Cyanobacteria (blue-green algae) produce a diverse array of toxic or otherwise bioactive metabolites. These allelochemicals may also play a role in defense against potential predators and grazers, particularly aquatic invertebrates and their larvae, including mosquitoes. Compounds derived from cyanobacteria collected from the Florida Everglades and other Florida waterways were investigated as insecticides against the mosquito Aedes aegypti, a vector of dengue and yellow fever. Screening of cyanobacterial biomass revealed several strains that exhibited mosquito larvicidal activity. Guided via bioassay guided fractionation, a non-polar compound from Leptolyngbya sp. 21-9-3 was found to be the most active component. Characterization revealed the prospective compound to be a monounsaturated fatty acid with the molecular formula C16H30O2. This is the first evidence of mosquito larvicidal activity for this particular fatty acid. With larvicidal becoming more prevalent, fatty acids should be explored for future mosquito control strategies.

Bacillus-derived larvicides, which selectively target mosquito (Diptera: Nematocera: Culicidae) populations to reduce nuisance and health risks, were applied in the South March Highlands Conservation Forest near residential neighbourhoods in Ottawa, Ontario. The objective was to assess effects of application on the nontarget mosquito relative, Chironomidae (Diptera: Nematocera: Chironomidae), and other nontarget aquatic taxa captured using emergence traps. A secondary objective was to assess physicochemical variables that influence Chironomidae emergence. Study ponds received an application of Bacillus thuringiensis var. israeliensis, a subset also received an application of Bacillus sphaericus, and a group of control ponds were left untreated over 3 years (2016-2018). Weekly sampling included trap collections and measurements of water temperature, pH, water depth, conductivity, dissolved oxygen, ammonia, nitrate, and sulphate. Drought in 2016, high precipitation throughout 2017, and seasonal precipitation in 2018 influenced variable physicochemical conditions. Principal component analyses identified differences between sampling groups and between years. Redundancy analyses correlated insect emergence with pond pH, average water depth and water temperature and indicated a strong relationship between Chironomidae emergence and average water depth. Although significantly less Chironomidae annual emergence was observed at treated sites in 2017 and 2018, zero-inflated negative binomial generalized linear mixed modelling failed to detect a significant Bti treatment effect when controlling for within group variation. Rather, variations in pH, mean water depth and water temperature were identified as drivers of Chironomidae emergence. Culicidae emergence was reduced to zero briefly following treatment in 2017 and 2018. The model detected a marginal negative treatment effect on Culicidae in 2017 only, and a positive treatment effect in 2018 at the onset of a secondary hydroperiod, in the absence of treatment. Variations in pH and water temperature were also identified to be drivers of Culicidae emergence. Modelling failed to detect treatment effects on any of the nontarget taxa abundance, including Diptera, Lepidoptera, Ephemeroptera, Odonata, Coleoptera, Hymenoptera, and Arachnida. An inverse relationship between insectivore and prey taxa abundance was observed. In 2018, taxa richness increased between years and trended higher at treated sites and a positive relationship between insectivore and prey taxa richness was observed. In 2017, Shannon-Weiner index and Simpson’s index of diversity were higher at untreated sites, and in 2018 diversity indices were higher at treated sites, with taxa richness increasing between years and higher evenness trending at treated sites. Our data suggest that treatment effects were potentially shrouded by natural variability of physicochemical variables, especially due to the varying hydroperiod observed over the three years of sampling. Additional work is needed to capture average conditions and separate confounding variables from treatment effects. This study provides an inventory of the current wetland insect community in the South March Highlands Conservation Forest landscape that offers a reference for ongoing mosquito management.

