The molecular mechanism of complementary sex determination in the haplodiploid organisms is poorly understood and has only been described in the honeybee Apis mellifera. In the haplodiploid system, males develop from unfertilized eggs and females from fertilized eggs. However, in some rare cases, diploid males develop from fertilized eggs. They can be distinguished from diploid and haploid males at the molecular level since they are heterozygous like females but are homozygous, like haploid males, at the sex determination locus. In this project, Osmia bicornis was chosen as the model organism, and the aim is to identify the complementary sex determination locus which should be homozygous in all diploid males. Bee nests were collected from the bees' natural habitat, and potential diploid males were identified. Data analysis of whole-genome sequencing on 17 potential diploid males was performed, which identified 80 candidate sex determination loci with 259 genes. Homologs of the Csd gene in A. mellifera were identified but not found in any candidate complementary sex determination loci.

The problem with logging migraines is that patients often forget, or simply choose not to log their migraines. To solve this problem, we investigate in this thesis whether gamification can make a difference in their motivation. To investigate whether gamification makes a difference, an AB test was performed. An A prototype, without gamification, and a B prototype, without gamification, were developed in the initial phases of this thesis, these were tested on people outside of the target group. Motivation was measured using Self Determination Theory and its sub-theory Organic integration. Gamification elements chosen to use in the B-prototype is based on the desired feel of the prototype, the elements included an avatar, badges, and progress bars. The A/B-test was performed on 10 people, 5 for each prototype, and all the participants were diagnosed with migraine and had some previous experience of logging their migraines and symptoms. The result includes interview questions about their experience, observations of user tests and answers from a questionnaire. The results showed no significant overall difference between the two prototypes; however, discussion and some hypotheses can be created by the results. Most difference between the results can be seen in integration regulation, a subcategory of motivation, where the prototype without gamification got a higher result. / Detta examensarbete kommer att undersöka möjligheten att använda gamification i mobila applikationer för migränpatienter, för att se om det kommer att hjälpa migränpatienter med deras loggning. Problemet med att logga migrän är att patienter ofta glömmer, eller helt enkelt väljer att inte logga sin migrän. För att lösa detta problem undersöker vi i detta examensarbete om gamification kan göra skillnad i deras motivation. För att undersöka om gamification gör skillnad gjordes ett AB-test. En A-prototyp, utan gamification, och en B-prototyp, utan gamification, utvecklades i de inledande faserna av detta examensarbete, dessa testades på personer utanför målgruppen. Motivation mättes med hjälp av Self Determination Theory och dess underteori Organic integration. Gamification-element som valts att använda i B-prototypen är baserade på den önskade känslan av prototypen, elementen inkluderade en avatar, badges och progressbar. A/B-testet utfördes på 10 personer, 5 för varje prototyp, alla deltagare var diagnostiserade med migrän sedan innan och hade tidigare erfarenhet av att logga sin migrän och symptom. Resultatet inkluderar intervjufrågor om deras upplevelse, observationer av användartester och svar från en frågeenkät. Resultaten visade ingen signifikant skillnad mellan de två prototyperna, men diskussion och vissa hypoteser kan skapas av resultaten. Mest skillnad mellan resultaten kan ses i integration regulation, en underkategori av motivation, där prototypen utan gamification resultat visa högre nummer på likert-skalan.

