Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-04-10T18:37:45Z No. of bitstreams: 1 1993 - Gilberto Salles Gaz?ta.pdf: 930210 bytes, checksum: 0c425531817e903d01966c5e960cb65b (MD5) / Made available in DSpace on 2018-04-10T18:37:45Z (GMT). No. of bitstreams: 1 1993 - Gilberto Salles Gaz?ta.pdf: 930210 bytes, checksum: 0c425531817e903d01966c5e960cb65b (MD5) Previous issue date: 1993-07-19 / CNPq / Two hundred and four females and one hundred and twenty males of Anocentor nitens (Neumann, 1897), obtained of equines from 12 districts in the Southern part of Brazil were analyzed morphologically concerning its peritremal plate and festoons, that are taxonomics parameters utilized for diagnosis of this species. The number of peritremal plate should variation with predominant asymmetry. Variations observed in the peritremal plate of males and females of A. nitens suggest the formation of cline in Minas Gerais State going to Rio de Janeiro State and S?o Paulo State. The number of festoons in males and females of the species analyzed was equal to the one characterized in the diagnosis of A. nitens / Duzentos e quatro f?meas e cento e vinte machos de Anocentor nitens (Neumann, 1897), oriundos de equ?deos localizados em 12 munic?pios da regi?o Sudeste, foram analisados morfologicamente quanto aos caracteres placa peritrem?ticas e fest?es, par?metros taxon?micos utilizados na diagnose desta esp?cie. As varia??es ocorridas nas Placas Peritrem?tica de macho e f?meas de A. nitens, sugerem a forma??o de ?cline? a partir do Estado de Minas Gerais, em dire??o ao Estado do Rio de Janeiro e ao Estado de S?o Paulo. O n?mero de fest?es dos esp?cimes analisados foi igual, em machos e f?meas, ?quele caracterizados na diagnose de A. nitens.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2018-05-10T19:41:52Z No. of bitstreams: 1 2015 - Aline Braga Moreno.pdf: 3503961 bytes, checksum: 9ee745976fac8dfd56f36e7a0d024468 (MD5) / Made available in DSpace on 2018-05-10T19:41:52Z (GMT). No. of bitstreams: 1 2015 - Aline Braga Moreno.pdf: 3503961 bytes, checksum: 9ee745976fac8dfd56f36e7a0d024468 (MD5) Previous issue date: 2015-02-25 / CAPES / FAPERJ / Instituto Oswaldo Cruz / Previous studies on the helminth fauna of scombrid fish from the Brazilian coast included reports on Scomber japonicus Houttuyn, 1782 and Scomber colias Gmelin, 1789. Nowadays its considered that only S. colias occurs in the Atlantic Ocean, Mediterranean Sea and Black Sea, forming different populations. To assess and revise the parasite biodiversity of S. colias off the coast of Rio de Janeiro State, 69 fish from Public Markets of S?o Pedro da Aldeia and Serop?dica were collected and analyzed, from March 2013 to November 2014. Fish were examined and all organs were individualized in Petri dishes containing saline solution 0.7% to be analyzed under the stereo microscope. The parasites were fixed in 70% etanol, AFA or 4% formalin. The Nematoda were cleared and examined in temporary mounts with glycerin and Monogenea, Trematoda and Cestoda were stained with Semichon's acid carmine or Gomori's trichrome and examined as permanent mounts in Canada balsam. The taxonomic identification was made by measuring with the ocular micrometer, by illustrations using a camera clara, and by studies on scanning electron microscopy and molecular techniques. The prevalence, intensity amplitude, mean abundance and standard deviation were calculated per each parasite species. Parasite community study was made at the infracomunity level using the data of 50 fish collected in a single sample from S?o Pedro da Aldeia, which represents a component community. The mean richness, mean total abundance and the mean of Berger-Parker dominance index, mean Bray-Curtis similarity and the mean of Brillouin diversity index of the infracommunities were also calculated. Additionally, using the SIMPER procedure, were also identified the species that contributed most to the similarity between the infracommunities. We collected 578 parasites belonging to 9 taxa: Monogenea (Grubea cochlear and Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis and Allonematobothrioides scombri - new host record and new geographical distribution), Cestoda (Scolex pleuronectis) and Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larvae). Raphidascaris sp. was the most prevalent (64%), followed by K. scombri (60%) and A. laguncula (38%). The most abundant species was K. scombri (2.30% ? 3.73). The mean infracommunity richness was 2.14 ? 1.09 with a total mean abundance of 7.08 ? 7.24. The mean Berger-Parker dominance index was 0.67 ? 0.27, K.scombri, Raphidascaris sp. and A.laguncula being the most dominant species. The Bray-Curtis similarity index between infracommunities was relatively low (32.78 ? 26.20) and Raphidascaris sp. and Hysterothylacium sp. were contributing most to the similarity. / Os trabalhos pr?vios sobre a helmintofauna de peixes escombr?deos da costa brasileira inclu?ram refer?ncias em Scomber japonicus Houttuyn, 1782 e Scomber colias Gmelin, 1789. Hoje se considera que apenas S. colias ocorre no Oceano Atl?ntico, Mar Mediterr?neo e Mar Negro, onde forma popula??es distintas. Com o objetivo de avaliar e revisar a biodiversidade parasit?ria de S. colias, do litoral do Estado do Rio de Janeiro, foram coletados e analisados 69 peixes provenientes de Mercados P?blicos dos Munic?pios de S?o Pedro da Aldeia e Serop?dica, no per?odo de Mar?o de 2013 a Novembro de 2014. Os peixes foram necropsiados e todos os ?rg?os foram individualizados em placas de Petri com solu??o salina 0,7% para serem examinados no microsc?pio estereosc?pico. Os parasitos foram fixados em ?lcool a 70%, AFA ou formalina a 4%. Os Nematoda foram diafanizados e examinados em l?minas tempor?rias com glicerina e os Monogenea, Trematoda e Cestoda corados em Carmim de Semichon ou Tricr?mico de Gomori e examinados em l?minas permanentes montadas em B?lsamo do Canad?. A identifica??o taxon?mica foi feita atrav?s de medidas com aux?lio de ocular microm?trica, desenhos em c?mara clara, estudos por microscopia eletr?nica de varredura e t?cnicas moleculares. Foram calculadas a preval?ncia, amplitude de intensidade, abund?ncia m?dia e desvio padr?o