The aim of this study was to determine the species boundaries within the Aspergillus viridinutans species complex. The species belonging to this complex are predominantly soil- inhabiting organisms that are increasingly reported as opportunistic human and animal pathogens. A total number of 98 isolates from various substrates and countries was subjected to morphological, physiological and molecular analysis (calmodulin, β-tubulin, actin and RNA polymerase II subunit 2 gene) and mating experiments were provided on different media and temperatures. Some other heterothallic species from section Fumigati with unknown sexual state were analysed using similar method as well. Key words: Aspergillus viridinutans, Aspergillus turcosus, phylogenetic analysis, mating- type genes, anamorph, teleomorph, mating experiments, MAT1-1, MAT1-2

Pyrenophora semeniperda is a generalist fungal pathogen that occurs primarily on monocot seed hosts. It is in the phylum Ascomycota, which includes both self-compatible (homothallic) and self-incompatible (heterothallic) species. Homothallic fungal species contain complementary mating-type (MAT) idiomorphs in a single unikaryotic strain, while heterothallic strains contain a single MAT idiomorph requiring interaction between strains of complementary mating-types for sexual reproduction to occur. Because the majority of P. semeniperda strains contained either MAT1 or MAT2, this species was provisionally categorized as heterothallic. However, many strains contain both MAT idiomorphs and appear to be homothallic. These results warranted a closer look at the MAT idiomorphs and the structure of the P. semeniperda genome in order to assure accurate characterization of the MAT locus. Additionally, an assessment of the geographic distribution of MAT idiomorphs provides us with insight into the genetic diversity of P. semeniperda and the reproductive strategies that it employs. In this study, we characterized the P. semeniperda MAT locus and assessed the idiomorph distribution of 514 isolates from 25 P. semeniperda populations collected from infected Bromus tectorum (cheatgrass) seeds. Additionally, we used simple sequence repeat (SSR) and MAT idiomorph length polymorphisms to demonstrate the existence of dikaryotic strains and pseudohomothallism in this fungus. We identified a unique variable number tandem repeat (VNTR) within each idiomorph of the MAT locus of P. semeniperda. Presence of the VNTR in all MAT loci analyzed from strains collected in the Intermountain West suggests ancient proliferation of this repeat. The persistence and effectiveness of P. semeniperda strains in the cheatgrass pathosystem depend not only on the density of the fungus in the soil, but also on the genetic heterogeneity of each population. Our study suggests that P. semeniperda genetic diversity is increased both through MAT locus-dependent sexual reproduction and asexually through anastomosis.

Syftet med uppsatsen är att undersöka hur mediers gestaltning av mat och matlagning reproducerar livsstilar och identiteter. De senaste åren har mat börjat förekomma alltmer frekvent i olika medier. Varje dag släpps en ny bok om matlagning i Sverige, när vi bläddrar bland kanalerna på tv:n visas nästan alltid någon form av matlagningsprogram och i affären syns rader av matmagasin i tidningsställen. I genomförandet av vår undersökning har vi valt att använda oss av identifikationsteorin och teorin om användarperspektivet. För att göra detta har vi gjort en kvalitativ textanalys av artiklar ur två svenska tidningar, Dagens Nyheter och Allt om mat. Med hjälp av ett kodschema har varje artikel analyserats på både makro- och mikronivå. För att få en historisk översikt och landa så nära nutid som möjligt har vi gjort nedslag i 1973, 1993 och 2013. Resultatet av vår studie visar att det har skett en förändring i matjournalistiken. De tidigare nedslagen var det maten som stod i fokus. Men mellan 1993 och 2013 har det skett en förskjutning i fokus. Det är människorna bakom maten som får störst utrymme i artiklarna. Möjligheterna till identifikation är mycket större 2013 än var de var 1973 och 1993.