Studien undersöker hur den stress som idrottare upplever kopplat till idrottsutövandet påverkas av olika motivationsfaktorer, samt hur det återspeglas i deras upplevda prestation. Urvalet bestod av pojkar och flickor i åldern 16 - 20 år som går på idrottsgymnasium. Enkäten besvarades av totalt 87 personer (49 kvinnor och 38 män) som var aktiva inom längdskidor (n = 32), friidrott (n = 34) samt orientering (n = 21). Studiens frågeställningar besvarades med hjälp av självskattningsformulären Sports Motivation Scale II (SMS-II), Attainment of Sport Achievement Goals Scale (ASAG) samt Perceived Stress Reactivity Scale for Adolescent Athletes (PSRS-AA). En korrrelationsanalys visade att det fanns ett svagt samband mellan grad av självbestämmande motivation (RAI) och upplevd prestation samt ett svagt negativt samband mellan stress och självbestämmande motivation. Mer sofistikerade analyser kunde inte bekräfta någon samvariation mellan dessa variabler. Däremot kan man urskilja hur könsskillnader påverkar graden av upplevd stress. Där det var kvinnor som skattade sig högre. Ytterligare studier med större populationer behövs för att prova dessa hypoteser. / The study examines how the stress that athletes experience connected to the exercise of sports is affected by various motivational factors, as well as how it is reflected in their perceived performance. The selection consisted of boys and girls aged 16 - 20 who attend a sports high school. The questionnaire was answered by a total of 87 people (49 women and 38 men) who were active in cross-country skiing (n = 32), athletics (n = 34) and orienteering (n = 21). The study's questions were answered using the self-assessment forms Sports Motivation Scale II (SMS-II), Attainment of Sport Achievement Goals Scale (ASAG) and Perceived Stress Reactivity Scale for Adolescent Athletes (PSRS-AA). A correlation analysis showed that there was a weak relationship between degree of self-determination motivation (RAI) and perceived performance and a weak negative relationship between stress and self-determination motivation. More sophisticated analyzes could not confirm any covariation between these variables. However, it is possible to discern how gender differences affect the degree of perceived stress. Where it was women who rated themselves higher. Further studies with larger populations are needed to test these hypotheses.

Thesis: M.S., Massachusetts Institute of Technology, Department of Chemical Engineering, 1975 / Includes bibliographical references. / M.S. / M.S. Massachusetts Institute of Technology, Department of Chemical Engineering

Self-determination is a peoples' right to freely determine their political, economic and cultural destiny without external interference. However, the cultivation of a culture of respect for self-determination remains the greatest challenge to post-colonial Africa. Dictatorships and other oppressive regimes very substantially affected Africa's efforts to develop a culture of constitutionalism and respect for the right of peoples to selfdetermination. Most African countries typify the failed effort of trying to establish an enduring democracy and respect for the right of peoples to take part in the government. After five decades of transition from colonialism to constitutional democracy, most African peoples are still under the yoke of governments they consider undesirable or oppressive. This work primarily sets out to investigate if the denial of the right of peoples to self-determination justifies the use of force to secure such a right. Since independence, Africa has experienced armed rebel groups seeking either to effect radical transformation of the whole state or to separate from the state to which they belong in order to create a new state. In the main, this study explores the extent to which rebel groups acting on behalf of peoples are or are not allowed to use force for the attainment of self-determination. The thesis begins with an historical development of the right to self-determination in international law. It initially examines how self-determination has developed from a political principle to a legal right. Despite the fact that self-determination is one of the core principles of the UN Charter, there are still many controversies over its precise meaning, scope and application. The thesis considers the two aspects of selfdetermination: external self-determination and internal self-determination. The external aspect implies the right of people to form a new, sovereign and independent state, whereas the internal aspect implies the right of people to participate in the political framework of an existing state. The thesis also assesses the state of the academic literature over the right of peoples to self-determination, with a view to determining whether the right can be used by a group of people whose internal self-determination has been denied to effect secession from the state. It advocates that, outside the colonial context, the right of self-determination does not equal to a "right to secession and independence". The thesis argues, however, that in exceptional circumstances such as gross violations of human rights and the denial of internal self-determination, people should be endowed with a right to secession in the manifestation of a right to unilateral secession as a remedy of such injustices. The thesis further turns to the mechanisms for the protection of the peoples' right to self-determination, the problems and challenges in Africa. The challenges do not only include the legality of the use of force by rebel groups and national liberation movements in seeking to attain self-determination, but also the right of other states to assist them in their struggles. The work probes the nature of international law and critically assesses whether the persistent denial of demands for self-determination led to calls for drastic remedies, including the use of armed force. Before this theory is critically assessed, the thesis defines the differences between national liberation movements and rebel groups. It argues that as far as self-determination struggles are concerned, there must be representative organisations acting on behalf of people whose right of self-determination has been denied. In the light of these contentions, the study examines the general ban on the use of force as laid down by the UN Charter, and finds that the Charter does not expressly refer to self-determination as a situation where people may resort to the use of force for the attainment of such a right. It then turns to the history of and circumstance surrounding the use of force, examines the jus ad bellum regarding "liberation struggles", and concludes that the use of force by national liberation movements against colonial and racist regimes has strong theoretical foundations and support in state practice. Outside of the colonial and apartheid contexts, however, the argument that rebels acting on behalf of oppressed peoples may legitimately use force in pursuit of selfdetermination thus remains ambiguous. In that context, this thesis examines the practice relating to the use of force by rebel groups and the laws of war provisions that apply in civil wars, and concludes that none of them proves that the international community of states accepts rebels' right to use force as a legal entitlement. Finally, based on the lessons learned from and lacunae identified in all norms relating to the enforcement mechanisms of the right of self-determination, this study concludes with a set of suggestions and recommendations. / LLD (Law), North-West University, Potchefstroom Campus, 2015