Submitted by hugo lira (hugovictorsb@gmail.com) on 2018-09-03T13:11:08Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 1. Tese de Doutorado - Grafe Pontes.pdf: 3805661 bytes, checksum: dfa0075a7b7b500d45f3604fdd0ab8b0 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-09-10T15:39:31Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 1. Tese de Doutorado - Grafe Pontes.pdf: 3805661 bytes, checksum: dfa0075a7b7b500d45f3604fdd0ab8b0 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-09-10T15:49:55Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 1. Tese de Doutorado - Grafe Pontes.pdf: 3805661 bytes, checksum: dfa0075a7b7b500d45f3604fdd0ab8b0 (MD5) / Made available in DSpace on 2018-09-10T15:49:55Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) 1. Tese de Doutorado - Grafe Pontes.pdf: 3805661 bytes, checksum: dfa0075a7b7b500d45f3604fdd0ab8b0 (MD5) Previous issue date: 2018-05-02 / FAPEAM - Fundação de Amparo à Pesquisa do Estado do Amazonas / The use of chemical insecticides becomes important in Aedes aegypti control programs. Over the years, epidemiological studies have demonstrated the inefficiency of synthetic insecticides due to the resistance acquired with the use in activities related to vector control in tropical diseases, especially dengue. For a better effectiveness of vector control programs, the search for new alternatives is necessary. The interest in entomopathogenic fungi for biological control is in great growth. However, the work on controlling the different stages of life of A. aegypti and the transmission of mycoses between individuals and their offspring are still discrete. In order to contribute with new compounds and integrated control of dengue, the present project aims to evaluate the entomopathogenic activity of fungi isolated from Amazonian soil in larvae and adults of A. aegypti, and their residual effects on the life cycle considering the number of eggs and larvae and adults. The proposed methodology for this work is the use of conidia of fungal isolates previously selected by genera that have relevance in biological control or that demonstrate positive results when used in probe / sorting insects, Tenebrio molitor. For the application of the formulations conidia were used in aqueous suspensions for treatment in larvae, and for adults was used aqueous suspensions impregnated in filter paper placed in Kit of the World Health Organization used to evaluate the susceptibility of chemical insecticide. The present work proposes a practical methodology for sexing and separating adults through the selection of pupae, in order to evaluate the degree of infestation and the entomopathogenic effect on larvae and adults of A. aegypti. avoiding the premature death of the insect by the use of Castro's catcher at the time of adult withdrawal from the colony. The results obtained evidenced the potential of four fungal isolates IPC 2.1 and IPS 3.1 isolated from Amazonian soil, and two other isolates from other insects and from soil samples IBCB 66 and IBCB 425, widely used to control insects in agriculture, and evaluated for the control of larvae and adults of the mosquito vector dengue. The results between the two methodologies showed the best applicability for the fungal isolates and their use in the control of A. aegypti, responsible for a great social impact on the public health of the Amazon due to the transmission of several arboviruses. / O uso de inseticidas químicos tornou importante nos programas de controle do Aedes aegypti. Com o passar dos anos, os estudos epidemiológicos demonstraram a ineficiência dos inseticidas sintéticos devido à resistência adquirida com a utilização nas atividades relacionadas ao controle de vetores em doenças tropicais, especialmente dengue. Para uma melhor eficácia dos programas de controle de vetores, há necessidade da busca de novas alternativas. O