Made available in DSpace on 2014-10-09T12:42:17Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:03:00Z (GMT). No. of bitstreams: 0 / Com a intenção de estabelecer métodos físico-químicos como uma alternativa ao bioensaio in vivo para determinação de atividade biológica, o conteúdo de hFSH, hTSH e hLH de diferentes preparações, nativas e recombinantes, foi determinado por cromatografia líquida de alta eficiência em fase reversa (RP-HPLC) e comparado ao dado obtido pelo clássico bioensaio in vivo em camundongos ou ratos (BA). Para estes hormônios foi encontrada uma relação linear entre os dois métodos: hFSH BAUI = 0,9925 RP-HPLCUI - 1,3165, r = 0,9371, p < 0,001, n = 24; hTSH BA&mu;g = 0,9790 RP-HPLC&mu;g - 0,052, r = 0,8725 , p < 0,001, n = 14; hLH BAUI = 0,8771 RP-HPLCUI + 12,41; r = 0,9786, p < 0,01, n = 5. Para outras nove preparações de hFSH e onze preparações de hTSH foi determinada a diferença média (d) entre a bioatividade predita pela RP-HPLC através destas equações e da média das bioatividades obtidas com os dois métodos. Para o hLH não foi possível determinar esta diferença em virtude das poucas amostras disponíveis. No caso do hFSH, d ± DP = -2,11 ± 3,49 % sendo a precisão de 1,16% e no caso do hTSH, d ± DP = -2,01 ± 5,56 % com precisão de 1,68%. Amostras parcialmente alteradas apresentaram diferentes graus de atividade de hFSH, hTSH e hLH que puderam ser preditas por RP-HPLC com uma aceitável concordância com os bioensaios in vivo. Estes resultados demonstraram que o emprego de um ensaio físico-químico sem o uso de animais, tal como a RP-HPLC, é uma alternativa viável ao uso do bioensaio in vivo para a determinação da potência de hFSH e hTSH, reduzindo assim o número de animais em geral utilizados para assegurar a qualidade e eficácia de um produto farmacêutico. / Tese (Doutoramento) / IPEN/T / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP

Os fatores condicionantes antrópicos e morfométricos são determinantes na formação do escoamento pluvial, do manejo do solo e na concentração de fluxos e detritos. No município de São Pedro (SP) é notória a ocorrência desses processos, sendo classificado, segundo o mapa de erosão do Estado de São Paulo como de alta suscetibilidade. Desta forma, a bacia do córrego Espraiado, localizada neste município, foi selecionada com objetivo de analisar a gênese e a distribuição das feições erosivas a partir da influência dos parâmetros morfométricos (curvatura, declividade e hipsometria) e antrópicos numa escala evolutiva (1962, 1972, 2000 e 2006) e também numa análise integrada e quantitativa (feições erosivas, uso do solo e parâmetros morfométricos), por meio do cálculo dos seguintes índices: Concentração de Erosão (CE) e Potencial de Erosão (PE). O primeiro índice é a razão entre o número de células em cada classe afetadas pela erosão e o número total de células na bacia, enquanto o segundo índice é a razão entre o número de células em cada classe afetadas pela erosão e o número total de células na mesma classe. Nos mapas das feições erosivas, observou-se que a partir do cenário de 1962 até 2006 a área total das feições erosivas registrou uma redução, no qual a cabeceira das drenagens revelou o setor com maior ocorrência e concentração de feições. O mapa do uso do solo revelou que o aumento do percentual de mata, da área urbana consolidada e da silvicultura, e a redução do solo exposto e da pastagem tiveram um papel determinante para a diminuição das feições erosivas, embora a classe de área urbana consolidada tenha influenciado diretamente para o surgimento de novas feições e a evolução de outras. A análise integrada mostrou que a curvatura côncava, a declividade entre 20 e 40% e as classes de solo exposto e de pastagens foram as mais suscetíveis e que estão presente principalmente nas áreas das cabeceiras de drenagens, setor que está associado a solos pouco espesso. Os índices também indicaram que mesmo as áreas com a presença de curvatura convexa ou retilínea e declividades entre 0 e 15% com baixos índices de PE mas com alterações antrópicas significativas, obtiveram aumento do potencial de ocorrência de feições erosivas, assim como, as classes de uso com baixo índice de PE quando associados aos parâmetros morfométricos de alto índice, propiciaram aumento da formação de feições erosivas. / The anthropic and morphometric factors are significant in the formation of runoff, soil management and concentration flows and debris. In the São Pedro city (SP) is recognized to exist of these processes, being classified, according to the erosion map of São Paulo state as high susceptibility. Thus, the Espraiado basin, located in this city, was selected to analyze the genesis and distribution of erosional features by the influence of morphometric parameters (curvature, slope and elevation) and anthropic in the evolutionary scale (1962, 1972, 2000 and 2006) and also an integrated and quantitative analysis (erosional features, land use and morphometric parameters), by calculating the following index: Erosion Concentration (EC) and Erosion Potential (EP). The first index is the ratio of the number of cells in each class affected by the erosion areas to the total number of cells in the basin, while the second index is the ratio of the number of cells in each