As the rate of the murder of Black trans women at the hands of Black cisgender men rises steadily every year (HRC, 2017), discourses regarding the detrimental impact of toxic masculinity within Black communities continue to increase within different branches of feminist literature. However, the role that Black cisgender women and toxic femininity play in the violent and systematic subjugation of Black trans women is largely ignored in feminist literature. In this thesis, I conduct a cultural analysis of the representations of the Black trans character Cotton Brown (from the Fox show Star) to examine how the show illustrates toxic femininity and complex intersections of race, class, gender, and sexuality. Through a cultural analysis and review of current literature, I bridge the gap between the representation of cultural politics in Star, literature regarding the same cultural politics, and the realities of the lives of Black trans women in the United States. I argue that Black cisgender women and toxic femininity play significant roles in sociocultural understandings of sexuality and gender identity within Black American communities, as well as the facilitation of violent transphobia that specifically targets Black trans women.

A biofilm, which is associated with 80% of chronic infections in humans, is formed when bacteria aggregate, attach to a substrate and secrete a matrix protecting the bacteria from host cell defenses and antibiotics. Bartonella henselae (B. henselae) is the causative agent of cat scratch disease, persistent bacteremia, and one of the most frequently reported causes of blood-culture negative endocarditis (BCNE) in patients. The ability of B. henselae to adhere to the heart valve, form a biofilm and vegetation to cause endocarditis increases the morbidity and mortality rate in infected patients. The presence of a trimeric autotransporter adhesin (TAA) called Bartonella adhesin A (BadA) has been linked to biofilm formation in B. henselae. BadA is a protein of 3036 amino acids and a member of the TAAs found in Bartonella and other Gram-negative bacteria. The function of BadA has been studied in vitro and is critical for agglutination, host cell adhesion and activation of a pro-angiogenic host response. However, very little is known about badA gene regulation or the molecular basis of biofilm formation. This work aims to determine whether BadA is necessary for the establishment of biofilms and how the bacteria regulate badA expression. Using genetic mutations, real-time cell adhesion assay, RT-qPCR, and microscopy, it was shown that BadA is required for biofilm formation. Using an in-frame complete deletion strain of badA, a reduced ability to form a biofilm was observed which was restored in the deletion strain complemented with a partial badA. Analysis of the B. henselae transcriptome shows nine highly transcribed, homologous RNAs, termed Bartonella regulatory transcript (Brt1-9). The Brts are short-sized (<200 >nucleotides), highly expressed, and located in an intergenic region indicative of small RNAs (sRNA). The Brts are predicted to form a stable stem and loop structure with a potential terminator/riboswitch region on the 3′ end. Located ~20 nucleotides downstream of each Brt is a poorly transcribed helix-turn-helix DNA binding protein gene termed transcriptional regulatory protein (trps 1-9). High brt transcription stops just before the start of the trp implicating the 3’ loop of the Brt as a terminating loop. Replacement of the trp with a gfp reporter gene shows that in the absence of the 3′ end of Brt1, gfp is transcribed. Also consistent with our findings, an increase in both the transcription of trp1 and badA and the formation of a biofilm in mutants of the brt1 gene was observed. Furthermore, to determine the role of the Trp in regulating badA, an electrophoretic mobility shift assay was carried out. The data confirms that Trp1 binds the promoter region of badA gene to regulate gene expression. In summary, the brt1/trp1 regulon affects badA transcription and biofilm formation in B. henselae. Understanding the mechanism and condition(s) by which the brt/trp regulatory system regulates badA is a plausible approach to the development of treatments that target the formation of biofilm-related diseases and persistent bacteremia in humans.