interesse em fungos entomopatogênicos para controle biológico encontra-se em amplo crescimento. Entretanto, ainda são discretos os trabalhos no controle dos diferentes estágios de vida do A. aegypti, e a transmissão de micoses entre seus indivíduos e sua prole. Com o intuito de contribuir com novos compostos e no controle integrado da dengue, Febre Chikungunya e Zika vírus. O presente projeto tem como objetivo avaliar a atividade entomopatogênica de fungos isolados de solo da Amazônia em larvas, adultos de A. aegypti, e seus efeitos residual sobre o ciclo de vida considerando o número de ovos e larvas e adultos. A metodologia proposta para esse trabalho visa à utilização de conídios dos isolados fúngicos previamente selecionados por gêneros que tenham relevância no controle biológico ou que demonstrem resultados positivos quando utilizados em insetos sonda/triagem, Tenebrio molitor. Para a aplicação das formulações foram utilizados conídios em suspensões aquosas para tratamento em larvas, e para adultos foi utilizado suspensões aquosas impregnados em papel filtro colocados em Kit da Organização Mundial de Saúde utilizado para avaliação de susceptibilidade de inseticida químico. Os bioensaios foram realizados em condições de laboratório, avaliando-se o grau de infestação e o efeito entomopatogênico sobre as larvas e adultos de A. aegypti, O presente trabalho propõe uma metodologia prática para sexagem e separação de adultos por meio de seleção de pupas, evitando a morte prematura do inseto pelo uso de capturador de Castro no momento de retirada de adultos da colônia. Os resultados obtidos evidenciaram o potencial de quatro isolados fúngicos, IPC 2.1 e IPS 3.1 isolados de solo da Amazônia, e outros dois isolados de outros insetos e de amostras de solo, IBCB 66 e IBCB 425, amplamente utilizados para controle de insetos na agricultura, e avaliados para combate de larvas e adultos do mosquito vetor da dengue, Febre Chikungunya e Zika vírus. Os resultados entre as duas metodologias demonstraram a melhor aplicabilidade para os isolados fúngicos e sua utilização no controle do A. aegypti, responsáveis por grande impacto social na saúde pública da Amazônia devido a transmissão de diversas arboviroses.

CoordenaÃÃo de AperfeÃoamento de Pessoal de NÃvel Superior / Os Ãleos essenciais produzidos por diversas espÃcies vegetais sÃo misturas, por vezes, bastante complexas com variadas funÃÃes ecolÃgicas como inibiÃÃo de germinaÃÃo, proteÃÃo contra predadores, atraÃÃo de polinizadores, proteÃÃo contra perda de Ãgua e aumento da temperatura. Os Ãleos essenciais sÃo obtidos atravÃs de mÃtodos fÃsicos de extraÃÃo e apresentam diversas aplicaÃÃes, como flavorizantes, conservantes, fixadores, agentes antibacterianos e antifÃngicos. VÃrios produtos industriais tambÃm utilizam Ãleos essenciais em suas formulaÃÃes, como pesticidas, repelentes e atraentes se apresentando como uma alternativa natural no controle ou na eliminaÃÃo de pragas. A restriÃÃo de alguns pesticidas sintÃticos promoveu ainda mais a pesquisa em busca de novos produtos que pudessem desempenhar a funÃÃo daqueles que nÃo estÃo mais disponÃveis no mercado. VÃrios biopesticidas estÃo relatados na literatura e dentre eles o dilapiol, que à um fenilpropanoide de atividade inseticida comprovada, encontrado em alto teor no Ãleo essencial de Piper aduncum L. (Piperaceae). Neste trabalho, foi analisada a variabilidade quantitativa do dilapiol nos Ãleos essenciais (OE) obtidos das folhas frescas de P. aduncum e quando submetidas a 24, 48, 72 e 96h de secagem a 40 ÂC. Os OE foram submetidos a reaÃÃes de hidrogenaÃÃo e hidroxilaÃÃo em busca de biopesticidas mais ativos e todos os produtos foram analisados por CG-EM, RMN1H e RMN13C. O teor de dilapiol foi analisado por CGâFID e por CLAE, obtendo-se em mÃdia 89,68% e 89,91% respectivamente, e permitiu concluir que a temperatura