EXPLORING THE POTENTIAL OF MAT-BUILDING FOR THE CREATION OF UNIVERSALLY DESIGNED ENVIRONMENTS Eren, Yasemin M. Arch., Department of Architecture Supervisor: Assoc. Prof. Dr. Emel Ak&ouml / zer Co-Supervisor: Assoc. Prof. Dr. Halime Demirkan June 2004, 152 Pages The goal of this study is to reread the strategies of formal organization in architecture, which are called &ldquo / mat-building&rdquo / and &ldquo / mat-urbanism&rdquo / , exploring their potential to contribute to the creation of universally designed built environments in the 21st century. The idea of mat-building was first delineated by Alison Smithson in 1974, in her article &ldquo / How to Recognize and Read Mat-Building,&rdquo / by means of its traditional and modern examples. The concept of universal design was first used in 1970&rsquo / s and reinterpreted by the American architect Ronald Mace in 1985. Since then, it has become a widely accepted design approach that is also known as &lsquo / inclusive design&rsquo / and &lsquo / design for all&rsquo / . Mat-building can be considered as a viable design approach that can respond to the crucial need for equally accessible, adjustable and adaptable built environments for all people all over the world. The study aims not only to evaluate the exemplary mat like configurations in light of the universal design principles. It also tries to point to the new ways for developing creative ideas and design theories, and emphasizes the significance of implementing the universal design approach in contemporary architecture and urbanism.

I mitt projekt har jag hängivit mig åt leken. Använt dess kraft och engagemang för att bryta gränser, normer och hitta nya lekfulla arbetsmetoder.  Jag frågar mig själv, hur påverkar leken mig som designer och vilka produkter ger den upphov till? Genom lekfullhet, mat och musik vill jag skapa upplevelser som stimulerar våra sinnen och smaklökar.

Submitted by Ana Maria Fiscina Sampaio (fiscina@bahia.fiocruz.br) on 2015-09-29T16:45:14Z No. of bitstreams: 1 Alcinéia Oliveira Damiao caracterização ....PDF.pdf: 1250268 bytes, checksum: 8e253d5ec93aa5123f3f85b88d8bc154 (MD5) / Approved for entry into archive by Ana Maria Fiscina Sampaio (fiscina@bahia.fiocruz.br) on 2015-09-29T16:45:29Z (GMT) No. of bitstreams: 1 Alcinéia Oliveira Damiao caracterização ....PDF.pdf: 1250268 bytes, checksum: 8e253d5ec93aa5123f3f85b88d8bc154 (MD5) / Made available in DSpace on 2015-09-29T16:45:29Z (GMT). No. of bitstreams: 1 Alcinéia Oliveira Damiao caracterização ....PDF.pdf: 1250268 bytes, checksum: 8e253d5ec93aa5123f3f85b88d8bc154 (MD5) Previous issue date: 2015 / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / A leptospirose é uma zoonose de distribuição mundial, com 1,2 milhões de casos registrados a cada ano. De 1996 a 2013, o grupo de pesquisa de leptospirose do CPqGM, realiza uma vigilância ativa no Hospital Couto Maia em Salvador-Ba, onde foi recrutado 4612 casos suspeitos para leptospirose. Destes 4612 foi confirmado o diagnóstico de 1853 (40%) utilizando pelo menos um dos três métodos de diagnóstico (MAT, Hemocultura, qPCR). Dentre os casos confirmados, 1759 (95%) foram confirmados pelo MAT. A sensibilidade do MAT foi diferente entre as amostras aguda e convalescente, sendo 60% na fase aguda e 97% na fase convalescente. O sorogrupo Icterohaemorrhagiae foi o mais prevalente (90%) dos casos confirmados para MAT. Durante o período do estudo foram coletadas 1133 hemoculturas e destas 203 (18%) foram positivas, sendo possível isolar leptospiras de 93/203 (45%) das hemoculturas, as quais foram soro-agrupadas com soros heterológos de coelho. A concordância entre o sorogrupo encontrado no MAT e na soro-agrupagem foi de 80%. Os achados mostram que existe uma concordância significante entre o sorogrupo encontrado pelos dois métodos, o que indica que o painel de cepas utilizado no MAT apresenta uma ótima cobertura para os sorogrupos prevalentes na região. A predominância de um sorogrupo facilitou quanto a tomadas de decisões para prevenção e controle, assim como facilita para o desenvolvimento de novos testes de diagnóstico e vacinas mais direcionados. / Leptospirosis is a re-emerging zoonotic disease of global importance, with 1,2 million cases reported each year. Diagnosis of leptospirosis is often difficult given the nonspecific disease presentation. In order to compare the performance of the two gold standard diagnostic tests for leptospirosis, the group enrolled 4612 patients with suspected leptospirosis during active surveillance at the state infectious disease reference hospital, Hospital Couto Maia, in Salvador, Bahia between 1996 and 2013. Of these, was confirmed Leptospira infection in 1853 (40%) using at least one of three diagnostic methods (microagglutination (MAT), blood culture, and qPCR). Was confirmed 1759 (95%) cases using only the MAT assay, and identified the serogroup Icterohaemorrhagiae as the infective agent in 90% of MAT positive samples. It was determined the sensitivity of the MAT was 60% for acute phase samples and increased to 97% for convalescent samples. Within this study period, it was possible to collect 1133 blood cultures and was isolated leptospires from 93 of 203 (45%) of blood cultures, and determined the serotype using heterologous rabbit sera. The concordance between the infective serogroup identified using hemoculture and MAT techniques was 80%. This result indicates that the panel of 11 strains used in the MAT represents a majority of the infective serogroups causing disease in our study population. The predominance of a single serogroup in symptomatic cases informs the development of new diagnostic tests and novel vaccines to prevent leptospirosis in Brazil.