para cada esp?cie de parasito. Os estudos de comunidades se realizaram a n?vel de infracomunidade com os dados de 50 peixes coletados em uma ?nica amostra em S?o Pedro de Aldeia, que representa uma comunidade componente. Foram tamb?m calculadas a riqueza m?dia, a abund?ncia total m?dia, ?ndice m?dio de domin?ncia de Berger-Parker e os ?ndices m?dios de similaridade de Bray-Curtis e de diversidade de Brioullin. Adicionalmente, utilizando o procedimento SIMPER, foram avaliadas as esp?cies que mais contribu?ram para a similaridade entre as infracomunidades. Foram coletados 578 parasitos, pertencentes a 9 taxa: Monogenea (Grubea cochlear e Kuhnia scombri), Digenea (Aponurus laguncula, Lecithocladium harpodontis e Allonematobothrioides scombri ? novo hospedeiro e nova distribui??o geogr?fica), Cestoda (Scolex pleuronectis) e Nematoda (Raphidascaris sp., Hysterothylacium sp., Anisakidae gen. sp. larva). Raphidascaris sp. apresentou maior preval?ncia (64%), seguido por K. scombri (60%) e A. laguncula (38%). A esp?cie mais abundante foi K. scombri (2,30% ? 3,73). A riqueza m?dia de esp?cies a n?vel de infracomunidade foi de 2,14?1,09 com uma abund?ncia total de 7,08?7,24. As infracomunidades apresentaram um ?ndice de domin?ncia de Berger-Parker de 0,67?0,27 sendo as esp?cies mais dominantes K.scombri, Raphidascaris sp. e A.laguncula. O ?ndice de similaridade de Bray-Curtis entre as infracomunidades foi relativamente baixo (32,78?26,20) e as esp?cies Raphidascaris sp. e Hysterothylacium sp. foram as que mais contribu?ram para a similaridade.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-31T19:48:45Z No. of bitstreams: 1 1998 - Anthony ?rico da Gama Guimar?es.pdf: 2433104 bytes, checksum: 69d8edd6924e8b5f41e776f896b1b385 (MD5) / Made available in DSpace on 2017-05-31T19:48:45Z (GMT). No. of bitstreams: 1 1998 - Anthony ?rico da Gama Guimar?es.pdf: 2433104 bytes, checksum: 69d8edd6924e8b5f41e776f896b1b385 (MD5) Previous issue date: 1998-09-03 / CNPq / Faperj / In order to analyze some aspects related to the ecology of mosquitoes (Diptera: Culicidae) in areas of the State Park of the Serra do Mar, State of Sao Paulo, monthly captures were accomplished, using human bait, in three different periods of the day, in wild and domiciliary environments and 24 consecutive months, from January, 1991 to December, 1992. In 622 samplings, 24.943 specimens of adult mosquitoes belonging to 61 species were captured. Highest frequencies were due to Cq. chrysonotum with 45,8% of the total of captured mosquitoes, followed by: Ae. serratus with 6,8%, Cq. venezuelensis with 6,5%, Ps. ferox with 5,2 and Ps. albipes with 3,1%. Monthly averages of temperature and relative humidity of the air were inserted in the secular maximum and minimum medium limits of the 10 previous years and the one of rainfall accompanied the curve of the averages of secular tendency of the same period. Those climatic factors were decisive for the incidence of some species; temperature: An. cruzii, An. mediopunctatus, Ae. scapularis, Ae. fulvus, Cq. chrysonotum, Cq. venezuelensis, Ru. reversa, Wy. dyari, Wy. confusa, Wy. shannoni, Ph. theobaldi and Li. flavisetosus; relative humidity: Ae. serratus, Ae. scapularis, Cq. venezuelensis and Ru. reverse; rainfall: An. cruzii, Ae. scapularis, Ae. fulvus, Cq. venezuelensis Ru. reverse, Ph. theobaldi and Li. flavisetosus. Some species were identified according to certain capture stations. Cq. chrysonotum and Cq. venezuelensis demonstrated preference for the swamp areas and puddles found in the stations A and B. An. mediopunctatus, Cx. nigripalpus, Ae. serratus, Ae. fulvus, Ps. ferox, Ps. albipes and the Sabethinae in general were captured almost exclusively in wild areas, especially in the stations B and C. The domicile located in the station D, was preferred by An. cruzii, An. oswaldoi and An. fluminensis. However, Cx. quinquefasciatus was the only eusynanthropic species. An. cruzii and Ae. scapularis were captured accomplishing the blood repast in the intra and peridomicile, indicating that both species, vectors of etiologic agents responsible for malaria and arboviroses respectively , in other places in the coast of the State of Sao Paulo, can be involved in the domiciliary transmission of those diseases. Except for the incidences of Tr. digitatum in the night period, the Sabethinae were captured essentially in the illuminated hours of the day. All the Anophelinae were preferably in the night samplings and the Culicinae presented nocturnal species: Cx. nigripalpus, Cq. chrysonotum and Cq. venezuelensis; of the day: Ps. ferox and Ps. albipes; and eclectic as the preference for the occurrence period: Ae. serratus, Ae. scapularis and Ae. fulvus. The lunar phases were not decisive to the incidence of the Culicidae fauna. However, the samplings accomplished in nights of new moon were more profitable quantitatively. Ae. scapularis was captured preferably in nights of full moon. / Visando conhecer aspectos da ecologia de mosquitos (Diptera: Culicidae) em ?reas do Parque Estadual da Serra do Mar, Estado de S?o Paulo, foram realizadas capturas mensais sistematizadas, em isca humana, em tr?s diferentes per?odos do dia, em ambiente silvestre e domiciliar e ao longo de 24 meses consecutivos, de janeiro de 1991 a dezembro de 1992. Em 622 amostragens, foram capturados 24.943 esp?cimes de mosquitos adultos pertencentes 61 esp?cies. As maiores ocorr?ncias ficaram por conta de Cq. chrysonotum com 45,8% do total de mosquitos capturados, seguida por: Ae. serratus ? 6,8%, Cq. venezuelensis = 6,5%, Ps. ferox ? 5,2 e Ps. albipes ? 