de secagem nÃo influencia no conteÃdo deste composto presente no OE de P. aduncum. O segundo componente majoritÃrio do Ãleo foi identificado como β-cariofileno (4,47%). O ensaio larvicida frente a Aedes aegypti realizado forneceu IC50 de 47,12 mg/L, confirmando o potencial deste produto como biopesticida. / Essential oils produced by various plant species are mixtures sometimes quite complex with varied ecological functions, such as inhibition of germination, protection against predators, attraction of pollinators, protection against water loss and temperature rise. Essential oils are obtained by physical extraction methods and present several applications, such as flavorings, preservatives, fixatives, antibacterial and antifungal agents. Various industrial products also use essential oils in their formulations, such as pesticide repellents and attractive presenting as a natural alternative to control or eliminate pests. The restriction of some synthetic pesticides further promoted the search for new products that could play the role of those who are no longer available in the market. Several biopesticides are reported and among them dilapiolle, which is a phenylpropanoid with proven insecticidal activity found in high content in essential oil of Piper aduncum L. (Piperaceae). In this work, we analyzed the quantitative variability in dilapiolle essential oils (EO) obtained from fresh leaves of P. aduncum and when subjected to 24, 48, 72 and 96 h of drying at 40 ÂC. The EO underwent hydrogenation and hydroxylation reactions in search of more active biopesticides and all products were analyzed by GC-MS, NMR 1H and NMR 13C. The content of dilapiolle was analyzed by GC-FID and HPLC, yielding on average 89.68 % and 89.91 % respectively and showed that the drying temperature does not influence the content of this compound in P. aduncum EO. The second major component of the oil was identified as β-caryophyllene (4.47 %). The test larvicide against Aedes aegypti performed provided IC50 of 47.12 mg/L, confirming the potential of this product as a biopesticide.

Submitted by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-10-18T14:39:20Z No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) TeseParcial(Cap.I)-RaimundoJunior-PPGQ.pdf: 1178770 bytes, checksum: 74333ca704cd865ec51c2e655679a37f (MD5) Reprodução Não Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) / Approved for entry into archive by Divisão de Documentação/BC Biblioteca Central (ddbc@ufam.edu.br) on 2018-10-18T14:39:33Z (GMT) No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) TeseParcial(Cap.I)-RaimundoJunior-PPGQ.pdf: 1178770 bytes, checksum: 74333ca704cd865ec51c2e655679a37f (MD5) Reprodução Não Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) / Made available in DSpace on 2018-10-18T14:39:33Z (GMT). No. of bitstreams: 3 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) TeseParcial(Cap.I)-RaimundoJunior-PPGQ.pdf: 1178770 bytes, checksum: 74333ca704cd865ec51c2e655679a37f (MD5) Reprodução Não Autorizada.pdf: 47716 bytes, checksum: 0353d988c60b584cfc9978721c498a11 (MD5) Previous issue date: 2018-05-28 / The Myrtaceae family has 132 genera distributed in 5,760 species present in Australia, Southeast Asia, tropical and temperate America. In Brazil, it presents 1,018 species in 23 genera. Myrcia D.C is one of the largest genera with 282 species present in almost all territory, of which 220 are endemics. Its species are used in Brazilian popular medicine, with emphasis on Myrcia spp., which are used by the traditional population of the Amazon as astringents, diuretics, hypoglycemics, antihemorrhagics, antioxidants and in the treatment of hypertension and ulcers. The present study has evaluated the chemical composition of the essential oils of 13 species of Myrtaceae (Myrcia spp., Marlierea sp. and Calyptranthes sp.) from the Terra Firme