Submitted by MARIA EDUARDA CHIARADIA FURQUIM null (mecfurquim@yahoo.com.br) on 2016-12-12T22:31:12Z No. of bitstreams: 1 Dissertação Maria Eduarda Chiaradia Furquim 2016.pdf: 2147185 bytes, checksum: 9cbd94cd959a4435ddb99f33983dbb76 (MD5) / Approved for entry into archive by Felipe Augusto Arakaki (arakaki@reitoria.unesp.br) on 2016-12-15T15:41:05Z (GMT) No. of bitstreams: 1 furquim_mec_me_jabo.pdf: 2147185 bytes, checksum: 9cbd94cd959a4435ddb99f33983dbb76 (MD5) / Made available in DSpace on 2016-12-15T15:41:05Z (GMT). No. of bitstreams: 1 furquim_mec_me_jabo.pdf: 2147185 bytes, checksum: 9cbd94cd959a4435ddb99f33983dbb76 (MD5) Previous issue date: 2016-11-18 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Com o intuito avaliar a frequência de aglutinas anti-Leptospira dentre as amostras analisadas no Laboratório de Brucelose e Leptospirose da Unesp, Câmpus Jaboticabal, realizou- se uma investigação retrospectiva dos resultados de exames sorológicos para diagnóstico de leptospirose entre os anos de 2007 e 2015. Neste período foram recebidas 24.483 amostras bovinas, 1.855 amostras equinas, 819 amostras caninas, 532 amostras ovinas, 230 amostras caprinas e 123 amostras suínas. As amostras bovinas provinham de 21 estados brasileiros e a frequência de reagentes foi de 35,30% (IC 95%: 34,70% - 35,90%), sendo o sorogrupo Sejroe o mais relevante. Das amostras caninas, a maioria foi originaria do Estado de São Paulo e a frequência de amostras reagentes foi de 23,93% (IC 95%: 21,01% - 26,85%), sendo o sorogrupo Canicola o mais relevante. Nos equinos, a frequência de amostras reagentes encontrada foi de 46,95% (IC 95%: 44,68% - 49,23%), sendo o sorogrupo Icterohaemorrhagiae o mais importante nesta espécie. A frequência de reagentes encontrada nas amostras das espécies ovina, caprina e suína foi 7,32% (IC 95%: 5,12% - 9,55%), 1,30% (IC 95%: 0,0044% - 3,764%) e 6,03% (IC 95%: 1,70% - 10,37%), respectivamente, e o sorogrupo de maior ocorrência nesses animais foi Icterohaemorrhagiae. Tais resultados permitem confirmar o caráter endêmico da leptospirose em território brasileiro, bem como evidenciar a importância dos hospedeiros de manutenção do agente na disseminação da doença. / In order to evaluate the frequency of anti-Leptospira agglutinins among the samples analyzed in the Leptospirosis and Brucellosis Laboratory, Unesp, Jaboticabal Campus, a retrospective investigation of the results of serological tests for leptospirosis between the years 2007 and 2015 was performed. During this period, 24,483 bovine samples, 1,855 equine samples, 819 canine samples, 532 sheep samples, 230 goats samples and 123 swine samples were received. Bovine samples came from 21 Brazilian states and the frequency of reactors was 35.30% (95% CI: 34.70% - 35.90%) and serogroup Sejroe was the most relevant. Of canine samples, most were originally from the state of São Paulo and the frequency of reacting samples was 23.93% (95% CI: 21.01% - 26.85%), the serogroup Canicola was the most relevant. In horses, the frequency of reagent samples was found to be 46.95% (95% CI: 44.68% - 49.23%), the serogroup Icterohaemorrhagiae was the most important in this species. The frequency of reactors among of sheep, goats and swine samples was 7.32% (95% CI: 5.12% - 9.55%), 1.30% (95% CI: 0.0044% - 3.764%) and 6.03% (95% CI: 1.70% - 10.37%), respectively, and the serogroup most frequent in these animals was Icterohaemorrhagiae. These results confirm the endemicity of leptospirosis in Brazil, as well as highlight the importance of the agent maintenance hosts in the dissemination of the disease.

