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近年來因網際網路盛行，各行各業皆面臨了全球國際化的競爭，於是虛擬平台的經營便成為大家努力的方向，如何提高虛擬平台的網路流量已成為平台經營者所重視的議題。 拜網際網路應用的成熟，不動產業雖然很傳統，但也不得不開始運用網際網路提供服務，並且這些服務越來越多元化，提供物件資訊、透過討論區討論物件內容、讓經紀人以社群方式存在，在平台上提共買賣不動產的相關知識，消費者可透過平台買賣物件，尋找物件。故研究如何增加虛擬平台的流量，將是未來平台經營者在提升平台品質上有效的方法。本研究將提出影響網站流量策略的研究架構，根據Alexa網站提供覆蓋數（Reach）與頁面瀏覽數（Pageview），以五個不動產個案公司的網站流量進行分析。 本研究流量分析的架構如下：首先就文獻資料了解影響網站流量的因素，對照個案公司在這些影響網站流量的因素上的表現，進而了解何種因素是確實影響網站流量。 經分析結果顯示，目前為了使網站流量表現良好，各家個案公司，都會使用搜尋引擎、關鍵字、SEO操作等工具，所以在工具上各家個案公司表現差異不大，惟廣告行銷的投入，對整體網站流量實際上才是重要的影響，另外，許多專家或者是文獻，認為免費的商業模式是網際網路平台經營時重要的方式，但研究發現卻是平台業者收取一點費用是客戶可以接受的，平台的內容與服務，實際會提升虛擬平台的網站流量；經分析顯示，外部網站結盟有助於提升虛擬平台網站流量，希望藉由本研究的分析結果提供給不動產虛擬平台經營者在網站流量策略上的參考建議。 / In the internet era, the most significant issue for platform operators is how to design and manage the virtual platform to increase the web traffic flow. The diversity of online services that offer information and discussion through Internet forum allows real estate agents to provide product knowledge through different kinds of virtual community. Consumers can search, purchase and sell real estate items through these virtual platforms. Consequently, virtual service providers endeavor to enhance platform operation and service quality to attract visitors. This research proposes a research framework to investigate determinants of web traffic. Based on literature review and related researches this study analyzed the causes and effects of web traffic. A few traffic-affecting factors were identified. These factors include: content management, marketing strategies, promotion strategies, and business models. Next, five different types of web sites in the real estates industry in Taiwan were selected for multiple case studies. The cross case analysis revealed that all companies have applied search engine, keywords, and SEO (xxx) to enhance users’ access to the web sites. The impact of technology use and system functionality on users’ intention to participate is of no great concern. However, the advertising effort seems to be the key determinant for boosting website traffic. Further, although several researches consider free-of-charge as a winning business model the analysis results reveal that well-managed contents can bring in customers who are willing to pay for the services. The results also demonstrate that external alliance of websites can assist the growth of the traffic flow on a virtual platform. Since Internet is widely used these decades, various industries have been occurred the global competitions. The most significant issues for platform operators are how to conduct virtual platform well and how to increase web flow rate of virtual platform. The mature usage of Internet initiates the real estate industry to offer services through Internet. The diversity of online services that offer information and discussion through Internet forum allow real estate agents to provide real estate related knowledge through community websites. Consumers can also search, purchase and sell real estate items through the virtual platform. Consequently, the research of how to increase virtual platform flow will enhance platform operators efficiently advance service quality of platforms. This research proposes a research framework to investigate what determinants effect web traffic strategy and analyze web traffic of five real estate companies by collecting Reach and Pageview from Alexa website. The Framework of this research is as follows. First, we analyze the causes and effects of web traffic based on literature. Next, we compare the effects of web traffic among selected cases to recognize which determinants influence web traffic. The analytical results reveal that all companies use search engine, keywords, and SEO to enhance the performance of web traffic. The difference of all tools among cases is insignificant. However, the advertising effort is the key determinant for whole website traffic. In addition, many researchers and literature consider free is the major business model for platform operators. Nevertheless, this research discovers the consumers accept for charging few fees and the content and service of the platform increase the traffic. The results also demonstrate that external alliance of websites assists the increment of traffic for virtual platform. Finally, this study aims to provide strategic suggestions to companies in virtual estimate industry.

