Today a lot of attention is focused on compounds called endocrine disrupters (EDs) among substances released to environment by humans. They are a group of substances which can disturb function of hormonal system of organisms including humans. Their poor removal at wastewater treatment plants (WwTP) were shown at various studies, thus they can reach the environment in water. A prospective way for the degradation of EDs at WwTP can be their removal by ligninolytic fungi. They are able to degrade lots of lignin-like aromatic substances because of their highly nonspecific enzymes. In this work growth and enzyme production capability of four ligninolytic fungal strains were monitored on three solid substrates (straw pellets, poplar sawdust mixed with straw pellets, oak sawdust with straw pellets), which may be suitable substrates for fungal growth in bioreactors for wastewater treatment. Ability of these enzymes to degrade EDs were tested in in-vitro degradation experiment. Trametes versicolor was found as best degrading strain with 20 μg/ml of bisphenol A, 17 α- ethynylestradiol and nonylphenol degraded below a quantification limit within 24 hours. Fungal strains degraded EDs well on all of the three substrates but wood sawdust seemed to be a better substrate for fungal growth because straw pellets...

L’intégration 3D est la solution technologique la plus prometteuse pour suivre le niveau d’intégration dictée par la loi de Moore (cf. more than Moore, versus more Moore). Elle entraine des travaux de recherche importants depuis une douzaine d’années. Elle permet de superposer différents circuits et composants dans un seul boitier. Son principal avantage est de permettre une association de technologies hétérogènes et très spécialisées pour la constitution d’un système complet, tout en préservant un très haut niveau de performance grâce à des connexions très courtes entre ces différents circuits. L’objectif de ce travail est de fournir des modélisations cohérentes de via traversant, ou/et de contacts dans le substrat, avec plusieurs degrés de finesse/précision, pour permettre au concepteur de haut niveau de gérer et surtout d’optimiser le partitionnement entre les différentes strates. Cette modélisation passe par le développement de plusieurs vues à différents niveaux d’abstraction: du modèle physique au modèle « haut niveau ». Elle devait permettre de répondre à différentes questions rencontrées dans le processus de conception :- le modèle physique de via basé sur une simulation électromagnétique 2D ou 3D (solveur « éléments finis ») est utilisé pour optimiser l’architecture du via (matériaux, dimensions etc.) Il permet de déterminer les performances électriques des via, notamment en haute fréquence. Les simulations électromagnétiques permettent également de quantifier le couplage entre via adjacents. - le modèle compact analytique de via et de leur couplage, basé sur une description de type ligne de transmission ou noyaux de Green, est utilisé pour les simulations au niveau bloc, ainsi que des simulations de type Spice. Les modèles analytiques sont souvent validés par rapport à des mesures et/ou des modèles physiques. / The 3D integration is the most promising technological solution to track the level of integration dictated by Moore's Law (see more than Moore, Moore versus more). It leads to important research for a dozen years. It can superimpose different circuits and components in one box. Its main advantage is to allow a combination of heterogeneous and highly specialized technologies for the establishment of a complete system, while maintaining a high level of performance with very short connections between the different circuits. The objective of this work is to provide consistent modeling via crossing, and / or contacts in the substrate, with various degrees of finesse / precision to allow the high-level designer to manage and especially to optimize the partitioning between the different strata. This modelization involves the development of multiple views at different levels of abstraction: the physical model to "high level" model. This would allow to address various issues faced in the design process: - The physical model using an electromagnetic simulation based on 2D or 3D ( finite element solver ) is used to optimize the via (materials, dimensions etc..) It determines the electrical performance of the via, including high frequency. Electromagnetic simulations also quantify the coupling between adjacent via. - The analytical compact of via their coupling model, based on a description of transmission line or Green cores is used for the simulations at the block level and Spice type simulations. Analytical models are often validated against measurements and / or physical models.