ecosystem (Amazon). In addition, the cytotoxic, antibacterial, bacteriostatic, antifungal and larvicidal potentials of these oils have been described. Twenty-seven samples belonging to these thirteen species (18 individuals of 11 species of Myrcia, one of Marlierea caudata and one of Calyptranthes spruceana) were collected from the Adolpho Ducke Forest Reserve, EMBRAPA (Manaus) and the PANC site (Manaus). Its essential oils were obtained by hydrodistillation, dried and stored under refrigeration. The chemical characterization was carried out by means of CG-DIC and CG-EM analysis. The Arithmetic Indexes and the Spectral Similarity Indexes were obtained, whose results were compared with those described in the main databases. The mean chemical characterization of these oils was 95%, totaling 336 compounds identified, of which 34 have been abundant (50.7 to 96.17%). The most frequent and, in some cases, the most frequent components are: (E)-caryophyllene, δ-cadinene, Espatulenol, α-Copaene, β-elemene, α-humulene, Caryophyllene oxide, β-selinene, α-muurolene and α–cadinol (42.73% of nonoxygenated sesquiterpenes and 36.98% of oxygenated sesquiterpenes). This chemical variability is common to Myrcia species of other Brazilian ecosystems/biomes. Of the seven pairs of Myrcia individuals collected in distinct periods (dry and rainy), 242 compounds were identified, of which 125 were common, 41 and 76 of which were unique to the rainy and dry periods, respectively. Essential oils of the dry period have exhibit greater chemical variability. In addition, the chemical variability of the essential oils of Myrcia spp. of this ecosystem has been described by 51 cyclization pathways. Of these routes, thirteen have a high frequency (above 50%), four of which are the most prominent ones (Cariofilan, Cadinano, Aromadendrano and Eudesmano). The chemical results of this study have revealed a clear agreement of the chemical composition of Marlierea caudata with the chemistry composition of Myrcia spp.. On the other hand, this observation is not clearly evident for C. spruceana. Moreover, It should be noted that the chemical composition of the essential oils of M. magnoliifolia, M. minutiflora, M. fenestrata, M. amapensis and Marlierea caudata have been described for the first time. As for toxicity to cancer cells (Skmel 3 and ACPO2) and normal cells (MRC5), M. minutiflora presents moderate activity for human melanoma (Skmel 3) and gastric adenocarcinoma (ACPO2) as well as cytotoxic to non-neoplastic fibroblasts (MRC5). M. citrifolia, M. minutiflora, M. paivae and M. magnoliifolia present moderate to high activity against Staphylococcus aureus. M. fallax, M. sylvatica, M. paivae and C. spruceana present moderate activity against Staphylococcus aureus. As for the bacteriostatic action, all species tested have shown moderate to high activity against Pseudomonas aeroginosas. In relation to the insecticidal activity, M. citrifolia, M. bracteata, M. fenestrata, M. amazonica, M. paivae and C. spruceana are active, since after 24 h the mortality percentage of Aedes aegypti larvae was 100% in 25 mg.L- 1 of essential oil. Furthermore, the use of chemometric tools in this study has shown it possible to observe the segregation of samples by major terpene groups and their most influential cyclic pathways, whose substances of major relevance in this model have been (E)-Caryophyllene, δ-Cadinene, and Espatulenol. Its pharmacological activities described in the literature should suggest that they are responsible for the biological results observed in this work. However, novel biological assays with such isolated constituents must be necessary. Therefore, with respect to the chemistry of Myrcia volatile constituents occurring in Terra Firme (Amazonia), the present study has been evidenced very characteristic Amazonian chemotypes and without similarity