Šiame magistriniame darbe yra pateikiama CSS (Cascading Style Sheeets) apžvalga ir mokymosi galimybių analizė. Taip pat darbe yra pateikiami tyrimo, skirto išsiaiškinti Lietuvoje gyvenančių suinteresuotų asmenų poreikius CSS mokymo kurso teikimui internete ir kurso teikimo aplinkai. Atsižvelgiant į tyrimo rezultatus parengtas pedagoginio kurso teikimo scenarijus ir kursui teikti skirtos virtualios aplinkos projektas bei dirbančios aplinkos vartotojo gidas. / Main aim of this work is to create CSS web-based course pedagogical model and develop web based learning environment ideal for this course. In this document’s project part you will find course, named “CSS usage for web pages creation process”, pedagogical model and web-based learning environment creating process detailed projecting steps. In the end of document you will find created learning environment using and administrating manual.

This thesis examines the production of the Middle English poetic manuscript. It analyses the mise-en-page of manuscripts created during a crucial period for book production, immediately after 1400, when there was a sudden explosion in the production of vernacular manuscripts of literary texts, when the demand for books increased, and the commercial book trade swiftly followed. It offers a close analysis of the mise-en-page of the manuscripts of three central authors: Chaucer’s, Gower’s, and Hoccleve’s manuscripts were at the heart of this sudden flourishing and were, crucially, produced when scribal methods for creating the literary page were still unformed. Previous studies have focused on the localised readings produced by single scribes, manuscripts, or authors, offering a limited examination of broader trends. This study offers a wider comparison: where individual studies offer localised analysis, the multi-textuality of this thesis offers broader perceptions of book production and of scribal responses to the new literary texts being produced. In analysing the layout of seventy-six manuscripts, including borders, initials, paraphs, rubrics, running titles, speaker markers, glosses and notes, this thesis argues that scribes were deeply concerned with creating a manuscript page specifically to showcase texts of poetry. The introduction outlines current scholarship on mise-en-page and defines the scribe as one who offers an individual response to the text on the page within the context of the inherited, commercial, and practical practices of layout. The three analytical chapters address the placement of the features of mise-en-page in each of the seventy-six manuscripts, each chapter offering three contrasting manuscript situations. Chapter 1 analyses the manuscripts of Chaucer, who left no plan for the look of his page, causing scribes to make decisions on layout that illuminate fifteenth-century scribal responses to literature. These are then compared to the manuscripts of Gower in Chapter 2, directly or indirectly supervised by the poet, which display rigorous uniformity in their layout. This chapter argues that scribes responded in much the same way, despite the strict control over meaning. Chapter 3 focuses on Hoccleve’s autograph manuscripts which are unique in demonstrating authorial control over layout. This chapter compares the autograph to the non-autograph manuscripts to argue that scribal responses differed from authorial intentions. Each of the three chapters analyses the development of mise-en-page specifically for literary texts. Focussing on the mise-en-page, this thesis is able to compare across a range of texts, manuscripts, scribes, and authors to mount a substantial challenge to current perceptions that poetic manuscripts were laid out in order to assist readers’ understanding of the meaning of the texts they contain. Instead, it argues that though there was a concern with representing the nuances of poetic meaning, often scribal responses to poetry were bound up with presenting poetic form.