Self-determination is a peoples' right to freely determine their political, economic and cultural destiny without external interference. However, the cultivation of a culture of respect for self-determination remains the greatest challenge to post-colonial Africa. Dictatorships and other oppressive regimes very substantially affected Africa's efforts to develop a culture of constitutionalism and respect for the right of peoples to selfdetermination. Most African countries typify the failed effort of trying to establish an enduring democracy and respect for the right of peoples to take part in the government. After five decades of transition from colonialism to constitutional democracy, most African peoples are still under the yoke of governments they consider undesirable or oppressive. This work primarily sets out to investigate if the denial of the right of peoples to self-determination justifies the use of force to secure such a right. Since independence, Africa has experienced armed rebel groups seeking either to effect radical transformation of the whole state or to separate from the state to which they belong in order to create a new state. In the main, this study explores the extent to which rebel groups acting on behalf of peoples are or are not allowed to use force for the attainment of self-determination. The thesis begins with an historical development of the right to self-determination in international law. It initially examines how self-determination has developed from a political principle to a legal right. Despite the fact that self-determination is one of the core principles of the UN Charter, there are still many controversies over its precise meaning, scope and application. The thesis considers the two aspects of selfdetermination: external self-determination and internal self-determination. The external aspect implies the right of people to form a new, sovereign and independent state, whereas the internal aspect implies the right of people to participate in the political framework of an existing state. The thesis also assesses the state of the academic literature over the right of peoples to self-determination, with a view to determining whether the right can be used by a group of people whose internal self-determination has been denied to effect secession from the state. It advocates that, outside the colonial context, the right of self-determination does not equal to a "right to secession and independence". The thesis argues, however, that in exceptional circumstances such as gross violations of human rights and the denial of internal self-determination, people should be endowed with a right to secession in the manifestation of a right to unilateral secession as a remedy of such injustices. The thesis further turns to the mechanisms for the protection of the peoples' right to self-determination, the problems and challenges in Africa. The challenges do not only include the legality of the use of force by rebel groups and national liberation movements in seeking to attain self-determination, but also the right of other states to assist them in their struggles. The work probes the nature of international law and critically assesses whether the persistent denial of demands for self-determination led to calls for drastic remedies, including the use of armed force. Before this theory is critically assessed, the thesis defines the differences between national liberation movements and rebel groups. It argues that as far as self-determination struggles are concerned, there must be representative organisations acting on behalf of people whose right of self-determination has been denied. In the light of these contentions, the study examines the general ban on the use of force as laid down by the UN Charter, and finds that the Charter does not expressly refer to self-determination as a situation where people may resort to the use of force for the attainment of such a right. It then turns to the history of and circumstance surrounding the use of force, examines the jus ad bellum regarding "liberation struggles", and concludes that the use of force by national liberation movements against colonial and racist regimes has strong theoretical foundations and support in state practice. Outside of the colonial and apartheid contexts, however, the argument that rebels acting on behalf of oppressed peoples may legitimately use force in pursuit of selfdetermination thus remains ambiguous. In that context, this thesis examines the practice relating to the use of force by rebel groups and the laws of war provisions that apply in civil wars, and concludes that none of them proves that the international community of states accepts rebels' right to use force as a legal entitlement. Finally, based on the lessons learned from and lacunae identified in all norms relating to the enforcement mechanisms of the right of self-determination, this study concludes with a set of suggestions and recommendations. / LLD (Law), North-West University, Potchefstroom Campus, 2015