with other species collected outside the region, which leads us to conclude how much to must be studied from the precious Amazonian biodiversity. / A família Myrtaceae apresenta 132 gêneros distribuídas em 5.760 espécies presentes na Austrália, sudeste da Ásia, América tropical e temperada. No Brasil, apresenta-se com 1018 espécies em 23 gêneros. Myrcia D.C é um dos maiores gêneros com 282 espécies presentes em quase todo território, sendo 220 endêmicas. Suas espécies são empregada na medicina popular brasileira, com destaque para Myrcia spp., as quais são usadas pela população tradicional da Amazônia como adstringentes, diuréticos, hipoglicemicas, anti-hemorrágicas, antioxidantes e no tratamento de hipertensão e úlceras. O presente estudo avalia a composição química dos óleos essenciais de 13 espécies de Myrtaceae (Myrcia spp., Marlierea sp. e Myrciaria sp.) de ecossistema de Terra Firme (Amazônia). Além disso são descritos os potenciais citotóxico, antibacteriano, bacteriostático, antifúngico e larvicida desses óleos. Vinte sete amostras pertencentes a essas treze espécies (18 indivíduos de 11 espécies de Mycia, um indivíduo de Marlierea caudata e outro de Calyptranthes spruceana) foram coletados na Reserva Florestal Adolpho Ducke, na EMBRAPA (Manaus) e no sítio PANC (Manaus). Seus óleos essências foram obtidos por hidrodestilação, secos e armazenado sob refrigeração. A caracterização química ocorreu por meio de análises por CG-DIC e CG-EM, cujos Índices Aritiméticos calculados e de Similaridade Espectral foram comparados com os descritos nas principais bases de dados. A média de caracterização química desses óleos foi de 95%, totalizando 336 substâncias identificadas, das quais 34 são abundantes (50,7 a 96,17%). Os componentes mais frequentes, e em alguns casos majoritários, são: (E)–cariofileno, δ–cadineno, Espatulenol, α–copaeno, β–elemeno, α–humuleno, Óxido de cariofileno, β–selineno, α–muuroleno e α–cadinol, prevalecendo estruturas sesquiterpênicas (42,73% de sesquiterpenos não-oxigenados e 36,98% de sesquiterpenos oxigenados). Essa variabilidade química é comum a espécies de Myrcia de outros ecossistemas/biomas brasileiros. Dos sete pares de indivíduos de Myrcia coletados em períodos distintos (seco e chuvoso), identificou-se 242 substâncias, sendo 125 comuns, com 41 exclusivas do período chuvoso e 76 são exclusivos do períoso seco. Os óleos essenciais do período seco apresentaram uma maior variabilidade química. Além disso, a variabilidade química dos óleos essenciais de Myrcia spp. desse ecossistema pôde ser descrita por 51 vias de ciclização. Dessas vias, treze apresentam elevada frequência (superior a 50%), sendo quatro vias de destaque pela maior ocorrência (Cariofilano, Cadinano, Aromadendrano e Eudesmano). Os resultados químicos desse estudo revelam uma clara concordância de Marlierea caudata com a química de Myrcia spp., porém o mesmo não se evidencia claramente para C. spruceana. Ressalta-se que a composição química dos óleos essenias de M. magnoliifolia, M. minutiflora, M. fenestrata, M. amapensis e Marlierea caudata são descritos pela primeira vez. Quanto à toxicidade frente a células cancerígenas (Skmel 3 e ACPO2) e normais (MRC5), M. minutiflora apresenta atividade moderada para melanoma humano (Skmel 3) e adenocarcinoma gástrico (ACPO2), bem como citotóxica para fibroblastos nãoneoplásicos (MRC5). M. amazonica e M. fenestrata também apresentam atividade moderada para Skmel 3. Quanto ao potencial antimicrobiano e bacteriostático, M. citrifolia, M. minutiflora, M. paivae e M. magnoliifolia apresentam atividade moderada a elevada frente a Staphylococcus aureus. M. fallax, M. sylvatica, M. paivae e C. spruceana apresentam atividade moderada frente a Staphylococcus aureus. Quanto a ação bacteriostática, todas as espécies ensaiadas apresentam atividade moderada a elevada frente a Pseudomonas