PsbO (manganese-stabilizing protein) is the largest extrinsic protein of photosystem II, located on the lumen side of photosystem. It is present in all known oxyphototrophic organisms. PsbO facilitates photosynthetic water splitting, which takes place in an oxygen evolving center (Mn4CaO5 cluster) of photosystem II. This work is focused on PsbO of higher plants and its isoforms, particularly their evolution and functions. Bioinformatic analyses revealed that majority of higher plants express exactly two psbO isoforms. A phylogenetic tree of PsbO sequences has an unusual topology. The two paralogous isoforms do not diverge at the base of the phylogenetic tree, as anticipated, but rather at the end of particular branches, at the level of family or lower taxonomic unit. In this work we propose and discuss several hypotheses concerning evolution of PsbO isoforms. The work further includes detailed analysis and identification of protein spots assigned to PsbO on 2D IEF-SDS PAGE gels of potato thylakoid proteins. We identified predominant version of PsbO isoform in most of the spots. We did not succeed to find any posttranslational modification. We optimized a method of psbO expression in E. coli and subsequent purification, which yielded relatively big amount of properly folded recombinant protein. Analysis of...

This master's thesis deals with the cultural-political weekly magazine Literární noviny. The first part is dedicated to the forms of the Literární noviny from 1927, when the magazine emerged, to 1969, when it ceased to be published for twenty-one years. The main focus of the thesis is its second part, which explores the life of the Literární noviny in the 90s. On 5 April, 1990 the weekly was renewed as an appendix of the Lidové noviny, and two years later it became an independent magazine. Its chief editor was Vladimír Karfík until 1999. I did an interview with Vladimír Karfík, which is attachment of this thesis. I concentrated on the weekly's editorial composition, especially on the sections and its thematic structure, but also on the social and cultural context of the 90s. The main research method used for analysing the composition of the thematic structure is quantitative content analysis. The research questions are related both to the topics that formed the content of the Literární noviny in the 90s, and their transformation within that decade. I also used the elements of qualitative analysis, to compare the transformation of the front page of the weekly magazine in the above mentioned period. The third section is dedicated to the feuilletons of Ludvík Vaculík. I focused on author's feuilletons...

Este trabalho é uma análise do hipertexto veiculado pela Internet sob a ótica da Análise do Discurso e da Semântica Histórica da Enunciação. Temos como ponto de partida uma discussão sobre os aspectos do suporte digital de textos e as mudanças que podem causar no processo de construção do significado. O objetivo é estudar os movimentos efetuados durante a navegação pela Internet e verificar se o processo de abertura e fechamento de páginas pode afetar a construção de sentidos. O corpus foi coletado de um Website jornalístico (CNN.com), o qual cobriu as primeiras horas posteriores ao ataque de 11 de Setembro nos EUA. Esse ato inédito contra o território americano inspirou um grande volume de produção de textos, além de a Internet ter sua primeira chance de cobrir um evento dessa proporção. Assim, tanto o ataque quanto a cobertura dessa mídia podem ter gerado elementos novos que contribuíram para a formação de outros métodos de se escrever e ler a notícia. Em nossa análise, observamos elementos verbais e não-verbais, bem como a importância desses componentes que não apenas dão forma à identidade do hipertexto, mas também contribuem para o processo de apreensão de sentidos. A ativação desses elementos proporciona à Internet um sistema de operação intertextual que pode demonstrar algumas mudanças na maneira em que as informações são processadas por aqueles que as produzem e pelos outros que as lêem. / This is an analysis of the hypertext in the Internet using principles of Discourse Analysis and Historic Semantics which starts from a discussion about some features of the digital text support and the changes it may be causing in the process of signification. The objective is to study the movements made during the navigation of the Internet and verify if the process of opening and closing pages may affect the meaning formation. The corpus has been collected from a journalistic Website (CNN.com) that covered the first hours after the attack of September 11th in the USA. This unprecedented act against the American territory inspired an enormous amount of text production and the Internet had the first chance of covering an event of such a magnitude. Therefore both the attack and this media coverage may have provided firsthand elements that may have helped to found different methods of writing and reading the news. In the analysis we have observed verbal and non-verbal elements and the importance of these components that not only format the identity of the hypertext but also contribute to the process of meaning apprehension. The activation of these elements gives the Internet a system to operate inter-textual relations that may show some changes in the way information is processed by ones who produce it and the ones who read it.

