea piracy. All of them have worried both Clinton and Bush’s administrations: there are threats to US “vital” interests in the region.