aeroginosas. Em relação a atividade inseticida, M. citrifolia, M. bracteata, M. fenestrata, M. amazonica, M. paivae e C. spruceana são ativas, pois após 24 h o percentual de mortalidade das larvas de Aedes aegypti é de 100% em 25 mg.L-1 de óleo essencial. A aplicação de ferramentas quimiométricas nesse estudo possibilitou observar a segregação das amostras por grupos terpênicos majoritários e suas vias de ciclização mais influentes, cujas substâncias de maior relevância nesse modelo são: (E)-cariofileno, δ–cadineno e Espatulenol. Suas atividades farmacológicas descritas na literatura sugerem serem os responsáveis pelos resultados biológicos observados nesse trabalho. Contudo, novos ensaios biológicos com tais constituintes isolados talvez sejam necessários. Portanto, a respeito da química dos constituintes voláteis de Myrcia ocorrentes em Terra Firme (Amazônia), o presente estudo evidência quimiotipos amazônicos bem característicos e sem similaridade com outras espécies coletadas fora da região, o que nos leva a concluir o quanto ainda há a ser estudado a partir da preciosa biodiversidade amazônica.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Amburana cearensis (Allemao) A. C. Smith, usually known in portuguese language as âImburana-de-Cheiroâ or âCumaruâ, is a leguminous tree, subfamily Papilionoideae with common occurrence in âCaatingaâ Dominium. Despite the numerous ethnobotanical uses, few studies report biological activities of medical interest and / or agribusiness for this species or yet its utilization as food for humans and / or animals. This study aimed to evaluate the proteins of A. cearensis on its nutritional value associated with its biochemical characterization and as to the presence of bioactivity against bacteria, yeasts and filamentous fungi, all pathogens of humans, animals or plants of economic importance, and also against the vectors of disease, Aedes aegypti and Ae. albopictus. For this, seeds were collected in areas of caatinga trees in the municipality of Quixadà - CE and, subsequently, dried and finely ground. The seeds of A. cearensis have high nutritional potential, evidenced by the high content of proteins (22.69 &#61617; 0.81 g 100 g-1), lipid (24.45 2.02 g 100 g-1) and dietary fiber (34.75 1.78 g 100 g-1), keeping smaller quantities of starch (11.43 0.18 g 100 g-1), of total sugars (5.60 0.09 g 100 g-1) and minerals (4.51 0.21 g 100 g-1). Its amino acid composition is comparable to that of soybeans and beans. Moreover, the seeds have moderate levels of antinutritional factors, being detected only trypsin inhibitory activity (27.41  0.03 gTI Kg-1), urease activity (434  34 U kg-1) and some secondary metabolites such as tannins, phenols, flavones, flavonols, and xantone steroids. Chemical components, probably of low molecular mass present in seeds flour incorporated into diets, seem to interfere with their acceptance and use by animals since when the protein fraction (F0/90), obtained from its soluble protein, was incorporated into the balanced diet, the rats accepted well the diets and their performance indicated that F0/90 a good source of protein, comparable to major sources of vegetable protein such as soy and beans. Proteins of A. cearensis seeds are mainly composed of globulins (74.43 g 100 g-1) and albumins (14.23 g 100 g-1), with smaller quantities of glutelin basic proteins (10.07 g 100g-1), prolamins (1.20 g 100 g-1) and glutelin acidic proteins (0.07 g 100 g-1). The seeds crude extract (GE) offers a wide variety of proteins as shown by electrophoretic profiles with a predominance of proteins with apparent molecular mass over 45.0 kDa. The seeds of A. cearensis show also high bioactive potential, especially the activities of their majority proteins (albumins and globulins) against the growth of human pathogenic bacteria and yeast and phytopathogenic filamentous fungi, and