In recent years, there has been growing interests on II-VI semiconductor nanostructures, which are suitable for applications in electronics and optoelectronic devices such as solar cells, UV lasers, sensors, light emitting diodes and field emission displays. II-VI semiconductor nanostructures with different morphologies such as wires, belts, rods, tubes, needles, springs, tetrapods, plates, hierarchical structures and so on, have been widely grown by vapor transport methods. However the process conditions used for the growth of nanostructures still remains incompatible for device fabrication. The realization of practical nanoscale devices using nanostructured film depends mainly on the availability of low cost and lower processing temperatures to manufacture high purity nanostructures on a variety of substrates including glass and polymer. In this thesis work, studies have been made on the growth and characterization of II-VI semiconductor nanostructures prepared at room temperature, under high vacuum, without employing catalysts or templates. (i) ZnO nanostructured films with different morphology such as flowers, needles and shrubs were deposited at room temperature on glass and polymer substrates by plasma assisted reactive process. (ii) Zn/ZnO core/shell nanowires were grown on Si substrates under optimized oxygen partial pressure. Annealing of this core shell nanowire in high vacuum resulted in the formation of ZnO nanocanals. (iii) ZnS and ZnSe nano and microstructures were grown on Si substrates under high vacuum by thermal evaporation. The morphology, structural, optical properties and composition of these nano and microstructures were investigated by XRD, SEM, TEM, Raman, PL and XPS. The growth mechanism behind the formation of the different nanostructures has been explained on the basis of vapour-solid (VS) mechanism.

Ziel der Arbeit ist die theoretische Analyse von Kohlenstoff-Clustern der Größe 100 - 500 Atome. Die experimentellen Beobachtungen sind bei dieser geringen Anzahl der Atome schwierig. Anderseits sind Kenntnisse über solche Cluster sehr wichtig, z.B. für die Keimbildung von Diamant auf Substraten, oder für die Kohlenstoff-Nanotechnologie (Fullerene, Nanotubes), oder für strukturelle Defekte in Kohlenstoff-Systemen. Es wurden gekrümmte Grenzflächen im Diamant simuliert. Zuerst mit einem empirischen Potential. Es wurde danach eine Methode entwickelt, bei der die schwach gestörten Gebiete einem empirischen Potential gehorchen, und die stark gestörten Gebiete, wo eine genaue Berechnung erforderlich ist, durch eine quantenmechanische Näherung beschrieben wurden. Somit kann man mit guter Genauigkeit große Systeme, bestehend aus einigen 10 (hoch)4 Atomen, simulieren, bei denen nur lokal quantenmechanische Methoden erforderlich sind. Mit diesem Hybrid-Code wurden weiterhin Diamantkeime auf Silizium gerechnet. Es wurden Aussagen bezüglich der Stabilität des Diamants auf dem Siliziumsubstrat, der kritischen Keimgröße, der Änderungen, die der Keim erfährt, gemacht. Ein anderes Gebiet ist die molekulardynamische Simulation bezüglich der Stabilität und des Transformationsverhaltens von Kohlenstoff-Nanoteilchen. Es wurden als »Rohstoffe» sowohl Diamant- und Graphitkristalle sphärischer, ellipsoidischer oder quadratischer Form benutzt, als auch amorpher Kohlenstoff. Es wurde demonstriert, daß sich Diamant unter höherer Temperatur und Bestrahlung in Kohlenstoffzwiebeln transformiert. Es wurde der innere Kern, bestehend aus zwei Schalen, der Kohlenstoffzwiebel simuliert. Es wurde, nach meinem Wissen, zum ersten Mal gezeigt, daß zwischen den Schalen der Kohlenstoffzwiebel Quer-Verbindungen (cross-links) existieren. Diese waren von den Experimentatoren vermutet worden. Sie bilden die Initiatoren der Diamantkeime der Kohlenstoffzwiebel bei ihrer ohne äußeren Druck möglichen Transformation in Diamant. Die Zentren der Kohlenstoffzwiebeln befinden sich bereits in der Entstehung der Zwiebel unter einem Selbstdruck. Bei den größeren Kohlenstoffzwiebeln beträgt der experimentell bestimmte Abstand zwischen den Schalen von außen nach innen von 3.34 Å bis 2.2 Å. Anlagen: nano1.mpg (91,8 MB); nano2.mpg (131 MB) Nutzung: Referat Informationsvermittlung der SLUB / The scope of this work is the analysis of carbon clusters of about 100 - 500 atoms. The experimental studies are at such small clusters heavy. Knowledges about thus clusters are very important, for example in the field of the nucleation of