class affected by the areas of erosion to the total number of cells in the same class. In the erosional features maps, we observed from 1962 to 2006 scenario, that the total area of erosional features decrease, in which the head drainages revealed the sector with the highest occurrence and concentration of features. The land use map showed that the increase of the percentages of the forest, of the urban area consolidated and the forestry, and the reduction of exposed soil and pasture were the role played on the reducing the erosive features, although the class of urban area consolidated was directly influenced in the new features and developments of the others. The integrated analysis showed that the concave curvature, the slope between 20 and 40% and the classes of exposed soil and pasture were the most susceptible and are primarily in the headwaters, a sector that is associated with very thick soils. These areas of high concentration runoff flow and debris, with fragile lithology and have a PE high, is related to the presence of curvature concave, in profile and plant, and concentrated flows, as well as high slope that increases the erosive power of the flow, factors that are associated with the erosion formation. The index also indicated that even the areas with the presence of convex or straight curvatures, slopes between 0 and 15%, with low PE but with significant anthropogenic changes, obtained an increase of the potential occurrence of erosional features, as well as classes use with low PE when associated with morphometric parameters of high, caused an increase of erosional features.

Orientador: Helena Maria Andre Bolini / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-08-22T21:24:46Z (GMT). No. of bitstreams: 1 Palazzo_AlessandraBugatte_D.pdf: 3757920 bytes, checksum: c5aacf04a4c0054e8161cba577ed32e0 (MD5) Previous issue date: 2013 / Resumo: Mundialmente a exigência por dietas controladas tem crescido vertiginosamente. Entre elas, destacam-se alimentação com redução calórica, restrição de açúcares, gorduras e sódio, almejando a prevenção de doenças e tratamento de certas deficiências. Em função dessa demanda do mercado, aliada a políticas de incentivo ao avanço tecnológico, buscou-se um alimento que atendesse a essas necessidades. Nesse contexto, verificou-se também a expansão do mercado interno de chocolates, a qual destaca aumento de 14% no consumo de chocolates entre 2009 e 2010, fomentando competitividade e necessidade de inovação neste segmento. Diante disso, o presente projeto visou formular diferentes chocolates dietéticos isentos de lactose, produzidos à base de extrato proteico de soja, utilizando três distintos edulcorantes em substituição à sacarose. Além disso, os mesmos edulcorantes foram incorporados a massas de chocolates ao leite, garantindo dessa forma, um comparativo entre as amostras. Os edulcorantes analisados foram sucralose, rebaudiosídeo e neotame, juntamente com polidextrose e eritritol, como agentes de corpo e agregaores de doçura. As doçuras equivalentes foram determinadas através da análise tempo-intensidade para gosto doce. Visando a caracterização dos produtos formulados foram realizados dois testes sensoriais descritivos. Conduziu-se uma Análise Descritiva Quantitativa com equipe treinada composta de 11 provadores, os quais avaliaram 17 termos descritores durante sessões individuais de análise. No teste tempo-intensidade foram abordados os atributos considerados mais relevantes para análise de chocolate em função do tempo, como os gostos doce e amargo, sabor de chocolate e derretimento na boca. Paralelamente a avaliação afetiva foi conduzida através de teste de aceitação com, no mínimo, 112 consumidores potenciais de chocolates. Os parâmetros relacionados ao processamento dos chocolates também foram controlados, como tamanho máximo de partículas durante o refino da massa e índice de temperagem. Após oito horas de conchagem e resfriamento controlado, os chocolates foram avaliados quanto à viscosidade e textura instrumental. Consolidando a caracterização do produto foi elaborado um estudo de vida útil durante o período de 12 meses, o qual os chocolates permanecerem armazenados em câmara à 23ºC. Dessa forma, justifica-se o estudo voltado ao chocolate, produto este altamente comercializado e apreciado mundialmente por consumidores de diferentes faixas etárias. Através da análise de tempo-intensidade para doçura equivalente, determinaram-se as concentrações dos edulcorantes, sendo 0,075% de sucralose, 0,0215% de rebaudiosídeo, 0,005% de neotame, 17% de polidextrose e 26% de eritritol. Durante a ADQ, os provadores descreveram todos os chocolates semelhantes em relação ao aroma de cacau e aroma de manteiga de cacau. Os resultados de aceitabilidade para os chocolates de soja apontaram aceitação pelos consumidores até 6 meses de estocagem. Ao contrário disso, os chocolates ao leite permaneceram com valores de aceitação entre 4,8 a 6,0 (considerando a escala de 9 pontos) no prazo final de 12 meses de estudo. A sucralose demonstrou semelhança sensorial em relação ao controle, visando à substituição integral da sacarose, evidenciada pela ADQ, tempo-intensidade múltipla e aceitação. Atributos como brilho e homogeneidade contribuíram positivamente na aceitação dos