Made available in DSpace on 2016-04-28T20:15:32Z (GMT). No. of bitstreams: 1 2009 - Gisele Santos de Meireles.pdf: 1867896 bytes, checksum: 50898c1a65b34d41d9d4352322ce8d1e (MD5) Previous issue date: 2009-02-27 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / This work aimed, to determine the phenotypic analysis from sporulated oocysts of Cystoisospora felis by using a sequential method of cleaning techniques. By using SDS-PAGE at 10 and 12 % were separated 22 protein groups as: 26.65; 29.71; 31.79; 36.41; 44.03; 50.09; 56.08; 62.64; 73.65; 77.55; 85.34; 97.62; 98.66; 101.24; 104.21; 109.23; 110.56; 113.21; 138.48; 180.50; 206.81 and 244.51KDa belonged to estrutucture from C. felis sporulated oocysts. Western Blotting technique was performed after SDS-PAGE and were identified these antigenic proteins: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 and p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa from hyperimmune and natural infected rabbits by C. Felis respectively. / Este trabalho teve por objetivo, tra?ar um perfil prot?ico de oocistos esporulados de Cystoisospora felis, recuperados a partir do uso seq?encial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 10 e 12 % resultou na identifica??o de 22 grupos prot?icos de 26; 29; 31; 36; 44; 50; 56; 62; 73; 77,55; 85,34; 97,62; 98,66; 101,24; 104,21; 109,23; 110,56; 113,21; 138,48; 180,50; 206,81 e 244,51 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de coelho heter?logo anti-C. felis foi poss?vel desenvolver uma t?cnica de diagn?stico imunoenzimatico com western Blotting para identifica??o de animais infectados de maneira natural ou experimental com C. felis, identificando os seguintes prote?nas antig?nicas: p39.39; p42.18; p44.40; p47.82; p55.46; p58.75; p66.08; p77.41; p92.85; p99.58; p104.10; p112.84; p203.15 e p37.25; p38.80; p67; p69; p77; p93; p99; p103; p111.19; p202.66 KDa, respectivamente.

Este trabalho é uma análise do hipertexto veiculado pela Internet sob a ótica da Análise do Discurso e da Semântica Histórica da Enunciação. Temos como ponto de partida uma discussão sobre os aspectos do suporte digital de textos e as mudanças que podem causar no processo de construção do significado. O objetivo é estudar os movimentos efetuados durante a navegação pela Internet e verificar se o processo de abertura e fechamento de páginas pode afetar a construção de sentidos. O corpus foi coletado de um Website jornalístico (CNN.com), o qual cobriu as primeiras horas posteriores ao ataque de 11 de Setembro nos EUA. Esse ato inédito contra o território americano inspirou um grande volume de produção de textos, além de a Internet ter sua primeira chance de cobrir um evento dessa proporção. Assim, tanto o ataque quanto a cobertura dessa mídia podem ter gerado elementos novos que contribuíram para a formação de outros métodos de se escrever e ler a notícia. Em nossa análise, observamos elementos verbais e não-verbais, bem como a importância desses componentes que não apenas dão forma à identidade do hipertexto, mas também contribuem para o processo de apreensão de sentidos. A ativação desses elementos proporciona à Internet um sistema de operação intertextual que pode demonstrar algumas mudanças na maneira em que as informações são processadas por aqueles que as produzem e pelos outros que as lêem. / This is an analysis of the hypertext in the Internet using principles of Discourse Analysis and Historic Semantics which starts from a discussion about some features of the digital text support and the changes it may be causing in the process of signification. The objective is to study the movements made during the navigation of the Internet and verify if the process of opening and closing pages may affect the meaning formation. The corpus has been collected from a journalistic Website (CNN.com) that covered the first hours after the attack of September 11th in the USA. This unprecedented act against the American territory inspired an enormous amount of text production and the Internet had