diamond on substrates, or for the carbon nano-technology (fullerene, nanotubes), or for local defects in carbon systems. There were simulated curved interfaces in diamond. Firstly with an empirical potential. Than I developed a method, in wich the defects and the structure around them are treated by a quantum mechanical algorithm and the rest with a near to ideal structure with an empirical potential. So, it is possible an accurate calculation of great systems of about 10 (high)4 atoms on wich only locally quantum mechanical methods are necessary. With this hybrid-code diamond nuclei on silicon substrate were simulated. The stability of the diamond nuclei on the silicon substrate, the critical radius of the nuclei and the changes of the nuclei during his transformation was investigated. Another field of investigations is the molecular dynamics simulation of free carbon clusters. The initial structures had spherical, ellipsoidical or square form and consists of diamond and graphite or a free form in the case of amorphous carbon. It was demonstrated that diamond transforms at higher temperatures and under irradiation in carbon onions. The genesis of the nucleus of a carbon onion with two shells was here for the first time simulated. The existence of the cross-links between the shells of a carbon onion was demonstrated. These existence was expected from the experimentators. The cross-links are the initiators of the transformation of carbon onions to diamond. The center of carbon onions is under self-pressure, because the distance between the outer shells is about 3.34 Å and between the inner shells about 2.2 Å. Appendix: nano1.mpg (91,8 MB); nano2.mpg (131 MB) Usage: Referat Informationsvermittlung/ SLUB

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Made available in DSpace on 2014-12-17T14:55:19Z (GMT). No. of bitstreams: 1 GustavoAC_TESE.pdf: 3178455 bytes, checksum: bdea1ce583a318f3a35fb4f3221877a8 (MD5) Previous issue date: 2014-04-28 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The use of flexible materials for the development of planar circuits is one of the most desired and studied characteristics, lately, by researchers. This happens because the flexibility of the substrate can provide previously impracticable applications, due to the rigidity of the substrates normally used that makes it difficult to fit into the circuits in irregular surfaces. The constant interest in recent years for more lighter devices, increasingly more compacts, flexible and with low cost, led to a new line of research of great interest from both academic and technological views, that is the study and development of textile substrates that can be applied in the development of planar circuits, for applications in the areas of security, biomedical and telecommunications. This paper proposes the development of planar circuits, such as antennas , frequency selective surfaces (FSS) and planar filters, using textile (cotton ticking, jeans and brim santista) as the dielectric substrate and the Pure Copper Polyester Taffeta Fabric, a textile of pure copper, highly conductive, lightweight and flexible, commercially sold as a conductive material. The electrical characteristics of textiles (electric permittivity and loss tangent) were characterized using the transmission line method (rectangular waveguide) and compared with those found in the literature. The structures were analyzed using commercial software Ansoft Designer and Ansoft HFSS, both from the company Ansys and for comparison we used the Iterative Method of Waves (WCIP). For the purpose of validation were built and measured several prototypes of antennas, planar filters and FSS, being possible to confirm an excellent agreement between simulated and measured results / A utiliza??o de materiais flex?veis para o desenvolvimento de circuitos planares ? uma das caracter?sticas mais desejadas e estudadas, ultimamente, pelos pesquisadores, pois essa maleabilidade do substrato proporciona aplica??es antes imposs?veis, devido ? rigidez dos substratos normalmente utilizados o que dificultava a adequa??o dos circuitos em superf?cies irregulares. O constante interesse nos ?ltimos anos por dispositivos mais leves, cada vez mais compactos, flex?veis e com custo reduzido, levou a uma nova linha de pesquisa de grande interesse tanto do ponto de vista acad?mico quanto tecnol?gico que ? o estudo e desenvolvimento de substratos t?xteis que possam ser aplicados no