chocolates, verificada pela correlação dos Quadrados Mínimos Parciais entre os chocolates ao leite e de soja em relação aos atributos da ADQ e impressão global do teste de aceitação. Em contrapartida, tem-se aroma de manteiga de cacau como parâmetro que contribui de forma negativa à aceitabilidade pelos consumidores. A redução calórica calculada em torno de 28% em relação a todos os chocolates formulados reforçou a denominação light/diet destes alimentos destinados ao público em questão. Conclui-se assim, que indivíduos que apresentem alterações metabólicas como intolerância à lactose ou diabetes, além de alergias relacionadas às proteínas lácteas, podem ser beneficiados com essas opções de chocolates / Abstract: Worldwide the demand for controlled diets has grown dramatically. Among them, calorie reduction, restriction of sugar, reduction of fat and sodium, targeting disease prevention and treatment of certain deficiencies. According to this market demand, combined with politics to encourage technological advancement, we searched for a food that meets those needs. In this context the expansion of the internal market of chocolates, with 14% of increase in chocolate consumption between 2009 and 2010, together with competitiveness and requeriments for segment¿s innovation. Therefore, the objetive of this project is to formulate different diet lactose free chocolates, produced based on soy protein extract, using four different sweeteners to replace sucrose. Furthermore, the same sweeteners are incorporated in the milk chocolates mass, thereby ensuring a comparison between the samples. The sweeteners analyzed were sucralose, rebaudioside and neotame, and polydextrose and erithritol as bulk agents. The sweetness equivalent was determined by time-intensity analysis for sweet taste. Two descriptive sensory tests were conduced to characterize the formulated products. A Quantitative Descriptive Analysis was done with 11 trained painel, who rated 17 parameters during individual sessions to analyze and describe all the chocolates similar regarding the cocoa flavor and aroma of cocoa butter. The time-intensity analysis have been conduced with the most important attributes for chocolate analysis of time, such as tastes sweet and bitter, chocolate flavor and melting in the mouth. Both an affective evaluation was conducted through the acceptance test with at least 112 potential consumers of chocolates. The processing parameters of chocolates were also controlled, as medium particle size during the refining mass and index value of tempering. After eight hours of conching and controlled cooling, the chocolate were evaluated for viscosity and texture analysis. Complementing the characterization of the product, a study of shelf life was done during 12 months, which the chocolates were stored in the camera 23ºC. The objective of this study was justified considering that chocolate are marketed and highly appreciated worldwide for consumers of different age groups. Through multiple time-intensity for sweet taste, sweetners concentrations were determined, 0,075% to sucralose, 0,0215% to rebaudioside, 0,005% to neotame, 17% to polydextrose and 26% to erithritol. The results of acceptability for chocolate soy showed consumer acceptance until six months of storage. In contrast, the milk chocolates remained with acceptance values between 4,8 to 6,0 (considering 9-point scale) on the deadline of 12 months of study. Sucralose showed similar sensory compared to control, aiming at the replacement of the sucrose, as evidenced by the QDA, multiple time-intensity and acceptance. Attributes as brigthness and homogeneity have positively contributed for chocolate acceptance, realized in Partial Least Square between milk and soy chocolates, QDA attributes and global feeling to acceptance test. On the other hand, cocoa butter aroma as attribute that contributed with negative form to accetpance by consumers. The calculated caloric reduction of around 28% on all chocolates formulated claim the denomination light / diet of these foods to the public in question. Thus, individuals who have metabolic disorders such as lactose intolerance or diabetes, plus related allergies to milk proteins may benefit from these options of chocolate products / Doutorado / Consumo e Qualidade de Alimentos / Doutor em Alimentos e Nutrição

La spectroscopie de plasma induit par laser, ou LIBS (acronyme anglais de Laser Induced Breakdown Spectroscopy) est une technique d'analyse élémentaire basée sur l'émission d'un plasma issu de l'interaction laser-matière. Elle permet en principe une détection de l'ensemble des éléments du tableau périodique avec une sensibilité typiquement de l'ordre du ppm et ce sur tout type de matériaux : solides, liquides ou gazeux. Sa capacité à exploiter aussi bien le signal élémentaire que moléculaire en fait un candidat crédible à l'identification des matériaux organiques, par exemple dans le domaine du tri des déchets plastiques où les techniques d'analyses usuelles peinent à remplir toutes les contraintes liées à cette question. Cependant, le plasma induit par laser est un phénomène transitoire et correspondant à un milieu inhomogène parfois difficile à maitriser, notamment en comparaison avec un plasma à couplage inductif. En