Antalet diagnoser av könsdysfori (KD), har ökat markant i Sverige och andra delar av västvärlden sedan 2008. Merparten av denna ökning utgörs av flickor i tonåren, vilka numera utgör den största gruppen av transpersoner. Tidigare studier av denna till synes nya population baseras på rapporter från föräldrar, som kan vara icke-representativa på flera olika sätt. För att undvika en del av dessa problem rekryterades skolpersonal, som fick besvara en enkät om antalet transidentifieradeelever som de känner till, och hur många som förekommer i samma klass eller kamratgrupp. Hypoteserna var att (1) transpersoner i åldrarna 13- 20 år är födda som flickor i högre utsträckning än som pojkar, (2) att fler än en transidentifierad elev återfinns i samma klassrum eller sociala krets inom en skola är vanligare än förväntat av slumpen, och (3) detta är vanligare bland flickor än pojkar. Enkäten annonserades via Facebooksidor och via e-post till rektorer för svenska grund- och gymnasieskolor. Den besvarades av 196 personer, som rapporterade om 176 transpersoner i 103 olika skolor. Av dessa var 103 flickor (58.5%) och 73 pojkar (41.5%), vilket är en signifikant skillnad. 12 transpersoner var samtidiga i samma klass, 24 i samma kamratgrupp, och 72 i samma skola. Som mest gick fyra transpersoner i samma skola under samma tid. I inget klassrum eller kamratgrupp rapporterades fler än två transpersoner. Utifrån dessa antal kunde inga definitiva slutsatser dras men vårt sampel indikerade att KD inte är medierad inomkamratgrupper. / A significant increase of gender dysphoria (GD) diagnosis has been reported in Sweden and the rest of the West since 2008. The majority of the increase is attributed to biological girls, 13-20 years, who have superseded biological boys in the amount of GD diagnosis. Studies of this seemingly new population were based on parentreports, which can be considered as non-representative for various reasons. To avoid some of those issues school-personnel were recruited as respondents. Three hypotheses were tested; (1) transpersons 13-20 are born as girls at a higher rate than boys, (2) more than one transidentified student in a classroom or social circle in a school is more common than expected by chance, (3) if 2 differs between the sexes it ́s more common amongst those born as girls than those born as boys. The survey posed questions regarding the quantity of transpersons respondents encountered, how many of those were in the same classroom. It was posted on Facebook- pages and emailed to principals of Swedish elementary- and upper secondary schools. 196 persons responded and reported 176 transpersons in 103 different schools. Girls were overrepresented, 103 (58.5%) versus 73 boys (41.5%). Of the 176 reported transpersons, 72 attended a schoolwith at least one other transperson. 12 were part of the same classroom, 24 of the same friendgroup. No more than 4 attended the same school. No class or friendgroup consisted of more than two. No definitive conclusions were made but our sample indicated that GD was not mediated by friendshipgroups.

Peptidyl-prolyl isomerization is an important post-translational modification of protein because proline is the only amino acid that can stably exist as cis and trans, while other amino acids are in the trans conformation in protein backbones. This makes prolyl isomerization a unique mechanism for cells to control many cellular processes. Isomerization is a rate-limiting process that requires a peptidyl-prolyl cis/trans isomerase (PPIase) to overcome the energy barrier between cis and trans isomeric forms. Pin1, a key PPIase in the cell, recognizes a phosphorylated Ser/Thr-Pro motif to catalyze peptidyl-prolyl isomerization in proteins. The significance of the phosphorylation-dependent Pin1 activity was recently highlighted for isomerization of ATR (ataxia telangiectasia- and Rad3-related). ATR, a PIKK protein kinase, plays a crucial role in DNA damage responses (DDR) by phosphorylating hundreds of proteins. ATR can form cis or trans isomers in the cytoplasm depending on Pin1 which isomerizes cis-ATR to trans-ATR. Trans-ATR functions primarily in the nucleus. The cis-ATR, containing an exposed BH3 domain, is anti-apoptotic at mitochondria by binding to tBid, preventing activation of pro-apoptotic Bax. Given the roles of apoptosis in many human diseases, particularly cancer, we propose that cytoplasmic cis-ATR enables cells to evade apoptosis, thus addicting cancer cells to cis-ATR formation for survival. But in normal DDR, a predominance of trans-ATR in the nucleus coordinates with a minimal level of cytoplasmic cis-ATR to promote DNA repair while preventing cell death; however, cells can die when DNA repair fails. Therefore, a delicate balance/equilibrium of the levels of cis- and trans-ATR is required to ensure the cellular homeostasis. In this review, we make a case that this anti-apoptotic role of cis-ATR supports oncogenesis, while Pin1 that drives the formation of trans-ATR suppresses tumor growth. We offer a potential, novel target that can be specifically targeted in cancer cells, without killing normal cells, to significantly reduce the adverse effects usually seen in cancer treatment. We also raise important issues regarding the roles of phosphorylation-dependent Pin1 isomerization of ATR in diseases and propose areas of future studies that would shed more understanding on this important cellular mechanism.

Das Präfix 'trans-' ist aus dem Lateinischen hergeleitet und bedeutet 'jenseits'. Bezogen auf Geschlecht deutet trans* auf Lebensweisen hin, die nicht in einer (vermeintlich natürlichen und angeborenen) Zweigeschlechtlichkeit aufgehen. Transgeschlechtlichkeit wurde in aktivistischen Zusammenhängen in Abgrenzung zu der medizinisch-psychologisch geprägten Kategorie Transsexualität entwickelt. Seit den späten 1960er Jahren eröffnete sich, vor dem Hintergrund ethnomethodologischer Theoriebildung, ein Feld der sozialwissenschaftlichen Untersuchung transgeschlechtlicher Lebensweisen. Im Gegenzug dazu perspektivieren die Queer- und Gender Studies Transgeschlechtlichkeit in den 1990er Jahren neu.