3,1%. As m?dias mensais de temperatura e umidade relativa do ar estiveram inseridas nos limites das m?dias seculares de m?xima e m?nima dos 10 anos anteriores e as de precipita??es pluviom?tricas acompanharam a curva das m?dias de tend?ncia secular do mesmo per?odo. Esses fatores clim?ticos foram determinantes para incid?ncia de algumas esp?cies; temperatura: An. cruzii, An. mediopunctatus, Ae. scapularis, Ae. fulvus, Cq. chrysonotum, Cq. venezuelensis, Ru. reversa, Wy. dyari, Wy. confusa, Wy. shannoni, Ph. theobaldi e Li. flavisetosus; umidade relativa: Ae. serratus, Ae. scapularis, Cq. venezuelensis e Ru. reversa; precipita??es pluviom?tricas: An. cruzii, Ae. scapularis, Ae. fulvus, Cq. venezuelensis Ru. reversa, Ph. theobaldi e Li. flavisetosus. Algumas esp?cies estiveram identificadas com determinadas esta??es de captura. Cq. chrysonotum e Cq. venezuelensis demostraram prefer?ncia pelas ?reas de mangue e charcos encontradas nas esta??es A e B. An. mediopunctatus, Cx. nigripalpus, Ae. serratus, Ae. fulvus, Ps. ferox, Ps. albipes e os sabet?neos em geral foram capturados quase que exclusivamente em ?reas silvestres, especialmente nas esta??es B e C. O domic?lio localizado na esta??o D foi preferido pelo An. cruzii, An. oswaldoi e An. fluminensis. Entretanto, Cx. quinquefasciatus foi a ?nica esp?cie verdadeiramente eussinantr?pica. An. cruzii e Ae. scapularis foram capturadas realizando o repasto sang??neo no intra e peridomic?lio, indicando que ambas as esp?cies, vetoras de agentes etiol?gicos causadores respectivamente de mal?ria e arboviroses em outras localidades no litoral do Estado de S?o Paulo, podem estar envolvidas na transmiss?o domiciliar dessas doen?as. Excetuando-se as incid?ncias de Tr. digitatum no per?odo noturno, os sabet?neos foram capturados essencialmente nas horas iluminadas do dia. Todos os anofelinos estiveram preferencialmente nas amostragens noturnas e os culic?neos apresentaram esp?cies noturnas: Cx. nigripalpus, Cq. chrysonotum e Cq. venezuelensis; diurnas: Ps. ferox e Ps. albipes; e ecl?ticas quanto a prefer?ncia pelo per?odo de ocorr?ncia: Ae. serratus, Ae. scapularis e Ae. fulvus. As fases lunares n?o foram determinantes ? incid?ncia da fauna culicideana. Entretanto, as amostragens realizadas em noites de lua nova foram quantitativamente mais proveitosas. Ae. scapularis foi capturado preferencialmente em noites de lua cheia.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-08-02T16:42:16Z No. of bitstreams: 1 2010 - Victor do E. Santo Cunha.pdf: 1746605 bytes, checksum: 28c67099d77713106e9e71f40c014b18 (MD5) / Made available in DSpace on 2016-08-02T16:42:16Z (GMT). No. of bitstreams: 1 2010 - Victor do E. Santo Cunha.pdf: 1746605 bytes, checksum: 28c67099d77713106e9e71f40c014b18 (MD5) Previous issue date: 2010-10-21 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, CAPES. / Whole-body extracts and proteins of mites are used for the diagnosis of allergic diseases in humans and animals. The objective of the present study was to evaluate the biological activity of commercial Dermatophagoides farinae and Blomia tropicalis allergenic extracts in dogs with atopic dermatitis and to identify the allergens present in these extracts by serological assays. For this purpose, 26 dogs were submitted to skin tests using commercial mite extracts (ALK-Abell? or FDA Allergenic) and skin reactions were evaluated after 15 min. Allergenic proteins present in the D. farinae and B. tropicalis extracts were identified by immunoblotting using sera from allergic animals and anti-dog IgE conjugate. The results showed that 0.95 and 1.68 BU/mL of the commercial D. farinae and B. tropicalis extracts (ALK-Abell?), respectively, are necessary to provoke a papule measuring 14 mm in diameter in susceptible animals. Immunoblotting analysis of antigens present in the D. farinae extract (FDA Allergenic) using sera from 10 allergic animals showed that 80% of the sera recognized a band of approximately 102 kDa, 80% two bands of 52 to 76 kDa, 70% two bands larger than 225 kDa, 50% one band of approximately 76 kDa, 50% one band of approximately 225 kDa, 40% one band of 31 to 38 kDa, and 20% one band of 12 to 17 kDa. Immunoblotting of antigens of the B. tropicalis extract (FDA Allergenic) showed that 50% of the sera recognized two bands of 52 to 76 kDa. These results demonstrate the importance of the two house dust mite species for the pathogenesis of canine atopic dermatitis in Brazil. In addition, the results indicate allergens that should be present in allergenic extracts used for diagnosis and allergenspecific immunotherapy. / CUNHA, Victor do Espirito Santo. Estudo da atividade biol?gica de extratos de corpo total de ?caros da poeira domiciliar em c?es com dermatite at?pica e identifica??o sorol?gica de al?rgenos. 2010. 49p Tese (Doutorado em Ci?ncias Veterin?rias, Sanidade Animal). Instituto de Veterin?ria, Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2010. Extratos de corpo total e prote?nas de ?caros s?o utilizados para diagn?stico de doen?as al?rgicas em seres humanos e animais. O objetivo do presente trabalho foi determinar a atividade biol?gica de extratos alerg?nicos comerciais de Dermatophagoides farinae e Blomia tropicalis em c?es com dermatitite at?pica e identificar sorologicamente al?rgenos presentes nesses extratos. Para tanto, 26 c?es foram testados intradermicamente com extratos comerciais de ?caros (ALK-ABELL? ou FDA Allergenic) e as rea??es cut?neas avaliadas ap?s 15 minutos. Prote?nas alerg?nicas presentes nos extratos de D. farinae e B. tropicalis foram identificadas por ?immunoblotting? utilizando-se soros dos animais al?rgicos e conjugado anti-IgE canina. Os resultados obtidos mostraram que, para provocar uma p?pula com 14 mm de di?metro nos animais sens?veis, s?o necess?rios 0,95 BU/mL e 1,68 BU/mL de extratos comerciais de D. farinae e B. tropicalis (ALK-ABELL?), respectivamente. A an?lise por ?immunoblotting? dos ant?genos presentes no extrato de D. farinae (FDA Allergenic), utilizando soros de dez animais al?rgicos, mostrou que 80% dos soros reconhecem uma banda com peso molecular de aproximadamente 102 kDa; 80% duas bandas entre 52 e 76 kDa; 70% duas bandas acima de 225 kDa; 50% uma banda com aproximadamente 76 kDa; 50% uma banda com aproximadamente 225 kDa; 40% uma banda entre 31 e 38 kDa; e 20% uma banda entre 12 e 17 kDa. A an?lise por ?immunoblotting? dos ant?genos do extrato de B. tropicalis (FDA Allergenic) mostrou que 50% dos soros reconhecem duas bandas com pesos moleculares entre 52 e 76 kDa. Esses resultados demonstram a import?ncia dessas duas esp?cies de ?caros da poeira domiciliar na patog?nese da dermatite at?pica canina no Brasil, assim como indicam al?rgenos que devem estar presentes nos extratos alerg?nicos utilizados para diagn?stico e imunoterapia al?rgeno-espec?fica.