the first chance of covering an event of such a magnitude. Therefore both the attack and this media coverage may have provided firsthand elements that may have helped to found different methods of writing and reading the news. In the analysis we have observed verbal and non-verbal elements and the importance of these components that not only format the identity of the hypertext but also contribute to the process of meaning apprehension. The activation of these elements gives the Internet a system to operate inter-textual relations that may show some changes in the way information is processed by ones who produce it and the ones who read it.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-10-24T18:46:15Z No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) / Made available in DSpace on 2018-10-24T18:46:15Z (GMT). No. of bitstreams: 1 2013 - Gisele Santos de Meireles.pdf: 16741114 bytes, checksum: 1d4b03cbd2287a0984257f70efb149da (MD5) Previous issue date: 2013-02-27 / CAPES / FAPERJ / This study aimed to determine from the protein profile of oocysts of Cystoisospora felis recovered from the sequential use of various purification techniques adapted for specific use in sporulated oocysts of C. felis. With the aid of SDS-PAGE 12% resulted in identification of 25 groups of protein: 266, 240, 186, 165, 140, 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 kDa belonging to the structure of sporulated oocysts and sporozoites of C. felis. Based on this results and heterologous bovine serum anti-C. felis was possible to determine polypeptides dominant relevant to diagnostic immunoassay technique with "Western Blotting", these being immunodominant bands: P208, P138, P113, p106, p62, p56, p51, p48, p44, p38, p36, and p33 p27. In order to avoid misdiagnosis from cross-reactivity a positive control serum anti-C. felis was compared to with positive serum anti-Toxoplasma and Neospora in order to exclude the common protein bands, probably markers of gender and group to identify cattle infected naturally or experimentally with C. felis. As showed, the following specific antigenic protein units: p 206-208, P137-139, p112- 113, p104-107, p27-28 are responsible for determining the animal tested positive or not for Cystoisospora felis. Of the analysis of the variables could be observed that the presence of felines related to the handling, size and type of milking properties facilitates dispersion C. felis. / Este trabalho teve por objetivo, diagnosticar a infec??o por Cystoisospora felis em bovinos atrav?s do Western Blotting, apartir de oocistos obtidos com o uso sequencial de v?rias t?cnicas de purifica??o adaptadas para o uso em oocistos esporulados de C. felis. Com o aux?lio do SDS-PAGE a 12 % resultou na identifica??o de 25 grupos proteicos de: 266; 240; 186; 165; 140; 119, 112, 105, 98, 90, 78, 55, 47, 42, 37, 35, 30, 27-28, 25, 22, 19, 18, 16, 14 KDa, pertencentes a estrutura dos oocistos esporulados e esporozoitas de C. felis. Com base nesse resultado e em soro de bovino heter?logo anti-C. felis foi poss?vel determinar os polipept?deos dominantes relevantes ? t?cnica de diagn?stico imunoenzimatico com ?Western Blotting?, sendo estas, as bandas imunodominantes: p208, p138, p113, p106, p62, p56, p51, p48, p44, p38, p36, p33 e p27. A fim de evitar o diagn?stico equivocado a partir de rea??es cruzadas foi feita a compara??o do soro controle positivo anti-C. felis com o soro positivo anti-Toxoplasma e Neospora com o intuito de excluir as bandas proteicas comuns, prov?veis marcadoras de g?nero e grupo para identifica??o de bovinos infectados de maneira natural ou experimental com C. felis. Sendo evidenciadas, as seguintes unidades proteicas antig?nicas espec?ficas: p 206-208, p137-139, p112-113, p104-107, p27-28 respons?veis por determinar a positividade dos animais testados para C. felis. A partir das an?lises das vari?veis foi poss?vel observar que a presen?a de felinos associados, ao manejo, tipo de ordenha e tamanho das propriedades facilita a dispers?o de C. felis.