desenvolvimento de circuitos planares, para aplica??es nas ?reas de seguran?a, biom?dica e telecomunica??es. Este trabalho prop?e o desenvolvimento de circuitos planares, tais como antenas, superf?cies seletivas de frequ?ncia (FSS) e filtros planares, utilizando tecidos (lona, jeans e brim santista) como substrato diel?trico e o tecido Pure Copper Polyester Taffeta Fabric, um tecido de cobre puro, altamente condutivo, leve e flex?vel, comercialmente vendido como material condutivo. As caracter?sticas el?tricas dos tecidos (permissividade el?trica e tangente de perda) foram determinadas utilizando o m?todo de linha de transmiss?o e comparadas com os encontrados na literatura. As estruturas foram analisadas utilizando os softwares comerciais Ansoft Designer, Ansoft HFSS ambos da empresa Ansys e para efeito de compara??o foi utilizado o M?todo Iterativo das Ondas (WCIP). Para efeito de valida??o foram constru?dos e medidos v?rios prot?tipos de antenas, FSS e filtros planares sendo poss?vel constatar uma excelente concord?ncia entre os resultados simulados e medidos

Porous, conductive metallic nanostructures are required in several fields, such as energy conversion, low-cost sensors etc. This thesis reports on the development of an electrocatalytically active and conductive membrane for use in Polymer Electrolyte Membrane Fuel Cells (PEMFCs) and fabrication of low-cost substrates for Surface Enhanced Raman Spectroscopy (SERS). One of the main challenges facing large-scale deployment of PEMFCs currently is to fabricate a catalyst layer that minimizes platinum loading, maximizes eletrocatalytically active area, and maximizes tolerance to CO in the feed stream. Modeling the kinetics of platinum catalyzed half cell reactions occurring in a PEMFC using the kinetic theory of gases and incorporating appropriate sticking coefficients provides a revealing insight that there is scope for an order of magnitude increase in maximum current density achievable from PEMFCs. To accomplish this, losses due to concentration polarization in gas diffusion layers, which occur at high current densities, need to be eliminated. A novel catalyst design, based on a porous metallic nanostructure, which aims to overcome the limitations of concentration polarization as well as minimize the amount of platinum loading in PEMFCs is proposed. Fabrication steps involving controlled in-plane fusion of self-assembled arrays of core-shell gold-platinum nanoparticles (Au@Pt) is envisioned. The key steps involved being the development of a facile synthesis route to form Au@Pt nanoparticles with tunable platinum shell thicknesses in the 5 nm size range, the formation of large-scale 2D arrays of Au@Pt nanoparticles using guided self-assembly, and optimization of an RF plasma process to promote in-plane fusion of the nanoparticles to form porous, electrocatalytically active and electrically conductive membranes. This thesis consists of seven chapters. The first chapter provides an introduction into the topic of PEMFCs, some perspective on the current status of research and development of PEMFCs, and an outline of the thesis. The second chapter provides an overview on the methods used, characterization techniques employed and protocols followed for sample preparation. The third chapter describes the modelling of a PEMFC using the Kinetic theory of gases to arrive at an estimate of the maximum feasible current density, based on the kinetics of the electrocatalytic reactions. The fourth chapter presents the development of a simple protocol for synthesizing Au@Pt nanoparticles with control over platinum shell thicknesses from the sub monolayer coverage onwards. The results of spectroscopic and microscopic characterization establish the uniformity of coating and the absence of secondary nucleation. Chapter five describes the formation of a nanoporous, electrocatalytically active membrane by self-assembly to form bilayers of 2D arrays of Au@Pt nanoparticles and subsequent fusion using an RF plasma based process. The evolution of the electrocatalytic activity and electrical conductivity as a function of the duration of RF plasma treatment is monitored for Au@Pt nanoparticles with various extent of platinum coating. Spectroscopic, microscopic, electrical and cyclic voltammetry characterization of the samples at various stages were used to understand the structural evolution with RF plasma treatment duration and discussed. Next durability studies