Made available in DSpace on 2016-04-28T20:17:26Z (GMT). No. of bitstreams: 1 2006-Felipe Lopes Campos.pdf: 553352 bytes, checksum: e4d71e5d911a1305af8111fa2c150966 (MD5) Previous issue date: 2006-03-16 / The aim of this survey was the isolation of the Cryptococcus neoformans yeast from cats and dogs central nervous system in Rio de Janeiro City. Periodic collections were made from brain samples from April 2004 to July 2005 at the rabies diagnosis laboratory in the Jorge Vaitsman Veterinary Medicine Municipal Institute in Mangueira, RJ. According to the results, of the 201 clinical samples, we found four positives samples; one of these was positive for C. laurentii, three were positive for Cryptococcus neoformans; of which two were neoformans variety and one was gattii variety. Secondarily we compared with strains from the ICB-USP, previously isolated and identified as for urease and fenol-oxidase production in qualitative character, in the same way that the potential of virulence demonstrated to fosfolipase and protease production. Killer biotyping used in all isolates showed a predominance of biotype II to neoformans variety and biotype VIII to gattii variety. All isolates showed protease and fosfolipase production in varied degrees. lipase / Objetivando o isolamento da levedura Cryptococcus neoformans do sistema nervoso central de c?es e gatos no Munic?pio do Rio de Janeiro, procedeu-se ?s coletas peri?dicas, no per?odo de abril de 2004 a julho de 2005, de amostras de c?rebro, no Laborat?rio de Diagn?stico de Raiva do Instituto Municipal de Medicina Veterin?ria Jorge Vaitsman no bairro da Mangueira, Rio de Janeiro. Das 201 amostras obteve-se um total de quatro positivas para o g?nero; sendo uma de Cryptococcus laurentii e tr?s de C. neoformans, das quais duas revelaram-se pertencentes ? variedade neoformans e uma ? variedade gattii. Secundariamente, confrontamos estas com amostras obtidas junto ao ICB-USP, previamente isoladas e identificadas, quanto ao potencial de virul?ncia espelhado pela produ??o de fosfolipase e de protease. A biotipagem Killer empregada em todos os isolados demonstrou uma predomin?ncia do biotipo II para a variedade neoformans e biotipo VIII para a variedade gattii. Todos os isolados apresentaram-se produtores de protease e de fosfolipase, em diferentes graus.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-07-29T12:59:33Z No. of bitstreams: 1 2011 - Tatiana Salom?o Barbosa.pdf: 562866 bytes, checksum: 6cdcd52bb7ab78c9cb4b4a47f918a85c (MD5) / Made available in DSpace on 2016-07-29T12:59:33Z (GMT). No. of bitstreams: 1 2011 - Tatiana Salom?o Barbosa.pdf: 562866 bytes, checksum: 6cdcd52bb7ab78c9cb4b4a47f918a85c (MD5) Previous issue date: 2011-02-09 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior- CAPES. / BARBOSA, Tatiana Salom?o. Mycological and mycotoxicological evaluation of feed supplied in the diet of tilapias in breeding. 2011. 37 p. Dissertation (Master?s Degree in Science and Technology of Food). Technology Institute, Federal Rural University of Rio de Janeiro, Serop?dica, RJ, 2011. Pisciculture is a specific branch of aquaculture aimed at raising fish in captivity. For its great advantages tilapia stand out as the queens of modern pisciculture and its cultivation has been pointed out by experts as a promising activity in the world and especially in Brazil due to its potential hydrological, climatic conditions and excellent satisfactory production of grains used in manufacturing ration. It is known these grains are highly susceptible to fungal contamination with possible formation of mycotoxins, and this constitutes a major problem worldwide. Among the genera evolved in mycotoxins production is Aspergillus, Penicillium e Fusarium. The most prevalent mycotoxins are aflatoxins, ochratoxin A, fumonisins, zearalenone and trichothecenes. These are substances capable of affecting the productivity with serious economic losses and risks to animal and human health. Then, this study aimed to determine the water activity and the total contaminant mycoflora, to establish the occurrence of species potentially mycotoxin-producing belonging to the genera Aspergillus, Penicillium and Fusarium, to determine the presence of aflatoxin B1, ochratoxin A, fumonisin B1 and to characterize mycotoxins profile of toxigenic strains isolated from feed supplied in the diet of tilapia in piscicultures. ,Samples (60) were collected from commercial feed directly from the fish-farming properties in Central and South regions of Rio de Janeiro. Water activity was measured . The total mycoflora was determined by serial decimal dilution plate and fungal colonies were identified at genera level. The identification of Aspergillus spp. and Penicillium spp. followed specific taxonomic keys. The profile was conducted to toxigenic strains potentially ochratoxin A and aflatoxin B1 producers isolated from the samples. The determination of the natural incidence of ochratoxin A and aflatoxin B1 was performed by thin layer chromatography and fumonisin B1 was determined by ELISA kits. Data were submitted to analysis of variance (ANOVA) and comparisons of means by Tukey's test (p < 0.05). The average of the aW of the samples was 0.61 ? 0.041 in the Central Region and 0.58 ? 0.060 in the South, both below the value considered optimal for fungal growth and mycotoxin production. Mean levels of fungal contamination were 1.0 x 103 CFU g-1 in the Central and 4.7 x 103 CFU g-1 in the South region, being within the recommended standards for international Good Manufacturing Practice for feed, which is 1.0 x 104 CFU g-1 The most isolated genera were Cladosporium (85%), Aspergillus (68%) and Penicillium (60%). The most frequent species were P. citrinum (31%), A. niger aggregate (21%) and A. flavus (20%). All isolates of A. niger aggregate were negative for the capacity to produce ochratoxin A. Among the A. flavus strains, 16% were positive for the production aflatoxin B1. Concerning the natural incidence of mycotoxins, the samples had detectable levels of fumonisin B1 (98%), aflatoxin B1 (55%) and ochratoxin A (2%). The detection of mycotoxins in almost all the feed samples analyzed highlights the need to implement good agriculture and manufacturing practices, in order to ensure the safety of feed and to reduce the risk of mycotoxicosis occurrence. / BARBOSA, Tatiana Salom?o. Avalia??o micol?gica e micotoxicol?gica de ra??es fornecidas na dieta de til?pias em criat?rios. 2011. 