against larvae of Aedes aegypti and Ae. albopictus. The inhibitory activity of the growth of phytopathogenic fungi concentrated in the albumins F70/90 fraction, being rich in proteins of low molecular mass (<30 kDa). Thus we can conclude that F0/90 is a good source of protein food when compared to other plant sources already used by the population. In addition, the globulins and albumins of A. cearensis are promising sources of bioactive proteins to be studied further and in greater purity. / Amburana cearensis (Allemao) A. C. Smith, popularmente conhecida como Imburana-de-Cheiro ou Cumaru, à uma leguminosa arbÃrea da subfamÃlia Papilionoideae de ocorrÃncia freqÃente na regiÃo do domÃnio Caatinga. Apesar dos inÃmeros usos etnobotÃnicos, poucos trabalhos relatam atividades biolÃgicas de interesse mÃdico e/ou agroindustrial para esta espÃcie ou, mesmo, sua utilizaÃÃo como fonte de alimento para humanos e/ou animais. Assim, o presente trabalho objetivou avaliar as proteÃnas de A. cearensis quanto a seu valor nutricional aliado à sua caracterizaÃÃo bioquÃmica e quanto à presenÃa de bioatividade contra bactÃrias, leveduras e fungos filamentosos, todos patÃgenos do homem, de animais ou de plantas de importÃncia econÃmica, e, ainda, contra os vetores de doenÃas, Aedes aegypti e Ae. albopictus. Para tanto, sementes foram coletadas em Ãrea de caatinga arbÃrea, no municÃpio de Quixadà â CE, sendo, posteriormente, desidratadas e finamente moÃdas. As sementes de A. cearensis possuem alto potencial nutricional, revelado pelo alto teor de proteÃnas (22,69 &#61617; 0,81 g. 100 g-1), lipÃdios (24,45 2,02 g. 100 g-1) e fibra alimentrar (34,75 1,78 g.100 g-1), retendo quantidades menores de amido (11,43 0,18 g.100g-1), aÃÃcares totais (5,60 0,09 g.100 g-1), e de minerais (4,51 0,21 g.100 g-1). Sua composiÃÃo aminoacÃdica à comparÃvel Ãquela da soja e feijÃo. AlÃm disso, as sementes apresentam moderados nÃveis de fatores antinutricionais, sendo detectados apenas atividade inibitÃria de tripsina (27,41  0,03 gTI.Kg-1), atividade ureÃsica (434,0  34,0 U.Kg-1) e alguns metabÃlitos secundÃrios, como taninos, fenÃis, flavonas, flavonÃis, xantonas e esterÃides. Componentes quÃmicos, provavelmente de baixa massa molecular, presentes nas sementes incorporadas a dietas interferem na sua aceitaÃÃo e aproveitamento por parte dos animais, uma vez que quando a fraÃÃo proteica (F0/90) obtida de suas proteÃnas solÃveis à incorporada à dieta balanceada, apresenta boa aceitaÃÃo pelos animais, mostrando-se uma boa fonte de proteÃnas, comparÃvel a importantes fontes de proteÃnas vegetais como a soja e os feijÃes. As proteÃnas das sementes de A. cearensis sÃo compostas principalmente por globulinas (74,43 g. 100g-1) e albuminas (14,23 g.100 g-1), com quantidades menores de proteÃnas do tipo glutelinas bÃsicas (10,07 g.100 g-1), prolaminas (1,20 g.100 g-1) e proteÃnas do tipo glutelinas Ãcidas (0,07 g.100 g-1). O extrato bruto (EB) das sementes apresenta uma grande diversidade de bandas proteicas com predominÃncia de bandas com massa molecular aparente > 45,0 kDa. As sementes de A. cearensis possuem tambÃm alto potencial bioativo, destacando-se as atividades de suas proteÃnas majoritÃrias (globulinas e albuminas) contra o crescimento de bactÃrias e leveduras patÃgenas do homem e de fungos filamentosos fitopatogÃnicos, e atividade contra larvas de Aedes aegypti e Ae. albopictus. A atividade inibitÃria do crescimento de fungos fitopatogÃnicos concentra-se na fraÃÃo F70/90 das albuminas, sendo rica em proteÃnas de baixa massa molecular (< 30 kDa). Assim, pode-se concluir que a F0/90 à uma boa fonte de proteÃna alimentar quando comparada a outras fontes vegetais jà utilizadas pela populaÃÃo. AlÃm disso, as globulinas e albuminas de A. cearensis sÃo fontes promissoras de proteÃnas bioativas que devem ser estudadas mais detalhadamente e em maior grau de pureza.