conséquence, la LIBS reste aujourd'hui marginale dans les applications où une information fiable et souvent quantitative est requise. Ce travail doctoral, fruit d'un partenariat entre le CRITT Matériaux Alsace et l'Institut Lumière Matière de Lyon dans le cadre 'un financement CIFRE, se propose d'étudier ces deux problématiques. Un nouvel instrument LIBS est tout d'abord présenté. Articulé autour de nombreux outils de contrôle pilotés par un logiciel dédié, il a permis de limiter considérablement les fluctuations du signal LIBS liées aux divers paramètres impliqués dans le processus d'ablation laser (énergie du laser, position de l'échantillon, position de la détection…). L'efficacité de cet instrument est montrée à travers une étude de quantification d'éléments en trace dans des matrices de verre / Laser Induced Breakdown Spectroscopy (LIBS) is an analytical technique based on the emission of a plasma arising from the laser-matter interaction. All the elements of the periodic table can be detected with a detection limit close to the ppm, regardless of the nature of sample: solid, liquid or gas. LIBS can perform elemental as well as molecular analysis, which makes it a trustworthy technique for the identification of organic materials, especially with reference to plastic waste sorting where the established techniques experience some difficulties to fulfill all the requirements of this issue. Nevertheless, the laser-induced plasma is a transient and inhomogeneous process regularly hard to master in comparison with an inductively coupled plasma. As a consequence, LIBS technique still remains marginal for the applications demanding a reliable and frequently quantitative information. This doctoral research, which falls within the framework of a partnership between the CRITT Matériaux Alsace and the Institut Lumière Matière in Lyon, proposes to examine the two issues mentioned above. A new LIBS instrument is first given. It is organized around several monitoring tools driven by a dedicated software which allowed us to considerably reduce the fluctuations of the LIBS signal coming from the different factors involved in the process of laser ablation (laser energy, sample and detection positions, etc…). The efficiency of this new LIBS instrument is then illustrated through the example of the quantification of trace elements in glass matrices

Ce travail a pour point de départ une analyse quantitative de l’objet »storyboard » faite sur un large corpus. La taxinomie ainsi établie, croisée avec une approche qualitative, nous permet de mettre en lumière des codes de communication et des invariants inhérents à ce dispositif de pré-production. Cela aboutit, dans un premier temps, à la définition de l’outil storyboard parmi les autres dessins de préparation cinématographique. Le classement des données en fonction de leur période apporte ensuite une perspective nouvelle à l’analyse : c’est par l’objet, trace de sa propre histoire et vecteur de pensées, que se dessinent les interactions sociales et la professionnalisation de ceux qui le confectionnent. L’étude porte sur deux conceptions différentes de la pratique du storyboard, d’un côté et de l’autre de l’Atlantique. / The starting point of this work involves a quantitative analysis of the object »storyboard » applied on a large corpus. The taxonomy here established, crossed witha qualitative approach, allows one to reveal communication codes and invariantsinherent to this preproduction device. This first leads to the definition of the toolstoryboard among other film preparation drawings. The classification of the dataaccording to its period then brings a new perspective to the analysis: it is through theobject, vestige of its own story and vector of ideas, from which are drawn the socialinteractions and the professionalization of those who manufacture it. The studyconcerns two different conceptions of the storyboarding practice, on both sides of theAtlantic Ocean

The master thesis The media construction of feminity according to women's lifestyle magazines in years 2006 and 2016 is concerned with values, that women's lifestyle magazines present to women. These values concern with topics such relationships, sexuality, face, health and succees in career. The thesis will examine, in which roles women appear in these magazines. The aim of the thesis is to present a picture of an ideal woman, as the women's lifestyle magazines construct it. The theoretical part is devided into five chapters. The first chapter is focused on historical development of magazine publishing in Czech Republic since 1989. The second chapter describes development of trends in the media content in the last decade. The third part deals with the media and lifestyle magazines ownership. The fourth part introduces a general content of women's lifestyle magazines. The last chapter is focused on the role of women and women's lifestyle magazines in the current society. It discusses e. g. the term "double burden". The analytical part combines qualitative and quantitative content research and its main objective is to describe, how the term feminity and the women values (relationships, sexuality, face, health and career) change in women's lifestyle magazines between years 2006 and 2016. The chosen...