were carried out on the nanoporous, Au@Pt bilayer nanoparticle array with an optimum composition of Pt/Au atomic ratio of 0.88 treated to 16 minutes of argon plasma exposure. After this the novel catalyst membrane design of PEM fuel cell is revisited. Two different techniques are proposed so that the thin, nanoporous, metallic catalyst membrane achieves horizontal electronic resistance equivalent to that of the conventional gas diffusion layer with catalyst layer. The first technique proposes the introduction of gold coated polymeric mesh in between the thin, nanoporous, metallic catalyst membrane and bipolar plate and discusses the advantages. Later the gold coated polymeric mesh is introduced in a conventional membrane electrode assembly and efficiency of the polarization curves probed with and without the introduction of gold coated polymeric mesh. The second technique describes the results of fabrication of a nanoporous metallic membrane using multiple layers of 2D Au@Pt nanoparticle arrays at an optimum composition of Pt/Au atomic ratio of 0.88 to reduce the horizontal electronic resistance. Preliminary studies on the permeability of water through such membranes supported on a porous polycarbonate filter membrane are also presented. In chapter six, a simple reactive inkjet printing process for fabricating SERS active silver nanostructures on paper is presented. The process adapts a simple room temperature protocol, using tannic acid as the reducing agent, developed earlier in our group to fabricate porous silver nanostructures on paper using a commercial office inkjet printer. The results of SERS characterization, spectroscopic and microscopic characterizations of the samples and the comparison of the substrate’s long-term performance with respect to a substrate fabricated using sodium borohydride as the reducing agent is discussed. Preliminary findings on attempts to fabricate a conductive silver network using RF plasma induced fusion area also presented. Chapter seven provides a summary of the results, draws conclusions and a perspective on work required to accomplish the goals of incorporating the porous metallic nanostructures into PEMFCs.

Man’s desire to replicate/mimic the nature’s creation provided an impetus and inspiration to the rapid advancements and progress made in the sensors and actuators technology. A normal human being has five basic sensory organs, which helps and guides him in performing the routine tasks. This underlines the importance of basic sensory organs in a human life. In a similar fashion, sensors and actuators are of paramount importance for most of the science and engineering applications. The aim of the present thesis work is to explore the application of piezoelectric ZnO thin films deposited on a flexible substrate for the development of sensors and actuators. Detailed study was performed on the suitability of three different flexible substrates namely Phynox, Kapton and Mylar. However, Phynox alloy substrate was found to be a suitable substrate material for the above mentioned applications. Sputtering technique was chosen for the deposition of ZnO thin films on to Phynox substrate. The necessary process parameters were optimized to achieve good quality piezoelectric thin films. In the present work, sensors have been developed by utilizing the direct piezoelectric effect of ZnO thin films deposited on Phynox alloy substrate. These includes a flow sensor for gas flow rate measurement, impact sensor for non-destructive material discrimination study and a Thin Film Sensor Array (TFSA) for monitoring the impact events. On the other hand, using the converse piezoelectric effect of ZnO thin films, actuators have also been developed. These include a thin film micro actuator and a Thin Film Micro Vibrator (TFMV) for vibration testing of micro devices. The thesis is divided into following seven chapters. Chapter 1: This chapter gives a general introduction about sensors and actuators, piezoelectric thin films, flexible substrates, thin film deposition processes and characterization techniques. A brief literature survey of different applications of piezoelectric thin films deposited on various flexible substrates in device development is presented. Chapter 2: A novel flexible metal alloy (Phynox) and its properties along with its applications are discussed in this chapter. ZnO thin films were deposited on Phynox substrate by Rf reactive magnetron sputtering