Orientador: Luís Antonio Mathias / Resumo: Com o intuito avaliar a frequência de aglutinas anti-Leptospira dentre as amostras analisadas no Laboratório de Brucelose e Leptospirose da Unesp, Câmpus Jaboticabal, realizou- se uma investigação retrospectiva dos resultados de exames sorológicos para diagnóstico de leptospirose entre os anos de 2007 e 2015. Neste período foram recebidas 24.483 amostras bovinas, 1.855 amostras equinas, 819 amostras caninas, 532 amostras ovinas, 230 amostras caprinas e 123 amostras suínas. As amostras bovinas provinham de 21 estados brasileiros e a frequência de reagentes foi de 35,30% (IC 95%: 34,70% - 35,90%), sendo o sorogrupo Sejroe o mais relevante. Das amostras caninas, a maioria foi originaria do Estado de São Paulo e a frequência de amostras reagentes foi de 23,93% (IC 95%: 21,01% - 26,85%), sendo o sorogrupo Canicola o mais relevante. Nos equinos, a frequência de amostras reagentes encontrada foi de 46,95% (IC 95%: 44,68% - 49,23%), sendo o sorogrupo Icterohaemorrhagiae o mais importante nesta espécie. A frequência de reagentes encontrada nas amostras das espécies ovina, caprina e suína foi 7,32% (IC 95%: 5,12% - 9,55%), 1,30% (IC 95%: 0,0044% - 3,764%) e 6,03% (IC 95%: 1,70% - 10,37%), respectivamente, e o sorogrupo de maior ocorrência nesses animais foi Icterohaemorrhagiae. Tais resultados permitem confirmar o caráter endêmico da leptospirose em território brasileiro, bem como evidenciar a importância dos hospedeiros de manutenção do agente na disseminação da doença. / Mestre