Research paper entitled 'Artistic journals in the Czech Republic in the period 1990-1999' analyzes artistic journals 'Ateliér', 'Detail', 'Umělec', 'Umění' and 'Výtvarné umění', which were published between 1990 and 1999. The overarching aim of the paper is to provide readers with an overview of the formal and content-thematic characteristics of the given period as represented in the addressed journals. Quantitative content analysis of selected issues of the journals was applied as a research tool. The journals were also researched from the chronological perspective that examined the development of formal signs of each of the journals. The paper compares the basic characteristics and specifics of the journals, as well as the similarities and differences between them. Historical context of the journals with an emphasis on their beginnings, culture and art is provided for the readers. It provides the readers with an insight into the authorship of articles, thematic and content structure, structure of their journalistic departments, structure of publicized contributions and the formal outlook of the journals.

In this study the researcher set out to determine the levels and the causes of workplace stress, as well as the consequences of stress in terms of witnessed and experienced aggression in the workplace, anxiety, depression, and worry for a sample of 205 subjects. To achieve this, the following tests were used: 1) Experience of Work and Life Circumstances Questionnaire, 2) the Aggression in the Workplace Questionnaire, 3) the IPAT Anxiety Scale,4) the Beck Depression Inventory, and 5) the Penn State Worry Questionnaire. The subjects’ ability to cope withexperienced stressors in relation to social problem solving was examined with the Social Problem-Solving Inventory-Revised. The raw data were analysed by means of the usual descriptivestatistics. In addition, inferential statistics including z-tests, t-tests, analysis of variance and posthoc analyses (Scheffé) were conducted for the following groups: total group, gender, marital status, age, organizational type, qualification and position level. Results indicate that most of the subjects in the sample experienced normal levels of stress, indicating that the participants generally experienced their circumstances within or outside the workplace as satisfactory. Generally, the results also indicated that their expectations regarding their work situation were met. With reference to the consequences of stress, the total sample reported low levels of witnessing and experiencing workplace aggression, normal levels of anxiety, low levels of depression and worry. Good overall social problem solving suggests the ability to cope with demands and stressors within and outside the workplace. Generally, Pearson correlations indicated significant relationships between a) levels of stress as experienced by subjects and b) witnessed and experienced workplace aggression, c) anxiety, d) depression, e) worry and f) social problem solving. That most of the subjects in this sample were able to deal effectively with the demands and stressors placed on them, from within and outside the workplace suggests the ability to use effective problem-focused coping involving social problem solving which for most participants, was due to a positive problem orientation and effective rational problem solving skills. These findings may be useful as part of a stress management programme to help employees deal with stress proactively by becoming more effective problem-solvers. In terms of a salutogenic paradigm, and consistent with recent developments in positive psychology, the findings indicate that more attention should be paid to possible reasons why some employees appear to cope with stress more effectively than others. / Thesis (PhD (Psychology))--University of Pretoria, 2008. / Psychology / PhD / unrestricted

[pt] Até meados dos anos 90 medidas para mensurar os riscos de mercado e crédito foram as maiores fontes de pesquisas acadêmicas em finanças, impulsionadas principalmente pela indústria financeira. Foi então que alguns eventos envolvendo fraudes e erros humanos causaram perdas catastróficas, inclusive a bancarrota de grandes instituições pelo mundo. Em decorrência destes eventos, surge um imenso e ainda pouco explorado campo de pesquisa para outro tipo de risco, o risco operacional. Entende-se que o Risco Operacional está associado à possíveis perdas como resultado de sistemas e/ou controles inadequados, falhas de gerenciamento e erros humanos. Em função de suas características, o risco operacional está presente em qualquer indústria, porém a maior parte de pesquisas acadêmicas e metodologias no âmbito prático estão voltadas para instituições financeiras. Após uma vasta pesquisa, não encontramos nenhuma referência bibliográfica semelhante à desenvolvida pelo setor bancário que pudesse elucidar o nível de maturidade de gestão de riscos operacionais em outros setores. Não encontramos sequer uma definição e