technique. The sputtering process parameters such as: Ar:O2 gas ratio, substrate temperature and RF power were optimized for the deposition of good quality piezoelectric ZnO thin films. The deposited ZnO thin films were characterized using XRD, SEM, AFM and d31 coefficient measurement techniques. Chapter 3: It reports on the comparative study of properties of piezoelectric ZnO thin films deposited on three different types of flexible substrates. The substrate materials employed were a metal alloy (Phynox), polyimide (Kapton), and polyester (Mylar). Piezoelectric ZnO thin films deposited on these flexible substrates were characterized by XRD, SEM, AFM and d31 coefficient measurement techniques. A vibration sensing test was also performed for the confirmation of good piezoelectric property. Compared to the polymer flexible substrates, the metal alloy flexible substrate (Phynox) was found to be more suitable for integrating ZnO thin film for sensing applications. Chapter 4: The development of a novel gas flow sensor for the flow rate measurement in the range of L min-1 is presented in this chapter. The sensing element is a Phynox alloy cantilever integrated with piezoelectric ZnO thin film. A detailed theoretical analysis of the experimental set–up showing the relationship between output voltage generated and force at a particular flow rate has been discussed. The flow sensor is calibrated using an in-house developed testing set-up. Chapter 5: This chapter is divided into two sections. Section 5.1 reports on the development of a novel packaged piezoelectric thin film impact sensor and its application in non-destructive material discrimination studies. Different materials (Iron, Glass, Wood and Plastic) were successfully discriminated by using the developed impact sensor. The output response of impact sensor showed good linearity and repeatability. The impact sensor is sensitive, reliable and cost-effective. Section 5.2 reports on the development of a Thin Film Senor Array (TFSA) for monitoring the location and magnitude of the impact force. The fabricated TFSA consists of evenly distributed ZnO thin film sensor array. Chapter 6: It consists of two sections. Section 6.1 reports on the fabrication of micro actuator using piezoelectric ZnO thin film integrated with flexible Phynox substrate. A suitable concave Perspex mounting was designed for the actuator element. The actuator element was excited at different frequencies for the supply voltages of 2V, 5V and 8V. The developed micro actuator has the potential to be used as a micro pump for pumping nano liters to micro liters of fluids. Section 6.2 reports the design and development of a portable ready to use Thin Film Micro Vibrator (TFMV). The TFMV is capable of providing the vibration amplitude in the range of nanometer to micrometer. A thin silicon diaphragm was used as a test specimen for its vibration testing studies using the developed TFMV. The TFMV is light-weight and have internal battery, hence no external power supply is required for its functioning. Chapter 7: The first section summarizes the salient features of the work presented in this thesis. In the second section the scope for carrying out the further work is given.

Yarrowia lipolytica a la particularité de pousser sur de nombreux substrats hydrophobes et de les métaboliser via la β-oxydation ou de les stocker dans des corps lipidiques. Le premier contact entre la cellule et les substrats hydrophobes se déroule sur la surface cellulaire. A cette étape, la composition de la paroi cellulaire en protéines, en β-glucane et en chitine pourrait jouer un rôle important sur l’adhésion et la prise en charge des substrats hydrophobes. Dans le cadre de cette thèse, nous avons étudié l’effet d’une source de carbone hydrophobe (par rapport au glucose) et de la composition du milieu de culture sur la structure pariétale et la production de mélanine chez Y. lipolytica. Les résultats de nos analyses biochimiques et moléculaires ont montré que l’oléate de méthyle (un composé utilisé comme modèle de source de carbone hydrophobe) a induit des modifications au niveau de la paroi cellulaire et que ces modifications ont conduit ou non à l’adhésion de gouttelettes lipidiques à la surface cellulaire ; à une modification du contenu des composés pariétaux ; à la résistance des cellules à des composés toxiques ayant pour cible la paroi cellulaire ; et à la modification des profils d’expression de 27 gènes