37 p. Disserta??o (Mestrado em Ci?ncia e Tecnologia de Alimentos). Instituto de Tecnologia, Universidade Federal Rural do Rio de Janeiro, Serop?dica, RJ, 2011. A piscicultura ? um ramo espec?fico da aq?icultura voltada para a cria??o de peixes em cativeiro. Por suas grandes vantagens as til?pias se destacam como as rainhas da piscicultura moderna e seu cultivo vem sendo apontado por especialistas como promissora atividade no mundo e principalmente no Brasil em decorr?ncia do seu potencial hidrogr?fico, condi??es clim?ticas excelentes e produ??o satisfat?ria de gr?os utilizados na fabrica??o de ra??o. Sabese que estes gr?os s?o altamente suscept?veis ? contamina??o f?ngica com poss?vel forma??o de micotoxinas, o constitui um problema de grande import?ncia em n?vel mundial. Dentre os g?neros f?ngicos envolvidos na produ??o de micotoxinas destacam-se o Aspergillus, Penicillium e Fusarium. As micotoxinas de maior preval?ncia s?o as aflatoxinas, ocratoxina A, fumonisinas, zearalenona e tricotecenos. Estas s?o subst?ncias capazes de afetar os par?metros produtivos, com graves perdas econ?micas e riscos para a sa?de animal e humana. Assim, este estudo objetivou determinar a atividade de ?gua e a micobiota total contaminante, estabelecer a ocorr?ncia de esp?cies potencialmente produtoras de micotoxinas pertencentes aos g?neros Aspergillus, Penicillium e Fusarium, determinar a presen?a de aflatoxina B1, ocratoxina A e fumonisina B1 e caracterizar o perfil tox?geno de cepas de esp?cies isoladas de amostras de ra??es fornecidas na alimenta??o de til?pias provenientes de pisciculturas. Para isto, foram coletadas 60 amostras de ra??es comerciais diretamente das propriedades piscicultoras nas regi?es Centro e Sul do Estado do Rio de Janeiro. As amostras tiveram sua atividade de ?gua aferida. Em seguida, a micobiota total foi determinada pelo m?todo de dilui??o decimal seriada em placa. Depois de isoladas, as col?nias foram identificadas em n?vel de g?nero. A identifica??o de Aspergillus spp. e Penicillium spp. seguiu chaves taxon?micas espec?ficas. O perfil tox?geno foi realizado para as cepas potencialmente produtoras de ocratoxina A e de aflatoxina B1 isoladas das amostras. A determina??o da incid?ncia natural destas micotoxinas foi realizada atrav?s de cromatografia em camada delgada. A determina??o da presen?a de fumonisina B1 nas amostras foi feita atrav?s de kits ELISA. Os resultados foram submetidos ? an?lise de vari?ncia (ANOVA) e compara??o de m?dias pelo teste de Tukey (p < 0,05). A m?dia obtida da Aa das amostras foi de 0,61 ? 0,041 na Regi?o Centro e de 0,58 ? 0,060 na Regi?o Sul, ambas abaixo do valor considerado ?timo para o crescimento f?ngico e produ??o de micotoxinas. Os n?veis m?dios de contamina??o f?ngica foram de 1,0 x 103 UFC g-1 no Centro e de 4,7 x 103 UFC g-1 no Sul do Estado, ficando dentro dos padr?es recomendados pelas Boas Pr?ticas de Fabrica??o internacional para a alimenta??o animal, que ? de 1,0 x 104 UFC g-1 de amostra. Os g?neros f?ngicos mais isolados foram Cladosporium (85%), Aspergillus (68%) e Penicillium (60%). As esp?cies mais freq?entes foram P. citrinum (31%), A. niger agregados (21%) e A. flavus (20%). Todas as cepas isoladas de A. niger agregados foram negativas para o teste de capacidade produtora de ocratoxina A. Dentre as cepas de A. flavus isoladas, 16% foram positivas para a produ??o de aflatoxina B1. Quanto ? incid?ncia natural de micotoxinas, 98% das amostras apresentaram n?veis detect?veis de fumonisina B1, 55% de aflatoxina B1 e 2% de ocratoxina A. A detec??o de micotoxinas em quase todas as amostras de ra??o analisadas ressalta a necessidade de implanta??o de boas pr?ticas de agricultura e de fabrica??o, a fim de se garantir um alimento mais seguro e reduzindo os riscos da ocorr?ncia de micotoxicoses.

Submitted by Sandra Pereira (srpereira@ufrrj.br) on 2016-09-19T11:49:34Z No. of bitstreams: 1 2011 - Michel Jos? Sales A. Helayel.pdf: 11350186 bytes, checksum: ab1a4bf6f150ee6e971e58207f995bff (MD5) / Made available in DSpace on 2016-09-19T11:49:34Z (GMT). No. of bitstreams: 1 2011 - Michel Jos? Sales A. Helayel.pdf: 11350186 bytes, checksum: ab1a4bf6f150ee6e971e58207f995bff (MD5) Previous issue date: 2011-03-16 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This study aimed to evaluate the protective effect of acetamid in experimental poisoning by Pseudocalymma elegans in sheep, goats and rabbits, in order to prove indirectly that monofluoroacetate (MF) is responsible for the clinical signs and death of animals that ingested the plant. Experiments were performed to determine for sheep and goats the lethal dose of P. elegans collected in Rio Bonito, RJ, in different seasons, and to adjust the dose of acetamid to be administered. In the first experiment, four animals received 1.0g/kg of fresh P. elegans, and two others were pretreated with 2.0g/kg of acetamid. None of the animals showed clinical signs or died. Possibly, the plant could be less toxic, since it was collected at the end of the rainy season. In the second experiment, two sheep and two goats received 0.67 and 1.0g/kg of the dried plant, after pretreatment with 2.0 and 3.0g/kg of acetamid, respectively. All animals died, as the administered doses of P. elegans were very high. In the third experiment, two sheep and two goats received 0.333g/kg of dried P. elegans after previous administration of 2.0g/kg of acetamid; a week later, the protocol above was repeated, but without the antidote. In experiments with rabbits, doses of 0.5 and 1.0g/kg of dried P. elegans were given after administration of 3.0g/kg of acetamid; seven days later, the same protocol was repeated, except the administration of acetamide. This procedure, when acetamid was administered before, prevented the appearance of clinical signs and death of sheep, goats and rabbits. But the animals not treated with acetamid showed symptoms of poisoning and died. Clinically, the sheep and goats had tachycardia, engorged jugular vein, positive venous pulse, lateral recumbence, and muscle tremors. In the "dramatic phase?, the animals fell into lateral position, stretched the limbs, were paddling and died within minutes. The rabbits showed apathy, muscle tremors, vocalization and lateral decumbence minutes before