Made available in DSpace on 2014-12-17T15:01:23Z (GMT). No. of bitstreams: 1 SuzianiCMD_DISSERT.pdf: 1628830 bytes, checksum: 0dae9562578f76707994b6859cfb7472 (MD5) Previous issue date: 2010-10-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / This study aimed to investigate the foam mat drying process of pineapple and mango pulp, as well as to evaluate the final product quality. Initially, the selection of fruit and additives was conducted based on density and stability determinations of mango, seriguela, umbu and pineapple foams. After selecting pineapple and mango for further studies, the fruit pulps and fruit foams were characterized in regard to their physicochemical composition. The temperature (60oC or 70oC) and the foam thickness (4 and 11 mm) were evaluated in accordance to the obtained drying curves and after model adjustment. Mango and pineapple powders obtained at the best process conditions were characterized in regard to their physicochemical composition, solubility, reconstitution time. Yoghurts were prepared with the addition of pineapple and mango powders and they were evaluated for their sensory acceptance. Results show that the best drying rates were achieved by using 70o C and layers 4mm thick for both fruits. The Page model successfully fitted the drying experimental data and it can be used as a predictive model. Pineapple and mango powders showed acid pH, high soluble solids content, low water activity (approx. 0.25), lipids between 1.46% and 2.03%, protein around 2.00%, and ascorbic acid content of 17,73 mg/100g and 14.32 mg/100g, for mango and pineapple, respectively. It was observed higher ascorbic acid retention for pineapple and mango powders processed at 70o C, which would be explained by the lower drying time applied. The fruit powders exhibited high solubility and fast reconstitution in water. The sensory acceptance indexes for yoghurts with the addition of both fruit powders were higher than 70%, which reflect the satisfactory product acceptance / O presente trabalho teve como objetivo estudar o processo de secagem das polpas de abacaxi e manga atrav?s do processo foam-mat, avaliando-se a qualidade do produto final obtido. Inicialmente foi realizado a sele??o das frutas e dos aditivos a serem empregados a partir da determina??o da densidade e estabilidade das espumas preparadas com polpas de manga, seriguela, umbu, e abacaxi . Definidas as frutas, manga e abacaxi os aditivos, Liga Neutra Artesanal e Emulsificante e a formula??o, tanto as polpas como as espumas foram caracterizadas quanto a composi??o f?sico-qu?mica. Estudou-se o efeito da temperatura (60 e 70?C) e espessura da camada de espuma (4 e 11 mm) sobre a secagem , avaliando-se o comportamento das curvas de secagem e ajustando-se modelos da literatura ao dados experimentais. Para os p?s de manga e abacaxi obtidos nas melhores condi??es de processo foi realizada a caracteriza??o f?sico qu?mica, a determina??o da solubilidade e tempo de reconstitui??o e a an?lise sensorial de iogurtes saborizados com os mesmos. Conforme os resultados as condi??es que favoreceram as melhores taxas de secagem foi a temperatura de 70?C e espessura da camada de espuma de 4 mm para ambas as frutas. O modelo de Page se ajustou de forma bastante satisfat?ria aos dados experimentais das curvas de secagem podendo ser utilizado para fins preditivos. Os p?s de manga e abacaxi processados na temperatura de 70?C apresentaram pH ?cido, s?lidos sol?veis elevados, baixa atividade de ?gua (0,25 em m?dia), teor de lip?dios entre 1,46% e 2,03% e prote?na em torno de 2%,.al?m de teor de vitamina C de 17,73 mg/100g para manga e 14,32 mg/100g para abacaxi. Avaliando-se o impacto da temperatura sobre a a vitamina C, observou-se que os p?s de abacaxi e manga desidratados a 70? C apresentaram maior reten??o de ?cido asc?rbico devido ao menor tempo de exposi??o das espumas ao calor. Os produtos em p? apresentaram elevada solubilidade e r?pida reconstitui??o em ?gua. A avalia??o sensorial dos iogurtes saborizados apresentaram ?ndices de aceita??o de todos os quesitos avaliados superiores a 70% demonstrando a boa aceita??o do produto

Denna rapport skrevs som en del av kursen TDDD96 Kandidatprojekt i Programvaruutveckling. Den dokumenterar arbetet med projektet Matbit: en applikation vars syfte var att samla webbutikerna från de största matvarukedjorna i Sverige i en och samma app för enklare jämförelse av varor. Projektgruppens arbete var specifikt inriktat på att dölja de webbsidor som användaren slussades till när hen valde att beställa hem sin varukorg och i stället visa upp ett eget gränssnitt i appen, medan kommunikationen med de faktiska webbsidorna skedde i bakgrunden. Resultatet av arbetet blev en första version av ett gränssnitt med nästintill helt fungerande kommunikation med Coop. Rapporten inleds med en introduktion till projektet och dess syfte, följt av bakgrund hos både kunden och projektgruppen. Sedan följer teoretisk bakgrund och en genomgång av de metoder som låg till grund för projektarbetet. Vidare presenteras resultatet av projektarbetet med en diskussion och slutsatser. Slutligen innehåller rapporten individuella delar skrivna av projektdeltagarna om ett valfritt ämne kopplat till projektet eller dess arbetsmetoder.