classificação difundida dos riscos operacionais para demais indústrias. Desta forma, este trabalho explora os frameworks e metodologias desenvolvidas pela indústria financeira para avaliação e mensuração de riscos operacionais com objetivo de estender estes métodos para outras indústrias. Adicionalmente, aplicam-se os conceitos e metodologias levantados através de um estudo de caso em uma empresa no ramo de óleo e gás. / [en] Before 1990, market and credit risk measures were the major sources of academic research in finance field, motivated by finance industry. However as a consequence of recent financial scandals in the banking industry, involving fraud and human errors, which caused catastrophic losses and even bankruptcy of many institutions around the world, a new and unexplored kind of risk has started to be studied which is called the operational risk. It is defined as the risk of loss resulting from inadequate or failed internal processes, people and systems or from external events. Due to its risk characteristics, it is present in all kind of industry but most academic researches and methodologies are developed for financial institutions. After an extensive search, we didn’t find bibliographic references similar to those in banking sector which could elucidate the operational risk management level of maturity for other sectors. We could not even find a commom definition and classification of operational risks for other industries. Thus, this work intends to explore frameworks and methodologies, developed by financial industry for evaluation and measurement of operational risk, to see how this method can be extended for other industries. Additionally, we applied these concepts and methodologies for a sector of an oil and gas company.

Realizamos neste trabalho a automatização da nova metodologia MHCalc, desenvolvida anteriormente em nosso laboratório, que teve como resultado o programa homônimo escrito em linguagem C para ambiente GNU/Linux, o qual avalia quantitativamente a seletividade de uma determinada proteína MHC. Esta avaliação quantitativa possibilita o estabelecimento de uma escala de preferência dos resíduos de ocorrência natural para cada uma das posições de interação da fenda de apresentação do MHC; por permutação destes resíduos, podem-se derivar regras de composição para peptídeos reconhecíveis em cada alelo estudado, inclusive na ausência de dados experimentais. A metodologia desenvolvida em nosso laboratório se baseia na avaliação da seletividade de cada bolsão independentemente, através da energia de interação do mesmo com cada aminoácido de ocorrência natural. O programa MHCalc utiliza o pacote de programas THORMM [Moret, 1998] de modelagem molecular para otimização geométrica das estruturas descritas, e tem como entrada unicamente um arquivo de coordenadas em formato POB contendo as coordenadas de um complexo MHC/peptídeo, tendo como saída uma tabela de dados contendo os resíduos de ocorrência natural em ordem de preferência para cada bolsão. Testamos o programa MHCalc para o complexo formado pela molécula HLA-DR1 (DRA DRB1 *01 01) e o peptídeo de Hemaglutinina, obtido no Brookhaven Protein Data Bank com a entrada 10LH, sendo este sistema escolhido por ser altamente estudado e com abundância de dados experimentais e teóricos para comparação de resultados. Até o presente momento obtivemos os dados referentes ao bolsão 1, os quais estão em pleno acordo com a literatura. / In this work we automated the new methodology MHCalc, developed previously in our laboratory, which resulted in the computational program with the same name, written in C language to GNU/Linux environment. The program MHCalc evaluates quantitatively the selectivity of a given MHC protein. This quantitative evaluation allows the establishment of a preference scale of the naturally occurring residues for each pocket of a MHC molecule. From such a study it may be derived composition rules to peptides recognizable in each allele studied, even in the absence of experimental data. The methodology developed in our laboratory is based on the selectivity evaluation of each pocket independently, through its interaction energy with each naturally occurring amino acid. The program MHCalc uses the molecular modeling package THORMM [Moret, 1998] to optimize the geometry of the structures described below, and needs as only entry the PDS like file with the coordinates of the MHC/peptide complex. The program finishes printing out a data file containing the naturally occurring residues in preference order for each pocket and the data used to order these residues. We tested the program MHCalc to the complex molecule HLA-DR1 (DRA DRB1 *01 01) with the Hemaglutinin peptide, obtained at the BrookHaven Prote in Data Bank (1 DLH entry). This system was chosen because it has been highly studied and so offers abundant experimental and theoretical data to compare our results. We obtained data referring to pocket 1 so far, which is in full agreement with the literature.