Das Präfix 'trans-' ist aus dem Lateinischen hergeleitet und bedeutet 'jenseits'. Bezogen auf Geschlecht deutet trans* auf Lebensweisen hin, die nicht in einer (vermeintlich natürlichen und angeborenen) Zweigeschlechtlichkeit aufgehen. Transgeschlechtlichkeit wurde in aktivistischen Zusammenhängen in Abgrenzung zu der medizinisch-psychologisch geprägten Kategorie Transsexualität entwickelt. Seit den späten 1960er Jahren eröffnete sich, vor dem Hintergrund ethnomethodologischer Theoriebildung, ein Feld der sozialwissenschaftlichen Untersuchung transgeschlechtlicher Lebensweisen. Im Gegenzug dazu perspektivieren die Queer- und Gender Studies Transgeschlechtlichkeit in den 1990er Jahren neu.

No description available.

Comparison of the observed and calculated vibrational frequencies of all-trans-octatetraene indicates that the density functional theory (DFT) using Becke's exchange and Lee-Yang-Parr's correlation functionals is as accurate as the Hartree-Fock (HF)-based scaled quantum mechanical force field approach in predicting fundamental vibrational frequencies. As the DFT calculation does not use any empirical parameters pertaining to the subject molecule and its computational cost scales more favorably than that of the HF theory, it is a more promising approach to molecular vibrational problems and should replace the empirical normal coordinate analysis for assisting vibrational assignments.

The trans and gender-nonconforming (TGNC) community has a complex relationship with psychiatry. The need for access to transition-related medical services is complicated by an increasing amount of activism that refuses the pathologization of TGNC identities through the diagnosis of Gender Dysphoria and the rejection of the biomedical model of mental illness more broadly. TGNC activists have mobilized concepts from critical disability studies and Mad studies, namely the biomedical and social models of mental illness, to describe their aversion to, and proposals against pathologization. However, this binary relationship between the biomedical and social models is problematic, as it is increasingly evident that conceptualizing TGNC mental health within this binary does not account for the complex reality of the lives of trans and gender-nonconforming people who must navigate between fighting pathologization without sacrificing access to publicly funded transition-related medical procedures, counselling services, and disability benefits. Consequently, in this thesis, I seek to trouble the binary relationship between the biomedical and the social, pointing to the shortcomings of mainstream disability discourses within TGNC mental health policies and practices in Ontario, using Foucault’s notion of biopower and Pamela Moss’ perching model to trace both the consequences of, and alternatives to, these limiting conceptualizations.

The underlying goal of neuroscience research is to understand how the nervous system functions to bring about behavior. A detailed map of neural circuits is required for scientists to tackle this question. To this purpose, we developed a synthetic and genetically-encoded system, TRanscellular ACtivation of Transcription (TRACT) to monitor cell-cell contact. Upon ligand-receptor interaction at sites of cell-cell contact, the transmembrane domain of an engineered Notch receptor is cleaved by intramembrane proteolysis and releases a fragment that regulates transcription in the receptor-expressing cell. We demonstrate that in cultured cells, the synthetic receptor can be activated to drive reporter gene expression by co-incubation with ligand-expressing cell or by growth on ligand-coated surfaces. We further show that TRACT can detect interactions between neurons and glia in the Drosophila brain; expressing the ligand in spatially-restricted subsets of neurons leads to transcription of a reporter in the glial cells that interact with those neurons. To optimize TRACT for neural tracing, we attempted to target the synthetic receptor to post-synaptic sites by fusion with the intracellular domain of Drosophila neuroligin2. However, this modification only facilitate the receptor to be localized homogeneously throughout the neurites. The induction data of the modified receptor shows that the new receptor has better sensitivity compared to the original receptor, but the ligand-receptor interaction still happened at non-synaptic sites of membrane contact. To further target the ligand to pre-synaptic sites, we fused the ligand to different pre-synaptic markers. We found the one fused with synaptobrevin is likely located at axon terminals, but only able to trigger moderate induction. Therefore, more examinations are required to further characterize the capability of this ligand. In summary, TRACT is useful for monitoring cell-cell interactions in animals and could also be used to genetically manipulate cells based on contact. Moreover, we believe that proper targeting of the ligand to synaptic sites will improve the specificity of TRACT for synaptic connections in the future.