death. At postmortem examination, the sheep and goats had engorged jugular veins and atria, dilated Vena cava cranialis and caudalis, as well as pulmonary edema, hepatic congestion and edema of the gallbladder subserosa. In rabbits, the main macroscopic alterations were dilated atria, engorged Vena cava cranialis and caudalis, and congested liver and diaphragm vessels. Histopathology revealed, in two sheep and one goat, vacuolar-hydropic degeneration of the distal convoluted kidney tubules, together with caryopicnosis. In the rabbits, the liver showed severe congestion with numerous shock corpuscles. The experimental results show indirectly that MF is to be held responsible for death of the animals that ingested P. elegans; since "acetate donor" compounds, such as acetamid, are capable to reduce the competitive inhibition of MF for the same active site (Coenzyme A) which prevents the formation of fluorocitrate, its active metabolite, formed in the body through the socalled "lethal synthesis". / O presente trabalho teve como objetivo avaliar o efeito protetor da acetamida nas intoxica??es experimentais por Pseudocalymma elegans (Bignoniaceae) em ovinos, caprinos e coelhos, com a finalidade de comprovar indiretamente que o monofluoroacetato ? respons?vel pela sintomatologia e morte dos animais que ingerem essa planta. Foram realizados experimentos para determinar a dose letal da planta coletada em Rio Bonito, RJ, em diferentes ?pocas do ano para ovinos e caprinos e ajustar a dose de acetamida a ser administrada. No primeiro experimento, dois ovinos e dois caprinos receberam 1,0 g/kg de P. elegans fresca e um animal de cada esp?cie foi tratado previamente com 2,0 g/kg de acetamida. Nenhum animal apresentou altera??es cl?nicas ou morreu. Ao que tudo indica a planta poderia estar menos t?xica, j? que foi coletada no fim da esta??o das ?guas. No segundo experimento, dois ovinos e dois caprinos receberam 0,67 e 1,0 g/kg da planta dessecada, ap?s tratamento pr?vio, com 2,0 e 3,0 g/kg de acetamida, respectivamente. Todos os animais morreram, pois administramos doses muito altas de P. elegans. No terceiro experimento, dois ovinos e dois caprinos receberam, 0,333 g/kg de P. elegans dessecada, ap?s administra??o pr?via de 2,0 g/kg de acetamida. Uma semana depois, o protocolo acima foi repetido, por?m sem o ant?doto. Nos experimentos com coelhos, foram administradas doses de 0,5 e 1,0 g/kg de P. elegans dessecada ap?s a administra??o de 3,0 g/kg de acetamida. Sete dias depois, o mesmo protocolo foi repetido, com exce??o da administra??o de acetamida. Esta, quando administrada previamente, evitou o aparecimento dos sinais cl?nicos e a morte dos ovinos, caprinos e coelhos, j? os animais n?o tratados com acetamida apresentaram sintomatologia e morreram. Clinicamente, os ovinos e caprinos manifestaram taquicardia, jugulares ingurgitadas, pulso venoso positivo, dec?bito esternal e tremores musculares. Na ?fase dram?tica?, os animais ca?am em dec?bito lateral, esticavam os membros, faziam movimentos de pedalagem e morriam em poucos minutos. Nos coelhos observaram-se apatia, tremores musculares, dec?bito lateral e vocaliza??o minutos antes da morte. A avalia??o macrosc?pica revelou, nos ovinos e caprinos, jugulares ingurgitadas, aur?culas, veia cava caudal e cranial dilatadas, al?m de edema pulmonar, congest?o hep?tica e edema na subserosa da ves?cula biliar. Nos coelhos as principais altera??es observadas foram aur?culas dilatadas, veia cava caudal e cranial ingurgitadas, f?gado e vasos do diafragma congestos. O exame histopatol?gico revelou, em dois ovinos e um caprino, degenera??o hidr?pico-vacuolar dos t?bulos urin?feros contornados distais associada ? cariopicnose. Nos coelhos havia congest?o hep?tica acentuada com numerosos corp?sculos de choque. Nossos resultados comprovam, de forma indireta, que o MF ? respons?vel pela morte dos animais que ingerem essa planta, uma vez que compostos ?doadores de acetato? como a acetamida, s?o capazes de reduzir a inibi??o competitiva do MF pelo mesmo s?tio ativo (Coenzima A), o que impede a forma??o do fluorocitrato, seu metab?lito ativo, formado no organismo por meio da denominada ?s?ntese letal?.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-02-14T17:34:23Z No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) / Made available in DSpace on 2017-02-14T17:34:23Z (GMT). No. of bitstreams: 1 1997 - Isabelle Garcia Pina.pdf: 529649 bytes, checksum: d067fa62978c5955301ef929db0ee4f8 (MD5) Previous issue date: 1997-09-30 / CNPq / This experimental work with Aedes aegypti L. (1762) was conducted in order to compare the effect of differents blood sources on feeding and egg production of mosquitoes? females. The blood-feeding device consisted of a plastic cage ? used for holding females during blood feeding ? with two lateral windows recovered with thin cloth to allow air exchange during feeding. In the top of the cage, there was an opening where the food?s container, recovered with silicone membrane, was attached. A larger vial filled with water at the initial temperature of 50 ?C was used over the apparatus to keep the food source warm (37,5 ? 0,5%). In each trial, three different treatments were tested: whole blood collected from mouse, bovine and human. To prevent coagulation, a 3,9% solution of sodium citrate was added to the blood. The control group was allowed to feed directly on human volunteers. The experiment was conducted in a climatic chamber, with temperature and humidity controlled at 28?0,5 ?C and 80?5%, respective values and the photoperiod of 12 hours of light and 12 hours of darkness. In each tested group, 30 females and 10 males mosquitoes were used. The females were allowed to feed during a period of 20-30 minutes and after that only the engorged females were transferred to another cages containing the oviposition sites. To evaluate the effect of different sources of blood on A. aegypti, the percentage of engorged females, pre-oviposition time and the number of eggs per engorged female were measured. Highest percentage of engorged females were among mosquitoes fed on human citrated blood (89%) and control (95%). The media oviposition on both treatments were 13,4 eggs for females fed on human citrated blood and 15,3 eggs for females of control group. The lots fed on mice and bovine citrated blood presented the following results respectively: 22,5% of engorged females and 9,2 eggs per female and 25,8% of engorged females and 10,3 eggs per female. / Foi comparado o efeito do sangue de camundongos, bovino e humano sobre o ingurgitamento e oviposi??o do mosquito Aedes aegypti L., 1762 (D?ptera:Culicidae). O aparato para alimenta??o artificial foi constitu?do por gaiolas de pl?stico, com 10 x 6 cm, com orif?cios laterais cobertos com tela de tule, e uma abertura superior, atrav?s da qual foi oferecido o sangue contido em uma c?lula montada em l?mina de microscopia com cola de silicone e membrana de silicone. O sangue foi mantido aquecido a 37,5 ? 