Determination of Parenthood (Abstract) The determination of parenthood is undoubtedly a very topical issue. There are significant changes which are consequences of modern society approach to the family life as well as of massive progress in the field of medical science and genetics. The development in these areas has brought new concepts such as baby-boxes, assisted reproduction and surrogacy. Now it is up to the legislation to provide rules within the legal framework for these new institutions to steady parental relationships and to secure their protection as well. The aim of this thesis is to familiarize the reader with Czech legal regulation of determination of parenthood by means of its description and analysis. Another aim of the work is to compare this legal regulation with regulation in other countries, in particular in Germany and to find similarities and differences between them. Finally the thesis highlights new institutions within determination of parenthood. This thesis consists of nine chapters which deal with particular parts of its subject-matter. The opening chapter discusses the relationship between a parent and a child both theoretically and historically. Apart from that it also describes legal regulations applicable in the Czech Republic and Germany in the course of time. The second...

Determination of Parenthood (Abstract) The topic determination of parenthood today is very actual nowadays and it is not possible to neglect its importance. Changes taking place both in the social life of modern times, and in the field of medicine is necessary to constantly reflect and to adapt them to the legal order. Concepts such as assisted reproduction, surrogacy, concealed birth and baby-boxes are a common part of human society and it is not possible to avoid dealing with the situation around them. The aim of this thesis is to summarize the Czech legislation of determination of parenthood, to mention problematic situations that may arise in connection with it and describe possible solutions to these situations. The another aim then is to acquaint the reader with the newly developing institutions, such as assisted reproduction, surrogacy, concealed birth, baby boxes, etc., and analysis of their current legal status supplemented by comparison of legislation in other European and non-European countries, which could serve as inspiration for future development in the Czech legal order. The first chapter deals with family law, family and relationships between parents and children both from a theoretical and historical point of view. The second chapter is devoted to an overview of the most important changes...

- Determination of Parenthood The topic of my thesis is Determination of Parenthood. With this topic are connected problems not only legal, but often also social and ethical. Parenthood affects all of us and it's an important aspect of human life. That's why there should be legal assurance in relationship between parent and child. Development in the field of medicine is still ahead (mainly thanks to methods of assisted reproduction and DNA analysis) and the rules of law should respond and deal with it. The aim of this thesis is describe and become acquainted with problematics of determination of parenthood, valorize how legislation responds to modern development and to propose possible changes. Thesis is prepared on the basis of existing legislation on the date of submission of the thesis. Thesis is divided into seven main chapters, which are divided to further subsections. First chapter deals with concept of parenthood and methods of determination. Second chapter describes historical development of legislation on Czech territory, since Roman law to recodification of civil law. Related third chapter concisely summarizes national and international legal sources of current legislation. Chapter four is about determination of maternity and problems of surrogacy, anonymous childbirth, concealed birth...

Sex determination among reptiles is a very variable matter across it's taxa. We meet there temperature sex determination and genotypic sex determination with many independent transitions between them. It is a group suitable to study evolution of sex determination, sex chromosomes and sex determination genes. Rare cases of sex reversal caused by extreme incubation temperature or exogenous hormones have been reported in recent years. In case of Acrodont lizard, Pogona vitticeps, was reported sex reversal caused by high incubation temperatures. Our purpose was to repeat the experiment, mainly due to insufficient conclusiveness of used methods. We wanted to expand the experiment by hormonal reversal, studying persistence of sex reversal to maturity and fertility of reversed individuals. We managed successfully to demonstrate sex reversal in both treatments by histological examination. Individuals with discordant phenotypic and genotypic sex were breed till one year of life, which demonstrate persistence of reversal. Our outcomes are concordant with most recent work on this species and show full functional phenomenon of sex reversal with reptiles, which studying could contribute to our understanding of evolution of sex determination.