analysés codant des protéines de paroi cellulaire. L’identité des gènes surexprimés suggère la participation de leurs protéines dans l’intégrité de la paroi cellulaire, dans l’adhésion des gouttelettes lipidiques et dans la réponse au stress par l’oléate de méthyle et/ou le manque d’azote. Nos résultats ont aussi montré que la présence d’oléate de méthyle, huile de ricin et ricinoléate de méthyle a induit la production de DHN-mélanine chez une souche génétiquement modifiée de Y. lipolytica (MTLY40-2p) dont la β-oxydation est affectée / Yarrowia lipolytica can grow on many hydrophobic substrates and metabolize them via the β-oxidation pathway or store them into lipid bodies. The first contact between the cell and the hydrophobic substrates is by the cell wall. In this step, cell-wall proteins (CWP), β-glucane and chitin of the cell wall could play an important role for the adhesion and uptake of hydrophobic substrates. The aim of this work was to study the effect of a hydrophobic carbon source (compared with glucose) and the culture medium composition on the cell wall composition and the melanin production by Y. lipolytica. The results of our biochemical and molecular analysis showed that the presence of methyl oleate and the nutrients composition of the culture media have induced some modifications at the cell wall level. These modifications were linked with the adhesion or not of the lipid droplets to the cell surface; with a modified content of the cell wall components; with a resistance to compounds that are toxic for the cell wall, and with the modification of the expression patterns of 27 CWP genes analyzed in this work. The identity of the over-expressed CWP genes suggests the participation of their proteins in the cell wall integrity, in the adhesion of lipid droplets and in the stress response to methyl oleate and/or the nitrogen starvation. Our results have also shown that the presence of the lipids methyl oleate, castor oil and methyl ricinoleate induced the production of DHN-melanin by a mutant strain in which the β-oxidation is affected / Yarrowia lipolytica tiene la capacidad de crecer sobre numerosos substratos hidrofóbicos y demetabolizarlos a través de la β-oxidación o de almacenarlos en cuerpos lipídicos. El primercontacto entre la célula y los substratos hidrofóbicos se lleva a cabo mediante la pared celular.En esta etapa, la composición en proteínas, β-glucano y quitina de la pared celular podríajugar un papel importante en la adhesión y la toma de substratos hidrofóbicos. En el presentetrabajo se estudió el efecto de una fuente de carbono hidrofóbica (en comparación conglucosa) y la composición del medio de cultivo sobre la composición de la pared celular y laproducción de melanina en Y. lipolytica.Los resultados de nuestros análisis bioquímicos y moleculares mostraron que el oleato demetilo (un compuesto utilizado como fuente de carbono hidrofóbica) indujo modificacionesen la pared celular y estas modificaciones condujeron al aumento o la disminución de laadhesión de gotas lipídicas a la superficie celular; a la modificación del contenido de loscompuestos de la pared celular; a la resistencia de las células a compuestos tóxicos que tienencomo blanco la pared celular; y a la modificación del perfil de expresión de 27 genes quecodifican para proteínas putativas de la pared celular. La expresión de algunos de estos genesse indujo desde las primeras horas de cultivo en presencia de oleato de metilo. La identidad delos genes sobre-expresados sugiere la participación de sus proteínas en mecanismos como laintegridad de la pared celular, la adhesión de las gotas lipídicas a la superficie celular y larespuesta a estrés inducido por oleato de metilo y/o por la falta de nitrógeno en el medio.Nuestros resultados también mostraron que la presencia de oleato de metilo, de aceite dericino y de ricinoleato de metilo en el medio de cultivo, indujo la producción de un pigmentocafé en una cepa mutante de Y. lipolytica (MTLY40-2p) afectada en la β-oxidación. Ademásobservamos que el perfil espectroscópico la pared celular de la mutante MTYL40-2p, semodifica en función del substrato hidrofóbico presente en el medio de cultivo. Nuestrosresultados sugieren que el pigmento café es DHN-melanina y que la ausencia de peptona en elmedio de cultivo afecta la biosíntesis de esta melanina. A partir de un análisis in silicoproponemos los genes putativos para la biosíntesis de DHN-melanina en Y. lipolytica