0,5 ?C, durante o tempo de alimenta??o que variou de 20 a 30 minutos. O experimento foi conduzido dentro de uma c?mara climatizada, com temperatura de 28 ? 0,5 ?C, umidade relativa de 80 ? 5% e fotoperiodismo de 12 horas di?rias. Um total de 16 grupos de 30 f?meas de A. aegypti, com idades entre 4 a 7 dias, foram pr?-alimentados com solu??o a?ucarada de sacarose. Em cada grupo foram adicionados 10 machos para realiza??o de c?pula. O sangue de cada hospedeiro foi coletado assepticamente e adicionado de citrato de s?dio a 3,9%. O grupo controle foi alimentado diretamente em hospedeiro humano volunt?rio. Ap?s a alimenta??o, as f?meas ingurgitadas foram contadas e colocadas no interior de gaiolas com 50 x 50 x 50 cm, contendo frasco com ?gua limpa para oviposi??o. Foram registrados o n?mero de f?meas alimentadas em cada tratamento, o per?odo de pr?-postura e o n?mero m?dio de ovos por f?mea alimentada. Mosquitos alimentados com sangue de camundongos e de bovino tiveram performances de 22,5 e 25,8% de ingurgitamento e a postura foi em m?dia de 9,2 e 10,3 ovos por f?mea, respectivamente. F?meas alimentadas com sangue humano atrav?s da membrana ou diretamente no hospedeiro humano tiveram comportamentos diferentes com ?ndices de ingurgitamento de 89 e 95% e oviposi??o de, em m?dia, 13,4 e 15,3 ovos por f?mea, respectivamente. N?o houve varia??o no per?odo de pr?-postura (tr?s dias) entre os tratamentos realizados.

Submitted by Leticia Schettini (leticia@ufrrj.br) on 2017-03-17T13:12:34Z No. of bitstreams: 1 2001 - Marilene Alvares.pdf: 614893 bytes, checksum: ca3d3b41579b663ff4afa2939a0a892f (MD5) / Made available in DSpace on 2017-03-17T13:12:34Z (GMT). No. of bitstreams: 1 2001 - Marilene Alvares.pdf: 614893 bytes, checksum: ca3d3b41579b663ff4afa2939a0a892f (MD5) Previous issue date: 2001-08-15 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The habromatids biology and their patogenies have abready been well studied. But not na efficient technique has been developed, or peridically tested for the diagnosis of the gastric habronemiasis in equidae. Has been proposed the compation of two techniques: the UENO (1986) technique and UENO & GUTIERRES (1983), modified and the xenodiagnostic technique. On the Ueno (modified) technique to recuperate first stage larvae, has been used fecal examples of 3g and 5g, with 3 repetitions for each. From each example has been taken 0,3 ml of the sediment, divided in three microscope slides and then the slides were observed though the light microscope for counting the larvae number of Habronema spp. or Drashia megastoma. To the xenodiagnostic, larvae of Musca domestica and Stomoxys calcitrans (Muscidae: Diptera) were inoculated in the equidae feces and then were kept in the laboratory. The adults muscoids0 were dissected and the third stage larvae of the nematodes were saved and counted. The adults nematodes were colected from the stomach of 35 equidae then fixed in AFA and preserved in alcohol 70% for later identification. The comparative analysis were based on the linear correlation. The comparation of the determination rates indicated that there weren?t significative difference on the recuperation of larvae on the Ueno (modified) technique, with the two different examples weights and the xenodiagnostic technique. The Ueno (modified) technique utilizing 3g and 5g of feces had the same index of determination, 73%. The test t analysis showed no significant difference between the three slides on the Ueno technique observation. While the xenodiagnostic technique got a determination rate of 79,1%, when utilizing only M. domestica and the two especies of females of Habronema spp. And the determination index of 81,3% when utilizing M. domestica and females of Habronema muscae based on the linear correlation. / A biologia das Habronemat?neos e suas patogenias j? foi bem estudada. No entanto, nenhuma t?cnica coprosc?pica eficiente foi desenvolvida e rotineiramente testada para o diagn?stico da habronemose g?strica em eq??deos. Foi proposta a compara??o de duas t?cnicas: a t?cnica de UENO (1968) e UENO & GUTIERRES (1983) modificada e a t?cnica do xenodiagn?stico. Na t?cnica de Ueno (modificada) para recupera??o da larva de primeiro est?gio, foi utilizado amostras contendo 3g e 5g de fezes, com tr?s reparti??es para cada amostra de fezes. De cada amostra foi retirado 0,3 ml de sedimento, dividindo em tr?s l?minas sendo levado ao microsc?pio ?tico para contagem do n?mero de larvas de Habronema spp. ou Drashia megastoma. Para o xenodiagn?stico, larvas de Musca domestica e Stomoxys calcitrans (Muscidae: Diptera) foram inoculadas nas fezes dos eq??deos e mantidos em laborat?rio. Os musc?deos adultos foram dissecados e as formas de terceiro est?gio dos nemat?ides recuperados e contados. Os nemat?ides adultos foram coletados do est?mago de 35 eq??deos, fixados em AFA e conservados em ?lcool a 70%, para posterior identifica??o. A an?lise comparativa foi baseada na regress?o linear. A compara??o dos ?ndices de determina??o indicaram n?o haver diferen?a significativa na recupera??o de larvas na t?cnica de Ueno (modificada), com os dois diferentes pesos de amostras de fezes e a t?cnica do xenodiagn?stico. A t?cnica de Ueno (modificada) utilizando 3g e 5g de fezes obteve o mesmo ?ndice de determina??o 73%. A an?lise do teste t demonstrou n?o existir diferen?a significativa entre as tr?s l?minas na leitura da t?cnica de sedimenta??o. Enquanto a t?cnica do xenodiagn?stico obteve o ?ndice de determina??o de 79,1% quando utilizado apenas M. domestica para realiza??o desta, e as duas esp?cies de f?meas de Habronema spp. e o ?ndice de determina??o de 81,3% quando utilizado M. domestica e f?meas de Habronema muscae como base da regress?o linear.

Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-04-11T21:16:25Z No. of bitstreams: 1 2012 - Matheus Dias Cordeiro.pdf: 852495 bytes, checksum: c420562c23340ca737374426f8c5e0f7 (MD5) / Made available in DSpace on 2017-04-11T21:16:25Z (GMT). No. of bitstreams: 1 2012 - Matheus Dias Cordeiro.pdf: 852495 bytes, checksum: c420562c23340ca737374426f8c5e0f7 (MD5) Previous issue date: 2012-02-29 / CNPq / Brazilian Spotted Fever and Baggio-Yoshinari Syndrome are emerging diseasest characterized as the only known to tick-borne zoonoses in Brazil. This study aimed to avaluate IgG antibodies against Borrelia burgdorferi and Rickettsia rickettsii in stray and domiciled dogs from Serop?dica-RJ and studying the fauna of ixodida these animals. To investigate the prevalence of antibodies against R. rickettsii and B. burgdorferi was performed to collect blood from 293 dogs in four areas of the city of Serop?dica-RJ. Serum samples were processed by Indirect Immunofluorescence (IFA) against crude antigens of R. rickettsii. The same animals were tested for IgG antibodies against B. burgdorferi strain of North American origin, using the Indirect Enzyme-linked immunosorbent assay (ELISA). To confirm the presence of Borrelia spp., 102 positive samples were tested by Western Blotting (WB). Ticks were also collected from the inspection of the animals. The specimens found were manually removed and stored in polypropylene bottles, and later identified. The association between seropositive animals and variables, were performed using the chi-square and analysis of variance (ANOVA) with significance level of 5%. Of the 283 sera tested by IFA, 23.67% (67/283) showed reactivity against species-specific antigens of R. rickettsii. Thus, we found a prevalence of 21.11% (19/90) in "40 km", 21.84% (19/87) in "Km 49", 25% (8 / 32) in "54 km" and 28.38% (21/74) in UFRRJ. The titles ranged from 1:64 to 1:512. On the other hand, of the 293 animals studied, 154 (52.56%) were positive for homologous antibodies anti-B. burgdorferi. It was founded a prevalence of 43.75% (14/32) in the "54 km", 51.72% (45/87) in the "49 km", 46.67% (42/90) in the "40 km" and 63.1% (53/84) in UFRRJ. It was no significant difference (p<0.05) between the prevalence of antibodies against Borrelia sp. in stray dogs and domiciled. Dogs with access to the street had a frequency against R. rickettsii significantly (p<0.05) greater than animals kept in prison. There were no statistical differences (p>0.05) the relationship between the variables: presence of ticks, animal sex, habits, frequenting pastures and age of the animals with seropositivity observed in either species. Regarding ticks, at least 64.5% (189/293) dogs were infested with ticks at the moment of collection. Only two species of ticks were identified, Rhipicephalus sanguineus and Amblyomma cajennense. The presence of antibodies anti-R. rickettsii and anti-B. burgdorferi in dogs from Serop?dica-RJ is indicative of the presence of rickettsias of the Rock Mountain Spotted Fever Group and spirochetes dogs in this area. / A Febre Maculosa Brasileira e a S?ndrome de Baggio-Yoshinari s?o enfermidades emergentes, caracterizadas como as ?nicas zoonoses conhecidamente transmitidas por carrapatos, no Brasil. O presente estudo teve como objetivo detectar anticorpos da classe IgG contra Rickettsia rickettsii e Borrelia burgdorferi e estudar a fauna de Ixod?deos nesses animais. Para investigar a preval?ncia de anticorpos contra R. rickettsii e B. burgdorferi foi efetuada a coleta de sangue de 293 c?es, em quatro ?reas do munic?pio de Serop?dica-RJ. Os soros obtidos foram processados atrav?s da Rea??o de Imunofluoresc?ncia Indireta (RIFI), contra ant?genos brutos de R. rickettsii. Os mesmos animais foram submetidos ? pesquisa de anticorpos hom?logos da classe IgG contra B. burgdorferi cepa americana G39/40, utilizando Ensaio de Imunoadsor??o Enzim?tico (ELISA) Indireto. Para confirma??o da presen?a de Borrelia spp. no munic?pio, 102 amostras positivas foram testadas pelo Western Blotting (WB). A fauna de ixod?deos foi estudada atrav?s da coleta de carrapatos a partir da inspe??o das regi?es das orelhas, dorso e coxins palmares e plantares dos animais. Os esp?cimes encontrados foram removidos manualmente e acondicionados em frascos de polipropileno, e posteriormente, identificados. O estudo da associa??o entre animais soropositivos e as vari?veis avaliadas, foram realizados por meio do teste de Qui-quadrado e An?lise de Vari?ncia (ANOVA), com n?vel de signific?ncia de 5%. Dos 283 soros testados ? RIFI, 23,67% (67/283) apresentaram reatividade contra ant?genos esp?cie-espec?fico de R. rickettsii, sendo encontrada uma frequ?ncia de 21,11% (19/90) no ?Km 40?, 21,84% (19/87) no ?Km 49?, 25% (8/32) no ?Km 54? e 28,38% (21/74) no campus da UFRRJ. A titula??o variou entre 1:64 ? 1:512. Por outro lado, dos 293 animais estudados, 154 (52,56%) foram positivos para anticorpos hom?logos anti-B. burgdorferi, sendo encontrada uma frequ?ncia de 43,75% (14/32) no ?Km 54?, 51,72% (45/87) no ?Km 49?, 46,67% (42/90) no ?Km 40? e 63,1% (53/84) na UFRRJ. Houve diferen?a significativa (p<0,05) entre as preval?ncias de anticorpos contra Borrelia spp. encontradas em caninos errantes e domiciliados. Os c?es com acesso a rua tiveram uma frequ?ncia de anticorpos contra R. rickettsii significativamente (p<0,05) maior que animais mantidos preso. N?o foram observadas diferen?as estat?sticas (p>0,05) entre a rela??o das vari?veis: presen?a de carrapatos, sexo dos animais, h?bitos de frequentar pastos e idade dos animais com a soropositividade observada em nenhuma das duas esp?cies. Quanto aos carrapatos, 64,5% (189/293) dos c?es estavam infestados por carrapatos no momento da coleta. Apenas duas esp?cies de carrapatos foram identificadas, Rhipicephalus sanguineus e Amblyomma cajennense. A presen?a de anticorpos hom?logos anti-R. rickettsii e anti-B. burgdorferi em caninos de Serop?dica-RJ ? um indicativo da presen?a de rickettsia do grupo da Febre Maculosa e espiroquetas em c?es nesta ?rea.